Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000418.1

Orthogroup ID

OG0003830

Physical Location & Seq

Genomic Coordinates Forward (+)

54005 .. 58398

Gene Structure 4394 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1242 bp

atgctagacttccagggcttcaaaataggagtcgaattaattagggttagggatttgtgtcgattttcaagaggcttcatgattttggtcgcgattcaggttgagatgacttttcgtattggattcgatcattggagatatggatgtgggttccatctgaccgagttctctcacatggccgacccttcctttgagcttggctgccctggcgaggagagcagggcagttgatacagagtcgctttgtattctttctagcaagttggtgtgggctatacgtgttgacctccgcattctagataatggagggaatcttattgatgctactaatattgttgcactggctggtttgttgacgttctggaggcctgagtgctcattaggagaagaaaatggtcagggaatcatcatatgtccacccgaggatgctattttgaatgaagatgaaggcctgaaactaaaaaggagaagaattccaccagctctcaacctattcacaaagacaattgataagaacctcgcaacaagcccgttcaagatgcttctgaagtacaggcctaaagacaaagcagagaagaaggagcgtctattgaaaagggctcaggcagagactgaaggaaagactaacaaggcccaacttgtggttaccgctcatgatgtcaatccaattgagttggttgtatggctccctgctttatgcagaaagatggagatcccctattgcattgtgaagggcaaagcacgtttgctgcctctttattatcggctgcccatgccgcttcttcctctcaatctttccgattccctaaagtccaaagctttcctttctctcctcctcctccccaaacccaaatcttctgttcctcgctacaagccttacccactctccctcagctctccacatgaacgctcccacatcggaacagaagccctcctttcaggtctcactgcaattctcacacccttctttgttttctattcattctttgatggctcaccttgtcgacctcgtcattctctaaaactttcgaaccctacatccttcttcagcagctcacctcgtcgacctcaccttgtcaacctcatcattctccaagccgcctccgacatgaccccgagtccccgacgtcagtctctgaccccatgttcccctgcctcgctctttgaccccgagtccccgatctcgttctccagacctctagacctcgagcttgatctatcatcatctgcactcccagtctga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000470 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0006139 GO:0006364 GO:0006396 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0015934 GO:0016070 GO:0016072 GO:0019538 GO:0022613 GO:0022625 GO:0022626 GO:0032991 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0042254 GO:0042273 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03010 map03010

Pfam Domains

Protein Families

Ribosomal_L7Ae

Protein Analysis

413

Amino Acids

46.33

Weight (kDa)

9.09

Isoelectric Point (pI)

53.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribosomal_L7Ae	PF01248	207 - 249	1.2e-10	Ribosomal protein L7Ae/L30e/S12e/Gadd45 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003830)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12990 AT3G12990 AT3G12990 AT3G12990 AT3G60500 AT3G60500 AT3G60500 AT3G60500
fragaria_vesca	FvH4_1g30010 FvH4_1g30010
malus_domestica	MD03G1172100.v1.1 MD11G1189800.v1.1
prunus_persica	Prupe.4G254800_v2.0.a1 Prupe.4G254800_v2.0.a1 Prupe.4G254800_v2.0.a1
pyrus_communis	pycom03g12920
rosa_chinensis	RchiOBHm_Chr2g0134251 RchiOBHm_Chr3g0492251
rosa_laevigata	RLG00000022789
rosa_multiflora	Rmu_sc0000418.1_g000008 Rmu_sc0002530.1_g000013 Rmu_sc0003579.1_g000006
rosa_roxburghii	Rroxscaffold_6G00392470
rosa_rugosa	Rorug02G0315300 Rorug03G0252700
rosa_samantha	Rh2AG367900 Rh2BG374300 Rh2CG351900 Rh2DG391500 Rh3AG302700 Rh3BG338700 Rh3CG335800 Rh3DG337900
rosa_wichuraiana	Rw3G026700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1129
AccB7I	CCANNNNNTGG	1 cut(s)	640
AccBSI	CCGCTC	1 cut(s)	650
AccI	GTMKAC	2 cut(s)	1003, 1063
AccII	CGCG	1 cut(s)	92
AciI	CCGC	4 cut(s)	289, 648, 776, 1098
AclWI	GGATC	1 cut(s)	706
AcoI	YGGCCR	1 cut(s)	177
AcsI	RAATTY	1 cut(s)	471
AcuI	CTGAAG	3 cut(s)	566, 633, 1030
AcyI	GRCGYC	1 cut(s)	1126
AfaI	GTAC	1 cut(s)	551
AfiI	CCNNNNNNNGG	2 cut(s)	211, 640
AflIII	ACRYGT	1 cut(s)	277
AgsI	TTSAA	5 cut(s)	22, 69, 436, 535, 592
AhdI	GACNNNNNGTC	2 cut(s)	1116, 1171
AjnI	CCWGG	2 cut(s)	12, 205
AloI	GAACNNNNNNTCC	2 cut(s)	1022, 1054
AluBI	AGCT	6 cut(s)	196, 482, 818, 894, 1053, 1210
AluI	AGCT	6 cut(s)	196, 482, 818, 894, 1053, 1210
Alw21I	GWGCWC	1 cut(s)	377
Alw26I	GTCTC	3 cut(s)	602, 947, 1137
AlwI	GGATC	1 cut(s)	706
AlwNI	CAGNNNCTG	2 cut(s)	611, 1135
Ama87I	CYCGRG	4 cut(s)	419, 1114, 1169, 1205
AoxI	GGCC	5 cut(s)	177, 365, 448, 554, 630
ApeKI	GCWGC	4 cut(s)	201, 748, 766, 1050
ApoI	RAATTY	1 cut(s)	471
ArsI	GACNNNNNNTTYG	4 cut(s)	15, 47, 173, 205
AseI	ATTAAT	1 cut(s)	38
AspS9I	GGNCC	1 cut(s)	631
AsuHPI	GGTGA	3 cut(s)	987, 1047, 1061
AsuII	TTCGAA	1 cut(s)	1028
AvaI	CYCGRG	4 cut(s)	419, 1114, 1169, 1205
BanII	GRGCYC	1 cut(s)	601
Bbv12I	GWGCWC	1 cut(s)	377
BbvCI	CCTCAGC	1 cut(s)	890
BbvI	GCAGC	4 cut(s)	188, 735, 753, 1062
BccI	CCATC	3 cut(s)	162, 700, 983
BciT130I	CCWGG	2 cut(s)	14, 207
BcoDI	GTCTC	3 cut(s)	602, 947, 1137
BfaI	CTAG	4 cut(s)	5, 255, 296, 1199
BisI	GCNGC	6 cut(s)	202, 749, 767, 776, 1051, 1098
BlsI	GCNGC	6 cut(s)	203, 750, 768, 777, 1052, 1099
Bme1390I	CCNGG	2 cut(s)	14, 207
BmeRI	GACNNNNNGTC	2 cut(s)	1116, 1171
BmeT110I	CYCGRG	4 cut(s)	419, 1114, 1169, 1205
BmgT120I	GGNCC	1 cut(s)	631
BmiI	GGNNCC	2 cut(s)	152, 686
BmrFI	CCNGG	2 cut(s)	14, 207
BmrI	ACTGGG	1 cut(s)	1229
BmsI	GCATC	3 cut(s)	310, 415, 528
BmuI	ACTGGG	1 cut(s)	1229
BoxI	GACNNNNGTC	1 cut(s)	1129
BpmI	CTGGAG	2 cut(s)	382, 1174
Bpu10I	CCTNAGC	2 cut(s)	600, 890
Bpu14I	TTCGAA	1 cut(s)	1028
BsaAI	YACGTR	1 cut(s)	278
BsaBI	GATNNNNATC	2 cut(s)	660, 710
BsaHI	GRCGYC	1 cut(s)	1126
BsaI	GGTCTC	1 cut(s)	947
BsaJI	CCNNGG	3 cut(s)	13, 205, 420
BsaXI	ACNNNNNCTCC	2 cut(s)	869, 899
Bsc4I	CCNNNNNNNGG	2 cut(s)	211, 640
Bse1I	ACTGG	2 cut(s)	345, 1235
Bse8I	GATNNNNATC	2 cut(s)	660, 710
BseBI	CCWGG	2 cut(s)	14, 207
BseDI	CCNNGG	3 cut(s)	13, 205, 420
BseGI	GGATG	3 cut(s)	149, 430, 1037
BseJI	GATNNNNATC	2 cut(s)	660, 710
BseLI	CCNNNNNNNGG	2 cut(s)	211, 640
BseMII	CTCAG	3 cut(s)	360, 614, 904
BseNI	ACTGG	2 cut(s)	345, 1235
BseRI	GAGGAG	4 cut(s)	227, 821, 824, 827
BseXI	GCAGC	4 cut(s)	188, 735, 753, 1062
BsgI	GTGCAG	1 cut(s)	1212
Bsh1236I	CGCG	1 cut(s)	92
BshFI	GGCC	5 cut(s)	179, 367, 450, 556, 632
BsiHKAI	GWGCWC	1 cut(s)	377
BsiHKCI	CYCGRG	4 cut(s)	419, 1114, 1169, 1205
BslFI	GGGAC	2 cut(s)	1104, 1159
BslI	CCNNNNNNNGG	2 cut(s)	211, 640
BsmAI	GTCTC	3 cut(s)	602, 947, 1137
BsmFI	GGGAC	2 cut(s)	1104, 1159
BsmI	GAATGC	1 cut(s)	291
BsnI	GGCC	5 cut(s)	179, 367, 450, 556, 632
Bso31I	GGTCTC	1 cut(s)	947
BsoBI	CYCGRG	4 cut(s)	419, 1114, 1169, 1205
Bsp119I	TTCGAA	1 cut(s)	1028
Bsp1286I	GDGCHC	2 cut(s)	377, 601
Bsp143I	GATC	4 cut(s)	127, 711, 1179, 1213
Bsp68I	TCGCGA	1 cut(s)	92
BspACI	CCGC	4 cut(s)	289, 648, 776, 1098
BspANI	GGCC	5 cut(s)	179, 367, 450, 556, 632
BspCNI	CTCAG	3 cut(s)	361, 613, 903
BspFNI	CGCG	1 cut(s)	92
BspHI	TCATGA	2 cut(s)	78, 652
BspLI	GGNNCC	2 cut(s)	152, 686
BspPI	GGATC	1 cut(s)	706
BspT104I	TTCGAA	1 cut(s)	1028
BspTNI	GGTCTC	1 cut(s)	947
BsrBI	CCGCTC	1 cut(s)	650
BsrI	ACTGG	2 cut(s)	345, 1235
BssECI	CCNNGG	3 cut(s)	13, 205, 420
BssMI	GATC	4 cut(s)	127, 711, 1179, 1213
BssNI	GRCGYC	1 cut(s)	1126
Bst2UI	CCWGG	2 cut(s)	14, 207
BstACI	GRCGYC	1 cut(s)	1126
BstBAI	YACGTR	1 cut(s)	278
BstBI	TTCGAA	1 cut(s)	1028
BstDEI	CTNAG	3 cut(s)	369, 600, 890
BstEII	GGTNACC	1 cut(s)	643
BstF5I	GGATG	3 cut(s)	149, 430, 1037
BstFNI	CGCG	1 cut(s)	92
BstKTI	GATC	4 cut(s)	130, 714, 1182, 1216
BstMAI	GTCTC	3 cut(s)	602, 947, 1137
BstMBI	GATC	4 cut(s)	127, 711, 1179, 1213
BstMWI	GCNNNNNNNGC	4 cut(s)	207, 216, 772, 775
BstNI	CCWGG	2 cut(s)	14, 207
BstPAI	GACNNNNGTC	1 cut(s)	1129
BstPI	GGTNACC	1 cut(s)	643
BstSCI	CCNGG	2 cut(s)	12, 205
BstUI	CGCG	1 cut(s)	92
BstV1I	GCAGC	4 cut(s)	188, 735, 753, 1062
BstX2I	RGATCY	1 cut(s)	711
BstYI	RGATCY	1 cut(s)	711
BsuRI	GGCC	5 cut(s)	179, 367, 450, 556, 632
BtsCI	GGATG	3 cut(s)	149, 430, 1037
BtsI	GCAGTG	1 cut(s)	945
BtsIMutI	CAGTG	2 cut(s)	338, 945
BtuMI	TCGCGA	1 cut(s)	92
CaiI	CAGNNNCTG	2 cut(s)	611, 1135
CciI	TCATGA	2 cut(s)	78, 652
Cfr13I	GGNCC	1 cut(s)	631
CseI	GACGC	1 cut(s)	572
Csp6I	GTAC	1 cut(s)	550
CviAII	CATG	7 cut(s)	79, 175, 653, 772, 902, 1108, 1143
CviQI	GTAC	1 cut(s)	550
DdeI	CTNAG	3 cut(s)	369, 600, 890
DpnI	GATC	4 cut(s)	129, 713, 1181, 1215
DpnII	GATC	4 cut(s)	127, 711, 1179, 1213
DriI	GACNNNNNGTC	2 cut(s)	1116, 1171
EaeI	YGGCCR	1 cut(s)	177
Eam1105I	GACNNNNNGTC	2 cut(s)	1116, 1171
Eco147I	AGGCCT	3 cut(s)	367, 450, 556
Eco24I	GRGCYC	1 cut(s)	601
Eco31I	GGTCTC	1 cut(s)	947
Eco57I	CTGAAG	3 cut(s)	566, 633, 1030
Eco88I	CYCGRG	4 cut(s)	419, 1114, 1169, 1205
Eco91I	GGTNACC	1 cut(s)	643
EcoO65I	GGTNACC	1 cut(s)	643
EcoRI	GAATTC	1 cut(s)	471
EcoRII	CCWGG	2 cut(s)	12, 205
EcoT38I	GRGCYC	1 cut(s)	601
FaeI	CATG	7 cut(s)	82, 178, 656, 775, 905, 1111, 1146
FalI	AAGNNNNNCTT	4 cut(s)	807, 839, 918, 950
FaqI	GGGAC	2 cut(s)	1104, 1159
FatI	CATG	7 cut(s)	78, 174, 652, 771, 901, 1107, 1142
FauNDI	CATATG	1 cut(s)	410
FblI	GTMKAC	2 cut(s)	1003, 1063
Fnu4HI	GCNGC	6 cut(s)	202, 749, 767, 776, 1051, 1098
FokI	GGATG	3 cut(s)	156, 437, 1024
FriOI	GRGCYC	1 cut(s)	601
Fsp4HI	GCNGC	6 cut(s)	202, 749, 767, 776, 1051, 1098
FspBI	CTAG	4 cut(s)	5, 255, 296, 1199
GluI	GCNGC	6 cut(s)	202, 749, 767, 776, 1051, 1098
GsuI	CTGGAG	2 cut(s)	382, 1174
HaeIII	GGCC	5 cut(s)	179, 367, 450, 556, 632
HgaI	GACGC	1 cut(s)	572
Hin1I	GRCGYC	1 cut(s)	1126
Hin1II	CATG	7 cut(s)	82, 178, 656, 775, 905, 1111, 1146
HincII	GTYRAC	5 cut(s)	283, 354, 1004, 1064, 1078
HindII	GTYRAC	5 cut(s)	283, 354, 1004, 1064, 1078
HindIII	AAGCTT	1 cut(s)	816
HinfI	GANTC	9 cut(s)	30, 94, 123, 236, 310, 402, 800, 1117, 1172
HphI	GGTGA	3 cut(s)	987, 1047, 1061
Hpy166II	GTNNAC	6 cut(s)	283, 354, 416, 1004, 1064, 1078
Hpy188I	TCNGA	7 cut(s)	159, 546, 799, 920, 1105, 1137, 1241
Hpy188III	TCNNGA	9 cut(s)	69, 79, 91, 296, 361, 535, 653, 1191, 1199
Hpy8I	GTNNAC	6 cut(s)	283, 354, 416, 1004, 1064, 1078
Hpy99I	CGWCG	2 cut(s)	1065, 1128
HpyAV	CCTTC	7 cut(s)	195, 440, 571, 608, 724, 973, 1051
HpyCH4IV	ACGT	4 cut(s)	277, 356, 742, 1126
HpyCH4V	TGCA	5 cut(s)	338, 699, 723, 950, 1229
HpyF10VI	GCNNNNNNNGC	4 cut(s)	207, 216, 772, 775
HpyF3I	CTNAG	3 cut(s)	369, 600, 890
HpySE526I	ACGT	4 cut(s)	277, 356, 742, 1126
Hsp92I	GRCGYC	1 cut(s)	1126
Hsp92II	CATG	7 cut(s)	82, 178, 656, 775, 905, 1111, 1146
Kzo9I	GATC	4 cut(s)	127, 711, 1179, 1213
LmnI	GCTCC	3 cut(s)	580, 690, 914
Lsp1109I	GCAGC	4 cut(s)	188, 735, 753, 1062
LweI	GCATC	3 cut(s)	310, 415, 528
MaeI	CTAG	4 cut(s)	5, 255, 296, 1199
MaeII	ACGT	4 cut(s)	277, 356, 742, 1126
MaeIII	GTNAC	1 cut(s)	643
MalI	GATC	4 cut(s)	129, 713, 1181, 1215
MbiI	CCGCTC	1 cut(s)	650
MboI	GATC	4 cut(s)	127, 711, 1179, 1213
MboII	GAAGA	7 cut(s)	398, 452, 480, 586, 773, 846, 1036
MfeI	CAATTG	2 cut(s)	504, 666
MflI	RGATCY	1 cut(s)	711
MhlI	GDGCHC	2 cut(s)	377, 601
MluCI	AATT	6 cut(s)	35, 39, 471, 504, 666, 951
MlyI	GAGTC	4 cut(s)	39, 245, 1126, 1181
MmeI	TCCRAC	1 cut(s)	1128
MseI	TTAA	1 cut(s)	38
MspR9I	CCNGG	2 cut(s)	14, 207
MunI	CAATTG	2 cut(s)	504, 666
Mva1269I	GAATGC	1 cut(s)	291
MvaI	CCWGG	2 cut(s)	14, 207
MvnI	CGCG	1 cut(s)	92
MwoI	GCNNNNNNNGC	4 cut(s)	207, 216, 772, 775
NdeI	CATATG	1 cut(s)	410
NdeII	GATC	4 cut(s)	127, 711, 1179, 1213
NlaIII	CATG	7 cut(s)	82, 178, 656, 775, 905, 1111, 1146
NlaIV	GGNNCC	2 cut(s)	152, 686
NruI	TCGCGA	1 cut(s)	92
NspV	TTCGAA	1 cut(s)	1028
PaeR7I	CTCGAG	1 cut(s)	1205
PagI	TCATGA	2 cut(s)	78, 652
PceI	AGGCCT	3 cut(s)	367, 450, 556
PctI	GAATGC	1 cut(s)	291
PfeI	GAWTC	5 cut(s)	94, 123, 310, 402, 800
PflFI	GACNNNGTC	1 cut(s)	1008
PflMI	CCANNNNNTGG	1 cut(s)	640
PkrI	GCNGC	6 cut(s)	203, 750, 768, 777, 1052, 1099
PleI	GAGTC	4 cut(s)	38, 244, 1125, 1180
PpsI	GAGTC	4 cut(s)	38, 244, 1125, 1180
Ppu21I	YACGTR	1 cut(s)	278
PshAI	GACNNNNGTC	1 cut(s)	1129
PshBI	ATTAAT	1 cut(s)	38
Psp6I	CCWGG	2 cut(s)	12, 205
PspEI	GGTNACC	1 cut(s)	643
PspGI	CCWGG	2 cut(s)	12, 205
PspN4I	GGNNCC	2 cut(s)	152, 686
PspPI	GGNCC	1 cut(s)	631
PspXI	VCTCGAGB	1 cut(s)	1205
PstNI	CAGNNNCTG	2 cut(s)	611, 1135
PsuI	RGATCY	1 cut(s)	711
PsyI	GACNNNGTC	1 cut(s)	1008
RruI	TCGCGA	1 cut(s)	92
RsaI	GTAC	1 cut(s)	551
RsaNI	GTAC	1 cut(s)	550
SalI	GTCGAC	2 cut(s)	1002, 1062
SaqAI	TTAA	1 cut(s)	38
SatI	GCNGC	6 cut(s)	202, 749, 767, 776, 1051, 1098
Sau3AI	GATC	4 cut(s)	127, 711, 1179, 1213
Sau96I	GGNCC	1 cut(s)	631
SchI	GAGTC	4 cut(s)	39, 245, 1126, 1181
ScrFI	CCNGG	2 cut(s)	14, 207
SduI	GDGCHC	2 cut(s)	377, 601
SfaNI	GCATC	3 cut(s)	310, 415, 528
Sfr274I	CTCGAG	1 cut(s)	1205
SfuI	TTCGAA	1 cut(s)	1028
SlaI	CTCGAG	1 cut(s)	1205
SmlI	CTYRAG	1 cut(s)	1205
SmoI	CTYRAG	1 cut(s)	1205
Sse9I	AATT	6 cut(s)	35, 39, 471, 504, 666, 951
SseBI	AGGCCT	3 cut(s)	367, 450, 556
SsiI	CCGC	4 cut(s)	289, 648, 776, 1098
SspI	AATATT	1 cut(s)	331
SspMI	CTAG	4 cut(s)	5, 255, 296, 1199
StuI	AGGCCT	3 cut(s)	367, 450, 556
StyD4I	CCNGG	2 cut(s)	12, 205
TaiI	ACGT	4 cut(s)	280, 359, 745, 1129
TaqI	TCGA	7 cut(s)	33, 61, 126, 1003, 1028, 1063, 1206
TaqII	GACCGA	1 cut(s)	176
TasI	AATT	6 cut(s)	35, 39, 471, 504, 666, 951
TatI	WGTACW	1 cut(s)	549
TauI	GCSGC	2 cut(s)	778, 1100
TfiI	GAWTC	5 cut(s)	94, 123, 310, 402, 800
Tru1I	TTAA	1 cut(s)	38
Tru9I	TTAA	1 cut(s)	38
TscAI	CASTG	2 cut(s)	345, 952
TseI	GCWGC	4 cut(s)	201, 748, 766, 1050
TspDTI	ATGAA	5 cut(s)	67, 453, 459, 918, 969
TspRI	CASTG	2 cut(s)	345, 952
Tth111I	GACNNNGTC	1 cut(s)	1008
Van91I	CCANNNNNTGG	1 cut(s)	640
VspI	ATTAAT	1 cut(s)	38
XapI	RAATTY	1 cut(s)	471
XbaI	TCTAGA	2 cut(s)	295, 1198
XhoI	CTCGAG	1 cut(s)	1205
XmiI	GTMKAC	2 cut(s)	1003, 1063
XspI	CTAG	4 cut(s)	5, 255, 296, 1199
ZraI	GACGTC	1 cut(s)	1127

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.