Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000569.1

Orthogroup ID

OG0007674

Physical Location & Seq

Genomic Coordinates Forward (+)

1 .. 1877

Gene Structure 1877 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1392 bp

aagttaaagccattgcattgggataaagttagagctagctctgaccgtgaaatggtgtgggatcagctaagatcaagctctttcaaattgaatgaagaaatgatagagacattgttcgttgtaaatacacccaaaccgaatccaaaggaaacaaccccacgcccggttattccctcacctaagcaagagaacagagtactcgatcctaaaaagtcccagaatattgcaattttgcttagggcactcaatgtgaccatagaagaagtttgtgaagcgctcatagaaggtaatgcagatgctcttggaactgaattacttgaaagtctattgaagatggctccaaccaaagaagaagagcgtaagctgaaggagtgcaaagatgattcaccggttaagcttggccctgctgagaaatttctgaaagaacttcttgatgttccttttgcatttaaaagggtcgaggcaatgctttacatgactaattttgaatctgaaatcgaatacctgaaaaagtctttcgaaactctagaggctgcctgtgaagaattgagaaatagtaggatgttcttgaagcttttagaggctgtgctcaagactggaaacagaatgaatgttgggacaaaccgcggtgatgcccatgccttcaaacttgacacactcctcaagcttgccgatgttaagggagcagatgggaaaaccacgcttttgcattttgttgtacaagaaatcataagaactgaaggtgctcgtctcattggtggtcaaacttcatcaaacacagctttaaatgacgatgccaagtgtaggagacttggcctgccagttgtttcaggtctcagttcagacctcactaatgtcaagaaagctgctgccatggactccgatgtgcttagcggtgaagtttttaagctttctagaggtattagcaacattggggaggttgtgcaattagctgacagaaaggtgtcagatggaagcagcgggaaatttgcagagtccatgaccatgttcatgaaaatggccgaggaggagattataagacttcaagctcaagaaagtgttgctctgtccttggtgaaggagatcacggagtacttccatgggaattccgccagggaagaagctcatccatttcgaatcttcatggtagtgagagactttctgacagttcttgatcgggtatgtaaggaagttggtctgataaacgagcgaaccattgttagtaatgcccaaaagttcccagttccagtaaatcccatgctacagcaacaagtatttccagtaaacccaatgctaccgcaagttcatcccgtaaacccaatgctaccgcaagttctccctggaatccatggcaggcgaccctacgattcatccgatgatgagagtacgtaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009987 GO:0010638 GO:0010639 GO:0016020 GO:0016043 GO:0030029 GO:0030036 GO:0030312 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0030838 GO:0031333 GO:0031334 GO:0032271 GO:0032272 GO:0032273 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0043242 GO:0043244 GO:0043254 GO:0044087 GO:0044089 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051015 GO:0051016 GO:0051128 GO:0051129 GO:0051130 GO:0051493 GO:0051494 GO:0051495 GO:0051693 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0097435 GO:0110053 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04320 map04320

Pfam Domains

Protein Families

FH2

Protein Analysis

463

Amino Acids

51.82

Weight (kDa)

5.98

Isoelectric Point (pI)

44.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	1 - 391	1.3e-120	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007674)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25500
fragaria_vesca	FvH4_2g17360 FvH4_2g17360 FvH4_2g17360
malus_domestica	MD05G1152500.v1.1 MD10G1147000.v1.1
prunus_persica	Prupe.8G185400_v2.0.a1
pyrus_communis	pycom05g14690 pycom10g12670
rosa_chinensis	RchiOBHm_Chr6g0281991
rosa_laevigata	RLG00000012921
rosa_multiflora	Rmu_sc0000569.1_g000001 Rmu_sc0001255.1_g000001 Rmu_sc0011823.1_g000001
rosa_roxburghii	Rroxscaffold_7G00186650
rosa_rugosa	Rorug06G0144700
rosa_samantha	Rh4CG162200 Rh4CG270300 Rh6AG257900 Rh6BG260900 Rh6CG259800 Rh6DG252200 Rh7AG416400
rosa_wichuraiana	Rw6G022300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1037
AccII	CGCG	1 cut(s)	627
AciI	CCGC	7 cut(s)	625, 627, 894, 981, 1110, 1298, 1328
AclWI	GGATC	2 cut(s)	69, 197
AcoI	YGGCCR	1 cut(s)	1020
AcsI	RAATTY	3 cut(s)	413, 986, 1105
AcuI	CTGAAG	2 cut(s)	386, 759
AfaI	GTAC	4 cut(s)	198, 720, 1094, 1387
AfeI	AGCGCT	1 cut(s)	276
AfiI	CCNNNNNNNGG	3 cut(s)	52, 163, 804
AgeI	ACCGGT	1 cut(s)	388
AgsI	TTSAA	8 cut(s)	85, 91, 320, 331, 488, 571, 646, 1046
AjnI	CCWGG	2 cut(s)	1112, 1339
Alw21I	GWGCWC	2 cut(s)	591, 748
Alw26I	GTCTC	5 cut(s)	101, 755, 802, 839, 1149
AlwI	GGATC	2 cut(s)	69, 197
Aor51HI	AGCGCT	1 cut(s)	276
AoxI	GGCC	3 cut(s)	400, 814, 1020
ApeKI	GCWGC	4 cut(s)	533, 866, 869, 978
ApoI	RAATTY	3 cut(s)	413, 986, 1105
AsiGI	ACCGGT	1 cut(s)	388
AspLEI	GCGC	1 cut(s)	277
AspS9I	GGNCC	1 cut(s)	401
AsuC2I	CCSGG	1 cut(s)	164
AsuHPI	GGTGA	5 cut(s)	168, 378, 641, 908, 1087
AsuII	TTCGAA	2 cut(s)	519, 1135
AsuNHI	GCTAGC	1 cut(s)	35
BaeGI	GKGCMC	1 cut(s)	244
Bbv12I	GWGCWC	2 cut(s)	591, 748
BbvI	GCAGC	4 cut(s)	520, 853, 856, 990
BccI	CCATC	3 cut(s)	328, 683, 965
BciT130I	CCWGG	2 cut(s)	1114, 1341
BcnI	CCSGG	1 cut(s)	164
BcoDI	GTCTC	5 cut(s)	101, 755, 802, 839, 1149
BfaI	CTAG	3 cut(s)	36, 527, 915
BfmI	CTRYAG	1 cut(s)	1262
BfoI	RGCGCY	1 cut(s)	278
BisI	GCNGC	4 cut(s)	534, 867, 870, 979
BlpI	GCTNAGC	1 cut(s)	890
BlsI	GCNGC	4 cut(s)	535, 868, 871, 980
BmcAI	AGTACT	2 cut(s)	198, 1094
Bme1390I	CCNGG	3 cut(s)	164, 1114, 1341
BmgT120I	GGNCC	1 cut(s)	401
BmiI	GGNNCC	1 cut(s)	339
BmrFI	CCNGG	3 cut(s)	164, 1114, 1341
BmrI	ACTGGG	1 cut(s)	1235
BmsI	GCATC	3 cut(s)	286, 622, 784
BmtI	GCTAGC	1 cut(s)	39
BmuI	ACTGGG	1 cut(s)	1235
Bpu10I	CCTNAGC	2 cut(s)	180, 236
Bpu1102I	GCTNAGC	1 cut(s)	890
Bpu14I	TTCGAA	2 cut(s)	519, 1135
BpuEI	CTTGAG	3 cut(s)	575, 647, 1035
BpuMI	CCSGG	1 cut(s)	164
BsaAI	YACGTR	1 cut(s)	1389
BsaI	GGTCTC	1 cut(s)	839
BsaJI	CCNNGG	8 cut(s)	625, 873, 1023, 1071, 1099, 1113, 1339, 1348
BsaWI	WCCGGW	1 cut(s)	388
Bsc4I	CCNNNNNNNGG	3 cut(s)	52, 163, 804
Bse118I	RCCGGY	1 cut(s)	388
Bse1I	ACTGG	5 cut(s)	601, 821, 1241, 1247, 1280
Bse3DI	GCAATG	2 cut(s)	11, 471
BseBI	CCWGG	2 cut(s)	1114, 1341
BseDI	CCNNGG	8 cut(s)	625, 873, 1023, 1071, 1099, 1113, 1339, 1348
BseGI	GGATG	4 cut(s)	567, 1126, 1306, 1370
BseLI	CCNNNNNNNGG	3 cut(s)	52, 163, 804
BseMI	GCAATG	2 cut(s)	11, 471
BseMII	CTCAG	2 cut(s)	399, 850
BseNI	ACTGG	5 cut(s)	601, 821, 1241, 1247, 1280
BseRI	GAGGAG	3 cut(s)	650, 1040, 1043
BseSI	GKGCMC	1 cut(s)	244
BseXI	GCAGC	4 cut(s)	520, 853, 856, 990
Bsh1236I	CGCG	1 cut(s)	627
BshFI	GGCC	3 cut(s)	402, 816, 1022
BshTI	ACCGGT	1 cut(s)	388
BsiHKAI	GWGCWC	2 cut(s)	591, 748
BsiSI	CCGG	2 cut(s)	164, 389
BslFI	GGGAC	2 cut(s)	199, 631
BslI	CCNNNNNNNGG	3 cut(s)	52, 163, 804
BsmAI	GTCTC	5 cut(s)	101, 755, 802, 839, 1149
BsmBI	CGTCTC	1 cut(s)	755
BsmFI	GGGAC	2 cut(s)	199, 631
BsnI	GGCC	3 cut(s)	402, 816, 1022
Bso31I	GGTCTC	1 cut(s)	839
Bsp119I	TTCGAA	2 cut(s)	519, 1135
Bsp1286I	GDGCHC	3 cut(s)	244, 591, 748
Bsp1407I	TGTACA	1 cut(s)	718
Bsp143I	GATC	5 cut(s)	61, 71, 202, 1083, 1174
Bsp1720I	GCTNAGC	1 cut(s)	890
Bsp19I	CCATGG	3 cut(s)	873, 1099, 1348
BspACI	CCGC	7 cut(s)	625, 627, 894, 981, 1110, 1298, 1328
BspANI	GGCC	3 cut(s)	402, 816, 1022
BspCNI	CTCAG	2 cut(s)	400, 849
BspFNI	CGCG	1 cut(s)	627
BspHI	TCATGA	1 cut(s)	1011
BspLI	GGNNCC	1 cut(s)	339
BspOI	GCTAGC	1 cut(s)	39
BspPI	GGATC	2 cut(s)	69, 197
BspQI	GCTCTTC	1 cut(s)	348
BspT104I	TTCGAA	2 cut(s)	519, 1135
BspTNI	GGTCTC	1 cut(s)	839
BsrDI	GCAATG	2 cut(s)	11, 471
BsrFI	RCCGGY	1 cut(s)	388
BsrGI	TGTACA	1 cut(s)	718
BsrI	ACTGG	5 cut(s)	601, 821, 1241, 1247, 1280
BssAI	RCCGGY	1 cut(s)	388
BssECI	CCNNGG	8 cut(s)	625, 873, 1023, 1071, 1099, 1113, 1339, 1348
BssMI	GATC	5 cut(s)	61, 71, 202, 1083, 1174
BssT1I	CCWWGG	4 cut(s)	873, 1071, 1099, 1348
Bst2UI	CCWGG	2 cut(s)	1114, 1341
Bst4CI	ACNGT	2 cut(s)	47, 1168
Bst6I	CTCTTC	1 cut(s)	348
BstAUI	TGTACA	1 cut(s)	718
BstBAI	YACGTR	1 cut(s)	1389
BstBI	TTCGAA	2 cut(s)	519, 1135
BstC8I	GCNNGC	4 cut(s)	37, 669, 818, 1355
BstDEI	CTNAG	6 cut(s)	68, 180, 236, 408, 836, 890
BstDSI	CCRYGG	4 cut(s)	625, 873, 1099, 1348
BstF5I	GGATG	4 cut(s)	567, 1126, 1306, 1370
BstFNI	CGCG	1 cut(s)	627
BstH2I	RGCGCY	1 cut(s)	278
BstHHI	GCGC	1 cut(s)	277
BstKTI	GATC	5 cut(s)	64, 74, 205, 1086, 1177
BstMAI	GTCTC	5 cut(s)	101, 755, 802, 839, 1149
BstMBI	GATC	5 cut(s)	61, 71, 202, 1083, 1174
BstNI	CCWGG	2 cut(s)	1114, 1341
BstSCI	CCNGG	3 cut(s)	162, 1112, 1339
BstSFI	CTRYAG	1 cut(s)	1262
BstSLI	GKGCMC	1 cut(s)	244
BstSNI	TACGTA	1 cut(s)	1389
BstUI	CGCG	1 cut(s)	627
BstV1I	GCAGC	4 cut(s)	520, 853, 856, 990
BstXI	CCANNNNNNTGG	1 cut(s)	18
BsuRI	GGCC	3 cut(s)	402, 816, 1022
BtgI	CCRYGG	4 cut(s)	625, 873, 1099, 1348
BtsCI	GGATG	4 cut(s)	567, 1126, 1306, 1370
Cac8I	GCNNGC	4 cut(s)	37, 669, 818, 1355
CciI	TCATGA	1 cut(s)	1011
CfoI	GCGC	1 cut(s)	277
Cfr10I	RCCGGY	1 cut(s)	388
Cfr13I	GGNCC	1 cut(s)	401
Cfr42I	CCGCGG	1 cut(s)	628
Csp6I	GTAC	4 cut(s)	197, 719, 1093, 1386
CspAI	ACCGGT	1 cut(s)	388
CviQI	GTAC	4 cut(s)	197, 719, 1093, 1386
DdeI	CTNAG	6 cut(s)	68, 180, 236, 408, 836, 890
DpnI	GATC	5 cut(s)	63, 73, 204, 1085, 1176
DpnII	GATC	5 cut(s)	61, 71, 202, 1083, 1174
DraI	TTTAAA	2 cut(s)	451, 786
EaeI	YGGCCR	1 cut(s)	1020
Eam1104I	CTCTTC	1 cut(s)	348
EarI	CTCTTC	1 cut(s)	348
EciI	GGCGGA	1 cut(s)	1099
Eco105I	TACGTA	1 cut(s)	1389
Eco130I	CCWWGG	4 cut(s)	873, 1071, 1099, 1348
Eco31I	GGTCTC	1 cut(s)	839
Eco47III	AGCGCT	1 cut(s)	276
Eco57I	CTGAAG	2 cut(s)	386, 759
EcoRI	GAATTC	1 cut(s)	1105
EcoRII	CCWGG	2 cut(s)	1112, 1339
EcoT14I	CCWWGG	4 cut(s)	873, 1071, 1099, 1348
ErhI	CCWWGG	4 cut(s)	873, 1071, 1099, 1348
Esp3I	CGTCTC	1 cut(s)	755
FalI	AAGNNNNNCTT	2 cut(s)	414, 446
FaqI	GGGAC	2 cut(s)	199, 631
FauI	CCCGC	1 cut(s)	974
Fnu4HI	GCNGC	4 cut(s)	534, 867, 870, 979
FokI	GGATG	4 cut(s)	574, 1113, 1293, 1357
Fsp4HI	GCNGC	4 cut(s)	534, 867, 870, 979
FspBI	CTAG	3 cut(s)	36, 527, 915
GlaI	GCGC	1 cut(s)	276
GluI	GCNGC	4 cut(s)	534, 867, 870, 979
HaeII	RGCGCY	1 cut(s)	278
HaeIII	GGCC	3 cut(s)	402, 816, 1022
HapII	CCGG	2 cut(s)	164, 389
HhaI	GCGC	1 cut(s)	277
Hin6I	GCGC	1 cut(s)	275
HinP1I	GCGC	1 cut(s)	275
HindIII	AAGCTT	4 cut(s)	395, 572, 665, 908
HinfI	GANTC	8 cut(s)	139, 383, 488, 878, 995, 1137, 1344, 1367
HpaII	CCGG	2 cut(s)	164, 389
HphI	GGTGA	5 cut(s)	168, 378, 641, 908, 1087
Hpy166II	GTNNAC	2 cut(s)	1285, 1315
Hpy188I	TCNGA	9 cut(s)	43, 420, 493, 844, 883, 970, 1164, 1200, 1375
Hpy188III	TCNNGA	9 cut(s)	431, 527, 568, 592, 859, 915, 1012, 1052, 1172
Hpy8I	GTNNAC	2 cut(s)	1285, 1315
HpyAV	CCTTC	5 cut(s)	278, 361, 652, 734, 1072
HpyCH4III	ACNGT	2 cut(s)	47, 1168
HpyCH4IV	ACGT	1 cut(s)	1388
HpyCH4V	TGCA	8 cut(s)	16, 227, 293, 375, 446, 709, 946, 992
HpyF3I	CTNAG	6 cut(s)	68, 180, 236, 408, 836, 890
HpySE526I	ACGT	1 cut(s)	1388
HspAI	GCGC	1 cut(s)	275
KspI	CCGCGG	1 cut(s)	628
Kzo9I	GATC	5 cut(s)	61, 71, 202, 1083, 1174
LguI	GCTCTTC	1 cut(s)	348
LmnI	GCTCC	2 cut(s)	343, 683
Lsp1109I	GCAGC	4 cut(s)	520, 853, 856, 990
LweI	GCATC	3 cut(s)	286, 622, 784
MaeI	CTAG	3 cut(s)	36, 527, 915
MaeII	ACGT	1 cut(s)	1388
MaeIII	GTNAC	1 cut(s)	250
MalI	GATC	5 cut(s)	63, 73, 204, 1085, 1176
MboI	GATC	5 cut(s)	61, 71, 202, 1083, 1174
MboII	GAAGA	8 cut(s)	107, 272, 343, 362, 365, 554, 1130, 1132
MhlI	GDGCHC	3 cut(s)	244, 591, 748
MluCI	AATT	9 cut(s)	86, 228, 311, 413, 481, 545, 947, 986, 1105
MlyI	GAGTC	2 cut(s)	872, 1004
MmeI	TCCRAC	1 cut(s)	365
MseI	TTAA	6 cut(s)	5, 393, 450, 678, 785, 906
MslI	CAYNNNNRTG	4 cut(s)	1004, 1010, 1016, 1148
MspA1I	CMGCKG	2 cut(s)	627, 981
MspI	CCGG	2 cut(s)	164, 389
MspR9I	CCNGG	3 cut(s)	164, 1114, 1341
MvaI	CCWGG	2 cut(s)	1114, 1341
MvnI	CGCG	1 cut(s)	627
NciI	CCSGG	1 cut(s)	164
NcoI	CCATGG	3 cut(s)	873, 1099, 1348
NdeII	GATC	5 cut(s)	61, 71, 202, 1083, 1174
NheI	GCTAGC	1 cut(s)	35
NlaIV	GGNNCC	1 cut(s)	339
NmeAIII	GCCGAG	1 cut(s)	1048
NmuCI	GTSAC	1 cut(s)	250
NspV	TTCGAA	2 cut(s)	519, 1135
PagI	TCATGA	1 cut(s)	1011
PciSI	GCTCTTC	1 cut(s)	348
PcsI	WCGNNNNNNNCGW	1 cut(s)	1371
PfeI	GAWTC	6 cut(s)	139, 383, 488, 1137, 1344, 1367
PinAI	ACCGGT	1 cut(s)	388
PkrI	GCNGC	4 cut(s)	535, 868, 871, 980
PleI	GAGTC	2 cut(s)	872, 1003
PpsI	GAGTC	2 cut(s)	872, 1003
Ppu21I	YACGTR	1 cut(s)	1389
PsiI	TTATAA	1 cut(s)	1037
Psp6I	CCWGG	2 cut(s)	1112, 1339
PspGI	CCWGG	2 cut(s)	1112, 1339
PspN4I	GGNNCC	1 cut(s)	339
PspPI	GGNCC	1 cut(s)	401
RsaI	GTAC	4 cut(s)	198, 720, 1094, 1387
RsaNI	GTAC	4 cut(s)	197, 719, 1093, 1386
RseI	CAYNNNNRTG	4 cut(s)	1004, 1010, 1016, 1148
SacII	CCGCGG	1 cut(s)	628
SapI	GCTCTTC	1 cut(s)	348
SaqAI	TTAA	6 cut(s)	5, 393, 450, 678, 785, 906
SatI	GCNGC	4 cut(s)	534, 867, 870, 979
Sau3AI	GATC	5 cut(s)	61, 71, 202, 1083, 1174
Sau96I	GGNCC	1 cut(s)	401
ScaI	AGTACT	2 cut(s)	198, 1094
SchI	GAGTC	2 cut(s)	872, 1004
ScrFI	CCNGG	3 cut(s)	164, 1114, 1341
SduI	GDGCHC	3 cut(s)	244, 591, 748
SfaNI	GCATC	3 cut(s)	286, 622, 784
SfcI	CTRYAG	1 cut(s)	1262
Sfr303I	CCGCGG	1 cut(s)	628
SfuI	TTCGAA	2 cut(s)	519, 1135
SgrBI	CCGCGG	1 cut(s)	628
SmiMI	CAYNNNNRTG	4 cut(s)	1004, 1010, 1016, 1148
SmlI	CTYRAG	3 cut(s)	590, 662, 1050
SmoI	CTYRAG	3 cut(s)	590, 662, 1050
SnaBI	TACGTA	1 cut(s)	1389
Sse9I	AATT	9 cut(s)	86, 228, 311, 413, 481, 545, 947, 986, 1105
SsiI	CCGC	7 cut(s)	625, 627, 894, 981, 1110, 1298, 1328
SspI	AATATT	1 cut(s)	223
SspMI	CTAG	3 cut(s)	36, 527, 915
StyD4I	CCNGG	3 cut(s)	162, 1112, 1339
StyI	CCWWGG	4 cut(s)	873, 1071, 1099, 1348
TaaI	ACNGT	2 cut(s)	47, 1168
TaiI	ACGT	1 cut(s)	1391
TaqI	TCGA	5 cut(s)	201, 459, 498, 519, 1135
TasI	AATT	9 cut(s)	86, 228, 311, 413, 481, 545, 947, 986, 1105
TatI	WGTACW	3 cut(s)	196, 718, 1092
TfiI	GAWTC	6 cut(s)	139, 383, 488, 1137, 1344, 1367
Tru1I	TTAA	6 cut(s)	5, 393, 450, 678, 785, 906
Tru9I	TTAA	6 cut(s)	5, 393, 450, 678, 785, 906
TseFI	GTSAC	1 cut(s)	250
TseI	GCWGC	4 cut(s)	533, 866, 869, 978
Tsp45I	GTSAC	1 cut(s)	250
TspDTI	ATGAA	8 cut(s)	108, 623, 759, 1000, 1028, 1132, 1295, 1359
TspGWI	ACGGA	1 cut(s)	1103
XapI	RAATTY	3 cut(s)	413, 986, 1105
XbaI	TCTAGA	2 cut(s)	526, 914
XspI	CTAG	3 cut(s)	36, 527, 915
ZrmI	AGTACT	2 cut(s)	198, 1094

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.