Rmu_sc0001272.1_g000011

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001272.1
OG0010391
Physical Location & Seq
Forward (+)
54901 .. 60047
5147 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 951 bp
atgcggactggagcgggagctgacctgagaacaatcgagaaggttccctcgagtgttgacctggagtttgaccgttggctcgaggagaaggggggtaccatattcttgaagatggatgttgctgggaagtctagtggagcccaatatgagtgcttgctttttgatttggatgatactctgtaccccatgagctctggtctcaacttggcctgccggaagaacattgaagagtacatgctgcaaaacttgcaaattgaagaaagtgaggtatcaaggatgtgcctggaattgtacagagaacatgggacaaccatggccggtctaaaggctcttggtcacgaatttgatgatgatgagtttcatgcatatgtacatggaagactaccctatgatgcgttgaagcctgatctagtgttaagaaaccttctcttatctatgccacagagaaaaataatcttcactaacgctgacaaagcacatgcagctgaagttctcagcaggttgtgcttggaagattgttttgaaggcatcatatgctttgaaactcttaaccctcctcgtcttcgaccagttgatttcattactgtaccagatgatgatgttatagagcatgctccagaggctactgccactgatcgcaccggaaataagtcaccaattctgtgcaaaccctctgttgaagcaattgaagctgctattcgtatagcaaatgttgacccacaaaaaactattttctttgatgacagtgctcgaaacatagcaagtgggaaggcagcaggacttcatacagttattgtcgggagttcagagctagtgcctggtgcagattatgtgctgcgtagcatacataacatcagagaagcaatacctgagatatgggaaggggaagaagggcaacaactagagcaatttatccagtctactgcagttgaaacagtgatccttgcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 map00760
Pfam Domains
Protein Families
HAD_2 Hydrolase_like

Protein Analysis

316

Amino Acids

35.12

Weight (kDa)

4.69

Isoelectric Point (pI)

55.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 50 - 260 2e-09 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 489
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 1 cut(s) 95
AccBSI CCGCTC 1 cut(s) 14
AccI GTMKAC 1 cut(s) 920
AciI CCGC 2 cut(s) 4, 14
AclWI GGATC 1 cut(s) 934
AcoI YGGCCR 1 cut(s) 315
AcsI RAATTY 1 cut(s) 341
AcuI CTGAAG 1 cut(s) 507
AfaI GTAC 6 cut(s) 97, 182, 233, 293, 372, 588
AgsI TTSAA 9 cut(s) 109, 227, 257, 400, 524, 542, 680, 689, 932
AjnI CCWGG 3 cut(s) 60, 282, 817
AluBI AGCT 5 cut(s) 20, 192, 485, 692, 811
AluI AGCT 5 cut(s) 20, 192, 485, 692, 811
Alw21I GWGCWC 2 cut(s) 194, 751
Alw26I GTCTC 1 cut(s) 203
AlwI GGATC 1 cut(s) 934
Ama87I CYCGRG 2 cut(s) 49, 80
AoxI GGCC 2 cut(s) 207, 315
ApeKI GCWGC 5 cut(s) 238, 482, 692, 773, 835
ApoI RAATTY 1 cut(s) 341
ArsI GACNNNNNNTTYG 2 cut(s) 50, 82
Asp718I GGTACC 1 cut(s) 95
AsuHPI GGTGA 1 cut(s) 645
AvaI CYCGRG 2 cut(s) 49, 80
BanI GGYRCC 1 cut(s) 95
BanII GRGCYC 2 cut(s) 142, 194
BbsI GAAGAC 2 cut(s) 385, 554
Bbv12I GWGCWC 2 cut(s) 194, 751
BbvI GCAGC 5 cut(s) 225, 494, 679, 785, 822
BccI CCATC 1 cut(s) 106
BciT130I CCWGG 3 cut(s) 62, 284, 819
BcoDI GTCTC 1 cut(s) 203
BfaI CTAG 4 cut(s) 132, 410, 812, 902
BfmI CTRYAG 1 cut(s) 924
BfuAI ACCTGC 1 cut(s) 489
BisI GCNGC 5 cut(s) 239, 483, 693, 774, 836
BlsI GCNGC 5 cut(s) 240, 484, 694, 775, 837
Bme1390I CCNGG 3 cut(s) 62, 284, 819
BmeT110I CYCGRG 2 cut(s) 49, 80
BmiI GGNNCC 3 cut(s) 45, 97, 139
BmrFI CCNGG 3 cut(s) 62, 284, 819
BmsI GCATC 2 cut(s) 382, 537
BpiI GAAGAC 2 cut(s) 385, 554
BpmI CTGGAG 3 cut(s) 30, 83, 600
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 312
BsaWI WCCGGW 1 cut(s) 641
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 3 cut(s) 13, 569, 916
BseBI CCWGG 3 cut(s) 62, 284, 819
BseDI CCNNGG 1 cut(s) 312
BseGI GGATG 3 cut(s) 121, 175, 282
BseMII CTCAG 3 cut(s) 17, 508, 861
BseNI ACTGG 3 cut(s) 13, 569, 916
BseRI GAGGAG 2 cut(s) 98, 546
BseXI GCAGC 5 cut(s) 225, 494, 679, 785, 822
BseYI CCCAGC 1 cut(s) 122
BsgI GTGCAG 1 cut(s) 843
BshFI GGCC 2 cut(s) 209, 317
BshNI GGYRCC 1 cut(s) 95
BsiHKAI GWGCWC 2 cut(s) 194, 751
BsiHKCI CYCGRG 2 cut(s) 49, 80
BsiSI CCGG 3 cut(s) 214, 318, 642
BslFI GGGAC 1 cut(s) 319
BsmAI GTCTC 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 319
BsnI GGCC 2 cut(s) 209, 317
Bso31I GGTCTC 1 cut(s) 203
BsoBI CYCGRG 2 cut(s) 49, 80
Bsp1286I GDGCHC 3 cut(s) 142, 194, 751
Bsp1407I TGTACA 2 cut(s) 291, 370
Bsp143I GATC 3 cut(s) 406, 634, 939
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 2 cut(s) 4, 14
BspANI GGCC 2 cut(s) 209, 317
BspCNI CTCAG 3 cut(s) 18, 507, 862
BspLI GGNNCC 3 cut(s) 45, 97, 139
BspMAI CTGCAG 1 cut(s) 928
BspMI ACCTGC 1 cut(s) 489
BspPI GGATC 1 cut(s) 934
BspT107I GGYRCC 1 cut(s) 95
BspTNI GGTCTC 1 cut(s) 203
BsrBI CCGCTC 1 cut(s) 14
BsrFI RCCGGY 1 cut(s) 317
BsrGI TGTACA 2 cut(s) 291, 370
BsrI ACTGG 3 cut(s) 13, 569, 916
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 3 cut(s) 406, 634, 939
BssT1I CCWWGG 1 cut(s) 312
Bst2UI CCWGG 3 cut(s) 62, 284, 819
Bst4CI ACNGT 5 cut(s) 74, 586, 746, 790, 937
Bst6I CTCTTC 1 cut(s) 222
BstAPI GCANNNNNTGC 3 cut(s) 247, 504, 534
BstAUI TGTACA 2 cut(s) 291, 370
BstC8I GCNNGC 3 cut(s) 155, 211, 612
BstDEI CTNAG 3 cut(s) 26, 494, 870
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 3 cut(s) 121, 175, 282
BstKTI GATC 3 cut(s) 409, 637, 942
BstMAI GTCTC 1 cut(s) 203
BstMBI GATC 3 cut(s) 406, 634, 939
BstMWI GCNNNNNNNGC 7 cut(s) 247, 473, 482, 504, 534, 620, 689
BstNI CCWGG 3 cut(s) 62, 284, 819
BstNSI RCATGY 3 cut(s) 238, 482, 614
BstSCI CCNGG 3 cut(s) 60, 282, 817
BstSFI CTRYAG 1 cut(s) 924
BstV1I GCAGC 5 cut(s) 225, 494, 679, 785, 822
BstV2I GAAGAC 2 cut(s) 385, 554
BsuRI GGCC 2 cut(s) 209, 317
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 3 cut(s) 121, 175, 282
BtsIMutI CAGTG 3 cut(s) 630, 751, 942
BveI ACCTGC 1 cut(s) 489
Cac8I GCNNGC 3 cut(s) 155, 211, 612
Cfr10I RCCGGY 1 cut(s) 317
Csp6I GTAC 6 cut(s) 96, 181, 232, 292, 371, 587
CviAII CATG 8 cut(s) 187, 235, 302, 313, 362, 374, 479, 611
CviQI GTAC 6 cut(s) 96, 181, 232, 292, 371, 587
DdeI CTNAG 3 cut(s) 26, 494, 870
DpnI GATC 3 cut(s) 408, 636, 941
DpnII GATC 3 cut(s) 406, 634, 939
EaeI YGGCCR 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
Ecl136II GAGCTC 1 cut(s) 192
Eco130I CCWWGG 1 cut(s) 312
Eco24I GRGCYC 2 cut(s) 142, 194
Eco31I GGTCTC 1 cut(s) 203
Eco53kI GAGCTC 1 cut(s) 192
Eco57I CTGAAG 1 cut(s) 507
Eco88I CYCGRG 2 cut(s) 49, 80
EcoICRI GAGCTC 1 cut(s) 192
EcoRII CCWGG 3 cut(s) 60, 282, 817
EcoT14I CCWWGG 1 cut(s) 312
EcoT22I ATGCAT 1 cut(s) 367
EcoT38I GRGCYC 2 cut(s) 142, 194
ErhI CCWWGG 1 cut(s) 312
FaeI CATG 8 cut(s) 190, 238, 305, 316, 365, 377, 482, 614
FaqI GGGAC 1 cut(s) 319
FatI CATG 8 cut(s) 186, 234, 301, 312, 361, 373, 478, 610
FauI CCCGC 1 cut(s) 7
FauNDI CATATG 2 cut(s) 367, 533
FblI GTMKAC 1 cut(s) 920
Fnu4HI GCNGC 5 cut(s) 239, 483, 693, 774, 836
FokI GGATG 3 cut(s) 128, 182, 289
FriOI GRGCYC 2 cut(s) 142, 194
Fsp4HI GCNGC 5 cut(s) 239, 483, 693, 774, 836
FspBI CTAG 4 cut(s) 132, 410, 812, 902
GluI GCNGC 5 cut(s) 239, 483, 693, 774, 836
GsaI CCCAGC 1 cut(s) 126
GsuI CTGGAG 3 cut(s) 30, 83, 600
HaeIII GGCC 2 cut(s) 209, 317
HapII CCGG 3 cut(s) 214, 318, 642
Hin1II CATG 8 cut(s) 190, 238, 305, 316, 365, 377, 482, 614
HincII GTYRAC 2 cut(s) 58, 715
HindII GTYRAC 2 cut(s) 58, 715
HpaII CCGG 3 cut(s) 214, 318, 642
HphI GGTGA 1 cut(s) 645
Hpy166II GTNNAC 3 cut(s) 58, 715, 921
Hpy188I TCNGA 2 cut(s) 808, 857
Hpy188III TCNNGA 5 cut(s) 37, 106, 338, 617, 799
Hpy8I GTNNAC 3 cut(s) 58, 715, 921
HpyAV CCTTC 7 cut(s) 34, 82, 434, 518, 763, 875, 884
HpyCH4III ACNGT 5 cut(s) 74, 586, 746, 790, 937
HpyCH4V TGCA 8 cut(s) 241, 250, 365, 482, 666, 824, 926, 947
HpyF10VI GCNNNNNNNGC 7 cut(s) 247, 473, 482, 504, 534, 620, 689
HpyF3I CTNAG 3 cut(s) 26, 494, 870
Hsp92II CATG 8 cut(s) 190, 238, 305, 316, 365, 377, 482, 614
KpnI GGTACC 1 cut(s) 99
Kzo9I GATC 3 cut(s) 406, 634, 939
LmnI GCTCC 4 cut(s) 11, 17, 137, 619
Lsp1109I GCAGC 5 cut(s) 225, 494, 679, 785, 822
LweI GCATC 2 cut(s) 382, 537
MaeI CTAG 4 cut(s) 132, 410, 812, 902
MaeIII GTNAC 2 cut(s) 335, 651
MalI GATC 3 cut(s) 408, 636, 941
MbiI CCGCTC 1 cut(s) 14
MboI GATC 3 cut(s) 406, 634, 939
MboII GAAGA 9 cut(s) 121, 229, 239, 269, 390, 448, 524, 554, 899
MfeI CAATTG 1 cut(s) 684
MhlI GDGCHC 3 cut(s) 142, 194, 751
MluCI AATT 6 cut(s) 252, 287, 341, 657, 684, 908
MnlI CCTC 7 cut(s) 58, 76, 259, 564, 567, 613, 682
Mph1103I ATGCAT 1 cut(s) 367
MseI TTAA 2 cut(s) 416, 549
MslI CAYNNNNRTG 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 3 cut(s) 214, 318, 642
MspR9I CCNGG 3 cut(s) 62, 284, 819
MunI CAATTG 1 cut(s) 684
MvaI CCWGG 3 cut(s) 62, 284, 819
MwoI GCNNNNNNNGC 7 cut(s) 247, 473, 482, 504, 534, 620, 689
NcoI CCATGG 1 cut(s) 312
NdeI CATATG 2 cut(s) 367, 533
NdeII GATC 3 cut(s) 406, 634, 939
NlaIII CATG 8 cut(s) 190, 238, 305, 316, 365, 377, 482, 614
NlaIV GGNNCC 3 cut(s) 45, 97, 139
NmuCI GTSAC 2 cut(s) 335, 651
NsiI ATGCAT 1 cut(s) 367
NspI RCATGY 3 cut(s) 238, 482, 614
PaeI GCATGC 1 cut(s) 614
PaeR7I CTCGAG 2 cut(s) 49, 80
PkrI GCNGC 5 cut(s) 240, 484, 694, 775, 837
Psp124BI GAGCTC 1 cut(s) 194
Psp6I CCWGG 3 cut(s) 60, 282, 817
PspFI CCCAGC 1 cut(s) 122
PspGI CCWGG 3 cut(s) 60, 282, 817
PspN4I GGNNCC 3 cut(s) 45, 97, 139
PspXI VCTCGAGB 2 cut(s) 49, 80
PstI CTGCAG 1 cut(s) 928
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 6 cut(s) 97, 182, 233, 293, 372, 588
RsaNI GTAC 6 cut(s) 96, 181, 232, 292, 371, 587
RseI CAYNNNNRTG 1 cut(s) 366
SacI GAGCTC 1 cut(s) 194
SaqAI TTAA 2 cut(s) 416, 549
SatI GCNGC 5 cut(s) 239, 483, 693, 774, 836
Sau3AI GATC 3 cut(s) 406, 634, 939
ScrFI CCNGG 3 cut(s) 62, 284, 819
SduI GDGCHC 3 cut(s) 142, 194, 751
SfaNI GCATC 2 cut(s) 382, 537
SfcI CTRYAG 1 cut(s) 924
Sfr274I CTCGAG 2 cut(s) 49, 80
SlaI CTCGAG 2 cut(s) 49, 80
SmiMI CAYNNNNRTG 1 cut(s) 366
SmlI CTYRAG 2 cut(s) 49, 80
SmoI CTYRAG 2 cut(s) 49, 80
SphI GCATGC 1 cut(s) 614
Sse9I AATT 6 cut(s) 252, 287, 341, 657, 684, 908
SsiI CCGC 2 cut(s) 4, 14
SspMI CTAG 4 cut(s) 132, 410, 812, 902
SstI GAGCTC 1 cut(s) 194
StyD4I CCNGG 3 cut(s) 60, 282, 817
StyI CCWWGG 1 cut(s) 312
TaaI ACNGT 5 cut(s) 74, 586, 746, 790, 937
TaqI TCGA 5 cut(s) 36, 50, 81, 565, 751
TasI AATT 6 cut(s) 252, 287, 341, 657, 684, 908
TatI WGTACW 3 cut(s) 231, 291, 370
Tru1I TTAA 2 cut(s) 416, 549
Tru9I TTAA 2 cut(s) 416, 549
TscAI CASTG 3 cut(s) 637, 751, 942
TseFI GTSAC 2 cut(s) 335, 651
TseI GCWGC 5 cut(s) 238, 482, 692, 773, 835
Tsp45I GTSAC 2 cut(s) 335, 651
TspDTI ATGAA 3 cut(s) 350, 568, 773
TspRI CASTG 3 cut(s) 637, 751, 942
XapI RAATTY 1 cut(s) 341
XceI RCATGY 3 cut(s) 238, 482, 614
XhoI CTCGAG 2 cut(s) 49, 80
XmiI GTMKAC 1 cut(s) 920
XspI CTAG 4 cut(s) 132, 410, 812, 902
Zsp2I ATGCAT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.