Rmu_sc0001516.1_g000040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001516.1
OG0009934
Physical Location & Seq
Forward (+)
153071 .. 156370
3300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001516.1_g000040.1.cds

Sequence Viewer

Length: 729 bp
atggacaaggcccaccgccacgtcagcttccaggacccactgccacgtcagcatcagaccccacagccacatcagcaccagggacccactgacgcgccagcatcaggaccccactgccacgtcagcgacagtcagcaccggtcaaagccggttgaccgattccggtcaacttttcggcgacttccgagaaaatctctggacaccaaatcatcgttctcctccaccactggtgcaccaaatcatcgttctcctctgccaccagcttgggtcgagcgcctgagagcagagagcagagagcctaggtctagcactggaagtcgagcgccaaacgctggagggcttaggacgttgccgaagtcgggggtcattctaattttaaaatattacccctcctcctccactgcttccccaatccctgccttctacagaaccaccgagttccatctccatcaaaccaaatcaaaccagaccctcaaagcagaagttagagaagggcgagctctgaacatgttggggaagaagaggtggttcttgtttaggaggtcgatgaggaaggaatcgtggagggttaggtggaagtacttggggtctgccttcaagtttaggccggtgaatctccagctccccttctacgacgacgttgtgttcaagattgtttcagtctttgaggccatagtgttggagctgagcatcgctttcttctacctttgttgcggttgcagcttctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

27.76

Weight (kDa)

10.62

Isoelectric Point (pI)

64.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 332
AccII CGCG 1 cut(s) 95
AciI CCGC 2 cut(s) 16, 714
AfaI GTAC 1 cut(s) 581
AfiI CCNNNNNNNGG 3 cut(s) 104, 332, 359
AflIII ACRYGT 1 cut(s) 507
AgeI ACCGGT 1 cut(s) 138
AgsI TTSAA 2 cut(s) 598, 649
AjiI CACGTC 3 cut(s) 22, 47, 121
AjnI CCWGG 2 cut(s) 30, 78
AjuI GAANNNNNNNTTGG 4 cut(s) 197, 229, 229, 261
AluBI AGCT 6 cut(s) 27, 263, 500, 622, 685, 723
AluI AGCT 6 cut(s) 27, 263, 500, 622, 685, 723
Alw21I GWGCWC 2 cut(s) 235, 502
Alw44I GTGCAC 1 cut(s) 231
AlwNI CAGNNNCTG 1 cut(s) 726
AoxI GGCC 3 cut(s) 9, 605, 669
ApaLI GTGCAC 1 cut(s) 231
ApeKI GCWGC 1 cut(s) 720
AsiGI ACCGGT 1 cut(s) 138
AspA2I CCTAGG 1 cut(s) 299
AspLEI GCGC 3 cut(s) 97, 276, 325
AspS9I GGNCC 4 cut(s) 10, 34, 83, 107
AsuHPI GGTGA 1 cut(s) 622
AvaII GGWCC 3 cut(s) 34, 83, 107
AvrII CCTAGG 1 cut(s) 299
BaeGI GKGCMC 1 cut(s) 235
BanII GRGCYC 1 cut(s) 502
Bbv12I GWGCWC 2 cut(s) 235, 502
BccI CCATC 2 cut(s) 450, 456
BciT130I CCWGG 2 cut(s) 32, 80
BfaI CTAG 2 cut(s) 300, 306
BfmI CTRYAG 1 cut(s) 424
BfoI RGCGCY 2 cut(s) 277, 326
BisI GCNGC 1 cut(s) 721
BlnI CCTAGG 1 cut(s) 299
BlpI GCTNAGC 1 cut(s) 686
BlsI GCNGC 1 cut(s) 722
BmcAI AGTACT 1 cut(s) 581
Bme1390I CCNGG 2 cut(s) 32, 80
Bme18I GGWCC 3 cut(s) 34, 83, 107
BmgBI CACGTC 3 cut(s) 22, 47, 121
BmgT120I GGNCC 4 cut(s) 10, 34, 83, 107
BmiI GGNNCC 4 cut(s) 36, 84, 85, 109
BmrFI CCNGG 2 cut(s) 32, 80
BmsI GCATC 3 cut(s) 61, 110, 699
BplI GAGNNNNNCTC 2 cut(s) 178, 210
BpmI CTGGAG 2 cut(s) 354, 602
Bpu10I CCTNAGC 1 cut(s) 341
Bpu1102I GCTNAGC 1 cut(s) 686
BsaJI CCNNGG 2 cut(s) 79, 299
BsaWI WCCGGW 2 cut(s) 138, 162
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 332, 359
Bse118I RCCGGY 3 cut(s) 138, 148, 607
Bse1I ACTGG 2 cut(s) 232, 316
BseBI CCWGG 2 cut(s) 32, 80
BseDI CCNNGG 2 cut(s) 79, 299
BseLI CCNNNNNNNGG 3 cut(s) 104, 332, 359
BseMII CTCAG 2 cut(s) 269, 677
BseNI ACTGG 2 cut(s) 232, 316
BseRI GAGGAG 4 cut(s) 208, 240, 382, 385
BseSI GKGCMC 1 cut(s) 235
Bsh1236I CGCG 1 cut(s) 95
BshFI GGCC 3 cut(s) 11, 607, 671
BshTI ACCGGT 1 cut(s) 138
BsiHKAI GWGCWC 2 cut(s) 235, 502
BsiSI CCGG 4 cut(s) 139, 149, 163, 608
BslFI GGGAC 1 cut(s) 96
BslI CCNNNNNNNGG 3 cut(s) 104, 332, 359
BsmFI GGGAC 1 cut(s) 96
BsnI GGCC 3 cut(s) 11, 607, 671
Bsp1286I GDGCHC 2 cut(s) 235, 502
Bsp1720I GCTNAGC 1 cut(s) 686
BspACI CCGC 2 cut(s) 16, 714
BspANI GGCC 3 cut(s) 11, 607, 671
BspCNI CTCAG 2 cut(s) 270, 678
BspFNI CGCG 1 cut(s) 95
BspLI GGNNCC 4 cut(s) 36, 84, 85, 109
BsrFI RCCGGY 3 cut(s) 138, 148, 607
BsrI ACTGG 2 cut(s) 232, 316
BssAI RCCGGY 3 cut(s) 138, 148, 607
BssECI CCNNGG 2 cut(s) 79, 299
BssT1I CCWWGG 1 cut(s) 299
Bst2UI CCWGG 2 cut(s) 32, 80
Bst4CI ACNGT 1 cut(s) 131
Bst6I CTCTTC 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 99, 498
BstDEI CTNAG 3 cut(s) 278, 341, 686
BstFNI CGCG 1 cut(s) 95
BstH2I RGCGCY 2 cut(s) 277, 326
BstHHI GCGC 3 cut(s) 97, 276, 325
BstMWI GCNNNNNNNGC 6 cut(s) 24, 49, 73, 123, 329, 720
BstNI CCWGG 2 cut(s) 32, 80
BstNSI RCATGY 1 cut(s) 511
BstSCI CCNGG 2 cut(s) 30, 78
BstSFI CTRYAG 1 cut(s) 424
BstSLI GKGCMC 1 cut(s) 235
BstUI CGCG 1 cut(s) 95
BstXI CCANNNNNNTGG 2 cut(s) 264, 679
BsuRI GGCC 3 cut(s) 11, 607, 671
BtgZI GCGATG 1 cut(s) 676
BtrI CACGTC 3 cut(s) 22, 47, 121
BtsI GCAGTG 3 cut(s) 38, 112, 399
BtsIMutI CAGTG 6 cut(s) 38, 87, 112, 225, 309, 399
Cac8I GCNNGC 2 cut(s) 99, 498
CaiI CAGNNNCTG 1 cut(s) 726
CfoI GCGC 3 cut(s) 97, 276, 325
Cfr10I RCCGGY 3 cut(s) 138, 148, 607
Cfr13I GGNCC 4 cut(s) 10, 34, 83, 107
CseI GACGC 1 cut(s) 101
Csp6I GTAC 1 cut(s) 580
CspAI ACCGGT 1 cut(s) 138
CviAII CATG 1 cut(s) 508
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 3 cut(s) 278, 341, 686
DraI TTTAAA 1 cut(s) 378
Eam1104I CTCTTC 1 cut(s) 515
EarI CTCTTC 1 cut(s) 515
Ecl136II GAGCTC 1 cut(s) 500
Eco130I CCWWGG 1 cut(s) 299
Eco24I GRGCYC 1 cut(s) 502
Eco47I GGWCC 3 cut(s) 34, 83, 107
Eco53kI GAGCTC 1 cut(s) 500
EcoICRI GAGCTC 1 cut(s) 500
EcoO109I RGGNCCY 3 cut(s) 34, 83, 107
EcoRII CCWGG 2 cut(s) 30, 78
EcoT14I CCWWGG 1 cut(s) 299
EcoT38I GRGCYC 1 cut(s) 502
ErhI CCWWGG 1 cut(s) 299
FaeI CATG 1 cut(s) 511
FaiI YATR 2 cut(s) 509, 674
FaqI GGGAC 1 cut(s) 96
FatI CATG 1 cut(s) 507
Fnu4HI GCNGC 1 cut(s) 721
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 1 cut(s) 721
FspBI CTAG 2 cut(s) 300, 306
GlaI GCGC 3 cut(s) 96, 275, 324
GluI GCNGC 1 cut(s) 721
GsuI CTGGAG 2 cut(s) 354, 602
HaeII RGCGCY 2 cut(s) 277, 326
HaeIII GGCC 3 cut(s) 11, 607, 671
HapII CCGG 4 cut(s) 139, 149, 163, 608
HgaI GACGC 1 cut(s) 101
HhaI GCGC 3 cut(s) 97, 276, 325
Hin1II CATG 1 cut(s) 511
Hin6I GCGC 3 cut(s) 95, 274, 323
HinP1I GCGC 3 cut(s) 95, 274, 323
HincII GTYRAC 2 cut(s) 154, 168
HindII GTYRAC 2 cut(s) 154, 168
HinfI GANTC 3 cut(s) 159, 557, 613
HpaII CCGG 4 cut(s) 139, 149, 163, 608
HphI GGTGA 1 cut(s) 622
Hpy166II GTNNAC 3 cut(s) 154, 168, 233
Hpy188I TCNGA 4 cut(s) 57, 186, 504, 728
Hpy188III TCNNGA 3 cut(s) 105, 197, 649
Hpy8I GTNNAC 3 cut(s) 154, 168, 233
Hpy99I CGWCG 2 cut(s) 638, 641
HpyAV CCTTC 5 cut(s) 430, 485, 547, 604, 637
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4IV ACGT 5 cut(s) 21, 46, 120, 347, 639
HpyCH4V TGCA 2 cut(s) 233, 720
HpyF10VI GCNNNNNNNGC 6 cut(s) 24, 49, 73, 123, 329, 720
HpyF3I CTNAG 3 cut(s) 278, 341, 686
HpySE526I ACGT 5 cut(s) 21, 46, 120, 347, 639
Hsp92II CATG 1 cut(s) 511
HspAI GCGC 3 cut(s) 95, 274, 323
KflI GGGWCCC 1 cut(s) 83
LmnI GCTCC 2 cut(s) 627, 682
LweI GCATC 3 cut(s) 61, 110, 699
MaeI CTAG 2 cut(s) 300, 306
MaeII ACGT 5 cut(s) 21, 46, 120, 347, 639
MboII GAAGA 3 cut(s) 529, 532, 691
MhlI GDGCHC 2 cut(s) 235, 502
MluCI AATT 1 cut(s) 372
MmeI TCCRAC 1 cut(s) 660
MseI TTAA 1 cut(s) 377
MspI CCGG 4 cut(s) 139, 149, 163, 608
MspR9I CCNGG 2 cut(s) 32, 80
MvaI CCWGG 2 cut(s) 32, 80
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 6 cut(s) 24, 49, 73, 123, 329, 720
NlaIII CATG 1 cut(s) 511
NlaIV GGNNCC 4 cut(s) 36, 84, 85, 109
NspI RCATGY 1 cut(s) 511
PciI ACATGT 1 cut(s) 507
PfeI GAWTC 3 cut(s) 159, 557, 613
PflMI CCANNNNNTGG 1 cut(s) 332
PfoI TCCNGGA 1 cut(s) 30
PinAI ACCGGT 1 cut(s) 138
PkrI GCNGC 1 cut(s) 722
PpuMI RGGWCCY 3 cut(s) 34, 83, 107
PscI ACATGT 1 cut(s) 507
Psp124BI GAGCTC 1 cut(s) 502
Psp5II RGGWCCY 3 cut(s) 34, 83, 107
Psp6I CCWGG 2 cut(s) 30, 78
PspGI CCWGG 2 cut(s) 30, 78
PspN4I GGNNCC 4 cut(s) 36, 84, 85, 109
PspPI GGNCC 4 cut(s) 10, 34, 83, 107
PspPPI RGGWCCY 3 cut(s) 34, 83, 107
PstNI CAGNNNCTG 1 cut(s) 726
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
SacI GAGCTC 1 cut(s) 502
SaqAI TTAA 1 cut(s) 377
SatI GCNGC 1 cut(s) 721
Sau96I GGNCC 4 cut(s) 10, 34, 83, 107
ScaI AGTACT 1 cut(s) 581
ScrFI CCNGG 2 cut(s) 32, 80
SduI GDGCHC 2 cut(s) 235, 502
SfaNI GCATC 3 cut(s) 61, 110, 699
SfcI CTRYAG 1 cut(s) 424
SinI GGWCC 3 cut(s) 34, 83, 107
Sse9I AATT 1 cut(s) 372
SsiI CCGC 2 cut(s) 16, 714
SspI AATATT 1 cut(s) 383
SspMI CTAG 2 cut(s) 300, 306
SstI GAGCTC 1 cut(s) 502
StyD4I CCNGG 2 cut(s) 30, 78
StyI CCWWGG 1 cut(s) 299
TaaI ACNGT 1 cut(s) 131
TaiI ACGT 5 cut(s) 24, 49, 123, 350, 642
TaqI TCGA 3 cut(s) 270, 319, 545
TaqII GACCGA 1 cut(s) 171
TasI AATT 1 cut(s) 372
TatI WGTACW 1 cut(s) 579
TfiI GAWTC 3 cut(s) 159, 557, 613
Tru1I TTAA 1 cut(s) 377
Tru9I TTAA 1 cut(s) 377
TscAI CASTG 6 cut(s) 45, 94, 119, 232, 316, 406
TseI GCWGC 1 cut(s) 720
TspRI CASTG 6 cut(s) 45, 94, 119, 232, 316, 406
Van91I CCANNNNNTGG 1 cut(s) 332
VneI GTGCAC 1 cut(s) 231
VpaK11BI GGWCC 3 cut(s) 34, 83, 107
XceI RCATGY 1 cut(s) 511
XmaJI CCTAGG 1 cut(s) 299
XspI CTAG 2 cut(s) 300, 306
ZrmI AGTACT 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.