Rh3BG304300

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0010391
Physical Location & Seq
Reverse (-)
30712860 .. 30714684
1825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG304300.1

Sequence Viewer

Length: 852 bp
ATGGATGTTGCTGGGAAGTCTAGTGGAGCCAAATATGAGTGCTTGCTTTTTGATTTGGATGATACTCTGTACCCCATGAGCTCTGGTCTCAACTTGGCCTGCCGGAAGAACATTGAAGAGTACATGCTGCAACACTTGCAAATTGAAGAAAGTGAGGTATCAAGGATGTGCCTGGAATTGTACAGAGAGCATGGGACAACCATGGCCGGTCTAAAGGCTCTTGGTCACGAATTTGATGATGATGAGTTTCATGCATATGTCCATGGAAGACTACCCTATGATGCGTTGAAGCCTGATCTAGTGTTAAGAAACCTTCTCTTATCTATGCCACAGAGAAAAATAATCTTCACTAACGCTGACAAAGCACATGCAGCTGAAGTTCTCAGCAGGTTGTGCTTGGAAGATTGTTTTGAAGGCATCATATGCTTCGAAACTCTTAACCCTCCTCGTCTTCAACCAGTTGATTTCATTACTGTACCAGATGACGATGTTATAGAGCATGCTCCAGAGTCTACTGCCACTGATTGCACCGGAAATAATCTCAGTCCCAAGTCACCAATTCTGTGCAAACCCTCTGTTGAAGCAATTGAAGCTGCTATTCGTATAGCAAATGTTGACCCACAAAAAACTATTTTCTTTGATGACAGTGCTCGAAACATAGCAAGTGGGAAGGCAGCAGGACTTCATACAGTTATTGTCGGGAGTTCAGAGCTAGTGCCTGGTGCAGATTATGTGCTGCGTAGCATACATAACATCAGAGAAGCAATACCTGAGATATGGGAAGGGGAAGAAGGGCAACAACTAGAGCACTTTATCCAGTCTACTGCAGTTGAAACAGTGATCCTTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 map00760
Pfam Domains
Protein Families
HAD_2 Hydrolase_like

Protein Analysis

283

Amino Acids

31.35

Weight (kDa)

4.68

Isoelectric Point (pI)

57.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 13 - 227 4.3e-09 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 378
AccI GTMKAC 2 cut(s) 512, 821
AclWI GGATC 1 cut(s) 835
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 230
AcuI CTGAAG 1 cut(s) 396
AfaI GTAC 4 cut(s) 71, 122, 182, 477
AgsI TTSAA 8 cut(s) 116, 146, 289, 413, 455, 581, 590, 833
AjnI CCWGG 2 cut(s) 171, 718
AluBI AGCT 4 cut(s) 81, 374, 593, 712
AluI AGCT 4 cut(s) 81, 374, 593, 712
Alw21I GWGCWC 3 cut(s) 83, 652, 810
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 1 cut(s) 835
AoxI GGCC 2 cut(s) 96, 204
ApeKI GCWGC 5 cut(s) 127, 371, 593, 674, 736
ApoI RAATTY 1 cut(s) 230
AsuHPI GGTGA 1 cut(s) 546
AsuII TTCGAA 1 cut(s) 429
BanII GRGCYC 1 cut(s) 83
BbsI GAAGAC 2 cut(s) 274, 443
Bbv12I GWGCWC 3 cut(s) 83, 652, 810
BbvI GCAGC 5 cut(s) 114, 383, 580, 686, 723
BciT130I CCWGG 2 cut(s) 173, 720
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 4 cut(s) 21, 299, 713, 803
BfmI CTRYAG 1 cut(s) 825
BfuAI ACCTGC 1 cut(s) 378
BisI GCNGC 5 cut(s) 128, 372, 594, 675, 737
BlsI GCNGC 5 cut(s) 129, 373, 595, 676, 738
Bme1390I CCNGG 2 cut(s) 173, 720
BmiI GGNNCC 1 cut(s) 28
BmrFI CCNGG 2 cut(s) 173, 720
BmsI GCATC 2 cut(s) 271, 426
BpiI GAAGAC 2 cut(s) 274, 443
BpmI CTGGAG 1 cut(s) 489
Bpu14I TTCGAA 1 cut(s) 429
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 2 cut(s) 201, 262
BsaWI WCCGGW 1 cut(s) 530
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 2 cut(s) 458, 817
BseBI CCWGG 2 cut(s) 173, 720
BseDI CCNNGG 2 cut(s) 201, 262
BseGI GGATG 3 cut(s) 10, 64, 171
BseMII CTCAG 3 cut(s) 397, 556, 762
BseNI ACTGG 2 cut(s) 458, 817
BseRI GAGGAG 1 cut(s) 435
BseXI GCAGC 5 cut(s) 114, 383, 580, 686, 723
BseYI CCCAGC 1 cut(s) 11
BsgI GTGCAG 1 cut(s) 744
BshFI GGCC 2 cut(s) 98, 206
BsiHKAI GWGCWC 3 cut(s) 83, 652, 810
BsiSI CCGG 3 cut(s) 103, 207, 531
BslFI GGGAC 2 cut(s) 208, 531
BsmAI GTCTC 1 cut(s) 92
BsmFI GGGAC 2 cut(s) 208, 531
BsnI GGCC 2 cut(s) 98, 206
Bso31I GGTCTC 1 cut(s) 92
Bsp119I TTCGAA 1 cut(s) 429
Bsp1286I GDGCHC 3 cut(s) 83, 652, 810
Bsp1407I TGTACA 1 cut(s) 180
Bsp143I GATC 2 cut(s) 295, 840
Bsp19I CCATGG 2 cut(s) 201, 262
BspANI GGCC 2 cut(s) 98, 206
BspCNI CTCAG 3 cut(s) 396, 555, 763
BspLI GGNNCC 1 cut(s) 28
BspMAI CTGCAG 1 cut(s) 829
BspMI ACCTGC 1 cut(s) 378
BspPI GGATC 1 cut(s) 835
BspT104I TTCGAA 1 cut(s) 429
BspTNI GGTCTC 1 cut(s) 92
BsrFI RCCGGY 1 cut(s) 206
BsrGI TGTACA 1 cut(s) 180
BsrI ACTGG 2 cut(s) 458, 817
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 2 cut(s) 201, 262
BssMI GATC 2 cut(s) 295, 840
BssT1I CCWWGG 2 cut(s) 201, 262
Bst2UI CCWGG 2 cut(s) 173, 720
Bst4CI ACNGT 4 cut(s) 475, 647, 691, 838
Bst6I CTCTTC 1 cut(s) 111
BstAPI GCANNNNNTGC 3 cut(s) 136, 393, 423
BstAUI TGTACA 1 cut(s) 180
BstBI TTCGAA 1 cut(s) 429
BstC8I GCNNGC 3 cut(s) 44, 100, 501
BstDEI CTNAG 3 cut(s) 383, 542, 771
BstDSI CCRYGG 2 cut(s) 201, 262
BstF5I GGATG 3 cut(s) 10, 64, 171
BstKTI GATC 2 cut(s) 298, 843
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 2 cut(s) 295, 840
BstMWI GCNNNNNNNGC 6 cut(s) 136, 362, 371, 393, 423, 590
BstNI CCWGG 2 cut(s) 173, 720
BstNSI RCATGY 3 cut(s) 127, 371, 503
BstSCI CCNGG 2 cut(s) 171, 718
BstSFI CTRYAG 1 cut(s) 825
BstV1I GCAGC 5 cut(s) 114, 383, 580, 686, 723
BstV2I GAAGAC 2 cut(s) 274, 443
BsuRI GGCC 2 cut(s) 98, 206
BtgI CCRYGG 2 cut(s) 201, 262
BtsCI GGATG 3 cut(s) 10, 64, 171
BtsIMutI CAGTG 3 cut(s) 519, 652, 843
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 3 cut(s) 44, 100, 501
Cfr10I RCCGGY 1 cut(s) 206
Csp6I GTAC 4 cut(s) 70, 121, 181, 476
CviAII CATG 8 cut(s) 76, 124, 191, 202, 251, 263, 368, 500
CviJI RGCY 9 cut(s) 29, 81, 98, 206, 218, 292, 374, 593, 712
CviKI_1 RGCY 9 cut(s) 29, 81, 98, 206, 218, 292, 374, 593, 712
CviQI GTAC 4 cut(s) 70, 121, 181, 476
DdeI CTNAG 3 cut(s) 383, 542, 771
DpnI GATC 2 cut(s) 297, 842
DpnII GATC 2 cut(s) 295, 840
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Ecl136II GAGCTC 1 cut(s) 81
Eco130I CCWWGG 2 cut(s) 201, 262
Eco24I GRGCYC 1 cut(s) 83
Eco31I GGTCTC 1 cut(s) 92
Eco53kI GAGCTC 1 cut(s) 81
Eco57I CTGAAG 1 cut(s) 396
EcoICRI GAGCTC 1 cut(s) 81
EcoRII CCWGG 2 cut(s) 171, 718
EcoT14I CCWWGG 2 cut(s) 201, 262
EcoT22I ATGCAT 1 cut(s) 256
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 2 cut(s) 201, 262
FaeI CATG 8 cut(s) 79, 127, 194, 205, 254, 266, 371, 503
FaqI GGGAC 2 cut(s) 208, 531
FatI CATG 8 cut(s) 75, 123, 190, 201, 250, 262, 367, 499
FauNDI CATATG 2 cut(s) 256, 422
FblI GTMKAC 2 cut(s) 512, 821
Fnu4HI GCNGC 5 cut(s) 128, 372, 594, 675, 737
FokI GGATG 3 cut(s) 17, 71, 178
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 5 cut(s) 128, 372, 594, 675, 737
FspBI CTAG 4 cut(s) 21, 299, 713, 803
GluI GCNGC 5 cut(s) 128, 372, 594, 675, 737
GsaI CCCAGC 1 cut(s) 15
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 2 cut(s) 98, 206
HapII CCGG 3 cut(s) 103, 207, 531
Hin1II CATG 8 cut(s) 79, 127, 194, 205, 254, 266, 371, 503
HincII GTYRAC 1 cut(s) 616
HindII GTYRAC 1 cut(s) 616
HinfI GANTC 1 cut(s) 509
HpaII CCGG 3 cut(s) 103, 207, 531
HphI GGTGA 1 cut(s) 546
Hpy166II GTNNAC 3 cut(s) 513, 616, 822
Hpy188I TCNGA 2 cut(s) 709, 758
Hpy188III TCNNGA 3 cut(s) 227, 506, 700
Hpy8I GTNNAC 3 cut(s) 513, 616, 822
HpyAV CCTTC 5 cut(s) 323, 407, 664, 776, 785
HpyCH4III ACNGT 4 cut(s) 475, 647, 691, 838
HpyCH4V TGCA 9 cut(s) 130, 139, 254, 371, 528, 567, 725, 827, 848
HpyF10VI GCNNNNNNNGC 6 cut(s) 136, 362, 371, 393, 423, 590
HpyF3I CTNAG 3 cut(s) 383, 542, 771
Hsp92II CATG 8 cut(s) 79, 127, 194, 205, 254, 266, 371, 503
Kzo9I GATC 2 cut(s) 295, 840
LmnI GCTCC 2 cut(s) 26, 508
Lsp1109I GCAGC 5 cut(s) 114, 383, 580, 686, 723
LweI GCATC 2 cut(s) 271, 426
MaeI CTAG 4 cut(s) 21, 299, 713, 803
MaeIII GTNAC 2 cut(s) 224, 552
MalI GATC 2 cut(s) 297, 842
MboI GATC 2 cut(s) 295, 840
MboII GAAGA 8 cut(s) 118, 128, 158, 279, 337, 413, 443, 800
MfeI CAATTG 1 cut(s) 585
MhlI GDGCHC 3 cut(s) 83, 652, 810
MluCI AATT 5 cut(s) 141, 176, 230, 558, 585
MlyI GAGTC 1 cut(s) 518
MnlI CCTC 4 cut(s) 148, 453, 456, 583
Mph1103I ATGCAT 1 cut(s) 256
MseI TTAA 2 cut(s) 305, 438
MslI CAYNNNNRTG 1 cut(s) 255
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 3 cut(s) 103, 207, 531
MspR9I CCNGG 2 cut(s) 173, 720
MunI CAATTG 1 cut(s) 585
MvaI CCWGG 2 cut(s) 173, 720
MwoI GCNNNNNNNGC 6 cut(s) 136, 362, 371, 393, 423, 590
NcoI CCATGG 2 cut(s) 201, 262
NdeI CATATG 2 cut(s) 256, 422
NdeII GATC 2 cut(s) 295, 840
NlaIII CATG 8 cut(s) 79, 127, 194, 205, 254, 266, 371, 503
NlaIV GGNNCC 1 cut(s) 28
NmuCI GTSAC 2 cut(s) 224, 552
NsiI ATGCAT 1 cut(s) 256
NspI RCATGY 3 cut(s) 127, 371, 503
NspV TTCGAA 1 cut(s) 429
PaeI GCATGC 1 cut(s) 503
PkrI GCNGC 5 cut(s) 129, 373, 595, 676, 738
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
Psp124BI GAGCTC 1 cut(s) 83
Psp6I CCWGG 2 cut(s) 171, 718
PspFI CCCAGC 1 cut(s) 11
PspGI CCWGG 2 cut(s) 171, 718
PspN4I GGNNCC 1 cut(s) 28
PstI CTGCAG 1 cut(s) 829
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 4 cut(s) 71, 122, 182, 477
RsaNI GTAC 4 cut(s) 70, 121, 181, 476
RseI CAYNNNNRTG 1 cut(s) 255
SacI GAGCTC 1 cut(s) 83
SaqAI TTAA 2 cut(s) 305, 438
SatI GCNGC 5 cut(s) 128, 372, 594, 675, 737
Sau3AI GATC 2 cut(s) 295, 840
SchI GAGTC 1 cut(s) 518
ScrFI CCNGG 2 cut(s) 173, 720
SduI GDGCHC 3 cut(s) 83, 652, 810
SetI ASST 8 cut(s) 83, 159, 315, 376, 392, 595, 714, 772
SfaNI GCATC 2 cut(s) 271, 426
SfcI CTRYAG 1 cut(s) 825
SfuI TTCGAA 1 cut(s) 429
SmiMI CAYNNNNRTG 1 cut(s) 255
SphI GCATGC 1 cut(s) 503
Sse9I AATT 5 cut(s) 141, 176, 230, 558, 585
SspMI CTAG 4 cut(s) 21, 299, 713, 803
SstI GAGCTC 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 171, 718
StyI CCWWGG 2 cut(s) 201, 262
TaaI ACNGT 4 cut(s) 475, 647, 691, 838
TaqI TCGA 2 cut(s) 429, 652
TasI AATT 5 cut(s) 141, 176, 230, 558, 585
TatI WGTACW 2 cut(s) 120, 180
Tru1I TTAA 2 cut(s) 305, 438
Tru9I TTAA 2 cut(s) 305, 438
TscAI CASTG 3 cut(s) 526, 652, 843
TseFI GTSAC 2 cut(s) 224, 552
TseI GCWGC 5 cut(s) 127, 371, 593, 674, 736
Tsp45I GTSAC 2 cut(s) 224, 552
TspDTI ATGAA 3 cut(s) 239, 457, 674
TspRI CASTG 3 cut(s) 526, 652, 843
XapI RAATTY 1 cut(s) 230
XceI RCATGY 3 cut(s) 127, 371, 503
XmiI GTMKAC 2 cut(s) 512, 821
XspI CTAG 4 cut(s) 21, 299, 713, 803
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.