Rmu_sc0001640.1_g000015

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001640.1
OG0004981
Physical Location & Seq
Reverse (-)
70111 .. 74355
4245 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 900 bp
atggcaacttttgtagaaaaaatttgtttcatcattttgatcctcattgcattccaatctgtttcggcaacgaaacattcaatcatagagtcgaaaccatgcaagaggctcgtgctctattaccacgacatccttttcaatggcactgatgtggcaaatgctacatctgctagggcagcaaatgggacgggcctcggcaactaccagtttggtatgctggttgtcttcaatgatcctctgaccaaagaccaccatcttctgtctcctgccgtcggaagagctcaaggcttctttttctatgacatgaaggacgactacactgcctggtttgcctacactttggtgttcaactcgagcgagcacaagggtacactcaatatcatgggggcggatttgatgtatgccgagactagggatctttcggtggttggaggaacgggagatttcttcatggctagagggatttgcacattcaagactgatacttttcagggtgccaactattttcgtctccaaatggacatcaagttgtatgaatgtcttgagcttaaagcgggaggtggtggcatcatcgctgatggtgacatcaacattggtggcggcggctgcgactgcgacgtgggtggtagttgcatcgacgctggtggcggcgacagtgcagtggctgcagtggccaatctaacagttttagattggtctaggcctgtagttgagcctgggcttcaagttttgctagggaaggcttcgcctagggatatctcgcttgtctggcttgcacattttgcagttgatcctcatgccacttgtaccaaggcccaatggacgttgtggaagtctcctatagcccgcaaggccctcaaggacctggtcctcatgttggtcgcgacttctattgcttaa

Protein Analysis

299

Amino Acids

32.26

Weight (kDa)

5.77

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 34 - 176 1.2e-36 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 494
AccII CGCG 1 cut(s) 884
AciI CCGC 6 cut(s) 389, 554, 600, 603, 648, 847
AclWI GGATC 4 cut(s) 34, 227, 423, 785
AcoI YGGCCR 1 cut(s) 672
AcsI RAATTY 1 cut(s) 21
AfaI GTAC 2 cut(s) 370, 808
AfiI CCNNNNNNNGG 3 cut(s) 272, 411, 877
AgsI TTSAA 6 cut(s) 81, 139, 229, 349, 475, 725
AjiI CACGTC 1 cut(s) 619
AjnI CCWGG 3 cut(s) 323, 715, 864
AleI CACNNNNGTG 2 cut(s) 149, 341
AluBI AGCT 2 cut(s) 281, 547
AluI AGCT 2 cut(s) 281, 547
Alw21I GWGCWC 3 cut(s) 117, 283, 363
Alw26I GTCTC 4 cut(s) 267, 401, 515, 840
AlwI GGATC 4 cut(s) 34, 227, 423, 785
AlwNI CAGNNNCTG 1 cut(s) 665
Ama87I CYCGRG 1 cut(s) 352
AoxI GGCC 5 cut(s) 190, 672, 701, 813, 852
ApeKI GCWGC 3 cut(s) 176, 606, 665
ApoI RAATTY 1 cut(s) 21
AspA2I CCTAGG 1 cut(s) 749
AspS9I GGNCC 5 cut(s) 190, 814, 853, 862, 868
AsuHPI GGTGA 1 cut(s) 593
AvaI CYCGRG 1 cut(s) 352
AvaII GGWCC 2 cut(s) 862, 868
AvrII CCTAGG 1 cut(s) 749
BalI TGGCCA 1 cut(s) 674
BanI GGYRCC 1 cut(s) 494
BanII GRGCYC 1 cut(s) 283
BarI GAAGNNNNNNTAC 2 cut(s) 299, 331
BauI CACGAG 1 cut(s) 110
BbsI GAAGAC 1 cut(s) 217
Bbv12I GWGCWC 3 cut(s) 117, 283, 363
BbvI GCAGC 3 cut(s) 188, 593, 652
BccI CCATC 2 cut(s) 261, 572
BceAI ACGGC 1 cut(s) 254
BcgI CGANNNNNNTGC 4 cut(s) 91, 125, 302, 336
BciT130I CCWGG 3 cut(s) 325, 717, 866
BcoDI GTCTC 4 cut(s) 267, 401, 515, 840
BfaI CTAG 6 cut(s) 171, 411, 456, 699, 734, 750
BfmI CTRYAG 3 cut(s) 666, 705, 840
BglI GCCNNNNNGGC 1 cut(s) 851
BisI GCNGC 6 cut(s) 177, 601, 604, 607, 649, 666
BlnI CCTAGG 1 cut(s) 749
BlsI GCNGC 6 cut(s) 178, 602, 605, 608, 650, 667
Bme1390I CCNGG 3 cut(s) 325, 717, 866
Bme18I GGWCC 2 cut(s) 862, 868
BmeT110I CYCGRG 1 cut(s) 352
BmgBI CACGTC 1 cut(s) 619
BmgT120I GGNCC 5 cut(s) 190, 814, 853, 862, 868
BmiI GGNNCC 1 cut(s) 496
BmrFI CCNGG 3 cut(s) 325, 717, 866
BmsI GCATC 2 cut(s) 576, 642
BpiI GAAGAC 1 cut(s) 217
BpuEI CTTGAG 3 cut(s) 267, 563, 842
BsaJI CCNNGG 4 cut(s) 193, 716, 749, 810
Bsc4I CCNNNNNNNGG 3 cut(s) 272, 411, 877
Bse1I ACTGG 1 cut(s) 205
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 3 cut(s) 325, 717, 866
BseDI CCNNGG 4 cut(s) 193, 716, 749, 810
BseGI GGATG 1 cut(s) 129
BseLI CCNNNNNNNGG 3 cut(s) 272, 411, 877
BseMI GCAATG 1 cut(s) 45
BseNI ACTGG 1 cut(s) 205
BseXI GCAGC 3 cut(s) 188, 593, 652
BsgI GTGCAG 1 cut(s) 678
Bsh1236I CGCG 1 cut(s) 884
BshFI GGCC 5 cut(s) 192, 674, 703, 815, 854
BshNI GGYRCC 1 cut(s) 494
BsiHKAI GWGCWC 3 cut(s) 117, 283, 363
BsiHKCI CYCGRG 1 cut(s) 352
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 3 cut(s) 272, 411, 877
BsmAI GTCTC 4 cut(s) 267, 401, 515, 840
BsmBI CGTCTC 1 cut(s) 515
BsmFI GGGAC 1 cut(s) 199
BsmI GAATGC 1 cut(s) 50
BsnI GGCC 5 cut(s) 192, 674, 703, 815, 854
BsoBI CYCGRG 1 cut(s) 352
Bsp1286I GDGCHC 3 cut(s) 117, 283, 363
Bsp143I GATC 4 cut(s) 39, 232, 415, 790
Bsp68I TCGCGA 1 cut(s) 884
BspACI CCGC 6 cut(s) 389, 554, 600, 603, 648, 847
BspANI GGCC 5 cut(s) 192, 674, 703, 815, 854
BspFNI CGCG 1 cut(s) 884
BspLI GGNNCC 1 cut(s) 496
BspMAI CTGCAG 1 cut(s) 670
BspPI GGATC 4 cut(s) 34, 227, 423, 785
BspQI GCTCTTC 1 cut(s) 271
BspT107I GGYRCC 1 cut(s) 494
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 1 cut(s) 205
BssECI CCNNGG 4 cut(s) 193, 716, 749, 810
BssMI GATC 4 cut(s) 39, 232, 415, 790
BssSI CACGAG 1 cut(s) 110
BssT1I CCWWGG 2 cut(s) 749, 810
Bst2BI CACGAG 1 cut(s) 110
Bst2UI CCWGG 3 cut(s) 325, 717, 866
Bst4CI ACNGT 2 cut(s) 656, 685
Bst6I CTCTTC 1 cut(s) 271
BstAPI GCANNNNNTGC 2 cut(s) 665, 782
BstC8I GCNNGC 3 cut(s) 359, 774, 847
BstF5I GGATG 1 cut(s) 129
BstFNI CGCG 1 cut(s) 884
BstKTI GATC 4 cut(s) 42, 235, 418, 793
BstMAI GTCTC 4 cut(s) 267, 401, 515, 840
BstMBI GATC 4 cut(s) 39, 232, 415, 790
BstNI CCWGG 3 cut(s) 325, 717, 866
BstSCI CCNGG 3 cut(s) 323, 715, 864
BstSFI CTRYAG 3 cut(s) 666, 705, 840
BstUI CGCG 1 cut(s) 884
BstV1I GCAGC 3 cut(s) 188, 593, 652
BstV2I GAAGAC 1 cut(s) 217
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 5 cut(s) 192, 674, 703, 815, 854
BtgZI GCGATG 1 cut(s) 556
BtrI CACGTC 1 cut(s) 619
BtsCI GGATG 1 cut(s) 129
BtsI GCAGTG 3 cut(s) 318, 666, 675
BtsIMutI CAGTG 5 cut(s) 144, 318, 661, 666, 675
BtuMI TCGCGA 1 cut(s) 884
Cac8I GCNNGC 3 cut(s) 359, 774, 847
CaiI CAGNNNCTG 1 cut(s) 665
Cfr13I GGNCC 5 cut(s) 190, 814, 853, 862, 868
CseI GACGC 1 cut(s) 647
CsiI ACCWGGT 1 cut(s) 864
Csp6I GTAC 2 cut(s) 369, 807
CspCI CAANNNNNGTGG 2 cut(s) 577, 612
CviAII CATG 6 cut(s) 99, 304, 382, 451, 797, 874
CviQI GTAC 2 cut(s) 369, 807
DpnI GATC 4 cut(s) 41, 234, 417, 792
DpnII GATC 4 cut(s) 39, 232, 415, 790
EaeI YGGCCR 1 cut(s) 672
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
EciI GGCGGA 1 cut(s) 404
Ecl136II GAGCTC 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 749, 810
Eco147I AGGCCT 1 cut(s) 703
Eco24I GRGCYC 1 cut(s) 283
Eco32I GATATC 1 cut(s) 757
Eco47I GGWCC 2 cut(s) 862, 868
Eco53kI GAGCTC 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 352
EcoICRI GAGCTC 1 cut(s) 281
EcoO109I RGGNCCY 2 cut(s) 853, 862
EcoRII CCWGG 3 cut(s) 323, 715, 864
EcoRV GATATC 1 cut(s) 757
EcoT14I CCWWGG 2 cut(s) 749, 810
EcoT38I GRGCYC 1 cut(s) 283
ErhI CCWWGG 2 cut(s) 749, 810
Esp3I CGTCTC 1 cut(s) 515
FaeI CATG 6 cut(s) 102, 307, 385, 454, 800, 877
FaqI GGGAC 1 cut(s) 199
FatI CATG 6 cut(s) 98, 303, 381, 450, 796, 873
FauI CCCGC 2 cut(s) 547, 854
Fnu4HI GCNGC 6 cut(s) 177, 601, 604, 607, 649, 666
FokI GGATG 1 cut(s) 116
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 6 cut(s) 177, 601, 604, 607, 649, 666
FspBI CTAG 6 cut(s) 171, 411, 456, 699, 734, 750
GluI GCNGC 6 cut(s) 177, 601, 604, 607, 649, 666
HaeIII GGCC 5 cut(s) 192, 674, 703, 815, 854
HgaI GACGC 1 cut(s) 647
Hin1II CATG 6 cut(s) 102, 307, 385, 454, 800, 877
HinfI GANTC 1 cut(s) 89
HphI GGTGA 1 cut(s) 593
Hpy166II GTNNAC 1 cut(s) 371
Hpy188I TCNGA 2 cut(s) 240, 275
Hpy188III TCNNGA 3 cut(s) 475, 542, 883
Hpy8I GTNNAC 1 cut(s) 371
Hpy99I CGWCG 3 cut(s) 275, 620, 641
HpyAV CCTTC 2 cut(s) 301, 733
HpyCH4III ACNGT 2 cut(s) 656, 685
HpyCH4IV ACGT 2 cut(s) 618, 824
HpyCH4V TGCA 8 cut(s) 50, 102, 468, 633, 659, 668, 776, 785
HpySE526I ACGT 2 cut(s) 618, 824
Hsp92II CATG 6 cut(s) 102, 307, 385, 454, 800, 877
Kzo9I GATC 4 cut(s) 39, 232, 415, 790
LguI GCTCTTC 1 cut(s) 271
Lsp1109I GCAGC 3 cut(s) 188, 593, 652
LweI GCATC 2 cut(s) 576, 642
MabI ACCWGGT 1 cut(s) 864
MaeI CTAG 6 cut(s) 171, 411, 456, 699, 734, 750
MaeII ACGT 2 cut(s) 618, 824
MaeIII GTNAC 1 cut(s) 581
MalI GATC 4 cut(s) 41, 234, 417, 792
MboI GATC 4 cut(s) 39, 232, 415, 790
MboII GAAGA 4 cut(s) 217, 248, 288, 439
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 3 cut(s) 117, 283, 363
MlsI TGGCCA 1 cut(s) 674
MluCI AATT 1 cut(s) 21
MluNI TGGCCA 1 cut(s) 674
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 2 cut(s) 253, 409
Mox20I TGGCCA 1 cut(s) 674
MscI TGGCCA 1 cut(s) 674
MseI TTAA 2 cut(s) 549, 898
MslI CAYNNNNRTG 2 cut(s) 149, 341
Msp20I TGGCCA 1 cut(s) 674
MspR9I CCNGG 3 cut(s) 325, 717, 866
Mva1269I GAATGC 1 cut(s) 50
MvaI CCWGG 3 cut(s) 325, 717, 866
MvnI CGCG 1 cut(s) 884
NdeII GATC 4 cut(s) 39, 232, 415, 790
NlaIII CATG 6 cut(s) 102, 307, 385, 454, 800, 877
NlaIV GGNNCC 1 cut(s) 496
NmeAIII GCCGAG 2 cut(s) 174, 430
NmuCI GTSAC 1 cut(s) 581
NruI TCGCGA 1 cut(s) 884
OliI CACNNNNGTG 2 cut(s) 149, 341
PaeR7I CTCGAG 1 cut(s) 352
PceI AGGCCT 1 cut(s) 703
PciSI GCTCTTC 1 cut(s) 271
PctI GAATGC 1 cut(s) 50
PflFI GACNNNGTC 1 cut(s) 866
PkrI GCNGC 6 cut(s) 178, 602, 605, 608, 650, 667
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
PpuMI RGGWCCY 1 cut(s) 862
Psp124BI GAGCTC 1 cut(s) 283
Psp5II RGGWCCY 1 cut(s) 862
Psp6I CCWGG 3 cut(s) 323, 715, 864
PspGI CCWGG 3 cut(s) 323, 715, 864
PspN4I GGNNCC 1 cut(s) 496
PspPI GGNCC 5 cut(s) 190, 814, 853, 862, 868
PspPPI RGGWCCY 1 cut(s) 862
PspXI VCTCGAGB 1 cut(s) 352
PstI CTGCAG 1 cut(s) 670
PstNI CAGNNNCTG 1 cut(s) 665
PsuI RGATCY 1 cut(s) 415
PsyI GACNNNGTC 1 cut(s) 866
RruI TCGCGA 1 cut(s) 884
RsaI GTAC 2 cut(s) 370, 808
RsaNI GTAC 2 cut(s) 369, 807
RseI CAYNNNNRTG 2 cut(s) 149, 341
SacI GAGCTC 1 cut(s) 283
SapI GCTCTTC 1 cut(s) 271
SaqAI TTAA 2 cut(s) 549, 898
SatI GCNGC 6 cut(s) 177, 601, 604, 607, 649, 666
Sau3AI GATC 4 cut(s) 39, 232, 415, 790
Sau96I GGNCC 5 cut(s) 190, 814, 853, 862, 868
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 3 cut(s) 325, 717, 866
SduI GDGCHC 3 cut(s) 117, 283, 363
SetI ASST 6 cut(s) 283, 549, 562, 621, 827, 867
SexAI ACCWGGT 1 cut(s) 864
SfaNI GCATC 2 cut(s) 576, 642
SfcI CTRYAG 3 cut(s) 666, 705, 840
Sfr274I CTCGAG 1 cut(s) 352
SinI GGWCC 2 cut(s) 862, 868
SlaI CTCGAG 1 cut(s) 352
SmiMI CAYNNNNRTG 2 cut(s) 149, 341
SmlI CTYRAG 4 cut(s) 282, 352, 542, 857
SmoI CTYRAG 4 cut(s) 282, 352, 542, 857
Sse9I AATT 1 cut(s) 21
SseBI AGGCCT 1 cut(s) 703
SsiI CCGC 6 cut(s) 389, 554, 600, 603, 648, 847
SspMI CTAG 6 cut(s) 171, 411, 456, 699, 734, 750
SstI GAGCTC 1 cut(s) 283
StuI AGGCCT 1 cut(s) 703
StyD4I CCNGG 3 cut(s) 323, 715, 864
StyI CCWWGG 2 cut(s) 749, 810
TaaI ACNGT 2 cut(s) 656, 685
TaiI ACGT 2 cut(s) 621, 827
TaqI TCGA 3 cut(s) 92, 353, 636
TasI AATT 1 cut(s) 21
TauI GCSGC 3 cut(s) 603, 606, 651
Tru1I TTAA 2 cut(s) 549, 898
Tru9I TTAA 2 cut(s) 549, 898
TscAI CASTG 5 cut(s) 151, 325, 661, 666, 675
TseFI GTSAC 1 cut(s) 581
TseI GCWGC 3 cut(s) 176, 606, 665
Tsp45I GTSAC 1 cut(s) 581
TspDTI ATGAA 4 cut(s) 19, 320, 439, 549
TspRI CASTG 5 cut(s) 151, 325, 661, 666, 675
Tth111I GACNNNGTC 1 cut(s) 866
VpaK11BI GGWCC 2 cut(s) 862, 868
XapI RAATTY 1 cut(s) 21
XhoI CTCGAG 1 cut(s) 352
XmaJI CCTAGG 1 cut(s) 749
XspI CTAG 6 cut(s) 171, 411, 456, 699, 734, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.