Rh3AG281800

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0006200
Physical Location & Seq
Reverse (-)
32874705 .. 32878509
3805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG281800.1

Sequence Viewer

Length: 1584 bp
ATGGCGGGTATCCTCTTAAACCCTAACAGAAACACAAAAGTTACTTTAAACCCTTGTTTGAGAGAGAGAGAGAGAATGAATCGTAGCCGTTGGATGATGTCGTCGGGAGCGGGGGAGCTGGTGAGGAAGCTCTGTACGGCGGTGGCGGCGGGGGGTCGGCGTGACAATCCGGATAGGCTGTACCGGAGGTTATCGGCTTTGGGAGGAAGCGGGAGGAGCGCGGCCACGGCCTTGAATGATTACATCATGGAGGGCAGAACGGTCAAGAAATATGAGCTCGAGCGCTGCATCAAGGAGCTCCGCAAGTACCGCCAGTACCAGAATGCTCTCGAGATAATGGAATGGATGGAGTTTAGGAAAATTCATTATGCTTTGCCTGACTATGCCGTACGGTTGGATCTTACGGCGAAAGCCAAAGGGATAGAGGCAGCTGAGAGTTACTTCAGCAATTTAGCACCGTCTGCGAAGAACAAATTGACTTACGGATCCCTTTTGAACTGTTATTGCAAGGAAGTGATGGAAGAGAAGGCATTGGCGCTTTATCAGAAGATGGATGAGTTGGACTGTGCCAACTCTACTCTGGTTTTTAACAATCTCATGTCTTTGTATATGAGAAAGGAGCGGCCGGAGAAAGTGGCGCCTTTCGTAGAGCAAATGAAGCAGAGGGAGATATCTTTAGATACGTTTAGTTACAATATCTGGATGCAGAGCTATGCCTCTTTGAATGATATTGAAGGCGTGGAGAGAGTTGTGGAAGAGATGCAAAGCCAAGATCAAGACAAATGCAACTGGTCAACATATAGTAATCTGGCTTCCATCTATGTAAAGGCGCAGCTTTTTGAGAAGGCTGAGCTAGCGCTTAAAATGTCGGAGAAAATGATGATACCGCTGAAGCAGCGCCAGACGTACCATTTCTTGATTACCCTCTATGCCAACACTGGTAATCTAGGTGAGGTCAAAAGAATATGGGAATCCCTCAAGTTAGCTTTCCCAGAAACCACCAATATGAGCTATATACTTGTGCTTCAAGCTCTATGTAAGCTAAAAGATGTTGAGGGTTTAAAAGAATGCTTTGAGGAGTGGCAGTCCAATTGCTCCAGTTATGATATGAGGTTAGCAAATGTAGTTATACGTGCTTATCTTAGTCAGGACATGCATGAAGAAGCGCTGTTGATCTTTAAGGATGCTACTAAGAGGTCTAAAGGGCCTTTCTTCAAGGCTCGAGAAATGTTCATGGTTTACTTCTTGGATGCCCGTCAGGTTGATTCAGCCATTAGCTATTTGGGAGAGGCGATTTCAGAAACCAAGGATGAGAATTGGCATCCGTCTCCAGAAATCATTGGTGCATTTTTTAAGTATTTTGAGGAAACAAAAGATGTTGATAGCTCTGAAAACTTTCATAAGATTCTGAAGCGTCTAAATTGTCTCAGTTCCAATGAGTATTCTTTGTTGCTTAAGGTTTATCTAGCAGCTGGTGAATTACTTCCTGAGATATGTCAGAGATTGAAGGAAGACAACATTCAAATAAGCCCCGAGCTTGAGGAATTGGTGGAGAGGGTTTCCCCAACTACATCGACAGCATAG

Protein Analysis

527

Amino Acids

60.94

Weight (kDa)

6.61

Isoelectric Point (pI)

52.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 193 - 239 3.1e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 637
AccBSI CCGCTC 2 cut(s) 110, 622
AccII CGCG 1 cut(s) 221
AccIII TCCGGA 1 cut(s) 169
AclWI GGATC 3 cut(s) 405, 480, 493
AcoI YGGCCR 2 cut(s) 222, 623
AcsI RAATTY 1 cut(s) 360
AcuI CTGAAG 3 cut(s) 427, 911, 1430
AcyI GRCGYC 1 cut(s) 638
AfaI GTAC 6 cut(s) 136, 182, 308, 317, 390, 908
AfeI AGCGCT 3 cut(s) 284, 858, 1167
AflII CTTAAG 1 cut(s) 1454
AgsI TTSAA 8 cut(s) 235, 496, 724, 734, 1028, 1216, 1507, 1523
Alw21I GWGCWC 2 cut(s) 279, 300
Alw26I GTCTC 2 cut(s) 1332, 1430
AlwI GGATC 3 cut(s) 405, 480, 493
Ama87I CYCGRG 4 cut(s) 278, 329, 1221, 1532
Aor13HI TCCGGA 1 cut(s) 169
Aor51HI AGCGCT 3 cut(s) 284, 858, 1167
AoxI GGCC 4 cut(s) 222, 228, 623, 1205
ApeKI GCWGC 5 cut(s) 285, 428, 832, 895, 1469
ApoI RAATTY 1 cut(s) 360
Asp700I GAANNNNTTC 2 cut(s) 1395, 1482
AspLEI GCGC 8 cut(s) 221, 285, 538, 640, 832, 859, 900, 1168
AspS9I GGNCC 1 cut(s) 1205
AsuHPI GGTGA 3 cut(s) 133, 962, 1487
AsuNHI GCTAGC 1 cut(s) 853
AvaI CYCGRG 4 cut(s) 278, 329, 1221, 1532
BamHI GGATCC 1 cut(s) 485
BanI GGYRCC 1 cut(s) 637
BanII GRGCYC 2 cut(s) 279, 300
BarI GAAGNNNNNNTAC 2 cut(s) 796, 828
BbsI GAAGAC 1 cut(s) 1518
Bbv12I GWGCWC 2 cut(s) 279, 300
BbvI GCAGC 5 cut(s) 272, 440, 844, 907, 1481
BccI CCATC 4 cut(s) 340, 511, 544, 824
BceAI ACGGC 5 cut(s) 72, 153, 243, 371, 420
BciVI GTATCC 1 cut(s) 20
BcoDI GTCTC 2 cut(s) 1332, 1430
BfaI CTAG 3 cut(s) 854, 947, 1466
BfoI RGCGCY 6 cut(s) 286, 539, 641, 860, 901, 1169
BfrI CTTAAG 1 cut(s) 1454
BfuI GTATCC 1 cut(s) 20
BisI GCNGC 8 cut(s) 147, 222, 286, 429, 623, 833, 896, 1470
BlpI GCTNAGC 1 cut(s) 849
BlsI GCNGC 8 cut(s) 148, 223, 287, 430, 624, 834, 897, 1471
BmeT110I CYCGRG 4 cut(s) 278, 329, 1221, 1532
BmgT120I GGNCC 1 cut(s) 1205
BmiI GGNNCC 2 cut(s) 487, 639
BmsI GCATC 6 cut(s) 297, 693, 750, 1174, 1240, 1330
BmtI GCTAGC 1 cut(s) 857
BpiI GAAGAC 1 cut(s) 1518
BpmI CTGGAG 2 cut(s) 1081, 1314
Bpu1102I GCTNAGC 1 cut(s) 849
BpuEI CTTGAG 2 cut(s) 962, 1559
BsaAI YACGTR 1 cut(s) 1133
BsaHI GRCGYC 1 cut(s) 638
BsaJI CCNNGG 2 cut(s) 225, 1305
BsaWI WCCGGW 2 cut(s) 169, 183
BsaXI ACNNNNNCTCC 2 cut(s) 1070, 1100
Bse1I ACTGG 4 cut(s) 313, 794, 943, 1098
BseAI TCCGGA 1 cut(s) 169
BseDI CCNNGG 2 cut(s) 225, 1305
BseGI GGATG 8 cut(s) 99, 351, 559, 708, 1189, 1255, 1315, 1321
BseMII CTCAG 4 cut(s) 423, 840, 1441, 1479
BseNI ACTGG 4 cut(s) 313, 794, 943, 1098
BseRI GAGGAG 2 cut(s) 229, 1091
BseX3I CGGCCG 1 cut(s) 623
BseXI GCAGC 5 cut(s) 272, 440, 844, 907, 1481
Bsh1236I CGCG 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 626
BshFI GGCC 4 cut(s) 224, 230, 625, 1207
BshNI GGYRCC 1 cut(s) 637
BsiEI CGRYCG 1 cut(s) 626
BsiHKAI GWGCWC 2 cut(s) 279, 300
BsiHKCI CYCGRG 4 cut(s) 278, 329, 1221, 1532
BsiSI CCGG 3 cut(s) 170, 184, 626
BsiWI CGTACG 1 cut(s) 388
BsmAI GTCTC 2 cut(s) 1332, 1430
BsmBI CGTCTC 1 cut(s) 1332
BsmI GAATGC 2 cut(s) 328, 1073
BsnI GGCC 4 cut(s) 224, 230, 625, 1207
BsoBI CYCGRG 4 cut(s) 278, 329, 1221, 1532
Bsp1286I GDGCHC 2 cut(s) 279, 300
Bsp13I TCCGGA 1 cut(s) 169
Bsp143I GATC 4 cut(s) 397, 485, 772, 1173
Bsp1720I GCTNAGC 1 cut(s) 849
BspANI GGCC 4 cut(s) 224, 230, 625, 1207
BspCNI CTCAG 4 cut(s) 424, 841, 1440, 1480
BspEI TCCGGA 1 cut(s) 169
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 2 cut(s) 487, 639
BspOI GCTAGC 1 cut(s) 857
BspPI GGATC 3 cut(s) 405, 480, 493
BspT107I GGYRCC 1 cut(s) 637
BspTI CTTAAG 1 cut(s) 1454
BsrBI CCGCTC 2 cut(s) 110, 622
BsrI ACTGG 4 cut(s) 313, 794, 943, 1098
BssECI CCNNGG 2 cut(s) 225, 1305
BssMI GATC 4 cut(s) 397, 485, 772, 1173
BssNI GRCGYC 1 cut(s) 638
BssT1I CCWWGG 1 cut(s) 1305
Bst4CI ACNGT 5 cut(s) 262, 393, 459, 500, 566
Bst6I CTCTTC 2 cut(s) 516, 750
BstACI GRCGYC 1 cut(s) 638
BstAFI CTTAAG 1 cut(s) 1454
BstAPI GCANNNNNTGC 1 cut(s) 461
BstBAI YACGTR 1 cut(s) 1133
BstC8I GCNNGC 1 cut(s) 855
BstDEI CTNAG 6 cut(s) 432, 849, 1142, 1191, 1427, 1488
BstDSI CCRYGG 1 cut(s) 225
BstF5I GGATG 8 cut(s) 99, 351, 559, 708, 1189, 1255, 1315, 1321
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 6 cut(s) 286, 539, 641, 860, 901, 1169
BstHHI GCGC 8 cut(s) 221, 285, 538, 640, 832, 859, 900, 1168
BstKTI GATC 4 cut(s) 400, 488, 775, 1176
BstMAI GTCTC 2 cut(s) 1332, 1430
BstMBI GATC 4 cut(s) 397, 485, 772, 1173
BstMCI CGRYCG 1 cut(s) 626
BstMWI GCNNNNNNNGC 8 cut(s) 146, 216, 227, 309, 461, 658, 854, 895
BstNSI RCATGY 1 cut(s) 1156
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 5 cut(s) 272, 440, 844, 907, 1481
BstV2I GAAGAC 1 cut(s) 1518
BstX2I RGATCY 2 cut(s) 397, 485
BstYI RGATCY 2 cut(s) 397, 485
BstZI CGGCCG 1 cut(s) 623
BsuI GTATCC 1 cut(s) 20
BsuRI GGCC 4 cut(s) 224, 230, 625, 1207
BtgI CCRYGG 1 cut(s) 225
BtsCI GGATG 8 cut(s) 99, 351, 559, 708, 1189, 1255, 1315, 1321
BtsIMutI CAGTG 1 cut(s) 936
Cac8I GCNNGC 1 cut(s) 855
CfoI GCGC 8 cut(s) 221, 285, 538, 640, 832, 859, 900, 1168
Cfr13I GGNCC 1 cut(s) 1205
CseI GACGC 1 cut(s) 1403
Csp6I GTAC 6 cut(s) 135, 181, 307, 316, 389, 907
CviAII CATG 5 cut(s) 247, 598, 1153, 1157, 1234
CviQI GTAC 6 cut(s) 135, 181, 307, 316, 389, 907
DdeI CTNAG 6 cut(s) 432, 849, 1142, 1191, 1427, 1488
DinI GGCGCC 1 cut(s) 639
DpnI GATC 4 cut(s) 399, 487, 774, 1175
DpnII GATC 4 cut(s) 397, 485, 772, 1173
DraI TTTAAA 2 cut(s) 48, 1062
EaeI YGGCCR 2 cut(s) 222, 623
EagI CGGCCG 1 cut(s) 623
Eam1104I CTCTTC 2 cut(s) 516, 750
EarI CTCTTC 2 cut(s) 516, 750
Ecl136II GAGCTC 2 cut(s) 277, 298
EclXI CGGCCG 1 cut(s) 623
Eco130I CCWWGG 1 cut(s) 1305
Eco24I GRGCYC 2 cut(s) 279, 300
Eco32I GATATC 1 cut(s) 672
Eco47III AGCGCT 3 cut(s) 284, 858, 1167
Eco52I CGGCCG 1 cut(s) 623
Eco53kI GAGCTC 2 cut(s) 277, 298
Eco57I CTGAAG 3 cut(s) 427, 911, 1430
Eco88I CYCGRG 4 cut(s) 278, 329, 1221, 1532
EcoICRI GAGCTC 2 cut(s) 277, 298
EcoO109I RGGNCCY 1 cut(s) 1205
EcoRV GATATC 1 cut(s) 672
EcoT14I CCWWGG 1 cut(s) 1305
EcoT22I ATGCAT 1 cut(s) 1158
EcoT38I GRGCYC 2 cut(s) 279, 300
EgeI GGCGCC 1 cut(s) 639
EheI GGCGCC 1 cut(s) 639
ErhI CCWWGG 1 cut(s) 1305
Esp3I CGTCTC 1 cut(s) 1332
FaeI CATG 5 cut(s) 250, 601, 1156, 1160, 1237
FatI CATG 5 cut(s) 246, 597, 1152, 1156, 1233
FauI CCCGC 3 cut(s) 103, 142, 203
Fnu4HI GCNGC 8 cut(s) 147, 222, 286, 429, 623, 833, 896, 1470
FokI GGATG 8 cut(s) 106, 358, 566, 715, 1196, 1262, 1308, 1322
FriOI GRGCYC 2 cut(s) 279, 300
Fsp4HI GCNGC 8 cut(s) 147, 222, 286, 429, 623, 833, 896, 1470
FspBI CTAG 3 cut(s) 854, 947, 1466
GlaI GCGC 8 cut(s) 220, 284, 537, 639, 831, 858, 899, 1167
GluI GCNGC 8 cut(s) 147, 222, 286, 429, 623, 833, 896, 1470
GsuI CTGGAG 2 cut(s) 1081, 1314
HaeII RGCGCY 6 cut(s) 286, 539, 641, 860, 901, 1169
HaeIII GGCC 4 cut(s) 224, 230, 625, 1207
HapII CCGG 3 cut(s) 170, 184, 626
HgaI GACGC 1 cut(s) 1403
HhaI GCGC 8 cut(s) 221, 285, 538, 640, 832, 859, 900, 1168
Hin1I GRCGYC 1 cut(s) 638
Hin1II CATG 5 cut(s) 250, 601, 1156, 1160, 1237
Hin6I GCGC 8 cut(s) 219, 283, 536, 638, 830, 857, 898, 1166
HinP1I GCGC 8 cut(s) 219, 283, 536, 638, 830, 857, 898, 1166
HincII GTYRAC 1 cut(s) 795
HindII GTYRAC 1 cut(s) 795
HinfI GANTC 4 cut(s) 79, 971, 1265, 1405
HpaII CCGG 3 cut(s) 170, 184, 626
HphI GGTGA 3 cut(s) 133, 962, 1487
Hpy166II GTNNAC 2 cut(s) 795, 1240
Hpy188I TCNGA 6 cut(s) 546, 871, 1300, 1390, 1410, 1500
Hpy8I GTNNAC 2 cut(s) 795, 1240
Hpy99I CGWCG 1 cut(s) 106
HpyAV CCTTC 4 cut(s) 520, 728, 838, 1501
HpyCH4III ACNGT 5 cut(s) 262, 393, 459, 500, 566
HpyCH4IV ACGT 3 cut(s) 683, 905, 1132
HpyCH4V TGCA 7 cut(s) 288, 507, 706, 763, 786, 1156, 1346
HpyF10VI GCNNNNNNNGC 8 cut(s) 146, 216, 227, 309, 461, 658, 854, 895
HpyF3I CTNAG 6 cut(s) 432, 849, 1142, 1191, 1427, 1488
HpySE526I ACGT 3 cut(s) 683, 905, 1132
Hsp92I GRCGYC 1 cut(s) 638
Hsp92II CATG 5 cut(s) 250, 601, 1156, 1160, 1237
HspAI GCGC 8 cut(s) 219, 283, 536, 638, 830, 857, 898, 1166
KasI GGCGCC 1 cut(s) 637
Kpn2I TCCGGA 1 cut(s) 169
Kzo9I GATC 4 cut(s) 397, 485, 772, 1173
LmnI GCTCC 7 cut(s) 107, 115, 216, 295, 303, 619, 1100
Lsp1109I GCAGC 5 cut(s) 272, 440, 844, 907, 1481
LweI GCATC 6 cut(s) 297, 693, 750, 1174, 1240, 1330
MaeI CTAG 3 cut(s) 854, 947, 1466
MaeII ACGT 3 cut(s) 683, 905, 1132
MaeIII GTNAC 4 cut(s) 40, 161, 437, 689
MalI GATC 4 cut(s) 399, 487, 774, 1175
MbiI CCGCTC 2 cut(s) 110, 622
MboI GATC 4 cut(s) 397, 485, 772, 1173
MboII GAAGA 7 cut(s) 478, 533, 559, 767, 1172, 1204, 1523
MfeI CAATTG 1 cut(s) 1090
MflI RGATCY 2 cut(s) 397, 485
MhlI GDGCHC 2 cut(s) 279, 300
MluCI AATT 8 cut(s) 360, 448, 473, 1090, 1315, 1420, 1478, 1544
Mly113I GGCGCC 1 cut(s) 638
MmeI TCCRAC 4 cut(s) 71, 375, 540, 849
Mph1103I ATGCAT 1 cut(s) 1158
MroI TCCGGA 1 cut(s) 169
MroXI GAANNNNTTC 2 cut(s) 1395, 1482
MseI TTAA 8 cut(s) 17, 47, 588, 861, 1061, 1179, 1353, 1455
MslI CAYNNNNRTG 1 cut(s) 1004
MspA1I CMGCKG 3 cut(s) 431, 889, 1472
MspCI CTTAAG 1 cut(s) 1454
MspI CCGG 3 cut(s) 170, 184, 626
MunI CAATTG 1 cut(s) 1090
Mva1269I GAATGC 2 cut(s) 328, 1073
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 8 cut(s) 146, 216, 227, 309, 461, 658, 854, 895
NarI GGCGCC 1 cut(s) 638
NdeII GATC 4 cut(s) 397, 485, 772, 1173
NheI GCTAGC 1 cut(s) 853
NlaIII CATG 5 cut(s) 250, 601, 1156, 1160, 1237
NlaIV GGNNCC 2 cut(s) 487, 639
NmuCI GTSAC 1 cut(s) 161
NsiI ATGCAT 1 cut(s) 1158
NspI RCATGY 1 cut(s) 1156
PaeR7I CTCGAG 3 cut(s) 278, 329, 1221
PctI GAATGC 2 cut(s) 328, 1073
PdmI GAANNNNTTC 2 cut(s) 1395, 1482
PfeI GAWTC 4 cut(s) 79, 971, 1265, 1405
Pfl23II CGTACG 1 cut(s) 388
PkrI GCNGC 8 cut(s) 148, 223, 287, 430, 624, 834, 897, 1471
PluTI GGCGCC 1 cut(s) 641
Ppu21I YACGTR 1 cut(s) 1133
Psp124BI GAGCTC 2 cut(s) 279, 300
PspLI CGTACG 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 487, 639
PspPI GGNCC 1 cut(s) 1205
PspXI VCTCGAGB 1 cut(s) 278
PsuI RGATCY 2 cut(s) 397, 485
PvuII CAGCTG 2 cut(s) 431, 1472
RsaI GTAC 6 cut(s) 136, 182, 308, 317, 390, 908
RsaNI GTAC 6 cut(s) 135, 181, 307, 316, 389, 907
RseI CAYNNNNRTG 1 cut(s) 1004
SacI GAGCTC 2 cut(s) 279, 300
SaqAI TTAA 8 cut(s) 17, 47, 588, 861, 1061, 1179, 1353, 1455
SatI GCNGC 8 cut(s) 147, 222, 286, 429, 623, 833, 896, 1470
Sau3AI GATC 4 cut(s) 397, 485, 772, 1173
Sau96I GGNCC 1 cut(s) 1205
SduI GDGCHC 2 cut(s) 279, 300
SfaNI GCATC 6 cut(s) 297, 693, 750, 1174, 1240, 1330
SfoI GGCGCC 1 cut(s) 639
Sfr274I CTCGAG 3 cut(s) 278, 329, 1221
SlaI CTCGAG 3 cut(s) 278, 329, 1221
SmiMI CAYNNNNRTG 1 cut(s) 1004
SmlI CTYRAG 6 cut(s) 278, 329, 977, 1221, 1454, 1538
SmoI CTYRAG 6 cut(s) 278, 329, 977, 1221, 1454, 1538
Sse9I AATT 8 cut(s) 360, 448, 473, 1090, 1315, 1420, 1478, 1544
SspDI GGCGCC 1 cut(s) 637
SspMI CTAG 3 cut(s) 854, 947, 1466
SstI GAGCTC 2 cut(s) 279, 300
StyI CCWWGG 1 cut(s) 1305
TaaI ACNGT 5 cut(s) 262, 393, 459, 500, 566
TaiI ACGT 3 cut(s) 686, 908, 1135
TaqI TCGA 4 cut(s) 279, 330, 1222, 1574
TasI AATT 8 cut(s) 360, 448, 473, 1090, 1315, 1420, 1478, 1544
TauI GCSGC 3 cut(s) 149, 224, 625
TfiI GAWTC 4 cut(s) 79, 971, 1265, 1405
Tru1I TTAA 8 cut(s) 17, 47, 588, 861, 1061, 1179, 1353, 1455
Tru9I TTAA 8 cut(s) 17, 47, 588, 861, 1061, 1179, 1353, 1455
TscAI CASTG 1 cut(s) 943
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 5 cut(s) 285, 428, 832, 895, 1469
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 6 cut(s) 92, 353, 671, 1173, 1222, 1388
TspGWI ACGGA 2 cut(s) 498, 1314
TspRI CASTG 1 cut(s) 943
Vha464I CTTAAG 1 cut(s) 1454
XapI RAATTY 1 cut(s) 360
XceI RCATGY 1 cut(s) 1156
XcmI CCANNNNNNNNNTGG 2 cut(s) 577, 1279
XhoI CTCGAG 3 cut(s) 278, 329, 1221
XmnI GAANNNNTTC 2 cut(s) 1395, 1482
XspI CTAG 3 cut(s) 854, 947, 1466
Zsp2I ATGCAT 1 cut(s) 1158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.