Rmu_sc0000388.1_g000047

Component of the DNA topoisomerase VI involved in chromatin organization and progression of endoreduplication cycles. Relaxes both positive and negative superturns and exhibits a strong decatenase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000388.1
OG0004536
Physical Location & Seq
Forward (+)
246837 .. 247445
609 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 609 bp
atgtcatccatcgccttctctttcaatcaagaatgttccaacaaatttcaagttgaagacctctctctcgaggagatagcaataaggattcaagattataaaaaggatatatcagaaggtaaggaactaaatttgatttcaagaggtcgttccaatgtgattttttccagcaatcgtattttactaaaggatcaaggtattcgttataaaccaagggatcgtaagaaagacatcatccttcgtcctcttgacattattctcatgtttttcaatggacataagcgctacacactttccagcctatattatatggatacgaagctcttcgaacagcaagctgtatctgacagtgctttggatgacattttttgcatcattggttgcagccgctcgagtctcaacgtgtttgcctctgacagaggttatgtgggaggtgtcctcacttttaggctagatgatcaccaatttgattgtcgtaggatgggaccatatggccaaccaatccttccttttattgatgatcagcttaatgatgtacagactcatggtgcactgttcatattatatgtggagaagcataataaggaaaaactccataatgattattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04113 map04113
Pfam Domains
Protein Families
TP6A_N

Protein Analysis

202

Amino Acids

23.48

Weight (kDa)

6.52

Isoelectric Point (pI)

47.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP6A_N PF04406 78 - 135 2.9e-11 Type IIB DNA topoisomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 99, 207
AccBSI CCGCTC 1 cut(s) 390
AciI CCGC 1 cut(s) 388
AclWI GGATC 2 cut(s) 198, 225
AcoI YGGCCR 1 cut(s) 493
AcsI RAATTY 2 cut(s) 44, 130
AfaI GTAC 1 cut(s) 537
AfeI AGCGCT 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 6 cut(s) 25, 50, 56, 92, 141, 271
AjuI GAANNNNNNNTTGG 2 cut(s) 489, 521
AluBI AGCT 3 cut(s) 322, 338, 526
AluI AGCT 3 cut(s) 322, 338, 526
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 1 cut(s) 401
Alw44I GTGCAC 1 cut(s) 549
AlwI GGATC 2 cut(s) 198, 225
Ama87I CYCGRG 2 cut(s) 68, 391
Aor51HI AGCGCT 1 cut(s) 284
AoxI GGCC 1 cut(s) 493
ApaLI GTGCAC 1 cut(s) 549
ApeKI GCWGC 1 cut(s) 384
ApoI RAATTY 2 cut(s) 44, 130
Asp700I GAANNNNTTC 1 cut(s) 323
AspLEI GCGC 1 cut(s) 285
AspS9I GGNCC 1 cut(s) 485
AsuHPI GGTGA 1 cut(s) 452
AsuII TTCGAA 1 cut(s) 327
AvaI CYCGRG 2 cut(s) 68, 391
AvaII GGWCC 1 cut(s) 485
BaeGI GKGCMC 1 cut(s) 553
BalI TGGCCA 1 cut(s) 495
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 1 cut(s) 396
BccI CCATC 2 cut(s) 17, 475
BciVI GTATCC 1 cut(s) 307
BclI TGATCA 2 cut(s) 457, 520
BcoDI GTCTC 1 cut(s) 401
BfaI CTAG 1 cut(s) 452
BfoI RGCGCY 1 cut(s) 286
BfuI GTATCC 1 cut(s) 307
BisI GCNGC 2 cut(s) 385, 388
BlsI GCNGC 2 cut(s) 386, 389
Bme18I GGWCC 1 cut(s) 485
BmeT110I CYCGRG 2 cut(s) 68, 391
BmgT120I GGNCC 1 cut(s) 485
BmiI GGNNCC 1 cut(s) 486
BmsI GCATC 1 cut(s) 381
BpiI GAAGAC 1 cut(s) 63
BplI GAGNNNNNCTC 2 cut(s) 423, 455
Bpu14I TTCGAA 1 cut(s) 327
BsaJI CCNNGG 1 cut(s) 212
BseDI CCNNGG 1 cut(s) 212
BseGI GGATG 4 cut(s) 5, 234, 364, 486
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 1 cut(s) 396
BshFI GGCC 1 cut(s) 495
BsiHKAI GWGCWC 1 cut(s) 553
BsiHKCI CYCGRG 2 cut(s) 68, 391
BslFI GGGAC 1 cut(s) 498
BsmAI GTCTC 1 cut(s) 401
BsmFI GGGAC 1 cut(s) 498
BsnI GGCC 1 cut(s) 495
BsoBI CYCGRG 2 cut(s) 68, 391
Bsp119I TTCGAA 1 cut(s) 327
Bsp1286I GDGCHC 1 cut(s) 553
Bsp1407I TGTACA 1 cut(s) 535
Bsp143I GATC 4 cut(s) 190, 217, 457, 520
BspACI CCGC 1 cut(s) 388
BspANI GGCC 1 cut(s) 495
BspLI GGNNCC 1 cut(s) 486
BspPI GGATC 2 cut(s) 198, 225
BspQI GCTCTTC 1 cut(s) 329
BspT104I TTCGAA 1 cut(s) 327
BsrBI CCGCTC 1 cut(s) 390
BsrGI TGTACA 1 cut(s) 535
BssECI CCNNGG 1 cut(s) 212
BssMI GATC 4 cut(s) 190, 217, 457, 520
BssT1I CCWWGG 1 cut(s) 212
Bst4CI ACNGT 2 cut(s) 350, 555
Bst6I CTCTTC 1 cut(s) 329
BstAUI TGTACA 1 cut(s) 535
BstBI TTCGAA 1 cut(s) 327
BstC8I GCNNGC 1 cut(s) 336
BstF5I GGATG 4 cut(s) 5, 234, 364, 486
BstH2I RGCGCY 1 cut(s) 286
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 4 cut(s) 193, 220, 460, 523
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 4 cut(s) 190, 217, 457, 520
BstSLI GKGCMC 1 cut(s) 553
BstV1I GCAGC 1 cut(s) 396
BstV2I GAAGAC 1 cut(s) 63
BsuI GTATCC 1 cut(s) 307
BsuRI GGCC 1 cut(s) 495
BtsCI GGATG 4 cut(s) 5, 234, 364, 486
BtsIMutI CAGTG 2 cut(s) 355, 551
Cac8I GCNNGC 1 cut(s) 336
CfoI GCGC 1 cut(s) 285
Cfr13I GGNCC 1 cut(s) 485
Csp6I GTAC 1 cut(s) 536
CviAII CATG 2 cut(s) 262, 545
CviJI RGCY 7 cut(s) 300, 322, 338, 387, 451, 495, 526
CviKI_1 RGCY 7 cut(s) 300, 322, 338, 387, 451, 495, 526
CviQI GTAC 1 cut(s) 536
DpnI GATC 4 cut(s) 192, 219, 459, 522
DpnII GATC 4 cut(s) 190, 217, 457, 520
EaeI YGGCCR 1 cut(s) 493
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
Eco130I CCWWGG 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 485
Eco47III AGCGCT 1 cut(s) 284
Eco88I CYCGRG 2 cut(s) 68, 391
EcoT14I CCWWGG 1 cut(s) 212
ErhI CCWWGG 1 cut(s) 212
FaeI CATG 2 cut(s) 265, 548
FaqI GGGAC 1 cut(s) 498
FatI CATG 2 cut(s) 261, 544
FauNDI CATATG 1 cut(s) 490
FbaI TGATCA 2 cut(s) 457, 520
Fnu4HI GCNGC 2 cut(s) 385, 388
FokI GGATG 3 cut(s) 221, 371, 493
Fsp4HI GCNGC 2 cut(s) 385, 388
FspBI CTAG 1 cut(s) 452
GlaI GCGC 1 cut(s) 284
GluI GCNGC 2 cut(s) 385, 388
HaeII RGCGCY 1 cut(s) 286
HaeIII GGCC 1 cut(s) 495
HhaI GCGC 1 cut(s) 285
Hin1II CATG 2 cut(s) 265, 548
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HinfI GANTC 3 cut(s) 88, 394, 541
HphI GGTGA 1 cut(s) 452
Hpy166II GTNNAC 1 cut(s) 551
Hpy188I TCNGA 3 cut(s) 115, 346, 415
Hpy188III TCNNGA 5 cut(s) 29, 68, 92, 141, 248
Hpy8I GTNNAC 1 cut(s) 551
HpyAV CCTTC 4 cut(s) 25, 110, 248, 515
HpyCH4III ACNGT 2 cut(s) 350, 555
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 3 cut(s) 372, 384, 551
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 2 cut(s) 265, 548
HspAI GCGC 1 cut(s) 283
Ksp22I TGATCA 2 cut(s) 457, 520
Kzo9I GATC 4 cut(s) 190, 217, 457, 520
LguI GCTCTTC 1 cut(s) 329
LpnPI CCDG 2 cut(s) 181, 310
Lsp1109I GCAGC 1 cut(s) 396
LweI GCATC 1 cut(s) 381
MaeI CTAG 1 cut(s) 452
MaeII ACGT 1 cut(s) 402
MalI GATC 4 cut(s) 192, 219, 459, 522
MbiI CCGCTC 1 cut(s) 390
MboI GATC 4 cut(s) 190, 217, 457, 520
MboII GAAGA 2 cut(s) 68, 316
MhlI GDGCHC 1 cut(s) 553
MlsI TGGCCA 1 cut(s) 495
MluCI AATT 3 cut(s) 44, 130, 464
MluNI TGGCCA 1 cut(s) 495
MlyI GAGTC 2 cut(s) 403, 535
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 8 cut(s) 64, 71, 137, 255, 413, 421, 425, 449
Mox20I TGGCCA 1 cut(s) 495
MroXI GAANNNNTTC 1 cut(s) 323
MscI TGGCCA 1 cut(s) 495
MseI TTAA 1 cut(s) 528
Msp20I TGGCCA 1 cut(s) 495
NdeI CATATG 1 cut(s) 490
NdeII GATC 4 cut(s) 190, 217, 457, 520
NlaIII CATG 2 cut(s) 265, 548
NlaIV GGNNCC 1 cut(s) 486
NspV TTCGAA 1 cut(s) 327
PaeR7I CTCGAG 2 cut(s) 68, 391
PciSI GCTCTTC 1 cut(s) 329
PdmI GAANNNNTTC 1 cut(s) 323
PfeI GAWTC 1 cut(s) 88
PkrI GCNGC 2 cut(s) 386, 389
PleI GAGTC 2 cut(s) 402, 535
PpsI GAGTC 2 cut(s) 402, 535
PsiI TTATAA 2 cut(s) 99, 207
PspN4I GGNNCC 1 cut(s) 486
PspPI GGNCC 1 cut(s) 485
PspXI VCTCGAGB 1 cut(s) 391
RsaI GTAC 1 cut(s) 537
RsaNI GTAC 1 cut(s) 536
SapI GCTCTTC 1 cut(s) 329
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 2 cut(s) 385, 388
Sau3AI GATC 4 cut(s) 190, 217, 457, 520
Sau96I GGNCC 1 cut(s) 485
SchI GAGTC 2 cut(s) 403, 535
SduI GDGCHC 1 cut(s) 553
SfaNI GCATC 1 cut(s) 381
Sfr274I CTCGAG 2 cut(s) 68, 391
SfuI TTCGAA 1 cut(s) 327
SinI GGWCC 1 cut(s) 485
SlaI CTCGAG 2 cut(s) 68, 391
SmlI CTYRAG 2 cut(s) 68, 391
SmoI CTYRAG 2 cut(s) 68, 391
Sse9I AATT 3 cut(s) 44, 130, 464
SsiI CCGC 1 cut(s) 388
SspMI CTAG 1 cut(s) 452
StyI CCWWGG 1 cut(s) 212
TaaI ACNGT 2 cut(s) 350, 555
TaiI ACGT 1 cut(s) 405
TaqI TCGA 3 cut(s) 69, 327, 392
TasI AATT 3 cut(s) 44, 130, 464
TatI WGTACW 1 cut(s) 535
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 1 cut(s) 88
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TscAI CASTG 2 cut(s) 355, 558
TseI GCWGC 1 cut(s) 384
TspDTI ATGAA 1 cut(s) 547
TspRI CASTG 2 cut(s) 355, 558
VneI GTGCAC 1 cut(s) 549
VpaK11BI GGWCC 1 cut(s) 485
XapI RAATTY 2 cut(s) 44, 130
XhoI CTCGAG 2 cut(s) 68, 391
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.