Rmu_sc0001955.1_g000002

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001955.1
OG0006200
Physical Location & Seq
Forward (+)
3594 .. 4841
1248 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1248 bp
atggaatggatggagtttaggaaaattcattatgctttgcctgactatgccgtacggttggatcttacggcgaaagccaaagggatagaggcagctgagagctacttcagcaatttagcaccgtctgcgaagaacaaattgacttacggatcccttttgaactgttattgcaaggaagtgatggaagagaaggcattggcgctttatcagaagatggatgagttggaccatgctaactctactctggtttttaacaatctcatgtctttgtatatgagaaaggagcggccggagaaagtggcgcctttcgtagagcaaatgaagcagagggagatatgtttagatacgtttagttacaatatctggatgcagagctatgcctctttgaatgatattgaaggcgtggagagagttgtggaagagatgcaaagccaagatcaagacaaatgcaactggtcaacatatagtaatctggcttccatctatgtaaaggcgcagctttttgagaaggctgagctagcgcttaaaatgtcggagaaaatgatgataccgctgaagcagcgccagacataccatttcttgattaccctctacgccaacactggtaatctaggtgaggtcaaaagaatatgggaatccctcaagttagctttcccagaaaccaccaatatgagctatatacttatgcttcaagctctatgtaagctaaaagatattgagggtttaaaagaatgctttgaggagtggcagtccaattgttccagttatgatatgaggttagcaaatgtagttatacgtgcttatcttagtcaggacatgtatgaagaagcgctgttgatctttaaggatgctactaagaggtctaaagggcctttcttcaaggctcgagaaatgttcatggtttacttcttggatgcccgtcaggttgattcggccattagctatttgggagaggcaatttcagaaaccaaggatgagaattggcatccgtctccagaaatcattggtgcattttttaagtattttgaggaaacaaaagatgttgatagctctgaaaactttcataagattctgaagcgtctaaattgtctcagttccaatgagtattctttgttgcttaaggtttatctagctgctggtgaattgcttcctgagatatgtcagagattgaaggaagacaacattcaaataagccccgagcttgaggaattggtggagagggtttccccaagtagatcgacagcatag

Protein Analysis

415

Amino Acids

48.3

Weight (kDa)

5.21

Isoelectric Point (pI)

56.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 81 - 127 9.9e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 301
AccBSI CCGCTC 1 cut(s) 286
AciI CCGC 2 cut(s) 286, 551
AclWI GGATC 3 cut(s) 69, 144, 157
AcoI YGGCCR 2 cut(s) 287, 933
AcsI RAATTY 1 cut(s) 24
AcuI CTGAAG 3 cut(s) 91, 575, 1094
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 1 cut(s) 54
AfeI AGCGCT 2 cut(s) 522, 831
AflII CTTAAG 1 cut(s) 1118
AflIII ACRYGT 1 cut(s) 816
AgsI TTSAA 7 cut(s) 160, 388, 398, 692, 880, 1171, 1187
Alw26I GTCTC 2 cut(s) 996, 1094
AlwI GGATC 3 cut(s) 69, 144, 157
Ama87I CYCGRG 2 cut(s) 885, 1196
Aor51HI AGCGCT 2 cut(s) 522, 831
AoxI GGCC 3 cut(s) 287, 869, 933
ApeKI GCWGC 4 cut(s) 92, 496, 559, 1133
ApoI RAATTY 1 cut(s) 24
Asp700I GAANNNNTTC 2 cut(s) 1059, 1146
AspLEI GCGC 6 cut(s) 202, 304, 496, 523, 564, 832
AspS9I GGNCC 2 cut(s) 226, 869
AsuHPI GGTGA 2 cut(s) 626, 1151
AsuNHI GCTAGC 1 cut(s) 517
AvaI CYCGRG 2 cut(s) 885, 1196
AvaII GGWCC 1 cut(s) 226
BamHI GGATCC 1 cut(s) 149
BanI GGYRCC 1 cut(s) 301
BarI GAAGNNNNNNTAC 2 cut(s) 460, 492
BbsI GAAGAC 1 cut(s) 1182
BbvI GCAGC 4 cut(s) 104, 508, 571, 1120
BccI CCATC 4 cut(s) 4, 175, 208, 488
BceAI ACGGC 2 cut(s) 35, 84
BcoDI GTCTC 2 cut(s) 996, 1094
BfaI CTAG 3 cut(s) 518, 611, 1130
BfoI RGCGCY 5 cut(s) 203, 305, 524, 565, 833
BfrI CTTAAG 1 cut(s) 1118
BisI GCNGC 5 cut(s) 93, 287, 497, 560, 1134
BlpI GCTNAGC 1 cut(s) 513
BlsI GCNGC 5 cut(s) 94, 288, 498, 561, 1135
Bme18I GGWCC 1 cut(s) 226
BmeT110I CYCGRG 2 cut(s) 885, 1196
BmgT120I GGNCC 2 cut(s) 226, 869
BmiI GGNNCC 2 cut(s) 151, 303
BmsI GCATC 5 cut(s) 357, 414, 838, 904, 994
BmtI GCTAGC 1 cut(s) 521
BpiI GAAGAC 1 cut(s) 1182
BpmI CTGGAG 1 cut(s) 978
Bpu1102I GCTNAGC 1 cut(s) 513
BpuEI CTTGAG 2 cut(s) 626, 1223
BsaAI YACGTR 1 cut(s) 797
BsaHI GRCGYC 1 cut(s) 302
BsaJI CCNNGG 1 cut(s) 969
BsaXI ACNNNNNCTCC 2 cut(s) 734, 764
Bse1I ACTGG 3 cut(s) 458, 607, 762
BseDI CCNNGG 1 cut(s) 969
BseGI GGATG 7 cut(s) 15, 223, 372, 853, 919, 979, 985
BseMII CTCAG 4 cut(s) 87, 504, 1105, 1143
BseNI ACTGG 3 cut(s) 458, 607, 762
BseRI GAGGAG 1 cut(s) 755
BseX3I CGGCCG 1 cut(s) 287
BseXI GCAGC 4 cut(s) 104, 508, 571, 1120
Bsh1285I CGRYCG 1 cut(s) 290
BshFI GGCC 3 cut(s) 289, 871, 935
BshNI GGYRCC 1 cut(s) 301
BsiEI CGRYCG 1 cut(s) 290
BsiHKCI CYCGRG 2 cut(s) 885, 1196
BsiSI CCGG 1 cut(s) 290
BsiWI CGTACG 1 cut(s) 52
BsmAI GTCTC 2 cut(s) 996, 1094
BsmBI CGTCTC 1 cut(s) 996
BsmI GAATGC 1 cut(s) 737
BsnI GGCC 3 cut(s) 289, 871, 935
BsoBI CYCGRG 2 cut(s) 885, 1196
Bsp143I GATC 5 cut(s) 61, 149, 436, 837, 1235
Bsp1720I GCTNAGC 1 cut(s) 513
BspACI CCGC 2 cut(s) 286, 551
BspANI GGCC 3 cut(s) 289, 871, 935
BspCNI CTCAG 4 cut(s) 88, 505, 1104, 1144
BspLI GGNNCC 2 cut(s) 151, 303
BspOI GCTAGC 1 cut(s) 521
BspPI GGATC 3 cut(s) 69, 144, 157
BspT107I GGYRCC 1 cut(s) 301
BspTI CTTAAG 1 cut(s) 1118
BsrBI CCGCTC 1 cut(s) 286
BsrI ACTGG 3 cut(s) 458, 607, 762
BssECI CCNNGG 1 cut(s) 969
BssMI GATC 5 cut(s) 61, 149, 436, 837, 1235
BssNI GRCGYC 1 cut(s) 302
BssT1I CCWWGG 1 cut(s) 969
Bst4CI ACNGT 3 cut(s) 57, 123, 164
Bst6I CTCTTC 2 cut(s) 180, 414
BstACI GRCGYC 1 cut(s) 302
BstAFI CTTAAG 1 cut(s) 1118
BstAPI GCANNNNNTGC 1 cut(s) 125
BstBAI YACGTR 1 cut(s) 797
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 6 cut(s) 96, 513, 806, 855, 1091, 1152
BstF5I GGATG 7 cut(s) 15, 223, 372, 853, 919, 979, 985
BstH2I RGCGCY 5 cut(s) 203, 305, 524, 565, 833
BstHHI GCGC 6 cut(s) 202, 304, 496, 523, 564, 832
BstKTI GATC 5 cut(s) 64, 152, 439, 840, 1238
BstMAI GTCTC 2 cut(s) 996, 1094
BstMBI GATC 5 cut(s) 61, 149, 436, 837, 1235
BstMCI CGRYCG 1 cut(s) 290
BstMWI GCNNNNNNNGC 5 cut(s) 108, 125, 322, 518, 559
BstNSI RCATGY 1 cut(s) 820
BstV1I GCAGC 4 cut(s) 104, 508, 571, 1120
BstV2I GAAGAC 1 cut(s) 1182
BstX2I RGATCY 2 cut(s) 61, 149
BstYI RGATCY 2 cut(s) 61, 149
BstZI CGGCCG 1 cut(s) 287
BsuRI GGCC 3 cut(s) 289, 871, 935
BtsCI GGATG 7 cut(s) 15, 223, 372, 853, 919, 979, 985
BtsIMutI CAGTG 1 cut(s) 600
Cac8I GCNNGC 1 cut(s) 519
CfoI GCGC 6 cut(s) 202, 304, 496, 523, 564, 832
Cfr13I GGNCC 2 cut(s) 226, 869
CseI GACGC 1 cut(s) 1067
Csp6I GTAC 1 cut(s) 53
CviAII CATG 4 cut(s) 230, 262, 817, 898
CviQI GTAC 1 cut(s) 53
DdeI CTNAG 6 cut(s) 96, 513, 806, 855, 1091, 1152
DinI GGCGCC 1 cut(s) 303
DpnI GATC 5 cut(s) 63, 151, 438, 839, 1237
DpnII GATC 5 cut(s) 61, 149, 436, 837, 1235
DraI TTTAAA 1 cut(s) 726
EaeI YGGCCR 2 cut(s) 287, 933
EagI CGGCCG 1 cut(s) 287
Eam1104I CTCTTC 2 cut(s) 180, 414
EarI CTCTTC 2 cut(s) 180, 414
EclXI CGGCCG 1 cut(s) 287
Eco130I CCWWGG 1 cut(s) 969
Eco47I GGWCC 1 cut(s) 226
Eco47III AGCGCT 2 cut(s) 522, 831
Eco52I CGGCCG 1 cut(s) 287
Eco57I CTGAAG 3 cut(s) 91, 575, 1094
Eco88I CYCGRG 2 cut(s) 885, 1196
EcoO109I RGGNCCY 1 cut(s) 869
EcoT14I CCWWGG 1 cut(s) 969
EgeI GGCGCC 1 cut(s) 303
EheI GGCGCC 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 969
Esp3I CGTCTC 1 cut(s) 996
FaeI CATG 4 cut(s) 233, 265, 820, 901
FatI CATG 4 cut(s) 229, 261, 816, 897
Fnu4HI GCNGC 5 cut(s) 93, 287, 497, 560, 1134
FokI GGATG 7 cut(s) 22, 230, 379, 860, 926, 972, 986
Fsp4HI GCNGC 5 cut(s) 93, 287, 497, 560, 1134
FspBI CTAG 3 cut(s) 518, 611, 1130
GlaI GCGC 6 cut(s) 201, 303, 495, 522, 563, 831
GluI GCNGC 5 cut(s) 93, 287, 497, 560, 1134
GsuI CTGGAG 1 cut(s) 978
HaeII RGCGCY 5 cut(s) 203, 305, 524, 565, 833
HaeIII GGCC 3 cut(s) 289, 871, 935
HapII CCGG 1 cut(s) 290
HgaI GACGC 1 cut(s) 1067
HhaI GCGC 6 cut(s) 202, 304, 496, 523, 564, 832
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 4 cut(s) 233, 265, 820, 901
Hin6I GCGC 6 cut(s) 200, 302, 494, 521, 562, 830
HinP1I GCGC 6 cut(s) 200, 302, 494, 521, 562, 830
HincII GTYRAC 1 cut(s) 459
HindII GTYRAC 1 cut(s) 459
HinfI GANTC 3 cut(s) 635, 929, 1069
HpaII CCGG 1 cut(s) 290
HphI GGTGA 2 cut(s) 626, 1151
Hpy166II GTNNAC 2 cut(s) 459, 904
Hpy188I TCNGA 6 cut(s) 210, 535, 964, 1054, 1074, 1164
Hpy188III TCNNGA 7 cut(s) 364, 440, 580, 812, 887, 995, 1151
Hpy8I GTNNAC 2 cut(s) 459, 904
HpyAV CCTTC 4 cut(s) 184, 392, 502, 1165
HpyCH4III ACNGT 3 cut(s) 57, 123, 164
HpyCH4IV ACGT 2 cut(s) 347, 796
HpyCH4V TGCA 5 cut(s) 171, 370, 427, 450, 1010
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 125, 322, 518, 559
HpyF3I CTNAG 6 cut(s) 96, 513, 806, 855, 1091, 1152
HpySE526I ACGT 2 cut(s) 347, 796
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 4 cut(s) 233, 265, 820, 901
HspAI GCGC 6 cut(s) 200, 302, 494, 521, 562, 830
KasI GGCGCC 1 cut(s) 301
Kzo9I GATC 5 cut(s) 61, 149, 436, 837, 1235
LmnI GCTCC 1 cut(s) 283
Lsp1109I GCAGC 4 cut(s) 104, 508, 571, 1120
LweI GCATC 5 cut(s) 357, 414, 838, 904, 994
MaeI CTAG 3 cut(s) 518, 611, 1130
MaeII ACGT 2 cut(s) 347, 796
MaeIII GTNAC 1 cut(s) 353
MalI GATC 5 cut(s) 63, 151, 438, 839, 1237
MbiI CCGCTC 1 cut(s) 286
MboI GATC 5 cut(s) 61, 149, 436, 837, 1235
MboII GAAGA 7 cut(s) 142, 197, 223, 431, 836, 868, 1187
MfeI CAATTG 1 cut(s) 754
MflI RGATCY 2 cut(s) 61, 149
MluCI AATT 9 cut(s) 24, 112, 137, 754, 957, 979, 1084, 1142, 1208
Mly113I GGCGCC 1 cut(s) 302
MmeI TCCRAC 3 cut(s) 39, 204, 513
MroXI GAANNNNTTC 2 cut(s) 1059, 1146
MseI TTAA 6 cut(s) 252, 525, 725, 843, 1017, 1119
MslI CAYNNNNRTG 1 cut(s) 668
MspA1I CMGCKG 2 cut(s) 95, 553
MspCI CTTAAG 1 cut(s) 1118
MspI CCGG 1 cut(s) 290
MunI CAATTG 1 cut(s) 754
Mva1269I GAATGC 1 cut(s) 737
MwoI GCNNNNNNNGC 5 cut(s) 108, 125, 322, 518, 559
NarI GGCGCC 1 cut(s) 302
NdeII GATC 5 cut(s) 61, 149, 436, 837, 1235
NheI GCTAGC 1 cut(s) 517
NlaIII CATG 4 cut(s) 233, 265, 820, 901
NlaIV GGNNCC 2 cut(s) 151, 303
NspI RCATGY 1 cut(s) 820
PaeR7I CTCGAG 1 cut(s) 885
PciI ACATGT 1 cut(s) 816
PctI GAATGC 1 cut(s) 737
PdmI GAANNNNTTC 2 cut(s) 1059, 1146
PfeI GAWTC 3 cut(s) 635, 929, 1069
Pfl23II CGTACG 1 cut(s) 52
PkrI GCNGC 5 cut(s) 94, 288, 498, 561, 1135
PluTI GGCGCC 1 cut(s) 305
Ppu21I YACGTR 1 cut(s) 797
PscI ACATGT 1 cut(s) 816
PspLI CGTACG 1 cut(s) 52
PspN4I GGNNCC 2 cut(s) 151, 303
PspPI GGNCC 2 cut(s) 226, 869
PsuI RGATCY 2 cut(s) 61, 149
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 1 cut(s) 54
RsaNI GTAC 1 cut(s) 53
RseI CAYNNNNRTG 1 cut(s) 668
SaqAI TTAA 6 cut(s) 252, 525, 725, 843, 1017, 1119
SatI GCNGC 5 cut(s) 93, 287, 497, 560, 1134
Sau3AI GATC 5 cut(s) 61, 149, 436, 837, 1235
Sau96I GGNCC 2 cut(s) 226, 869
SfaNI GCATC 5 cut(s) 357, 414, 838, 904, 994
SfoI GGCGCC 1 cut(s) 303
Sfr274I CTCGAG 1 cut(s) 885
SinI GGWCC 1 cut(s) 226
SlaI CTCGAG 1 cut(s) 885
SmiMI CAYNNNNRTG 1 cut(s) 668
SmlI CTYRAG 4 cut(s) 641, 885, 1118, 1202
SmoI CTYRAG 4 cut(s) 641, 885, 1118, 1202
Sse9I AATT 9 cut(s) 24, 112, 137, 754, 957, 979, 1084, 1142, 1208
SsiI CCGC 2 cut(s) 286, 551
SspDI GGCGCC 1 cut(s) 301
SspMI CTAG 3 cut(s) 518, 611, 1130
StyI CCWWGG 1 cut(s) 969
TaaI ACNGT 3 cut(s) 57, 123, 164
TaiI ACGT 2 cut(s) 350, 799
TaqI TCGA 2 cut(s) 886, 1238
TasI AATT 9 cut(s) 24, 112, 137, 754, 957, 979, 1084, 1142, 1208
TauI GCSGC 1 cut(s) 289
TfiI GAWTC 3 cut(s) 635, 929, 1069
Tru1I TTAA 6 cut(s) 252, 525, 725, 843, 1017, 1119
Tru9I TTAA 6 cut(s) 252, 525, 725, 843, 1017, 1119
TscAI CASTG 1 cut(s) 607
TseI GCWGC 4 cut(s) 92, 496, 559, 1133
TspDTI ATGAA 5 cut(s) 17, 335, 837, 886, 1052
TspGWI ACGGA 2 cut(s) 162, 978
TspRI CASTG 1 cut(s) 607
Vha464I CTTAAG 1 cut(s) 1118
VpaK11BI GGWCC 1 cut(s) 226
XapI RAATTY 1 cut(s) 24
XceI RCATGY 1 cut(s) 820
XcmI CCANNNNNNNNNTGG 1 cut(s) 943
XhoI CTCGAG 1 cut(s) 885
XmnI GAANNNNTTC 2 cut(s) 1059, 1146
XspI CTAG 3 cut(s) 518, 611, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.