Rmu_sc0001814.1_g000020

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001814.1
OG0014643
Physical Location & Seq
Forward (+)
111155 .. 112651
1497 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1497 bp
atggaagttgagctcattgatcacccattcaacccgagctacgacctctatgactgtagctttgaatatgattcgtccttccaaacatcagatttatttactccttgtaccaccaatgttgagcaaaactcggaccacgagtgcctcaacagattgctccaaatagaagcacagctcatggacatagactcgtctatcagcagtactggaagtgatgtaattggtcttgatggtggtgttgatagtgaattcgcttcttacataagtgaagaagcaccagaaaatgtccatgtttcgacagaatccaactcccttttgaaggagattcagatagatttgctggaagagagcagcttaactgaacttttattcgcaggagctgaagcagttgaagcacagaactgggtttttgcttcaaatataatcacaaagctcaacactcttctagccggtagcgaaaatggagacaactcgttcaacaagttggccacgttcttcactcaaggccttcattacaagaccattaactctcctgaagttccccatgagccagctgtttctagaaacacagacacaatgtcttcatttcagatgctccaagagctctctccttttgttaaatttgcgcatttcacagccaaccaagcgattctcgaggccacacaagaggatgaggacattcatgtcattgactttgacatcatggagggtagccagtggccttcattgatggttgaccttgcaatgaggaaaggtcgctgctgtgcttcactcaaggtaaccgcagtcacagttgaccagcaaagcgccgctcttgctcaacaaactggaagaaggcttaaagagttctcggagtctatcaatttgttatttatttttgatcatatgatgatggccacagaagaagattttgctaaaatcgaagttgttgctgatgcagtagtagccaattgcatgattcaccagcttcacatgcctaacagaagtatctcattagtgaaggccttcttgggtggcatgagcaaattatcacctaaactcattgtcctagtacaagaagagttgttcaacttttccaagatgccatcaatgttctttgcagagttcttctccgaggctctccatcactacaattcgctttgcgactctcttttaactagttttagtggtgagtataaaatggggttaagacagatagagaaagaggttttgaggatcaagattttagaaagtgtcaggcagttcgcatgtgagaaggaggatagaatgttgtgggaagattgttttgcttcgttgaagggcttcaagtctatccctttaagttcttgtaatgtttctcaagctaaatacttggtgagtcttttcaataggggatattgggtgcagcatgagaagggcaggttagctttgtgttggaaatcaagacctttgacaacagcttccatttgggtgcccttaacttacaaaatcaaggaaaaccatgtcaaccccttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

498

Amino Acids

56.19

Weight (kDa)

4.78

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 120 - 483 2.8e-76 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 683
Acc16I TGCGCA 1 cut(s) 627
Acc36I ACCTGC 1 cut(s) 1389
AccB1I GGYRCC 1 cut(s) 1450
AccBSI CCGCTC 1 cut(s) 812
AciI CCGC 2 cut(s) 783, 810
AclWI GGATC 1 cut(s) 1223
AcoI YGGCCR 2 cut(s) 486, 894
AcsI RAATTY 2 cut(s) 248, 620
AcuI CTGAAG 2 cut(s) 402, 555
AfaI GTAC 3 cut(s) 109, 205, 1053
AfiI CCNNNNNNNGG 1 cut(s) 319
AhdI GACNNNNNGTC 1 cut(s) 577
AhlI ACTAGT 1 cut(s) 1157
AjuI GAANNNNNNNTTGG 2 cut(s) 992, 1024
Alw21I GWGCWC 2 cut(s) 15, 606
Alw26I GTCTC 1 cut(s) 459
AlwI GGATC 1 cut(s) 1223
AlwNI CAGNNNCTG 1 cut(s) 380
Ama87I CYCGRG 2 cut(s) 34, 653
AoxI GGCC 6 cut(s) 486, 505, 657, 719, 894, 1002
ApeKI GCWGC 3 cut(s) 351, 759, 1384
ApoI RAATTY 2 cut(s) 248, 620
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
Asp700I GAANNNNTTC 2 cut(s) 1004, 1301
AspLEI GCGC 2 cut(s) 628, 809
AspS9I GGNCC 1 cut(s) 133
AsuHPI GGTGA 5 cut(s) 14, 953, 1023, 1181, 1366
AvaI CYCGRG 2 cut(s) 34, 653
AvaII GGWCC 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 1455
BaeI ACNNNNGTAYC 2 cut(s) 970, 1003
BalI TGGCCA 2 cut(s) 488, 896
BanI GGYRCC 1 cut(s) 1450
BanII GRGCYC 2 cut(s) 15, 606
BauI CACGAG 1 cut(s) 137
BbsI GAAGAC 1 cut(s) 573
Bbv12I GWGCWC 2 cut(s) 15, 606
BbvI GCAGC 3 cut(s) 363, 746, 1396
BccI CCATC 5 cut(s) 224, 724, 886, 1093, 1131
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BclI TGATCA 2 cut(s) 19, 880
BcoDI GTCTC 1 cut(s) 459
BcuI ACTAGT 1 cut(s) 1157
BfaI CTAG 4 cut(s) 446, 561, 1049, 1158
BfmI CTRYAG 1 cut(s) 55
BfoI RGCGCY 1 cut(s) 810
BfuAI ACCTGC 1 cut(s) 1389
BisI GCNGC 4 cut(s) 352, 760, 810, 1385
BlsI GCNGC 4 cut(s) 353, 761, 811, 1386
BmcAI AGTACT 1 cut(s) 205
Bme18I GGWCC 1 cut(s) 133
BmeRI GACNNNNNGTC 1 cut(s) 577
BmeT110I CYCGRG 2 cut(s) 34, 653
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 1452
BmrI ACTGGG 1 cut(s) 412
BmsI GCATC 3 cut(s) 582, 925, 1071
BmuI ACTGGG 1 cut(s) 412
BpiI GAAGAC 1 cut(s) 573
BplI GAGNNNNNCTC 4 cut(s) 113, 145, 1094, 1126
BpuEI CTTGAG 3 cut(s) 486, 758, 1323
BsaJI CCNNGG 1 cut(s) 1113
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse118I RCCGGY 1 cut(s) 449
Bse1I ACTGG 4 cut(s) 211, 407, 715, 832
Bse3DI GCAATG 1 cut(s) 750
BseDI CCNNGG 1 cut(s) 1113
BseGI GGATG 1 cut(s) 676
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMI GCAATG 1 cut(s) 750
BseNI ACTGG 4 cut(s) 211, 407, 715, 832
BseSI GKGCMC 1 cut(s) 1455
BseXI GCAGC 3 cut(s) 363, 746, 1396
BsgI GTGCAG 1 cut(s) 1403
BshFI GGCC 6 cut(s) 488, 507, 659, 721, 896, 1004
BshNI GGYRCC 1 cut(s) 1450
BsiHKAI GWGCWC 2 cut(s) 15, 606
BsiHKCI CYCGRG 2 cut(s) 34, 653
BsiSI CCGG 1 cut(s) 450
BslI CCNNNNNNNGG 1 cut(s) 319
BsmAI GTCTC 1 cut(s) 459
BsnI GGCC 6 cut(s) 488, 507, 659, 721, 896, 1004
BsoBI CYCGRG 2 cut(s) 34, 653
Bsp1286I GDGCHC 3 cut(s) 15, 606, 1455
Bsp143I GATC 3 cut(s) 19, 880, 1215
BspACI CCGC 2 cut(s) 783, 810
BspANI GGCC 6 cut(s) 488, 507, 659, 721, 896, 1004
BspLI GGNNCC 1 cut(s) 1452
BspMI ACCTGC 1 cut(s) 1389
BspPI GGATC 1 cut(s) 1223
BspT107I GGYRCC 1 cut(s) 1450
BsrBI CCGCTC 1 cut(s) 812
BsrDI GCAATG 1 cut(s) 750
BsrFI RCCGGY 1 cut(s) 449
BsrI ACTGG 4 cut(s) 211, 407, 715, 832
BssAI RCCGGY 1 cut(s) 449
BssECI CCNNGG 1 cut(s) 1113
BssMI GATC 3 cut(s) 19, 880, 1215
BssSI CACGAG 1 cut(s) 137
Bst2BI CACGAG 1 cut(s) 137
Bst4CI ACNGT 2 cut(s) 56, 793
Bst6I CTCTTC 3 cut(s) 339, 447, 1053
BstC8I GCNNGC 1 cut(s) 552
BstEII GGTNACC 1 cut(s) 778
BstENI CCTNNNNNAGG 1 cut(s) 317
BstF5I GGATG 1 cut(s) 676
BstH2I RGCGCY 1 cut(s) 810
BstHHI GCGC 2 cut(s) 628, 809
BstKTI GATC 3 cut(s) 22, 883, 1218
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 3 cut(s) 19, 880, 1215
BstMWI GCNNNNNNNGC 6 cut(s) 392, 601, 644, 815, 944, 973
BstNSI RCATGY 2 cut(s) 976, 1251
BstPI GGTNACC 1 cut(s) 778
BstSFI CTRYAG 1 cut(s) 55
BstSLI GKGCMC 1 cut(s) 1455
BstV1I GCAGC 3 cut(s) 363, 746, 1396
BstV2I GAAGAC 1 cut(s) 573
BsuRI GGCC 6 cut(s) 488, 507, 659, 721, 896, 1004
BtsCI GGATG 1 cut(s) 676
BtsIMutI CAGTG 1 cut(s) 722
BveI ACCTGC 1 cut(s) 1389
Cac8I GCNNGC 1 cut(s) 552
CaiI CAGNNNCTG 1 cut(s) 380
CfoI GCGC 2 cut(s) 628, 809
Cfr10I RCCGGY 1 cut(s) 449
Cfr13I GGNCC 1 cut(s) 133
Csp6I GTAC 3 cut(s) 108, 204, 1052
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviQI GTAC 3 cut(s) 108, 204, 1052
DpnI GATC 3 cut(s) 21, 882, 1217
DpnII GATC 3 cut(s) 19, 880, 1215
DrdI GACNNNNNNGTC 1 cut(s) 683
DriI GACNNNNNGTC 1 cut(s) 577
DseDI GACNNNNNNGTC 1 cut(s) 683
EaeI YGGCCR 2 cut(s) 486, 894
Eam1104I CTCTTC 3 cut(s) 339, 447, 1053
Eam1105I GACNNNNNGTC 1 cut(s) 577
EarI CTCTTC 3 cut(s) 339, 447, 1053
Ecl136II GAGCTC 2 cut(s) 13, 604
Eco147I AGGCCT 2 cut(s) 507, 1004
Eco24I GRGCYC 2 cut(s) 15, 606
Eco47I GGWCC 1 cut(s) 133
Eco53kI GAGCTC 2 cut(s) 13, 604
Eco57I CTGAAG 2 cut(s) 402, 555
Eco88I CYCGRG 2 cut(s) 34, 653
Eco91I GGTNACC 1 cut(s) 778
EcoICRI GAGCTC 2 cut(s) 13, 604
EcoNI CCTNNNNNAGG 1 cut(s) 317
EcoO65I GGTNACC 1 cut(s) 778
EcoRI GAATTC 1 cut(s) 248
EcoT38I GRGCYC 2 cut(s) 15, 606
FalI AAGNNNNNCTT 2 cut(s) 992, 1024
FauNDI CATATG 1 cut(s) 885
FbaI TGATCA 2 cut(s) 19, 880
Fnu4HI GCNGC 4 cut(s) 352, 760, 810, 1385
FokI GGATG 1 cut(s) 683
FriOI GRGCYC 2 cut(s) 15, 606
Fsp4HI GCNGC 4 cut(s) 352, 760, 810, 1385
FspBI CTAG 4 cut(s) 446, 561, 1049, 1158
FspI TGCGCA 1 cut(s) 627
GlaI GCGC 2 cut(s) 627, 808
GluI GCNGC 4 cut(s) 352, 760, 810, 1385
HaeII RGCGCY 1 cut(s) 810
HaeIII GGCC 6 cut(s) 488, 507, 659, 721, 896, 1004
HapII CCGG 1 cut(s) 450
HhaI GCGC 2 cut(s) 628, 809
Hin6I GCGC 2 cut(s) 626, 807
HinP1I GCGC 2 cut(s) 626, 807
HincII GTYRAC 3 cut(s) 736, 796, 1486
HindII GTYRAC 3 cut(s) 736, 796, 1486
HinfI GANTC 9 cut(s) 71, 188, 302, 325, 649, 854, 958, 1145, 1357
HpaII CCGG 1 cut(s) 450
HphI GGTGA 5 cut(s) 14, 953, 1023, 1181, 1366
Hpy166II GTNNAC 3 cut(s) 736, 796, 1486
Hpy188I TCNGA 6 cut(s) 91, 133, 330, 591, 853, 1114
Hpy188III TCNNGA 6 cut(s) 227, 533, 561, 653, 1219, 1422
Hpy8I GTNNAC 3 cut(s) 736, 796, 1486
HpyCH4III ACNGT 2 cut(s) 56, 793
HpyCH4IV ACGT 1 cut(s) 491
HpyCH4V TGCA 5 cut(s) 743, 938, 954, 1100, 1384
HpyF10VI GCNNNNNNNGC 6 cut(s) 392, 601, 644, 815, 944, 973
HpySE526I ACGT 1 cut(s) 491
HspAI GCGC 2 cut(s) 626, 807
Ksp22I TGATCA 2 cut(s) 19, 880
Kzo9I GATC 3 cut(s) 19, 880, 1215
LmnI GCTCC 3 cut(s) 162, 377, 600
Lsp1109I GCAGC 3 cut(s) 363, 746, 1396
LweI GCATC 3 cut(s) 582, 925, 1071
MaeI CTAG 4 cut(s) 446, 561, 1049, 1158
MaeII ACGT 1 cut(s) 491
MaeIII GTNAC 2 cut(s) 778, 787
MalI GATC 3 cut(s) 21, 882, 1217
MbiI CCGCTC 1 cut(s) 812
MboI GATC 3 cut(s) 19, 880, 1215
MfeI CAATTG 1 cut(s) 949
MhlI GDGCHC 3 cut(s) 15, 606, 1455
MlsI TGGCCA 2 cut(s) 488, 896
MluCI AATT 7 cut(s) 219, 248, 620, 862, 949, 1025, 1132
MluNI TGGCCA 2 cut(s) 488, 896
MlyI GAGTC 4 cut(s) 182, 863, 1139, 1366
MmeI TCCRAC 2 cut(s) 330, 1394
Mox20I TGGCCA 2 cut(s) 488, 896
MroXI GAANNNNTTC 2 cut(s) 1004, 1301
MscI TGGCCA 2 cut(s) 488, 896
MseI TTAA 8 cut(s) 356, 525, 618, 840, 1154, 1187, 1319, 1457
MslI CAYNNNNRTG 1 cut(s) 1448
Msp20I TGGCCA 2 cut(s) 488, 896
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 1 cut(s) 450
MunI CAATTG 1 cut(s) 949
MwoI GCNNNNNNNGC 6 cut(s) 392, 601, 644, 815, 944, 973
NdeI CATATG 1 cut(s) 885
NdeII GATC 3 cut(s) 19, 880, 1215
NlaIV GGNNCC 1 cut(s) 1452
NmuCI GTSAC 1 cut(s) 787
NsbI TGCGCA 1 cut(s) 627
NspI RCATGY 2 cut(s) 976, 1251
PaeR7I CTCGAG 1 cut(s) 653
PceI AGGCCT 2 cut(s) 507, 1004
PdmI GAANNNNTTC 2 cut(s) 1004, 1301
PfeI GAWTC 5 cut(s) 71, 302, 325, 649, 958
PkrI GCNGC 4 cut(s) 353, 761, 811, 1386
PleI GAGTC 4 cut(s) 182, 862, 1139, 1365
PpsI GAGTC 4 cut(s) 182, 862, 1139, 1365
Psp124BI GAGCTC 2 cut(s) 15, 606
PspEI GGTNACC 1 cut(s) 778
PspN4I GGNNCC 1 cut(s) 1452
PspPI GGNCC 1 cut(s) 133
PstNI CAGNNNCTG 1 cut(s) 380
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 3 cut(s) 109, 205, 1053
RsaNI GTAC 3 cut(s) 108, 204, 1052
RseI CAYNNNNRTG 1 cut(s) 1448
SacI GAGCTC 2 cut(s) 15, 606
SaqAI TTAA 8 cut(s) 356, 525, 618, 840, 1154, 1187, 1319, 1457
SatI GCNGC 4 cut(s) 352, 760, 810, 1385
Sau3AI GATC 3 cut(s) 19, 880, 1215
Sau96I GGNCC 1 cut(s) 133
ScaI AGTACT 1 cut(s) 205
SchI GAGTC 4 cut(s) 182, 863, 1139, 1366
SduI GDGCHC 3 cut(s) 15, 606, 1455
SfaNI GCATC 3 cut(s) 582, 925, 1071
SfcI CTRYAG 1 cut(s) 55
Sfr274I CTCGAG 1 cut(s) 653
SinI GGWCC 1 cut(s) 133
SlaI CTCGAG 1 cut(s) 653
SmiMI CAYNNNNRTG 1 cut(s) 1448
SmlI CTYRAG 4 cut(s) 501, 653, 773, 1338
SmoI CTYRAG 4 cut(s) 501, 653, 773, 1338
SpeI ACTAGT 1 cut(s) 1157
Sse9I AATT 7 cut(s) 219, 248, 620, 862, 949, 1025, 1132
SseBI AGGCCT 2 cut(s) 507, 1004
SsiI CCGC 2 cut(s) 783, 810
SspMI CTAG 4 cut(s) 446, 561, 1049, 1158
SstI GAGCTC 2 cut(s) 15, 606
StuI AGGCCT 2 cut(s) 507, 1004
TaaI ACNGT 2 cut(s) 56, 793
TaiI ACGT 1 cut(s) 494
TaqI TCGA 3 cut(s) 296, 654, 921
TasI AATT 7 cut(s) 219, 248, 620, 862, 949, 1025, 1132
TatI WGTACW 2 cut(s) 203, 1051
TauI GCSGC 1 cut(s) 812
TfiI GAWTC 5 cut(s) 71, 302, 325, 649, 958
Tru1I TTAA 8 cut(s) 356, 525, 618, 840, 1154, 1187, 1319, 1457
Tru9I TTAA 8 cut(s) 356, 525, 618, 840, 1154, 1187, 1319, 1457
TscAI CASTG 1 cut(s) 722
TseFI GTSAC 1 cut(s) 787
TseI GCWGC 3 cut(s) 351, 759, 1384
Tsp45I GTSAC 1 cut(s) 787
TspDTI ATGAA 4 cut(s) 500, 573, 671, 714
TspRI CASTG 1 cut(s) 722
VpaK11BI GGWCC 1 cut(s) 133
XagI CCTNNNNNAGG 1 cut(s) 317
XapI RAATTY 2 cut(s) 248, 620
XbaI TCTAGA 1 cut(s) 560
XceI RCATGY 2 cut(s) 976, 1251
XhoI CTCGAG 1 cut(s) 653
XmnI GAANNNNTTC 2 cut(s) 1004, 1301
XspI CTAG 4 cut(s) 446, 561, 1049, 1158
ZrmI AGTACT 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.