Rmu_sc0001803.1_g000037

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001803.1
OG0015588
Physical Location & Seq
Forward (+)
143297 .. 147806
4510 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3852 bp
atgtgtcgtataataatagctcctatagtctccggtgaaagtctcctctcaaataccacacatcatagacgtccagtagaatccaggcctctctcgcattcgacagtcccgacggctccagattcgacggaattgaccagcggcgacactgcattggtccttctctctctcctcggttctggagcatctaatgcaatgtcagaaatggataggtcattgaagcgtggttcaagtgatattggatgcggttttgaagaggcaaaagctaagaagaaacaaaaagctagagacgaagaggaagtgatggaagaagtcggagttattgtacaagaagaagcagaaattgaagggcaaaaaaaaaagcagcaagattccccagtgattcagctgatggaatcatcgtcagctctcatggttgaaaaccctgagccaaaatctggggataatcaggaatcagtggacctggatccctctacatctcttcgttcagcagatccttcgaagaagtatgatgtttttctgagtttcaggggtgcagacactcgtctgggtattgtatcccacttatacgatgaattgagagatgggagagggcttcgaacgttcatggatgacaaaaagcttgaactaggggatcatatttctcaaagtctgctaaaggcaattgaagaatccagttttgcaattgttgttttgtcaaagaactatgctgaatcaccatggtgtttggaggaacttacaaagatttgccaatgcatgaaagacaataacagaattataccggttttttatcatgtcgaacctactgatgtacggcatcagaagggtagttttgaaaatgctttcaaaaatcatgtaaacaatggacggcacaattcaaagaagctggagcggtggaaagaagctttaaaaacagttgccgatttctctggatataatgcaaaggataagactgataaagaagctgtcaaagccattgtggaatctctgtgcaagaaagtatggcctactgaaattgagtttattatttcctccagatattttgaagaatttgaagcaacaagacaagccatggacgatgtagtgaaggcgctaaaagatgaaaatgtcactgccgttggggtctacggcatgggaggcgtcggaaaaacaaccatggtggaacatgtcgctgtagaagcccaaaaaaaggggcttttccgtcaggtgattatggctaacatatcccaaaagcccaacttgtgggaaaagcccgacttgtgggaaaagctcaacttgtgggatattcaggaaaagcccaccttgttggatattcagagtggattggcacacatgttgggtttcaaatttgaagttgacggagaatttgcaagagcccgcgaattgaggaagtggataaagaagggagagaggattcttattatcttggacaacatttggaatgcaatagagttgacagacataggaattcccagttacgatgagcttaaaagacgcaattccaaggttctactcaccactaggagattgcaagtttgcggtgatatgaaatgtgacaaaaacattcctctcaataccttagctaaaaatgattcttggaatttgtttgtgaagatagcagaaacaccaaccaatttctatggcattgcaagtgaggtagcaggagaatgtgctggtcttccagttgcattgatagcagttgcaaaggcacttcggggtaaaaacttcggccaatggaaggaagctgctaggcgactaaaggcgtctaaaactacttcctgccctgaagatgaaagacgtgtatttaaatgcataaagtttagctatgattacttgatatctgatgactccaaattatgcttcttgctttgctgcctattcccagaggattatgacatccaaattgaagacttgctgaagtattggattggggaaggattgtttcaagagaacaaaatacaagacgccagagcggcaatatgttctgtggtccagtcccttaaagattctagcctacttttggatggtaaaagggacagaggatgcataaggatgcatgatgtcatcagagatgttgccatatcaatttccttatctatggatgggcaccgacggttttttgtaaaagctggttgtgagttagggagttggccaggaattgatgcacatgggcgctacaatgcaatctcactaatgaagaacaaaatccgcatgcttcccgatgacttggtatgttcagaactccaaattctcttgttacaaagtaatgtttatgagaacatcccagaagttttttttcaaagtctacagtcattaagggtcttagatcttagccagactcgcatttctttcctacccggatcattcaatcttctaagcagcctccacactctgtgtctaaatggttgcctgttaatgacaagtgacatatctctactgggaagattgagggaacttaagattcttagtatgagagaacttcgtcttgtgaaattgccagaagaaataggacaattgaccaacctaaggatgctggacttcactagtgaaggtgttgatatagttccttctaatgtgataggcaacctcagaaaattagaagaactgtacatgcagtgcaattttgcggactgggagagtgaagttgaggagagcgaagctgagggagcaagagaggaaactaatgctttctttgatgagttaactggcttacatctacatattttgaaggttttcatatctgatccaaaatgcttgcctacaaatgtagtggttatgccgaattggaaaagttttgatatctgtatcagtagtgaccgctcaattagagaatcgcttttgcatccaagttctccacttgattcaaaagccttgactcttgacacaaccatggataccttaccgaattggttttttgaggtggtgaccaagaaaacagagaagctacagtacataaactgttggggtttacgcaatcttcttgaggaatatttccaagggacattacatgcactgaagtatttacatgttattggtcccattgagatggaacagttgatgaccccaacaacacctgatcgaaatgaacgtgtgtttgagaatttggaagagttgcatctcgatagagtgaaatgtctgaaagagttatgtgttggtgaattacaaactgagtctctgtgcaagcttaggttactgaaggtgagtcattgtcataaattgatgaatgcacttctacaatctaatttgttgcagagactacaaaatctggaaatactaatttgtgaagagatggatgaattggagtatgtgtttgcatgtgaagtgtttgacgcagaacaaattactctgagaaaattgatagaaatgagattggggaagctagggagcctgataaagatatggaatggtcctgctccttgtgaaatcttttgtcatgttaaaattttggtggttttcgagtgctataagctgaaaaatctcttcacatctgacatagctcaatgtcttcatgaattggaagatctttcagtagaggcttgccaagcgttggagagagtttttgaagcaagtaaggaaacagtgaacgacaagataattttaccaaaattgaagatcttagctttgaaacagcttccgaagcttacaaggttctatggtagtactggaagtgctactaatgatgaagaaaatattgagtttcctttattggaacactcgcacgtggaccgctgccataatttcaaatctccagctatggacatgttggtttcagtgatgaataagcagcctgttttgattattatgcaagagacaggtcagagagagacacctactttaagcagaagaagatga

Protein Analysis

1283

Amino Acids

145.75

Weight (kDa)

5.67

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 169 - 334 4.1e-48 TIR domain
TIR_2 PF13676 172 - 268 6.7e-18 TIR domain
NB-ARC PF00931 356 - 540 2.1e-24 NB-ARC domain
WHD_DRP PF23559 626 - 693 1.6e-08 Disease resistance protein Winged helix domain
LRR_8 PF13855 747 - 805 6.2e-08 Leucine rich repeat
LRR_14 PF23598 796 - 1041 4.1e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 1035 - 1135 5.8e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1121 - 1249 7.1e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 73
AccB1I GGYRCC 1 cut(s) 2106
AccB7I CCANNNNNTGG 3 cut(s) 437, 1242, 3547
AccBSI CCGCTC 3 cut(s) 892, 1973, 2820
AccI GTMKAC 2 cut(s) 1125, 2305
AccII CGCG 1 cut(s) 1380
AclI AACGTT 1 cut(s) 602
AclWI GGATC 6 cut(s) 461, 474, 488, 642, 2368, 2738
AcoI YGGCCR 2 cut(s) 1729, 2150
AcsI RAATTY 8 cut(s) 1049, 1346, 1364, 1467, 1600, 2247, 3100, 3441
AcuI CTGAAG 4 cut(s) 1806, 1937, 3035, 3215
AcvI CACGTG 1 cut(s) 3721
AcyI GRCGYC 4 cut(s) 70, 1140, 1763, 1965
AdeI CACNNNGTG 1 cut(s) 2394
AfaI GTAC 5 cut(s) 327, 813, 2609, 2951, 3661
AfiI CCNNNNNNNGG 8 cut(s) 437, 1189, 1242, 1260, 1738, 2020, 2526, 3547
AflII CTTAAG 1 cut(s) 2456
AflIII ACRYGT 6 cut(s) 1165, 1332, 1798, 3025, 3088, 3759
AgeI ACCGGT 1 cut(s) 781
AhlI ACTAGT 1 cut(s) 2543
AjiI CACGTC 1 cut(s) 1799
AjnI CCWGG 3 cut(s) 83, 462, 2152
AjuI GAANNNNNNNTTGG 2 cut(s) 1844, 1876
AleI CACNNNNGTG 1 cut(s) 721
Alw26I GTCTC 7 cut(s) 34, 47, 282, 3177, 3247, 3803, 3818
AlwI GGATC 6 cut(s) 461, 474, 488, 642, 2368, 2738
AoxI GGCC 4 cut(s) 86, 1004, 1729, 2150
ApeKI GCWGC 6 cut(s) 364, 1745, 1872, 2379, 3729, 3784
ApoI RAATTY 8 cut(s) 1049, 1346, 1364, 1467, 1600, 2247, 3100, 3441
ArsI GACNNNNNNTTYG 2 cut(s) 1349, 1381
AsiGI ACCGGT 1 cut(s) 781
Asp700I GAANNNNTTC 1 cut(s) 2732
AspLEI GCGC 2 cut(s) 1093, 2175
AspS9I GGNCC 6 cut(s) 157, 460, 1990, 3035, 3407, 3724
AsuC2I CCSGG 1 cut(s) 2358
AsuHPI GGTGA 8 cut(s) 47, 708, 1219, 1507, 1553, 2935, 3167, 3211
AsuII TTCGAA 2 cut(s) 500, 598
AvaII GGWCC 6 cut(s) 157, 460, 1990, 3035, 3407, 3724
AxyI CCTNAGG 1 cut(s) 2525
BaeGI GKGCMC 1 cut(s) 2109
BalI TGGCCA 1 cut(s) 2152
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 1 cut(s) 2106
BanII GRGCYC 1 cut(s) 1378
BarI GAAGNNNNNNTAC 2 cut(s) 2933, 2965
BbrPI CACGTG 1 cut(s) 3721
BbsI GAAGAC 3 cut(s) 1670, 1914, 3497
BbvCI CCTCAGC 1 cut(s) 2661
BbvI GCAGC 6 cut(s) 376, 1732, 1859, 2391, 3716, 3796
BccI CCATC 7 cut(s) 298, 385, 578, 2018, 2096, 3040, 3283
BceAI ACGGC 5 cut(s) 129, 830, 884, 1100, 1144
BcgI CGANNNNNNTGC 2 cut(s) 480, 514
BciT130I CCWGG 3 cut(s) 85, 464, 2154
BciVI GTATCC 2 cut(s) 568, 2887
BcnI CCSGG 1 cut(s) 2358
BcoDI GTCTC 7 cut(s) 34, 47, 282, 3177, 3247, 3803, 3818
BcuI ACTAGT 1 cut(s) 2543
BfaI CTAG 7 cut(s) 285, 629, 1521, 1749, 2010, 2544, 3380
BfmI CTRYAG 4 cut(s) 24, 1173, 2306, 2945
BfoI RGCGCY 2 cut(s) 1094, 2176
BfrI CTTAAG 1 cut(s) 2456
BfuI GTATCC 2 cut(s) 568, 2887
BglI GCCNNNNNGGC 1 cut(s) 1973
BglII AGATCT 3 cut(s) 2326, 3520, 3612
BisI GCNGC 8 cut(s) 142, 365, 1746, 1873, 1974, 2380, 3730, 3785
BlsI GCNGC 8 cut(s) 143, 366, 1747, 1874, 1975, 2381, 3731, 3786
BmcAI AGTACT 1 cut(s) 3661
Bme1390I CCNGG 4 cut(s) 85, 464, 2154, 2358
Bme18I GGWCC 6 cut(s) 157, 460, 1990, 3035, 3407, 3724
BmgBI CACGTC 1 cut(s) 1799
BmgT120I GGNCC 6 cut(s) 157, 460, 1990, 3035, 3407, 3724
BmiI GGNNCC 5 cut(s) 117, 468, 2108, 3037, 3386
BmrFI CCNGG 4 cut(s) 85, 464, 2154, 2358
BmrI ACTGGG 4 cut(s) 371, 1467, 2447, 2641
BmsI GCATC 9 cut(s) 194, 233, 826, 2033, 2043, 2152, 2520, 2851, 3124
BmuI ACTGGG 4 cut(s) 371, 1467, 2447, 2641
BoxI GACNNNNGTC 1 cut(s) 543
BpiI GAAGAC 3 cut(s) 1670, 1914, 3497
BpmI CTGGAG 5 cut(s) 102, 201, 908, 1018, 3732
Bpu10I CCTNAGC 4 cut(s) 426, 1579, 2661, 3185
Bpu14I TTCGAA 2 cut(s) 500, 598
BpuEI CTTGAG 1 cut(s) 3002
BpuMI CCSGG 1 cut(s) 2358
BsaAI YACGTR 1 cut(s) 3721
BsaHI GRCGYC 4 cut(s) 70, 1140, 1763, 1965
BsaJI CCNNGG 7 cut(s) 172, 719, 1071, 1155, 1503, 2889, 2995
BsaWI WCCGGW 2 cut(s) 32, 781
BsaXI ACNNNNNCTCC 4 cut(s) 309, 339, 3293, 3323
Bsc4I CCNNNNNNNGG 8 cut(s) 437, 1189, 1242, 1260, 1738, 2020, 2526, 3547
Bse118I RCCGGY 1 cut(s) 781
Bse21I CCTNAGG 1 cut(s) 2525
Bse3DI GCAATG 2 cut(s) 201, 1644
BseBI CCWGG 3 cut(s) 85, 464, 2154
BseDI CCNNGG 7 cut(s) 172, 719, 1071, 1155, 1503, 2889, 2995
BseLI CCNNNNNNNGG 8 cut(s) 437, 1189, 1242, 1260, 1738, 2020, 2526, 3547
BseMI GCAATG 2 cut(s) 201, 1644
BseMII CTCAG 6 cut(s) 417, 512, 2602, 2652, 3159, 3338
BseRI GAGGAG 3 cut(s) 35, 161, 2663
BseSI GKGCMC 1 cut(s) 2109
BseXI GCAGC 6 cut(s) 376, 1732, 1859, 2391, 3716, 3796
BsgI GTGCAG 1 cut(s) 555
Bsh1236I CGCG 1 cut(s) 1380
BshFI GGCC 4 cut(s) 88, 1006, 1731, 2152
BshNI GGYRCC 1 cut(s) 2106
BshTI ACCGGT 1 cut(s) 781
BsiSI CCGG 3 cut(s) 33, 782, 2358
BslFI GGGAC 5 cut(s) 92, 1981, 2048, 3013, 3021
BslI CCNNNNNNNGG 8 cut(s) 437, 1189, 1242, 1260, 1738, 2020, 2526, 3547
BsmAI GTCTC 7 cut(s) 34, 47, 282, 3177, 3247, 3803, 3818
BsmBI CGTCTC 1 cut(s) 282
BsmFI GGGAC 5 cut(s) 92, 1981, 2048, 3013, 3021
BsmI GAATGC 3 cut(s) 97, 1447, 3229
BsnI GGCC 4 cut(s) 88, 1006, 1731, 2152
Bsp119I TTCGAA 2 cut(s) 500, 598
Bsp1286I GDGCHC 2 cut(s) 1378, 2109
Bsp1407I TGTACA 2 cut(s) 325, 2607
Bsp143I GATC 9 cut(s) 466, 493, 634, 2326, 2360, 2743, 3076, 3520, 3612
Bsp19I CCATGG 4 cut(s) 719, 1071, 1155, 2889
BspANI GGCC 4 cut(s) 88, 1006, 1731, 2152
BspCNI CTCAG 6 cut(s) 418, 513, 2601, 2653, 3160, 3339
BspFNI CGCG 1 cut(s) 1380
BspHI TCATGA 1 cut(s) 3508
BspLI GGNNCC 5 cut(s) 117, 468, 2108, 3037, 3386
BspPI GGATC 6 cut(s) 461, 474, 488, 642, 2368, 2738
BspT104I TTCGAA 2 cut(s) 500, 598
BspT107I GGYRCC 1 cut(s) 2106
BspTI CTTAAG 1 cut(s) 2456
BsrBI CCGCTC 3 cut(s) 892, 1973, 2820
BsrDI GCAATG 2 cut(s) 201, 1644
BsrFI RCCGGY 1 cut(s) 781
BsrGI TGTACA 2 cut(s) 325, 2607
BssAI RCCGGY 1 cut(s) 781
BssECI CCNNGG 7 cut(s) 172, 719, 1071, 1155, 1503, 2889, 2995
BssMI GATC 9 cut(s) 466, 493, 634, 2326, 2360, 2743, 3076, 3520, 3612
BssNI GRCGYC 4 cut(s) 70, 1140, 1763, 1965
BssT1I CCWWGG 6 cut(s) 719, 1071, 1155, 1503, 2889, 2995
Bst2UI CCWGG 3 cut(s) 85, 464, 2154
Bst4CI ACNGT 9 cut(s) 106, 916, 2115, 2310, 2607, 2949, 2960, 3054, 3580
Bst6I CTCTTC 6 cut(s) 249, 288, 486, 3102, 3279, 3485
BstACI GRCGYC 4 cut(s) 70, 1140, 1763, 1965
BstAFI CTTAAG 1 cut(s) 2456
BstAPI GCANNNNNTGC 1 cut(s) 191
BstAUI TGTACA 2 cut(s) 325, 2607
BstBAI YACGTR 1 cut(s) 3721
BstBI TTCGAA 2 cut(s) 500, 598
BstC8I GCNNGC 5 cut(s) 1378, 2213, 2756, 3182, 3538
BstDSI CCRYGG 4 cut(s) 719, 1071, 1155, 2889
BstEII GGTNACC 1 cut(s) 2923
BstFNI CGCG 1 cut(s) 1380
BstH2I RGCGCY 2 cut(s) 1094, 2176
BstHHI GCGC 2 cut(s) 1093, 2175
BstKTI GATC 9 cut(s) 469, 496, 637, 2329, 2363, 2746, 3079, 3523, 3615
BstMAI GTCTC 7 cut(s) 34, 47, 282, 3177, 3247, 3803, 3818
BstMBI GATC 9 cut(s) 466, 493, 634, 2326, 2360, 2743, 3076, 3520, 3612
BstNI CCWGG 3 cut(s) 85, 464, 2154
BstNSI RCATGY 8 cut(s) 1169, 1336, 2215, 2614, 3011, 3029, 3318, 3763
BstPAI GACNNNNGTC 1 cut(s) 543
BstPI GGTNACC 1 cut(s) 2923
BstSCI CCNGG 4 cut(s) 83, 462, 2152, 2356
BstSFI CTRYAG 4 cut(s) 24, 1173, 2306, 2945
BstSLI GKGCMC 1 cut(s) 2109
BstUI CGCG 1 cut(s) 1380
BstV1I GCAGC 6 cut(s) 376, 1732, 1859, 2391, 3716, 3796
BstV2I GAAGAC 3 cut(s) 1670, 1914, 3497
BstX2I RGATCY 5 cut(s) 466, 493, 2326, 3520, 3612
BstXI CCANNNNNNTGG 2 cut(s) 1306, 3046
BstYI RGATCY 5 cut(s) 466, 493, 2326, 3520, 3612
Bsu36I CCTNAGG 1 cut(s) 2525
BsuI GTATCC 2 cut(s) 568, 2887
BsuRI GGCC 4 cut(s) 88, 1006, 1731, 2152
BtgI CCRYGG 4 cut(s) 719, 1071, 1155, 2889
BtrI CACGTC 1 cut(s) 1799
BtsI GCAGTG 3 cut(s) 147, 1110, 2621
BtsIMutI CAGTG 8 cut(s) 147, 384, 462, 1110, 2621, 3011, 3585, 3777
Cac8I GCNNGC 5 cut(s) 1378, 2213, 2756, 3182, 3538
CciI TCATGA 1 cut(s) 3508
CfoI GCGC 2 cut(s) 1093, 2175
Cfr10I RCCGGY 1 cut(s) 781
Cfr13I GGNCC 6 cut(s) 157, 460, 1990, 3035, 3407, 3724
CseI GACGC 5 cut(s) 1129, 1503, 1752, 1973, 3338
Csp6I GTAC 5 cut(s) 326, 812, 2608, 2950, 3660
CspAI ACCGGT 1 cut(s) 781
CspCI CAANNNNNGTGG 4 cut(s) 1140, 1175, 2751, 2786
CviQI GTAC 5 cut(s) 326, 812, 2608, 2950, 3660
DpnI GATC 9 cut(s) 468, 495, 636, 2328, 2362, 2745, 3078, 3522, 3614
DpnII GATC 9 cut(s) 466, 493, 634, 2326, 2360, 2743, 3076, 3520, 3612
DraI TTTAAA 2 cut(s) 909, 1807
DraIII CACNNNGTG 1 cut(s) 2394
EaeI YGGCCR 2 cut(s) 1729, 2150
Eam1104I CTCTTC 6 cut(s) 249, 288, 486, 3102, 3279, 3485
EarI CTCTTC 6 cut(s) 249, 288, 486, 3102, 3279, 3485
Eco130I CCWWGG 6 cut(s) 719, 1071, 1155, 1503, 2889, 2995
Eco147I AGGCCT 1 cut(s) 88
Eco24I GRGCYC 1 cut(s) 1378
Eco32I GATATC 2 cut(s) 1839, 2800
Eco47I GGWCC 6 cut(s) 157, 460, 1990, 3035, 3407, 3724
Eco57I CTGAAG 4 cut(s) 1806, 1937, 3035, 3215
Eco72I CACGTG 1 cut(s) 3721
Eco81I CCTNAGG 1 cut(s) 2525
Eco91I GGTNACC 1 cut(s) 2923
EcoO65I GGTNACC 1 cut(s) 2923
EcoRI GAATTC 1 cut(s) 1467
EcoRII CCWGG 3 cut(s) 83, 462, 2152
EcoRV GATATC 2 cut(s) 1839, 2800
EcoT14I CCWWGG 6 cut(s) 719, 1071, 1155, 1503, 2889, 2995
EcoT22I ATGCAT 4 cut(s) 758, 1814, 2048, 2058
EcoT38I GRGCYC 1 cut(s) 1378
ErhI CCWWGG 6 cut(s) 719, 1071, 1155, 1503, 2889, 2995
Esp3I CGTCTC 1 cut(s) 282
FalI AAGNNNNNCTT 8 cut(s) 1223, 1255, 1241, 1273, 1259, 1291, 1286, 1318
FaqI GGGAC 5 cut(s) 92, 1981, 2048, 3013, 3021
FauI CCCGC 1 cut(s) 1385
FblI GTMKAC 2 cut(s) 1125, 2305
Fnu4HI GCNGC 8 cut(s) 142, 365, 1746, 1873, 1974, 2380, 3730, 3785
FriOI GRGCYC 1 cut(s) 1378
Fsp4HI GCNGC 8 cut(s) 142, 365, 1746, 1873, 1974, 2380, 3730, 3785
FspBI CTAG 7 cut(s) 285, 629, 1521, 1749, 2010, 2544, 3380
GlaI GCGC 2 cut(s) 1092, 2174
GluI GCNGC 8 cut(s) 142, 365, 1746, 1873, 1974, 2380, 3730, 3785
GsuI CTGGAG 5 cut(s) 102, 201, 908, 1018, 3732
HaeII RGCGCY 2 cut(s) 1094, 2176
HaeIII GGCC 4 cut(s) 88, 1006, 1731, 2152
HapII CCGG 3 cut(s) 33, 782, 2358
HgaI GACGC 5 cut(s) 1129, 1503, 1752, 1973, 3338
HhaI GCGC 2 cut(s) 1093, 2175
Hin1I GRCGYC 4 cut(s) 70, 1140, 1763, 1965
Hin6I GCGC 2 cut(s) 1091, 2173
HinP1I GCGC 2 cut(s) 1091, 2173
HincII GTYRAC 3 cut(s) 1357, 1455, 2703
HindII GTYRAC 3 cut(s) 1357, 1455, 2703
HindIII AAGCTT 4 cut(s) 620, 903, 3182, 3638
HpaI GTTAAC 1 cut(s) 2703
HpaII CCGG 3 cut(s) 33, 782, 2358
HphI GGTGA 8 cut(s) 47, 708, 1219, 1507, 1553, 2935, 3167, 3211
Hpy99I CGWCG 4 cut(s) 115, 130, 1145, 2115
HpyCH4III ACNGT 9 cut(s) 106, 916, 2115, 2310, 2607, 2949, 2960, 3054, 3580
HpyCH4IV ACGT 5 cut(s) 70, 602, 1798, 3088, 3720
HpySE526I ACGT 5 cut(s) 70, 602, 1798, 3088, 3720
Hsp92I GRCGYC 4 cut(s) 70, 1140, 1763, 1965
HspAI GCGC 2 cut(s) 1091, 2173
KspAI GTTAAC 1 cut(s) 2703
Kzo9I GATC 9 cut(s) 466, 493, 634, 2326, 2360, 2743, 3076, 3520, 3612
LmnI GCTCC 7 cut(s) 25, 121, 182, 889, 2666, 3384, 3418
Lsp1109I GCAGC 6 cut(s) 376, 1732, 1859, 2391, 3716, 3796
LweI GCATC 9 cut(s) 194, 233, 826, 2033, 2043, 2152, 2520, 2851, 3124
MaeI CTAG 7 cut(s) 285, 629, 1521, 1749, 2010, 2544, 3380
MaeII ACGT 5 cut(s) 70, 602, 1798, 3088, 3720
MaeIII GTNAC 8 cut(s) 1108, 1475, 1553, 2256, 2423, 2813, 2923, 3189
MalI GATC 9 cut(s) 468, 495, 636, 2328, 2362, 2745, 3078, 3522, 3614
MbiI CCGCTC 3 cut(s) 892, 1973, 2820
MboI GATC 9 cut(s) 466, 493, 634, 2326, 2360, 2743, 3076, 3520, 3612
MfeI CAATTG 3 cut(s) 663, 684, 2513
MflI RGATCY 5 cut(s) 466, 493, 2326, 3520, 3612
MhlI GDGCHC 2 cut(s) 1378, 2109
MlsI TGGCCA 1 cut(s) 2152
MluNI TGGCCA 1 cut(s) 2152
MlyI GAGTC 5 cut(s) 1841, 2332, 2869, 3179, 3211
MmeI TCCRAC 4 cut(s) 295, 1123, 1287, 3528
Mox20I TGGCCA 1 cut(s) 2152
Mph1103I ATGCAT 4 cut(s) 758, 1814, 2048, 2058
MroXI GAANNNNTTC 1 cut(s) 2732
MscI TGGCCA 1 cut(s) 2152
MslI CAYNNNNRTG 4 cut(s) 721, 2051, 2888, 3044
Msp20I TGGCCA 1 cut(s) 2152
MspA1I CMGCKG 3 cut(s) 141, 388, 3729
MspCI CTTAAG 1 cut(s) 2456
MspI CCGG 3 cut(s) 33, 782, 2358
MspR9I CCNGG 4 cut(s) 85, 464, 2154, 2358
MunI CAATTG 3 cut(s) 663, 684, 2513
Mva1269I GAATGC 3 cut(s) 97, 1447, 3229
MvaI CCWGG 3 cut(s) 85, 464, 2154
MvnI CGCG 1 cut(s) 1380
NciI CCSGG 1 cut(s) 2358
NcoI CCATGG 4 cut(s) 719, 1071, 1155, 2889
NdeII GATC 9 cut(s) 466, 493, 634, 2326, 2360, 2743, 3076, 3520, 3612
NlaIV GGNNCC 5 cut(s) 117, 468, 2108, 3037, 3386
NmuCI GTSAC 5 cut(s) 1108, 1553, 2423, 2813, 2923
NsiI ATGCAT 4 cut(s) 758, 1814, 2048, 2058
NspI RCATGY 8 cut(s) 1169, 1336, 2215, 2614, 3011, 3029, 3318, 3763
NspV TTCGAA 2 cut(s) 500, 598
OliI CACNNNNGTG 1 cut(s) 721
PaeI GCATGC 1 cut(s) 2215
PagI TCATGA 1 cut(s) 3508
PceI AGGCCT 1 cut(s) 88
PciI ACATGT 4 cut(s) 1165, 1332, 3025, 3759
PcsI WCGNNNNNNNCGW 2 cut(s) 107, 3085
PctI GAATGC 3 cut(s) 97, 1447, 3229
PdmI GAANNNNTTC 1 cut(s) 2732
PflMI CCANNNNNTGG 3 cut(s) 437, 1242, 3547
PinAI ACCGGT 1 cut(s) 781
PkrI GCNGC 8 cut(s) 143, 366, 1747, 1874, 1975, 2381, 3731, 3786
PleI GAGTC 5 cut(s) 1841, 2332, 2869, 3178, 3210
PmaCI CACGTG 1 cut(s) 3721
PmlI CACGTG 1 cut(s) 3721
PpsI GAGTC 5 cut(s) 1841, 2332, 2869, 3178, 3210
Ppu21I YACGTR 1 cut(s) 3721
PscI ACATGT 4 cut(s) 1165, 1332, 3025, 3759
PshAI GACNNNNGTC 1 cut(s) 543
Psp1406I AACGTT 1 cut(s) 602
Psp6I CCWGG 3 cut(s) 83, 462, 2152
PspCI CACGTG 1 cut(s) 3721
PspEI GGTNACC 1 cut(s) 2923
PspGI CCWGG 3 cut(s) 83, 462, 2152
PspN4I GGNNCC 5 cut(s) 117, 468, 2108, 3037, 3386
PspPI GGNCC 6 cut(s) 157, 460, 1990, 3035, 3407, 3724
PsuI RGATCY 5 cut(s) 466, 493, 2326, 3520, 3612
PvuII CAGCTG 1 cut(s) 388
RsaI GTAC 5 cut(s) 327, 813, 2609, 2951, 3661
RsaNI GTAC 5 cut(s) 326, 812, 2608, 2950, 3660
RseI CAYNNNNRTG 4 cut(s) 721, 2051, 2888, 3044
SatI GCNGC 8 cut(s) 142, 365, 1746, 1873, 1974, 2380, 3730, 3785
Sau3AI GATC 9 cut(s) 466, 493, 634, 2326, 2360, 2743, 3076, 3520, 3612
Sau96I GGNCC 6 cut(s) 157, 460, 1990, 3035, 3407, 3724
ScaI AGTACT 1 cut(s) 3661
SchI GAGTC 5 cut(s) 1841, 2332, 2869, 3179, 3211
ScrFI CCNGG 4 cut(s) 85, 464, 2154, 2358
SduI GDGCHC 2 cut(s) 1378, 2109
SfaNI GCATC 9 cut(s) 194, 233, 826, 2033, 2043, 2152, 2520, 2851, 3124
SfcI CTRYAG 4 cut(s) 24, 1173, 2306, 2945
SfuI TTCGAA 2 cut(s) 500, 598
SinI GGWCC 6 cut(s) 157, 460, 1990, 3035, 3407, 3724
SmiI ATTTAAAT 1 cut(s) 1807
SmiMI CAYNNNNRTG 4 cut(s) 721, 2051, 2888, 3044
SmlI CTYRAG 2 cut(s) 2456, 2981
SmoI CTYRAG 2 cut(s) 2456, 2981
SpeI ACTAGT 1 cut(s) 2543
SphI GCATGC 1 cut(s) 2215
SseBI AGGCCT 1 cut(s) 88
SspI AATATT 2 cut(s) 2990, 3691
SspMI CTAG 7 cut(s) 285, 629, 1521, 1749, 2010, 2544, 3380
StuI AGGCCT 1 cut(s) 88
StyD4I CCNGG 4 cut(s) 83, 462, 2152, 2356
StyI CCWWGG 6 cut(s) 719, 1071, 1155, 1503, 2889, 2995
SwaI ATTTAAAT 1 cut(s) 1807
TaaI ACNGT 9 cut(s) 106, 916, 2115, 2310, 2607, 2949, 2960, 3054, 3580
TaiI ACGT 5 cut(s) 73, 605, 1801, 3091, 3723
TaqI TCGA 8 cut(s) 101, 125, 500, 598, 798, 3079, 3120, 3456
TatI WGTACW 4 cut(s) 325, 2607, 2949, 3659
TauI GCSGC 2 cut(s) 144, 1976
TscAI CASTG 8 cut(s) 154, 384, 462, 1117, 2621, 3018, 3585, 3777
TseFI GTSAC 5 cut(s) 1108, 1553, 2423, 2813, 2923
TseI GCWGC 6 cut(s) 364, 1745, 1872, 2379, 3729, 3784
Tsp45I GTSAC 5 cut(s) 1108, 1553, 2423, 2813, 2923
TspGWI ACGGA 3 cut(s) 143, 1190, 1374
TspRI CASTG 8 cut(s) 154, 384, 462, 1117, 2621, 3018, 3585, 3777
Van91I CCANNNNNTGG 3 cut(s) 437, 1242, 3547
Vha464I CTTAAG 1 cut(s) 2456
VpaK11BI GGWCC 6 cut(s) 157, 460, 1990, 3035, 3407, 3724
XapI RAATTY 8 cut(s) 1049, 1346, 1364, 1467, 1600, 2247, 3100, 3441
XceI RCATGY 8 cut(s) 1169, 1336, 2215, 2614, 3011, 3029, 3318, 3763
XmiI GTMKAC 2 cut(s) 1125, 2305
XmnI GAANNNNTTC 1 cut(s) 2732
XspI CTAG 7 cut(s) 285, 629, 1521, 1749, 2010, 2544, 3380
ZraI GACGTC 1 cut(s) 71
ZrmI AGTACT 1 cut(s) 3661
Zsp2I ATGCAT 4 cut(s) 758, 1814, 2048, 2058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.