Rmu_sc0000545.1_g000012

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000545.1
OG0014871
Physical Location & Seq
Reverse (-)
52787 .. 53233
447 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 447 bp
atggactgtggtggctccaaagtagacgacatcaaaacggcgtcggatacccgctacgccttggacgcagcccatctaacttatttccagtcgctcaagcaaatcggcgacgcgctttgcaagttcgttgacgaggacctagtcatcggcggcgtcggcgagtcatattcgctaccggattctccagttctgacattgccctccgtcgaaggaagccctggaaaggaaagccccaagggaaaagctcctcgtcgtcttcgatttcactctcgcatagtcaccggaatggtgagtgtgagttgctcgatggggatgttcctcgatcgccggaaagagatgagagagagagagagagagagaaacagagacgtggagggggaggggagagagagacagagacgtggagggggaggggagagagagagagatcggtctgcagagagatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

148

Amino Acids

16.52

Weight (kDa)

8.49

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 11 - 46 1e-09 Protein of unknown function (DUF630)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 24
AccII CGCG 1 cut(s) 113
AciI CCGC 2 cut(s) 52, 150
AcyI GRCGYC 2 cut(s) 41, 153
AfiI CCNNNNNNNGG 1 cut(s) 223
AjiI CACGTC 2 cut(s) 370, 401
AjnI CCWGG 1 cut(s) 217
AluBI AGCT 1 cut(s) 245
AluI AGCT 1 cut(s) 245
Alw26I GTCTC 3 cut(s) 360, 385, 391
ApeKI GCWGC 1 cut(s) 68
AspLEI GCGC 1 cut(s) 115
AspS9I GGNCC 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 271, 301
AvaII GGWCC 1 cut(s) 136
BbsI GAAGAC 1 cut(s) 248
BbvI GCAGC 1 cut(s) 80
BccI CCATC 2 cut(s) 81, 301
BceAI ACGGC 1 cut(s) 54
BciT130I CCWGG 1 cut(s) 219
BciVI GTATCC 1 cut(s) 40
BcoDI GTCTC 3 cut(s) 360, 385, 391
BfaI CTAG 1 cut(s) 140
BfmI CTRYAG 1 cut(s) 435
BfuI GTATCC 1 cut(s) 40
BisI GCNGC 2 cut(s) 69, 151
BlsI GCNGC 2 cut(s) 70, 152
Bme1390I CCNGG 1 cut(s) 219
Bme18I GGWCC 1 cut(s) 136
BmgBI CACGTC 2 cut(s) 370, 401
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 16
BmrFI CCNGG 1 cut(s) 219
BpiI GAAGAC 1 cut(s) 248
BpmI CTGGAG 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 80
BsaHI GRCGYC 2 cut(s) 41, 153
BsaJI CCNNGG 3 cut(s) 60, 217, 234
BsaWI WCCGGW 2 cut(s) 175, 281
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse1I ACTGG 2 cut(s) 88, 185
Bse3DI GCAATG 1 cut(s) 194
BseBI CCWGG 1 cut(s) 219
BseDI CCNNGG 3 cut(s) 60, 217, 234
BseGI GGATG 1 cut(s) 318
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMI GCAATG 1 cut(s) 194
BseNI ACTGG 2 cut(s) 88, 185
BseRI GAGGAG 1 cut(s) 237
BseXI GCAGC 1 cut(s) 80
Bsh1236I CGCG 1 cut(s) 113
Bsh1285I CGRYCG 1 cut(s) 325
BsiEI CGRYCG 1 cut(s) 325
BsiSI CCGG 3 cut(s) 176, 282, 328
BslI CCNNNNNNNGG 1 cut(s) 223
BsmAI GTCTC 3 cut(s) 360, 385, 391
BsmBI CGTCTC 2 cut(s) 360, 391
Bsp143I GATC 2 cut(s) 322, 427
BspACI CCGC 2 cut(s) 52, 150
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 1 cut(s) 16
BspMAI CTGCAG 1 cut(s) 439
BsrDI GCAATG 1 cut(s) 194
BsrI ACTGG 2 cut(s) 88, 185
BssECI CCNNGG 3 cut(s) 60, 217, 234
BssMI GATC 2 cut(s) 322, 427
BssNI GRCGYC 2 cut(s) 41, 153
BssT1I CCWWGG 2 cut(s) 60, 234
Bst2UI CCWGG 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 8
BstACI GRCGYC 2 cut(s) 41, 153
BstF5I GGATG 1 cut(s) 318
BstFNI CGCG 1 cut(s) 113
BstHHI GCGC 1 cut(s) 115
BstKTI GATC 2 cut(s) 325, 430
BstMAI GTCTC 3 cut(s) 360, 385, 391
BstMBI GATC 2 cut(s) 322, 427
BstMCI CGRYCG 1 cut(s) 325
BstMWI GCNNNNNNNGC 2 cut(s) 65, 156
BstNI CCWGG 1 cut(s) 219
BstSCI CCNGG 1 cut(s) 217
BstSFI CTRYAG 1 cut(s) 435
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 1 cut(s) 80
BstV2I GAAGAC 1 cut(s) 248
BsuI GTATCC 1 cut(s) 40
BtrI CACGTC 2 cut(s) 370, 401
BtsCI GGATG 1 cut(s) 318
CfoI GCGC 1 cut(s) 115
Cfr13I GGNCC 1 cut(s) 136
CseI GACGC 4 cut(s) 30, 74, 119, 142
CviJI RGCY 5 cut(s) 15, 71, 216, 231, 245
CviKI_1 RGCY 5 cut(s) 15, 71, 216, 231, 245
DpnI GATC 2 cut(s) 324, 429
DpnII GATC 2 cut(s) 322, 427
Eco130I CCWWGG 2 cut(s) 60, 234
Eco47I GGWCC 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 217
EcoT14I CCWWGG 2 cut(s) 60, 234
ErhI CCWWGG 2 cut(s) 60, 234
Esp3I CGTCTC 2 cut(s) 360, 391
FaiI YATR 2 cut(s) 166, 275
FauI CCCGC 1 cut(s) 59
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 2 cut(s) 69, 151
FokI GGATG 1 cut(s) 325
Fsp4HI GCNGC 2 cut(s) 69, 151
FspBI CTAG 1 cut(s) 140
GlaI GCGC 1 cut(s) 114
GluI GCNGC 2 cut(s) 69, 151
GsuI CTGGAG 1 cut(s) 168
HapII CCGG 3 cut(s) 176, 282, 328
HgaI GACGC 4 cut(s) 30, 74, 119, 142
HhaI GCGC 1 cut(s) 115
Hin1I GRCGYC 2 cut(s) 41, 153
Hin6I GCGC 1 cut(s) 113
HinP1I GCGC 1 cut(s) 113
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 2 cut(s) 161, 179
HpaII CCGG 3 cut(s) 176, 282, 328
HphI GGTGA 2 cut(s) 271, 301
Hpy166II GTNNAC 2 cut(s) 25, 130
Hpy188I TCNGA 2 cut(s) 46, 192
Hpy8I GTNNAC 2 cut(s) 25, 130
Hpy99I CGWCG 5 cut(s) 46, 113, 158, 209, 255
HpyAV CCTTC 1 cut(s) 203
HpyCH4III ACNGT 1 cut(s) 8
HpyCH4IV ACGT 2 cut(s) 369, 400
HpyCH4V TGCA 2 cut(s) 120, 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 156
HpySE526I ACGT 2 cut(s) 369, 400
Hsp92I GRCGYC 2 cut(s) 41, 153
HspAI GCGC 1 cut(s) 113
Kzo9I GATC 2 cut(s) 322, 427
LmnI GCTCC 2 cut(s) 20, 250
LpnPI CCDG 7 cut(s) 101, 189, 198, 204, 231, 295, 341
Lsp1109I GCAGC 1 cut(s) 80
MaeI CTAG 1 cut(s) 140
MaeII ACGT 2 cut(s) 369, 400
MaeIII GTNAC 1 cut(s) 277
MalI GATC 2 cut(s) 324, 429
MboI GATC 2 cut(s) 322, 427
MboII GAAGA 1 cut(s) 248
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 24
MnlI CCTC 8 cut(s) 127, 211, 258, 329, 367, 373, 398, 404
MslI CAYNNNNRTG 1 cut(s) 284
MspI CCGG 3 cut(s) 176, 282, 328
MspR9I CCNGG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 219
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 2 cut(s) 65, 156
NdeII GATC 2 cut(s) 322, 427
NlaIV GGNNCC 1 cut(s) 16
NmuCI GTSAC 1 cut(s) 277
PcsI WCGNNNNNNNCGW 1 cut(s) 256
PfeI GAWTC 1 cut(s) 179
PflFI GACNNNGTC 1 cut(s) 140
PkrI GCNGC 2 cut(s) 70, 152
Ple19I CGATCG 1 cut(s) 325
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
PpuMI RGGWCCY 1 cut(s) 136
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 1 cut(s) 217
PspGI CCWGG 1 cut(s) 217
PspN4I GGNNCC 1 cut(s) 16
PspPI GGNCC 1 cut(s) 136
PspPPI RGGWCCY 1 cut(s) 136
PstI CTGCAG 1 cut(s) 439
PsyI GACNNNGTC 1 cut(s) 140
PvuI CGATCG 1 cut(s) 325
RseI CAYNNNNRTG 1 cut(s) 284
SatI GCNGC 2 cut(s) 69, 151
Sau3AI GATC 2 cut(s) 322, 427
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 1 cut(s) 219
SetI ASST 4 cut(s) 141, 247, 372, 403
SfcI CTRYAG 1 cut(s) 435
SinI GGWCC 1 cut(s) 136
SmiMI CAYNNNNRTG 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SsiI CCGC 2 cut(s) 52, 150
SspMI CTAG 1 cut(s) 140
StyD4I CCNGG 1 cut(s) 217
StyI CCWWGG 2 cut(s) 60, 234
TaaI ACNGT 1 cut(s) 8
TaiI ACGT 2 cut(s) 372, 403
TaqI TCGA 4 cut(s) 207, 259, 305, 321
TaqII GACCGA 1 cut(s) 420
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 1 cut(s) 179
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 277
TspGWI ACGGA 1 cut(s) 193
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 136
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.