Rmu_sc0000299.1_g000004

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000299.1
OG0016466
Physical Location & Seq
Reverse (-)
24157 .. 33722
9566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000299.1_g000004.1.cds

Sequence Viewer

Length: 765 bp
atggatggcgtagaggatggcggtggtcaggtagtggtggagaagtcagattcagttgtggtggtggaagagctagtagctatggatgaagaggcagcagtggtggtggaggcaagaaattgttcgagtaaggatggagagccatggagggagagtgatggtggtggaggtgagttgcggtggtgcgtagagaagtgtggtggaggagcggctcgagttctcatacagaaacccagaagcaaaatgggattgatttcatcaggaaatggggcacggaagatgaatttcggaagtccaagggtcgccgatgccggagggtaccagactgtagcaggcgattctcgacgtcggtcctggcttggtcgtcgttgctactccgggagaccggctcgctactttgtcgacgctaagcctgctccggtggttgctttgttttcgggtagaggaccgaacgggttgaacccggaaattctgaagcctgatctgatcgttccgggagtcaacatcctcgctgctaggaccgacgcggtgggctcaaccggattggaaacggataactggaaatatcctctcgtgaacatcaatggcttgatcaatggcttgccctcacgggccactctgctcaagtctctcccccccgacttttttttcgttcagctaccggtgaccggaatcaggcaatcggtggccggaatcctgcaaaagttggccggatcgagctccggcgaagtcccttatggtttctctcttccattctctctctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

254

Amino Acids

26.85

Weight (kDa)

8.33

Isoelectric Point (pI)

51.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 349
Acc65I GGTACC 1 cut(s) 318
AccB1I GGYRCC 1 cut(s) 318
AccBSI CCGCTC 1 cut(s) 209
AccI GTMKAC 1 cut(s) 402
AccII CGCG 1 cut(s) 527
AciI CCGC 4 cut(s) 21, 178, 209, 527
AclWI GGATC 1 cut(s) 721
AcoI YGGCCR 2 cut(s) 687, 708
AcsI RAATTY 2 cut(s) 283, 468
AcuI CTGAAG 1 cut(s) 494
AcyI GRCGYC 1 cut(s) 346
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 2 cut(s) 668, 675
AgeI ACCGGT 1 cut(s) 661
AgsI TTSAA 1 cut(s) 460
AjnI CCWGG 1 cut(s) 353
AluBI AGCT 4 cut(s) 73, 80, 658, 720
AluI AGCT 4 cut(s) 73, 80, 658, 720
Alw21I GWGCWC 1 cut(s) 722
Alw26I GTCTC 2 cut(s) 376, 633
AlwI GGATC 1 cut(s) 721
Ama87I CYCGRG 1 cut(s) 213
AoxI GGCC 3 cut(s) 612, 687, 708
ApeKI GCWGC 2 cut(s) 95, 512
ApoI RAATTY 2 cut(s) 283, 468
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AsiGI ACCGGT 1 cut(s) 661
Asp700I GAANNNNTTC 1 cut(s) 121
Asp718I GGTACC 1 cut(s) 318
AspS9I GGNCC 4 cut(s) 351, 446, 519, 612
AsuC2I CCSGG 3 cut(s) 379, 464, 495
AsuHPI GGTGA 2 cut(s) 182, 676
AvaI CYCGRG 1 cut(s) 213
AvaII GGWCC 3 cut(s) 351, 446, 519
BaeGI GKGCMC 1 cut(s) 274
BanI GGYRCC 1 cut(s) 318
BanII GRGCYC 2 cut(s) 536, 722
BauI CACGAG 1 cut(s) 572
Bbv12I GWGCWC 1 cut(s) 722
BbvI GCAGC 2 cut(s) 107, 499
BccI CCATC 3 cut(s) 11, 128, 152
BciT130I CCWGG 1 cut(s) 355
BclI TGATCA 1 cut(s) 591
BcnI CCSGG 3 cut(s) 379, 464, 495
BcoDI GTCTC 2 cut(s) 376, 633
BfaI CTAG 2 cut(s) 74, 516
BfmI CTRYAG 1 cut(s) 327
BisI GCNGC 3 cut(s) 96, 210, 513
BlpI GCTNAGC 1 cut(s) 408
BlsI GCNGC 3 cut(s) 97, 211, 514
Bme1390I CCNGG 4 cut(s) 355, 379, 464, 495
Bme18I GGWCC 3 cut(s) 351, 446, 519
BmeT110I CYCGRG 1 cut(s) 213
BmgT120I GGNCC 4 cut(s) 351, 446, 519, 612
BmiI GGNNCC 1 cut(s) 320
BmrFI CCNGG 4 cut(s) 355, 379, 464, 495
BmsI GCATC 1 cut(s) 298
BoxI GACNNNNGTC 1 cut(s) 349
BplI GAGNNNNNCTC 2 cut(s) 373, 405
Bpu1102I GCTNAGC 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 608
BpuMI CCSGG 3 cut(s) 379, 464, 495
BsaHI GRCGYC 1 cut(s) 346
BsaI GGTCTC 1 cut(s) 376
BsaJI CCNNGG 2 cut(s) 143, 296
BsaWI WCCGGW 4 cut(s) 418, 539, 661, 668
BsaXI ACNNNNNCTCC 2 cut(s) 139, 169
Bsc4I CCNNNNNNNGG 2 cut(s) 668, 675
Bse118I RCCGGY 2 cut(s) 385, 661
Bse1I ACTGG 1 cut(s) 563
BseBI CCWGG 1 cut(s) 355
BseDI CCNNGG 2 cut(s) 143, 296
BseGI GGATG 5 cut(s) 10, 22, 91, 139, 504
BseLI CCNNNNNNNGG 2 cut(s) 668, 675
BseNI ACTGG 1 cut(s) 563
BseRI GAGGAG 1 cut(s) 219
BseSI GKGCMC 1 cut(s) 274
BseXI GCAGC 2 cut(s) 107, 499
Bsh1236I CGCG 1 cut(s) 527
BshFI GGCC 3 cut(s) 614, 689, 710
BshNI GGYRCC 1 cut(s) 318
BshTI ACCGGT 1 cut(s) 661
BsiHKAI GWGCWC 1 cut(s) 722
BsiHKCI CYCGRG 1 cut(s) 213
BslFI GGGAC 1 cut(s) 716
BslI CCNNNNNNNGG 2 cut(s) 668, 675
BsmAI GTCTC 2 cut(s) 376, 633
BsmFI GGGAC 1 cut(s) 716
BsnI GGCC 3 cut(s) 614, 689, 710
Bso31I GGTCTC 1 cut(s) 376
BsoBI CYCGRG 1 cut(s) 213
Bsp1286I GDGCHC 3 cut(s) 274, 536, 722
Bsp143I GATC 4 cut(s) 481, 486, 591, 713
Bsp1720I GCTNAGC 1 cut(s) 408
Bsp19I CCATGG 1 cut(s) 143
BspACI CCGC 4 cut(s) 21, 178, 209, 527
BspANI GGCC 3 cut(s) 614, 689, 710
BspFNI CGCG 1 cut(s) 527
BspLI GGNNCC 1 cut(s) 320
BspPI GGATC 1 cut(s) 721
BspQI GCTCTTC 1 cut(s) 63
BspT107I GGYRCC 1 cut(s) 318
BspTNI GGTCTC 1 cut(s) 376
BsrBI CCGCTC 1 cut(s) 209
BsrFI RCCGGY 2 cut(s) 385, 661
BsrI ACTGG 1 cut(s) 563
BssAI RCCGGY 2 cut(s) 385, 661
BssECI CCNNGG 2 cut(s) 143, 296
BssMI GATC 4 cut(s) 481, 486, 591, 713
BssNI GRCGYC 1 cut(s) 346
BssSI CACGAG 1 cut(s) 572
BssT1I CCWWGG 2 cut(s) 143, 296
Bst2BI CACGAG 1 cut(s) 572
Bst2UI CCWGG 1 cut(s) 355
Bst4CI ACNGT 1 cut(s) 328
Bst6I CTCTTC 3 cut(s) 63, 84, 753
BstACI GRCGYC 1 cut(s) 346
BstC8I GCNNGC 4 cut(s) 334, 391, 414, 602
BstDEI CTNAG 1 cut(s) 408
BstDSI CCRYGG 1 cut(s) 143
BstEII GGTNACC 1 cut(s) 664
BstF5I GGATG 5 cut(s) 10, 22, 91, 139, 504
BstFNI CGCG 1 cut(s) 527
BstKTI GATC 4 cut(s) 484, 489, 594, 716
BstMAI GTCTC 2 cut(s) 376, 633
BstMBI GATC 4 cut(s) 481, 486, 591, 713
BstMWI GCNNNNNNNGC 1 cut(s) 413
BstNI CCWGG 1 cut(s) 355
BstPAI GACNNNNGTC 1 cut(s) 349
BstPI GGTNACC 1 cut(s) 664
BstSCI CCNGG 4 cut(s) 353, 377, 462, 493
BstSFI CTRYAG 1 cut(s) 327
BstSLI GKGCMC 1 cut(s) 274
BstUI CGCG 1 cut(s) 527
BstV1I GCAGC 2 cut(s) 107, 499
BsuRI GGCC 3 cut(s) 614, 689, 710
BtgI CCRYGG 1 cut(s) 143
BtsCI GGATG 5 cut(s) 10, 22, 91, 139, 504
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 1 cut(s) 105
Cac8I GCNNGC 4 cut(s) 334, 391, 414, 602
Cfr10I RCCGGY 2 cut(s) 385, 661
Cfr13I GGNCC 4 cut(s) 351, 446, 519, 612
CseI GACGC 2 cut(s) 413, 533
Csp6I GTAC 1 cut(s) 319
CspAI ACCGGT 1 cut(s) 661
CviAII CATG 1 cut(s) 144
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 1 cut(s) 408
DpnI GATC 4 cut(s) 483, 488, 593, 715
DpnII GATC 4 cut(s) 481, 486, 591, 713
EaeI YGGCCR 2 cut(s) 687, 708
Eam1104I CTCTTC 3 cut(s) 63, 84, 753
EarI CTCTTC 3 cut(s) 63, 84, 753
Ecl136II GAGCTC 1 cut(s) 720
Eco130I CCWWGG 2 cut(s) 143, 296
Eco24I GRGCYC 2 cut(s) 536, 722
Eco31I GGTCTC 1 cut(s) 376
Eco47I GGWCC 3 cut(s) 351, 446, 519
Eco53kI GAGCTC 1 cut(s) 720
Eco57I CTGAAG 1 cut(s) 494
Eco88I CYCGRG 1 cut(s) 213
Eco91I GGTNACC 1 cut(s) 664
EcoICRI GAGCTC 1 cut(s) 720
EcoO65I GGTNACC 1 cut(s) 664
EcoRII CCWGG 1 cut(s) 353
EcoT14I CCWWGG 2 cut(s) 143, 296
EcoT38I GRGCYC 2 cut(s) 536, 722
ErhI CCWWGG 2 cut(s) 143, 296
FaeI CATG 1 cut(s) 147
FaiI YATR 4 cut(s) 83, 145, 224, 738
FaqI GGGAC 1 cut(s) 716
FatI CATG 1 cut(s) 143
FbaI TGATCA 1 cut(s) 591
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 3 cut(s) 96, 210, 513
FokI GGATG 5 cut(s) 17, 29, 98, 146, 491
FriOI GRGCYC 2 cut(s) 536, 722
Fsp4HI GCNGC 3 cut(s) 96, 210, 513
FspBI CTAG 2 cut(s) 74, 516
GluI GCNGC 3 cut(s) 96, 210, 513
HaeIII GGCC 3 cut(s) 614, 689, 710
HgaI GACGC 2 cut(s) 413, 533
Hin1I GRCGYC 1 cut(s) 346
Hin1II CATG 1 cut(s) 147
HincII GTYRAC 2 cut(s) 403, 502
HindII GTYRAC 2 cut(s) 403, 502
HinfI GANTC 5 cut(s) 50, 338, 498, 672, 693
HphI GGTGA 2 cut(s) 182, 676
Hpy166II GTNNAC 3 cut(s) 403, 502, 577
Hpy188I TCNGA 4 cut(s) 49, 290, 474, 486
Hpy188III TCNNGA 3 cut(s) 261, 342, 574
Hpy8I GTNNAC 3 cut(s) 403, 502, 577
Hpy99I CGWCG 5 cut(s) 348, 351, 369, 407, 527
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4IV ACGT 1 cut(s) 346
HpyCH4V TGCA 1 cut(s) 700
HpyF10VI GCNNNNNNNGC 1 cut(s) 413
HpyF3I CTNAG 1 cut(s) 408
HpySE526I ACGT 1 cut(s) 346
Hsp92I GRCGYC 1 cut(s) 346
Hsp92II CATG 1 cut(s) 147
KpnI GGTACC 1 cut(s) 322
Ksp22I TGATCA 1 cut(s) 591
Kzo9I GATC 4 cut(s) 481, 486, 591, 713
LguI GCTCTTC 1 cut(s) 63
LmnI GCTCC 3 cut(s) 206, 421, 725
Lsp1109I GCAGC 2 cut(s) 107, 499
LweI GCATC 1 cut(s) 298
MaeI CTAG 2 cut(s) 74, 516
MaeII ACGT 1 cut(s) 346
MaeIII GTNAC 1 cut(s) 664
MalI GATC 4 cut(s) 483, 488, 593, 715
MbiI CCGCTC 1 cut(s) 209
MboI GATC 4 cut(s) 481, 486, 591, 713
MboII GAAGA 4 cut(s) 80, 101, 289, 740
MhlI GDGCHC 3 cut(s) 274, 536, 722
MluCI AATT 3 cut(s) 118, 283, 468
MlyI GAGTC 1 cut(s) 507
MroXI GAANNNNTTC 1 cut(s) 121
MspR9I CCNGG 4 cut(s) 355, 379, 464, 495
MvaI CCWGG 1 cut(s) 355
MvnI CGCG 1 cut(s) 527
MwoI GCNNNNNNNGC 1 cut(s) 413
NciI CCSGG 3 cut(s) 379, 464, 495
NcoI CCATGG 1 cut(s) 143
NdeII GATC 4 cut(s) 481, 486, 591, 713
NlaIII CATG 1 cut(s) 147
NlaIV GGNNCC 1 cut(s) 320
NmuCI GTSAC 1 cut(s) 664
PaeR7I CTCGAG 1 cut(s) 213
PciSI GCTCTTC 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 121
PfeI GAWTC 4 cut(s) 50, 338, 672, 693
PfoI TCCNGGA 2 cut(s) 377, 493
PinAI ACCGGT 1 cut(s) 661
PkrI GCNGC 3 cut(s) 97, 211, 514
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
PshAI GACNNNNGTC 1 cut(s) 349
Psp124BI GAGCTC 1 cut(s) 722
Psp6I CCWGG 1 cut(s) 353
PspEI GGTNACC 1 cut(s) 664
PspGI CCWGG 1 cut(s) 353
PspN4I GGNNCC 1 cut(s) 320
PspPI GGNCC 4 cut(s) 351, 446, 519, 612
PspXI VCTCGAGB 1 cut(s) 213
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
SacI GAGCTC 1 cut(s) 722
SalI GTCGAC 1 cut(s) 401
SapI GCTCTTC 1 cut(s) 63
SatI GCNGC 3 cut(s) 96, 210, 513
Sau3AI GATC 4 cut(s) 481, 486, 591, 713
Sau96I GGNCC 4 cut(s) 351, 446, 519, 612
SchI GAGTC 1 cut(s) 507
ScrFI CCNGG 4 cut(s) 355, 379, 464, 495
SduI GDGCHC 3 cut(s) 274, 536, 722
SetI ASST 7 cut(s) 33, 75, 82, 172, 349, 660, 722
SfaNI GCATC 1 cut(s) 298
SfcI CTRYAG 1 cut(s) 327
Sfr274I CTCGAG 1 cut(s) 213
SinI GGWCC 3 cut(s) 351, 446, 519
SlaI CTCGAG 1 cut(s) 213
SmlI CTYRAG 2 cut(s) 213, 623
SmoI CTYRAG 2 cut(s) 213, 623
Sse9I AATT 3 cut(s) 118, 283, 468
SsiI CCGC 4 cut(s) 21, 178, 209, 527
SspMI CTAG 2 cut(s) 74, 516
SstI GAGCTC 1 cut(s) 722
StyD4I CCNGG 4 cut(s) 353, 377, 462, 493
StyI CCWWGG 2 cut(s) 143, 296
TaaI ACNGT 1 cut(s) 328
TaiI ACGT 1 cut(s) 349
TaqI TCGA 5 cut(s) 125, 214, 343, 402, 716
TaqII GACCGA 3 cut(s) 339, 463, 536
TasI AATT 3 cut(s) 118, 283, 468
TauI GCSGC 1 cut(s) 212
TfiI GAWTC 4 cut(s) 50, 338, 672, 693
TscAI CASTG 1 cut(s) 105
TseFI GTSAC 1 cut(s) 664
TseI GCWGC 2 cut(s) 95, 512
Tsp45I GTSAC 1 cut(s) 664
TspDTI ATGAA 3 cut(s) 102, 246, 296
TspGWI ACGGA 2 cut(s) 289, 566
TspRI CASTG 1 cut(s) 105
VpaK11BI GGWCC 3 cut(s) 351, 446, 519
XapI RAATTY 2 cut(s) 283, 468
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XhoI CTCGAG 1 cut(s) 213
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 121
XspI CTAG 2 cut(s) 74, 516
ZraI GACGTC 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.