Rroxscaffold_4G00305950

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0015760
Physical Location & Seq
Reverse (-)
26691641 .. 26709141
17501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00305950.1

Sequence Viewer

Length: 1065 bp
ATGAGCCAAAAAGTAGAATCTTACCTCGCAAGGTTTGGAACCCGAAGAACCCTAAAATCTCAATCTTCAAAATTCGAGCTCCACGGTGAAATCGTTGCAAGCCGCTTGAGGGAAGCATCTACGGCCTCGGGCTTCAAAAGCCCCCAAGAATCACCGGCTATGGAAAGTATTCAGCCCCAGCTGAAACTTCACGGCCATCCCTTTGCATCAATTTGTCTCCTAAACAAATATAATCTTGAGAATTCCATCATTAACGGATCGGATCGACCTAAATCCGTATTTGATCCGTTGAATAAATGGGTTAATGGATTCGGATCATTAATGGATCAACGGATCAAAATTTTCGATCAAAACCGGTCTAATAGTCACGGATCCGGAACTGGTTCGGATCAAAATCAGGAGTCACAGAAGTCATTAATACAAATCTCTTGGATAGAACTAAGTGCTGATGACGTAAATTATGTCTACCAAAGGAGTCCTGGGAAAATCTTTGAGGCCCTTACTCAAATTGGAACTGCTACAATCACAACAAAGAATACTAGTGAAGTGGAAGCATCCTATTGCTTAATGTCTGATTTCTCAAGTGATGTCACCCTTATGGAGGAGCAATACTCCATTATAAAGCCAAAAGAAACACTGCAATATCTCGATGAGGATTTGGACTGCCCTCCATCCTACAACAGCGTCACCGATTTGACGGCTGTCGACGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCTATCAAGGACCGGGTTCGCGACGAAACCCTAGCCATCCCCAAAGCAAAAAAACTGATCAATGCATCGCTGAAGCAGCAAAGGAAATTGAAGGATATGTCAATTTATGCCCCCACTCTTGTTCCTGAAAGAATGTCGATGTTGAATTTGGACACAAATAGATGTTTATTTCCAGAAAGCTCTCAGCAAAACACTGGTTCTGGGTCCTTCAAGCTCGAGGAGGATCCTGCCCCACCATCACCACCCAAGGAGAAAAAGGGTCGTTCCACGCCACCAATATTGTGGTTTGTAAGTGCTGAAGAGCTGGATTCCTTGTCATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

354

Amino Acids

39.47

Weight (kDa)

5.76

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAP2-GCS1 PF10699 136 - 214 5.8e-12 Male gamete fusion factor
SKA1 PF07160 227 - 354 1.6e-19 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 620
AccI GTMKAC 2 cut(s) 465, 705
AccII CGCG 1 cut(s) 762
AccIII TCCGGA 1 cut(s) 374
AciI CCGC 2 cut(s) 103, 711
AcoI YGGCCR 1 cut(s) 193
AcsI RAATTY 4 cut(s) 71, 241, 339, 886
AcuI CTGAAG 3 cut(s) 716, 833, 1059
AcyI GRCGYC 1 cut(s) 708
AfiI CCNNNNNNNGG 2 cut(s) 109, 601
AgeI ACCGGT 1 cut(s) 354
AgsI TTSAA 6 cut(s) 69, 136, 292, 832, 886, 952
AhlI ACTAGT 1 cut(s) 539
AjnI CCWGG 2 cut(s) 478, 738
AluBI AGCT 6 cut(s) 79, 181, 730, 921, 955, 1045
AluI AGCT 6 cut(s) 79, 181, 730, 921, 955, 1045
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 1 cut(s) 221
Ama87I CYCGRG 2 cut(s) 127, 956
Aor13HI TCCGGA 1 cut(s) 374
AoxI GGCC 4 cut(s) 123, 193, 495, 720
ApeKI GCWGC 1 cut(s) 817
ApoI RAATTY 4 cut(s) 71, 241, 339, 886
AseI ATTAAT 2 cut(s) 320, 416
AsiGI ACCGGT 1 cut(s) 354
Asp700I GAANNNNTTC 2 cut(s) 168, 382
AspS9I GGNCC 4 cut(s) 496, 736, 751, 945
AsuC2I CCSGG 1 cut(s) 755
AsuHPI GGTGA 5 cut(s) 98, 144, 583, 679, 972
AvaI CYCGRG 2 cut(s) 127, 956
AvaII GGWCC 3 cut(s) 736, 751, 945
BamHI GGATCC 2 cut(s) 371, 964
BanII GRGCYC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 1 cut(s) 829
BccI CCATC 5 cut(s) 204, 254, 679, 785, 985
BceAI ACGGC 4 cut(s) 138, 208, 698, 714
BciT130I CCWGG 2 cut(s) 480, 740
BclI TGATCA 1 cut(s) 798
BcnI CCSGG 1 cut(s) 755
BcoDI GTCTC 1 cut(s) 221
BcuI ACTAGT 1 cut(s) 539
BfaI CTAG 2 cut(s) 540, 773
BglI GCCNNNNNGGC 1 cut(s) 719
BisI GCNGC 3 cut(s) 103, 711, 818
BlsI GCNGC 3 cut(s) 104, 712, 819
Bme1390I CCNGG 3 cut(s) 480, 740, 755
Bme18I GGWCC 3 cut(s) 736, 751, 945
BmeT110I CYCGRG 2 cut(s) 127, 956
BmgT120I GGNCC 4 cut(s) 496, 736, 751, 945
BmiI GGNNCC 4 cut(s) 40, 373, 946, 966
BmrFI CCNGG 3 cut(s) 480, 740, 755
BmsI GCATC 4 cut(s) 125, 215, 563, 815
BoxI GACNNNNGTC 1 cut(s) 701
BplI GAGNNNNNCTC 2 cut(s) 596, 628
BpuEI CTTGAG 3 cut(s) 127, 257, 565
BpuMI CCSGG 1 cut(s) 755
BsaBI GATNNNNATC 2 cut(s) 313, 393
BsaHI GRCGYC 1 cut(s) 708
BsaJI CCNNGG 5 cut(s) 82, 126, 479, 723, 987
BsaWI WCCGGW 2 cut(s) 354, 374
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 601
Bse118I RCCGGY 2 cut(s) 154, 354
Bse1I ACTGG 2 cut(s) 385, 940
Bse8I GATNNNNATC 2 cut(s) 313, 393
BseAI TCCGGA 1 cut(s) 374
BseBI CCWGG 2 cut(s) 480, 740
BseDI CCNNGG 5 cut(s) 82, 126, 479, 723, 987
BseGI GGATG 4 cut(s) 196, 554, 671, 777
BseJI GATNNNNATC 2 cut(s) 313, 393
BseLI CCNNNNNNNGG 2 cut(s) 109, 601
BseMII CTCAG 1 cut(s) 938
BseNI ACTGG 2 cut(s) 385, 940
BseRI GAGGAG 2 cut(s) 617, 974
BseXI GCAGC 1 cut(s) 829
BseYI CCCAGC 1 cut(s) 177
Bsh1236I CGCG 1 cut(s) 762
BshFI GGCC 4 cut(s) 125, 195, 497, 722
BshTI ACCGGT 1 cut(s) 354
BsiHKAI GWGCWC 1 cut(s) 81
BsiHKCI CYCGRG 2 cut(s) 127, 956
BsiSI CCGG 4 cut(s) 155, 355, 375, 754
BslI CCNNNNNNNGG 2 cut(s) 109, 601
BsmAI GTCTC 1 cut(s) 221
BsnI GGCC 4 cut(s) 125, 195, 497, 722
BsoBI CYCGRG 2 cut(s) 127, 956
Bsp1286I GDGCHC 1 cut(s) 81
Bsp13I TCCGGA 1 cut(s) 374
Bsp19I CCATGG 1 cut(s) 723
Bsp68I TCGCGA 1 cut(s) 762
BspACI CCGC 2 cut(s) 103, 711
BspANI GGCC 4 cut(s) 125, 195, 497, 722
BspCNI CTCAG 1 cut(s) 937
BspEI TCCGGA 1 cut(s) 374
BspFNI CGCG 1 cut(s) 762
BspLI GGNNCC 4 cut(s) 40, 373, 946, 966
BspQI GCTCTTC 1 cut(s) 1035
BsrFI RCCGGY 2 cut(s) 154, 354
BsrI ACTGG 2 cut(s) 385, 940
BssAI RCCGGY 2 cut(s) 154, 354
BssECI CCNNGG 5 cut(s) 82, 126, 479, 723, 987
BssNI GRCGYC 1 cut(s) 708
BssT1I CCWWGG 2 cut(s) 723, 987
Bst2UI CCWGG 2 cut(s) 480, 740
Bst4CI ACNGT 1 cut(s) 86
Bst6I CTCTTC 1 cut(s) 1035
BstACI GRCGYC 1 cut(s) 708
BstC8I GCNNGC 1 cut(s) 100
BstDEI CTNAG 2 cut(s) 440, 924
BstDSI CCRYGG 2 cut(s) 82, 723
BstENI CCTNNNNNAGG 1 cut(s) 599
BstF5I GGATG 4 cut(s) 196, 554, 671, 777
BstFNI CGCG 1 cut(s) 762
BstMAI GTCTC 1 cut(s) 221
BstMWI GCNNNNNNNGC 5 cut(s) 122, 138, 707, 719, 817
BstNI CCWGG 2 cut(s) 480, 740
BstPAI GACNNNNGTC 1 cut(s) 701
BstSCI CCNGG 3 cut(s) 478, 738, 753
BstUI CGCG 1 cut(s) 762
BstV1I GCAGC 1 cut(s) 829
BstX2I RGATCY 2 cut(s) 371, 964
BstXI CCANNNNNNTGG 1 cut(s) 1023
BstYI RGATCY 2 cut(s) 371, 964
BsuRI GGCC 4 cut(s) 125, 195, 497, 722
BtgI CCRYGG 2 cut(s) 82, 723
BtgZI GCGATG 1 cut(s) 792
BtsCI GGATG 4 cut(s) 196, 554, 671, 777
BtsI GCAGTG 1 cut(s) 635
BtsIMutI CAGTG 2 cut(s) 635, 933
BtuMI TCGCGA 1 cut(s) 762
Cac8I GCNNGC 1 cut(s) 100
Cfr10I RCCGGY 2 cut(s) 154, 354
Cfr13I GGNCC 4 cut(s) 496, 736, 751, 945
CseI GACGC 2 cut(s) 673, 716
CspAI ACCGGT 1 cut(s) 354
CspCI CAANNNNNGTGG 2 cut(s) 1002, 1037
CviAII CATG 1 cut(s) 724
DdeI CTNAG 2 cut(s) 440, 924
EaeI YGGCCR 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 1035
EarI CTCTTC 1 cut(s) 1035
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 2 cut(s) 723, 987
Eco24I GRGCYC 1 cut(s) 81
Eco47I GGWCC 3 cut(s) 736, 751, 945
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 3 cut(s) 716, 833, 1059
Eco88I CYCGRG 2 cut(s) 127, 956
EcoICRI GAGCTC 1 cut(s) 79
EcoNI CCTNNNNNAGG 1 cut(s) 599
EcoO109I RGGNCCY 2 cut(s) 496, 945
EcoRI GAATTC 1 cut(s) 241
EcoRII CCWGG 2 cut(s) 478, 738
EcoT14I CCWWGG 2 cut(s) 723, 987
EcoT22I ATGCAT 1 cut(s) 808
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 2 cut(s) 723, 987
FaeI CATG 1 cut(s) 727
FaiI YATR 8 cut(s) 161, 231, 462, 599, 620, 725, 839, 849
FatI CATG 1 cut(s) 723
FbaI TGATCA 1 cut(s) 798
FblI GTMKAC 2 cut(s) 465, 705
Fnu4HI GCNGC 3 cut(s) 103, 711, 818
FokI GGATG 4 cut(s) 183, 541, 658, 764
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 3 cut(s) 103, 711, 818
FspBI CTAG 2 cut(s) 540, 773
GluI GCNGC 3 cut(s) 103, 711, 818
GsaI CCCAGC 1 cut(s) 181
HaeIII GGCC 4 cut(s) 125, 195, 497, 722
HapII CCGG 4 cut(s) 155, 355, 375, 754
HgaI GACGC 2 cut(s) 673, 716
Hin1I GRCGYC 1 cut(s) 708
Hin1II CATG 1 cut(s) 727
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HinfI GANTC 6 cut(s) 17, 149, 309, 401, 475, 1049
HpaII CCGG 4 cut(s) 155, 355, 375, 754
HphI GGTGA 5 cut(s) 98, 144, 583, 679, 972
Hpy166II GTNNAC 2 cut(s) 466, 706
Hpy188I TCNGA 4 cut(s) 262, 314, 388, 574
Hpy188III TCNNGA 8 cut(s) 236, 375, 398, 647, 761, 866, 914, 1062
Hpy8I GTNNAC 2 cut(s) 466, 706
Hpy99I CGWCG 2 cut(s) 710, 767
HpyAV CCTTC 3 cut(s) 712, 826, 958
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 4 cut(s) 98, 206, 640, 806
HpyF10VI GCNNNNNNNGC 5 cut(s) 122, 138, 707, 719, 817
HpyF3I CTNAG 2 cut(s) 440, 924
HpySE526I ACGT 1 cut(s) 453
Hsp92I GRCGYC 1 cut(s) 708
Hsp92II CATG 1 cut(s) 727
Kpn2I TCCGGA 1 cut(s) 374
Ksp22I TGATCA 1 cut(s) 798
LguI GCTCTTC 1 cut(s) 1035
LmnI GCTCC 3 cut(s) 84, 604, 727
Lsp1109I GCAGC 1 cut(s) 829
LweI GCATC 4 cut(s) 125, 215, 563, 815
MaeI CTAG 2 cut(s) 540, 773
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 4 cut(s) 365, 402, 589, 685
MboII GAAGA 3 cut(s) 57, 57, 1052
MflI RGATCY 2 cut(s) 371, 964
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 9 cut(s) 71, 210, 241, 339, 457, 507, 827, 843, 886
MlyI GAGTC 2 cut(s) 410, 484
MnlI CCTC 9 cut(s) 35, 102, 136, 487, 595, 646, 678, 952, 955
Mph1103I ATGCAT 1 cut(s) 808
MroI TCCGGA 1 cut(s) 374
MroXI GAANNNNTTC 2 cut(s) 168, 382
MseI TTAA 5 cut(s) 252, 303, 320, 416, 566
MslI CAYNNNNRTG 1 cut(s) 596
MspA1I CMGCKG 1 cut(s) 181
MspI CCGG 4 cut(s) 155, 355, 375, 754
MspR9I CCNGG 3 cut(s) 480, 740, 755
MvaI CCWGG 2 cut(s) 480, 740
MvnI CGCG 1 cut(s) 762
MwoI GCNNNNNNNGC 5 cut(s) 122, 138, 707, 719, 817
NciI CCSGG 1 cut(s) 755
NcoI CCATGG 1 cut(s) 723
NlaIII CATG 1 cut(s) 727
NlaIV GGNNCC 4 cut(s) 40, 373, 946, 966
NmuCI GTSAC 4 cut(s) 365, 402, 589, 685
NruI TCGCGA 1 cut(s) 762
NsiI ATGCAT 1 cut(s) 808
PaeR7I CTCGAG 1 cut(s) 956
PciSI GCTCTTC 1 cut(s) 1035
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 711
PdmI GAANNNNTTC 2 cut(s) 168, 382
PfeI GAWTC 4 cut(s) 17, 149, 309, 1049
PinAI ACCGGT 1 cut(s) 354
PkrI GCNGC 3 cut(s) 104, 712, 819
PleI GAGTC 2 cut(s) 409, 483
PpsI GAGTC 2 cut(s) 409, 483
PpuMI RGGWCCY 1 cut(s) 945
PshAI GACNNNNGTC 1 cut(s) 701
PshBI ATTAAT 2 cut(s) 320, 416
PsiI TTATAA 1 cut(s) 620
Psp124BI GAGCTC 1 cut(s) 81
Psp5II RGGWCCY 1 cut(s) 945
Psp6I CCWGG 2 cut(s) 478, 738
PspFI CCCAGC 1 cut(s) 177
PspGI CCWGG 2 cut(s) 478, 738
PspN4I GGNNCC 4 cut(s) 40, 373, 946, 966
PspPI GGNCC 4 cut(s) 496, 736, 751, 945
PspPPI RGGWCCY 1 cut(s) 945
PspXI VCTCGAGB 1 cut(s) 956
PsuI RGATCY 2 cut(s) 371, 964
PvuII CAGCTG 1 cut(s) 181
RruI TCGCGA 1 cut(s) 762
RseI CAYNNNNRTG 1 cut(s) 596
SacI GAGCTC 1 cut(s) 81
SalI GTCGAC 1 cut(s) 704
SapI GCTCTTC 1 cut(s) 1035
SaqAI TTAA 5 cut(s) 252, 303, 320, 416, 566
SatI GCNGC 3 cut(s) 103, 711, 818
Sau96I GGNCC 4 cut(s) 496, 736, 751, 945
SchI GAGTC 2 cut(s) 410, 484
ScrFI CCNGG 3 cut(s) 480, 740, 755
SduI GDGCHC 1 cut(s) 81
SfaNI GCATC 4 cut(s) 125, 215, 563, 815
Sfr274I CTCGAG 1 cut(s) 956
SinI GGWCC 3 cut(s) 736, 751, 945
SlaI CTCGAG 1 cut(s) 956
SmiMI CAYNNNNRTG 1 cut(s) 596
SmlI CTYRAG 4 cut(s) 106, 236, 580, 956
SmoI CTYRAG 4 cut(s) 106, 236, 580, 956
SpeI ACTAGT 1 cut(s) 539
Sse9I AATT 9 cut(s) 71, 210, 241, 339, 457, 507, 827, 843, 886
SsiI CCGC 2 cut(s) 103, 711
SspI AATATT 1 cut(s) 1020
SspMI CTAG 2 cut(s) 540, 773
SstI GAGCTC 1 cut(s) 81
StyD4I CCNGG 3 cut(s) 478, 738, 753
StyI CCWWGG 2 cut(s) 723, 987
TaaI ACNGT 1 cut(s) 86
TaiI ACGT 1 cut(s) 456
TaqI TCGA 7 cut(s) 75, 265, 345, 648, 705, 878, 957
TasI AATT 9 cut(s) 71, 210, 241, 339, 457, 507, 827, 843, 886
TauI GCSGC 2 cut(s) 105, 713
TfiI GAWTC 4 cut(s) 17, 149, 309, 1049
Tru1I TTAA 5 cut(s) 252, 303, 320, 416, 566
Tru9I TTAA 5 cut(s) 252, 303, 320, 416, 566
TscAI CASTG 2 cut(s) 642, 940
TseFI GTSAC 4 cut(s) 365, 402, 589, 685
TseI GCWGC 1 cut(s) 817
Tsp45I GTSAC 4 cut(s) 365, 402, 589, 685
TspGWI ACGGA 5 cut(s) 265, 270, 276, 346, 384
TspRI CASTG 2 cut(s) 642, 940
VpaK11BI GGWCC 3 cut(s) 736, 751, 945
VspI ATTAAT 2 cut(s) 320, 416
XagI CCTNNNNNAGG 1 cut(s) 599
XapI RAATTY 4 cut(s) 71, 241, 339, 886
XcmI CCANNNNNNNNNTGG 2 cut(s) 476, 1020
XhoI CTCGAG 1 cut(s) 956
XmiI GTMKAC 2 cut(s) 465, 705
XmnI GAANNNNTTC 2 cut(s) 168, 382
XspI CTAG 2 cut(s) 540, 773
Zsp2I ATGCAT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.