RLG00000033373

Male gamete fusion factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015760
Physical Location & Seq
Forward (+)
26424400 .. 26426947
2548 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033373

Sequence Viewer

Length: 630 bp
ATGACAGAGGCTGCTTATTGTATGAATGTGATGAATAGCGGAATCAGAGAACTAGTGAAAGCCATTCACTCTACTCCCACGCAGTCCGTTCTCTATCTCTCCGGCGGAGCCTCTCAGGCGGTGGCGTGGTTACTCTCCGTTCCCGGAGCCGCAAGTACAGACTTGGAAACTGTAGTGCCTTATTCCCAAATGCTGACCAAAACCGTATTGAAAGGAATTAGTTGCCAGTTTTTGGTGTGGAAGGAAGGGACTCATTCATTTTCCATAGGAGTCACAGAAGTCATTAATACAAATCTCTTGATAGAACTAAGTGCTGATGACGTAGATTATGTCTACCAAAGGAGTCCTGGGAAAATCTTAAGCATCAATATCCCAACATTTGGGGCCCTTACTCAATTTGGAACTGCTACAATCACAGCAAAGAATACTGGTAAAGTGGAAGCATCCTATTGCTTAACGTTTGATTGCTCAAGTGATGTCACCCTTATGGAGGACTCTAATTATAATGAAGTGGATGGAGCCGAGTGCCAATTTTCTACGATGTCTACTGTCATTGATAATGGATCACAGGTACTTGTTGCACAGTGGAATAATCAAAATTCTGGCTCATTTCTAACTGACATACTCTAG

Protein Analysis

210

Amino Acids

22.5

Weight (kDa)

4.35

Isoelectric Point (pI)

28.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAP2-GCS1 PF10699 74 - 164 2.5e-24 Male gamete fusion factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 504
AccB7I CCANNNNNTGG 2 cut(s) 232, 380
AccI GTMKAC 2 cut(s) 333, 545
AciI CCGC 4 cut(s) 39, 105, 119, 150
AclI AACGTT 1 cut(s) 458
AclWI GGATC 1 cut(s) 571
AcsI RAATTY 1 cut(s) 598
AfaI GTAC 2 cut(s) 157, 573
AfiI CCNNNNNNNGG 3 cut(s) 232, 380, 490
AflII CTTAAG 1 cut(s) 358
AgsI TTSAA 1 cut(s) 211
AhlI ACTAGT 1 cut(s) 52
AjnI CCWGG 1 cut(s) 346
AlwI GGATC 1 cut(s) 571
AlwNI CAGNNNCTG 1 cut(s) 11
AoxI GGCC 1 cut(s) 384
ApaI GGGCCC 1 cut(s) 388
ApeKI GCWGC 1 cut(s) 11
ApoI RAATTY 1 cut(s) 598
AseI ATTAAT 1 cut(s) 285
AspS9I GGNCC 2 cut(s) 384, 385
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 1 cut(s) 472
BaeGI GKGCMC 1 cut(s) 388
BanII GRGCYC 1 cut(s) 388
BccI CCATC 1 cut(s) 509
BciT130I CCWGG 1 cut(s) 348
BcnI CCSGG 1 cut(s) 144
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 2 cut(s) 53, 628
BfmI CTRYAG 1 cut(s) 171
BfrI CTTAAG 1 cut(s) 358
BglI GCCNNNNNGGC 1 cut(s) 116
BisI GCNGC 2 cut(s) 12, 150
BlsI GCNGC 2 cut(s) 13, 151
Bme1390I CCNGG 2 cut(s) 144, 348
BmgT120I GGNCC 2 cut(s) 384, 385
BmiI GGNNCC 5 cut(s) 109, 148, 385, 386, 520
BmrFI CCNGG 2 cut(s) 144, 348
BmsI GCATC 2 cut(s) 372, 452
BpuEI CTTGAG 1 cut(s) 454
BpuMI CCSGG 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 347
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 380, 490
Bse1I ACTGG 2 cut(s) 226, 433
BseBI CCWGG 1 cut(s) 348
BseDI CCNNGG 1 cut(s) 347
BseGI GGATG 2 cut(s) 443, 520
BseLI CCNNNNNNNGG 3 cut(s) 232, 380, 490
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 2 cut(s) 226, 433
BseSI GKGCMC 1 cut(s) 388
BshFI GGCC 1 cut(s) 386
BsiSI CCGG 2 cut(s) 102, 144
BslFI GGGAC 1 cut(s) 262
BslI CCNNNNNNNGG 3 cut(s) 232, 380, 490
BsmFI GGGAC 1 cut(s) 262
BsnI GGCC 1 cut(s) 386
Bsp120I GGGCCC 1 cut(s) 384
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 1 cut(s) 563
BspACI CCGC 4 cut(s) 39, 105, 119, 150
BspANI GGCC 1 cut(s) 386
BspCNI CTCAG 1 cut(s) 127
BspLI GGNNCC 5 cut(s) 109, 148, 385, 386, 520
BspPI GGATC 1 cut(s) 571
BspTI CTTAAG 1 cut(s) 358
BsrI ACTGG 2 cut(s) 226, 433
BssECI CCNNGG 1 cut(s) 347
BssMI GATC 1 cut(s) 563
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 4 cut(s) 172, 205, 550, 585
BstAFI CTTAAG 1 cut(s) 358
BstDEI CTNAG 2 cut(s) 114, 308
BstENI CCTNNNNNAGG 1 cut(s) 488
BstF5I GGATG 2 cut(s) 443, 520
BstKTI GATC 1 cut(s) 566
BstMBI GATC 1 cut(s) 563
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNI CCWGG 1 cut(s) 348
BstSCI CCNGG 2 cut(s) 142, 346
BstSFI CTRYAG 1 cut(s) 171
BstSLI GKGCMC 1 cut(s) 388
BsuRI GGCC 1 cut(s) 386
BtsCI GGATG 2 cut(s) 443, 520
BtsIMutI CAGTG 1 cut(s) 590
CaiI CAGNNNCTG 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 384, 385
Csp6I GTAC 2 cut(s) 156, 572
CviJI RGCY 7 cut(s) 11, 62, 110, 149, 386, 521, 606
CviKI_1 RGCY 7 cut(s) 11, 62, 110, 149, 386, 521, 606
CviQI GTAC 2 cut(s) 156, 572
DdeI CTNAG 2 cut(s) 114, 308
DpnI GATC 1 cut(s) 565
DpnII GATC 1 cut(s) 563
EciI GGCGGA 1 cut(s) 120
Eco24I GRGCYC 1 cut(s) 388
EcoNI CCTNNNNNAGG 1 cut(s) 488
EcoO109I RGGNCCY 2 cut(s) 384, 385
EcoRII CCWGG 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 388
FaiI YATR 6 cut(s) 23, 266, 330, 488, 504, 623
FaqI GGGAC 1 cut(s) 262
FblI GTMKAC 2 cut(s) 333, 545
Fnu4HI GCNGC 2 cut(s) 12, 150
FokI GGATG 2 cut(s) 430, 527
FriOI GRGCYC 1 cut(s) 388
Fsp4HI GCNGC 2 cut(s) 12, 150
FspBI CTAG 2 cut(s) 53, 628
GluI GCNGC 2 cut(s) 12, 150
HaeIII GGCC 1 cut(s) 386
HapII CCGG 2 cut(s) 102, 144
HinfI GANTC 5 cut(s) 42, 250, 270, 343, 494
HpaII CCGG 2 cut(s) 102, 144
HphI GGTGA 1 cut(s) 472
Hpy166II GTNNAC 2 cut(s) 334, 546
Hpy188I TCNGA 1 cut(s) 47
Hpy188III TCNNGA 1 cut(s) 298
Hpy8I GTNNAC 2 cut(s) 334, 546
HpyAV CCTTC 2 cut(s) 235, 239
HpyCH4III ACNGT 4 cut(s) 172, 205, 550, 585
HpyCH4IV ACGT 2 cut(s) 321, 458
HpyCH4V TGCA 1 cut(s) 581
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 2 cut(s) 114, 308
HpySE526I ACGT 2 cut(s) 321, 458
Kzo9I GATC 1 cut(s) 563
LmnI GCTCC 3 cut(s) 107, 146, 518
LpnPI CCDG 9 cut(s) 101, 115, 157, 239, 333, 360, 414, 554, 588
LweI GCATC 2 cut(s) 372, 452
MaeI CTAG 2 cut(s) 53, 628
MaeII ACGT 2 cut(s) 321, 458
MaeIII GTNAC 3 cut(s) 129, 271, 478
MalI GATC 1 cut(s) 565
MboI GATC 1 cut(s) 563
MhlI GDGCHC 1 cut(s) 388
MluCI AATT 5 cut(s) 216, 395, 499, 530, 598
MlyI GAGTC 4 cut(s) 244, 279, 352, 488
MnlI CCTC 2 cut(s) 121, 484
MseI TTAA 3 cut(s) 285, 359, 455
MslI CAYNNNNRTG 1 cut(s) 485
MspCI CTTAAG 1 cut(s) 358
MspI CCGG 2 cut(s) 102, 144
MspR9I CCNGG 2 cut(s) 144, 348
MvaI CCWGG 1 cut(s) 348
MwoI GCNNNNNNNGC 1 cut(s) 116
NciI CCSGG 1 cut(s) 144
NdeII GATC 1 cut(s) 563
NlaIV GGNNCC 5 cut(s) 109, 148, 385, 386, 520
NmeAIII GCCGAG 1 cut(s) 547
NmuCI GTSAC 2 cut(s) 271, 478
PfeI GAWTC 1 cut(s) 42
PflMI CCANNNNNTGG 2 cut(s) 232, 380
PfoI TCCNGGA 1 cut(s) 142
PkrI GCNGC 2 cut(s) 13, 151
PleI GAGTC 4 cut(s) 244, 278, 351, 488
PpsI GAGTC 4 cut(s) 244, 278, 351, 488
PshBI ATTAAT 1 cut(s) 285
PsiI TTATAA 1 cut(s) 504
Psp1406I AACGTT 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 346
PspGI CCWGG 1 cut(s) 346
PspN4I GGNNCC 5 cut(s) 109, 148, 385, 386, 520
PspOMI GGGCCC 1 cut(s) 384
PspPI GGNCC 2 cut(s) 384, 385
PstNI CAGNNNCTG 1 cut(s) 11
RsaI GTAC 2 cut(s) 157, 573
RsaNI GTAC 2 cut(s) 156, 572
RseI CAYNNNNRTG 1 cut(s) 485
SaqAI TTAA 3 cut(s) 285, 359, 455
SatI GCNGC 2 cut(s) 12, 150
Sau3AI GATC 1 cut(s) 563
Sau96I GGNCC 2 cut(s) 384, 385
SchI GAGTC 4 cut(s) 244, 279, 352, 488
ScrFI CCNGG 2 cut(s) 144, 348
SduI GDGCHC 1 cut(s) 388
SetI ASST 3 cut(s) 324, 461, 573
SfaNI GCATC 2 cut(s) 372, 452
SfcI CTRYAG 1 cut(s) 171
SmiMI CAYNNNNRTG 1 cut(s) 485
SmlI CTYRAG 2 cut(s) 358, 469
SmoI CTYRAG 2 cut(s) 358, 469
SpeI ACTAGT 1 cut(s) 52
Sse9I AATT 5 cut(s) 216, 395, 499, 530, 598
SsiI CCGC 4 cut(s) 39, 105, 119, 150
SspMI CTAG 2 cut(s) 53, 628
StyD4I CCNGG 2 cut(s) 142, 346
TaaI ACNGT 4 cut(s) 172, 205, 550, 585
TaiI ACGT 2 cut(s) 324, 461
TasI AATT 5 cut(s) 216, 395, 499, 530, 598
TatI WGTACW 1 cut(s) 155
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 1 cut(s) 42
Tru1I TTAA 3 cut(s) 285, 359, 455
Tru9I TTAA 3 cut(s) 285, 359, 455
TscAI CASTG 1 cut(s) 590
TseFI GTSAC 2 cut(s) 271, 478
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 2 cut(s) 271, 478
TspDTI ATGAA 4 cut(s) 38, 47, 246, 522
TspGWI ACGGA 2 cut(s) 76, 127
TspRI CASTG 1 cut(s) 590
Van91I CCANNNNNTGG 2 cut(s) 232, 380
Vha464I CTTAAG 1 cut(s) 358
VspI ATTAAT 1 cut(s) 285
XagI CCTNNNNNAGG 1 cut(s) 488
XapI RAATTY 1 cut(s) 598
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XmiI GTMKAC 2 cut(s) 333, 545
XspI CTAG 2 cut(s) 53, 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.