Rroxscaffold_4G00308320

polyribonucleotide nucleotidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0008286
Physical Location & Seq
Reverse (-)
29847775 .. 29854186
6412 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00308320.1

Sequence Viewer

Length: 831 bp
ATGCTACTACACTTCTCGGACCCTCTTATCTTCATCGTCCTTCTCCCTCACTCGTTCGTTCGCGAGTCATGGTGGGTCGAGCCTCGAATTTCGACCACCTTGGACAGCCCATACGAGCCGGTGGCCGTGTCACTCGACCTCAATTTGCTGGACAGAGATCCCGCAGTTAGCCGCCATGTTATGGGCTTCGAGCCGAGCAAATTACGATTCCGTTTCGTTATCCCGGATTATAGGTTTGTTTTTGGTAAGCAAGCTTGGAGATTTGATTTTGTGGGTACACTTTGTTTTTTGCAGTTTGTCCTTCCTCGTTCCCGAAGGAATCACAAGGCATTTGGCATCGTAGCTTTGACCGACACCGGAACTGCTGCGGCTGTTGATGTTACTCAGCCTTATTCCGTCAAAATCCCCGTTGGTGATAGGCACATTTTGATTGAGACAGGTCATATTGGGAGACAAGCCGGTGGTTCTCTTACCGTCATAGATGGAGAAACTGCCAAGGTGACAAGTAACGATGTAGTTAGAGCTCTAACATGTTGTGGGAAAGACCACGGTGGAGATACCGCATGGGTGGGAAAGACCACATGCAGTGAGGAGGATTGCACGGGTGGGAAAGACCACATGCAAATGATTGCTTTTGCTATAAGTTATTATGTGATTGCATTACCACAAAAGAAGGCCCCAAATGTTCCACTTGGTGAGACTGTCATAGTCGAGCATTTGTCAGTTGAGGAAATAGCTGGCATAAAGGAGGCATTTCAATTGATGGATACTGACAACAAAGGCAACGTATCCCTGGATCAGCTTCGAAATGGAATACAAGAACTCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0004654 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006109 GO:0006139 GO:0006396 GO:0006401 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008299 GO:0008408 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010322 GO:0010323 GO:0010467 GO:0010563 GO:0010565 GO:0010675 GO:0010677 GO:0015994 GO:0015995 GO:0016036 GO:0016070 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016119 GO:0016120 GO:0016122 GO:0016123 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0018130 GO:0019216 GO:0019220 GO:0019222 GO:0019438 GO:0019439 GO:0019747 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031425 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033014 GO:0033554 GO:0034641 GO:0034655 GO:0042214 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045827 GO:0045833 GO:0045912 GO:0045936 GO:0046148 GO:0046246 GO:0046483 GO:0046700 GO:0046890 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051174 GO:0051186 GO:0051188 GO:0051716 GO:0062012 GO:0062014 GO:0065007 GO:0071071 GO:0071072 GO:0071496 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1903725 GO:1903726
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko03018 map00230 map00240 map03018
Pfam Domains
Protein Families
KH_1 PNPase RNase_PH RNase_PH_C S1

Protein Analysis

276

Amino Acids

30.65

Weight (kDa)

5.98

Isoelectric Point (pI)

34.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 181
AccII CGCG 1 cut(s) 63
AciI CCGC 4 cut(s) 162, 172, 368, 561
AclWI GGATC 2 cut(s) 152, 804
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 1 cut(s) 87
AdeI CACNNNGTG 1 cut(s) 695
AfaI GTAC 1 cut(s) 277
AfiI CCNNNNNNNGG 1 cut(s) 181
AflIII ACRYGT 1 cut(s) 530
AgsI TTSAA 1 cut(s) 758
AjnI CCWGG 1 cut(s) 792
AluBI AGCT 5 cut(s) 254, 344, 524, 737, 802
AluI AGCT 5 cut(s) 254, 344, 524, 737, 802
Alw21I GWGCWC 1 cut(s) 526
Alw26I GTCTC 3 cut(s) 428, 445, 692
AlwI GGATC 2 cut(s) 152, 804
AoxI GGCC 2 cut(s) 123, 675
ApeKI GCWGC 1 cut(s) 365
ApoI RAATTY 1 cut(s) 87
AspS9I GGNCC 2 cut(s) 19, 676
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 3 cut(s) 425, 511, 707
AsuII TTCGAA 1 cut(s) 805
AvaII GGWCC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 526
Bbv12I GWGCWC 1 cut(s) 526
BbvI GCAGC 1 cut(s) 352
BccI CCATC 2 cut(s) 476, 757
BceAI ACGGC 1 cut(s) 110
BciT130I CCWGG 1 cut(s) 794
BciVI GTATCC 2 cut(s) 760, 799
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 3 cut(s) 428, 445, 692
BfuI GTATCC 2 cut(s) 760, 799
BisI GCNGC 3 cut(s) 172, 366, 369
BlsI GCNGC 3 cut(s) 173, 367, 370
Bme1390I CCNGG 2 cut(s) 224, 794
Bme18I GGWCC 1 cut(s) 19
BmgT120I GGNCC 2 cut(s) 19, 676
BmiI GGNNCC 2 cut(s) 21, 678
BmrFI CCNGG 2 cut(s) 224, 794
BmsI GCATC 1 cut(s) 345
Bpu14I TTCGAA 1 cut(s) 805
BpuMI CCSGG 1 cut(s) 224
BsaJI CCNNGG 4 cut(s) 99, 495, 547, 792
BsaWI WCCGGW 1 cut(s) 356
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse118I RCCGGY 2 cut(s) 118, 458
BseBI CCWGG 1 cut(s) 794
BseDI CCNNGG 4 cut(s) 99, 495, 547, 792
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMII CTCAG 1 cut(s) 398
BseRI GAGGAG 1 cut(s) 605
BseXI GCAGC 1 cut(s) 352
Bsh1236I CGCG 1 cut(s) 63
BshFI GGCC 2 cut(s) 125, 677
BsiHKAI GWGCWC 1 cut(s) 526
BsiSI CCGG 4 cut(s) 119, 224, 357, 459
BslI CCNNNNNNNGG 1 cut(s) 181
BsmAI GTCTC 3 cut(s) 428, 445, 692
BsnI GGCC 2 cut(s) 125, 677
Bsp119I TTCGAA 1 cut(s) 805
Bsp1286I GDGCHC 1 cut(s) 526
Bsp143I GATC 2 cut(s) 157, 796
Bsp68I TCGCGA 1 cut(s) 63
BspACI CCGC 4 cut(s) 162, 172, 368, 561
BspANI GGCC 2 cut(s) 125, 677
BspCNI CTCAG 1 cut(s) 397
BspFNI CGCG 1 cut(s) 63
BspLI GGNNCC 2 cut(s) 21, 678
BspPI GGATC 2 cut(s) 152, 804
BspT104I TTCGAA 1 cut(s) 805
BsrFI RCCGGY 2 cut(s) 118, 458
BssAI RCCGGY 2 cut(s) 118, 458
BssECI CCNNGG 4 cut(s) 99, 495, 547, 792
BssMI GATC 2 cut(s) 157, 796
BssT1I CCWWGG 2 cut(s) 99, 495
Bst2UI CCWGG 1 cut(s) 794
Bst4CI ACNGT 3 cut(s) 475, 551, 703
BstBI TTCGAA 1 cut(s) 805
BstC8I GCNNGC 2 cut(s) 252, 739
BstDEI CTNAG 2 cut(s) 384, 824
BstDSI CCRYGG 1 cut(s) 547
BstFNI CGCG 1 cut(s) 63
BstKTI GATC 2 cut(s) 160, 799
BstMAI GTCTC 3 cut(s) 428, 445, 692
BstMBI GATC 2 cut(s) 157, 796
BstNI CCWGG 1 cut(s) 794
BstNSI RCATGY 3 cut(s) 534, 585, 622
BstSCI CCNGG 2 cut(s) 222, 792
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 1 cut(s) 352
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
BsuI GTATCC 2 cut(s) 760, 799
BsuRI GGCC 2 cut(s) 125, 677
BtgI CCRYGG 1 cut(s) 547
BtsI GCAGTG 1 cut(s) 592
BtsIMutI CAGTG 1 cut(s) 592
BtuMI TCGCGA 1 cut(s) 63
Cac8I GCNNGC 2 cut(s) 252, 739
Cfr10I RCCGGY 2 cut(s) 118, 458
Cfr13I GGNCC 2 cut(s) 19, 676
Csp6I GTAC 1 cut(s) 276
CviAII CATG 6 cut(s) 69, 176, 531, 564, 582, 619
CviQI GTAC 1 cut(s) 276
DdeI CTNAG 2 cut(s) 384, 824
DpnI GATC 2 cut(s) 159, 798
DpnII GATC 2 cut(s) 157, 796
DraIII CACNNNGTG 1 cut(s) 695
EaeI YGGCCR 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 524
Eco130I CCWWGG 2 cut(s) 99, 495
Eco24I GRGCYC 1 cut(s) 526
Eco47I GGWCC 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 524
EcoICRI GAGCTC 1 cut(s) 524
EcoO109I RGGNCCY 1 cut(s) 676
EcoRII CCWGG 1 cut(s) 792
EcoT14I CCWWGG 2 cut(s) 99, 495
EcoT38I GRGCYC 1 cut(s) 526
ErhI CCWWGG 2 cut(s) 99, 495
FaeI CATG 6 cut(s) 72, 179, 534, 567, 585, 622
FatI CATG 6 cut(s) 68, 175, 530, 563, 581, 618
FauI CCCGC 1 cut(s) 169
Fnu4HI GCNGC 3 cut(s) 172, 366, 369
FriOI GRGCYC 1 cut(s) 526
Fsp4HI GCNGC 3 cut(s) 172, 366, 369
GluI GCNGC 3 cut(s) 172, 366, 369
HaeIII GGCC 2 cut(s) 125, 677
HapII CCGG 4 cut(s) 119, 224, 357, 459
Hin1II CATG 6 cut(s) 72, 179, 534, 567, 585, 622
HindIII AAGCTT 1 cut(s) 252
HinfI GANTC 3 cut(s) 65, 207, 319
HpaII CCGG 4 cut(s) 119, 224, 357, 459
HphI GGTGA 3 cut(s) 425, 511, 707
Hpy166II GTNNAC 1 cut(s) 278
Hpy188I TCNGA 2 cut(s) 19, 827
Hpy188III TCNNGA 2 cut(s) 62, 312
Hpy8I GTNNAC 1 cut(s) 278
HpyAV CCTTC 4 cut(s) 50, 309, 311, 667
HpyCH4III ACNGT 3 cut(s) 475, 551, 703
HpyCH4IV ACGT 1 cut(s) 786
HpyCH4V TGCA 5 cut(s) 292, 585, 600, 622, 659
HpyF3I CTNAG 2 cut(s) 384, 824
HpySE526I ACGT 1 cut(s) 786
Hsp92II CATG 6 cut(s) 72, 179, 534, 567, 585, 622
Kzo9I GATC 2 cut(s) 157, 796
LpnPI CCDG 9 cut(s) 132, 134, 237, 370, 423, 472, 723, 779, 806
Lsp1109I GCAGC 1 cut(s) 352
LweI GCATC 1 cut(s) 345
MaeII ACGT 1 cut(s) 786
MaeIII GTNAC 4 cut(s) 129, 379, 499, 506
MalI GATC 2 cut(s) 159, 798
MboI GATC 2 cut(s) 157, 796
MboII GAAGA 1 cut(s) 22
MfeI CAATTG 1 cut(s) 758
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 1 cut(s) 526
MluCI AATT 4 cut(s) 87, 142, 200, 758
MlyI GAGTC 1 cut(s) 74
MnlI CCTC 9 cut(s) 33, 57, 93, 149, 315, 583, 586, 721, 742
MslI CAYNNNNRTG 1 cut(s) 623
MspI CCGG 4 cut(s) 119, 224, 357, 459
MspR9I CCNGG 2 cut(s) 224, 794
MunI CAATTG 1 cut(s) 758
MvaI CCWGG 1 cut(s) 794
MvnI CGCG 1 cut(s) 63
NciI CCSGG 1 cut(s) 224
NdeII GATC 2 cut(s) 157, 796
NlaIII CATG 6 cut(s) 72, 179, 534, 567, 585, 622
NlaIV GGNNCC 2 cut(s) 21, 678
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 2 cut(s) 129, 499
NruI TCGCGA 1 cut(s) 63
NspI RCATGY 3 cut(s) 534, 585, 622
NspV TTCGAA 1 cut(s) 805
PciI ACATGT 1 cut(s) 530
PfeI GAWTC 2 cut(s) 207, 319
PflMI CCANNNNNTGG 1 cut(s) 181
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 3 cut(s) 173, 367, 370
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
PscI ACATGT 1 cut(s) 530
Psp124BI GAGCTC 1 cut(s) 526
Psp6I CCWGG 1 cut(s) 792
PspGI CCWGG 1 cut(s) 792
PspN4I GGNNCC 2 cut(s) 21, 678
PspPI GGNCC 2 cut(s) 19, 676
PsuI RGATCY 1 cut(s) 157
RruI TCGCGA 1 cut(s) 63
RsaI GTAC 1 cut(s) 277
RsaNI GTAC 1 cut(s) 276
RseI CAYNNNNRTG 1 cut(s) 623
SacI GAGCTC 1 cut(s) 526
SatI GCNGC 3 cut(s) 172, 366, 369
Sau3AI GATC 2 cut(s) 157, 796
Sau96I GGNCC 2 cut(s) 19, 676
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 2 cut(s) 224, 794
SduI GDGCHC 1 cut(s) 526
SfaNI GCATC 1 cut(s) 345
SfuI TTCGAA 1 cut(s) 805
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 623
Sse9I AATT 4 cut(s) 87, 142, 200, 758
SsiI CCGC 4 cut(s) 162, 172, 368, 561
SstI GAGCTC 1 cut(s) 526
StyD4I CCNGG 2 cut(s) 222, 792
StyI CCWWGG 2 cut(s) 99, 495
TaaI ACNGT 3 cut(s) 475, 551, 703
TaiI ACGT 1 cut(s) 789
TaqI TCGA 7 cut(s) 78, 85, 92, 135, 189, 711, 805
TaqII GACCGA 1 cut(s) 365
TasI AATT 4 cut(s) 87, 142, 200, 758
TauI GCSGC 2 cut(s) 174, 371
TfiI GAWTC 2 cut(s) 207, 319
TscAI CASTG 1 cut(s) 592
TseFI GTSAC 2 cut(s) 129, 499
TseI GCWGC 1 cut(s) 365
Tsp45I GTSAC 2 cut(s) 129, 499
TspDTI ATGAA 1 cut(s) 22
TspGWI ACGGA 2 cut(s) 200, 385
TspRI CASTG 1 cut(s) 592
Van91I CCANNNNNTGG 1 cut(s) 181
VpaK11BI GGWCC 1 cut(s) 19
XapI RAATTY 1 cut(s) 87
XceI RCATGY 3 cut(s) 534, 585, 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.