Rmu_sc0002299.1_g000004

Protein timeless homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002299.1
OG0015696
Physical Location & Seq
Reverse (-)
20611 .. 21416
806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002299.1_g000004.1.cds

Sequence Viewer

Length: 456 bp
atggatccggatctgcgcaagctttttatcggcgggatatgcgaagactcagatggattcggatctgagcaagctttgtatcggcaggaattcatgggagacgtcggaggacaatctgaaggagtacttcggcaactactgcaacgttttgcagacggtggttatgagagacaggatcactcaaaggcccagaggtatggcttatcggtgatctgtaacggcctcggaatcgccctagacgacgacggcggaaaccggatcggctacagaaagcacaagtactgcttagataatttgaaggatttgctgaggttcttgaggcgcgatgatccgcagaaccgggatgttttcaaggaagtatgcaagtggaatgttgtatctaaggatttgatacctattattgagcactgccaagatgatcgtagcttggttttaaatgcaggcaagttcttgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIMELESS TIMELESS_C

Protein Analysis

151

Amino Acids

17.2

Weight (kDa)

5.61

Isoelectric Point (pI)

38.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 105
Acc16I TGCGCA 1 cut(s) 17
AccII CGCG 1 cut(s) 324
AccIII TCCGGA 1 cut(s) 7
AciI CCGC 3 cut(s) 33, 249, 332
AclI AACGTT 1 cut(s) 145
AclWI GGATC 6 cut(s) 12, 18, 70, 183, 266, 323
AcsI RAATTY 1 cut(s) 89
AcuI CTGAAG 1 cut(s) 138
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 2 cut(s) 126, 281
AgsI TTSAA 2 cut(s) 298, 352
AluBI AGCT 3 cut(s) 22, 74, 426
AluI AGCT 3 cut(s) 22, 74, 426
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 2 cut(s) 93, 163
AlwI GGATC 6 cut(s) 12, 18, 70, 183, 266, 323
Aor13HI TCCGGA 1 cut(s) 7
AoxI GGCC 2 cut(s) 186, 220
ApoI RAATTY 1 cut(s) 89
AspLEI GCGC 2 cut(s) 18, 324
AspS9I GGNCC 1 cut(s) 187
AsuC2I CCSGG 1 cut(s) 341
AsuHPI GGTGA 1 cut(s) 220
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 408
BbvCI CCTCAGC 1 cut(s) 308
BccI CCATC 1 cut(s) 47
BceAI ACGGC 2 cut(s) 235, 262
BcnI CCSGG 1 cut(s) 341
BcoDI GTCTC 2 cut(s) 93, 163
BfaI CTAG 1 cut(s) 236
BfmI CTRYAG 1 cut(s) 265
BmcAI AGTACT 2 cut(s) 126, 281
Bme1390I CCNGG 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 187
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 341
BpiI GAAGAC 1 cut(s) 51
Bpu10I CCTNAGC 1 cut(s) 308
BpuEI CTTGAG 1 cut(s) 337
BpuMI CCSGG 1 cut(s) 341
BsaBI GATNNNNATC 2 cut(s) 9, 61
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 223
BsaWI WCCGGW 2 cut(s) 7, 255
Bse8I GATNNNNATC 2 cut(s) 9, 61
BseAI TCCGGA 1 cut(s) 7
BseDI CCNNGG 1 cut(s) 223
BseGI GGATG 1 cut(s) 349
BseJI GATNNNNATC 2 cut(s) 9, 61
BseMII CTCAG 3 cut(s) 57, 63, 299
Bsh1236I CGCG 1 cut(s) 324
BshFI GGCC 2 cut(s) 188, 222
BsiHKAI GWGCWC 1 cut(s) 408
BsiSI CCGG 3 cut(s) 8, 256, 340
BsmAI GTCTC 2 cut(s) 93, 163
BsmBI CGTCTC 1 cut(s) 93
BsnI GGCC 2 cut(s) 188, 222
Bsp1286I GDGCHC 1 cut(s) 408
Bsp13I TCCGGA 1 cut(s) 7
Bsp143I GATC 8 cut(s) 4, 10, 62, 175, 210, 258, 328, 418
BspACI CCGC 3 cut(s) 33, 249, 332
BspANI GGCC 2 cut(s) 188, 222
BspCNI CTCAG 3 cut(s) 58, 62, 300
BspEI TCCGGA 1 cut(s) 7
BspFNI CGCG 1 cut(s) 324
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 6 cut(s) 12, 18, 70, 183, 266, 323
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 8 cut(s) 4, 10, 62, 175, 210, 258, 328, 418
BssNI GRCGYC 1 cut(s) 102
Bst4CI ACNGT 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 102
BstAPI GCANNNNNTGC 1 cut(s) 139
BstC8I GCNNGC 3 cut(s) 20, 72, 442
BstDEI CTNAG 5 cut(s) 49, 66, 286, 308, 381
BstF5I GGATG 1 cut(s) 349
BstFNI CGCG 1 cut(s) 324
BstHHI GCGC 2 cut(s) 18, 324
BstKTI GATC 8 cut(s) 7, 13, 65, 178, 213, 261, 331, 421
BstMAI GTCTC 2 cut(s) 93, 163
BstMBI GATC 8 cut(s) 4, 10, 62, 175, 210, 258, 328, 418
BstMWI GCNNNNNNNGC 2 cut(s) 39, 139
BstSCI CCNGG 1 cut(s) 339
BstSFI CTRYAG 1 cut(s) 265
BstUI CGCG 1 cut(s) 324
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 3 cut(s) 4, 10, 62
BstXI CCANNNNNNTGG 1 cut(s) 197
BstYI RGATCY 3 cut(s) 4, 10, 62
BsuRI GGCC 2 cut(s) 188, 222
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 1 cut(s) 349
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 1 cut(s) 406
Cac8I GCNNGC 3 cut(s) 20, 72, 442
CfoI GCGC 2 cut(s) 18, 324
Cfr13I GGNCC 1 cut(s) 187
Csp6I GTAC 2 cut(s) 125, 280
CviAII CATG 1 cut(s) 94
CviJI RGCY 7 cut(s) 22, 74, 188, 201, 222, 264, 426
CviKI_1 RGCY 7 cut(s) 22, 74, 188, 201, 222, 264, 426
CviQI GTAC 2 cut(s) 125, 280
DdeI CTNAG 5 cut(s) 49, 66, 286, 308, 381
DpnI GATC 8 cut(s) 6, 12, 64, 177, 212, 260, 330, 420
DpnII GATC 8 cut(s) 4, 10, 62, 175, 210, 258, 328, 418
DraI TTTAAA 1 cut(s) 435
EciI GGCGGA 1 cut(s) 264
Eco57I CTGAAG 1 cut(s) 138
EcoRI GAATTC 1 cut(s) 89
Esp3I CGTCTC 1 cut(s) 93
FaeI CATG 1 cut(s) 97
FaiI YATR 5 cut(s) 40, 95, 165, 198, 361
FalI AAGNNNNNCTT 4 cut(s) 111, 143, 269, 301
FatI CATG 1 cut(s) 93
FauI CCCGC 1 cut(s) 26
FokI GGATG 1 cut(s) 356
FspBI CTAG 1 cut(s) 236
FspI TGCGCA 1 cut(s) 17
GlaI GCGC 2 cut(s) 17, 323
HaeIII GGCC 2 cut(s) 188, 222
HapII CCGG 3 cut(s) 8, 256, 340
HhaI GCGC 2 cut(s) 18, 324
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 1 cut(s) 97
Hin6I GCGC 2 cut(s) 16, 322
HinP1I GCGC 2 cut(s) 16, 322
HindIII AAGCTT 2 cut(s) 20, 72
HinfI GANTC 3 cut(s) 47, 57, 228
HpaII CCGG 3 cut(s) 8, 256, 340
HphI GGTGA 1 cut(s) 220
Hpy188I TCNGA 6 cut(s) 52, 62, 67, 107, 118, 227
Hpy188III TCNNGA 2 cut(s) 8, 316
Hpy99I CGWCG 3 cut(s) 107, 245, 248
HpyAV CCTTC 2 cut(s) 113, 292
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4IV ACGT 2 cut(s) 102, 145
HpyCH4V TGCA 4 cut(s) 142, 152, 363, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 139
HpyF3I CTNAG 5 cut(s) 49, 66, 286, 308, 381
HpySE526I ACGT 2 cut(s) 102, 145
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 1 cut(s) 97
HspAI GCGC 2 cut(s) 16, 322
Kpn2I TCCGGA 1 cut(s) 7
Kzo9I GATC 8 cut(s) 4, 10, 62, 175, 210, 258, 328, 418
LpnPI CCDG 7 cut(s) 21, 71, 158, 203, 269, 353, 426
MaeI CTAG 1 cut(s) 236
MaeII ACGT 2 cut(s) 102, 145
MaeIII GTNAC 1 cut(s) 215
MalI GATC 8 cut(s) 6, 12, 64, 177, 212, 260, 330, 420
MboI GATC 8 cut(s) 4, 10, 62, 175, 210, 258, 328, 418
MboII GAAGA 1 cut(s) 56
MflI RGATCY 3 cut(s) 4, 10, 62
MhlI GDGCHC 1 cut(s) 408
MluCI AATT 2 cut(s) 89, 292
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 5 cut(s) 101, 186, 233, 303, 312
MroI TCCGGA 1 cut(s) 7
MseI TTAA 1 cut(s) 434
MspI CCGG 3 cut(s) 8, 256, 340
MspR9I CCNGG 1 cut(s) 341
MvnI CGCG 1 cut(s) 324
MwoI GCNNNNNNNGC 2 cut(s) 39, 139
NciI CCSGG 1 cut(s) 341
NdeII GATC 8 cut(s) 4, 10, 62, 175, 210, 258, 328, 418
NlaIII CATG 1 cut(s) 97
NlaIV GGNNCC 1 cut(s) 6
NsbI TGCGCA 1 cut(s) 17
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 2 cut(s) 57, 228
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
Psp1406I AACGTT 1 cut(s) 145
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 187
PsuI RGATCY 3 cut(s) 4, 10, 62
RsaI GTAC 2 cut(s) 126, 281
RsaNI GTAC 2 cut(s) 125, 280
SaqAI TTAA 1 cut(s) 434
Sau3AI GATC 8 cut(s) 4, 10, 62, 175, 210, 258, 328, 418
Sau96I GGNCC 1 cut(s) 187
ScaI AGTACT 2 cut(s) 126, 281
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 1 cut(s) 341
SduI GDGCHC 1 cut(s) 408
SetI ASST 8 cut(s) 24, 76, 105, 148, 197, 314, 397, 428
SfcI CTRYAG 1 cut(s) 265
SmlI CTYRAG 1 cut(s) 316
SmoI CTYRAG 1 cut(s) 316
Sse9I AATT 2 cut(s) 89, 292
SsiI CCGC 3 cut(s) 33, 249, 332
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 1 cut(s) 339
TaaI ACNGT 1 cut(s) 158
TaiI ACGT 2 cut(s) 105, 148
TasI AATT 2 cut(s) 89, 292
TatI WGTACW 2 cut(s) 124, 279
TfiI GAWTC 2 cut(s) 57, 228
Tru1I TTAA 1 cut(s) 434
Tru9I TTAA 1 cut(s) 434
TscAI CASTG 1 cut(s) 413
TspDTI ATGAA 1 cut(s) 82
TspRI CASTG 1 cut(s) 413
XapI RAATTY 1 cut(s) 89
XspI CTAG 1 cut(s) 236
ZraI GACGTC 1 cut(s) 103
ZrmI AGTACT 2 cut(s) 126, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.