Rmu_sc0000148.1_g000004

polyribonucleotide nucleotidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000148.1
OG0008286
Physical Location & Seq
Forward (+)
29008 .. 32733
3726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000148.1_g000004.1.cds

Sequence Viewer

Length: 627 bp
atggttcttgattccggcgacgaggcagtagccgtacgagcggccgcgtgtgccttcgtcgaaggccttgacgatgatccccttgcagccgccgtagtgaccttggaggaggatggagaggaggttcagactgagctgaccggtgcgaagacggtaagcgacgacaactgggagatgatggatttgttgctggatcggagcggtctcgatgaagttgaccctcttatcttcattgtccttctccctcactcctctgttctgttcgcgagtcatggtgggtcgagccctgaatttcgaccaccttggacagcccatacgagccgtttgtccctcctcgttcccagaaagaatcgcaaggcatttggtatcagagctttgaccgacgccagagctgctaaggcggttgatgttactcagccttattccgtcaaaattcccgttggtgataggcacattttgattgagacaggtcatattgggagacaagccagtggttctcttaccgtcatagatggagaaactgtcatagtcaagcatttgtcagttgaggaaataggtggcataaaggaggcatttcaattgatggatactgacaacaaaggcaaggtatccctggatcagctttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0004654 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006109 GO:0006139 GO:0006396 GO:0006401 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008299 GO:0008408 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010322 GO:0010323 GO:0010467 GO:0010563 GO:0010565 GO:0010675 GO:0010677 GO:0015994 GO:0015995 GO:0016036 GO:0016070 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016119 GO:0016120 GO:0016122 GO:0016123 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0018130 GO:0019216 GO:0019220 GO:0019222 GO:0019438 GO:0019439 GO:0019747 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031425 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033014 GO:0033554 GO:0034641 GO:0034655 GO:0042214 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045827 GO:0045833 GO:0045912 GO:0045936 GO:0046148 GO:0046246 GO:0046483 GO:0046700 GO:0046890 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051174 GO:0051186 GO:0051188 GO:0051716 GO:0062012 GO:0062014 GO:0065007 GO:0071071 GO:0071072 GO:0071496 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1903725 GO:1903726
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko03018 map00230 map00240 map03018
Pfam Domains
Protein Families
KH_1 PNPase RNase_PH RNase_PH_C S1

Protein Analysis

208

Amino Acids

22.37

Weight (kDa)

4.68

Isoelectric Point (pI)

31.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 41, 201
AccII CGCG 2 cut(s) 47, 266
AciI CCGC 5 cut(s) 41, 45, 90, 201, 401
AclWI GGATC 3 cut(s) 71, 201, 624
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 2 cut(s) 290, 432
AcyI GRCGYC 1 cut(s) 384
AfaI GTAC 1 cut(s) 36
AgeI ACCGGT 1 cut(s) 140
AgsI TTSAA 1 cut(s) 578
AjnI CCWGG 1 cut(s) 612
AloI GAACNNNNNNTCC 2 cut(s) 108, 140
AluBI AGCT 4 cut(s) 136, 374, 392, 622
AluI AGCT 4 cut(s) 136, 374, 392, 622
Alw26I GTCTC 3 cut(s) 209, 458, 475
AlwI GGATC 3 cut(s) 71, 201, 624
AoxI GGCC 2 cut(s) 42, 64
ApeKI GCWGC 2 cut(s) 86, 392
ApoI RAATTY 2 cut(s) 290, 432
AsiGI ACCGGT 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 455
BanII GRGCYC 1 cut(s) 287
BbsI GAAGAC 1 cut(s) 155
BbvI GCAGC 2 cut(s) 98, 379
BccI CCATC 4 cut(s) 107, 172, 506, 577
BceAI ACGGC 3 cut(s) 17, 77, 306
BciT130I CCWGG 1 cut(s) 614
BciVI GTATCC 2 cut(s) 580, 619
BcoDI GTCTC 3 cut(s) 209, 458, 475
BfuI GTATCC 2 cut(s) 580, 619
BisI GCNGC 5 cut(s) 42, 45, 87, 90, 393
BlsI GCNGC 5 cut(s) 43, 46, 88, 91, 394
Bme1390I CCNGG 1 cut(s) 614
BmrFI CCNGG 1 cut(s) 614
BmrI ACTGGG 1 cut(s) 178
BmuI ACTGGG 1 cut(s) 178
BpiI GAAGAC 1 cut(s) 155
Bpu10I CCTNAGC 1 cut(s) 396
BsaHI GRCGYC 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 3 cut(s) 102, 302, 612
BsaWI WCCGGW 1 cut(s) 140
BsaXI ACNNNNNCTCC 4 cut(s) 108, 138, 507, 537
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 2 cut(s) 173, 489
BseBI CCWGG 1 cut(s) 614
BseDI CCNNGG 3 cut(s) 102, 302, 612
BseGI GGATG 1 cut(s) 118
BseMII CTCAG 2 cut(s) 123, 428
BseNI ACTGG 2 cut(s) 173, 489
BseRI GAGGAG 4 cut(s) 122, 134, 241, 323
BseX3I CGGCCG 1 cut(s) 42
BseXI GCAGC 2 cut(s) 98, 379
Bsh1236I CGCG 2 cut(s) 47, 266
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 2 cut(s) 44, 66
BshTI ACCGGT 1 cut(s) 140
BsiEI CGRYCG 1 cut(s) 45
BsiSI CCGG 2 cut(s) 15, 141
BsiWI CGTACG 1 cut(s) 34
BslFI GGGAC 1 cut(s) 313
BsmAI GTCTC 3 cut(s) 209, 458, 475
BsmFI GGGAC 1 cut(s) 313
BsnI GGCC 2 cut(s) 44, 66
Bso31I GGTCTC 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 287
Bsp143I GATC 3 cut(s) 76, 193, 616
Bsp68I TCGCGA 1 cut(s) 266
BspACI CCGC 5 cut(s) 41, 45, 90, 201, 401
BspANI GGCC 2 cut(s) 44, 66
BspCNI CTCAG 2 cut(s) 124, 427
BspFNI CGCG 2 cut(s) 47, 266
BspPI GGATC 3 cut(s) 71, 201, 624
BspTNI GGTCTC 1 cut(s) 209
BsrBI CCGCTC 2 cut(s) 41, 201
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 2 cut(s) 173, 489
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 3 cut(s) 102, 302, 612
BssMI GATC 3 cut(s) 76, 193, 616
BssNI GRCGYC 1 cut(s) 384
BssT1I CCWWGG 2 cut(s) 102, 302
Bst2UI CCWGG 1 cut(s) 614
Bst4CI ACNGT 3 cut(s) 154, 505, 523
BstACI GRCGYC 1 cut(s) 384
BstDEI CTNAG 3 cut(s) 132, 396, 414
BstF5I GGATG 1 cut(s) 118
BstFNI CGCG 2 cut(s) 47, 266
BstKTI GATC 3 cut(s) 79, 196, 619
BstMAI GTCTC 3 cut(s) 209, 458, 475
BstMBI GATC 3 cut(s) 76, 193, 616
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 4 cut(s) 38, 50, 392, 398
BstNI CCWGG 1 cut(s) 614
BstSCI CCNGG 1 cut(s) 612
BstUI CGCG 2 cut(s) 47, 266
BstV1I GCAGC 2 cut(s) 98, 379
BstV2I GAAGAC 1 cut(s) 155
BstZI CGGCCG 1 cut(s) 42
BsuI GTATCC 2 cut(s) 580, 619
BsuRI GGCC 2 cut(s) 44, 66
BtsCI GGATG 1 cut(s) 118
BtsIMutI CAGTG 1 cut(s) 496
BtuMI TCGCGA 1 cut(s) 266
CciNI GCGGCCGC 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 140
CseI GACGC 1 cut(s) 392
Csp6I GTAC 1 cut(s) 35
CspAI ACCGGT 1 cut(s) 140
CviAII CATG 1 cut(s) 272
CviQI GTAC 1 cut(s) 35
DdeI CTNAG 3 cut(s) 132, 396, 414
DpnI GATC 3 cut(s) 78, 195, 618
DpnII GATC 3 cut(s) 76, 193, 616
EaeI YGGCCR 1 cut(s) 42
EagI CGGCCG 1 cut(s) 42
EclXI CGGCCG 1 cut(s) 42
Eco130I CCWWGG 2 cut(s) 102, 302
Eco147I AGGCCT 1 cut(s) 66
Eco24I GRGCYC 1 cut(s) 287
Eco31I GGTCTC 1 cut(s) 209
Eco52I CGGCCG 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 612
EcoT14I CCWWGG 2 cut(s) 102, 302
EcoT38I GRGCYC 1 cut(s) 287
ErhI CCWWGG 2 cut(s) 102, 302
FaeI CATG 1 cut(s) 275
FaiI YATR 6 cut(s) 273, 315, 474, 509, 527, 563
FaqI GGGAC 1 cut(s) 313
FatI CATG 1 cut(s) 271
Fnu4HI GCNGC 5 cut(s) 42, 45, 87, 90, 393
FokI GGATG 1 cut(s) 125
FriOI GRGCYC 1 cut(s) 287
Fsp4HI GCNGC 5 cut(s) 42, 45, 87, 90, 393
GluI GCNGC 5 cut(s) 42, 45, 87, 90, 393
HaeIII GGCC 2 cut(s) 44, 66
HapII CCGG 2 cut(s) 15, 141
HgaI GACGC 1 cut(s) 392
Hin1I GRCGYC 1 cut(s) 384
Hin1II CATG 1 cut(s) 275
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 3 cut(s) 11, 268, 349
HpaII CCGG 2 cut(s) 15, 141
HphI GGTGA 1 cut(s) 455
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 3 cut(s) 129, 198, 371
Hpy188III TCNNGA 3 cut(s) 8, 206, 265
Hpy8I GTNNAC 1 cut(s) 217
Hpy99I CGWCG 4 cut(s) 23, 62, 164, 386
HpyAV CCTTC 3 cut(s) 56, 64, 248
HpyCH4III ACNGT 3 cut(s) 154, 505, 523
HpyCH4V TGCA 1 cut(s) 86
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 50, 392, 398
HpyF3I CTNAG 3 cut(s) 132, 396, 414
Hsp92I GRCGYC 1 cut(s) 384
Hsp92II CATG 1 cut(s) 275
Kzo9I GATC 3 cut(s) 76, 193, 616
LmnI GCTCC 1 cut(s) 198
Lsp1109I GCAGC 2 cut(s) 98, 379
MaeIII GTNAC 2 cut(s) 97, 409
MalI GATC 3 cut(s) 78, 195, 618
MbiI CCGCTC 2 cut(s) 41, 201
MboI GATC 3 cut(s) 76, 193, 616
MboII GAAGA 2 cut(s) 160, 220
MfeI CAATTG 1 cut(s) 578
MhlI GDGCHC 1 cut(s) 287
MluCI AATT 3 cut(s) 290, 432, 578
MlyI GAGTC 1 cut(s) 277
MspI CCGG 2 cut(s) 15, 141
MspR9I CCNGG 1 cut(s) 614
MunI CAATTG 1 cut(s) 578
MvaI CCWGG 1 cut(s) 614
MvnI CGCG 2 cut(s) 47, 266
MwoI GCNNNNNNNGC 4 cut(s) 38, 50, 392, 398
NdeII GATC 3 cut(s) 76, 193, 616
NlaIII CATG 1 cut(s) 275
NmuCI GTSAC 1 cut(s) 97
NotI GCGGCCGC 1 cut(s) 42
NruI TCGCGA 1 cut(s) 266
PceI AGGCCT 1 cut(s) 66
PfeI GAWTC 2 cut(s) 11, 349
Pfl23II CGTACG 1 cut(s) 34
PinAI ACCGGT 1 cut(s) 140
PkrI GCNGC 5 cut(s) 43, 46, 88, 91, 394
PleI GAGTC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 612
PspGI CCWGG 1 cut(s) 612
PspLI CGTACG 1 cut(s) 34
RruI TCGCGA 1 cut(s) 266
RsaI GTAC 1 cut(s) 36
RsaNI GTAC 1 cut(s) 35
SatI GCNGC 5 cut(s) 42, 45, 87, 90, 393
Sau3AI GATC 3 cut(s) 76, 193, 616
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 1 cut(s) 614
SduI GDGCHC 1 cut(s) 287
Sse9I AATT 3 cut(s) 290, 432, 578
SseBI AGGCCT 1 cut(s) 66
SsiI CCGC 5 cut(s) 41, 45, 90, 201, 401
StuI AGGCCT 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 612
StyI CCWWGG 2 cut(s) 102, 302
TaaI ACNGT 3 cut(s) 154, 505, 523
TaqI TCGA 4 cut(s) 60, 207, 281, 295
TaqII GACCGA 1 cut(s) 395
TasI AATT 3 cut(s) 290, 432, 578
TauI GCSGC 3 cut(s) 44, 47, 92
TfiI GAWTC 2 cut(s) 11, 349
TscAI CASTG 1 cut(s) 496
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 2 cut(s) 86, 392
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 2 cut(s) 220, 225
TspGWI ACGGA 1 cut(s) 415
TspRI CASTG 1 cut(s) 496
XapI RAATTY 2 cut(s) 290, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.