RLG00000030385

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0009834
Physical Location & Seq
Forward (+)
60499374 .. 60512163
12790 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030385

Sequence Viewer

Length: 1137 bp
ATGGGTTTTAGGATACAAACGAGGTGCATTTGGAATAAGGCTCAGAAGAGAGCCACTACAAAGAAAGTTGGGATCATGAAACAAATTGAAAGTGATGGAAAGCATCTCTGTTCTGTTGTGTATGGTTCACTTCCACCAGAGACCCGAACAAGACAGGCAACGATGTTCAATGATGCAAGCAGTGAGTTGGATGTTCTAGTAGCAAGTGATGCCATCGGGATGGGTCTCAATCTGAACATTTCACGAATCATATTTTCAACAATGACAAAATTTGATGGTATTGAGAACCGGGAACTCTCTGCACCGGAGATCAAACAAATTGCAGGAAGAGCTGGCAGGTATGGATCAAGGTTTCCTGTTGGCGAAGTGACTTGTTTAGATGCAGCTGATCTGCCTCTTCTTCATTCATCACTAAAATCCCCATCTCCGACTCTTGAGCGTACACATCTGCGGAGTGAGTATCCTGAGAAGAATAAAAGGAAACTTTGTGAATCTTCATTGCTGAAAAGGAAAAGTATTACGGGACATGGAAACCAGATCATAGGGGAAGATGCTGGTGCAATTCATCTCCCAAATCCAATGAATGGGTTCGGGACTCCAGCTGAACTCTGTCAAATCCACAGAAGTCTTCCAAAGGCAGCCACAGGACCTCCTTATGCTGGTGCAACAATTACTGAGAGGATCATGAAGGCTCTCAAAGACTATGACGAAGATGAGAAAGTGAAAGAAAAAGGTGGCTCCACTGGAGCCGGATCAAGTGGAAATGCTTTTGGGGTTCCCATGGGACCTGACAAGGAGATAGCTCTTTACCGGCTTGGTATCCCGATGAAGAATATTGTGTCAGTGGAGATTTCAGAGGTGAGCAGAAATGTTGTGAGGTGTTGGTGGGAGCAGACAAACCAGAGAGGGAACTTGTATCACCTTGCTGATGTGCAAGAGTTGAATGCTGACCGCTTAGAGCATTATATCAGTTCCTTTGGTGGGTTTGATCTAGAGATTTGTGGCAGTCCATGTAACAATCTTGCGGGTAGCAACAGGCACCATCGGGATGGGCTTGAGGGTAAAGAATCTTCTCTTTTTTATGACTATTTTCGTATATTGGATTTGGTCAAGGGTATAATGGCAAGATACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

379

Amino Acids

41.73

Weight (kDa)

8.66

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 22 - 115 4.7e-11 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 327
AccB1I GGYRCC 1 cut(s) 1038
AccB7I CCANNNNNTGG 1 cut(s) 584
AciI CCGC 3 cut(s) 451, 952, 1025
AclWI GGATC 4 cut(s) 80, 352, 689, 760
AcsI RAATTY 1 cut(s) 269
AfaI GTAC 1 cut(s) 442
AfiI CCNNNNNNNGG 3 cut(s) 584, 659, 981
AgsI TTSAA 4 cut(s) 89, 169, 258, 943
AluBI AGCT 4 cut(s) 332, 386, 602, 803
AluI AGCT 4 cut(s) 332, 386, 602, 803
Alw26I GTCTC 2 cut(s) 134, 230
AlwI GGATC 4 cut(s) 80, 352, 689, 760
ApeKI GCWGC 2 cut(s) 383, 638
ApoI RAATTY 1 cut(s) 269
Asp700I GAANNNNTTC 1 cut(s) 587
AspS9I GGNCC 2 cut(s) 647, 785
AsuC2I CCSGG 1 cut(s) 290
AsuHPI GGTGA 2 cut(s) 871, 911
AvaII GGWCC 2 cut(s) 647, 785
BanI GGYRCC 1 cut(s) 1038
BarI GAAGNNNNNNTAC 2 cut(s) 1054, 1086
BbsI GAAGAC 1 cut(s) 620
BbvI GCAGC 2 cut(s) 395, 650
BccI CCATC 7 cut(s) 89, 214, 221, 269, 430, 1043, 1050
BciVI GTATCC 3 cut(s) 6, 471, 830
BcnI CCSGG 1 cut(s) 290
BcoDI GTCTC 2 cut(s) 134, 230
BfaI CTAG 2 cut(s) 197, 992
BfuAI ACCTGC 1 cut(s) 327
BfuI GTATCC 3 cut(s) 6, 471, 830
BisI GCNGC 2 cut(s) 384, 639
BlsI GCNGC 2 cut(s) 385, 640
Bme1390I CCNGG 1 cut(s) 290
Bme18I GGWCC 2 cut(s) 647, 785
BmgT120I GGNCC 2 cut(s) 647, 785
BmiI GGNNCC 5 cut(s) 739, 748, 777, 786, 1040
BmrFI CCNGG 1 cut(s) 290
BmsI GCATC 5 cut(s) 112, 163, 199, 370, 541
BpiI GAAGAC 1 cut(s) 620
BpmI CTGGAG 2 cut(s) 582, 765
BpuEI CTTGAG 2 cut(s) 455, 1076
BpuMI CCSGG 1 cut(s) 290
BsaI GGTCTC 2 cut(s) 134, 230
BsaJI CCNNGG 1 cut(s) 780
BsaWI WCCGGW 1 cut(s) 304
BsaXI ACNNNNNCTCC 2 cut(s) 634, 664
Bsc4I CCNNNNNNNGG 3 cut(s) 584, 659, 981
Bse118I RCCGGY 1 cut(s) 810
Bse1I ACTGG 1 cut(s) 748
Bse3DI GCAATG 1 cut(s) 497
BseDI CCNNGG 1 cut(s) 780
BseGI GGATG 3 cut(s) 196, 225, 1054
BseLI CCNNNNNNNGG 3 cut(s) 584, 659, 981
BseMI GCAATG 1 cut(s) 497
BseMII CTCAG 3 cut(s) 56, 456, 666
BseNI ACTGG 1 cut(s) 748
BseXI GCAGC 2 cut(s) 395, 650
BsgI GTGCAG 1 cut(s) 285
BshNI GGYRCC 1 cut(s) 1038
BsiSI CCGG 4 cut(s) 289, 305, 750, 811
BslFI GGGAC 3 cut(s) 537, 607, 798
BslI CCNNNNNNNGG 3 cut(s) 584, 659, 981
BsmAI GTCTC 2 cut(s) 134, 230
BsmFI GGGAC 3 cut(s) 537, 607, 798
BsmI GAATGC 1 cut(s) 949
Bso31I GGTCTC 2 cut(s) 134, 230
Bsp143I GATC 8 cut(s) 72, 309, 344, 388, 537, 681, 752, 988
Bsp19I CCATGG 1 cut(s) 780
BspACI CCGC 3 cut(s) 451, 952, 1025
BspCNI CTCAG 3 cut(s) 55, 457, 667
BspHI TCATGA 2 cut(s) 75, 684
BspLI GGNNCC 5 cut(s) 739, 748, 777, 786, 1040
BspMI ACCTGC 1 cut(s) 327
BspPI GGATC 4 cut(s) 80, 352, 689, 760
BspQI GCTCTTC 1 cut(s) 322
BspT107I GGYRCC 1 cut(s) 1038
BspTNI GGTCTC 2 cut(s) 134, 230
BsrDI GCAATG 1 cut(s) 497
BsrFI RCCGGY 1 cut(s) 810
BsrI ACTGG 1 cut(s) 748
BssAI RCCGGY 1 cut(s) 810
BssECI CCNNGG 1 cut(s) 780
BssMI GATC 8 cut(s) 72, 309, 344, 388, 537, 681, 752, 988
BssT1I CCWWGG 1 cut(s) 780
Bst6I CTCTTC 3 cut(s) 41, 322, 402
BstAPI GCANNNNNTGC 1 cut(s) 209
BstC8I GCNNGC 2 cut(s) 178, 334
BstDEI CTNAG 4 cut(s) 42, 465, 675, 955
BstDSI CCRYGG 1 cut(s) 780
BstF5I GGATG 3 cut(s) 196, 225, 1054
BstKTI GATC 8 cut(s) 75, 312, 347, 391, 540, 684, 755, 991
BstMAI GTCTC 2 cut(s) 134, 230
BstMBI GATC 8 cut(s) 72, 309, 344, 388, 537, 681, 752, 988
BstMWI GCNNNNNNNGC 2 cut(s) 209, 329
BstSCI CCNGG 1 cut(s) 288
BstV1I GCAGC 2 cut(s) 395, 650
BstV2I GAAGAC 1 cut(s) 620
BstXI CCANNNNNNTGG 2 cut(s) 220, 1049
BsuI GTATCC 3 cut(s) 6, 471, 830
BtgI CCRYGG 1 cut(s) 780
BtsCI GGATG 3 cut(s) 196, 225, 1054
BtsI GCAGTG 1 cut(s) 187
BtsIMutI CAGTG 3 cut(s) 187, 741, 849
BveI ACCTGC 1 cut(s) 327
Cac8I GCNNGC 2 cut(s) 178, 334
CciI TCATGA 2 cut(s) 75, 684
Cfr10I RCCGGY 1 cut(s) 810
Cfr13I GGNCC 2 cut(s) 647, 785
Csp6I GTAC 1 cut(s) 441
CviAII CATG 5 cut(s) 76, 527, 685, 781, 1011
CviQI GTAC 1 cut(s) 441
DdeI CTNAG 4 cut(s) 42, 465, 675, 955
DpnI GATC 8 cut(s) 74, 311, 346, 390, 539, 683, 754, 990
DpnII GATC 8 cut(s) 72, 309, 344, 388, 537, 681, 752, 988
Eam1104I CTCTTC 3 cut(s) 41, 322, 402
EarI CTCTTC 3 cut(s) 41, 322, 402
Eco130I CCWWGG 1 cut(s) 780
Eco31I GGTCTC 2 cut(s) 134, 230
Eco47I GGWCC 2 cut(s) 647, 785
EcoO109I RGGNCCY 2 cut(s) 647, 785
EcoT14I CCWWGG 1 cut(s) 780
ErhI CCWWGG 1 cut(s) 780
FaeI CATG 5 cut(s) 79, 530, 688, 784, 1014
FaqI GGGAC 3 cut(s) 537, 607, 798
FatI CATG 5 cut(s) 75, 526, 684, 780, 1010
FauI CCCGC 1 cut(s) 1018
Fnu4HI GCNGC 2 cut(s) 384, 639
FokI GGATG 3 cut(s) 203, 232, 1061
Fsp4HI GCNGC 2 cut(s) 384, 639
FspBI CTAG 2 cut(s) 197, 992
GluI GCNGC 2 cut(s) 384, 639
GsuI CTGGAG 2 cut(s) 582, 765
HapII CCGG 4 cut(s) 289, 305, 750, 811
Hin1II CATG 5 cut(s) 79, 530, 688, 784, 1014
HinfI GANTC 5 cut(s) 246, 430, 491, 595, 1067
HpaII CCGG 4 cut(s) 289, 305, 750, 811
HphI GGTGA 2 cut(s) 871, 911
Hpy166II GTNNAC 2 cut(s) 128, 443
Hpy188I TCNGA 4 cut(s) 45, 234, 429, 856
Hpy8I GTNNAC 2 cut(s) 128, 443
HpyAV CCTTC 1 cut(s) 682
HpyCH4V TGCA 8 cut(s) 27, 176, 302, 323, 383, 560, 665, 934
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 329
HpyF3I CTNAG 4 cut(s) 42, 465, 675, 955
Hsp92II CATG 5 cut(s) 79, 530, 688, 784, 1014
Kzo9I GATC 8 cut(s) 72, 309, 344, 388, 537, 681, 752, 988
LguI GCTCTTC 1 cut(s) 322
LmnI GCTCC 3 cut(s) 743, 746, 889
Lsp1109I GCAGC 2 cut(s) 395, 650
LweI GCATC 5 cut(s) 112, 163, 199, 370, 541
MaeI CTAG 2 cut(s) 197, 992
MaeIII GTNAC 2 cut(s) 367, 1013
MalI GATC 8 cut(s) 74, 311, 346, 390, 539, 683, 754, 990
MboI GATC 8 cut(s) 72, 309, 344, 388, 537, 681, 752, 988
MfeI CAATTG 1 cut(s) 1132
MluCI AATT 6 cut(s) 84, 269, 318, 561, 669, 1132
MlyI GAGTC 2 cut(s) 424, 589
MmeI TCCRAC 2 cut(s) 168, 452
MnlI CCTC 8 cut(s) 15, 405, 660, 672, 850, 870, 899, 1051
MroXI GAANNNNTTC 1 cut(s) 587
MslI CAYNNNNRTG 2 cut(s) 218, 1047
MspA1I CMGCKG 2 cut(s) 386, 602
MspI CCGG 4 cut(s) 289, 305, 750, 811
MspR9I CCNGG 1 cut(s) 290
MunI CAATTG 1 cut(s) 1132
Mva1269I GAATGC 1 cut(s) 949
MwoI GCNNNNNNNGC 2 cut(s) 209, 329
NciI CCSGG 1 cut(s) 290
NcoI CCATGG 1 cut(s) 780
NdeII GATC 8 cut(s) 72, 309, 344, 388, 537, 681, 752, 988
NlaIII CATG 5 cut(s) 79, 530, 688, 784, 1014
NlaIV GGNNCC 5 cut(s) 739, 748, 777, 786, 1040
NmuCI GTSAC 1 cut(s) 367
PagI TCATGA 2 cut(s) 75, 684
PciSI GCTCTTC 1 cut(s) 322
PctI GAATGC 1 cut(s) 949
PdmI GAANNNNTTC 1 cut(s) 587
PfeI GAWTC 3 cut(s) 246, 491, 1067
PflMI CCANNNNNTGG 1 cut(s) 584
PkrI GCNGC 2 cut(s) 385, 640
PleI GAGTC 2 cut(s) 424, 589
PpsI GAGTC 2 cut(s) 424, 589
PpuMI RGGWCCY 2 cut(s) 647, 785
Psp5II RGGWCCY 2 cut(s) 647, 785
PspN4I GGNNCC 5 cut(s) 739, 748, 777, 786, 1040
PspPI GGNCC 2 cut(s) 647, 785
PspPPI RGGWCCY 2 cut(s) 647, 785
PvuII CAGCTG 2 cut(s) 386, 602
RsaI GTAC 1 cut(s) 442
RsaNI GTAC 1 cut(s) 441
RseI CAYNNNNRTG 2 cut(s) 218, 1047
SapI GCTCTTC 1 cut(s) 322
SatI GCNGC 2 cut(s) 384, 639
Sau3AI GATC 8 cut(s) 72, 309, 344, 388, 537, 681, 752, 988
Sau96I GGNCC 2 cut(s) 647, 785
SchI GAGTC 2 cut(s) 424, 589
ScrFI CCNGG 1 cut(s) 290
SfaNI GCATC 5 cut(s) 112, 163, 199, 370, 541
SinI GGWCC 2 cut(s) 647, 785
SmiMI CAYNNNNRTG 2 cut(s) 218, 1047
SmlI CTYRAG 2 cut(s) 434, 1055
SmoI CTYRAG 2 cut(s) 434, 1055
Sse9I AATT 6 cut(s) 84, 269, 318, 561, 669, 1132
SsiI CCGC 3 cut(s) 451, 952, 1025
SspI AATATT 1 cut(s) 835
SspMI CTAG 2 cut(s) 197, 992
StyD4I CCNGG 1 cut(s) 288
StyI CCWWGG 1 cut(s) 780
TasI AATT 6 cut(s) 84, 269, 318, 561, 669, 1132
TfiI GAWTC 3 cut(s) 246, 491, 1067
TscAI CASTG 3 cut(s) 187, 748, 849
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 2 cut(s) 383, 638
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 8 cut(s) 92, 392, 396, 486, 554, 596, 701, 842
TspRI CASTG 3 cut(s) 187, 748, 849
Van91I CCANNNNNTGG 1 cut(s) 584
VpaK11BI GGWCC 2 cut(s) 647, 785
XapI RAATTY 1 cut(s) 269
XbaI TCTAGA 1 cut(s) 991
XmnI GAANNNNTTC 1 cut(s) 587
XspI CTAG 2 cut(s) 197, 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.