Rh2DG235300

Protein timeless homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0015696
Physical Location & Seq
Reverse (-)
23412751 .. 23415743
2993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG235300.1

Sequence Viewer

Length: 801 bp
ATGGATCCGGATCTGCGCAAGCTTTTTATCGGCGGGATATGCGAAGACTCAGATGGATTCGGATCTGAGCAAGCTTTGTATCGGCAGGAATTCATGGGAGACGTCGGAGGACAATCTGAAGGAGTACTTCGGCAACTACCGCAACGTTTTGCAGACGGTGGTTATGAGAGACAGGATCACTCAAAGGCCCAGAGGTATGGCTTATCGGTGATCTGTAACGGCCTCGGAATCGCCCTAGACGACGACGGCGGAAACCGGATCGGCTACAGAAAGCACAAGTACTGCTTAGATAATTTGAAGGATTTGCTGAGGTTCTTGAGGCGCGATGATCCGCAGAACCGGGATGTTTTCAAGGAAGTATGCAAGTGGAATGTTGTATCTAAGGATTTGATACCTATTATTGAGCACTGCCAAGATGATCGTAGCTTGGTTTTAAATGCAGTGAAGGTTCTGGTGTTCTTAACTATGCCAGTTGAGCCTACAGCAAATGACATTTTACAACAAATTGAATTTCTATGGGAGTTGAAGTCTTCAATTATCGATTTCAGGAAAATGTTGGGGAAATTTGGGAAGATCAATAAGCCTGTACGGTTTCCTGAAATTCTGGACTTGGCGCCTTATATGAGTGGTTCAAGTGATAAATCACCCATTTACAAGCTTTATGGGGTGGTTGTTCACTTGGATGTCATGAATGCTGCATTTTCTAGTCATTATGTCTGCTACGTTAAGAACTTGCAGAACAAATGGTTCAAGATTGATGACAGCACGGGTTGGAGAGGTGTGGAAAGAGTTGCAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIMELESS TIMELESS_C

Protein Analysis

266

Amino Acids

30.35

Weight (kDa)

6.53

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIMELESS PF04821 89 - 187 8.2e-24 Timeless protein
UCH PF00443 190 - 256 5.6e-12 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 105
Acc16I TGCGCA 1 cut(s) 17
Acc36I ACCTGC 1 cut(s) 785
AccB1I GGYRCC 1 cut(s) 613
AccII CGCG 1 cut(s) 324
AccIII TCCGGA 1 cut(s) 7
AciI CCGC 4 cut(s) 33, 140, 249, 332
AclI AACGTT 1 cut(s) 145
AclWI GGATC 6 cut(s) 12, 18, 70, 183, 266, 323
AcsI RAATTY 4 cut(s) 89, 509, 563, 600
AcuI CTGAAG 1 cut(s) 138
AcyI GRCGYC 2 cut(s) 102, 614
AfaI GTAC 3 cut(s) 126, 281, 588
AgsI TTSAA 7 cut(s) 298, 352, 509, 526, 534, 633, 751
AluBI AGCT 4 cut(s) 22, 74, 426, 658
AluI AGCT 4 cut(s) 22, 74, 426, 658
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 2 cut(s) 93, 163
AlwI GGATC 6 cut(s) 12, 18, 70, 183, 266, 323
Aor13HI TCCGGA 1 cut(s) 7
AoxI GGCC 2 cut(s) 186, 220
ApeKI GCWGC 1 cut(s) 695
ApoI RAATTY 4 cut(s) 89, 509, 563, 600
AspLEI GCGC 3 cut(s) 18, 324, 616
AspS9I GGNCC 1 cut(s) 187
AsuC2I CCSGG 1 cut(s) 341
AsuHPI GGTGA 2 cut(s) 220, 636
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 613
BbsI GAAGAC 2 cut(s) 51, 522
Bbv12I GWGCWC 1 cut(s) 408
BbvCI CCTCAGC 1 cut(s) 308
BbvI GCAGC 1 cut(s) 682
BccI CCATC 1 cut(s) 47
BceAI ACGGC 2 cut(s) 235, 262
BcnI CCSGG 1 cut(s) 341
BcoDI GTCTC 2 cut(s) 93, 163
BfaI CTAG 2 cut(s) 236, 705
BfmI CTRYAG 2 cut(s) 265, 480
BfoI RGCGCY 1 cut(s) 617
BfuAI ACCTGC 1 cut(s) 785
BisI GCNGC 1 cut(s) 696
BlsI GCNGC 1 cut(s) 697
BmcAI AGTACT 2 cut(s) 126, 281
Bme1390I CCNGG 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 187
BmiI GGNNCC 2 cut(s) 6, 615
BmrFI CCNGG 1 cut(s) 341
BpiI GAAGAC 2 cut(s) 51, 522
Bpu10I CCTNAGC 1 cut(s) 308
BpuEI CTTGAG 1 cut(s) 337
BpuMI CCSGG 1 cut(s) 341
Bsa29I ATCGAT 1 cut(s) 540
BsaBI GATNNNNATC 2 cut(s) 9, 61
BsaHI GRCGYC 2 cut(s) 102, 614
BsaJI CCNNGG 1 cut(s) 223
BsaWI WCCGGW 2 cut(s) 7, 255
BsaXI ACNNNNNCTCC 2 cut(s) 512, 542
Bse1I ACTGG 1 cut(s) 470
Bse8I GATNNNNATC 2 cut(s) 9, 61
BseAI TCCGGA 1 cut(s) 7
BseCI ATCGAT 1 cut(s) 540
BseDI CCNNGG 1 cut(s) 223
BseGI GGATG 2 cut(s) 349, 688
BseJI GATNNNNATC 2 cut(s) 9, 61
BseMII CTCAG 3 cut(s) 57, 63, 299
BseNI ACTGG 1 cut(s) 470
BseXI GCAGC 1 cut(s) 682
Bsh1236I CGCG 1 cut(s) 324
BshFI GGCC 2 cut(s) 188, 222
BshNI GGYRCC 1 cut(s) 613
BshVI ATCGAT 1 cut(s) 540
BsiHKAI GWGCWC 1 cut(s) 408
BsiSI CCGG 3 cut(s) 8, 256, 340
BsmAI GTCTC 2 cut(s) 93, 163
BsmBI CGTCTC 1 cut(s) 93
BsmI GAATGC 1 cut(s) 697
BsnI GGCC 2 cut(s) 188, 222
Bsp1286I GDGCHC 1 cut(s) 408
Bsp13I TCCGGA 1 cut(s) 7
Bsp143I GATC 9 cut(s) 4, 10, 62, 175, 210, 258, 328, 418, 573
BspACI CCGC 4 cut(s) 33, 140, 249, 332
BspANI GGCC 2 cut(s) 188, 222
BspCNI CTCAG 3 cut(s) 58, 62, 300
BspDI ATCGAT 1 cut(s) 540
BspEI TCCGGA 1 cut(s) 7
BspFNI CGCG 1 cut(s) 324
BspHI TCATGA 1 cut(s) 687
BspLI GGNNCC 2 cut(s) 6, 615
BspMI ACCTGC 1 cut(s) 785
BspPI GGATC 6 cut(s) 12, 18, 70, 183, 266, 323
BspT107I GGYRCC 1 cut(s) 613
BsrI ACTGG 1 cut(s) 470
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 9 cut(s) 4, 10, 62, 175, 210, 258, 328, 418, 573
BssNI GRCGYC 2 cut(s) 102, 614
Bst4CI ACNGT 2 cut(s) 158, 591
BstACI GRCGYC 2 cut(s) 102, 614
BstC8I GCNNGC 2 cut(s) 20, 72
BstDEI CTNAG 5 cut(s) 49, 66, 286, 308, 381
BstF5I GGATG 2 cut(s) 349, 688
BstFNI CGCG 1 cut(s) 324
BstH2I RGCGCY 1 cut(s) 617
BstHHI GCGC 3 cut(s) 18, 324, 616
BstKTI GATC 9 cut(s) 7, 13, 65, 178, 213, 261, 331, 421, 576
BstMAI GTCTC 2 cut(s) 93, 163
BstMBI GATC 9 cut(s) 4, 10, 62, 175, 210, 258, 328, 418, 573
BstMWI GCNNNNNNNGC 3 cut(s) 39, 139, 475
BstSCI CCNGG 1 cut(s) 339
BstSFI CTRYAG 2 cut(s) 265, 480
BstUI CGCG 1 cut(s) 324
BstV1I GCAGC 1 cut(s) 682
BstV2I GAAGAC 2 cut(s) 51, 522
BstX2I RGATCY 3 cut(s) 4, 10, 62
BstXI CCANNNNNNTGG 1 cut(s) 197
BstYI RGATCY 3 cut(s) 4, 10, 62
Bsu15I ATCGAT 1 cut(s) 540
BsuRI GGCC 2 cut(s) 188, 222
BsuTUI ATCGAT 1 cut(s) 540
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 2 cut(s) 349, 688
BtsI GCAGTG 2 cut(s) 406, 447
BtsIMutI CAGTG 2 cut(s) 406, 447
BveI ACCTGC 1 cut(s) 785
Cac8I GCNNGC 2 cut(s) 20, 72
CciI TCATGA 1 cut(s) 687
CfoI GCGC 3 cut(s) 18, 324, 616
Cfr13I GGNCC 1 cut(s) 187
ClaI ATCGAT 1 cut(s) 540
Csp6I GTAC 3 cut(s) 125, 280, 587
CviAII CATG 2 cut(s) 94, 688
CviQI GTAC 3 cut(s) 125, 280, 587
DdeI CTNAG 5 cut(s) 49, 66, 286, 308, 381
DinI GGCGCC 1 cut(s) 615
DpnI GATC 9 cut(s) 6, 12, 64, 177, 212, 260, 330, 420, 575
DpnII GATC 9 cut(s) 4, 10, 62, 175, 210, 258, 328, 418, 573
DraI TTTAAA 1 cut(s) 435
EciI GGCGGA 1 cut(s) 264
Eco57I CTGAAG 1 cut(s) 138
EcoRI GAATTC 1 cut(s) 89
EgeI GGCGCC 1 cut(s) 615
EheI GGCGCC 1 cut(s) 615
Esp3I CGTCTC 1 cut(s) 93
FaeI CATG 2 cut(s) 97, 691
FalI AAGNNNNNCTT 4 cut(s) 111, 143, 269, 301
FatI CATG 2 cut(s) 93, 687
FauI CCCGC 1 cut(s) 26
Fnu4HI GCNGC 1 cut(s) 696
FokI GGATG 2 cut(s) 356, 695
Fsp4HI GCNGC 1 cut(s) 696
FspBI CTAG 2 cut(s) 236, 705
FspI TGCGCA 1 cut(s) 17
GlaI GCGC 3 cut(s) 17, 323, 615
GluI GCNGC 1 cut(s) 696
HaeII RGCGCY 1 cut(s) 617
HaeIII GGCC 2 cut(s) 188, 222
HapII CCGG 3 cut(s) 8, 256, 340
HhaI GCGC 3 cut(s) 18, 324, 616
Hin1I GRCGYC 2 cut(s) 102, 614
Hin1II CATG 2 cut(s) 97, 691
Hin6I GCGC 3 cut(s) 16, 322, 614
HinP1I GCGC 3 cut(s) 16, 322, 614
HindIII AAGCTT 3 cut(s) 20, 72, 656
HinfI GANTC 3 cut(s) 47, 57, 228
HpaII CCGG 3 cut(s) 8, 256, 340
HphI GGTGA 2 cut(s) 220, 636
Hpy166II GTNNAC 1 cut(s) 676
Hpy188I TCNGA 6 cut(s) 52, 62, 67, 107, 118, 227
Hpy188III TCNNGA 7 cut(s) 8, 316, 547, 596, 605, 688, 751
Hpy8I GTNNAC 1 cut(s) 676
Hpy99I CGWCG 3 cut(s) 107, 245, 248
HpyAV CCTTC 3 cut(s) 113, 292, 439
HpyCH4III ACNGT 2 cut(s) 158, 591
HpyCH4IV ACGT 3 cut(s) 102, 145, 723
HpyCH4V TGCA 6 cut(s) 152, 363, 440, 698, 736, 794
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 139, 475
HpyF3I CTNAG 5 cut(s) 49, 66, 286, 308, 381
HpySE526I ACGT 3 cut(s) 102, 145, 723
Hsp92I GRCGYC 2 cut(s) 102, 614
Hsp92II CATG 2 cut(s) 97, 691
HspAI GCGC 3 cut(s) 16, 322, 614
KasI GGCGCC 1 cut(s) 613
Kpn2I TCCGGA 1 cut(s) 7
Kzo9I GATC 9 cut(s) 4, 10, 62, 175, 210, 258, 328, 418, 573
Lsp1109I GCAGC 1 cut(s) 682
MaeI CTAG 2 cut(s) 236, 705
MaeII ACGT 3 cut(s) 102, 145, 723
MaeIII GTNAC 1 cut(s) 215
MalI GATC 9 cut(s) 6, 12, 64, 177, 212, 260, 330, 420, 575
MboI GATC 9 cut(s) 4, 10, 62, 175, 210, 258, 328, 418, 573
MboII GAAGA 3 cut(s) 56, 522, 583
MflI RGATCY 3 cut(s) 4, 10, 62
MhlI GDGCHC 1 cut(s) 408
MluCI AATT 7 cut(s) 89, 292, 504, 509, 534, 563, 600
Mly113I GGCGCC 1 cut(s) 614
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 2 cut(s) 85, 752
MnlI CCTC 6 cut(s) 101, 186, 233, 303, 312, 770
MroI TCCGGA 1 cut(s) 7
MseI TTAA 3 cut(s) 434, 461, 726
MslI CAYNNNNRTG 1 cut(s) 681
MspI CCGG 3 cut(s) 8, 256, 340
MspR9I CCNGG 1 cut(s) 341
Mva1269I GAATGC 1 cut(s) 697
MvnI CGCG 1 cut(s) 324
MwoI GCNNNNNNNGC 3 cut(s) 39, 139, 475
NarI GGCGCC 1 cut(s) 614
NciI CCSGG 1 cut(s) 341
NdeII GATC 9 cut(s) 4, 10, 62, 175, 210, 258, 328, 418, 573
NlaIII CATG 2 cut(s) 97, 691
NlaIV GGNNCC 2 cut(s) 6, 615
NsbI TGCGCA 1 cut(s) 17
PagI TCATGA 1 cut(s) 687
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PctI GAATGC 1 cut(s) 697
PfeI GAWTC 2 cut(s) 57, 228
PkrI GCNGC 1 cut(s) 697
PleI GAGTC 1 cut(s) 41
PluTI GGCGCC 1 cut(s) 617
PpsI GAGTC 1 cut(s) 41
Psp1406I AACGTT 1 cut(s) 145
PspN4I GGNNCC 2 cut(s) 6, 615
PspPI GGNCC 1 cut(s) 187
PsuI RGATCY 3 cut(s) 4, 10, 62
RsaI GTAC 3 cut(s) 126, 281, 588
RsaNI GTAC 3 cut(s) 125, 280, 587
RseI CAYNNNNRTG 1 cut(s) 681
SaqAI TTAA 3 cut(s) 434, 461, 726
SatI GCNGC 1 cut(s) 696
Sau3AI GATC 9 cut(s) 4, 10, 62, 175, 210, 258, 328, 418, 573
Sau96I GGNCC 1 cut(s) 187
ScaI AGTACT 2 cut(s) 126, 281
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 1 cut(s) 341
SduI GDGCHC 1 cut(s) 408
SfcI CTRYAG 2 cut(s) 265, 480
SfoI GGCGCC 1 cut(s) 615
SmiMI CAYNNNNRTG 1 cut(s) 681
SmlI CTYRAG 1 cut(s) 316
SmoI CTYRAG 1 cut(s) 316
Sse9I AATT 7 cut(s) 89, 292, 504, 509, 534, 563, 600
SsiI CCGC 4 cut(s) 33, 140, 249, 332
SspDI GGCGCC 1 cut(s) 613
SspMI CTAG 2 cut(s) 236, 705
StyD4I CCNGG 1 cut(s) 339
TaaI ACNGT 2 cut(s) 158, 591
TaiI ACGT 3 cut(s) 105, 148, 726
TaqI TCGA 1 cut(s) 540
TasI AATT 7 cut(s) 89, 292, 504, 509, 534, 563, 600
TatI WGTACW 2 cut(s) 124, 279
TfiI GAWTC 2 cut(s) 57, 228
Tru1I TTAA 3 cut(s) 434, 461, 726
Tru9I TTAA 3 cut(s) 434, 461, 726
TscAI CASTG 2 cut(s) 413, 447
TseI GCWGC 1 cut(s) 695
TspDTI ATGAA 2 cut(s) 82, 704
TspRI CASTG 2 cut(s) 413, 447
XapI RAATTY 4 cut(s) 89, 509, 563, 600
XspI CTAG 2 cut(s) 236, 705
ZraI GACGTC 1 cut(s) 103
ZrmI AGTACT 2 cut(s) 126, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.