Rroxscaffold_6G00394810

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0006200
Physical Location & Seq
Forward (+)
15676618 .. 15680092
3475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00394810.1

Sequence Viewer

Length: 1338 bp
ATGGAGGGCAGAACGGTCAAGAAATATGAGCTCGAGCGCTGCATCAAGGAGCTCCGCAAGTACCGCCAGTACCAGAATGCTCTCGAGATAATGGAATGGATGGAGTTTCGGAAAATTCATTATGCTTTGCCTGACTATGCCGTACGGTTGGATCTTACGGCGAAAGCCAAAGGGATAGAGGCAGCTGAGAGTTACTTCAGCAATTTAGCACCGTCTGCGAAGAACAAATTGACTTACGGATCCCTTTTGAACTGTTATTGCAAGGAAGTGATGGAAGAGAAGGCATTGGCGCTTTATCAGAAGATGGATGAGTTGGACTTTGCTAACTCTACTCTGGTGTTTAACAATCTCATGTCTTTGTATATGAGAAAGGAGCGGCCGGAGAAAGTGGCACCTTTCGTAGAGCAAATGAAGCAGAGGGAGATATCTTTAGATACGTTTAGTTACAATATCTGGATGCAGAGCTATGCCTCTTTGAATGATATTGAAGGCGTGGAGAGAGTTGTGGAAGAGATGCAAAGCCAAGATCAAGACAAATGCAACTGGTCAACATATAGTAATCTGGCTTCCATCTATGTAAAGGCGCAGCTTTTTGAGAAGGCTGAGCTAGCGCTTAAAATGTCGGAGAAAATGATGATACCGCTGAAGCAGCGCCAGACATACCATTTCTTGATTACCCTCTACGCCAACACTGGTAATCTAGGTGAGGTCAAAAGAATATGGGAATCCCTCAAGTTAGCTTTCCCAGAAACCACCAATATGAGCTATATACTTATGCTTCAAGCTCTATGTAAGCTAAAAGATGTTGAGGGTTTGAAAGAATGCTTTGAGGAGTGGCAGTCCAATTGCTCCAGTTATGATATGAGGTTAGCCAATGTTGTTATACGTGCTTATCTTAGTCAGGACATGTATGAAGAAGCGCTGTTGATCTTTAAGGATGCTACTAAGAGGTCTAAAGGGCCTTTCTTCAAGGCTCGAGAAATGTTCATGGTTTACTTCTTGGATGCCCGTCAGGTTGATTCGGCCATTAGCTATTTGGGAGAGGCGATTTCAGAAACCAAGGATGAGAATTGGCATCCGTCTCCAGAAACCATTGGTGCATTTTTTAAGTATTTTGAGGAAACAAAAGATGTTGATAGCGCTGAAAACTTTTGTAAGATTCTGAAGCGTCTAAATTTTCTCAGTTCCAATGAGTATTCTTTGTTGCTTAAGGTTTATCTAGCTGCTGGTGAATTGCTTCCTGAGATATGTCAGAGATTGAAGGAAGACAACATTCAAATAAGCCCCGAGCTTGAGGAATTGGTGGAGAGGGTTTCCCCAAGTAGATCGACAGCATAG

Protein Analysis

445

Amino Acids

52.03

Weight (kDa)

5.33

Isoelectric Point (pI)

49.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 111 - 157 2.5e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 391
AccBSI CCGCTC 1 cut(s) 376
AciI CCGC 4 cut(s) 55, 64, 376, 641
AclWI GGATC 3 cut(s) 159, 234, 247
AcoI YGGCCR 2 cut(s) 377, 1023
AcsI RAATTY 2 cut(s) 114, 1174
AcuI CTGAAG 3 cut(s) 181, 665, 1184
AfaI GTAC 3 cut(s) 62, 71, 144
AfeI AGCGCT 4 cut(s) 38, 612, 921, 1141
AflII CTTAAG 1 cut(s) 1208
AflIII ACRYGT 1 cut(s) 906
AgsI TTSAA 8 cut(s) 250, 478, 488, 782, 817, 970, 1261, 1277
Alw21I GWGCWC 2 cut(s) 33, 54
Alw26I GTCTC 1 cut(s) 1086
AlwI GGATC 3 cut(s) 159, 234, 247
Ama87I CYCGRG 4 cut(s) 32, 83, 975, 1286
Aor51HI AGCGCT 4 cut(s) 38, 612, 921, 1141
AoxI GGCC 3 cut(s) 377, 959, 1023
ApeKI GCWGC 5 cut(s) 39, 182, 586, 649, 1223
ApoI RAATTY 2 cut(s) 114, 1174
Asp700I GAANNNNTTC 1 cut(s) 1236
AspLEI GCGC 7 cut(s) 39, 292, 586, 613, 654, 922, 1142
AspS9I GGNCC 1 cut(s) 959
AsuHPI GGTGA 2 cut(s) 716, 1241
AsuNHI GCTAGC 1 cut(s) 607
AvaI CYCGRG 4 cut(s) 32, 83, 975, 1286
BamHI GGATCC 1 cut(s) 239
BanI GGYRCC 1 cut(s) 391
BanII GRGCYC 2 cut(s) 33, 54
BarI GAAGNNNNNNTAC 2 cut(s) 550, 582
BbsI GAAGAC 1 cut(s) 1272
Bbv12I GWGCWC 2 cut(s) 33, 54
BbvI GCAGC 5 cut(s) 26, 194, 598, 661, 1210
BccI CCATC 4 cut(s) 94, 265, 298, 578
BceAI ACGGC 2 cut(s) 125, 174
BcoDI GTCTC 1 cut(s) 1086
BfaI CTAG 3 cut(s) 608, 701, 1220
BfoI RGCGCY 6 cut(s) 40, 293, 614, 655, 923, 1143
BfrI CTTAAG 1 cut(s) 1208
BisI GCNGC 6 cut(s) 40, 183, 377, 587, 650, 1224
BlpI GCTNAGC 1 cut(s) 603
BlsI GCNGC 6 cut(s) 41, 184, 378, 588, 651, 1225
BmeT110I CYCGRG 4 cut(s) 32, 83, 975, 1286
BmgT120I GGNCC 1 cut(s) 959
BmiI GGNNCC 2 cut(s) 241, 393
BmsI GCATC 6 cut(s) 51, 447, 504, 928, 994, 1084
BmtI GCTAGC 1 cut(s) 611
BpiI GAAGAC 1 cut(s) 1272
BpmI CTGGAG 2 cut(s) 835, 1068
Bpu1102I GCTNAGC 1 cut(s) 603
BpuEI CTTGAG 2 cut(s) 716, 1313
BsaAI YACGTR 1 cut(s) 887
BsaJI CCNNGG 1 cut(s) 1059
BsaXI ACNNNNNCTCC 2 cut(s) 824, 854
Bse1I ACTGG 4 cut(s) 67, 548, 697, 852
BseDI CCNNGG 1 cut(s) 1059
BseGI GGATG 7 cut(s) 105, 313, 462, 943, 1009, 1069, 1075
BseMII CTCAG 4 cut(s) 177, 594, 1195, 1233
BseNI ACTGG 4 cut(s) 67, 548, 697, 852
BseRI GAGGAG 1 cut(s) 845
BseX3I CGGCCG 1 cut(s) 377
BseXI GCAGC 5 cut(s) 26, 194, 598, 661, 1210
Bsh1285I CGRYCG 1 cut(s) 380
BshFI GGCC 3 cut(s) 379, 961, 1025
BshNI GGYRCC 1 cut(s) 391
BsiEI CGRYCG 1 cut(s) 380
BsiHKAI GWGCWC 2 cut(s) 33, 54
BsiHKCI CYCGRG 4 cut(s) 32, 83, 975, 1286
BsiSI CCGG 1 cut(s) 380
BsiWI CGTACG 1 cut(s) 142
BsmAI GTCTC 1 cut(s) 1086
BsmBI CGTCTC 1 cut(s) 1086
BsmI GAATGC 2 cut(s) 82, 827
BsnI GGCC 3 cut(s) 379, 961, 1025
BsoBI CYCGRG 4 cut(s) 32, 83, 975, 1286
Bsp1286I GDGCHC 2 cut(s) 33, 54
Bsp143I GATC 5 cut(s) 151, 239, 526, 927, 1325
Bsp1720I GCTNAGC 1 cut(s) 603
BspACI CCGC 4 cut(s) 55, 64, 376, 641
BspANI GGCC 3 cut(s) 379, 961, 1025
BspCNI CTCAG 4 cut(s) 178, 595, 1194, 1234
BspLI GGNNCC 2 cut(s) 241, 393
BspOI GCTAGC 1 cut(s) 611
BspPI GGATC 3 cut(s) 159, 234, 247
BspT107I GGYRCC 1 cut(s) 391
BspTI CTTAAG 1 cut(s) 1208
BsrBI CCGCTC 1 cut(s) 376
BsrI ACTGG 4 cut(s) 67, 548, 697, 852
BssECI CCNNGG 1 cut(s) 1059
BssMI GATC 5 cut(s) 151, 239, 526, 927, 1325
BssT1I CCWWGG 1 cut(s) 1059
Bst4CI ACNGT 4 cut(s) 16, 147, 213, 254
Bst6I CTCTTC 2 cut(s) 270, 504
BstAFI CTTAAG 1 cut(s) 1208
BstAPI GCANNNNNTGC 1 cut(s) 215
BstBAI YACGTR 1 cut(s) 887
BstC8I GCNNGC 1 cut(s) 609
BstDEI CTNAG 6 cut(s) 186, 603, 896, 945, 1181, 1242
BstF5I GGATG 7 cut(s) 105, 313, 462, 943, 1009, 1069, 1075
BstH2I RGCGCY 6 cut(s) 40, 293, 614, 655, 923, 1143
BstHHI GCGC 7 cut(s) 39, 292, 586, 613, 654, 922, 1142
BstKTI GATC 5 cut(s) 154, 242, 529, 930, 1328
BstMAI GTCTC 1 cut(s) 1086
BstMBI GATC 5 cut(s) 151, 239, 526, 927, 1325
BstMCI CGRYCG 1 cut(s) 380
BstMWI GCNNNNNNNGC 5 cut(s) 63, 215, 412, 608, 649
BstNSI RCATGY 1 cut(s) 910
BstV1I GCAGC 5 cut(s) 26, 194, 598, 661, 1210
BstV2I GAAGAC 1 cut(s) 1272
BstX2I RGATCY 2 cut(s) 151, 239
BstYI RGATCY 2 cut(s) 151, 239
BstZI CGGCCG 1 cut(s) 377
BsuRI GGCC 3 cut(s) 379, 961, 1025
BtsCI GGATG 7 cut(s) 105, 313, 462, 943, 1009, 1069, 1075
BtsIMutI CAGTG 1 cut(s) 690
Cac8I GCNNGC 1 cut(s) 609
CfoI GCGC 7 cut(s) 39, 292, 586, 613, 654, 922, 1142
Cfr13I GGNCC 1 cut(s) 959
CseI GACGC 1 cut(s) 1157
Csp6I GTAC 3 cut(s) 61, 70, 143
CviAII CATG 3 cut(s) 352, 907, 988
CviQI GTAC 3 cut(s) 61, 70, 143
DdeI CTNAG 6 cut(s) 186, 603, 896, 945, 1181, 1242
DpnI GATC 5 cut(s) 153, 241, 528, 929, 1327
DpnII GATC 5 cut(s) 151, 239, 526, 927, 1325
EaeI YGGCCR 2 cut(s) 377, 1023
EagI CGGCCG 1 cut(s) 377
Eam1104I CTCTTC 2 cut(s) 270, 504
EarI CTCTTC 2 cut(s) 270, 504
Ecl136II GAGCTC 2 cut(s) 31, 52
EclXI CGGCCG 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 1059
Eco24I GRGCYC 2 cut(s) 33, 54
Eco32I GATATC 1 cut(s) 426
Eco47III AGCGCT 4 cut(s) 38, 612, 921, 1141
Eco52I CGGCCG 1 cut(s) 377
Eco53kI GAGCTC 2 cut(s) 31, 52
Eco57I CTGAAG 3 cut(s) 181, 665, 1184
Eco88I CYCGRG 4 cut(s) 32, 83, 975, 1286
EcoICRI GAGCTC 2 cut(s) 31, 52
EcoO109I RGGNCCY 1 cut(s) 959
EcoRV GATATC 1 cut(s) 426
EcoT14I CCWWGG 1 cut(s) 1059
EcoT38I GRGCYC 2 cut(s) 33, 54
ErhI CCWWGG 1 cut(s) 1059
Esp3I CGTCTC 1 cut(s) 1086
FaeI CATG 3 cut(s) 355, 910, 991
FatI CATG 3 cut(s) 351, 906, 987
Fnu4HI GCNGC 6 cut(s) 40, 183, 377, 587, 650, 1224
FokI GGATG 7 cut(s) 112, 320, 469, 950, 1016, 1062, 1076
FriOI GRGCYC 2 cut(s) 33, 54
Fsp4HI GCNGC 6 cut(s) 40, 183, 377, 587, 650, 1224
FspBI CTAG 3 cut(s) 608, 701, 1220
GlaI GCGC 7 cut(s) 38, 291, 585, 612, 653, 921, 1141
GluI GCNGC 6 cut(s) 40, 183, 377, 587, 650, 1224
GsuI CTGGAG 2 cut(s) 835, 1068
HaeII RGCGCY 6 cut(s) 40, 293, 614, 655, 923, 1143
HaeIII GGCC 3 cut(s) 379, 961, 1025
HapII CCGG 1 cut(s) 380
HgaI GACGC 1 cut(s) 1157
HhaI GCGC 7 cut(s) 39, 292, 586, 613, 654, 922, 1142
Hin1II CATG 3 cut(s) 355, 910, 991
Hin6I GCGC 7 cut(s) 37, 290, 584, 611, 652, 920, 1140
HinP1I GCGC 7 cut(s) 37, 290, 584, 611, 652, 920, 1140
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HinfI GANTC 3 cut(s) 725, 1019, 1159
HpaII CCGG 1 cut(s) 380
HphI GGTGA 2 cut(s) 716, 1241
Hpy166II GTNNAC 2 cut(s) 549, 994
Hpy188I TCNGA 6 cut(s) 111, 300, 625, 1054, 1164, 1254
Hpy8I GTNNAC 2 cut(s) 549, 994
HpyAV CCTTC 4 cut(s) 274, 482, 592, 1255
HpyCH4III ACNGT 4 cut(s) 16, 147, 213, 254
HpyCH4IV ACGT 2 cut(s) 437, 886
HpyCH4V TGCA 6 cut(s) 42, 261, 460, 517, 540, 1100
HpyF10VI GCNNNNNNNGC 5 cut(s) 63, 215, 412, 608, 649
HpyF3I CTNAG 6 cut(s) 186, 603, 896, 945, 1181, 1242
HpySE526I ACGT 2 cut(s) 437, 886
Hsp92II CATG 3 cut(s) 355, 910, 991
HspAI GCGC 7 cut(s) 37, 290, 584, 611, 652, 920, 1140
Kzo9I GATC 5 cut(s) 151, 239, 526, 927, 1325
LmnI GCTCC 4 cut(s) 49, 57, 373, 854
Lsp1109I GCAGC 5 cut(s) 26, 194, 598, 661, 1210
LweI GCATC 6 cut(s) 51, 447, 504, 928, 994, 1084
MaeI CTAG 3 cut(s) 608, 701, 1220
MaeII ACGT 2 cut(s) 437, 886
MaeIII GTNAC 2 cut(s) 191, 443
MalI GATC 5 cut(s) 153, 241, 528, 929, 1327
MbiI CCGCTC 1 cut(s) 376
MboI GATC 5 cut(s) 151, 239, 526, 927, 1325
MboII GAAGA 7 cut(s) 232, 287, 313, 521, 926, 958, 1277
MfeI CAATTG 1 cut(s) 844
MflI RGATCY 2 cut(s) 151, 239
MhlI GDGCHC 2 cut(s) 33, 54
MluCI AATT 8 cut(s) 114, 202, 227, 844, 1069, 1174, 1232, 1298
MmeI TCCRAC 3 cut(s) 129, 294, 603
MroXI GAANNNNTTC 1 cut(s) 1236
MseI TTAA 5 cut(s) 342, 615, 933, 1107, 1209
MslI CAYNNNNRTG 1 cut(s) 758
MspA1I CMGCKG 2 cut(s) 185, 643
MspCI CTTAAG 1 cut(s) 1208
MspI CCGG 1 cut(s) 380
MunI CAATTG 1 cut(s) 844
Mva1269I GAATGC 2 cut(s) 82, 827
MwoI GCNNNNNNNGC 5 cut(s) 63, 215, 412, 608, 649
NdeII GATC 5 cut(s) 151, 239, 526, 927, 1325
NheI GCTAGC 1 cut(s) 607
NlaIII CATG 3 cut(s) 355, 910, 991
NlaIV GGNNCC 2 cut(s) 241, 393
NspI RCATGY 1 cut(s) 910
PaeR7I CTCGAG 3 cut(s) 32, 83, 975
PciI ACATGT 1 cut(s) 906
PctI GAATGC 2 cut(s) 82, 827
PdmI GAANNNNTTC 1 cut(s) 1236
PfeI GAWTC 3 cut(s) 725, 1019, 1159
Pfl23II CGTACG 1 cut(s) 142
PkrI GCNGC 6 cut(s) 41, 184, 378, 588, 651, 1225
Ppu21I YACGTR 1 cut(s) 887
PscI ACATGT 1 cut(s) 906
Psp124BI GAGCTC 2 cut(s) 33, 54
PspLI CGTACG 1 cut(s) 142
PspN4I GGNNCC 2 cut(s) 241, 393
PspPI GGNCC 1 cut(s) 959
PspXI VCTCGAGB 1 cut(s) 32
PsuI RGATCY 2 cut(s) 151, 239
PvuII CAGCTG 1 cut(s) 185
RsaI GTAC 3 cut(s) 62, 71, 144
RsaNI GTAC 3 cut(s) 61, 70, 143
RseI CAYNNNNRTG 1 cut(s) 758
SacI GAGCTC 2 cut(s) 33, 54
SaqAI TTAA 5 cut(s) 342, 615, 933, 1107, 1209
SatI GCNGC 6 cut(s) 40, 183, 377, 587, 650, 1224
Sau3AI GATC 5 cut(s) 151, 239, 526, 927, 1325
Sau96I GGNCC 1 cut(s) 959
SduI GDGCHC 2 cut(s) 33, 54
SfaNI GCATC 6 cut(s) 51, 447, 504, 928, 994, 1084
Sfr274I CTCGAG 3 cut(s) 32, 83, 975
SlaI CTCGAG 3 cut(s) 32, 83, 975
SmiMI CAYNNNNRTG 1 cut(s) 758
SmlI CTYRAG 6 cut(s) 32, 83, 731, 975, 1208, 1292
SmoI CTYRAG 6 cut(s) 32, 83, 731, 975, 1208, 1292
Sse9I AATT 8 cut(s) 114, 202, 227, 844, 1069, 1174, 1232, 1298
SsiI CCGC 4 cut(s) 55, 64, 376, 641
SspMI CTAG 3 cut(s) 608, 701, 1220
SstI GAGCTC 2 cut(s) 33, 54
StyI CCWWGG 1 cut(s) 1059
TaaI ACNGT 4 cut(s) 16, 147, 213, 254
TaiI ACGT 2 cut(s) 440, 889
TaqI TCGA 4 cut(s) 33, 84, 976, 1328
TasI AATT 8 cut(s) 114, 202, 227, 844, 1069, 1174, 1232, 1298
TauI GCSGC 1 cut(s) 379
TfiI GAWTC 3 cut(s) 725, 1019, 1159
Tru1I TTAA 5 cut(s) 342, 615, 933, 1107, 1209
Tru9I TTAA 5 cut(s) 342, 615, 933, 1107, 1209
TscAI CASTG 1 cut(s) 697
TseI GCWGC 5 cut(s) 39, 182, 586, 649, 1223
TspDTI ATGAA 4 cut(s) 107, 425, 927, 976
TspGWI ACGGA 2 cut(s) 252, 1068
TspRI CASTG 1 cut(s) 697
Vha464I CTTAAG 1 cut(s) 1208
XapI RAATTY 2 cut(s) 114, 1174
XceI RCATGY 1 cut(s) 910
XcmI CCANNNNNNNNNTGG 1 cut(s) 1033
XhoI CTCGAG 3 cut(s) 32, 83, 975
XmnI GAANNNNTTC 1 cut(s) 1236
XspI CTAG 3 cut(s) 608, 701, 1220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.