Rmu_sc0001849.1_g000011

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001849.1
OG0005691
Physical Location & Seq
Reverse (-)
48211 .. 51760
3550 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
atgaacgaacgagataagagagataaacgattgggccggggcggaccttgcgaagaagagaaggcgatcggagaagagagcgcgagggcggtggggctcagatctgggatcggtggtggttctcaggcggtgtggttgggtcgtggcctgacacgtgttcaggatctgtcggcagtcccagactatggctttgggttacgtattcaatttgctattgatttgtctccacgtgacacggcaccgtttcggttcaacccaaaacgcagcgttttcggttcagcccaaaacgcggcgtttcggtctctttggcagcataaacaccctgctccttgcaattctgtctctatcgcttttacaaactcagcagtctggttatgtttttgggagtgtccaagaatgagtcttatgaagcggtatgtgctgcggttgttcatatcattgaagtacatcacagcaaacgtcgttgacagaaataatggccggattgttgtaacagcatctacaattgaacattcagttaagagctcgctcgagtgtggccgctcttgcaatgcaaaggcggcagcagttgttggagaggtgctcgctatgcgacttaaggtggaaggtctagaccagggacagggacgagggattcatgtcaatgtaaataatgaagttgagaagaagggttttaagaaccgtaccaaggtatgggctattgtcaatgctcttaaaaacaatggagttaaactcattcttgatgataatgatgaaaatagttctcagccaagttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

258

Amino Acids

28.43

Weight (kDa)

9.75

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 137 - 206 2.8e-07 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 238
AccB7I CCANNNNNTGG 2 cut(s) 185, 693
AccBSI CCGCTC 1 cut(s) 543
AccII CGCG 2 cut(s) 83, 290
AciI CCGC 8 cut(s) 42, 89, 128, 290, 412, 424, 541, 560
AclWI GGATC 2 cut(s) 116, 171
AcoI YGGCCR 2 cut(s) 478, 538
AcvI CACGTG 2 cut(s) 155, 230
AfaI GTAC 2 cut(s) 446, 685
AfiI CCNNNNNNNGG 5 cut(s) 185, 289, 622, 688, 693
AflII CTTAAG 1 cut(s) 596
AflIII ACRYGT 2 cut(s) 152, 154
AgsI TTSAA 4 cut(s) 206, 253, 442, 509
AjnI CCWGG 1 cut(s) 615
AluBI AGCT 1 cut(s) 525
AluI AGCT 1 cut(s) 525
Alw21I GWGCWC 2 cut(s) 527, 585
Alw26I GTCTC 3 cut(s) 228, 306, 346
AlwI GGATC 2 cut(s) 116, 171
AlwNI CAGNNNCTG 1 cut(s) 166
Ama87I CYCGRG 1 cut(s) 530
AoxI GGCC 4 cut(s) 34, 145, 478, 538
ApeKI GCWGC 4 cut(s) 264, 310, 421, 563
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 2 cut(s) 34, 44
AsuC2I CCSGG 1 cut(s) 38
AvaI CYCGRG 1 cut(s) 530
AvaII GGWCC 1 cut(s) 44
BanI GGYRCC 1 cut(s) 238
BanII GRGCYC 2 cut(s) 99, 527
BbrPI CACGTG 2 cut(s) 155, 230
Bbv12I GWGCWC 2 cut(s) 527, 585
BbvI GCAGC 4 cut(s) 276, 322, 408, 575
BceAI ACGGC 1 cut(s) 252
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BciT130I CCWGG 1 cut(s) 617
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 3 cut(s) 228, 306, 346
BfaI CTAG 1 cut(s) 611
BfrI CTTAAG 1 cut(s) 596
BglII AGATCT 1 cut(s) 101
BisI GCNGC 7 cut(s) 265, 291, 311, 422, 541, 561, 564
BlsI GCNGC 7 cut(s) 266, 292, 312, 423, 542, 562, 565
Bme1390I CCNGG 2 cut(s) 38, 617
Bme18I GGWCC 1 cut(s) 44
BmeT110I CYCGRG 1 cut(s) 530
BmgT120I GGNCC 2 cut(s) 34, 44
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 2 cut(s) 38, 617
BmsI GCATC 1 cut(s) 506
BplI GAGNNNNNCTC 4 cut(s) 567, 599, 717, 749
BpuMI CCSGG 1 cut(s) 38
BsaAI YACGTR 3 cut(s) 155, 200, 230
BsaI GGTCTC 1 cut(s) 306
BsaJI CCNNGG 3 cut(s) 37, 616, 687
Bsc4I CCNNNNNNNGG 5 cut(s) 185, 289, 622, 688, 693
Bse3DI GCAATG 1 cut(s) 556
BseBI CCWGG 1 cut(s) 617
BseDI CCNNGG 3 cut(s) 37, 616, 687
BseLI CCNNNNNNNGG 5 cut(s) 185, 289, 622, 688, 693
BseMI GCAATG 1 cut(s) 556
BseMII CTCAG 3 cut(s) 112, 137, 375
BseXI GCAGC 4 cut(s) 276, 322, 408, 575
Bsh1236I CGCG 2 cut(s) 83, 290
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 4 cut(s) 36, 147, 480, 540
BshNI GGYRCC 1 cut(s) 238
BsiEI CGRYCG 1 cut(s) 69
BsiHKAI GWGCWC 2 cut(s) 527, 585
BsiHKCI CYCGRG 1 cut(s) 530
BsiSI CCGG 2 cut(s) 37, 481
BslFI GGGAC 3 cut(s) 161, 633, 639
BslI CCNNNNNNNGG 5 cut(s) 185, 289, 622, 688, 693
BsmAI GTCTC 3 cut(s) 228, 306, 346
BsmFI GGGAC 3 cut(s) 161, 633, 639
BsnI GGCC 4 cut(s) 36, 147, 480, 540
Bso31I GGTCTC 1 cut(s) 306
BsoBI CYCGRG 1 cut(s) 530
Bsp1286I GDGCHC 3 cut(s) 99, 527, 585
Bsp143I GATC 4 cut(s) 66, 101, 108, 163
BspACI CCGC 8 cut(s) 42, 89, 128, 290, 412, 424, 541, 560
BspANI GGCC 4 cut(s) 36, 147, 480, 540
BspCNI CTCAG 3 cut(s) 111, 136, 374
BspFNI CGCG 2 cut(s) 83, 290
BspLI GGNNCC 1 cut(s) 240
BspPI GGATC 2 cut(s) 116, 171
BspT107I GGYRCC 1 cut(s) 238
BspTI CTTAAG 1 cut(s) 596
BspTNI GGTCTC 1 cut(s) 306
BsrBI CCGCTC 1 cut(s) 543
BsrDI GCAATG 1 cut(s) 556
BssECI CCNNGG 3 cut(s) 37, 616, 687
BssMI GATC 4 cut(s) 66, 101, 108, 163
BssT1I CCWWGG 1 cut(s) 687
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 2 cut(s) 243, 683
Bst6I CTCTTC 2 cut(s) 51, 69
BstAFI CTTAAG 1 cut(s) 596
BstBAI YACGTR 3 cut(s) 155, 200, 230
BstC8I GCNNGC 2 cut(s) 527, 585
BstDEI CTNAG 4 cut(s) 98, 123, 361, 765
BstFNI CGCG 2 cut(s) 83, 290
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 4 cut(s) 69, 104, 111, 166
BstMAI GTCTC 3 cut(s) 228, 306, 346
BstMBI GATC 4 cut(s) 66, 101, 108, 163
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 6 cut(s) 48, 287, 418, 546, 560, 589
BstNI CCWGG 1 cut(s) 617
BstSCI CCNGG 2 cut(s) 36, 615
BstSNI TACGTA 1 cut(s) 200
BstUI CGCG 2 cut(s) 83, 290
BstV1I GCAGC 4 cut(s) 276, 322, 408, 575
BstX2I RGATCY 2 cut(s) 101, 163
BstYI RGATCY 2 cut(s) 101, 163
BsuRI GGCC 4 cut(s) 36, 147, 480, 540
Cac8I GCNNGC 2 cut(s) 527, 585
CaiI CAGNNNCTG 1 cut(s) 166
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 2 cut(s) 34, 44
Csp6I GTAC 2 cut(s) 445, 684
CviAII CATG 1 cut(s) 638
CviQI GTAC 2 cut(s) 445, 684
DdeI CTNAG 4 cut(s) 98, 123, 361, 765
DpnI GATC 4 cut(s) 68, 103, 110, 165
DpnII GATC 4 cut(s) 66, 101, 108, 163
EaeI YGGCCR 2 cut(s) 478, 538
Eam1104I CTCTTC 2 cut(s) 51, 69
EarI CTCTTC 2 cut(s) 51, 69
EciI GGCGGA 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 525
Eco105I TACGTA 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 687
Eco24I GRGCYC 2 cut(s) 99, 527
Eco31I GGTCTC 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 44
Eco53kI GAGCTC 1 cut(s) 525
Eco72I CACGTG 2 cut(s) 155, 230
Eco88I CYCGRG 1 cut(s) 530
EcoICRI GAGCTC 1 cut(s) 525
EcoRII CCWGG 1 cut(s) 615
EcoT14I CCWWGG 1 cut(s) 687
EcoT38I GRGCYC 2 cut(s) 99, 527
ErhI CCWWGG 1 cut(s) 687
FaeI CATG 1 cut(s) 641
FaiI YATR 9 cut(s) 186, 315, 376, 407, 417, 434, 590, 639, 694
FaqI GGGAC 3 cut(s) 161, 633, 639
FatI CATG 1 cut(s) 637
Fnu4HI GCNGC 7 cut(s) 265, 291, 311, 422, 541, 561, 564
FriOI GRGCYC 2 cut(s) 99, 527
Fsp4HI GCNGC 7 cut(s) 265, 291, 311, 422, 541, 561, 564
FspBI CTAG 1 cut(s) 611
GlaI GCGC 1 cut(s) 82
GluI GCNGC 7 cut(s) 265, 291, 311, 422, 541, 561, 564
HaeIII GGCC 4 cut(s) 36, 147, 480, 540
HapII CCGG 2 cut(s) 37, 481
HhaI GCGC 1 cut(s) 83
Hin1II CATG 1 cut(s) 641
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HinfI GANTC 2 cut(s) 400, 634
HpaII CCGG 2 cut(s) 37, 481
Hpy166II GTNNAC 1 cut(s) 466
Hpy188I TCNGA 2 cut(s) 71, 101
Hpy188III TCNNGA 3 cut(s) 161, 611, 740
Hpy8I GTNNAC 1 cut(s) 466
Hpy99I CGWCG 1 cut(s) 464
HpyAV CCTTC 3 cut(s) 55, 599, 661
HpyCH4III ACNGT 2 cut(s) 243, 683
HpyCH4IV ACGT 4 cut(s) 154, 199, 229, 459
HpyCH4V TGCA 3 cut(s) 333, 549, 554
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 287, 418, 546, 560, 589
HpyF3I CTNAG 4 cut(s) 98, 123, 361, 765
HpySE526I ACGT 4 cut(s) 154, 199, 229, 459
Hsp92II CATG 1 cut(s) 641
HspAI GCGC 1 cut(s) 81
Kzo9I GATC 4 cut(s) 66, 101, 108, 163
LmnI GCTCC 1 cut(s) 331
Lsp1109I GCAGC 4 cut(s) 276, 322, 408, 575
LweI GCATC 1 cut(s) 506
MaeI CTAG 1 cut(s) 611
MaeII ACGT 4 cut(s) 154, 199, 229, 459
MaeIII GTNAC 3 cut(s) 195, 230, 490
MalI GATC 4 cut(s) 68, 103, 110, 165
MbiI CCGCTC 1 cut(s) 543
MboI GATC 4 cut(s) 66, 101, 108, 163
MboII GAAGA 4 cut(s) 65, 68, 86, 676
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 2 cut(s) 101, 163
MhlI GDGCHC 3 cut(s) 99, 527, 585
MluCI AATT 3 cut(s) 206, 334, 504
MlyI GAGTC 1 cut(s) 409
MmeI TCCRAC 1 cut(s) 553
MnlI CCTC 3 cut(s) 78, 571, 623
MseI TTAA 6 cut(s) 519, 597, 675, 714, 729, 775
MslI CAYNNNNRTG 1 cut(s) 642
MspCI CTTAAG 1 cut(s) 596
MspI CCGG 2 cut(s) 37, 481
MspR9I CCNGG 2 cut(s) 38, 617
MunI CAATTG 1 cut(s) 504
MvaI CCWGG 1 cut(s) 617
MvnI CGCG 2 cut(s) 83, 290
MwoI GCNNNNNNNGC 6 cut(s) 48, 287, 418, 546, 560, 589
NciI CCSGG 1 cut(s) 38
NdeII GATC 4 cut(s) 66, 101, 108, 163
NlaIII CATG 1 cut(s) 641
NlaIV GGNNCC 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 230
PaeR7I CTCGAG 1 cut(s) 530
PfeI GAWTC 1 cut(s) 634
PflMI CCANNNNNTGG 2 cut(s) 185, 693
PkrI GCNGC 7 cut(s) 266, 292, 312, 423, 542, 562, 565
Ple19I CGATCG 1 cut(s) 69
PleI GAGTC 1 cut(s) 408
PmaCI CACGTG 2 cut(s) 155, 230
PmlI CACGTG 2 cut(s) 155, 230
PpsI GAGTC 1 cut(s) 408
Ppu21I YACGTR 3 cut(s) 155, 200, 230
Psp124BI GAGCTC 1 cut(s) 527
Psp6I CCWGG 1 cut(s) 615
PspCI CACGTG 2 cut(s) 155, 230
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 2 cut(s) 34, 44
PspXI VCTCGAGB 1 cut(s) 530
PstNI CAGNNNCTG 1 cut(s) 166
PsuI RGATCY 2 cut(s) 101, 163
PvuI CGATCG 1 cut(s) 69
RsaI GTAC 2 cut(s) 446, 685
RsaNI GTAC 2 cut(s) 445, 684
RseI CAYNNNNRTG 1 cut(s) 642
SacI GAGCTC 1 cut(s) 527
SaqAI TTAA 6 cut(s) 519, 597, 675, 714, 729, 775
SatI GCNGC 7 cut(s) 265, 291, 311, 422, 541, 561, 564
Sau3AI GATC 4 cut(s) 66, 101, 108, 163
Sau96I GGNCC 2 cut(s) 34, 44
SchI GAGTC 1 cut(s) 409
ScrFI CCNGG 2 cut(s) 38, 617
SduI GDGCHC 3 cut(s) 99, 527, 585
SfaNI GCATC 1 cut(s) 506
Sfr274I CTCGAG 1 cut(s) 530
SinI GGWCC 1 cut(s) 44
SlaI CTCGAG 1 cut(s) 530
SmiMI CAYNNNNRTG 1 cut(s) 642
SmlI CTYRAG 2 cut(s) 530, 596
SmoI CTYRAG 2 cut(s) 530, 596
SnaBI TACGTA 1 cut(s) 200
Sse9I AATT 3 cut(s) 206, 334, 504
SsiI CCGC 8 cut(s) 42, 89, 128, 290, 412, 424, 541, 560
SspMI CTAG 1 cut(s) 611
SstI GAGCTC 1 cut(s) 527
StyD4I CCNGG 2 cut(s) 36, 615
StyI CCWWGG 1 cut(s) 687
TaaI ACNGT 2 cut(s) 243, 683
TaiI ACGT 4 cut(s) 157, 202, 232, 462
TaqI TCGA 1 cut(s) 531
TaqII GACCGA 1 cut(s) 288
TasI AATT 3 cut(s) 206, 334, 504
TatI WGTACW 1 cut(s) 444
TauI GCSGC 3 cut(s) 293, 543, 563
TfiI GAWTC 1 cut(s) 634
Tru1I TTAA 6 cut(s) 519, 597, 675, 714, 729, 775
Tru9I TTAA 6 cut(s) 519, 597, 675, 714, 729, 775
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 4 cut(s) 264, 310, 421, 563
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 6 cut(s) 17, 421, 422, 626, 669, 768
Van91I CCANNNNNTGG 2 cut(s) 185, 693
Vha464I CTTAAG 1 cut(s) 596
VpaK11BI GGWCC 1 cut(s) 44
XbaI TCTAGA 1 cut(s) 610
XhoI CTCGAG 1 cut(s) 530
XspI CTAG 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.