Rmu_sc0002219.1_g000021

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002219.1
OG0015768
Physical Location & Seq
Reverse (-)
76936 .. 78323
1388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002219.1_g000021.1.cds

Sequence Viewer

Length: 1254 bp
atggctggctcaatggcgatggcgcagggttcggccacggtatcactgggatctgtggggcttgaggctctgaaggcggaccgttggtacatggtggggcgtttacttggacccaaagccatgtttcatggcttcaaaggaacgatctcttcaatctggaggcttcattcggggttaacgatccaagatgtgggggatcgttttgtgtttcaatttgatcgagaagctgatcgcaacaaaatcttgcatggaggcccgtggttttttaggaacaccatgctggtgttgggtgggtatgatggtatagggccggtggcggaagtctctcttaatcttctggagacctgggttgttgtcaagggtctcccactggtgctccggaataagaaggcgctagggttggttggggcgtctgtcggtcgtgttatcagttttgatcagacggcccttacccggaaggaagaggagcaacgtatccgccttgtacttgagtgcgtgacaggattcggggaaaaagaagagatctgcgcgcaactctcggcggcggctatggctggactctctctcatgtcagaagggcaaggaaggagggagaacaatagagtttcgatgtccggtagtagcacgggactggtggaggaggtgttgcccttgatgggtgatggggtggtatcggaatcgctagaagctgaaatggtggtagtccaaccgccacaggaagtccatgcatcacttccctccaattttcttggagccataagagctattgaaaaggcttttgcaggttttaatatggctgagtttctccagaattgggctctgcagagtaacaagagtaagaggcccgggaatggcaacagggagattccaccgttggtggtgactcaccctttgtgctcgcttggtggtcaggaagtgatggtgctgcaagactctcgggtgcagcctctactcgggactaaaaggaaggcggtggatactttgggcagagttggcaagcgatttgtcgacacctctactcccctgctcaaggtcgatgatgaattgcagttggtactccaacataaaaatggtcagctcgtggtttctccacggaagaagaaagtggggcgtccaaaggggagcaagaataagcccaagtgtgcgagacttcttgataaggagatgcacggtaaaaagcagagaaaatggaaagcacgtgaggaaggggagggtgctcgactaccatcggtagctgctgaagttacgaactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

417

Amino Acids

45.44

Weight (kDa)

9.65

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 773
AccB7I CCANNNNNTGG 1 cut(s) 190
AccI GTMKAC 1 cut(s) 1008
AccII CGCG 1 cut(s) 530
AccIII TCCGGA 1 cut(s) 378
AciI CCGC 7 cut(s) 77, 317, 478, 542, 545, 710, 971
AclWI GGATC 3 cut(s) 58, 175, 204
AcoI YGGCCR 1 cut(s) 33
AcuI CTGAAG 1 cut(s) 92
AcvI CACGTG 1 cut(s) 1199
AcyI GRCGYC 2 cut(s) 410, 1111
AfaI GTAC 3 cut(s) 89, 486, 1056
AfiI CCNNNNNNNGG 7 cut(s) 190, 453, 656, 814, 851, 953, 1030
AgsI TTSAA 4 cut(s) 136, 153, 212, 770
AjnI CCWGG 1 cut(s) 344
AluBI AGCT 5 cut(s) 227, 689, 764, 1078, 1235
AluI AGCT 5 cut(s) 227, 689, 764, 1078, 1235
Alw21I GWGCWC 3 cut(s) 378, 899, 1219
Alw26I GTCTC 4 cut(s) 328, 335, 368, 1141
AlwI GGATC 3 cut(s) 58, 175, 204
Ama87I CYCGRG 3 cut(s) 845, 936, 953
Aor13HI TCCGGA 1 cut(s) 378
AoxI GGCC 5 cut(s) 33, 253, 308, 444, 842
ApeKI GCWGC 3 cut(s) 925, 943, 1235
ArsI GACNNNNNNTTYG 2 cut(s) 874, 906
AspLEI GCGC 4 cut(s) 25, 394, 530, 532
AspS9I GGNCC 6 cut(s) 79, 110, 254, 308, 445, 843
AsuC2I CCSGG 3 cut(s) 454, 846, 847
AsuHPI GGTGA 3 cut(s) 671, 878, 892
AvaI CYCGRG 3 cut(s) 845, 936, 953
AvaII GGWCC 2 cut(s) 79, 110
BanII GRGCYC 1 cut(s) 820
BauI CACGAG 1 cut(s) 1079
BbrPI CACGTG 1 cut(s) 1199
Bbv12I GWGCWC 3 cut(s) 378, 899, 1219
BbvI GCAGC 3 cut(s) 912, 955, 1222
BccI CCATC 6 cut(s) 13, 293, 649, 656, 913, 1234
BceAI ACGGC 1 cut(s) 459
BcgI CGANNNNNNTGC 2 cut(s) 917, 951
BciT130I CCWGG 1 cut(s) 346
BciVI GTATCC 2 cut(s) 485, 970
BclI TGATCA 1 cut(s) 436
BcnI CCSGG 3 cut(s) 454, 846, 847
BcoDI GTCTC 4 cut(s) 328, 335, 368, 1141
BfaI CTAG 3 cut(s) 395, 683, 1252
BfmI CTRYAG 1 cut(s) 821
BfoI RGCGCY 1 cut(s) 395
BfuAI ACCTGC 1 cut(s) 773
BfuI GTATCC 2 cut(s) 485, 970
BglII AGATCT 1 cut(s) 522
BisI GCNGC 5 cut(s) 543, 546, 926, 944, 1236
BlsI GCNGC 5 cut(s) 544, 547, 927, 945, 1237
Bme1390I CCNGG 4 cut(s) 346, 454, 846, 847
Bme18I GGWCC 2 cut(s) 79, 110
BmeT110I CYCGRG 3 cut(s) 845, 936, 953
BmgT120I GGNCC 6 cut(s) 79, 110, 254, 308, 445, 843
BmiI GGNNCC 2 cut(s) 112, 754
BmrFI CCNGG 4 cut(s) 346, 454, 846, 847
BmrI ACTGGG 1 cut(s) 56
BmsI GCATC 2 cut(s) 737, 1155
BmuI ACTGGG 1 cut(s) 56
BpmI CTGGAG 3 cut(s) 178, 359, 791
BpuEI CTTGAG 3 cut(s) 83, 509, 1013
BpuMI CCSGG 3 cut(s) 454, 846, 847
BsaAI YACGTR 1 cut(s) 1199
BsaHI GRCGYC 2 cut(s) 410, 1111
BsaI GGTCTC 2 cut(s) 335, 368
BsaJI CCNNGG 5 cut(s) 36, 257, 345, 845, 1091
BsaWI WCCGGW 2 cut(s) 378, 614
BsaXI ACNNNNNCTCC 6 cut(s) 360, 390, 629, 659, 1003, 1033
Bsc4I CCNNNNNNNGG 7 cut(s) 190, 453, 656, 814, 851, 953, 1030
Bse118I RCCGGY 1 cut(s) 310
Bse1I ACTGG 3 cut(s) 51, 375, 636
BseAI TCCGGA 1 cut(s) 378
BseBI CCWGG 1 cut(s) 346
BseDI CCNNGG 5 cut(s) 36, 257, 345, 845, 1091
BseLI CCNNNNNNNGG 7 cut(s) 190, 453, 656, 814, 851, 953, 1030
BseMII CTCAG 1 cut(s) 789
BseNI ACTGG 3 cut(s) 51, 375, 636
BsePI GCGCGC 1 cut(s) 528
BseRI GAGGAG 2 cut(s) 479, 653
BseXI GCAGC 3 cut(s) 912, 955, 1222
BsgI GTGCAG 1 cut(s) 962
Bsh1236I CGCG 1 cut(s) 530
Bsh1285I CGRYCG 1 cut(s) 421
BshFI GGCC 5 cut(s) 35, 255, 310, 446, 844
BsiEI CGRYCG 1 cut(s) 421
BsiHKAI GWGCWC 3 cut(s) 378, 899, 1219
BsiHKCI CYCGRG 3 cut(s) 845, 936, 953
BsiSI CCGG 5 cut(s) 311, 379, 454, 615, 846
BslFI GGGAC 2 cut(s) 642, 970
BslI CCNNNNNNNGG 7 cut(s) 190, 453, 656, 814, 851, 953, 1030
BsmAI GTCTC 4 cut(s) 328, 335, 368, 1141
BsmFI GGGAC 2 cut(s) 642, 970
BsnI GGCC 5 cut(s) 35, 255, 310, 446, 844
Bso31I GGTCTC 2 cut(s) 335, 368
BsoBI CYCGRG 3 cut(s) 845, 936, 953
Bsp1286I GDGCHC 4 cut(s) 378, 820, 899, 1219
Bsp13I TCCGGA 1 cut(s) 378
Bsp143I GATC 8 cut(s) 50, 144, 180, 196, 217, 229, 436, 522
BspACI CCGC 7 cut(s) 77, 317, 478, 542, 545, 710, 971
BspANI GGCC 5 cut(s) 35, 255, 310, 446, 844
BspCNI CTCAG 1 cut(s) 790
BspEI TCCGGA 1 cut(s) 378
BspFNI CGCG 1 cut(s) 530
BspLI GGNNCC 2 cut(s) 112, 754
BspMAI CTGCAG 1 cut(s) 825
BspMI ACCTGC 1 cut(s) 773
BspPI GGATC 3 cut(s) 58, 175, 204
BspTNI GGTCTC 2 cut(s) 335, 368
BsrFI RCCGGY 1 cut(s) 310
BsrI ACTGG 3 cut(s) 51, 375, 636
BssAI RCCGGY 1 cut(s) 310
BssECI CCNNGG 5 cut(s) 36, 257, 345, 845, 1091
BssHII GCGCGC 1 cut(s) 528
BssMI GATC 8 cut(s) 50, 144, 180, 196, 217, 229, 436, 522
BssNI GRCGYC 2 cut(s) 410, 1111
BssSI CACGAG 1 cut(s) 1079
Bst2BI CACGAG 1 cut(s) 1079
Bst2UI CCWGG 1 cut(s) 346
Bst4CI ACNGT 4 cut(s) 40, 83, 873, 1172
Bst6I CTCTTC 3 cut(s) 154, 456, 513
BstACI GRCGYC 2 cut(s) 410, 1111
BstBAI YACGTR 1 cut(s) 1199
BstC8I GCNNGC 4 cut(s) 7, 530, 899, 998
BstDEI CTNAG 1 cut(s) 798
BstDSI CCRYGG 3 cut(s) 36, 257, 1091
BstENI CCTNNNNNAGG 1 cut(s) 1028
BstFNI CGCG 1 cut(s) 530
BstH2I RGCGCY 1 cut(s) 395
BstHHI GCGC 4 cut(s) 25, 394, 530, 532
BstKTI GATC 8 cut(s) 53, 147, 183, 199, 220, 232, 439, 525
BstMAI GTCTC 4 cut(s) 328, 335, 368, 1141
BstMBI GATC 8 cut(s) 50, 144, 180, 196, 217, 229, 436, 522
BstMCI CGRYCG 1 cut(s) 421
BstMWI GCNNNNNNNGC 4 cut(s) 74, 551, 761, 993
BstNI CCWGG 1 cut(s) 346
BstSCI CCNGG 4 cut(s) 344, 452, 844, 845
BstSFI CTRYAG 1 cut(s) 821
BstUI CGCG 1 cut(s) 530
BstV1I GCAGC 3 cut(s) 912, 955, 1222
BstX2I RGATCY 2 cut(s) 50, 522
BstYI RGATCY 2 cut(s) 50, 522
BsuI GTATCC 2 cut(s) 485, 970
BsuRI GGCC 5 cut(s) 35, 255, 310, 446, 844
BtgI CCRYGG 3 cut(s) 36, 257, 1091
BtgZI GCGATG 1 cut(s) 32
BtsIMutI CAGTG 2 cut(s) 44, 368
BveI ACCTGC 1 cut(s) 773
Cac8I GCNNGC 4 cut(s) 7, 530, 899, 998
CfoI GCGC 4 cut(s) 25, 394, 530, 532
Cfr10I RCCGGY 1 cut(s) 310
Cfr13I GGNCC 6 cut(s) 79, 110, 254, 308, 445, 843
Cfr9I CCCGGG 1 cut(s) 845
CpoI CGGWCCG 1 cut(s) 79
CseI GACGC 2 cut(s) 399, 1100
Csp6I GTAC 3 cut(s) 88, 485, 1055
CspI CGGWCCG 1 cut(s) 79
CviAII CATG 7 cut(s) 91, 121, 128, 248, 277, 568, 725
CviQI GTAC 3 cut(s) 88, 485, 1055
DdeI CTNAG 1 cut(s) 798
DpnI GATC 8 cut(s) 52, 146, 182, 198, 219, 231, 438, 524
DpnII GATC 8 cut(s) 50, 144, 180, 196, 217, 229, 436, 522
EaeI YGGCCR 1 cut(s) 33
Eam1104I CTCTTC 3 cut(s) 154, 456, 513
EarI CTCTTC 3 cut(s) 154, 456, 513
EciI GGCGGA 3 cut(s) 92, 332, 467
Eco24I GRGCYC 1 cut(s) 820
Eco31I GGTCTC 2 cut(s) 335, 368
Eco47I GGWCC 2 cut(s) 79, 110
Eco57I CTGAAG 1 cut(s) 92
Eco72I CACGTG 1 cut(s) 1199
Eco88I CYCGRG 3 cut(s) 845, 936, 953
EcoNI CCTNNNNNAGG 1 cut(s) 1028
EcoRII CCWGG 1 cut(s) 344
EcoT22I ATGCAT 1 cut(s) 730
EcoT38I GRGCYC 1 cut(s) 820
FaeI CATG 7 cut(s) 94, 124, 131, 251, 280, 571, 728
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FaqI GGGAC 2 cut(s) 642, 970
FatI CATG 7 cut(s) 90, 120, 127, 247, 276, 567, 724
FbaI TGATCA 1 cut(s) 436
FblI GTMKAC 1 cut(s) 1008
Fnu4HI GCNGC 5 cut(s) 543, 546, 926, 944, 1236
FriOI GRGCYC 1 cut(s) 820
Fsp4HI GCNGC 5 cut(s) 543, 546, 926, 944, 1236
FspBI CTAG 3 cut(s) 395, 683, 1252
GlaI GCGC 4 cut(s) 24, 393, 529, 531
GluI GCNGC 5 cut(s) 543, 546, 926, 944, 1236
GsuI CTGGAG 3 cut(s) 178, 359, 791
HaeII RGCGCY 1 cut(s) 395
HaeIII GGCC 5 cut(s) 35, 255, 310, 446, 844
HapII CCGG 5 cut(s) 311, 379, 454, 615, 846
HgaI GACGC 2 cut(s) 399, 1100
HhaI GCGC 4 cut(s) 25, 394, 530, 532
Hin1I GRCGYC 2 cut(s) 410, 1111
Hin1II CATG 7 cut(s) 94, 124, 131, 251, 280, 571, 728
Hin6I GCGC 4 cut(s) 23, 392, 528, 530
HinP1I GCGC 4 cut(s) 23, 392, 528, 530
HincII GTYRAC 2 cut(s) 177, 1009
HindII GTYRAC 2 cut(s) 177, 1009
HinfI GANTC 6 cut(s) 504, 558, 677, 865, 883, 932
HpaI GTTAAC 1 cut(s) 177
HpaII CCGG 5 cut(s) 311, 379, 454, 615, 846
HphI GGTGA 3 cut(s) 671, 878, 892
Hpy166II GTNNAC 3 cut(s) 104, 177, 1009
Hpy188I TCNGA 4 cut(s) 72, 441, 574, 676
Hpy188III TCNNGA 8 cut(s) 157, 221, 338, 379, 808, 911, 955, 1154
Hpy8I GTNNAC 3 cut(s) 104, 177, 1009
HpyAV CCTTC 7 cut(s) 67, 382, 451, 569, 579, 961, 1199
HpyCH4III ACNGT 4 cut(s) 40, 83, 873, 1172
HpyCH4IV ACGT 2 cut(s) 472, 1198
HpyCH4V TGCA 8 cut(s) 247, 728, 782, 823, 928, 943, 1048, 1168
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 551, 761, 993
HpyF3I CTNAG 1 cut(s) 798
HpySE526I ACGT 2 cut(s) 472, 1198
Hsp92I GRCGYC 2 cut(s) 410, 1111
Hsp92II CATG 7 cut(s) 94, 124, 131, 251, 280, 571, 728
HspAI GCGC 4 cut(s) 23, 392, 528, 530
Kpn2I TCCGGA 1 cut(s) 378
Ksp22I TGATCA 1 cut(s) 436
KspAI GTTAAC 1 cut(s) 177
Kzo9I GATC 8 cut(s) 50, 144, 180, 196, 217, 229, 436, 522
LmnI GCTCC 4 cut(s) 381, 466, 752, 1122
Lsp1109I GCAGC 3 cut(s) 912, 955, 1222
LweI GCATC 2 cut(s) 737, 1155
MaeI CTAG 3 cut(s) 395, 683, 1252
MaeII ACGT 2 cut(s) 472, 1198
MaeIII GTNAC 4 cut(s) 496, 827, 880, 1243
MalI GATC 8 cut(s) 52, 146, 182, 198, 219, 231, 438, 524
MboI GATC 8 cut(s) 50, 144, 180, 196, 217, 229, 436, 522
MboII GAAGA 6 cut(s) 141, 326, 473, 530, 1108, 1111
MflI RGATCY 2 cut(s) 50, 522
MhlI GDGCHC 4 cut(s) 378, 820, 899, 1219
MluCI AATT 4 cut(s) 212, 742, 811, 1043
MlyI GAGTC 3 cut(s) 552, 877, 926
MmeI TCCRAC 2 cut(s) 730, 1084
Mph1103I ATGCAT 1 cut(s) 730
MroI TCCGGA 1 cut(s) 378
MseI TTAA 3 cut(s) 176, 330, 789
MslI CAYNNNNRTG 2 cut(s) 281, 1068
MspI CCGG 5 cut(s) 311, 379, 454, 615, 846
MspR9I CCNGG 4 cut(s) 346, 454, 846, 847
MvaI CCWGG 1 cut(s) 346
MvnI CGCG 1 cut(s) 530
MwoI GCNNNNNNNGC 4 cut(s) 74, 551, 761, 993
NciI CCSGG 3 cut(s) 454, 846, 847
NdeII GATC 8 cut(s) 50, 144, 180, 196, 217, 229, 436, 522
NlaIII CATG 7 cut(s) 94, 124, 131, 251, 280, 571, 728
NlaIV GGNNCC 2 cut(s) 112, 754
NmeAIII GCCGAG 1 cut(s) 518
NmuCI GTSAC 2 cut(s) 496, 880
NsiI ATGCAT 1 cut(s) 730
PauI GCGCGC 1 cut(s) 528
PcsI WCGNNNNNNNCGW 1 cut(s) 176
PfeI GAWTC 3 cut(s) 504, 677, 865
PflMI CCANNNNNTGG 1 cut(s) 190
PkrI GCNGC 5 cut(s) 544, 547, 927, 945, 1237
PleI GAGTC 3 cut(s) 552, 877, 926
PmaCI CACGTG 1 cut(s) 1199
PmlI CACGTG 1 cut(s) 1199
PpsI GAGTC 3 cut(s) 552, 877, 926
Ppu21I YACGTR 1 cut(s) 1199
Psp6I CCWGG 1 cut(s) 344
PspCI CACGTG 1 cut(s) 1199
PspGI CCWGG 1 cut(s) 344
PspN4I GGNNCC 2 cut(s) 112, 754
PspPI GGNCC 6 cut(s) 79, 110, 254, 308, 445, 843
PstI CTGCAG 1 cut(s) 825
PsuI RGATCY 2 cut(s) 50, 522
PteI GCGCGC 1 cut(s) 528
RsaI GTAC 3 cut(s) 89, 486, 1056
RsaNI GTAC 3 cut(s) 88, 485, 1055
RseI CAYNNNNRTG 2 cut(s) 281, 1068
Rsr2I CGGWCCG 1 cut(s) 79
RsrII CGGWCCG 1 cut(s) 79
SalI GTCGAC 1 cut(s) 1007
SaqAI TTAA 3 cut(s) 176, 330, 789
SatI GCNGC 5 cut(s) 543, 546, 926, 944, 1236
Sau3AI GATC 8 cut(s) 50, 144, 180, 196, 217, 229, 436, 522
Sau96I GGNCC 6 cut(s) 79, 110, 254, 308, 445, 843
SchI GAGTC 3 cut(s) 552, 877, 926
ScrFI CCNGG 4 cut(s) 346, 454, 846, 847
SduI GDGCHC 4 cut(s) 378, 820, 899, 1219
SfaNI GCATC 2 cut(s) 737, 1155
SfcI CTRYAG 1 cut(s) 821
SinI GGWCC 2 cut(s) 79, 110
SmaI CCCGGG 1 cut(s) 847
SmiMI CAYNNNNRTG 2 cut(s) 281, 1068
SmlI CTYRAG 3 cut(s) 62, 488, 1028
SmoI CTYRAG 3 cut(s) 62, 488, 1028
Sse9I AATT 4 cut(s) 212, 742, 811, 1043
SsiI CCGC 7 cut(s) 77, 317, 478, 542, 545, 710, 971
SspMI CTAG 3 cut(s) 395, 683, 1252
StyD4I CCNGG 4 cut(s) 344, 452, 844, 845
TaaI ACNGT 4 cut(s) 40, 83, 873, 1172
TaiI ACGT 2 cut(s) 475, 1201
TaqI TCGA 5 cut(s) 220, 608, 1008, 1035, 1219
TaqII GACCGA 1 cut(s) 407
TasI AATT 4 cut(s) 212, 742, 811, 1043
TatI WGTACW 1 cut(s) 484
TauI GCSGC 2 cut(s) 545, 548
TfiI GAWTC 3 cut(s) 504, 677, 865
Tru1I TTAA 3 cut(s) 176, 330, 789
Tru9I TTAA 3 cut(s) 176, 330, 789
TscAI CASTG 2 cut(s) 51, 375
TseFI GTSAC 2 cut(s) 496, 880
TseI GCWGC 3 cut(s) 925, 943, 1235
Tsp45I GTSAC 2 cut(s) 496, 880
TspDTI ATGAA 3 cut(s) 116, 155, 1056
TspGWI ACGGA 1 cut(s) 1108
TspMI CCCGGG 1 cut(s) 845
TspRI CASTG 2 cut(s) 51, 375
Van91I CCANNNNNTGG 1 cut(s) 190
VpaK11BI GGWCC 2 cut(s) 79, 110
XagI CCTNNNNNAGG 1 cut(s) 1028
XcmI CCANNNNNNNNNTGG 3 cut(s) 43, 283, 1067
XmaI CCCGGG 1 cut(s) 845
XmiI GTMKAC 1 cut(s) 1008
XspI CTAG 3 cut(s) 395, 683, 1252
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.