Rmu_sc0000266.1_g000012

3-oxoacyl- acyl-carrier-protein synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000266.1
OG0004673
Physical Location & Seq
Reverse (-)
59071 .. 71265
12195 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1485 bp
atgaaatccgcttgctacaaaaaggtcaatgacttcaacaaggacaagttgcgcctaaaggcaagtctaaatttctgtggaaagggactcacagaagatgatatgatccagaagactctttccacttttcctacttcagcaattatactagctaaccagtataggctggagtatgataacaaaagaatcacaactttcaataagctaatcaacctactgcaagtggctgagaggcataatgagattcttttgagcaacaatgtcaggcccgttgggacaaagaaaattcctgaagctaattatggcaaagtcaaaggtggaaagaacctcaatgcaaagggggttggatgtgctgatcctcacccacatggcaacaacgcaccacgtggcaagggacgtgggggtcgtggtatgggccgtggaggccctcccaatgtatggcgcagagatggtggtgctggccctagtggtcatggaaacaaggtgcaaagggcacctaagaacccttcagtcaaacatggtagagttgttccgagcctgaggaggaaggttcagccttcaatagccatttttggatctgggtttctctcccctgagggaatctccaggagggtagtgctctgctccagcgctgagaagcaagctggagctacttcctcttctgaatctagagcacccaggcttgtcagtaaaggctgcaagttaatcgggtgtggctcggcagtacccagtcttaagatttcaaatgacgagctttcaaaaatagtagacactaacgatgaatggatatctgttcgcactgggattcgtaatcgacgagttatttcaggcaaagaaaacctaaccttattagcatctgaggcagcaaagaaagctcttgagatggcacaggttgatgctgatgatgtagacctaatcttgatgtgcacctctacacctgatgatctttttggcggggctcctcaggtacaaaaagcacttggctgcaaaaagcaccccttgtcctatgatattacagctgcctgtagtggattcgtgttgggtttagtatcagctgcttgtcacattaggggaggtggattcaaaaatgttctagttattggggctgatgctctttctcggattgttgactggtccgacagaggaacctgtattctctttggggatgctgccggggctgttttaatacaggccactgacactgaggaggatttcttacttggttttgacgttcatagtgatggtgacggaaacagacatctaaatgctccattgaaaggaaatgaaacagatagtgaattgggttctaatggttcagtcttagacttccctccaagacgctcttcattttcttgcgttcaaatgaatggaaaagaagtctttcggtttgcatgtcgatgtgtgccacaatcaattgagaattcacttgcgaatgctggtattcctgcatctagcattgactggttactgctccatcaggtatga

Protein Analysis

494

Amino Acids

52.85

Weight (kDa)

9.35

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACP_syn_III PF08545 336 - 415 4.7e-28 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 402
AccB1I GGYRCC 1 cut(s) 493
AccB7I CCANNNNNTGG 1 cut(s) 438
AccI GTMKAC 2 cut(s) 768, 909
AciI CCGC 2 cut(s) 9, 954
AclWI GGATC 3 cut(s) 100, 350, 583
AcsI RAATTY 3 cut(s) 70, 285, 1420
AcuI CTGAAG 3 cut(s) 120, 312, 492
AcvI CACGTG 1 cut(s) 386
AdeI CACNNNGTG 1 cut(s) 386
AfaI GTAC 2 cut(s) 726, 969
AfeI AGCGCT 1 cut(s) 631
AfiI CCNNNNNNNGG 2 cut(s) 438, 1277
AflII CTTAAG 1 cut(s) 734
AgsI TTSAA 8 cut(s) 37, 199, 561, 744, 759, 1084, 1276, 1361
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 2 cut(s) 605, 677
AjuI GAANNNNNNNTTGG 2 cut(s) 1327, 1359
AluBI AGCT 9 cut(s) 152, 205, 296, 644, 650, 754, 875, 1019, 1055
AluI AGCT 9 cut(s) 152, 205, 296, 644, 650, 754, 875, 1019, 1055
Alw21I GWGCWC 3 cut(s) 621, 676, 929
Alw44I GTGCAC 1 cut(s) 925
AlwI GGATC 3 cut(s) 100, 350, 583
Aor51HI AGCGCT 1 cut(s) 631
AoxI GGCC 5 cut(s) 266, 415, 424, 460, 1191
ApaLI GTGCAC 1 cut(s) 925
ApeKI GCWGC 6 cut(s) 696, 863, 984, 1019, 1055, 1169
ApoI RAATTY 3 cut(s) 70, 285, 1420
Asp700I GAANNNNTTC 1 cut(s) 1380
AspLEI GCGC 3 cut(s) 54, 444, 632
AspS9I GGNCC 5 cut(s) 267, 415, 425, 461, 1134
AsuC2I CCSGG 1 cut(s) 1174
AsuHPI GGTGA 2 cut(s) 353, 1256
AvaII GGWCC 1 cut(s) 1134
AxyI CCTNAGG 3 cut(s) 539, 594, 963
BaeGI GKGCMC 2 cut(s) 496, 929
BanI GGYRCC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 961
BbrPI CACGTG 1 cut(s) 386
BbsI GAAGAC 1 cut(s) 119
Bbv12I GWGCWC 3 cut(s) 621, 676, 929
BbvI GCAGC 6 cut(s) 683, 875, 971, 1006, 1042, 1156
BccI CCATC 4 cut(s) 443, 877, 1235, 1482
BceAI ACGGC 1 cut(s) 402
BcgI CGANNNNNNTGC 2 cut(s) 688, 722
BciT130I CCWGG 2 cut(s) 607, 679
BcnI CCSGG 1 cut(s) 1174
BfaI CTAG 5 cut(s) 149, 465, 669, 1094, 1452
BfmI CTRYAG 1 cut(s) 1024
BfoI RGCGCY 1 cut(s) 633
BfrI CTTAAG 1 cut(s) 734
BglI GCCNNNNNGGC 1 cut(s) 423
BisI GCNGC 6 cut(s) 697, 864, 985, 1020, 1056, 1170
BlsI GCNGC 6 cut(s) 698, 865, 986, 1021, 1057, 1171
Bme1390I CCNGG 3 cut(s) 607, 679, 1174
Bme18I GGWCC 1 cut(s) 1134
BmgBI CACGTC 1 cut(s) 398
BmgT120I GGNCC 5 cut(s) 267, 415, 425, 461, 1134
BmiI GGNNCC 3 cut(s) 495, 960, 1147
BmrFI CCNGG 3 cut(s) 607, 679, 1174
BmrI ACTGGG 2 cut(s) 723, 810
BmsI GCATC 5 cut(s) 863, 886, 1099, 1156, 1457
BmuI ACTGGG 2 cut(s) 723, 810
BpiI GAAGAC 1 cut(s) 119
BplI GAGNNNNNCTC 2 cut(s) 587, 619
BpmI CTGGAG 4 cut(s) 188, 589, 610, 666
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 1 cut(s) 1174
BsaAI YACGTR 1 cut(s) 386
BsaJI CCNNGG 3 cut(s) 418, 677, 1173
Bsc4I CCNNNNNNNGG 2 cut(s) 438, 1277
Bse1I ACTGG 5 cut(s) 157, 729, 805, 1136, 1466
Bse21I CCTNAGG 3 cut(s) 539, 594, 963
BseBI CCWGG 2 cut(s) 607, 679
BseDI CCNNGG 3 cut(s) 418, 677, 1173
BseGI GGATG 2 cut(s) 353, 1171
BseLI CCNNNNNNNGG 2 cut(s) 438, 1277
BseMII CTCAG 7 cut(s) 219, 530, 585, 624, 849, 977, 1194
BseNI ACTGG 5 cut(s) 157, 729, 805, 1136, 1466
BseRI GAGGAG 3 cut(s) 556, 951, 1220
BseSI GKGCMC 2 cut(s) 496, 929
BseXI GCAGC 6 cut(s) 683, 875, 971, 1006, 1042, 1156
BshFI GGCC 5 cut(s) 268, 417, 426, 462, 1193
BshNI GGYRCC 1 cut(s) 493
BsiHKAI GWGCWC 3 cut(s) 621, 676, 929
BsiSI CCGG 1 cut(s) 1173
BslFI GGGAC 3 cut(s) 99, 289, 408
BslI CCNNNNNNNGG 2 cut(s) 438, 1277
BsmFI GGGAC 3 cut(s) 99, 289, 408
BsmI GAATGC 1 cut(s) 1438
BsnI GGCC 5 cut(s) 268, 417, 426, 462, 1193
Bsp1286I GDGCHC 5 cut(s) 496, 621, 676, 929, 961
Bsp143I GATC 4 cut(s) 105, 355, 575, 943
BspACI CCGC 2 cut(s) 9, 954
BspANI GGCC 5 cut(s) 268, 417, 426, 462, 1193
BspCNI CTCAG 7 cut(s) 220, 531, 586, 625, 850, 976, 1195
BspLI GGNNCC 3 cut(s) 495, 960, 1147
BspPI GGATC 3 cut(s) 100, 350, 583
BspQI GCTCTTC 1 cut(s) 1348
BspT107I GGYRCC 1 cut(s) 493
BspTI CTTAAG 1 cut(s) 734
BsrI ACTGG 5 cut(s) 157, 729, 805, 1136, 1466
BssECI CCNNGG 3 cut(s) 418, 677, 1173
BssMI GATC 4 cut(s) 105, 355, 575, 943
Bst2UI CCWGG 2 cut(s) 607, 679
Bst6I CTCTTC 2 cut(s) 664, 1348
BstAFI CTTAAG 1 cut(s) 734
BstBAI YACGTR 1 cut(s) 386
BstC8I GCNNGC 3 cut(s) 13, 460, 642
BstDEI CTNAG 9 cut(s) 228, 498, 539, 594, 633, 858, 963, 1203, 1321
BstDSI CCRYGG 1 cut(s) 418
BstF5I GGATG 2 cut(s) 353, 1171
BstH2I RGCGCY 1 cut(s) 633
BstHHI GCGC 3 cut(s) 54, 444, 632
BstKTI GATC 4 cut(s) 108, 358, 578, 946
BstMBI GATC 4 cut(s) 105, 355, 575, 943
BstMWI GCNNNNNNNGC 4 cut(s) 423, 860, 872, 1175
BstNI CCWGG 2 cut(s) 607, 679
BstNSI RCATGY 1 cut(s) 1395
BstSCI CCNGG 3 cut(s) 605, 677, 1172
BstSFI CTRYAG 1 cut(s) 1024
BstSLI GKGCMC 2 cut(s) 496, 929
BstV1I GCAGC 6 cut(s) 683, 875, 971, 1006, 1042, 1156
BstV2I GAAGAC 1 cut(s) 119
BstX2I RGATCY 1 cut(s) 575
BstYI RGATCY 1 cut(s) 575
Bsu36I CCTNAGG 3 cut(s) 539, 594, 963
BsuRI GGCC 5 cut(s) 268, 417, 426, 462, 1193
BtgI CCRYGG 1 cut(s) 418
BtrI CACGTC 1 cut(s) 398
BtsCI GGATG 2 cut(s) 353, 1171
BtsIMutI CAGTG 3 cut(s) 798, 1194, 1200
Cac8I GCNNGC 3 cut(s) 13, 460, 642
CfoI GCGC 3 cut(s) 54, 444, 632
Cfr13I GGNCC 5 cut(s) 267, 415, 425, 461, 1134
CseI GACGC 1 cut(s) 1347
Csp6I GTAC 2 cut(s) 725, 968
CspCI CAANNNNNGTGG 2 cut(s) 379, 414
CviAII CATG 4 cut(s) 368, 473, 518, 1392
CviQI GTAC 2 cut(s) 725, 968
DdeI CTNAG 9 cut(s) 228, 498, 539, 594, 633, 858, 963, 1203, 1321
DpnI GATC 4 cut(s) 107, 357, 577, 945
DpnII GATC 4 cut(s) 105, 355, 575, 943
DraIII CACNNNGTG 1 cut(s) 386
DrdI GACNNNNNNGTC 1 cut(s) 402
DseDI GACNNNNNNGTC 1 cut(s) 402
Eam1104I CTCTTC 2 cut(s) 664, 1348
EarI CTCTTC 2 cut(s) 664, 1348
Eco24I GRGCYC 1 cut(s) 961
Eco32I GATATC 1 cut(s) 789
Eco47I GGWCC 1 cut(s) 1134
Eco47III AGCGCT 1 cut(s) 631
Eco57I CTGAAG 3 cut(s) 120, 312, 492
Eco72I CACGTG 1 cut(s) 386
Eco81I CCTNAGG 3 cut(s) 539, 594, 963
EcoO109I RGGNCCY 1 cut(s) 425
EcoRI GAATTC 1 cut(s) 1420
EcoRII CCWGG 2 cut(s) 605, 677
EcoRV GATATC 1 cut(s) 789
EcoT38I GRGCYC 1 cut(s) 961
FaeI CATG 4 cut(s) 371, 476, 521, 1395
FalI AAGNNNNNCTT 4 cut(s) 983, 1015, 1327, 1359
FaqI GGGAC 3 cut(s) 99, 289, 408
FatI CATG 4 cut(s) 367, 472, 517, 1391
FauI CCCGC 1 cut(s) 947
FblI GTMKAC 2 cut(s) 768, 909
Fnu4HI GCNGC 6 cut(s) 697, 864, 985, 1020, 1056, 1170
FokI GGATG 2 cut(s) 360, 1178
FriOI GRGCYC 1 cut(s) 961
Fsp4HI GCNGC 6 cut(s) 697, 864, 985, 1020, 1056, 1170
FspBI CTAG 5 cut(s) 149, 465, 669, 1094, 1452
GlaI GCGC 3 cut(s) 53, 443, 631
GluI GCNGC 6 cut(s) 697, 864, 985, 1020, 1056, 1170
GsuI CTGGAG 4 cut(s) 188, 589, 610, 666
HaeII RGCGCY 1 cut(s) 633
HaeIII GGCC 5 cut(s) 268, 417, 426, 462, 1193
HapII CCGG 1 cut(s) 1173
HgaI GACGC 1 cut(s) 1347
HhaI GCGC 3 cut(s) 54, 444, 632
Hin1II CATG 4 cut(s) 371, 476, 521, 1395
Hin6I GCGC 3 cut(s) 52, 442, 630
HinP1I GCGC 3 cut(s) 52, 442, 630
HincII GTYRAC 1 cut(s) 1129
HindII GTYRAC 1 cut(s) 1129
HinfI GANTC 9 cut(s) 87, 115, 186, 244, 600, 665, 805, 1032, 1080
HpaII CCGG 1 cut(s) 1173
HphI GGTGA 2 cut(s) 353, 1256
Hpy166II GTNNAC 4 cut(s) 769, 910, 927, 1129
Hpy188I TCNGA 5 cut(s) 534, 664, 859, 1122, 1138
Hpy188III TCNNGA 5 cut(s) 109, 290, 669, 878, 919
Hpy8I GTNNAC 4 cut(s) 769, 910, 927, 1129
Hpy99I CGWCG 1 cut(s) 819
HpyAV CCTTC 3 cut(s) 516, 541, 567
HpyCH4IV ACGT 3 cut(s) 385, 397, 1230
HpyCH4V TGCA 8 cut(s) 220, 335, 487, 699, 927, 987, 1391, 1448
HpyF10VI GCNNNNNNNGC 4 cut(s) 423, 860, 872, 1175
HpyF3I CTNAG 9 cut(s) 228, 498, 539, 594, 633, 858, 963, 1203, 1321
HpySE526I ACGT 3 cut(s) 385, 397, 1230
Hsp92II CATG 4 cut(s) 371, 476, 521, 1395
HspAI GCGC 3 cut(s) 52, 442, 630
Kzo9I GATC 4 cut(s) 105, 355, 575, 943
LguI GCTCTTC 1 cut(s) 1348
LmnI GCTCC 5 cut(s) 629, 647, 964, 1273, 1476
Lsp1109I GCAGC 6 cut(s) 683, 875, 971, 1006, 1042, 1156
LweI GCATC 5 cut(s) 863, 886, 1099, 1156, 1457
MaeI CTAG 5 cut(s) 149, 465, 669, 1094, 1452
MaeII ACGT 3 cut(s) 385, 397, 1230
MaeIII GTNAC 3 cut(s) 1061, 1244, 1464
MalI GATC 4 cut(s) 107, 357, 577, 945
MboI GATC 4 cut(s) 105, 355, 575, 943
MboII GAAGA 4 cut(s) 107, 124, 651, 1335
MfeI CAATTG 1 cut(s) 1413
MflI RGATCY 1 cut(s) 575
MhlI GDGCHC 5 cut(s) 496, 621, 676, 929, 961
MluCI AATT 7 cut(s) 70, 141, 285, 298, 1298, 1413, 1420
MlyI GAGTC 2 cut(s) 81, 109
MmeI TCCRAC 2 cut(s) 325, 1161
MroXI GAANNNNTTC 1 cut(s) 1380
MseI TTAA 3 cut(s) 704, 735, 1184
MslI CAYNNNNRTG 4 cut(s) 366, 1239, 1263, 1396
MspA1I CMGCKG 2 cut(s) 1019, 1055
MspCI CTTAAG 1 cut(s) 734
MspI CCGG 1 cut(s) 1173
MspR9I CCNGG 3 cut(s) 607, 679, 1174
MunI CAATTG 1 cut(s) 1413
Mva1269I GAATGC 1 cut(s) 1438
MvaI CCWGG 2 cut(s) 607, 679
MwoI GCNNNNNNNGC 4 cut(s) 423, 860, 872, 1175
NciI CCSGG 1 cut(s) 1174
NdeII GATC 4 cut(s) 105, 355, 575, 943
NlaIII CATG 4 cut(s) 371, 476, 521, 1395
NlaIV GGNNCC 3 cut(s) 495, 960, 1147
NmeAIII GCCGAG 1 cut(s) 698
NmuCI GTSAC 2 cut(s) 1061, 1244
NspI RCATGY 1 cut(s) 1395
PciSI GCTCTTC 1 cut(s) 1348
PcsI WCGNNNNNNNCGW 2 cut(s) 403, 814
PctI GAATGC 1 cut(s) 1438
PdmI GAANNNNTTC 1 cut(s) 1380
PfeI GAWTC 7 cut(s) 186, 244, 600, 665, 805, 1032, 1080
PflMI CCANNNNNTGG 1 cut(s) 438
PfoI TCCNGGA 1 cut(s) 605
PkrI GCNGC 6 cut(s) 698, 865, 986, 1021, 1057, 1171
PleI GAGTC 2 cut(s) 81, 109
PmaCI CACGTG 1 cut(s) 386
PmlI CACGTG 1 cut(s) 386
PpsI GAGTC 2 cut(s) 81, 109
Ppu21I YACGTR 1 cut(s) 386
Psp6I CCWGG 2 cut(s) 605, 677
PspCI CACGTG 1 cut(s) 386
PspGI CCWGG 2 cut(s) 605, 677
PspN4I GGNNCC 3 cut(s) 495, 960, 1147
PspPI GGNCC 5 cut(s) 267, 415, 425, 461, 1134
PsuI RGATCY 1 cut(s) 575
PvuII CAGCTG 2 cut(s) 1019, 1055
RsaI GTAC 2 cut(s) 726, 969
RsaNI GTAC 2 cut(s) 725, 968
RseI CAYNNNNRTG 4 cut(s) 366, 1239, 1263, 1396
SapI GCTCTTC 1 cut(s) 1348
SaqAI TTAA 3 cut(s) 704, 735, 1184
SatI GCNGC 6 cut(s) 697, 864, 985, 1020, 1056, 1170
Sau3AI GATC 4 cut(s) 105, 355, 575, 943
Sau96I GGNCC 5 cut(s) 267, 415, 425, 461, 1134
SchI GAGTC 2 cut(s) 81, 109
ScrFI CCNGG 3 cut(s) 607, 679, 1174
SduI GDGCHC 5 cut(s) 496, 621, 676, 929, 961
SfaNI GCATC 5 cut(s) 863, 886, 1099, 1156, 1457
SfcI CTRYAG 1 cut(s) 1024
SfiI GGCCNNNNNGGCC 1 cut(s) 423
SinI GGWCC 1 cut(s) 1134
SmiMI CAYNNNNRTG 4 cut(s) 366, 1239, 1263, 1396
SmlI CTYRAG 2 cut(s) 734, 878
SmoI CTYRAG 2 cut(s) 734, 878
Sse9I AATT 7 cut(s) 70, 141, 285, 298, 1298, 1413, 1420
SsiI CCGC 2 cut(s) 9, 954
SspMI CTAG 5 cut(s) 149, 465, 669, 1094, 1452
StyD4I CCNGG 3 cut(s) 605, 677, 1172
TaiI ACGT 3 cut(s) 388, 400, 1233
TaqI TCGA 2 cut(s) 814, 1396
TasI AATT 7 cut(s) 70, 141, 285, 298, 1298, 1413, 1420
TfiI GAWTC 7 cut(s) 186, 244, 600, 665, 805, 1032, 1080
Tru1I TTAA 3 cut(s) 704, 735, 1184
Tru9I TTAA 3 cut(s) 704, 735, 1184
TscAI CASTG 3 cut(s) 805, 1201, 1207
TseFI GTSAC 2 cut(s) 1061, 1244
TseI GCWGC 6 cut(s) 696, 863, 984, 1019, 1055, 1169
Tsp45I GTSAC 2 cut(s) 1061, 1244
TspDTI ATGAA 6 cut(s) 17, 795, 1223, 1299, 1335, 1379
TspGWI ACGGA 1 cut(s) 1263
TspRI CASTG 3 cut(s) 805, 1201, 1207
Van91I CCANNNNNTGG 1 cut(s) 438
Vha464I CTTAAG 1 cut(s) 734
VneI GTGCAC 1 cut(s) 925
VpaK11BI GGWCC 1 cut(s) 1134
XapI RAATTY 3 cut(s) 70, 285, 1420
XbaI TCTAGA 1 cut(s) 668
XceI RCATGY 1 cut(s) 1395
XmiI GTMKAC 2 cut(s) 768, 909
XmnI GAANNNNTTC 1 cut(s) 1380
XspI CTAG 5 cut(s) 149, 465, 669, 1094, 1452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.