Rmu_sc0000243.1_g000005

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000243.1
OG0013533
Physical Location & Seq
Reverse (-)
19417 .. 23266
3850 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1185 bp
atggattcccgggacgccccgccgcagcagcctccgccgcagcaacccaacatgatggtgggccccaattcctacccttcccccatggccaaccccaaccccaacaacaacgccaccgccaccgccaacagcaacaacaacaacaactcctcggccgcgatgttgggcggcggcgggccgaattcgggtcggttccagttcaacacggtggcccagcagcagcccgcatcgaagccgcagccgctcgattccatgagcccgagcccgttcgacggatcgttgaggccgtgcggcggcggcggcggcggcagcggagggtttagcatcgactcatctacggcctcggcggggaagaagaagcgaggcaggcccagaaagtactcccctgatggcaacattgcattgggattggcgccaactcagattcctgcctcggctcccgttgcggccgccgccggcccccacggagaatccagcggcaccatgtcgtcggatccccccgccaagaagaacagggggcgcccgccgggctccggaaagaagcagctcgacgctttgggagctggtggagttgggttcacgcctcatgtcattatggtgcaagccggcgaggatatagcatcaaaggttatgtccttttcacagcaaggaccacgcacagtttgtatcctttctgcaaatggtccaatcggcaatgttacccttcgccagccatcaatgtctggtggtacagtcacatatgagggtcgatttgagattatttctctgtcaggctcctacttgttctctgaaaataatggtaaccgcagcagaactggtggtctgagtgtgtccctggcagggtctgatggttcagtgctgggcggtggagttgcgggcatgctattggcagcaggcccggtacaggtgattgtgggtagctttattgcggaagggaaaaaatctagctccaatttgctaaaatcagggccttcttctccaccaccatctcaaatgttgaactttggtgcacctatgacagcagctagcccttcctctcaaggaggaggctcaactgagtcgtctgatgagaatggtagtagccctcttaacaaggccccacctgtgctttacagtaaccctagtcaacccatgcataatatcacgatgtaccaacaattatgggctgggcaaactcaacagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

394

Amino Acids

39.7

Weight (kDa)

9.57

Isoelectric Point (pI)

63.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 194 - 309 3.5e-24 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013533)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17950 AT5G46640
fragaria_vesca FvH4_5g22000
malus_domestica MD06G1033700.v1.1 MD16G1274800.v1.1
prunus_persica Prupe.5G037000_v2.0.a1
pyrus_communis pycom06g02780 pycom16g24490
rosa_chinensis RchiOBHm_Chr7g0209501
rosa_laevigata RLG00000003123
rosa_multiflora Rmu_sc0000243.1_g000005
rosa_roxburghii Rroxscaffold_3G00249310
rosa_rugosa Rorug07G0114000
rosa_samantha Rh7AG248000 Rh7BG242500 Rh7CG263500 Rh7DG254100
rosa_wichuraiana Rw7G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 412, 479, 519
AccBSI CCGCTC 1 cut(s) 244
AccII CGCG 1 cut(s) 158
AccIII TCCGGA 1 cut(s) 533
AclWI GGATC 3 cut(s) 283, 488, 501
AcoI YGGCCR 3 cut(s) 87, 153, 447
AcsI RAATTY 1 cut(s) 181
AcyI GRCGYC 3 cut(s) 15, 413, 520
AfaI GTAC 4 cut(s) 380, 730, 903, 1151
AfiI CCNNNNNNNGG 7 cut(s) 185, 272, 293, 348, 533, 840, 904
AgsI TTSAA 2 cut(s) 202, 1000
AjnI CCWGG 1 cut(s) 834
AluBI AGCT 5 cut(s) 547, 563, 921, 948, 1025
AluI AGCT 5 cut(s) 547, 563, 921, 948, 1025
Alw21I GWGCWC 1 cut(s) 1012
Alw44I GTGCAC 1 cut(s) 1008
AlwI GGATC 3 cut(s) 283, 488, 501
AlwNI CAGNNNCTG 1 cut(s) 845
Ama87I CYCGRG 2 cut(s) 9, 259
Aor13HI TCCGGA 1 cut(s) 533
ApaI GGGCCC 1 cut(s) 65
ApaLI GTGCAC 1 cut(s) 1008
ApoI RAATTY 1 cut(s) 181
ArsI GACNNNNNNTTYG 2 cut(s) 617, 649
AspLEI GCGC 2 cut(s) 415, 522
AsuC2I CCSGG 4 cut(s) 10, 11, 528, 899
AsuHPI GGTGA 1 cut(s) 919
AsuNHI GCTAGC 1 cut(s) 1025
AvaI CYCGRG 2 cut(s) 9, 259
AvaII GGWCC 2 cut(s) 650, 683
BaeGI GKGCMC 2 cut(s) 65, 1012
BalI TGGCCA 1 cut(s) 89
BamHI GGATCC 1 cut(s) 493
BanI GGYRCC 3 cut(s) 412, 479, 519
BanII GRGCYC 4 cut(s) 65, 260, 266, 533
Bbv12I GWGCWC 1 cut(s) 1012
BccI CCATC 5 cut(s) 49, 383, 721, 842, 994
BceAI ACGGC 2 cut(s) 271, 354
BciT130I CCWGG 1 cut(s) 836
BciVI GTATCC 1 cut(s) 677
BcnI CCSGG 4 cut(s) 10, 11, 528, 899
BfaI CTAG 3 cut(s) 945, 1026, 1122
BfoI RGCGCY 2 cut(s) 416, 523
BfuI GTATCC 1 cut(s) 677
BglI GCCNNNNNGGC 1 cut(s) 528
BmcAI AGTACT 1 cut(s) 380
Bme1390I CCNGG 5 cut(s) 10, 11, 528, 836, 899
Bme18I GGWCC 2 cut(s) 650, 683
BmeT110I CYCGRG 2 cut(s) 9, 259
BmrFI CCNGG 5 cut(s) 10, 11, 528, 836, 899
BmsI GCATC 3 cut(s) 236, 333, 629
BmtI GCTAGC 1 cut(s) 1029
BpuEI CTTGAG 1 cut(s) 1023
BpuMI CCSGG 4 cut(s) 10, 11, 528, 899
BsaHI GRCGYC 3 cut(s) 15, 413, 520
BsaJI CCNNGG 7 cut(s) 9, 84, 150, 342, 432, 463, 834
BsaWI WCCGGW 1 cut(s) 533
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 7 cut(s) 185, 272, 293, 348, 533, 840, 904
Bse118I RCCGGY 2 cut(s) 455, 605
Bse1I ACTGG 2 cut(s) 196, 820
Bse3DI GCAATG 2 cut(s) 396, 700
BseAI TCCGGA 1 cut(s) 533
BseBI CCWGG 1 cut(s) 836
BseDI CCNNGG 7 cut(s) 9, 84, 150, 342, 432, 463, 834
BseLI CCNNNNNNNGG 7 cut(s) 185, 272, 293, 348, 533, 840, 904
BseMI GCAATG 2 cut(s) 396, 700
BseMII CTCAG 3 cut(s) 434, 815, 1047
BseNI ACTGG 2 cut(s) 196, 820
BseRI GAGGAG 2 cut(s) 139, 1059
BseSI GKGCMC 2 cut(s) 65, 1012
BseX3I CGGCCG 2 cut(s) 153, 447
BseYI CCCAGC 3 cut(s) 213, 859, 1166
Bsh1236I CGCG 1 cut(s) 158
Bsh1285I CGRYCG 2 cut(s) 156, 450
BshNI GGYRCC 3 cut(s) 412, 479, 519
BsiEI CGRYCG 2 cut(s) 156, 450
BsiHKAI GWGCWC 1 cut(s) 1012
BsiHKCI CYCGRG 2 cut(s) 9, 259
BsiSI CCGG 6 cut(s) 10, 456, 527, 534, 606, 899
BslFI GGGAC 2 cut(s) 26, 817
BslI CCNNNNNNNGG 7 cut(s) 185, 272, 293, 348, 533, 840, 904
BsmFI GGGAC 2 cut(s) 26, 817
BsoBI CYCGRG 2 cut(s) 9, 259
Bsp120I GGGCCC 1 cut(s) 61
Bsp1286I GDGCHC 5 cut(s) 65, 260, 266, 533, 1012
Bsp13I TCCGGA 1 cut(s) 533
Bsp143I GATC 2 cut(s) 275, 493
Bsp19I CCATGG 1 cut(s) 84
BspCNI CTCAG 3 cut(s) 433, 816, 1048
BspEI TCCGGA 1 cut(s) 533
BspFNI CGCG 1 cut(s) 158
BspOI GCTAGC 1 cut(s) 1029
BspPI GGATC 3 cut(s) 283, 488, 501
BspT107I GGYRCC 3 cut(s) 412, 479, 519
BsrBI CCGCTC 1 cut(s) 244
BsrDI GCAATG 2 cut(s) 396, 700
BsrFI RCCGGY 2 cut(s) 455, 605
BsrI ACTGG 2 cut(s) 196, 820
BssAI RCCGGY 2 cut(s) 455, 605
BssECI CCNNGG 7 cut(s) 9, 84, 150, 342, 432, 463, 834
BssMI GATC 2 cut(s) 275, 493
BssNI GRCGYC 3 cut(s) 15, 413, 520
BssT1I CCWWGG 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 836
Bst4CI ACNGT 5 cut(s) 208, 661, 733, 1115, 1182
BstACI GRCGYC 3 cut(s) 15, 413, 520
BstDEI CTNAG 3 cut(s) 420, 824, 1056
BstDSI CCRYGG 2 cut(s) 84, 463
BstEII GGTNACC 1 cut(s) 800
BstFNI CGCG 1 cut(s) 158
BstH2I RGCGCY 2 cut(s) 416, 523
BstHHI GCGC 2 cut(s) 415, 522
BstKTI GATC 2 cut(s) 278, 496
BstMBI GATC 2 cut(s) 275, 493
BstMCI CGRYCG 2 cut(s) 156, 450
BstNI CCWGG 1 cut(s) 836
BstNSI RCATGY 1 cut(s) 883
BstPI GGTNACC 1 cut(s) 800
BstSCI CCNGG 5 cut(s) 8, 9, 526, 834, 897
BstSLI GKGCMC 2 cut(s) 65, 1012
BstUI CGCG 1 cut(s) 158
BstX2I RGATCY 1 cut(s) 493
BstXI CCANNNNNNTGG 1 cut(s) 55
BstYI RGATCY 1 cut(s) 493
BstZI CGGCCG 2 cut(s) 153, 447
BsuI GTATCC 1 cut(s) 677
BtgI CCRYGG 2 cut(s) 84, 463
BtgZI GCGATG 1 cut(s) 173
BtsIMutI CAGTG 1 cut(s) 861
CaiI CAGNNNCTG 1 cut(s) 845
CciNI GCGGCCGC 1 cut(s) 447
CfoI GCGC 2 cut(s) 415, 522
Cfr10I RCCGGY 2 cut(s) 455, 605
Cfr9I CCCGGG 1 cut(s) 9
CseI GACGC 2 cut(s) 23, 560
Csp6I GTAC 4 cut(s) 379, 729, 902, 1150
CviAII CATG 7 cut(s) 52, 85, 253, 484, 587, 880, 1132
CviQI GTAC 4 cut(s) 379, 729, 902, 1150
DdeI CTNAG 3 cut(s) 420, 824, 1056
DinI GGCGCC 2 cut(s) 414, 521
DpnI GATC 2 cut(s) 277, 495
DpnII GATC 2 cut(s) 275, 493
EaeI YGGCCR 3 cut(s) 87, 153, 447
EagI CGGCCG 2 cut(s) 153, 447
EciI GGCGGA 1 cut(s) 24
EclXI CGGCCG 2 cut(s) 153, 447
Eco130I CCWWGG 1 cut(s) 84
Eco24I GRGCYC 4 cut(s) 65, 260, 266, 533
Eco47I GGWCC 2 cut(s) 650, 683
Eco52I CGGCCG 2 cut(s) 153, 447
Eco88I CYCGRG 2 cut(s) 9, 259
Eco91I GGTNACC 1 cut(s) 800
EcoO109I RGGNCCY 3 cut(s) 62, 968, 1096
EcoO65I GGTNACC 1 cut(s) 800
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 834
EcoT14I CCWWGG 1 cut(s) 84
EcoT22I ATGCAT 1 cut(s) 1137
EcoT38I GRGCYC 4 cut(s) 65, 260, 266, 533
EgeI GGCGCC 2 cut(s) 414, 521
EheI GGCGCC 2 cut(s) 414, 521
ErhI CCWWGG 1 cut(s) 84
FaeI CATG 7 cut(s) 55, 88, 256, 487, 590, 883, 1135
FaqI GGGAC 2 cut(s) 26, 817
FatI CATG 7 cut(s) 51, 84, 252, 483, 586, 879, 1131
FauI CCCGC 7 cut(s) 27, 167, 232, 340, 508, 531, 868
FauNDI CATATG 1 cut(s) 739
FriOI GRGCYC 4 cut(s) 65, 260, 266, 533
FspBI CTAG 3 cut(s) 945, 1026, 1122
GlaI GCGC 2 cut(s) 414, 521
GsaI CCCAGC 3 cut(s) 217, 863, 1170
HaeII RGCGCY 2 cut(s) 416, 523
HapII CCGG 6 cut(s) 10, 456, 527, 534, 606, 899
HgaI GACGC 2 cut(s) 23, 560
HhaI GCGC 2 cut(s) 415, 522
Hin1I GRCGYC 3 cut(s) 15, 413, 520
Hin1II CATG 7 cut(s) 55, 88, 256, 487, 590, 883, 1135
Hin6I GCGC 2 cut(s) 413, 520
HinP1I GCGC 2 cut(s) 413, 520
HincII GTYRAC 1 cut(s) 1127
HindII GTYRAC 1 cut(s) 1127
HinfI GANTC 6 cut(s) 5, 248, 329, 424, 470, 1058
HpaII CCGG 6 cut(s) 10, 456, 527, 534, 606, 899
HphI GGTGA 1 cut(s) 919
Hpy166II GTNNAC 3 cut(s) 579, 1010, 1127
Hpy188I TCNGA 6 cut(s) 423, 493, 790, 825, 847, 1066
Hpy188III TCNNGA 2 cut(s) 534, 1144
Hpy8I GTNNAC 3 cut(s) 579, 1010, 1127
Hpy99I CGWCG 3 cut(s) 275, 493, 554
HpyAV CCTTC 5 cut(s) 87, 713, 926, 981, 1041
HpyCH4III ACNGT 5 cut(s) 208, 661, 733, 1115, 1182
HpyCH4V TGCA 5 cut(s) 401, 601, 677, 1010, 1135
HpyF3I CTNAG 3 cut(s) 420, 824, 1056
Hsp92I GRCGYC 3 cut(s) 15, 413, 520
Hsp92II CATG 7 cut(s) 55, 88, 256, 487, 590, 883, 1135
HspAI GCGC 2 cut(s) 413, 520
KasI GGCGCC 2 cut(s) 412, 519
Kpn2I TCCGGA 1 cut(s) 533
KroI GCCGGC 2 cut(s) 455, 605
KroNI GCCGGC 2 cut(s) 457, 607
Kzo9I GATC 2 cut(s) 275, 493
LmnI GCTCC 5 cut(s) 442, 536, 560, 779, 953
LweI GCATC 3 cut(s) 236, 333, 629
MaeI CTAG 3 cut(s) 945, 1026, 1122
MaeIII GTNAC 4 cut(s) 697, 733, 800, 1115
MalI GATC 2 cut(s) 277, 495
MbiI CCGCTC 1 cut(s) 244
MboI GATC 2 cut(s) 275, 493
MboII GAAGA 4 cut(s) 364, 367, 520, 966
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 5 cut(s) 65, 260, 266, 533, 1012
MlsI TGGCCA 1 cut(s) 89
MluCI AATT 4 cut(s) 67, 181, 952, 1157
MluNI TGGCCA 1 cut(s) 89
Mly113I GGCGCC 2 cut(s) 413, 520
MlyI GAGTC 2 cut(s) 323, 1067
MmeI TCCRAC 1 cut(s) 471
Mox20I TGGCCA 1 cut(s) 89
Mph1103I ATGCAT 1 cut(s) 1137
MroI TCCGGA 1 cut(s) 533
MroNI GCCGGC 2 cut(s) 455, 605
MscI TGGCCA 1 cut(s) 89
MseI TTAA 1 cut(s) 1089
MslI CAYNNNNRTG 2 cut(s) 56, 596
Msp20I TGGCCA 1 cut(s) 89
MspA1I CMGCKG 2 cut(s) 312, 477
MspI CCGG 6 cut(s) 10, 456, 527, 534, 606, 899
MspR9I CCNGG 5 cut(s) 10, 11, 528, 836, 899
MvaI CCWGG 1 cut(s) 836
MvnI CGCG 1 cut(s) 158
NaeI GCCGGC 2 cut(s) 457, 607
NarI GGCGCC 2 cut(s) 413, 520
NciI CCSGG 4 cut(s) 10, 11, 528, 899
NcoI CCATGG 1 cut(s) 84
NdeI CATATG 1 cut(s) 739
NdeII GATC 2 cut(s) 275, 493
NgoMIV GCCGGC 2 cut(s) 455, 605
NheI GCTAGC 1 cut(s) 1025
NlaIII CATG 7 cut(s) 55, 88, 256, 487, 590, 883, 1135
NmeAIII GCCGAG 3 cut(s) 131, 323, 413
NmuCI GTSAC 1 cut(s) 733
NotI GCGGCCGC 1 cut(s) 447
NsiI ATGCAT 1 cut(s) 1137
NspI RCATGY 1 cut(s) 883
PaeI GCATGC 1 cut(s) 883
PcsI WCGNNNNNNNCGW 1 cut(s) 284
PdiI GCCGGC 2 cut(s) 457, 607
PfeI GAWTC 4 cut(s) 5, 248, 424, 470
PleI GAGTC 2 cut(s) 323, 1066
PluTI GGCGCC 2 cut(s) 416, 523
PpsI GAGTC 2 cut(s) 323, 1066
Psp6I CCWGG 1 cut(s) 834
PspEI GGTNACC 1 cut(s) 800
PspFI CCCAGC 3 cut(s) 213, 859, 1166
PspGI CCWGG 1 cut(s) 834
PspOMI GGGCCC 1 cut(s) 61
PstNI CAGNNNCTG 1 cut(s) 845
PsuI RGATCY 1 cut(s) 493
RsaI GTAC 4 cut(s) 380, 730, 903, 1151
RsaNI GTAC 4 cut(s) 379, 729, 902, 1150
RseI CAYNNNNRTG 2 cut(s) 56, 596
SaqAI TTAA 1 cut(s) 1089
Sau3AI GATC 2 cut(s) 275, 493
ScaI AGTACT 1 cut(s) 380
SchI GAGTC 2 cut(s) 323, 1067
ScrFI CCNGG 5 cut(s) 10, 11, 528, 836, 899
SduI GDGCHC 5 cut(s) 65, 260, 266, 533, 1012
SetI ASST 9 cut(s) 549, 565, 630, 909, 923, 950, 1015, 1027, 1105
SfaNI GCATC 3 cut(s) 236, 333, 629
SfoI GGCGCC 2 cut(s) 414, 521
SinI GGWCC 2 cut(s) 650, 683
SmaI CCCGGG 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 56, 596
SmlI CTYRAG 1 cut(s) 1038
SmoI CTYRAG 1 cut(s) 1038
SphI GCATGC 1 cut(s) 883
Sse9I AATT 4 cut(s) 67, 181, 952, 1157
SspDI GGCGCC 2 cut(s) 412, 519
SspMI CTAG 3 cut(s) 945, 1026, 1122
StyD4I CCNGG 5 cut(s) 8, 9, 526, 834, 897
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 5 cut(s) 208, 661, 733, 1115, 1182
TaqI TCGA 6 cut(s) 230, 246, 270, 327, 549, 748
TasI AATT 4 cut(s) 67, 181, 952, 1157
TatI WGTACW 1 cut(s) 378
TfiI GAWTC 4 cut(s) 5, 248, 424, 470
Tru1I TTAA 1 cut(s) 1089
Tru9I TTAA 1 cut(s) 1089
TscAI CASTG 1 cut(s) 861
TseFI GTSAC 1 cut(s) 733
Tsp45I GTSAC 1 cut(s) 733
TspGWI ACGGA 2 cut(s) 288, 480
TspMI CCCGGG 1 cut(s) 9
TspRI CASTG 1 cut(s) 861
VneI GTGCAC 1 cut(s) 1008
VpaK11BI GGWCC 2 cut(s) 650, 683
XapI RAATTY 1 cut(s) 181
XceI RCATGY 1 cut(s) 883
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
XmaI CCCGGG 1 cut(s) 9
XspI CTAG 3 cut(s) 945, 1026, 1122
ZrmI AGTACT 1 cut(s) 380
Zsp2I ATGCAT 1 cut(s) 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.