Rmu_sc0002136.1_g000003

TIC 22-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002136.1
OG0007361
Physical Location & Seq
Reverse (-)
12161 .. 18568
6408 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 969 bp
atgaacttgggcagcgccaataacccaaatgctccctcaccaccaccaccaccacctcaactcaacctccaacaagccttcacctccctccaaacccactgctctggtttcctccaccacctctctcatctccctctcttcaaccccaacgcttcttctctccaaacccacctccaatcatccctctccaacctccaaaaccaagccaaacacgcattccatgccgggttttcgggctttgattcctccaagcagaacccagtttgggctcgaattgctgtaaacaacaaaacccagttcccgaattctagtggcgatgggcccggagctgccatgtcgaccgaggccatcgaggagcggctggccggcgtgccggtctatgctctgagcaatgcggctgaggaattcgtgttggtttcgggtgcttcgagtgggaagagtcttggattgttttgtttcaagaaggaggacgcagagtcactgcttgagcatattagagttatggaccccggaatgcgaagcgggtccaaagttgtggctgtggctttgaataaggtttttcagctcaaggttgatggggtggccttcaggttgattcctgagtcttctcaagtcaagaatgcgcttaaggagaaggagaaggagaagggtggatcttctgataatggcttttttggggtgccagttttccagtcgaggagcttgattctaagaagccaaagcaagagctatcgtccagtatttttcaggaaggaggatttagaaaactcactatcaagagcttctcgtgagcagaatcaattaaatcctttacttagaccaggggacattcaggttgctgttctcgaggatgtaataaaggggatgaaggagggttctacatcggtctgggatgacgttgtttttatccctcctggatttgatatctctactgatcccaccaagcaacaggcagatgctgcaaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tic22

Protein Analysis

322

Amino Acids

34.85

Weight (kDa)

7.81

Isoelectric Point (pI)

54.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 60 - 312 1.2e-89 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 679
AccBSI CCGCTC 1 cut(s) 358
AccI GTMKAC 1 cut(s) 338
AciI CCGC 3 cut(s) 358, 395, 522
AclWI GGATC 2 cut(s) 661, 929
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 2 cut(s) 304, 404
AcuI CTGAAG 1 cut(s) 571
AfiI CCNNNNNNNGG 1 cut(s) 265
AflII CTTAAG 1 cut(s) 626
AgsI TTSAA 3 cut(s) 142, 460, 550
AhdI GACNNNNNGTC 1 cut(s) 475
AjnI CCWGG 2 cut(s) 820, 913
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 5 cut(s) 329, 565, 702, 729, 782
AluI AGCT 5 cut(s) 329, 565, 702, 729, 782
AlwI GGATC 2 cut(s) 661, 929
AlwNI CAGNNNCTG 1 cut(s) 959
Ama87I CYCGRG 1 cut(s) 845
AoxI GGCC 4 cut(s) 320, 345, 363, 582
ApaI GGGCCC 1 cut(s) 324
ApeKI GCWGC 3 cut(s) 12, 329, 959
ApoI RAATTY 2 cut(s) 304, 404
AspLEI GCGC 2 cut(s) 17, 625
AspS9I GGNCC 4 cut(s) 320, 321, 505, 525
AsuC2I CCSGG 3 cut(s) 226, 324, 510
AsuHPI GGTGA 2 cut(s) 30, 73
AvaI CYCGRG 1 cut(s) 845
AvaII GGWCC 2 cut(s) 505, 525
BaeGI GKGCMC 1 cut(s) 324
BanI GGYRCC 1 cut(s) 679
BanII GRGCYC 2 cut(s) 271, 324
BauI CACGAG 1 cut(s) 786
BbsI GAAGAC 1 cut(s) 597
BbvCI CCTCAGC 1 cut(s) 399
BbvI GCAGC 3 cut(s) 24, 316, 946
BccI CCATC 3 cut(s) 311, 356, 569
BciT130I CCWGG 2 cut(s) 822, 915
BcnI CCSGG 3 cut(s) 226, 324, 510
BfaI CTAG 1 cut(s) 309
BfoI RGCGCY 1 cut(s) 18
BfrI CTTAAG 1 cut(s) 626
BisI GCNGC 5 cut(s) 13, 330, 359, 396, 960
BlsI GCNGC 5 cut(s) 14, 331, 360, 397, 961
Bme1390I CCNGG 5 cut(s) 226, 324, 510, 822, 915
Bme18I GGWCC 2 cut(s) 505, 525
BmeRI GACNNNNNGTC 1 cut(s) 475
BmeT110I CYCGRG 1 cut(s) 845
BmgT120I GGNCC 4 cut(s) 320, 321, 505, 525
BmiI GGNNCC 4 cut(s) 322, 507, 526, 681
BmrFI CCNGG 5 cut(s) 226, 324, 510, 822, 915
BmrI ACTGGG 2 cut(s) 254, 289
BmsI GCATC 1 cut(s) 946
BmuI ACTGGG 2 cut(s) 254, 289
BpiI GAAGAC 1 cut(s) 597
Bpu10I CCTNAGC 1 cut(s) 399
BpuEI CTTGAG 3 cut(s) 506, 551, 594
BpuMI CCSGG 3 cut(s) 226, 324, 510
BsaJI CCNNGG 3 cut(s) 342, 508, 821
BsaXI ACNNNNNCTCC 2 cut(s) 51, 81
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse118I RCCGGY 2 cut(s) 365, 373
Bse1I ACTGG 5 cut(s) 260, 295, 683, 691, 737
Bse3DI GCAATG 1 cut(s) 397
BseBI CCWGG 2 cut(s) 822, 915
BseDI CCNNGG 3 cut(s) 342, 508, 821
BseGI GGATG 4 cut(s) 179, 856, 870, 898
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMI GCAATG 1 cut(s) 397
BseMII CTCAG 3 cut(s) 377, 390, 591
BseNI ACTGG 5 cut(s) 260, 295, 683, 691, 737
BseRI GAGGAG 2 cut(s) 368, 712
BseSI GKGCMC 1 cut(s) 324
BseXI GCAGC 3 cut(s) 24, 316, 946
Bsh1285I CGRYCG 1 cut(s) 342
BshFI GGCC 4 cut(s) 322, 347, 365, 584
BshNI GGYRCC 1 cut(s) 679
BsiEI CGRYCG 1 cut(s) 342
BsiHKCI CYCGRG 1 cut(s) 845
BsiSI CCGG 5 cut(s) 225, 324, 366, 374, 510
BslFI GGGAC 1 cut(s) 839
BslI CCNNNNNNNGG 1 cut(s) 265
BsmFI GGGAC 1 cut(s) 839
BsmI GAATGC 3 cut(s) 215, 519, 625
BsnI GGCC 4 cut(s) 322, 347, 365, 584
BsoBI CYCGRG 1 cut(s) 845
Bsp120I GGGCCC 1 cut(s) 320
Bsp1286I GDGCHC 2 cut(s) 271, 324
Bsp143I GATC 2 cut(s) 653, 934
BspACI CCGC 3 cut(s) 358, 395, 522
BspANI GGCC 4 cut(s) 322, 347, 365, 584
BspCNI CTCAG 3 cut(s) 378, 391, 592
BspLI GGNNCC 4 cut(s) 322, 507, 526, 681
BspPI GGATC 2 cut(s) 661, 929
BspT107I GGYRCC 1 cut(s) 679
BspTI CTTAAG 1 cut(s) 626
BsrBI CCGCTC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 397
BsrFI RCCGGY 2 cut(s) 365, 373
BsrI ACTGG 5 cut(s) 260, 295, 683, 691, 737
BssAI RCCGGY 2 cut(s) 365, 373
BssECI CCNNGG 3 cut(s) 342, 508, 821
BssMI GATC 2 cut(s) 653, 934
BssSI CACGAG 1 cut(s) 786
Bst2BI CACGAG 1 cut(s) 786
Bst2UI CCWGG 2 cut(s) 822, 915
Bst6I CTCTTC 2 cut(s) 143, 431
BstAFI CTTAAG 1 cut(s) 626
BstAPI GCANNNNNTGC 2 cut(s) 221, 959
BstC8I GCNNGC 3 cut(s) 363, 367, 371
BstDEI CTNAG 5 cut(s) 386, 399, 600, 710, 815
BstF5I GGATG 4 cut(s) 179, 856, 870, 898
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 2 cut(s) 17, 625
BstKTI GATC 2 cut(s) 656, 937
BstMBI GATC 2 cut(s) 653, 934
BstMCI CGRYCG 1 cut(s) 342
BstMWI GCNNNNNNNGC 4 cut(s) 212, 221, 275, 959
BstNI CCWGG 2 cut(s) 822, 915
BstSCI CCNGG 5 cut(s) 224, 322, 508, 820, 913
BstSLI GKGCMC 1 cut(s) 324
BstV1I GCAGC 3 cut(s) 24, 316, 946
BstV2I GAAGAC 1 cut(s) 597
BstX2I RGATCY 1 cut(s) 653
BstXI CCANNNNNNTGG 2 cut(s) 104, 535
BstYI RGATCY 1 cut(s) 653
BsuRI GGCC 4 cut(s) 322, 347, 365, 584
BtgZI GCGATG 1 cut(s) 330
BtsCI GGATG 4 cut(s) 179, 856, 870, 898
BtsI GCAGTG 2 cut(s) 97, 479
BtsIMutI CAGTG 2 cut(s) 97, 479
Cac8I GCNNGC 3 cut(s) 363, 367, 371
CaiI CAGNNNCTG 1 cut(s) 959
CfoI GCGC 2 cut(s) 17, 625
Cfr10I RCCGGY 2 cut(s) 365, 373
Cfr13I GGNCC 4 cut(s) 320, 321, 505, 525
CseI GACGC 1 cut(s) 479
CviAII CATG 2 cut(s) 221, 334
DdeI CTNAG 5 cut(s) 386, 399, 600, 710, 815
DpnI GATC 2 cut(s) 655, 936
DpnII GATC 2 cut(s) 653, 934
DriI GACNNNNNGTC 1 cut(s) 475
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 2 cut(s) 143, 431
Eam1105I GACNNNNNGTC 1 cut(s) 475
EarI CTCTTC 2 cut(s) 143, 431
Eco24I GRGCYC 2 cut(s) 271, 324
Eco32I GATATC 1 cut(s) 925
Eco47I GGWCC 2 cut(s) 505, 525
Eco57I CTGAAG 1 cut(s) 571
Eco88I CYCGRG 1 cut(s) 845
EcoRI GAATTC 2 cut(s) 304, 404
EcoRII CCWGG 2 cut(s) 820, 913
EcoRV GATATC 1 cut(s) 925
EcoT38I GRGCYC 2 cut(s) 271, 324
FaeI CATG 2 cut(s) 224, 337
FaiI YATR 5 cut(s) 222, 335, 381, 492, 503
FaqI GGGAC 1 cut(s) 839
FatI CATG 2 cut(s) 220, 333
FauI CCCGC 1 cut(s) 515
FblI GTMKAC 1 cut(s) 338
Fnu4HI GCNGC 5 cut(s) 13, 330, 359, 396, 960
FokI GGATG 4 cut(s) 166, 863, 877, 905
FriOI GRGCYC 2 cut(s) 271, 324
Fsp4HI GCNGC 5 cut(s) 13, 330, 359, 396, 960
FspBI CTAG 1 cut(s) 309
GlaI GCGC 2 cut(s) 16, 624
GluI GCNGC 5 cut(s) 13, 330, 359, 396, 960
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 4 cut(s) 322, 347, 365, 584
HapII CCGG 5 cut(s) 225, 324, 366, 374, 510
HgaI GACGC 1 cut(s) 479
HhaI GCGC 2 cut(s) 17, 625
Hin1II CATG 2 cut(s) 224, 337
Hin6I GCGC 2 cut(s) 15, 623
HinP1I GCGC 2 cut(s) 15, 623
HincII GTYRAC 1 cut(s) 339
HindII GTYRAC 1 cut(s) 339
HinfI GANTC 7 cut(s) 242, 439, 476, 595, 602, 706, 796
HpaII CCGG 5 cut(s) 225, 324, 366, 374, 510
HphI GGTGA 2 cut(s) 30, 73
Hpy166II GTNNAC 2 cut(s) 283, 339
Hpy188I TCNGA 2 cut(s) 387, 661
Hpy188III TCNNGA 8 cut(s) 301, 460, 599, 616, 748, 777, 788, 845
Hpy8I GTNNAC 2 cut(s) 283, 339
HpyAV CCTTC 8 cut(s) 88, 457, 595, 628, 634, 640, 745, 862
HpyCH4IV ACGT 1 cut(s) 897
HpyCH4V TGCA 1 cut(s) 962
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 221, 275, 959
HpyF3I CTNAG 5 cut(s) 386, 399, 600, 710, 815
HpySE526I ACGT 1 cut(s) 897
Hsp92II CATG 2 cut(s) 224, 337
HspAI GCGC 2 cut(s) 15, 623
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
Kzo9I GATC 2 cut(s) 653, 934
LmnI GCTCC 4 cut(s) 37, 326, 355, 699
Lsp1109I GCAGC 3 cut(s) 24, 316, 946
LweI GCATC 1 cut(s) 946
MaeI CTAG 1 cut(s) 309
MaeII ACGT 1 cut(s) 897
MaeIII GTNAC 1 cut(s) 477
MalI GATC 2 cut(s) 655, 936
MbiI CCGCTC 1 cut(s) 358
MboI GATC 2 cut(s) 653, 934
MboII GAAGA 5 cut(s) 130, 147, 448, 597, 648
MflI RGATCY 1 cut(s) 653
MhlI GDGCHC 2 cut(s) 271, 324
MluCI AATT 4 cut(s) 273, 304, 404, 800
MlyI GAGTC 3 cut(s) 448, 485, 611
MmeI TCCRAC 2 cut(s) 94, 213
MroNI GCCGGC 1 cut(s) 365
MseI TTAA 2 cut(s) 627, 803
MspCI CTTAAG 1 cut(s) 626
MspI CCGG 5 cut(s) 225, 324, 366, 374, 510
MspR9I CCNGG 5 cut(s) 226, 324, 510, 822, 915
Mva1269I GAATGC 3 cut(s) 215, 519, 625
MvaI CCWGG 2 cut(s) 822, 915
MwoI GCNNNNNNNGC 4 cut(s) 212, 221, 275, 959
NaeI GCCGGC 1 cut(s) 367
NciI CCSGG 3 cut(s) 226, 324, 510
NdeII GATC 2 cut(s) 653, 934
NgoMIV GCCGGC 1 cut(s) 365
NlaIII CATG 2 cut(s) 224, 337
NlaIV GGNNCC 4 cut(s) 322, 507, 526, 681
NmuCI GTSAC 1 cut(s) 477
PaeR7I CTCGAG 1 cut(s) 845
PcsI WCGNNNNNNNCGW 1 cut(s) 425
PctI GAATGC 3 cut(s) 215, 519, 625
PdiI GCCGGC 1 cut(s) 367
PfeI GAWTC 4 cut(s) 242, 595, 706, 796
PfoI TCCNGGA 1 cut(s) 913
PkrI GCNGC 5 cut(s) 14, 331, 360, 397, 961
PleI GAGTC 3 cut(s) 447, 484, 610
PpsI GAGTC 3 cut(s) 447, 484, 610
Psp6I CCWGG 2 cut(s) 820, 913
PspGI CCWGG 2 cut(s) 820, 913
PspN4I GGNNCC 4 cut(s) 322, 507, 526, 681
PspOMI GGGCCC 1 cut(s) 320
PspPI GGNCC 4 cut(s) 320, 321, 505, 525
PstNI CAGNNNCTG 1 cut(s) 959
PsuI RGATCY 1 cut(s) 653
SalI GTCGAC 1 cut(s) 337
SaqAI TTAA 2 cut(s) 627, 803
SatI GCNGC 5 cut(s) 13, 330, 359, 396, 960
Sau3AI GATC 2 cut(s) 653, 934
Sau96I GGNCC 4 cut(s) 320, 321, 505, 525
SchI GAGTC 3 cut(s) 448, 485, 611
ScrFI CCNGG 5 cut(s) 226, 324, 510, 822, 915
SduI GDGCHC 2 cut(s) 271, 324
SfaNI GCATC 1 cut(s) 946
Sfr274I CTCGAG 1 cut(s) 845
SinI GGWCC 2 cut(s) 505, 525
SlaI CTCGAG 1 cut(s) 845
SmlI CTYRAG 5 cut(s) 485, 566, 609, 626, 845
SmoI CTYRAG 5 cut(s) 485, 566, 609, 626, 845
Sse9I AATT 4 cut(s) 273, 304, 404, 800
SsiI CCGC 3 cut(s) 358, 395, 522
SspMI CTAG 1 cut(s) 309
StyD4I CCNGG 5 cut(s) 224, 322, 508, 820, 913
TaiI ACGT 1 cut(s) 900
TaqI TCGA 6 cut(s) 271, 338, 351, 428, 695, 846
TaqII GACCGA 2 cut(s) 356, 874
TasI AATT 4 cut(s) 273, 304, 404, 800
TauI GCSGC 2 cut(s) 361, 398
TfiI GAWTC 4 cut(s) 242, 595, 706, 796
Tru1I TTAA 2 cut(s) 627, 803
Tru9I TTAA 2 cut(s) 627, 803
TscAI CASTG 2 cut(s) 104, 486
TseFI GTSAC 1 cut(s) 477
TseI GCWGC 3 cut(s) 12, 329, 959
Tsp45I GTSAC 1 cut(s) 477
TspDTI ATGAA 2 cut(s) 17, 881
TspRI CASTG 2 cut(s) 104, 486
Vha464I CTTAAG 1 cut(s) 626
VpaK11BI GGWCC 2 cut(s) 505, 525
XapI RAATTY 2 cut(s) 304, 404
XhoI CTCGAG 1 cut(s) 845
XmiI GTMKAC 1 cut(s) 338
XspI CTAG 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.