Rmu_sc0040042.1_g000001

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0040042.1
OG0016466
Physical Location & Seq
Reverse (-)
313 .. 1522
1210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0040042.1_g000001.1.cds

Sequence Viewer

Length: 405 bp
atgctgcttggtcgtcgttgctgctccgggagaccggctcgctactttgccgacgctaagcctgctccggtggttgctttgttttcgggtagaggaccgaacgggttgaacccggaaattctgaagcctgatctgatcgttccgggagtcaacatcctcgctgctaggaccgacgcggtgggcttaaccggattggaaacggataactggaaatatcctctcgtgaacatcaatggcttgccctcacgggccactcaaacacccccgacctttttttcgttcagaaattccggtgggcagctaccggtgaccggaatccggcaaacttggattccggattccggtaatccggcggccggtgaccgagctccggcgaagtcccttatggtttctctctctaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

134

Amino Acids

14.2

Weight (kDa)

10.13

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 176
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 2 cut(s) 176, 353
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 2 cut(s) 117, 286
AcuI CTGAAG 1 cut(s) 143
AfiI CCNNNNNNNGG 5 cut(s) 311, 318, 341, 356, 370
AgeI ACCGGT 1 cut(s) 304
AgsI TTSAA 1 cut(s) 109
AluBI AGCT 2 cut(s) 301, 368
AluI AGCT 2 cut(s) 301, 368
Alw21I GWGCWC 1 cut(s) 370
Alw26I GTCTC 1 cut(s) 25
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 2 cut(s) 249, 354
ApeKI GCWGC 4 cut(s) 4, 21, 161, 298
ApoI RAATTY 2 cut(s) 117, 286
ArsI GACNNNNNNTTYG 2 cut(s) 259, 291
AsiGI ACCGGT 1 cut(s) 304
AspS9I GGNCC 3 cut(s) 95, 168, 249
AsuC2I CCSGG 3 cut(s) 28, 113, 144
AsuHPI GGTGA 2 cut(s) 319, 371
AvaII GGWCC 2 cut(s) 95, 168
BanII GRGCYC 1 cut(s) 370
BauI CACGAG 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 370
BbvI GCAGC 3 cut(s) 8, 148, 310
BcnI CCSGG 3 cut(s) 28, 113, 144
BcoDI GTCTC 1 cut(s) 25
BfaI CTAG 1 cut(s) 165
BisI GCNGC 5 cut(s) 5, 22, 162, 299, 354
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 5 cut(s) 6, 23, 163, 300, 355
Bme1390I CCNGG 3 cut(s) 28, 113, 144
Bme18I GGWCC 2 cut(s) 95, 168
BmgT120I GGNCC 3 cut(s) 95, 168, 249
BmrFI CCNGG 3 cut(s) 28, 113, 144
BplI GAGNNNNNCTC 2 cut(s) 22, 54
Bpu1102I GCTNAGC 1 cut(s) 57
BpuMI CCSGG 3 cut(s) 28, 113, 144
BsaI GGTCTC 1 cut(s) 25
BsaWI WCCGGW 7 cut(s) 67, 188, 290, 304, 311, 334, 341
Bsc4I CCNNNNNNNGG 5 cut(s) 311, 318, 341, 356, 370
Bse118I RCCGGY 3 cut(s) 34, 304, 356
Bse1I ACTGG 1 cut(s) 212
BseAI TCCGGA 1 cut(s) 334
BseGI GGATG 1 cut(s) 153
BseLI CCNNNNNNNGG 5 cut(s) 311, 318, 341, 356, 370
BseNI ACTGG 1 cut(s) 212
BseX3I CGGCCG 1 cut(s) 354
BseXI GCAGC 3 cut(s) 8, 148, 310
Bsh1236I CGCG 1 cut(s) 176
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 2 cut(s) 251, 356
BshTI ACCGGT 1 cut(s) 304
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 370
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 5 cut(s) 311, 318, 341, 356, 370
BsmAI GTCTC 1 cut(s) 25
BsmFI GGGAC 1 cut(s) 364
BsnI GGCC 2 cut(s) 251, 356
Bso31I GGTCTC 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 370
Bsp13I TCCGGA 1 cut(s) 334
Bsp143I GATC 2 cut(s) 130, 135
Bsp1720I GCTNAGC 1 cut(s) 57
BspACI CCGC 2 cut(s) 176, 353
BspANI GGCC 2 cut(s) 251, 356
BspEI TCCGGA 1 cut(s) 334
BspFNI CGCG 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 25
BsrFI RCCGGY 3 cut(s) 34, 304, 356
BsrI ACTGG 1 cut(s) 212
BssAI RCCGGY 3 cut(s) 34, 304, 356
BssMI GATC 2 cut(s) 130, 135
BssSI CACGAG 1 cut(s) 221
Bst2BI CACGAG 1 cut(s) 221
BstC8I GCNNGC 3 cut(s) 40, 63, 239
BstDEI CTNAG 2 cut(s) 57, 399
BstEII GGTNACC 2 cut(s) 307, 359
BstF5I GGATG 1 cut(s) 153
BstFNI CGCG 1 cut(s) 176
BstKTI GATC 2 cut(s) 133, 138
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 2 cut(s) 130, 135
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstPI GGTNACC 2 cut(s) 307, 359
BstSCI CCNGG 3 cut(s) 26, 111, 142
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 3 cut(s) 8, 148, 310
BstZI CGGCCG 1 cut(s) 354
BsuRI GGCC 2 cut(s) 251, 356
BtsCI GGATG 1 cut(s) 153
Cac8I GCNNGC 3 cut(s) 40, 63, 239
Cfr10I RCCGGY 3 cut(s) 34, 304, 356
Cfr13I GGNCC 3 cut(s) 95, 168, 249
CseI GACGC 2 cut(s) 62, 182
CspAI ACCGGT 1 cut(s) 304
CviJI RGCY 9 cut(s) 38, 61, 127, 183, 237, 251, 301, 356, 368
CviKI_1 RGCY 9 cut(s) 38, 61, 127, 183, 237, 251, 301, 356, 368
DdeI CTNAG 2 cut(s) 57, 399
DpnI GATC 2 cut(s) 132, 137
DpnII GATC 2 cut(s) 130, 135
EaeI YGGCCR 1 cut(s) 354
EagI CGGCCG 1 cut(s) 354
Ecl136II GAGCTC 1 cut(s) 368
EclXI CGGCCG 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 370
Eco31I GGTCTC 1 cut(s) 25
Eco47I GGWCC 2 cut(s) 95, 168
Eco52I CGGCCG 1 cut(s) 354
Eco53kI GAGCTC 1 cut(s) 368
Eco57I CTGAAG 1 cut(s) 143
Eco91I GGTNACC 2 cut(s) 307, 359
EcoICRI GAGCTC 1 cut(s) 368
EcoO65I GGTNACC 2 cut(s) 307, 359
EcoT38I GRGCYC 1 cut(s) 370
FaiI YATR 1 cut(s) 386
FaqI GGGAC 1 cut(s) 364
Fnu4HI GCNGC 5 cut(s) 5, 22, 162, 299, 354
FokI GGATG 1 cut(s) 140
FriOI GRGCYC 1 cut(s) 370
Fsp4HI GCNGC 5 cut(s) 5, 22, 162, 299, 354
FspBI CTAG 1 cut(s) 165
GluI GCNGC 5 cut(s) 5, 22, 162, 299, 354
HaeIII GGCC 2 cut(s) 251, 356
HgaI GACGC 2 cut(s) 62, 182
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 4 cut(s) 147, 315, 331, 338
HphI GGTGA 2 cut(s) 319, 371
Hpy166II GTNNAC 2 cut(s) 151, 226
Hpy188I TCNGA 3 cut(s) 123, 135, 284
Hpy188III TCNNGA 2 cut(s) 223, 335
Hpy8I GTNNAC 2 cut(s) 151, 226
Hpy99I CGWCG 3 cut(s) 18, 56, 176
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 2 cut(s) 57, 399
Kpn2I TCCGGA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 130, 135
LmnI GCTCC 3 cut(s) 29, 70, 373
Lsp1109I GCAGC 3 cut(s) 8, 148, 310
MaeI CTAG 1 cut(s) 165
MaeIII GTNAC 2 cut(s) 307, 359
MalI GATC 2 cut(s) 132, 137
MboI GATC 2 cut(s) 130, 135
MhlI GDGCHC 1 cut(s) 370
MluCI AATT 2 cut(s) 117, 286
MlyI GAGTC 1 cut(s) 156
MnlI CCTC 4 cut(s) 86, 167, 228, 253
MroI TCCGGA 1 cut(s) 334
MseI TTAA 1 cut(s) 185
MspR9I CCNGG 3 cut(s) 28, 113, 144
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 62
NciI CCSGG 3 cut(s) 28, 113, 144
NdeII GATC 2 cut(s) 130, 135
NmuCI GTSAC 2 cut(s) 307, 359
PfeI GAWTC 3 cut(s) 315, 331, 338
PfoI TCCNGGA 2 cut(s) 26, 142
PinAI ACCGGT 1 cut(s) 304
PkrI GCNGC 5 cut(s) 6, 23, 163, 300, 355
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
Psp124BI GAGCTC 1 cut(s) 370
PspEI GGTNACC 2 cut(s) 307, 359
PspPI GGNCC 3 cut(s) 95, 168, 249
SacI GAGCTC 1 cut(s) 370
SaqAI TTAA 1 cut(s) 185
SatI GCNGC 5 cut(s) 5, 22, 162, 299, 354
Sau3AI GATC 2 cut(s) 130, 135
Sau96I GGNCC 3 cut(s) 95, 168, 249
SchI GAGTC 1 cut(s) 156
ScrFI CCNGG 3 cut(s) 28, 113, 144
SduI GDGCHC 1 cut(s) 370
SetI ASST 3 cut(s) 272, 303, 370
SinI GGWCC 2 cut(s) 95, 168
Sse9I AATT 2 cut(s) 117, 286
SsiI CCGC 2 cut(s) 176, 353
SspMI CTAG 1 cut(s) 165
SstI GAGCTC 1 cut(s) 370
StyD4I CCNGG 3 cut(s) 26, 111, 142
TaqII GACCGA 3 cut(s) 112, 185, 378
TasI AATT 2 cut(s) 117, 286
TauI GCSGC 1 cut(s) 356
TfiI GAWTC 3 cut(s) 315, 331, 338
Tru1I TTAA 1 cut(s) 185
Tru9I TTAA 1 cut(s) 185
TseFI GTSAC 2 cut(s) 307, 359
TseI GCWGC 4 cut(s) 4, 21, 161, 298
Tsp45I GTSAC 2 cut(s) 307, 359
TspGWI ACGGA 1 cut(s) 215
VpaK11BI GGWCC 2 cut(s) 95, 168
XapI RAATTY 2 cut(s) 117, 286
XspI CTAG 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.