Rorug07G0070300

3-oxoacyl- acyl-carrier-protein synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0004673
Physical Location & Seq
Forward (+)
5357412 .. 5361442
4031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0070300.1

Sequence Viewer

Length: 1491 bp
ATGGCCGCTAAACGCTTTTTAACTGATTCAGAATCCGACCAAGATCAGCCCAGTGAAAACAACCGGAAGAGACCAAGACTTTCCTTCGCTTCTGTGATTGGAGAAGTGGTTATGGTCAACTCCATGCAAAACCTTTTCTCATCCTTGGAGCCTTTGCTTAGAAGAGTGGTGAGTGAAGAGGTTGATCAAGTTCTAAGGCGGTGCTCTCGCTCTTTAACAAGGTCGCCTTCACTGAGAATCCAAGCGCTCGAGCCATCGAGCTTATATCTAGTCTTTGCCAAGAGCCTCTCTCTCCCAATTTTCACTGCAAGCAAGATATCAGATGAAGACAACAACCAAATGCAAATCCTTGCTGTGGACAAGAACATGATCAGTGGCAGCCACCAAATGGGTCGAATCTCTCCTCCACATCCTGTCAAAGTAGAAATCGTTGTTCTGGACGGTGACTTCCCTAGTGGAGATAGGGAGAATTGGACGAGTCAGGAATTCAACAACAATGTTTTGAAGGAGAGAACCGGCAAGAGGCCGTTGCTCGCCGGAGACGTGAATGTTACGGTGAGAGATGGATTTGCGACGATCGGGGACATTGAGTTTACTGACAATTCGAGTTGGATCCGGAGCAGGAAGTTTAGGCTTGGTGCAAGGGTGGCTCCTGGGATGGGGTATCAGGGTCCGAGGATTCGGGAAGCCATGACTGATGCTTTCGTTGTCAAAGATCATCGTGGAGAGTTGTACAAGAAGCATCATCCACCAATGTTGGAAGACGAAGTTTGGCGGCTGGAGAAGATTGGGAAAGATGGAGCTTTTCACAAAAAGCTAGCTGCGGACAACATTCATACAGTTCAAGATTTCTTGAAGATGTTTGTCGTCGACCGATCGAAGCTTAAAAAGATATTAGGGATTGGAATGTCAGAAAAGATGTGGGAAGTAACAGTGAAGCATGCAAGGACATGCGTGATGGGTAACAAAATGTACATTTTCCGGGGTCCTCATTTCGAGCTTCTCTTATCTCCAATATGTCAAGTGATGAAGGCTGTGATCAATGGCCAGACTTTTCCTGCTCGGGACCTAAGCAACTACAATAGGAACTATATTGAGAACTTGGTAAGGCAAGCGCATGCAAACTGGAATTCCTTGGAGGCGGTTGATACAATGTTGAATGAGAGTGCCTTACTTACACAAGGTCATGTAGAGGATCAGCACTATCCTCAGGCAATGATGATAAGAGCATTGGATCATCAAGTGATCAATAATGGGCAGCAGCAACAGCAACAGCAGCAGCAGCTAGCGGATATTAAGTGTATCAATAATGTTGGAGCTGCTGGTTATCCACAGAGCAACACCATGATGGCGTTTGAGTGCAGTGATAATTGGCAGATCAGTTCACCATACTCGTCGTTCAGCCCATTGATGGATAATGGTGTCATCAAGTGTAGCACCTCAGCAGACTCATCATCTTCAGATGGGGATTTAGGATCTCAGGGTCTCTAA

Protein Analysis

496

Amino Acids

55.81

Weight (kDa)

7.22

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 89 - 240 3.3e-57 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 252 - 318 1.3e-33 Calmodulin binding protein central domain
Calmod_bind_C PF20452 323 - 381 2e-19 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 388
AccI GTMKAC 1 cut(s) 870
AccIII TCCGGA 1 cut(s) 615
AciI CCGC 6 cut(s) 6, 199, 775, 824, 1142, 1289
AclWI GGATC 5 cut(s) 607, 620, 1203, 1242, 1483
AcoI YGGCCR 2 cut(s) 3, 1045
AcsI RAATTY 2 cut(s) 485, 1129
AcuI CTGAAG 1 cut(s) 1443
AfaI GTAC 2 cut(s) 734, 974
AfeI AGCGCT 1 cut(s) 246
AfiI CCNNNNNNNGG 5 cut(s) 355, 388, 522, 659, 1411
AgsI TTSAA 5 cut(s) 490, 505, 845, 856, 1159
AjiI CACGTC 1 cut(s) 544
AjnI CCWGG 1 cut(s) 652
AluBI AGCT 8 cut(s) 261, 803, 817, 821, 883, 1000, 1285, 1319
AluI AGCT 8 cut(s) 261, 803, 817, 821, 883, 1000, 1285, 1319
Alw21I GWGCWC 1 cut(s) 206
Alw26I GTCTC 2 cut(s) 64, 534
AlwI GGATC 5 cut(s) 607, 620, 1203, 1242, 1483
Ama87I CYCGRG 2 cut(s) 248, 1062
Aor13HI TCCGGA 1 cut(s) 615
Aor51HI AGCGCT 1 cut(s) 246
AoxI GGCC 3 cut(s) 3, 524, 1045
ApeKI GCWGC 8 cut(s) 378, 821, 1258, 1261, 1276, 1279, 1282, 1319
ApoI RAATTY 2 cut(s) 485, 1129
AspLEI GCGC 2 cut(s) 247, 1117
AspS9I GGNCC 3 cut(s) 671, 986, 1066
AsuC2I CCSGG 1 cut(s) 983
AsuHPI GGTGA 4 cut(s) 181, 455, 568, 1377
AsuNHI GCTAGC 2 cut(s) 817, 1285
AvaI CYCGRG 2 cut(s) 248, 1062
AvaII GGWCC 3 cut(s) 671, 986, 1066
AxyI CCTNAGG 1 cut(s) 1209
BalI TGGCCA 1 cut(s) 1047
BamHI GGATCC 1 cut(s) 612
BbsI GAAGAC 2 cut(s) 333, 768
Bbv12I GWGCWC 1 cut(s) 206
BbvCI CCTCAGC 1 cut(s) 1441
BbvI GCAGC 8 cut(s) 390, 808, 1270, 1273, 1288, 1291, 1294, 1306
BccI CCATC 8 cut(s) 262, 557, 652, 791, 952, 1342, 1405, 1457
BceAI ACGGC 1 cut(s) 511
BciT130I CCWGG 1 cut(s) 654
BclI TGATCA 4 cut(s) 184, 369, 1038, 1245
BcnI CCSGG 1 cut(s) 983
BcoDI GTCTC 2 cut(s) 64, 534
BfaI CTAG 4 cut(s) 269, 453, 818, 1286
BfoI RGCGCY 1 cut(s) 248
Bme1390I CCNGG 2 cut(s) 654, 983
Bme18I GGWCC 3 cut(s) 671, 986, 1066
BmeT110I CYCGRG 2 cut(s) 248, 1062
BmgBI CACGTC 1 cut(s) 544
BmgT120I GGNCC 3 cut(s) 671, 986, 1066
BmiI GGNNCC 6 cut(s) 150, 614, 651, 672, 987, 1067
BmrFI CCNGG 2 cut(s) 654, 983
BmrI ACTGGG 1 cut(s) 45
BmsI GCATC 2 cut(s) 688, 751
BmtI GCTAGC 2 cut(s) 821, 1289
BmuI ACTGGG 1 cut(s) 45
BpiI GAAGAC 2 cut(s) 333, 768
BplI GAGNNNNNCTC 2 cut(s) 274, 306
BpmI CTGGAG 1 cut(s) 800
Bpu10I CCTNAGC 2 cut(s) 1070, 1441
BpuMI CCSGG 1 cut(s) 983
BsaI GGTCTC 1 cut(s) 64
BsaJI CCNNGG 5 cut(s) 144, 653, 674, 982, 1134
BsaWI WCCGGW 2 cut(s) 63, 615
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bsc4I CCNNNNNNNGG 5 cut(s) 355, 388, 522, 659, 1411
Bse118I RCCGGY 1 cut(s) 515
Bse1I ACTGG 2 cut(s) 51, 1130
Bse21I CCTNAGG 1 cut(s) 1209
Bse3DI GCAATG 1 cut(s) 1221
BseAI TCCGGA 1 cut(s) 615
BseBI CCWGG 1 cut(s) 654
BseDI CCNNGG 5 cut(s) 144, 653, 674, 982, 1134
BseGI GGATG 4 cut(s) 140, 409, 663, 745
BseLI CCNNNNNNNGG 5 cut(s) 355, 388, 522, 659, 1411
BseMI GCAATG 1 cut(s) 1221
BseMII CTCAG 3 cut(s) 224, 1223, 1455
BseNI ACTGG 2 cut(s) 51, 1130
BseRI GAGGAG 1 cut(s) 393
BseXI GCAGC 8 cut(s) 390, 808, 1270, 1273, 1288, 1291, 1294, 1306
BsgI GTGCAG 1 cut(s) 1381
Bsh1285I CGRYCG 3 cut(s) 579, 874, 878
BshFI GGCC 3 cut(s) 5, 526, 1047
BsiEI CGRYCG 3 cut(s) 579, 874, 878
BsiHKAI GWGCWC 1 cut(s) 206
BsiHKCI CYCGRG 2 cut(s) 248, 1062
BsiSI CCGG 5 cut(s) 64, 516, 537, 616, 982
BslFI GGGAC 2 cut(s) 596, 1079
BslI CCNNNNNNNGG 5 cut(s) 355, 388, 522, 659, 1411
BsmAI GTCTC 2 cut(s) 64, 534
BsmBI CGTCTC 1 cut(s) 534
BsmFI GGGAC 2 cut(s) 596, 1079
BsnI GGCC 3 cut(s) 5, 526, 1047
Bso31I GGTCTC 1 cut(s) 64
BsoBI CYCGRG 2 cut(s) 248, 1062
Bsp1286I GDGCHC 1 cut(s) 206
Bsp13I TCCGGA 1 cut(s) 615
Bsp1407I TGTACA 2 cut(s) 732, 972
BspACI CCGC 6 cut(s) 6, 199, 775, 824, 1142, 1289
BspANI GGCC 3 cut(s) 5, 526, 1047
BspCNI CTCAG 3 cut(s) 225, 1222, 1454
BspEI TCCGGA 1 cut(s) 615
BspLI GGNNCC 6 cut(s) 150, 614, 651, 672, 987, 1067
BspOI GCTAGC 2 cut(s) 821, 1289
BspPI GGATC 5 cut(s) 607, 620, 1203, 1242, 1483
BspTNI GGTCTC 1 cut(s) 64
BsrDI GCAATG 1 cut(s) 1221
BsrFI RCCGGY 1 cut(s) 515
BsrGI TGTACA 2 cut(s) 732, 972
BsrI ACTGG 2 cut(s) 51, 1130
BssAI RCCGGY 1 cut(s) 515
BssECI CCNNGG 5 cut(s) 144, 653, 674, 982, 1134
BssT1I CCWWGG 2 cut(s) 144, 1134
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 4 cut(s) 443, 556, 841, 934
Bst6I CTCTTC 3 cut(s) 62, 157, 171
BstAUI TGTACA 2 cut(s) 732, 972
BstC8I GCNNGC 7 cut(s) 310, 534, 819, 942, 1113, 1119, 1287
BstDEI CTNAG 7 cut(s) 158, 194, 233, 1070, 1209, 1441, 1479
BstF5I GGATG 4 cut(s) 140, 409, 663, 745
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 2 cut(s) 247, 1117
BstMAI GTCTC 2 cut(s) 64, 534
BstMCI CGRYCG 3 cut(s) 579, 874, 878
BstMWI GCNNNNNNNGC 4 cut(s) 647, 1267, 1276, 1282
BstNI CCWGG 1 cut(s) 654
BstNSI RCATGY 3 cut(s) 944, 954, 1121
BstSCI CCNGG 2 cut(s) 652, 981
BstV1I GCAGC 8 cut(s) 390, 808, 1270, 1273, 1288, 1291, 1294, 1306
BstV2I GAAGAC 2 cut(s) 333, 768
BstX2I RGATCY 2 cut(s) 612, 1475
BstYI RGATCY 2 cut(s) 612, 1475
Bsu36I CCTNAGG 1 cut(s) 1209
BsuRI GGCC 3 cut(s) 5, 526, 1047
BtrI CACGTC 1 cut(s) 544
BtsCI GGATG 4 cut(s) 140, 409, 663, 745
BtsI GCAGTG 2 cut(s) 303, 1369
BtsIMutI CAGTG 6 cut(s) 58, 230, 303, 379, 939, 1369
Cac8I GCNNGC 7 cut(s) 310, 534, 819, 942, 1113, 1119, 1287
CfoI GCGC 2 cut(s) 247, 1117
Cfr10I RCCGGY 1 cut(s) 515
Cfr13I GGNCC 3 cut(s) 671, 986, 1066
Csp6I GTAC 2 cut(s) 733, 973
CspCI CAANNNNNGTGG 2 cut(s) 738, 773
CviAII CATG 8 cut(s) 124, 367, 691, 941, 951, 1118, 1187, 1345
CviQI GTAC 2 cut(s) 733, 973
DdeI CTNAG 7 cut(s) 158, 194, 233, 1070, 1209, 1441, 1479
EaeI YGGCCR 2 cut(s) 3, 1045
Eam1104I CTCTTC 3 cut(s) 62, 157, 171
EarI CTCTTC 3 cut(s) 62, 157, 171
Eco130I CCWWGG 2 cut(s) 144, 1134
Eco31I GGTCTC 1 cut(s) 64
Eco32I GATATC 1 cut(s) 318
Eco47I GGWCC 3 cut(s) 671, 986, 1066
Eco47III AGCGCT 1 cut(s) 246
Eco57I CTGAAG 1 cut(s) 1443
Eco81I CCTNAGG 1 cut(s) 1209
Eco88I CYCGRG 2 cut(s) 248, 1062
EcoO109I RGGNCCY 2 cut(s) 986, 1066
EcoRI GAATTC 2 cut(s) 485, 1129
EcoRII CCWGG 1 cut(s) 652
EcoRV GATATC 1 cut(s) 318
EcoT14I CCWWGG 2 cut(s) 144, 1134
ErhI CCWWGG 2 cut(s) 144, 1134
Esp3I CGTCTC 1 cut(s) 534
FaeI CATG 8 cut(s) 127, 370, 694, 944, 954, 1121, 1190, 1348
FalI AAGNNNNNCTT 2 cut(s) 211, 243
FaqI GGGAC 2 cut(s) 596, 1079
FatI CATG 8 cut(s) 123, 366, 690, 940, 950, 1117, 1186, 1344
FbaI TGATCA 4 cut(s) 184, 369, 1038, 1245
FblI GTMKAC 1 cut(s) 870
FokI GGATG 4 cut(s) 127, 396, 670, 732
FspBI CTAG 4 cut(s) 269, 453, 818, 1286
GlaI GCGC 2 cut(s) 246, 1116
GsuI CTGGAG 1 cut(s) 800
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 3 cut(s) 5, 526, 1047
HapII CCGG 5 cut(s) 64, 516, 537, 616, 982
HhaI GCGC 2 cut(s) 247, 1117
Hin1II CATG 8 cut(s) 127, 370, 694, 944, 954, 1121, 1190, 1348
Hin6I GCGC 2 cut(s) 245, 1115
HinP1I GCGC 2 cut(s) 245, 1115
HincII GTYRAC 2 cut(s) 118, 871
HindII GTYRAC 2 cut(s) 118, 871
HindIII AAGCTT 1 cut(s) 881
HinfI GANTC 7 cut(s) 26, 32, 237, 396, 478, 679, 1448
HpaII CCGG 5 cut(s) 64, 516, 537, 616, 982
HphI GGTGA 4 cut(s) 181, 455, 568, 1377
Hpy166II GTNNAC 5 cut(s) 118, 358, 594, 871, 1385
Hpy188I TCNGA 6 cut(s) 31, 37, 322, 675, 913, 1462
Hpy188III TCNNGA 7 cut(s) 437, 482, 616, 683, 845, 853, 1064
Hpy8I GTNNAC 5 cut(s) 118, 358, 594, 871, 1385
Hpy99I CGWCG 3 cut(s) 577, 872, 1399
HpyAV CCTTC 4 cut(s) 94, 237, 499, 1024
HpyCH4III ACNGT 4 cut(s) 443, 556, 841, 934
HpyCH4IV ACGT 1 cut(s) 543
HpyCH4V TGCA 7 cut(s) 127, 308, 343, 641, 944, 1121, 1362
HpyF10VI GCNNNNNNNGC 4 cut(s) 647, 1267, 1276, 1282
HpyF3I CTNAG 7 cut(s) 158, 194, 233, 1070, 1209, 1441, 1479
HpySE526I ACGT 1 cut(s) 543
Hsp92II CATG 8 cut(s) 127, 370, 694, 944, 954, 1121, 1190, 1348
HspAI GCGC 2 cut(s) 245, 1115
Kpn2I TCCGGA 1 cut(s) 615
Ksp22I TGATCA 4 cut(s) 184, 369, 1038, 1245
LmnI GCTCC 5 cut(s) 148, 618, 655, 800, 1316
Lsp1109I GCAGC 8 cut(s) 390, 808, 1270, 1273, 1288, 1291, 1294, 1306
LweI GCATC 2 cut(s) 688, 751
MaeI CTAG 4 cut(s) 269, 453, 818, 1286
MaeII ACGT 1 cut(s) 543
MaeIII GTNAC 4 cut(s) 443, 550, 928, 962
MboII GAAGA 8 cut(s) 79, 174, 188, 338, 773, 796, 868, 1449
MflI RGATCY 2 cut(s) 612, 1475
MhlI GDGCHC 1 cut(s) 206
MlsI TGGCCA 1 cut(s) 1047
MluCI AATT 6 cut(s) 297, 469, 485, 601, 1129, 1369
MluNI TGGCCA 1 cut(s) 1047
MlyI GAGTC 2 cut(s) 487, 1442
MmeI TCCRAC 4 cut(s) 60, 590, 738, 1294
Mox20I TGGCCA 1 cut(s) 1047
MroI TCCGGA 1 cut(s) 615
MscI TGGCCA 1 cut(s) 1047
MseI TTAA 4 cut(s) 20, 215, 885, 1296
MslI CAYNNNNRTG 1 cut(s) 1346
Msp20I TGGCCA 1 cut(s) 1047
MspI CCGG 5 cut(s) 64, 516, 537, 616, 982
MspR9I CCNGG 2 cut(s) 654, 983
MvaI CCWGG 1 cut(s) 654
MwoI GCNNNNNNNGC 4 cut(s) 647, 1267, 1276, 1282
NciI CCSGG 1 cut(s) 983
NheI GCTAGC 2 cut(s) 817, 1285
NlaIII CATG 8 cut(s) 127, 370, 694, 944, 954, 1121, 1190, 1348
NlaIV GGNNCC 6 cut(s) 150, 614, 651, 672, 987, 1067
NmuCI GTSAC 1 cut(s) 443
NspI RCATGY 3 cut(s) 944, 954, 1121
PaeI GCATGC 2 cut(s) 944, 1121
PaeR7I CTCGAG 1 cut(s) 248
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PfeI GAWTC 5 cut(s) 26, 32, 237, 396, 679
PflMI CCANNNNNTGG 1 cut(s) 388
Ple19I CGATCG 2 cut(s) 579, 878
PleI GAGTC 2 cut(s) 486, 1442
PpsI GAGTC 2 cut(s) 486, 1442
PpuMI RGGWCCY 2 cut(s) 986, 1066
Psp5II RGGWCCY 2 cut(s) 986, 1066
Psp6I CCWGG 1 cut(s) 652
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 6 cut(s) 150, 614, 651, 672, 987, 1067
PspPI GGNCC 3 cut(s) 671, 986, 1066
PspPPI RGGWCCY 2 cut(s) 986, 1066
PspXI VCTCGAGB 1 cut(s) 248
PsuI RGATCY 2 cut(s) 612, 1475
PvuI CGATCG 2 cut(s) 579, 878
RsaI GTAC 2 cut(s) 734, 974
RsaNI GTAC 2 cut(s) 733, 973
RseI CAYNNNNRTG 1 cut(s) 1346
SalI GTCGAC 1 cut(s) 869
SaqAI TTAA 4 cut(s) 20, 215, 885, 1296
Sau96I GGNCC 3 cut(s) 671, 986, 1066
SchI GAGTC 2 cut(s) 487, 1442
ScrFI CCNGG 2 cut(s) 654, 983
SduI GDGCHC 1 cut(s) 206
SfaNI GCATC 2 cut(s) 688, 751
Sfr274I CTCGAG 1 cut(s) 248
SinI GGWCC 3 cut(s) 671, 986, 1066
SlaI CTCGAG 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 1346
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
SphI GCATGC 2 cut(s) 944, 1121
Sse9I AATT 6 cut(s) 297, 469, 485, 601, 1129, 1369
SsiI CCGC 6 cut(s) 6, 199, 775, 824, 1142, 1289
SspMI CTAG 4 cut(s) 269, 453, 818, 1286
StyD4I CCNGG 2 cut(s) 652, 981
StyI CCWWGG 2 cut(s) 144, 1134
TaaI ACNGT 4 cut(s) 443, 556, 841, 934
TaiI ACGT 1 cut(s) 546
TaqI TCGA 7 cut(s) 249, 257, 394, 605, 870, 878, 996
TaqII GACCGA 1 cut(s) 888
TasI AATT 6 cut(s) 297, 469, 485, 601, 1129, 1369
TatI WGTACW 2 cut(s) 732, 972
TauI GCSGC 2 cut(s) 8, 778
TfiI GAWTC 5 cut(s) 26, 32, 237, 396, 679
Tru1I TTAA 4 cut(s) 20, 215, 885, 1296
Tru9I TTAA 4 cut(s) 20, 215, 885, 1296
TscAI CASTG 6 cut(s) 58, 237, 310, 379, 939, 1369
TseFI GTSAC 1 cut(s) 443
TseI GCWGC 8 cut(s) 378, 821, 1258, 1261, 1276, 1279, 1282, 1319
Tsp45I GTSAC 1 cut(s) 443
TspDTI ATGAA 3 cut(s) 339, 824, 1043
TspRI CASTG 6 cut(s) 58, 237, 310, 379, 939, 1369
Van91I CCANNNNNTGG 1 cut(s) 388
VpaK11BI GGWCC 3 cut(s) 671, 986, 1066
XapI RAATTY 2 cut(s) 485, 1129
XceI RCATGY 3 cut(s) 944, 954, 1121
XhoI CTCGAG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 870
XspI CTAG 4 cut(s) 269, 453, 818, 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.