Rorug02G0166300

polyribonucleotide nucleotidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008286
Physical Location & Seq
Forward (+)
14898971 .. 14899540
570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0166300.1

Sequence Viewer

Length: 570 bp
ATGGAAAAATTCCTTATAGAGGCTACAATAGGACGTGCACTAGAGGGCTTATTGACAGCAGTTTCACAAGCCAGGGACAGAGCGCATAACTATGAGTCCATTCTTGCACAACTTGAATTCACAATCCGATCCATCGCTCCAAAAATCTATGAAATTGATCGTTTATCTGAAGAACATCTCCCAGATGATGTGGTCCACCGAATACGTCTACAGCTAATTCGAGGTAAGGAGTTGGTCGATAAGTGTTCAAAGTCGGGTTGCTGCGCCTTCTACAACAGCCAGAGGTACTACAAGAAGCTCCAAGAGTTGGATGATTCTCTTGGAAGGCTGATTATGATTGACTTGCAAGTTAAGATGGCATTGGATGTTGTAAGGATTTCGGCTGACATGAAGGAGCTATGTAGGAGATGGGATGATCAAATGCAGAATGTGCATGAAGGGGGAGGACAGAGTGCTTGGATTTTCAAGCTAAGTGGAAGAAAGCTTATTGCTGGACTAAAGAAAATTGTACTGTACAAACCAAACAGAATCCTTCTGAATTTGGTTACTTGTGGAGCTATGGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0004654 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006109 GO:0006139 GO:0006396 GO:0006401 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008299 GO:0008408 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010322 GO:0010323 GO:0010467 GO:0010563 GO:0010565 GO:0010675 GO:0010677 GO:0015994 GO:0015995 GO:0016036 GO:0016070 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016119 GO:0016120 GO:0016122 GO:0016123 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0018130 GO:0019216 GO:0019220 GO:0019222 GO:0019438 GO:0019439 GO:0019747 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031425 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033014 GO:0033554 GO:0034641 GO:0034655 GO:0042214 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045827 GO:0045833 GO:0045912 GO:0045936 GO:0046148 GO:0046246 GO:0046483 GO:0046700 GO:0046890 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051174 GO:0051186 GO:0051188 GO:0051716 GO:0062012 GO:0062014 GO:0065007 GO:0071071 GO:0071072 GO:0071496 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1903725 GO:1903726
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko03018 map00230 map00240 map03018
Pfam Domains
Protein Families
KH_1 PNPase RNase_PH RNase_PH_C S1

Protein Analysis

189

Amino Acids

21.68

Weight (kDa)

8.56

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 134 2.3e-24 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 307
AccI GTMKAC 1 cut(s) 208
AclWI GGATC 1 cut(s) 123
AcsI RAATTY 3 cut(s) 8, 116, 538
AcuI CTGAAG 1 cut(s) 189
AfaI GTAC 3 cut(s) 287, 510, 515
AfiI CCNNNNNNNGG 2 cut(s) 19, 307
AgsI TTSAA 3 cut(s) 116, 249, 466
AjiI CACGTC 1 cut(s) 35
AjnI CCWGG 1 cut(s) 71
AluBI AGCT 6 cut(s) 214, 298, 397, 469, 484, 557
AluI AGCT 6 cut(s) 214, 298, 397, 469, 484, 557
Alw21I GWGCWC 1 cut(s) 40
Alw44I GTGCAC 1 cut(s) 36
AlwI GGATC 1 cut(s) 123
ApaLI GTGCAC 1 cut(s) 36
ApeKI GCWGC 1 cut(s) 261
ApoI RAATTY 3 cut(s) 8, 116, 538
AspLEI GCGC 2 cut(s) 85, 266
AspS9I GGNCC 1 cut(s) 193
AvaII GGWCC 1 cut(s) 193
BaeGI GKGCMC 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 1 cut(s) 248
BccI CCATC 3 cut(s) 140, 349, 402
BciT130I CCWGG 1 cut(s) 73
BclI TGATCA 1 cut(s) 415
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 209
BisI GCNGC 1 cut(s) 262
BlsI GCNGC 1 cut(s) 263
Bme1390I CCNGG 1 cut(s) 73
Bme18I GGWCC 1 cut(s) 193
BmgBI CACGTC 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 193
BmrFI CCNGG 1 cut(s) 73
BsaJI CCNNGG 1 cut(s) 72
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 307
BseBI CCWGG 1 cut(s) 73
BseDI CCNNGG 1 cut(s) 72
BseGI GGATG 3 cut(s) 316, 370, 418
BseLI CCNNNNNNNGG 2 cut(s) 19, 307
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 1 cut(s) 248
BsiHKAI GWGCWC 1 cut(s) 40
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 2 cut(s) 19, 307
BsmFI GGGAC 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 40
Bsp1407I TGTACA 1 cut(s) 513
Bsp143I GATC 3 cut(s) 128, 157, 415
BspPI GGATC 1 cut(s) 123
BsrGI TGTACA 1 cut(s) 513
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 3 cut(s) 128, 157, 415
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 1 cut(s) 513
BstAPI GCANNNNNTGC 1 cut(s) 430
BstAUI TGTACA 1 cut(s) 513
BstDEI CTNAG 1 cut(s) 470
BstENI CCTNNNNNAGG 1 cut(s) 17
BstF5I GGATG 3 cut(s) 316, 370, 418
BstHHI GCGC 2 cut(s) 85, 266
BstKTI GATC 3 cut(s) 131, 160, 418
BstMBI GATC 3 cut(s) 128, 157, 415
BstMWI GCNNNNNNNGC 1 cut(s) 430
BstNI CCWGG 1 cut(s) 73
BstSCI CCNGG 1 cut(s) 71
BstSFI CTRYAG 1 cut(s) 209
BstSLI GKGCMC 1 cut(s) 40
BstV1I GCAGC 1 cut(s) 248
BtgZI GCGATG 1 cut(s) 118
BtrI CACGTC 1 cut(s) 35
BtsCI GGATG 3 cut(s) 316, 370, 418
CfoI GCGC 2 cut(s) 85, 266
Cfr13I GGNCC 1 cut(s) 193
Csp6I GTAC 3 cut(s) 286, 509, 514
CspCI CAANNNNNGTGG 2 cut(s) 454, 489
CviAII CATG 2 cut(s) 388, 434
CviQI GTAC 3 cut(s) 286, 509, 514
DdeI CTNAG 1 cut(s) 470
DpnI GATC 3 cut(s) 130, 159, 417
DpnII GATC 3 cut(s) 128, 157, 415
Eco47I GGWCC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 189
EcoNI CCTNNNNNAGG 1 cut(s) 17
EcoRI GAATTC 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 71
FaeI CATG 2 cut(s) 391, 437
FaiI YATR 9 cut(s) 17, 87, 93, 150, 335, 389, 400, 435, 560
FaqI GGGAC 1 cut(s) 89
FatI CATG 2 cut(s) 387, 433
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 1 cut(s) 208
Fnu4HI GCNGC 1 cut(s) 262
FokI GGATG 3 cut(s) 323, 377, 425
Fsp4HI GCNGC 1 cut(s) 262
FspBI CTAG 1 cut(s) 41
GlaI GCGC 2 cut(s) 84, 265
GluI GCNGC 1 cut(s) 262
HhaI GCGC 2 cut(s) 85, 266
Hin1II CATG 2 cut(s) 391, 437
Hin6I GCGC 2 cut(s) 83, 264
HinP1I GCGC 2 cut(s) 83, 264
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 3 cut(s) 95, 314, 528
Hpy166II GTNNAC 3 cut(s) 38, 196, 209
Hpy188I TCNGA 3 cut(s) 128, 169, 537
Hpy8I GTNNAC 3 cut(s) 38, 196, 209
HpyAV CCTTC 5 cut(s) 277, 318, 385, 431, 542
HpyCH4III ACNGT 1 cut(s) 513
HpyCH4IV ACGT 2 cut(s) 34, 205
HpyCH4V TGCA 5 cut(s) 38, 107, 346, 424, 433
HpyF10VI GCNNNNNNNGC 1 cut(s) 430
HpyF3I CTNAG 1 cut(s) 470
HpySE526I ACGT 2 cut(s) 34, 205
Hsp92II CATG 2 cut(s) 391, 437
HspAI GCGC 2 cut(s) 83, 264
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 3 cut(s) 128, 157, 415
LmnI GCTCC 4 cut(s) 142, 303, 394, 554
LpnPI CCDG 5 cut(s) 58, 85, 195, 293, 477
Lsp1109I GCAGC 1 cut(s) 248
MaeI CTAG 1 cut(s) 41
MaeII ACGT 2 cut(s) 34, 205
MaeIII GTNAC 1 cut(s) 544
MalI GATC 3 cut(s) 130, 159, 417
MboI GATC 3 cut(s) 128, 157, 415
MboII GAAGA 2 cut(s) 182, 489
MhlI GDGCHC 1 cut(s) 40
MluCI AATT 6 cut(s) 8, 116, 153, 216, 504, 538
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 5 cut(s) 13, 37, 215, 276, 437
MseI TTAA 2 cut(s) 351, 568
MslI CAYNNNNRTG 1 cut(s) 90
MspR9I CCNGG 1 cut(s) 73
MvaI CCWGG 1 cut(s) 73
MwoI GCNNNNNNNGC 1 cut(s) 430
NdeII GATC 3 cut(s) 128, 157, 415
NlaIII CATG 2 cut(s) 391, 437
PfeI GAWTC 2 cut(s) 314, 528
PflMI CCANNNNNTGG 1 cut(s) 307
PkrI GCNGC 1 cut(s) 263
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Psp6I CCWGG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 71
PspPI GGNCC 1 cut(s) 193
RsaI GTAC 3 cut(s) 287, 510, 515
RsaNI GTAC 3 cut(s) 286, 509, 514
RseI CAYNNNNRTG 1 cut(s) 90
SaqAI TTAA 2 cut(s) 351, 568
SatI GCNGC 1 cut(s) 262
Sau3AI GATC 3 cut(s) 128, 157, 415
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 1 cut(s) 73
SduI GDGCHC 1 cut(s) 40
SfcI CTRYAG 1 cut(s) 209
SinI GGWCC 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 90
Sse9I AATT 6 cut(s) 8, 116, 153, 216, 504, 538
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 1 cut(s) 71
TaaI ACNGT 1 cut(s) 513
TaiI ACGT 2 cut(s) 37, 208
TaqI TCGA 2 cut(s) 220, 237
TasI AATT 6 cut(s) 8, 116, 153, 216, 504, 538
TatI WGTACW 2 cut(s) 508, 513
TfiI GAWTC 2 cut(s) 314, 528
Tru1I TTAA 2 cut(s) 351, 568
Tru9I TTAA 2 cut(s) 351, 568
TseI GCWGC 1 cut(s) 261
TspDTI ATGAA 3 cut(s) 165, 404, 450
Van91I CCANNNNNTGG 1 cut(s) 307
VneI GTGCAC 1 cut(s) 36
VpaK11BI GGWCC 1 cut(s) 193
XagI CCTNNNNNAGG 1 cut(s) 17
XapI RAATTY 3 cut(s) 8, 116, 538
XmiI GTMKAC 1 cut(s) 208
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.