Rmu_sc0001817.1_g000001

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001817.1
OG0015192
Physical Location & Seq
Reverse (-)
1 .. 1915
1915 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 501 bp
atgcctctacaagaagaagaagatggagatgtatcgaccctaaaccaaacccagaacccgaatcgagtgataaaccaaaacatgtttgatggaagagaagaagatgaaacaataacaacccagaacatgaatctggagattccggaggatatcatcaccgacatcctgaaacggctgccggcgaagtctctggttcgattcaggtgcatgtgcaagccatggggctctctgctcacagatcctgccttcatcaaatctcatctggagcgaaaccgatccctggattgggcctttgaaggattgcctatgcacagcgagaactcggagttgttgttgttgttgaacaccgggattgggattagatggtttcagtggagaagcttgagaattgaagggcctcatcaaaaagcccgtggatcaagtgggccgatggaggcccgctggcccgcagggcttttggccaggcccggccagtcaaaaaaccagcaaaagcccgccttg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

167

Amino Acids

19.1

Weight (kDa)

5.95

Isoelectric Point (pI)

58.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 45 - 83 3.4e-09 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 142
AciI CCGC 3 cut(s) 439, 447, 495
AclWI GGATC 3 cut(s) 233, 270, 424
AcoI YGGCCR 2 cut(s) 459, 469
AfiI CCNNNNNNNGG 4 cut(s) 280, 285, 286, 354
AflIII ACRYGT 1 cut(s) 81
AgsI TTSAA 3 cut(s) 296, 343, 392
AjnI CCWGG 2 cut(s) 279, 461
AluBI AGCT 1 cut(s) 381
AluI AGCT 1 cut(s) 381
Alw26I GTCTC 1 cut(s) 192
AlwI GGATC 3 cut(s) 233, 270, 424
AlwNI CAGNNNCTG 1 cut(s) 242
Aor13HI TCCGGA 1 cut(s) 142
AoxI GGCC 8 cut(s) 288, 395, 425, 435, 443, 459, 464, 469
ApeKI GCWGC 1 cut(s) 175
AspS9I GGNCC 6 cut(s) 288, 395, 425, 436, 444, 465
AsuC2I CCSGG 2 cut(s) 349, 468
AsuHPI GGTGA 1 cut(s) 148
BalI TGGCCA 1 cut(s) 461
BanII GRGCYC 1 cut(s) 227
BbvI GCAGC 1 cut(s) 162
BccI CCATC 4 cut(s) 17, 83, 357, 424
BceAI ACGGC 1 cut(s) 188
BcgI CGANNNNNNTGC 2 cut(s) 186, 220
BciT130I CCWGG 2 cut(s) 281, 463
BcnI CCSGG 2 cut(s) 349, 468
BcoDI GTCTC 1 cut(s) 192
BglI GCCNNNNNGGC 1 cut(s) 451
BisI GCNGC 1 cut(s) 176
BlsI GCNGC 1 cut(s) 177
Bme1390I CCNGG 4 cut(s) 281, 349, 463, 468
BmgT120I GGNCC 6 cut(s) 288, 395, 425, 436, 444, 465
BmrFI CCNGG 4 cut(s) 281, 349, 463, 468
BpmI CTGGAG 2 cut(s) 155, 284
BpuEI CTTGAG 1 cut(s) 403
BpuMI CCSGG 2 cut(s) 349, 468
BsaJI CCNNGG 3 cut(s) 218, 279, 412
BsaWI WCCGGW 1 cut(s) 142
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
Bsc4I CCNNNNNNNGG 4 cut(s) 280, 285, 286, 354
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 1 cut(s) 472
BseAI TCCGGA 1 cut(s) 142
BseBI CCWGG 2 cut(s) 281, 463
BseDI CCNNGG 3 cut(s) 218, 279, 412
BseGI GGATG 1 cut(s) 162
BseLI CCNNNNNNNGG 4 cut(s) 280, 285, 286, 354
BseNI ACTGG 1 cut(s) 472
BseXI GCAGC 1 cut(s) 162
BshFI GGCC 8 cut(s) 290, 397, 427, 437, 445, 461, 466, 471
BsiSI CCGG 4 cut(s) 143, 179, 348, 468
BslI CCNNNNNNNGG 4 cut(s) 280, 285, 286, 354
BsmAI GTCTC 1 cut(s) 192
BsnI GGCC 8 cut(s) 290, 397, 427, 437, 445, 461, 466, 471
Bsp1286I GDGCHC 1 cut(s) 227
Bsp13I TCCGGA 1 cut(s) 142
Bsp143I GATC 3 cut(s) 238, 275, 416
Bsp19I CCATGG 1 cut(s) 218
BspACI CCGC 3 cut(s) 439, 447, 495
BspANI GGCC 8 cut(s) 290, 397, 427, 437, 445, 461, 466, 471
BspEI TCCGGA 1 cut(s) 142
BspPI GGATC 3 cut(s) 233, 270, 424
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 1 cut(s) 472
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 3 cut(s) 218, 279, 412
BssMI GATC 3 cut(s) 238, 275, 416
BssT1I CCWWGG 1 cut(s) 218
Bst2UI CCWGG 2 cut(s) 281, 463
Bst6I CTCTTC 1 cut(s) 88
BstC8I GCNNGC 6 cut(s) 180, 215, 439, 443, 447, 495
BstDSI CCRYGG 2 cut(s) 218, 412
BstF5I GGATG 1 cut(s) 162
BstKTI GATC 3 cut(s) 241, 278, 419
BstMAI GTCTC 1 cut(s) 192
BstMBI GATC 3 cut(s) 238, 275, 416
BstMWI GCNNNNNNNGC 1 cut(s) 451
BstNI CCWGG 2 cut(s) 281, 463
BstNSI RCATGY 2 cut(s) 85, 211
BstSCI CCNGG 4 cut(s) 279, 347, 461, 466
BstV1I GCAGC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BsuRI GGCC 8 cut(s) 290, 397, 427, 437, 445, 461, 466, 471
BtgI CCRYGG 2 cut(s) 218, 412
BtsCI GGATG 1 cut(s) 162
BtsIMutI CAGTG 1 cut(s) 377
Cac8I GCNNGC 6 cut(s) 180, 215, 439, 443, 447, 495
CaiI CAGNNNCTG 1 cut(s) 242
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 6 cut(s) 288, 395, 425, 436, 444, 465
CviAII CATG 4 cut(s) 82, 127, 208, 219
DpnI GATC 3 cut(s) 240, 277, 418
DpnII GATC 3 cut(s) 238, 275, 416
EaeI YGGCCR 2 cut(s) 459, 469
Eam1104I CTCTTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 218
Eco24I GRGCYC 1 cut(s) 227
Eco32I GATATC 1 cut(s) 151
EcoO109I RGGNCCY 1 cut(s) 395
EcoRII CCWGG 2 cut(s) 279, 461
EcoRV GATATC 1 cut(s) 151
EcoT14I CCWWGG 1 cut(s) 218
EcoT38I GRGCYC 1 cut(s) 227
ErhI CCWWGG 1 cut(s) 218
FaeI CATG 4 cut(s) 85, 130, 211, 222
FaiI YATR 5 cut(s) 83, 128, 209, 220, 308
FalI AAGNNNNNCTT 1 cut(s) 482
FatI CATG 4 cut(s) 81, 126, 207, 218
FauI CCCGC 2 cut(s) 446, 454
Fnu4HI GCNGC 1 cut(s) 176
FokI GGATG 1 cut(s) 149
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 1 cut(s) 176
GluI GCNGC 1 cut(s) 176
GsuI CTGGAG 2 cut(s) 155, 284
HaeIII GGCC 8 cut(s) 290, 397, 427, 437, 445, 461, 466, 471
HapII CCGG 4 cut(s) 143, 179, 348, 468
Hin1II CATG 4 cut(s) 85, 130, 211, 222
HindIII AAGCTT 1 cut(s) 379
HinfI GANTC 4 cut(s) 61, 130, 139, 198
HpaII CCGG 4 cut(s) 143, 179, 348, 468
HphI GGTGA 1 cut(s) 148
Hpy188I TCNGA 1 cut(s) 325
Hpy188III TCNNGA 4 cut(s) 134, 143, 166, 263
HpyAV CCTTC 3 cut(s) 256, 290, 386
HpyCH4V TGCA 3 cut(s) 207, 213, 310
HpyF10VI GCNNNNNNNGC 1 cut(s) 451
Hsp92II CATG 4 cut(s) 85, 130, 211, 222
Kpn2I TCCGGA 1 cut(s) 142
KroI GCCGGC 1 cut(s) 178
KroNI GCCGGC 1 cut(s) 180
Kzo9I GATC 3 cut(s) 238, 275, 416
LmnI GCTCC 1 cut(s) 265
Lsp1109I GCAGC 1 cut(s) 162
MalI GATC 3 cut(s) 240, 277, 418
MboI GATC 3 cut(s) 238, 275, 416
MboII GAAGA 6 cut(s) 26, 29, 32, 105, 110, 113
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 227
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 1 cut(s) 387
MluNI TGGCCA 1 cut(s) 461
MnlI CCTC 4 cut(s) 15, 139, 408, 427
Mox20I TGGCCA 1 cut(s) 461
MroI TCCGGA 1 cut(s) 142
MroNI GCCGGC 1 cut(s) 178
MscI TGGCCA 1 cut(s) 461
Msp20I TGGCCA 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 441
MspI CCGG 4 cut(s) 143, 179, 348, 468
MspR9I CCNGG 4 cut(s) 281, 349, 463, 468
MvaI CCWGG 2 cut(s) 281, 463
MwoI GCNNNNNNNGC 1 cut(s) 451
NaeI GCCGGC 1 cut(s) 180
NciI CCSGG 2 cut(s) 349, 468
NcoI CCATGG 1 cut(s) 218
NdeII GATC 3 cut(s) 238, 275, 416
NgoMIV GCCGGC 1 cut(s) 178
NlaIII CATG 4 cut(s) 85, 130, 211, 222
NspI RCATGY 2 cut(s) 85, 211
PciI ACATGT 1 cut(s) 81
PdiI GCCGGC 1 cut(s) 180
PfeI GAWTC 4 cut(s) 61, 130, 139, 198
PkrI GCNGC 1 cut(s) 177
PscI ACATGT 1 cut(s) 81
Psp6I CCWGG 2 cut(s) 279, 461
PspGI CCWGG 2 cut(s) 279, 461
PspPI GGNCC 6 cut(s) 288, 395, 425, 436, 444, 465
PstNI CAGNNNCTG 1 cut(s) 242
PsuI RGATCY 1 cut(s) 238
SatI GCNGC 1 cut(s) 176
Sau3AI GATC 3 cut(s) 238, 275, 416
Sau96I GGNCC 6 cut(s) 288, 395, 425, 436, 444, 465
ScrFI CCNGG 4 cut(s) 281, 349, 463, 468
SduI GDGCHC 1 cut(s) 227
SetI ASST 2 cut(s) 206, 383
SmlI CTYRAG 1 cut(s) 382
SmoI CTYRAG 1 cut(s) 382
Sse9I AATT 1 cut(s) 387
SsiI CCGC 3 cut(s) 439, 447, 495
StyD4I CCNGG 4 cut(s) 279, 347, 461, 466
StyI CCWWGG 1 cut(s) 218
TaqI TCGA 3 cut(s) 35, 64, 196
TasI AATT 1 cut(s) 387
TfiI GAWTC 4 cut(s) 61, 130, 139, 198
TscAI CASTG 1 cut(s) 377
TseI GCWGC 1 cut(s) 175
TspDTI ATGAA 3 cut(s) 120, 143, 238
TspRI CASTG 1 cut(s) 377
XceI RCATGY 2 cut(s) 85, 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.