RLG00000033252

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013018
Physical Location & Seq
Reverse (-)
24984844 .. 24993264
8421 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033252

Sequence Viewer

Length: 1170 bp
ATGAAGATCAAAGTTAGAAGCCTATCAAGAATTTGGTTACGGCTCACCGGCTGTCTGCGCCACAAACAAGATGGTGATGACGATGATGATGATTTGTGGTCCAAGCAACTGCCTGATGATATCATGAAATTGACTACCTCCACTAGTGTCGTGACGTATGCCCTTCTTGGCGAGCAGCAATTGACAGCAAGCGTTGCCTTCCTGCTCCACATCAACTTGATCACTGAAAAATCATGGACCCTGATTGAGGCCGTAAATGAAGAGCAGAAGGTTCTCGAATTTTGCAATGTAGAAATCCTTGATGATAAGCTATATGCTGTAACCAGCAATGCATGGGGCTTTGTAATGGTGTTTCACATCAAATCAGATGCCAATGCTGGACTTTCTACCTACACGGCTGAAAGGGTGGTTTTACATCTCCCTATTAATACCGGAGAACATTATCTTAGCAGATTTCCCCTCTTTTACAGGAATGTTTGGTACCCAGCAAAAGATTTTGAATCCAAGGAGTTGTTCATTATTTTGGGCAACACCTCTCGCCCTCAAAACCGTACCGAAGGATTTCAATTGTGGAAGTTGGAGTGTAATATTACTGGTGGCCCTCGGTGGGTTGAGATTTTCGATCTTGGTAATAGAACATTGTTCGTGAGTGGGGATAACTATAAGTTCATCTCTTCCACCAGTGGGTCCCTAGGCTCACAGTCATTAAGATATAGCATAAGGAGACTGGAAAGACTAGAAGATCTTGTATTCTACGACGACATCAACCGAAGCAATGGCATCCGGACAAGGTTAATATGCTATTTGAATATTGTTACCATACCTTTTGCTGCCACACCTACTCAAGAGGAACCCCAAAACATGCAAGAGATGGACGTTGGTGATCATGGTCCTAATGAACCCCAAGCCATGCACGTTGGTGATGTTGTTCGTAATGAATCCTTGCACATTGATCCGCAAGTGGCAGCTGCACCACTTTTAGGGTTTGATTTAATTATGCCCGAGCCAGATGCTAGCGACGTCAACCACAACGATATCAACGGTGAAAAAGGAGCTAAAACGGCGTCGTCCGACAGAGGGATGAGTGTGGGGAAGAAGGCCGGGATCGGACTCGGAGTGATCATCGGGGTTGGGTTGGTCGGACTCGAAGCTTTTTGGGAAGTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

390

Amino Acids

43.65

Weight (kDa)

5.04

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 60 - 228 1e-07 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013018)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28440 AT3G45230 AT5G60630
fragaria_vesca FvH4_3g32420
prunus_persica Prupe.2G214100_v2.0.a1 Prupe.6G101100_v2.0.a1
pyrus_communis pycom11g11220
rosa_chinensis RchiOBHm_Chr5g0059401
rosa_laevigata RLG00000033252
rosa_multiflora Rmu_sc0004458.1_g000004
rosa_roxburghii Rroxscaffold_1G00021080
rosa_rugosa Rorug05G0322700
rosa_samantha Rh5AG387200 Rh5BG398500 Rh5CG422600 Rh5DG414100 Rh6CG137900
rosa_wichuraiana Rw5G036430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1023
Acc65I GGTACC 1 cut(s) 480
AccB1I GGYRCC 1 cut(s) 480
AccIII TCCGGA 1 cut(s) 783
AciI CCGC 1 cut(s) 956
AclWI GGATC 2 cut(s) 947, 1112
AcsI RAATTY 2 cut(s) 30, 278
AcyI GRCGYC 2 cut(s) 1020, 1064
AfaI GTAC 2 cut(s) 482, 553
AfiI CCNNNNNNNGG 5 cut(s) 247, 607, 684, 980, 1077
AgsI TTSAA 3 cut(s) 500, 566, 808
AhlI ACTAGT 1 cut(s) 143
AjuI GAANNNNNNNTTGG 2 cut(s) 497, 529
AleI CACNNNNGTG 1 cut(s) 918
AluBI AGCT 4 cut(s) 310, 968, 1055, 1151
AluI AGCT 4 cut(s) 310, 968, 1055, 1151
Alw26I GTCTC 1 cut(s) 718
AlwI GGATC 2 cut(s) 947, 1112
Ama87I CYCGRG 1 cut(s) 1001
Aor13HI TCCGGA 1 cut(s) 783
AoxI GGCC 3 cut(s) 249, 598, 1098
ApeKI GCWGC 4 cut(s) 175, 830, 965, 968
ApoI RAATTY 2 cut(s) 30, 278
AseI ATTAAT 1 cut(s) 426
Asp700I GAANNNNTTC 1 cut(s) 561
Asp718I GGTACC 1 cut(s) 480
AspA2I CCTAGG 1 cut(s) 691
AspLEI GCGC 1 cut(s) 60
AspS9I GGNCC 5 cut(s) 99, 237, 599, 687, 890
AsuC2I CCSGG 1 cut(s) 1102
AsuHPI GGTGA 5 cut(s) 37, 86, 893, 932, 1055
AsuNHI GCTAGC 1 cut(s) 1013
AvaI CYCGRG 1 cut(s) 1001
AvaII GGWCC 4 cut(s) 99, 237, 687, 890
AvrII CCTAGG 1 cut(s) 691
BanI GGYRCC 1 cut(s) 480
BbvI GCAGC 4 cut(s) 187, 817, 955, 977
BccI CCATC 2 cut(s) 65, 865
BceAI ACGGC 4 cut(s) 56, 236, 411, 1077
BcgI CGANNNNNNTGC 2 cut(s) 992, 1026
BclI TGATCA 3 cut(s) 219, 883, 1119
BcnI CCSGG 1 cut(s) 1102
BcoDI GTCTC 1 cut(s) 718
BcuI ACTAGT 1 cut(s) 143
BfaI CTAG 4 cut(s) 144, 692, 737, 1014
BglII AGATCT 1 cut(s) 742
BisI GCNGC 4 cut(s) 176, 831, 966, 969
BlnI CCTAGG 1 cut(s) 691
BlsI GCNGC 4 cut(s) 177, 832, 967, 970
Bme1390I CCNGG 1 cut(s) 1102
Bme18I GGWCC 4 cut(s) 99, 237, 687, 890
BmeT110I CYCGRG 1 cut(s) 1001
BmgT120I GGNCC 5 cut(s) 99, 237, 599, 687, 890
BmiI GGNNCC 5 cut(s) 239, 482, 688, 689, 852
BmrFI CCNGG 1 cut(s) 1102
BmsI GCATC 3 cut(s) 358, 789, 1000
BmtI GCTAGC 1 cut(s) 1017
BpuEI CTTGAG 1 cut(s) 828
BpuMI CCSGG 1 cut(s) 1102
BsaHI GRCGYC 2 cut(s) 1020, 1064
BsaJI CCNNGG 3 cut(s) 504, 602, 691
BsaWI WCCGGW 2 cut(s) 431, 783
Bsc4I CCNNNNNNNGG 5 cut(s) 247, 607, 684, 980, 1077
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 3 cut(s) 598, 681, 732
Bse3DI GCAATG 3 cut(s) 292, 334, 781
BseAI TCCGGA 1 cut(s) 783
BseDI CCNNGG 3 cut(s) 504, 602, 691
BseGI GGATG 2 cut(s) 780, 1086
BseLI CCNNNNNNNGG 5 cut(s) 247, 607, 684, 980, 1077
BseMI GCAATG 3 cut(s) 292, 334, 781
BseNI ACTGG 3 cut(s) 598, 681, 732
BseXI GCAGC 4 cut(s) 187, 817, 955, 977
BseYI CCCAGC 1 cut(s) 484
BsgI GTGCAG 1 cut(s) 954
BshFI GGCC 3 cut(s) 251, 600, 1100
BshNI GGYRCC 1 cut(s) 480
BsiHKCI CYCGRG 1 cut(s) 1001
BsiSI CCGG 4 cut(s) 48, 432, 784, 1101
BslFI GGGAC 1 cut(s) 673
BslI CCNNNNNNNGG 5 cut(s) 247, 607, 684, 980, 1077
BsmAI GTCTC 1 cut(s) 718
BsmFI GGGAC 1 cut(s) 673
BsnI GGCC 3 cut(s) 251, 600, 1100
BsoBI CYCGRG 1 cut(s) 1001
Bsp13I TCCGGA 1 cut(s) 783
Bsp143I GATC 8 cut(s) 6, 219, 622, 742, 883, 952, 1104, 1119
BspACI CCGC 1 cut(s) 956
BspANI GGCC 3 cut(s) 251, 600, 1100
BspEI TCCGGA 1 cut(s) 783
BspHI TCATGA 1 cut(s) 123
BspLI GGNNCC 5 cut(s) 239, 482, 688, 689, 852
BspOI GCTAGC 1 cut(s) 1017
BspPI GGATC 2 cut(s) 947, 1112
BspQI GCTCTTC 1 cut(s) 255
BspT107I GGYRCC 1 cut(s) 480
BsrDI GCAATG 3 cut(s) 292, 334, 781
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 3 cut(s) 598, 681, 732
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 3 cut(s) 504, 602, 691
BssMI GATC 8 cut(s) 6, 219, 622, 742, 883, 952, 1104, 1119
BssNI GRCGYC 2 cut(s) 1020, 1064
BssT1I CCWWGG 2 cut(s) 504, 691
Bst4CI ACNGT 3 cut(s) 551, 702, 1043
Bst6I CTCTTC 2 cut(s) 255, 679
BstACI GRCGYC 2 cut(s) 1020, 1064
BstAPI GCANNNNNTGC 1 cut(s) 194
BstC8I GCNNGC 3 cut(s) 173, 190, 1015
BstDEI CTNAG 1 cut(s) 446
BstENI CCTNNNNNAGG 1 cut(s) 245
BstF5I GGATG 2 cut(s) 780, 1086
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 8 cut(s) 9, 222, 625, 745, 886, 955, 1107, 1122
BstMAI GTCTC 1 cut(s) 718
BstMBI GATC 8 cut(s) 6, 219, 622, 742, 883, 952, 1104, 1119
BstMWI GCNNNNNNNGC 3 cut(s) 57, 194, 1061
BstNSI RCATGY 1 cut(s) 865
BstSCI CCNGG 1 cut(s) 1100
BstV1I GCAGC 4 cut(s) 187, 817, 955, 977
BstX2I RGATCY 1 cut(s) 742
BstYI RGATCY 1 cut(s) 742
BsuRI GGCC 3 cut(s) 251, 600, 1100
BtsCI GGATG 2 cut(s) 780, 1086
BtsIMutI CAGTG 2 cut(s) 222, 688
Cac8I GCNNGC 3 cut(s) 173, 190, 1015
CciI TCATGA 1 cut(s) 123
CfoI GCGC 1 cut(s) 60
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 5 cut(s) 99, 237, 599, 687, 890
CseI GACGC 1 cut(s) 1053
Csp6I GTAC 2 cut(s) 481, 552
CviAII CATG 6 cut(s) 124, 234, 333, 862, 887, 910
CviQI GTAC 2 cut(s) 481, 552
DdeI CTNAG 1 cut(s) 446
DpnI GATC 8 cut(s) 8, 221, 624, 744, 885, 954, 1106, 1121
DpnII GATC 8 cut(s) 6, 219, 622, 742, 883, 952, 1104, 1119
Eam1104I CTCTTC 2 cut(s) 255, 679
EarI CTCTTC 2 cut(s) 255, 679
Eco130I CCWWGG 2 cut(s) 504, 691
Eco32I GATATC 2 cut(s) 121, 1036
Eco47I GGWCC 4 cut(s) 99, 237, 687, 890
Eco88I CYCGRG 1 cut(s) 1001
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 687
EcoRV GATATC 2 cut(s) 121, 1036
EcoT14I CCWWGG 2 cut(s) 504, 691
EcoT22I ATGCAT 1 cut(s) 334
ErhI CCWWGG 2 cut(s) 504, 691
FaeI CATG 6 cut(s) 127, 237, 336, 865, 890, 913
FaqI GGGAC 1 cut(s) 673
FatI CATG 6 cut(s) 123, 233, 332, 861, 886, 909
FbaI TGATCA 3 cut(s) 219, 883, 1119
Fnu4HI GCNGC 4 cut(s) 176, 831, 966, 969
FokI GGATG 2 cut(s) 767, 1093
Fsp4HI GCNGC 4 cut(s) 176, 831, 966, 969
FspBI CTAG 4 cut(s) 144, 692, 737, 1014
GlaI GCGC 1 cut(s) 59
GluI GCNGC 4 cut(s) 176, 831, 966, 969
GsaI CCCAGC 1 cut(s) 488
HaeIII GGCC 3 cut(s) 251, 600, 1100
HapII CCGG 4 cut(s) 48, 432, 784, 1101
HgaI GACGC 1 cut(s) 1053
HhaI GCGC 1 cut(s) 60
Hin1I GRCGYC 2 cut(s) 1020, 1064
Hin1II CATG 6 cut(s) 127, 237, 336, 865, 890, 913
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HincII GTYRAC 1 cut(s) 1024
HindII GTYRAC 1 cut(s) 1024
HindIII AAGCTT 1 cut(s) 1149
HinfI GANTC 4 cut(s) 500, 938, 1110, 1143
HpaII CCGG 4 cut(s) 48, 432, 784, 1101
HphI GGTGA 5 cut(s) 37, 86, 893, 932, 1055
Hpy166II GTNNAC 1 cut(s) 1024
Hpy188I TCNGA 5 cut(s) 367, 1072, 1109, 1115, 1142
Hpy188III TCNNGA 7 cut(s) 27, 124, 151, 275, 646, 784, 845
Hpy8I GTNNAC 1 cut(s) 1024
Hpy99I CGWCG 3 cut(s) 761, 1022, 1069
HpyAV CCTTC 5 cut(s) 173, 208, 262, 551, 1090
HpyCH4III ACNGT 3 cut(s) 551, 702, 1043
HpyCH4IV ACGT 4 cut(s) 155, 876, 915, 1020
HpyCH4V TGCA 6 cut(s) 285, 332, 865, 913, 946, 971
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 194, 1061
HpyF3I CTNAG 1 cut(s) 446
HpySE526I ACGT 4 cut(s) 155, 876, 915, 1020
Hsp92I GRCGYC 2 cut(s) 1020, 1064
Hsp92II CATG 6 cut(s) 127, 237, 336, 865, 890, 913
HspAI GCGC 1 cut(s) 58
KflI GGGWCCC 1 cut(s) 687
Kpn2I TCCGGA 1 cut(s) 783
KpnI GGTACC 1 cut(s) 484
Ksp22I TGATCA 3 cut(s) 219, 883, 1119
Kzo9I GATC 8 cut(s) 6, 219, 622, 742, 883, 952, 1104, 1119
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 2 cut(s) 210, 1052
Lsp1109I GCAGC 4 cut(s) 187, 817, 955, 977
LweI GCATC 3 cut(s) 358, 789, 1000
MaeI CTAG 4 cut(s) 144, 692, 737, 1014
MaeII ACGT 4 cut(s) 155, 876, 915, 1020
MaeIII GTNAC 4 cut(s) 36, 151, 319, 814
MalI GATC 8 cut(s) 8, 221, 624, 744, 885, 954, 1106, 1121
MboI GATC 8 cut(s) 6, 219, 622, 742, 883, 952, 1104, 1119
MboII GAAGA 5 cut(s) 16, 272, 666, 752, 1105
MfeI CAATTG 2 cut(s) 179, 566
MflI RGATCY 1 cut(s) 742
MluCI AATT 6 cut(s) 30, 128, 179, 278, 566, 993
MlyI GAGTC 2 cut(s) 1104, 1137
MmeI TCCRAC 3 cut(s) 558, 1095, 1120
MnlI CCTC 8 cut(s) 148, 241, 470, 544, 552, 612, 841, 1070
Mph1103I ATGCAT 1 cut(s) 334
MroI TCCGGA 1 cut(s) 783
MroXI GAANNNNTTC 1 cut(s) 561
MseI TTAA 4 cut(s) 426, 707, 794, 992
MslI CAYNNNNRTG 1 cut(s) 918
MspA1I CMGCKG 1 cut(s) 968
MspI CCGG 4 cut(s) 48, 432, 784, 1101
MspR9I CCNGG 1 cut(s) 1102
MunI CAATTG 2 cut(s) 179, 566
MwoI GCNNNNNNNGC 3 cut(s) 57, 194, 1061
NciI CCSGG 1 cut(s) 1102
NdeII GATC 8 cut(s) 6, 219, 622, 742, 883, 952, 1104, 1119
NheI GCTAGC 1 cut(s) 1013
NlaIII CATG 6 cut(s) 127, 237, 336, 865, 890, 913
NlaIV GGNNCC 5 cut(s) 239, 482, 688, 689, 852
NmuCI GTSAC 1 cut(s) 151
NsiI ATGCAT 1 cut(s) 334
NspI RCATGY 1 cut(s) 865
OliI CACNNNNGTG 1 cut(s) 918
PagI TCATGA 1 cut(s) 123
PciSI GCTCTTC 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 561
PfeI GAWTC 2 cut(s) 500, 938
PkrI GCNGC 4 cut(s) 177, 832, 967, 970
PleI GAGTC 2 cut(s) 1104, 1137
PpsI GAGTC 2 cut(s) 1104, 1137
PpuMI RGGWCCY 1 cut(s) 687
PshBI ATTAAT 1 cut(s) 426
Psp5II RGGWCCY 1 cut(s) 687
PspFI CCCAGC 1 cut(s) 484
PspN4I GGNNCC 5 cut(s) 239, 482, 688, 689, 852
PspPI GGNCC 5 cut(s) 99, 237, 599, 687, 890
PspPPI RGGWCCY 1 cut(s) 687
PsuI RGATCY 1 cut(s) 742
PvuII CAGCTG 1 cut(s) 968
RsaI GTAC 2 cut(s) 482, 553
RsaNI GTAC 2 cut(s) 481, 552
RseI CAYNNNNRTG 1 cut(s) 918
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 4 cut(s) 426, 707, 794, 992
SatI GCNGC 4 cut(s) 176, 831, 966, 969
Sau3AI GATC 8 cut(s) 6, 219, 622, 742, 883, 952, 1104, 1119
Sau96I GGNCC 5 cut(s) 99, 237, 599, 687, 890
SchI GAGTC 2 cut(s) 1104, 1137
ScrFI CCNGG 1 cut(s) 1102
SfaNI GCATC 3 cut(s) 358, 789, 1000
SinI GGWCC 4 cut(s) 99, 237, 687, 890
SmiMI CAYNNNNRTG 1 cut(s) 918
SmlI CTYRAG 1 cut(s) 843
SmoI CTYRAG 1 cut(s) 843
SpeI ACTAGT 1 cut(s) 143
Sse9I AATT 6 cut(s) 30, 128, 179, 278, 566, 993
SsiI CCGC 1 cut(s) 956
SspI AATATT 2 cut(s) 589, 811
SspMI CTAG 4 cut(s) 144, 692, 737, 1014
StyD4I CCNGG 1 cut(s) 1100
StyI CCWWGG 2 cut(s) 504, 691
TaaI ACNGT 3 cut(s) 551, 702, 1043
TaiI ACGT 4 cut(s) 158, 879, 918, 1023
TaqI TCGA 3 cut(s) 276, 621, 1146
TasI AATT 6 cut(s) 30, 128, 179, 278, 566, 993
TfiI GAWTC 2 cut(s) 500, 938
Tru1I TTAA 4 cut(s) 426, 707, 794, 992
Tru9I TTAA 4 cut(s) 426, 707, 794, 992
TscAI CASTG 2 cut(s) 229, 688
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 4 cut(s) 175, 830, 965, 968
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 7 cut(s) 17, 140, 273, 505, 658, 912, 951
TspRI CASTG 2 cut(s) 229, 688
VpaK11BI GGWCC 4 cut(s) 99, 237, 687, 890
VspI ATTAAT 1 cut(s) 426
XagI CCTNNNNNAGG 1 cut(s) 245
XapI RAATTY 2 cut(s) 30, 278
XceI RCATGY 1 cut(s) 865
XcmI CCANNNNNNNNNTGG 1 cut(s) 68
XmaJI CCTAGG 1 cut(s) 691
XmnI GAANNNNTTC 1 cut(s) 561
XspI CTAG 4 cut(s) 144, 692, 737, 1014
ZraI GACGTC 1 cut(s) 1021
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.