RLG00000035889

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016428
Physical Location & Seq
Reverse (-)
73137144 .. 73138930
1787 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035889

Sequence Viewer

Length: 681 bp
ATGACGCCATGGGAGCATCGCCATGAACCTGAAGAGCAGGAACTGCAGGAACGACATAGAGTGCTACGCTTGGAGAAATTGTGGAGAATGGGCAAGGAGTTGGATGGCCCGGGTTCGGAAGTGCTGTCTACGCTTTTGGAACAACAAACTAAAGGGCTATGCACGAGAATGAGAGTCTACGCAGAAGAATTTGAGGGAGAGCACCGCCATGAGACCACCGGAGATGCGACTCTTCTGGCTGTATGCAACAAATCAAGCCATTTCGATGTTGCTAAGGCGGGAGAGTATGGAAGCTGTGACGTCCTCCGAGAGCTCCAGCCAGTTATAGTAGGTTCGAGTTCGGCGCCGGAGGAGGCGATGATGCTGGTGGAGGTGGTGTTGTTGGCGGCAGTGGTGATGATGGTGGTGCTCCTAGCCATTGTTCCAATGGTGGTTAGGGTTGTTTTGGGCCCCCGGGTTGTCTTCAACTCTAGTGGTGGTGCTATTGGCTATGGCGGCATTACCGGCTGGGGCAGCGGCAACGCAGACGGTTTTGGTAAAGCCGGTGGTGTCGGCTCTATTGCTAACGGAGGCAGCGCTAAAGTCAGTCTGCTTCTCGGGTGGCTAGGGTTTGTTTCATGCCCTAGGATTATCTTTGGGATTGCATGTGGGCTTAGTAGCCCACTTTTGTTTTTATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

24.24

Weight (kDa)

5.55

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 303
AccB1I GGYRCC 1 cut(s) 343
AccI GTMKAC 2 cut(s) 128, 177
AciI CCGC 5 cut(s) 205, 278, 386, 495, 516
AcsI RAATTY 1 cut(s) 188
AcuI CTGAAG 1 cut(s) 51
AcyI GRCGYC 3 cut(s) 5, 300, 344
AfeI AGCGCT 1 cut(s) 577
AfiI CCNNNNNNNGG 1 cut(s) 115
AgsI TTSAA 1 cut(s) 466
AluBI AGCT 2 cut(s) 294, 313
AluI AGCT 2 cut(s) 294, 313
Alw21I GWGCWC 3 cut(s) 204, 315, 411
Alw26I GTCTC 1 cut(s) 206
AlwNI CAGNNNCTG 1 cut(s) 43
Ama87I CYCGRG 3 cut(s) 109, 453, 596
Aor51HI AGCGCT 1 cut(s) 577
AoxI GGCC 2 cut(s) 106, 448
ApaI GGGCCC 1 cut(s) 452
ApeKI GCWGC 2 cut(s) 513, 573
ApoI RAATTY 1 cut(s) 188
AspA2I CCTAGG 1 cut(s) 623
AspLEI GCGC 2 cut(s) 346, 578
AspS9I GGNCC 3 cut(s) 107, 448, 449
AsuC2I CCSGG 4 cut(s) 110, 111, 454, 455
AsuHPI GGTGA 1 cut(s) 406
AvaI CYCGRG 3 cut(s) 109, 453, 596
AvrII CCTAGG 1 cut(s) 623
BaeGI GKGCMC 1 cut(s) 452
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 2 cut(s) 315, 452
BauI CACGAG 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 454
Bbv12I GWGCWC 3 cut(s) 204, 315, 411
BbvI GCAGC 2 cut(s) 525, 585
BccI CCATC 2 cut(s) 98, 394
BcnI CCSGG 4 cut(s) 110, 111, 454, 455
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 4 cut(s) 413, 471, 605, 624
BfmI CTRYAG 1 cut(s) 44
BfoI RGCGCY 2 cut(s) 347, 579
BisI GCNGC 5 cut(s) 387, 496, 514, 517, 574
BlnI CCTAGG 1 cut(s) 623
BlsI GCNGC 5 cut(s) 388, 497, 515, 518, 575
Bme1390I CCNGG 4 cut(s) 110, 111, 454, 455
BmeT110I CYCGRG 3 cut(s) 109, 453, 596
BmgT120I GGNCC 3 cut(s) 107, 448, 449
BmiI GGNNCC 3 cut(s) 345, 450, 451
BmrFI CCNGG 4 cut(s) 110, 111, 454, 455
BmsI GCATC 3 cut(s) 25, 214, 351
BpiI GAAGAC 1 cut(s) 454
BpmI CTGGAG 1 cut(s) 299
Bpu10I CCTNAGC 1 cut(s) 273
BpuMI CCSGG 4 cut(s) 110, 111, 454, 455
BsaHI GRCGYC 3 cut(s) 5, 300, 344
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 5 cut(s) 8, 109, 452, 453, 623
BsaWI WCCGGW 1 cut(s) 218
BsaXI ACNNNNNCTCC 4 cut(s) 65, 95, 362, 392
Bsc4I CCNNNNNNNGG 1 cut(s) 115
Bse118I RCCGGY 2 cut(s) 503, 542
Bse1I ACTGG 1 cut(s) 320
BseDI CCNNGG 5 cut(s) 8, 109, 452, 453, 623
BseGI GGATG 1 cut(s) 109
BseLI CCNNNNNNNGG 1 cut(s) 115
BseNI ACTGG 1 cut(s) 320
BseRI GAGGAG 1 cut(s) 365
BseSI GKGCMC 1 cut(s) 452
BseXI GCAGC 2 cut(s) 525, 585
BseYI CCCAGC 1 cut(s) 507
BshFI GGCC 2 cut(s) 108, 450
BshNI GGYRCC 1 cut(s) 343
BsiHKAI GWGCWC 3 cut(s) 204, 315, 411
BsiHKCI CYCGRG 3 cut(s) 109, 453, 596
BsiSI CCGG 6 cut(s) 110, 219, 347, 454, 504, 543
BslI CCNNNNNNNGG 1 cut(s) 115
BsmAI GTCTC 1 cut(s) 206
BsnI GGCC 2 cut(s) 108, 450
Bso31I GGTCTC 1 cut(s) 206
BsoBI CYCGRG 3 cut(s) 109, 453, 596
Bsp120I GGGCCC 1 cut(s) 448
Bsp1286I GDGCHC 4 cut(s) 204, 315, 411, 452
Bsp19I CCATGG 1 cut(s) 8
BspACI CCGC 5 cut(s) 205, 278, 386, 495, 516
BspANI GGCC 2 cut(s) 108, 450
BspLI GGNNCC 3 cut(s) 345, 450, 451
BspMAI CTGCAG 1 cut(s) 48
BspQI GCTCTTC 1 cut(s) 27
BspT107I GGYRCC 1 cut(s) 343
BspTNI GGTCTC 1 cut(s) 206
BsrFI RCCGGY 2 cut(s) 503, 542
BsrI ACTGG 1 cut(s) 320
BssAI RCCGGY 2 cut(s) 503, 542
BssECI CCNNGG 5 cut(s) 8, 109, 452, 453, 623
BssNI GRCGYC 3 cut(s) 5, 300, 344
BssSI CACGAG 1 cut(s) 163
BssT1I CCWWGG 2 cut(s) 8, 623
Bst2BI CACGAG 1 cut(s) 163
Bst4CI ACNGT 1 cut(s) 530
Bst6I CTCTTC 2 cut(s) 27, 237
BstACI GRCGYC 3 cut(s) 5, 300, 344
BstAPI GCANNNNNTGC 1 cut(s) 43
BstDEI CTNAG 2 cut(s) 273, 653
BstDSI CCRYGG 1 cut(s) 8
BstF5I GGATG 1 cut(s) 109
BstH2I RGCGCY 2 cut(s) 347, 579
BstHHI GCGC 2 cut(s) 346, 578
BstMAI GTCTC 1 cut(s) 206
BstMWI GCNNNNNNNGC 6 cut(s) 13, 43, 130, 495, 504, 513
BstNSI RCATGY 1 cut(s) 648
BstSCI CCNGG 4 cut(s) 108, 109, 452, 453
BstSFI CTRYAG 1 cut(s) 44
BstSLI GKGCMC 1 cut(s) 452
BstV1I GCAGC 2 cut(s) 525, 585
BstV2I GAAGAC 1 cut(s) 454
BsuRI GGCC 2 cut(s) 108, 450
BtgI CCRYGG 1 cut(s) 8
BtgZI GCGATG 2 cut(s) 2, 371
BtsCI GGATG 1 cut(s) 109
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 1 cut(s) 396
CaiI CAGNNNCTG 1 cut(s) 43
CfoI GCGC 2 cut(s) 346, 578
Cfr10I RCCGGY 2 cut(s) 503, 542
Cfr13I GGNCC 3 cut(s) 107, 448, 449
Cfr9I CCCGGG 2 cut(s) 109, 453
CseI GACGC 1 cut(s) 13
CspCI CAANNNNNGTGG 2 cut(s) 454, 489
CviAII CATG 5 cut(s) 9, 23, 209, 618, 645
DdeI CTNAG 2 cut(s) 273, 653
DinI GGCGCC 1 cut(s) 345
Eam1104I CTCTTC 2 cut(s) 27, 237
EarI CTCTTC 2 cut(s) 27, 237
Ecl136II GAGCTC 1 cut(s) 313
Eco130I CCWWGG 2 cut(s) 8, 623
Eco24I GRGCYC 2 cut(s) 315, 452
Eco31I GGTCTC 1 cut(s) 206
Eco47III AGCGCT 1 cut(s) 577
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 51
Eco88I CYCGRG 3 cut(s) 109, 453, 596
EcoICRI GAGCTC 1 cut(s) 313
EcoO109I RGGNCCY 1 cut(s) 449
EcoT14I CCWWGG 2 cut(s) 8, 623
EcoT38I GRGCYC 2 cut(s) 315, 452
EgeI GGCGCC 1 cut(s) 345
EheI GGCGCC 1 cut(s) 345
ErhI CCWWGG 2 cut(s) 8, 623
FaeI CATG 5 cut(s) 12, 26, 212, 621, 648
FatI CATG 5 cut(s) 8, 22, 208, 617, 644
FauI CCCGC 1 cut(s) 271
FblI GTMKAC 2 cut(s) 128, 177
Fnu4HI GCNGC 5 cut(s) 387, 496, 514, 517, 574
FokI GGATG 1 cut(s) 116
FriOI GRGCYC 2 cut(s) 315, 452
Fsp4HI GCNGC 5 cut(s) 387, 496, 514, 517, 574
FspBI CTAG 4 cut(s) 413, 471, 605, 624
GlaI GCGC 2 cut(s) 345, 577
GluI GCNGC 5 cut(s) 387, 496, 514, 517, 574
GsaI CCCAGC 1 cut(s) 511
GsuI CTGGAG 1 cut(s) 299
HaeII RGCGCY 2 cut(s) 347, 579
HaeIII GGCC 2 cut(s) 108, 450
HapII CCGG 6 cut(s) 110, 219, 347, 454, 504, 543
HgaI GACGC 1 cut(s) 13
HhaI GCGC 2 cut(s) 346, 578
Hin1I GRCGYC 3 cut(s) 5, 300, 344
Hin1II CATG 5 cut(s) 12, 26, 212, 621, 648
Hin6I GCGC 2 cut(s) 344, 576
HinP1I GCGC 2 cut(s) 344, 576
HinfI GANTC 2 cut(s) 174, 229
HpaII CCGG 6 cut(s) 110, 219, 347, 454, 504, 543
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 2 cut(s) 129, 178
Hpy188I TCNGA 2 cut(s) 118, 308
Hpy8I GTNNAC 2 cut(s) 129, 178
HpyCH4III ACNGT 1 cut(s) 530
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 4 cut(s) 46, 162, 246, 644
HpyF10VI GCNNNNNNNGC 6 cut(s) 13, 43, 130, 495, 504, 513
HpyF3I CTNAG 2 cut(s) 273, 653
HpySE526I ACGT 1 cut(s) 300
Hsp92I GRCGYC 3 cut(s) 5, 300, 344
Hsp92II CATG 5 cut(s) 12, 26, 212, 621, 648
HspAI GCGC 2 cut(s) 344, 576
KasI GGCGCC 1 cut(s) 343
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 3 cut(s) 13, 318, 414
Lsp1109I GCAGC 2 cut(s) 525, 585
LweI GCATC 3 cut(s) 25, 214, 351
MaeI CTAG 4 cut(s) 413, 471, 605, 624
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 1 cut(s) 296
MboII GAAGA 4 cut(s) 44, 197, 224, 454
MhlI GDGCHC 4 cut(s) 204, 315, 411, 452
MluCI AATT 2 cut(s) 77, 188
Mly113I GGCGCC 1 cut(s) 344
MlyI GAGTC 2 cut(s) 183, 223
MmeI TCCRAC 1 cut(s) 81
MnlI CCTC 6 cut(s) 187, 314, 343, 346, 364, 563
MslI CAYNNNNRTG 4 cut(s) 21, 167, 207, 264
MspA1I CMGCKG 1 cut(s) 516
MspI CCGG 6 cut(s) 110, 219, 347, 454, 504, 543
MspR9I CCNGG 4 cut(s) 110, 111, 454, 455
MwoI GCNNNNNNNGC 6 cut(s) 13, 43, 130, 495, 504, 513
NarI GGCGCC 1 cut(s) 344
NciI CCSGG 4 cut(s) 110, 111, 454, 455
NcoI CCATGG 1 cut(s) 8
NlaIII CATG 5 cut(s) 12, 26, 212, 621, 648
NlaIV GGNNCC 3 cut(s) 345, 450, 451
NmuCI GTSAC 1 cut(s) 296
NspI RCATGY 1 cut(s) 648
PciSI GCTCTTC 1 cut(s) 27
PkrI GCNGC 5 cut(s) 388, 497, 515, 518, 575
PleI GAGTC 2 cut(s) 182, 223
PluTI GGCGCC 1 cut(s) 347
PpsI GAGTC 2 cut(s) 182, 223
Psp124BI GAGCTC 1 cut(s) 315
PspFI CCCAGC 1 cut(s) 507
PspN4I GGNNCC 3 cut(s) 345, 450, 451
PspOMI GGGCCC 1 cut(s) 448
PspPI GGNCC 3 cut(s) 107, 448, 449
PstI CTGCAG 1 cut(s) 48
PstNI CAGNNNCTG 1 cut(s) 43
RseI CAYNNNNRTG 4 cut(s) 21, 167, 207, 264
SacI GAGCTC 1 cut(s) 315
SapI GCTCTTC 1 cut(s) 27
SatI GCNGC 5 cut(s) 387, 496, 514, 517, 574
Sau96I GGNCC 3 cut(s) 107, 448, 449
SchI GAGTC 2 cut(s) 183, 223
ScrFI CCNGG 4 cut(s) 110, 111, 454, 455
SduI GDGCHC 4 cut(s) 204, 315, 411, 452
SetI ASST 6 cut(s) 31, 296, 303, 315, 334, 375
SfaNI GCATC 3 cut(s) 25, 214, 351
SfcI CTRYAG 1 cut(s) 44
SfoI GGCGCC 1 cut(s) 345
SmaI CCCGGG 2 cut(s) 111, 455
SmiMI CAYNNNNRTG 4 cut(s) 21, 167, 207, 264
Sse9I AATT 2 cut(s) 77, 188
SsiI CCGC 5 cut(s) 205, 278, 386, 495, 516
SspDI GGCGCC 1 cut(s) 343
SspMI CTAG 4 cut(s) 413, 471, 605, 624
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 4 cut(s) 108, 109, 452, 453
StyI CCWWGG 2 cut(s) 8, 623
TaaI ACNGT 1 cut(s) 530
TaiI ACGT 1 cut(s) 303
TaqI TCGA 2 cut(s) 264, 335
TasI AATT 2 cut(s) 77, 188
TauI GCSGC 3 cut(s) 389, 498, 519
TscAI CASTG 1 cut(s) 396
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 2 cut(s) 513, 573
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 2 cut(s) 39, 606
TspGWI ACGGA 1 cut(s) 582
TspMI CCCGGG 2 cut(s) 109, 453
TspRI CASTG 1 cut(s) 396
XapI RAATTY 1 cut(s) 188
XceI RCATGY 1 cut(s) 648
XcmI CCANNNNNNNNNTGG 1 cut(s) 424
XmaI CCCGGG 2 cut(s) 109, 453
XmaJI CCTAGG 1 cut(s) 623
XmiI GTMKAC 2 cut(s) 128, 177
XspI CTAG 4 cut(s) 413, 471, 605, 624
ZraI GACGTC 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.