Rh3DG313400

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0006200
Physical Location & Seq
Reverse (-)
33666457 .. 33668132
1676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG313400.1

Sequence Viewer

Length: 1512 bp
ATGAATCGTAGCCGTTGGATGATGTCGTCGGGAGCGGGAGAGCTGGTGAGGAAGCTCTATACGGCGGCGGCGGAGGCGGGGGGTCGGCGTGACAATCCCGATAGGCTGTACCGGAGGTTATCGGCTTTGGGAGGAAGCGGGAGGAGCGCGGCCACGGCCTTGAACGATTACATCATGGAGGGCAGAACGGTCAAGAAATATGAGCTCGAGCGCTGCATCAAGGAGCTCCGCAAGTACGGCCAGTACCAGAATGCTCTAGAGATAATGGAATGGATGGAGTTTAGGAAAATTCATTATGCTTTGCCTGACTATGCCGTACGGTTGGATCTTACGGCGAAAGCCAAAGGGATAGAGGCAGCTGAGAGTTACTTCAGCAATTTAGCACCGTCTGCGAAGAACAAATTGACTTACGGATCCCTTTTGAACTGTTATTGCAAGGAAGTGATGGAAGAGAAGGCATTGGCGCTTTATCAGAAGATGGATGAGTTGGACTATGCTAACTCTACTCTGGTTTTTAACAATCTCATGTCTTTGTATATGAGAAAGGAGCGGCCGGAGAAAGTGGCGCCTTTCGTAGAGCAAATGAAGCAGAGGGAGATATCTTTAGATACGTTTAGTTACAATATCTGGATGCAGAGCTATGCCTCTTTGAATGATATTGAAGGCGTGGAGAGAGTTGTGGAAGAGATGCAAAGCCATGATCAAGACAAATGCAACTGGTCAACATATAGTAATTTGGCTTCCATCTATGTAAAGGCGCAGCTTTTTGAGAAGGCTGAGCTAGCGCTTAAAATGTCGGAGAAAATGATGATACCGCTGAAGCAGCGCCAGACATACCATTTCTTGATTACCCTCTACGCCAACACTGGTAATCTAGGTGAGGTCAAAAGAATATGGGAATCCCTCAAGTTAGCTTTCCCAGAAACCACCAATATGAGCTATATACTTATGCTTCAAGCTCTATGTAAGCTAAAAGATATTGAGGGTTTAAAAGAATGCTTTGAGGAGTGGCAGTCCAATTGTTCCAGTTATGATATGAGGTTAGCAAATGTAGTTATACGTGCTTATCTTAGTCAGGACATGTATGAAGAAGCGCTGTTGATCTTTAAGGATGCTACTAAGAGGTCTAAAGGGCCTTTCTTCAAGGCTCGAGAAATGTTCATGGTTTACTTCTTGGATGCCCGTCAGGTTGATTCGGCCATTAGCTATTTGGGAGAGGCAATTTCAGAAACCAAGGATGAGAATTGGCATCCGTCTCCAGAAATCATTGGTGCATTTTTTAAGTATTTTGAGGAAACAAAAGATGTTGATAGCTCTGAAAACTTTCATAAGATTCTGAAGCGTCTAAATTGTCTCAGTTCCAATGAGTATTCTTTGTTGCTTAAGGTTTATCTAGCTGCTGGTGAATTGCTTCCTGAGATATGTCAGAGATTGAAGGAAGACAACATTCAAATAAGCCCCGAGCTTGAGGAATTGGTGGAGAGGGTTTCCCCAAGTAGATCGACAGCATAG

Protein Analysis

503

Amino Acids

58.29

Weight (kDa)

5.86

Isoelectric Point (pI)

53.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 169 - 215 2.9e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 565
AccBSI CCGCTC 2 cut(s) 35, 550
AccII CGCG 1 cut(s) 149
AclWI GGATC 3 cut(s) 333, 408, 421
AcoI YGGCCR 4 cut(s) 150, 238, 551, 1197
AcsI RAATTY 1 cut(s) 288
AcuI CTGAAG 3 cut(s) 355, 839, 1358
AcyI GRCGYC 1 cut(s) 566
AfaI GTAC 4 cut(s) 110, 236, 245, 318
AfeI AGCGCT 3 cut(s) 212, 786, 1095
AflII CTTAAG 1 cut(s) 1382
AflIII ACRYGT 1 cut(s) 1080
AgsI TTSAA 8 cut(s) 163, 424, 652, 662, 956, 1144, 1435, 1451
Alw21I GWGCWC 2 cut(s) 207, 228
Alw26I GTCTC 2 cut(s) 1260, 1358
AlwI GGATC 3 cut(s) 333, 408, 421
Ama87I CYCGRG 3 cut(s) 206, 1149, 1460
Aor51HI AGCGCT 3 cut(s) 212, 786, 1095
AoxI GGCC 6 cut(s) 150, 156, 238, 551, 1133, 1197
ApeKI GCWGC 5 cut(s) 213, 356, 760, 823, 1397
ApoI RAATTY 1 cut(s) 288
Asp700I GAANNNNTTC 2 cut(s) 1323, 1410
AspLEI GCGC 8 cut(s) 149, 213, 466, 568, 760, 787, 828, 1096
AspS9I GGNCC 1 cut(s) 1133
AsuHPI GGTGA 3 cut(s) 58, 890, 1415
AsuNHI GCTAGC 1 cut(s) 781
AvaI CYCGRG 3 cut(s) 206, 1149, 1460
BamHI GGATCC 1 cut(s) 413
BanI GGYRCC 1 cut(s) 565
BanII GRGCYC 2 cut(s) 207, 228
BarI GAAGNNNNNNTAC 2 cut(s) 724, 756
BbsI GAAGAC 1 cut(s) 1446
Bbv12I GWGCWC 2 cut(s) 207, 228
BbvI GCAGC 5 cut(s) 200, 368, 772, 835, 1384
BccI CCATC 4 cut(s) 268, 439, 472, 752
BceAI ACGGC 5 cut(s) 78, 171, 253, 299, 348
BclI TGATCA 1 cut(s) 700
BcoDI GTCTC 2 cut(s) 1260, 1358
BfaI CTAG 4 cut(s) 257, 782, 875, 1394
BfoI RGCGCY 6 cut(s) 214, 467, 569, 788, 829, 1097
BfrI CTTAAG 1 cut(s) 1382
BisI GCNGC 9 cut(s) 66, 69, 150, 214, 357, 551, 761, 824, 1398
BlpI GCTNAGC 1 cut(s) 777
BlsI GCNGC 9 cut(s) 67, 70, 151, 215, 358, 552, 762, 825, 1399
BmeT110I CYCGRG 3 cut(s) 206, 1149, 1460
BmgT120I GGNCC 1 cut(s) 1133
BmiI GGNNCC 2 cut(s) 415, 567
BmsI GCATC 6 cut(s) 225, 621, 678, 1102, 1168, 1258
BmtI GCTAGC 1 cut(s) 785
BpiI GAAGAC 1 cut(s) 1446
BpmI CTGGAG 1 cut(s) 1242
Bpu1102I GCTNAGC 1 cut(s) 777
BpuEI CTTGAG 2 cut(s) 890, 1487
BsaAI YACGTR 1 cut(s) 1061
BsaHI GRCGYC 1 cut(s) 566
BsaJI CCNNGG 2 cut(s) 153, 1233
BsaWI WCCGGW 1 cut(s) 111
BsaXI ACNNNNNCTCC 4 cut(s) 30, 60, 998, 1028
Bse1I ACTGG 4 cut(s) 241, 722, 871, 1026
BseDI CCNNGG 2 cut(s) 153, 1233
BseGI GGATG 8 cut(s) 24, 279, 487, 636, 1117, 1183, 1243, 1249
BseMII CTCAG 4 cut(s) 351, 768, 1369, 1407
BseNI ACTGG 4 cut(s) 241, 722, 871, 1026
BseRI GAGGAG 2 cut(s) 157, 1019
BseX3I CGGCCG 1 cut(s) 551
BseXI GCAGC 5 cut(s) 200, 368, 772, 835, 1384
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 1 cut(s) 554
BshFI GGCC 6 cut(s) 152, 158, 240, 553, 1135, 1199
BshNI GGYRCC 1 cut(s) 565
BsiEI CGRYCG 1 cut(s) 554
BsiHKAI GWGCWC 2 cut(s) 207, 228
BsiHKCI CYCGRG 3 cut(s) 206, 1149, 1460
BsiSI CCGG 2 cut(s) 112, 554
BsiWI CGTACG 1 cut(s) 316
BsmAI GTCTC 2 cut(s) 1260, 1358
BsmBI CGTCTC 1 cut(s) 1260
BsmI GAATGC 2 cut(s) 256, 1001
BsnI GGCC 6 cut(s) 152, 158, 240, 553, 1135, 1199
BsoBI CYCGRG 3 cut(s) 206, 1149, 1460
Bsp1286I GDGCHC 2 cut(s) 207, 228
Bsp143I GATC 5 cut(s) 325, 413, 700, 1101, 1499
Bsp1720I GCTNAGC 1 cut(s) 777
BspANI GGCC 6 cut(s) 152, 158, 240, 553, 1135, 1199
BspCNI CTCAG 4 cut(s) 352, 769, 1368, 1408
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 2 cut(s) 415, 567
BspOI GCTAGC 1 cut(s) 785
BspPI GGATC 3 cut(s) 333, 408, 421
BspT107I GGYRCC 1 cut(s) 565
BspTI CTTAAG 1 cut(s) 1382
BsrBI CCGCTC 2 cut(s) 35, 550
BsrI ACTGG 4 cut(s) 241, 722, 871, 1026
BssECI CCNNGG 2 cut(s) 153, 1233
BssMI GATC 5 cut(s) 325, 413, 700, 1101, 1499
BssNI GRCGYC 1 cut(s) 566
BssT1I CCWWGG 1 cut(s) 1233
Bst4CI ACNGT 4 cut(s) 190, 321, 387, 428
Bst6I CTCTTC 2 cut(s) 444, 678
BstACI GRCGYC 1 cut(s) 566
BstAFI CTTAAG 1 cut(s) 1382
BstAPI GCANNNNNTGC 1 cut(s) 389
BstBAI YACGTR 1 cut(s) 1061
BstC8I GCNNGC 1 cut(s) 783
BstDEI CTNAG 6 cut(s) 360, 777, 1070, 1119, 1355, 1416
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 8 cut(s) 24, 279, 487, 636, 1117, 1183, 1243, 1249
BstFNI CGCG 1 cut(s) 149
BstH2I RGCGCY 6 cut(s) 214, 467, 569, 788, 829, 1097
BstHHI GCGC 8 cut(s) 149, 213, 466, 568, 760, 787, 828, 1096
BstKTI GATC 5 cut(s) 328, 416, 703, 1104, 1502
BstMAI GTCTC 2 cut(s) 1260, 1358
BstMBI GATC 5 cut(s) 325, 413, 700, 1101, 1499
BstMCI CGRYCG 1 cut(s) 554
BstMWI GCNNNNNNNGC 8 cut(s) 74, 144, 155, 237, 389, 586, 782, 823
BstNSI RCATGY 1 cut(s) 1084
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 5 cut(s) 200, 368, 772, 835, 1384
BstV2I GAAGAC 1 cut(s) 1446
BstX2I RGATCY 2 cut(s) 325, 413
BstYI RGATCY 2 cut(s) 325, 413
BstZI CGGCCG 1 cut(s) 551
BsuRI GGCC 6 cut(s) 152, 158, 240, 553, 1135, 1199
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 8 cut(s) 24, 279, 487, 636, 1117, 1183, 1243, 1249
BtsIMutI CAGTG 1 cut(s) 864
Cac8I GCNNGC 1 cut(s) 783
CfoI GCGC 8 cut(s) 149, 213, 466, 568, 760, 787, 828, 1096
Cfr13I GGNCC 1 cut(s) 1133
CseI GACGC 1 cut(s) 1331
Csp6I GTAC 4 cut(s) 109, 235, 244, 317
CviAII CATG 5 cut(s) 175, 526, 698, 1081, 1162
CviQI GTAC 4 cut(s) 109, 235, 244, 317
DdeI CTNAG 6 cut(s) 360, 777, 1070, 1119, 1355, 1416
DinI GGCGCC 1 cut(s) 567
DpnI GATC 5 cut(s) 327, 415, 702, 1103, 1501
DpnII GATC 5 cut(s) 325, 413, 700, 1101, 1499
DraI TTTAAA 1 cut(s) 990
EaeI YGGCCR 4 cut(s) 150, 238, 551, 1197
EagI CGGCCG 1 cut(s) 551
Eam1104I CTCTTC 2 cut(s) 444, 678
EarI CTCTTC 2 cut(s) 444, 678
EciI GGCGGA 1 cut(s) 86
Ecl136II GAGCTC 2 cut(s) 205, 226
EclXI CGGCCG 1 cut(s) 551
Eco130I CCWWGG 1 cut(s) 1233
Eco24I GRGCYC 2 cut(s) 207, 228
Eco32I GATATC 1 cut(s) 600
Eco47III AGCGCT 3 cut(s) 212, 786, 1095
Eco52I CGGCCG 1 cut(s) 551
Eco53kI GAGCTC 2 cut(s) 205, 226
Eco57I CTGAAG 3 cut(s) 355, 839, 1358
Eco88I CYCGRG 3 cut(s) 206, 1149, 1460
EcoICRI GAGCTC 2 cut(s) 205, 226
EcoO109I RGGNCCY 1 cut(s) 1133
EcoRV GATATC 1 cut(s) 600
EcoT14I CCWWGG 1 cut(s) 1233
EcoT38I GRGCYC 2 cut(s) 207, 228
EgeI GGCGCC 1 cut(s) 567
EheI GGCGCC 1 cut(s) 567
ErhI CCWWGG 1 cut(s) 1233
Esp3I CGTCTC 1 cut(s) 1260
FaeI CATG 5 cut(s) 178, 529, 701, 1084, 1165
FatI CATG 5 cut(s) 174, 525, 697, 1080, 1161
FauI CCCGC 3 cut(s) 28, 70, 131
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 9 cut(s) 66, 69, 150, 214, 357, 551, 761, 824, 1398
FokI GGATG 8 cut(s) 31, 286, 494, 643, 1124, 1190, 1236, 1250
FriOI GRGCYC 2 cut(s) 207, 228
Fsp4HI GCNGC 9 cut(s) 66, 69, 150, 214, 357, 551, 761, 824, 1398
FspBI CTAG 4 cut(s) 257, 782, 875, 1394
GlaI GCGC 8 cut(s) 148, 212, 465, 567, 759, 786, 827, 1095
GluI GCNGC 9 cut(s) 66, 69, 150, 214, 357, 551, 761, 824, 1398
GsuI CTGGAG 1 cut(s) 1242
HaeII RGCGCY 6 cut(s) 214, 467, 569, 788, 829, 1097
HaeIII GGCC 6 cut(s) 152, 158, 240, 553, 1135, 1199
HapII CCGG 2 cut(s) 112, 554
HgaI GACGC 1 cut(s) 1331
HhaI GCGC 8 cut(s) 149, 213, 466, 568, 760, 787, 828, 1096
Hin1I GRCGYC 1 cut(s) 566
Hin1II CATG 5 cut(s) 178, 529, 701, 1084, 1165
Hin6I GCGC 8 cut(s) 147, 211, 464, 566, 758, 785, 826, 1094
HinP1I GCGC 8 cut(s) 147, 211, 464, 566, 758, 785, 826, 1094
HincII GTYRAC 1 cut(s) 723
HindII GTYRAC 1 cut(s) 723
HinfI GANTC 4 cut(s) 4, 899, 1193, 1333
HpaII CCGG 2 cut(s) 112, 554
HphI GGTGA 3 cut(s) 58, 890, 1415
Hpy166II GTNNAC 2 cut(s) 723, 1168
Hpy188I TCNGA 6 cut(s) 474, 799, 1228, 1318, 1338, 1428
Hpy8I GTNNAC 2 cut(s) 723, 1168
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 4 cut(s) 448, 656, 766, 1429
HpyCH4III ACNGT 4 cut(s) 190, 321, 387, 428
HpyCH4IV ACGT 2 cut(s) 611, 1060
HpyCH4V TGCA 6 cut(s) 216, 435, 634, 691, 714, 1274
HpyF10VI GCNNNNNNNGC 8 cut(s) 74, 144, 155, 237, 389, 586, 782, 823
HpyF3I CTNAG 6 cut(s) 360, 777, 1070, 1119, 1355, 1416
HpySE526I ACGT 2 cut(s) 611, 1060
Hsp92I GRCGYC 1 cut(s) 566
Hsp92II CATG 5 cut(s) 178, 529, 701, 1084, 1165
HspAI GCGC 8 cut(s) 147, 211, 464, 566, 758, 785, 826, 1094
KasI GGCGCC 1 cut(s) 565
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 5 cut(s) 325, 413, 700, 1101, 1499
LmnI GCTCC 5 cut(s) 32, 144, 223, 231, 547
Lsp1109I GCAGC 5 cut(s) 200, 368, 772, 835, 1384
LweI GCATC 6 cut(s) 225, 621, 678, 1102, 1168, 1258
MaeI CTAG 4 cut(s) 257, 782, 875, 1394
MaeII ACGT 2 cut(s) 611, 1060
MaeIII GTNAC 3 cut(s) 89, 365, 617
MalI GATC 5 cut(s) 327, 415, 702, 1103, 1501
MbiI CCGCTC 2 cut(s) 35, 550
MboI GATC 5 cut(s) 325, 413, 700, 1101, 1499
MboII GAAGA 7 cut(s) 406, 461, 487, 695, 1100, 1132, 1451
MfeI CAATTG 1 cut(s) 1018
MflI RGATCY 2 cut(s) 325, 413
MhlI GDGCHC 2 cut(s) 207, 228
Mly113I GGCGCC 1 cut(s) 566
MmeI TCCRAC 3 cut(s) 303, 468, 777
MroXI GAANNNNTTC 2 cut(s) 1323, 1410
MseI TTAA 6 cut(s) 516, 789, 989, 1107, 1281, 1383
MslI CAYNNNNRTG 1 cut(s) 932
MspA1I CMGCKG 2 cut(s) 359, 817
MspCI CTTAAG 1 cut(s) 1382
MspI CCGG 2 cut(s) 112, 554
MunI CAATTG 1 cut(s) 1018
Mva1269I GAATGC 2 cut(s) 256, 1001
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 8 cut(s) 74, 144, 155, 237, 389, 586, 782, 823
NarI GGCGCC 1 cut(s) 566
NdeII GATC 5 cut(s) 325, 413, 700, 1101, 1499
NheI GCTAGC 1 cut(s) 781
NlaIII CATG 5 cut(s) 178, 529, 701, 1084, 1165
NlaIV GGNNCC 2 cut(s) 415, 567
NmuCI GTSAC 1 cut(s) 89
NspI RCATGY 1 cut(s) 1084
PaeR7I CTCGAG 2 cut(s) 206, 1149
PciI ACATGT 1 cut(s) 1080
PctI GAATGC 2 cut(s) 256, 1001
PdmI GAANNNNTTC 2 cut(s) 1323, 1410
PfeI GAWTC 4 cut(s) 4, 899, 1193, 1333
Pfl23II CGTACG 1 cut(s) 316
PkrI GCNGC 9 cut(s) 67, 70, 151, 215, 358, 552, 762, 825, 1399
PluTI GGCGCC 1 cut(s) 569
Ppu21I YACGTR 1 cut(s) 1061
PscI ACATGT 1 cut(s) 1080
Psp124BI GAGCTC 2 cut(s) 207, 228
PspLI CGTACG 1 cut(s) 316
PspN4I GGNNCC 2 cut(s) 415, 567
PspPI GGNCC 1 cut(s) 1133
PspXI VCTCGAGB 1 cut(s) 206
PsuI RGATCY 2 cut(s) 325, 413
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 4 cut(s) 110, 236, 245, 318
RsaNI GTAC 4 cut(s) 109, 235, 244, 317
RseI CAYNNNNRTG 1 cut(s) 932
SacI GAGCTC 2 cut(s) 207, 228
SaqAI TTAA 6 cut(s) 516, 789, 989, 1107, 1281, 1383
SatI GCNGC 9 cut(s) 66, 69, 150, 214, 357, 551, 761, 824, 1398
Sau3AI GATC 5 cut(s) 325, 413, 700, 1101, 1499
Sau96I GGNCC 1 cut(s) 1133
SduI GDGCHC 2 cut(s) 207, 228
SfaNI GCATC 6 cut(s) 225, 621, 678, 1102, 1168, 1258
SfoI GGCGCC 1 cut(s) 567
Sfr274I CTCGAG 2 cut(s) 206, 1149
SlaI CTCGAG 2 cut(s) 206, 1149
SmiMI CAYNNNNRTG 1 cut(s) 932
SmlI CTYRAG 5 cut(s) 206, 905, 1149, 1382, 1466
SmoI CTYRAG 5 cut(s) 206, 905, 1149, 1382, 1466
SspDI GGCGCC 1 cut(s) 565
SspMI CTAG 4 cut(s) 257, 782, 875, 1394
SstI GAGCTC 2 cut(s) 207, 228
StyI CCWWGG 1 cut(s) 1233
TaaI ACNGT 4 cut(s) 190, 321, 387, 428
TaiI ACGT 2 cut(s) 614, 1063
TaqI TCGA 3 cut(s) 207, 1150, 1502
TauI GCSGC 4 cut(s) 68, 71, 152, 553
TfiI GAWTC 4 cut(s) 4, 899, 1193, 1333
Tru1I TTAA 6 cut(s) 516, 789, 989, 1107, 1281, 1383
Tru9I TTAA 6 cut(s) 516, 789, 989, 1107, 1281, 1383
TscAI CASTG 1 cut(s) 871
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 5 cut(s) 213, 356, 760, 823, 1397
Tsp45I GTSAC 1 cut(s) 89
TspDTI ATGAA 6 cut(s) 17, 281, 599, 1101, 1150, 1316
TspGWI ACGGA 2 cut(s) 426, 1242
TspRI CASTG 1 cut(s) 871
Vha464I CTTAAG 1 cut(s) 1382
XapI RAATTY 1 cut(s) 288
XbaI TCTAGA 1 cut(s) 256
XceI RCATGY 1 cut(s) 1084
XcmI CCANNNNNNNNNTGG 1 cut(s) 1207
XhoI CTCGAG 2 cut(s) 206, 1149
XmnI GAANNNNTTC 2 cut(s) 1323, 1410
XspI CTAG 4 cut(s) 257, 782, 875, 1394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.