Rroxscaffold_5G00353480

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015760
Physical Location & Seq
Forward (+)
31279239 .. 31280239
1001 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_5G00353480.1

Sequence Viewer

Length: 453 bp
ATGAAAGGAAAAAAATACCGCAAGTTTCACAATCTGTGGAACCATAACACGGCCGAGTGTGTCAAGTCGAAGGATCATATTCAGGTATGGCTCAATAATGGTAGTCTACAAGTTGAATCTCCCAAAACCGCGGCGGCGTTAGTGGATTTGAATCCCTTCCTTAATACCGGAGTTGATATGACTTGGAATAGAAAAGGCCATAGGAAGCTAACTCTCGATTTGATTACCGAGAGGGAAAAGGATAAAGAGGAAGAGGTTCTAGAAAGGAAGAGACCTAAGCATATCGGCGAGGCCTCACCAAAGTGGGTCCCGTGCTCGCGATGCAAAGAGGAATGTGGCATCCAAGTGTCAAATGAGGAGGTCGAAGAAAATCAAGGGGAGGACATTAAGGATAAAGCCATGGAGAGATTTAAGAGAGAAAAGGTCCAAAATGCGTCCGGATACATTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

150

Amino Acids

17.45

Weight (kDa)

8.62

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 106
AccII CGCG 2 cut(s) 131, 319
AccIII TCCGGA 1 cut(s) 437
AciI CCGC 4 cut(s) 19, 129, 131, 134
AclWI GGATC 1 cut(s) 81
AcoI YGGCCR 1 cut(s) 51
AfiI CCNNNNNNNGG 1 cut(s) 49
AgsI TTSAA 2 cut(s) 116, 151
AleI CACNNNNGTG 1 cut(s) 301
AluBI AGCT 1 cut(s) 208
AluI AGCT 1 cut(s) 208
Alw21I GWGCWC 1 cut(s) 317
Alw26I GTCTC 1 cut(s) 265
AlwI GGATC 1 cut(s) 81
Aor13HI TCCGGA 1 cut(s) 437
AoxI GGCC 3 cut(s) 51, 196, 291
Asp700I GAANNNNTTC 2 cut(s) 155, 255
AspS9I GGNCC 2 cut(s) 307, 424
AsuHPI GGTGA 1 cut(s) 288
AvaII GGWCC 2 cut(s) 307, 424
Bbv12I GWGCWC 1 cut(s) 317
BceAI ACGGC 1 cut(s) 66
BciVI GTATCC 1 cut(s) 434
BcoDI GTCTC 1 cut(s) 265
BfaI CTAG 2 cut(s) 260, 451
BfuI GTATCC 1 cut(s) 434
BisI GCNGC 2 cut(s) 132, 135
BlsI GCNGC 2 cut(s) 133, 136
Bme18I GGWCC 2 cut(s) 307, 424
BmgT120I GGNCC 2 cut(s) 307, 424
BmiI GGNNCC 3 cut(s) 41, 308, 309
BmsI GCATC 2 cut(s) 311, 348
Bpu10I CCTNAGC 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 265
BsaJI CCNNGG 2 cut(s) 129, 399
BsaWI WCCGGW 2 cut(s) 167, 437
Bsc4I CCNNNNNNNGG 1 cut(s) 49
Bse8I GATNNNNATC 1 cut(s) 150
BseAI TCCGGA 1 cut(s) 437
BseDI CCNNGG 2 cut(s) 129, 399
BseGI GGATG 1 cut(s) 339
BseJI GATNNNNATC 1 cut(s) 150
BseLI CCNNNNNNNGG 1 cut(s) 49
BseRI GAGGAG 1 cut(s) 371
BseX3I CGGCCG 1 cut(s) 51
Bsh1236I CGCG 2 cut(s) 131, 319
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 3 cut(s) 53, 198, 293
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 317
BsiSI CCGG 2 cut(s) 168, 438
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 1 cut(s) 49
BsmAI GTCTC 1 cut(s) 265
BsmFI GGGAC 1 cut(s) 293
BsnI GGCC 3 cut(s) 53, 198, 293
Bso31I GGTCTC 1 cut(s) 265
Bsp1286I GDGCHC 1 cut(s) 317
Bsp13I TCCGGA 1 cut(s) 437
Bsp143I GATC 1 cut(s) 73
Bsp19I CCATGG 1 cut(s) 399
Bsp68I TCGCGA 1 cut(s) 319
BspACI CCGC 4 cut(s) 19, 129, 131, 134
BspANI GGCC 3 cut(s) 53, 198, 293
BspEI TCCGGA 1 cut(s) 437
BspFNI CGCG 2 cut(s) 131, 319
BspLI GGNNCC 3 cut(s) 41, 308, 309
BspPI GGATC 1 cut(s) 81
BspTNI GGTCTC 1 cut(s) 265
BssECI CCNNGG 2 cut(s) 129, 399
BssMI GATC 1 cut(s) 73
BssT1I CCWWGG 1 cut(s) 399
Bst6I CTCTTC 2 cut(s) 246, 263
BstC8I GCNNGC 1 cut(s) 317
BstDEI CTNAG 1 cut(s) 276
BstDSI CCRYGG 2 cut(s) 129, 399
BstF5I GGATG 1 cut(s) 339
BstFNI CGCG 2 cut(s) 131, 319
BstKTI GATC 1 cut(s) 76
BstMAI GTCTC 1 cut(s) 265
BstMBI GATC 1 cut(s) 73
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 1 cut(s) 321
BstUI CGCG 2 cut(s) 131, 319
BstZI CGGCCG 1 cut(s) 51
BsuI GTATCC 1 cut(s) 434
BsuRI GGCC 3 cut(s) 53, 198, 293
BtgI CCRYGG 2 cut(s) 129, 399
BtgZI GCGATG 1 cut(s) 334
BtsCI GGATG 1 cut(s) 339
BtuMI TCGCGA 1 cut(s) 319
Cac8I GCNNGC 1 cut(s) 317
Cfr13I GGNCC 2 cut(s) 307, 424
Cfr42I CCGCGG 1 cut(s) 132
CseI GACGC 1 cut(s) 423
CviAII CATG 1 cut(s) 400
CviJI RGCY 6 cut(s) 53, 91, 198, 208, 293, 398
CviKI_1 RGCY 6 cut(s) 53, 91, 198, 208, 293, 398
DdeI CTNAG 1 cut(s) 276
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 51
EagI CGGCCG 1 cut(s) 51
Eam1104I CTCTTC 2 cut(s) 246, 263
EarI CTCTTC 2 cut(s) 246, 263
EclXI CGGCCG 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 399
Eco147I AGGCCT 1 cut(s) 293
Eco31I GGTCTC 1 cut(s) 265
Eco47I GGWCC 2 cut(s) 307, 424
Eco52I CGGCCG 1 cut(s) 51
EcoO109I RGGNCCY 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 399
FaeI CATG 1 cut(s) 403
FaiI YATR 7 cut(s) 45, 78, 88, 179, 201, 282, 401
FaqI GGGAC 1 cut(s) 293
FatI CATG 1 cut(s) 399
FblI GTMKAC 1 cut(s) 106
Fnu4HI GCNGC 2 cut(s) 132, 135
FokI GGATG 1 cut(s) 326
Fsp4HI GCNGC 2 cut(s) 132, 135
FspBI CTAG 2 cut(s) 260, 451
GluI GCNGC 2 cut(s) 132, 135
HaeIII GGCC 3 cut(s) 53, 198, 293
HapII CCGG 2 cut(s) 168, 438
HgaI GACGC 1 cut(s) 423
Hin1II CATG 1 cut(s) 403
HinfI GANTC 2 cut(s) 116, 151
HpaII CCGG 2 cut(s) 168, 438
HphI GGTGA 1 cut(s) 288
Hpy166II GTNNAC 1 cut(s) 107
Hpy188III TCNNGA 4 cut(s) 215, 260, 318, 438
Hpy8I GTNNAC 1 cut(s) 107
HpyAV CCTTC 2 cut(s) 64, 166
HpyCH4V TGCA 1 cut(s) 324
HpyF10VI GCNNNNNNNGC 1 cut(s) 321
HpyF3I CTNAG 1 cut(s) 276
Hsp92II CATG 1 cut(s) 403
KflI GGGWCCC 1 cut(s) 307
Kpn2I TCCGGA 1 cut(s) 437
KspI CCGCGG 1 cut(s) 132
Kzo9I GATC 1 cut(s) 73
LpnPI CCDG 2 cut(s) 68, 181
LweI GCATC 2 cut(s) 311, 348
MaeI CTAG 2 cut(s) 260, 451
MalI GATC 1 cut(s) 75
MboI GATC 1 cut(s) 73
MboII GAAGA 3 cut(s) 263, 280, 377
MhlI GDGCHC 1 cut(s) 317
MnlI CCTC 9 cut(s) 225, 241, 247, 283, 304, 322, 349, 352, 373
MroI TCCGGA 1 cut(s) 437
MroXI GAANNNNTTC 2 cut(s) 155, 255
MseI TTAA 3 cut(s) 162, 387, 411
MslI CAYNNNNRTG 2 cut(s) 301, 344
MspA1I CMGCKG 1 cut(s) 131
MspI CCGG 2 cut(s) 168, 438
MvnI CGCG 2 cut(s) 131, 319
MwoI GCNNNNNNNGC 1 cut(s) 321
NcoI CCATGG 1 cut(s) 399
NdeII GATC 1 cut(s) 73
NlaIII CATG 1 cut(s) 403
NlaIV GGNNCC 3 cut(s) 41, 308, 309
NmeAIII GCCGAG 1 cut(s) 79
NruI TCGCGA 1 cut(s) 319
OliI CACNNNNGTG 1 cut(s) 301
PceI AGGCCT 1 cut(s) 293
PdmI GAANNNNTTC 2 cut(s) 155, 255
PfeI GAWTC 2 cut(s) 116, 151
PkrI GCNGC 2 cut(s) 133, 136
PpuMI RGGWCCY 1 cut(s) 307
Psp5II RGGWCCY 1 cut(s) 307
PspN4I GGNNCC 3 cut(s) 41, 308, 309
PspPI GGNCC 2 cut(s) 307, 424
PspPPI RGGWCCY 1 cut(s) 307
RruI TCGCGA 1 cut(s) 319
RseI CAYNNNNRTG 2 cut(s) 301, 344
SacII CCGCGG 1 cut(s) 132
SaqAI TTAA 3 cut(s) 162, 387, 411
SatI GCNGC 2 cut(s) 132, 135
Sau3AI GATC 1 cut(s) 73
Sau96I GGNCC 2 cut(s) 307, 424
SduI GDGCHC 1 cut(s) 317
SetI ASST 6 cut(s) 87, 210, 258, 277, 363, 426
SfaNI GCATC 2 cut(s) 311, 348
Sfr303I CCGCGG 1 cut(s) 132
SgrBI CCGCGG 1 cut(s) 132
SinI GGWCC 2 cut(s) 307, 424
SmiMI CAYNNNNRTG 2 cut(s) 301, 344
SseBI AGGCCT 1 cut(s) 293
SsiI CCGC 4 cut(s) 19, 129, 131, 134
SspMI CTAG 2 cut(s) 260, 451
StuI AGGCCT 1 cut(s) 293
StyI CCWWGG 1 cut(s) 399
TaqI TCGA 3 cut(s) 68, 216, 363
TauI GCSGC 2 cut(s) 134, 137
TfiI GAWTC 2 cut(s) 116, 151
Tru1I TTAA 3 cut(s) 162, 387, 411
Tru9I TTAA 3 cut(s) 162, 387, 411
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 2 cut(s) 307, 424
XbaI TCTAGA 1 cut(s) 259
XmiI GTMKAC 1 cut(s) 106
XmnI GAANNNNTTC 2 cut(s) 155, 255
XspI CTAG 2 cut(s) 260, 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.