Rorug07G0069200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0009934
Physical Location & Seq
Reverse (-)
5247390 .. 5247959
570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0069200.1

Sequence Viewer

Length: 570 bp
ATGGCATTGCAACTTGTTGCAAAATTTTCAGGAAAAAATTATATTCGAATCATAGTCATGGAAAACGGTATAGAGAGCAGTTGTACATATATTGTTACAGAAGATCATGAGTCCAGAGTCATTGTTGAAGGATTGGAGAATACCATGACAGCAAAGCTTCGCAAGAACTCACTCTGGTCTGGTAAGAGCTGCATTTTCAGAGTTCCCGAAGTGCTTCGGAGGCATAAACCAGAGGCATATGTACCTGATGTTGTGTCAATCGGTCCCTTTCACCGAGGCGGTGAAAAATTTCAACCCATAGAAGATGTGAAACAATGGTACTTACATAACCTTCTCTCTCGCATGAATATAAGCTTGAAAACCTTAATGGAAGGCATTGATAATATTATTGAGTTTGAGAAACGCGCCAGTGAATTTTATTCAGAACCCCCTGATCTTAGCCGGGATGATTTCATAGAAATGCTGATACTTGATGGTTGCTTCATGGTAGAACTATTTTGGAAGTATTTCTGTCGGTCGTGGATGCGTTTATGTAAGAAAGATCTAGTTCCAGCTGAAGTACTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

22.09

Weight (kDa)

6.12

Isoelectric Point (pI)

63.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 65 - 170 1.9e-29 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 405
AciI CCGC 1 cut(s) 279
AcsI RAATTY 3 cut(s) 23, 287, 413
AfaI GTAC 4 cut(s) 85, 243, 320, 561
AgsI TTSAA 3 cut(s) 128, 293, 358
AluBI AGCT 4 cut(s) 157, 189, 354, 554
AluI AGCT 4 cut(s) 157, 189, 354, 554
ApeKI GCWGC 1 cut(s) 189
ApoI RAATTY 3 cut(s) 23, 287, 413
Asp700I GAANNNNTTC 3 cut(s) 213, 288, 506
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 1 cut(s) 263
AsuC2I CCSGG 1 cut(s) 443
AsuHPI GGTGA 2 cut(s) 263, 293
AsuII TTCGAA 1 cut(s) 46
AvaII GGWCC 1 cut(s) 263
BbvI GCAGC 1 cut(s) 176
BccI CCATC 1 cut(s) 467
BcnI CCSGG 1 cut(s) 443
BfaI CTAG 1 cut(s) 545
BglII AGATCT 1 cut(s) 541
BisI GCNGC 1 cut(s) 190
BlsI GCNGC 1 cut(s) 191
BmcAI AGTACT 1 cut(s) 561
Bme1390I CCNGG 1 cut(s) 443
Bme18I GGWCC 1 cut(s) 263
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 1 cut(s) 265
BmrFI CCNGG 1 cut(s) 443
BmsI GCATC 1 cut(s) 513
Bpu14I TTCGAA 1 cut(s) 46
BpuMI CCSGG 1 cut(s) 443
BsaJI CCNNGG 1 cut(s) 274
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 1 cut(s) 5
BseDI CCNNGG 1 cut(s) 274
BseGI GGATG 2 cut(s) 451, 528
BseMI GCAATG 1 cut(s) 5
BseNI ACTGG 1 cut(s) 408
BseXI GCAGC 1 cut(s) 176
Bsh1236I CGCG 1 cut(s) 405
Bsh1285I CGRYCG 1 cut(s) 518
BsiEI CGRYCG 1 cut(s) 518
BsiSI CCGG 1 cut(s) 442
BslFI GGGAC 1 cut(s) 249
BsmFI GGGAC 1 cut(s) 249
Bsp119I TTCGAA 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 83
Bsp143I GATC 3 cut(s) 103, 433, 541
BspACI CCGC 1 cut(s) 279
BspFNI CGCG 1 cut(s) 405
BspHI TCATGA 1 cut(s) 106
BspLI GGNNCC 1 cut(s) 265
BspT104I TTCGAA 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 5
BsrGI TGTACA 1 cut(s) 83
BsrI ACTGG 1 cut(s) 408
BssECI CCNNGG 1 cut(s) 274
BssMI GATC 3 cut(s) 103, 433, 541
Bst4CI ACNGT 1 cut(s) 68
BstAUI TGTACA 1 cut(s) 83
BstBI TTCGAA 1 cut(s) 46
BstDEI CTNAG 1 cut(s) 437
BstF5I GGATG 2 cut(s) 451, 528
BstFNI CGCG 1 cut(s) 405
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 3 cut(s) 106, 436, 544
BstMBI GATC 3 cut(s) 103, 433, 541
BstMCI CGRYCG 1 cut(s) 518
BstMWI GCNNNNNNNGC 1 cut(s) 220
BstSCI CCNGG 1 cut(s) 441
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 1 cut(s) 176
BstX2I RGATCY 1 cut(s) 541
BstYI RGATCY 1 cut(s) 541
BtsCI GGATG 2 cut(s) 451, 528
BtsIMutI CAGTG 1 cut(s) 415
CciI TCATGA 1 cut(s) 106
CfoI GCGC 1 cut(s) 407
Cfr13I GGNCC 1 cut(s) 263
Csp6I GTAC 4 cut(s) 84, 242, 319, 560
CviAII CATG 5 cut(s) 58, 107, 145, 343, 484
CviJI RGCY 5 cut(s) 157, 189, 354, 441, 554
CviKI_1 RGCY 5 cut(s) 157, 189, 354, 441, 554
CviQI GTAC 4 cut(s) 84, 242, 319, 560
DdeI CTNAG 1 cut(s) 437
DpnI GATC 3 cut(s) 105, 435, 543
DpnII GATC 3 cut(s) 103, 433, 541
Eco47I GGWCC 1 cut(s) 263
FaeI CATG 5 cut(s) 61, 110, 148, 346, 487
FaqI GGGAC 1 cut(s) 249
FatI CATG 5 cut(s) 57, 106, 144, 342, 483
FauNDI CATATG 1 cut(s) 238
Fnu4HI GCNGC 1 cut(s) 190
FokI GGATG 2 cut(s) 458, 535
Fsp4HI GCNGC 1 cut(s) 190
FspBI CTAG 1 cut(s) 545
GlaI GCGC 1 cut(s) 406
GluI GCNGC 1 cut(s) 190
HapII CCGG 1 cut(s) 442
HhaI GCGC 1 cut(s) 407
Hin1II CATG 5 cut(s) 61, 110, 148, 346, 487
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HindIII AAGCTT 2 cut(s) 155, 352
HinfI GANTC 3 cut(s) 48, 110, 117
HpaII CCGG 1 cut(s) 442
HphI GGTGA 2 cut(s) 263, 293
Hpy188I TCNGA 3 cut(s) 200, 219, 424
Hpy188III TCNNGA 4 cut(s) 30, 107, 114, 206
HpyAV CCTTC 3 cut(s) 122, 341, 365
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4V TGCA 3 cut(s) 10, 20, 192
HpyF10VI GCNNNNNNNGC 1 cut(s) 220
HpyF3I CTNAG 1 cut(s) 437
Hsp92II CATG 5 cut(s) 61, 110, 148, 346, 487
HspAI GCGC 1 cut(s) 405
Kzo9I GATC 3 cut(s) 103, 433, 541
Lsp1109I GCAGC 1 cut(s) 176
LweI GCATC 1 cut(s) 513
MaeI CTAG 1 cut(s) 545
MaeIII GTNAC 1 cut(s) 94
MalI GATC 3 cut(s) 105, 435, 543
MboI GATC 3 cut(s) 103, 433, 541
MboII GAAGA 2 cut(s) 113, 314
MflI RGATCY 1 cut(s) 541
MluCI AATT 5 cut(s) 23, 37, 287, 413, 565
MlyI GAGTC 2 cut(s) 119, 126
MnlI CCTC 3 cut(s) 213, 226, 269
MroXI GAANNNNTTC 3 cut(s) 213, 288, 506
MseI TTAA 1 cut(s) 365
MslI CAYNNNNRTG 2 cut(s) 56, 458
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 1 cut(s) 442
MspR9I CCNGG 1 cut(s) 443
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 1 cut(s) 220
NciI CCSGG 1 cut(s) 443
NdeI CATATG 1 cut(s) 238
NdeII GATC 3 cut(s) 103, 433, 541
NlaIII CATG 5 cut(s) 61, 110, 148, 346, 487
NlaIV GGNNCC 1 cut(s) 265
NspV TTCGAA 1 cut(s) 46
PagI TCATGA 1 cut(s) 106
PdmI GAANNNNTTC 3 cut(s) 213, 288, 506
PfeI GAWTC 1 cut(s) 48
PkrI GCNGC 1 cut(s) 191
PleI GAGTC 2 cut(s) 118, 125
PpsI GAGTC 2 cut(s) 118, 125
PspN4I GGNNCC 1 cut(s) 265
PspPI GGNCC 1 cut(s) 263
PsuI RGATCY 1 cut(s) 541
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 4 cut(s) 85, 243, 320, 561
RsaNI GTAC 4 cut(s) 84, 242, 319, 560
RseI CAYNNNNRTG 2 cut(s) 56, 458
SaqAI TTAA 1 cut(s) 365
SatI GCNGC 1 cut(s) 190
Sau3AI GATC 3 cut(s) 103, 433, 541
Sau96I GGNCC 1 cut(s) 263
ScaI AGTACT 1 cut(s) 561
SchI GAGTC 2 cut(s) 119, 126
ScrFI CCNGG 1 cut(s) 443
SetI ASST 7 cut(s) 159, 191, 247, 333, 356, 365, 556
SfaNI GCATC 1 cut(s) 513
SfuI TTCGAA 1 cut(s) 46
SinI GGWCC 1 cut(s) 263
SmiMI CAYNNNNRTG 2 cut(s) 56, 458
Sse9I AATT 5 cut(s) 23, 37, 287, 413, 565
SsiI CCGC 1 cut(s) 279
SspI AATATT 1 cut(s) 385
SspMI CTAG 1 cut(s) 545
StyD4I CCNGG 1 cut(s) 441
TaaI ACNGT 1 cut(s) 68
TaqI TCGA 1 cut(s) 46
TaqII GACCGA 2 cut(s) 251, 504
TasI AATT 5 cut(s) 23, 37, 287, 413, 565
TatI WGTACW 2 cut(s) 83, 559
TfiI GAWTC 1 cut(s) 48
Tru1I TTAA 1 cut(s) 365
Tru9I TTAA 1 cut(s) 365
TscAI CASTG 1 cut(s) 415
TseI GCWGC 1 cut(s) 189
TspDTI ATGAA 3 cut(s) 359, 442, 472
TspRI CASTG 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 263
XapI RAATTY 3 cut(s) 23, 287, 413
XmnI GAANNNNTTC 3 cut(s) 213, 288, 506
XspI CTAG 1 cut(s) 545
ZrmI AGTACT 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.