Rmu_sc0002538.1_g000026

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002538.1
OG0002814
Physical Location & Seq
Forward (+)
102104 .. 104566
2463 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 978 bp
atgtctaagctagccatggatgttcctcctaagggtgggtttagctttgatctctgtaagcgaaatgcgatgctggccaaggatggggttcagctacctccttttagaaagaccggaaccaccattgtgggtttgatttatcaggatggcgtggtcctcggagcagatacaagggccactgaaggacccattgtagctgataagaactgcgagaagatccattacttggcgcccaatatttattgctgtggtgcgggaactgctgctgacactgaggcagttacagatatggtaagcgcacagctgcagttgcaccgttaccataccggcagggagtcaagggttgtgactgccctcacctatctcaaaagacatcttttcagttaccaaggtcatgtctcggctgcattggtgctgggtggtgttgatttcaccggccctcatttgcacactatatatcctcatggatctactgacacacttccttatgctactatgggctctggctcactggctgcgatggcaatatttgagtccaagtacaaggaggggttaacaagagatgaaggtgtacaattggtgacagaagctatttgtgcaggaatcttcaatgaccttggaagtggtagcaatgttgacatttgtgttataaccaagggaagcaaagaatatctccgaaaccaccttcagcctaatccccgcacatatgttactcccaaggaatatactttcagcaagaagactgaagtactctccacccttgttactacactgaaggagagagtagaagtggtggaagctggagataaaatggaagagctggtgcaaaaagtgctgccggcacacaaacgagaaagttggcagaactggccatcagcactatacccgcacgttgccaggcctatttccacggatgacctcagaccgagacttgagaggaaggctacttatgatgagactaaaaatgatatcaactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

325

Amino Acids

35.78

Weight (kDa)

7.16

Isoelectric Point (pI)

29.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 37 - 217 9e-50 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 650
AccB1I GGYRCC 1 cut(s) 229
AccB7I CCANNNNNTGG 1 cut(s) 226
AciI CCGC 3 cut(s) 254, 700, 887
AclWI GGATC 2 cut(s) 211, 475
AcoI YGGCCR 2 cut(s) 75, 869
AcuI CTGAAG 4 cut(s) 201, 671, 765, 794
AcyI GRCGYC 1 cut(s) 230
AfaI GTAC 3 cut(s) 542, 573, 750
AfiI CCNNNNNNNGG 4 cut(s) 32, 35, 84, 226
AgsI TTSAA 1 cut(s) 610
AjnI CCWGG 1 cut(s) 896
AleI CACNNNNGTG 1 cut(s) 125
AluBI AGCT 8 cut(s) 10, 45, 94, 197, 304, 590, 800, 820
AluI AGCT 8 cut(s) 10, 45, 94, 197, 304, 590, 800, 820
Alw26I GTCTC 3 cut(s) 403, 922, 950
AlwI GGATC 2 cut(s) 211, 475
AoxI GGCC 5 cut(s) 75, 174, 436, 869, 899
ApeKI GCWGC 5 cut(s) 263, 304, 404, 515, 835
AspLEI GCGC 2 cut(s) 232, 299
AspS9I GGNCC 4 cut(s) 154, 174, 185, 437
AsuHPI GGTGA 3 cut(s) 349, 424, 592
AsuNHI GCTAGC 1 cut(s) 10
AvaII GGWCC 2 cut(s) 154, 185
AxyI CCTNAGG 1 cut(s) 30
BalI TGGCCA 2 cut(s) 77, 871
BanI GGYRCC 1 cut(s) 229
BanII GRGCYC 1 cut(s) 503
BarI GAAGNNNNNNTAC 2 cut(s) 206, 238
BbsI GAAGAC 1 cut(s) 746
BbvI GCAGC 5 cut(s) 250, 291, 391, 502, 822
BccI CCATC 4 cut(s) 77, 140, 514, 880
BciT130I CCWGG 1 cut(s) 898
BcoDI GTCTC 3 cut(s) 403, 922, 950
BfaI CTAG 1 cut(s) 11
BfmI CTRYAG 1 cut(s) 305
BfoI RGCGCY 1 cut(s) 233
BisI GCNGC 5 cut(s) 264, 305, 405, 516, 836
BlsI GCNGC 5 cut(s) 265, 306, 406, 517, 837
BmcAI AGTACT 1 cut(s) 750
Bme1390I CCNGG 1 cut(s) 898
Bme18I GGWCC 2 cut(s) 154, 185
BmgT120I GGNCC 4 cut(s) 154, 174, 185, 437
BmiI GGNNCC 3 cut(s) 118, 187, 231
BmrFI CCNGG 1 cut(s) 898
BmsI GCATC 1 cut(s) 60
BmtI GCTAGC 1 cut(s) 14
BpiI GAAGAC 1 cut(s) 746
BpmI CTGGAG 1 cut(s) 822
BpuEI CTTGAG 1 cut(s) 953
BsaHI GRCGYC 1 cut(s) 230
BsaJI CCNNGG 8 cut(s) 15, 78, 157, 388, 616, 654, 717, 909
BsaWI WCCGGW 1 cut(s) 113
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 35, 84, 226
Bse118I RCCGGY 3 cut(s) 326, 434, 838
Bse1I ACTGG 2 cut(s) 516, 872
Bse21I CCTNAGG 1 cut(s) 30
Bse3DI GCAATG 1 cut(s) 637
BseBI CCWGG 1 cut(s) 898
BseDI CCNNGG 8 cut(s) 15, 78, 157, 388, 616, 654, 717, 909
BseGI GGATG 4 cut(s) 25, 88, 151, 919
BseLI CCNNNNNNNGG 4 cut(s) 32, 35, 84, 226
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 2 cut(s) 264, 934
BseNI ACTGG 2 cut(s) 516, 872
BseXI GCAGC 5 cut(s) 250, 291, 391, 502, 822
BseYI CCCAGC 1 cut(s) 415
BsgI GTGCAG 1 cut(s) 618
BshFI GGCC 5 cut(s) 77, 176, 438, 871, 901
BshNI GGYRCC 1 cut(s) 229
BsiSI CCGG 4 cut(s) 114, 327, 435, 839
BslI CCNNNNNNNGG 4 cut(s) 32, 35, 84, 226
BsmAI GTCTC 3 cut(s) 403, 922, 950
BsnI GGCC 5 cut(s) 77, 176, 438, 871, 901
Bsp1286I GDGCHC 1 cut(s) 503
Bsp1407I TGTACA 1 cut(s) 571
Bsp143I GATC 3 cut(s) 49, 216, 467
Bsp19I CCATGG 1 cut(s) 15
BspACI CCGC 3 cut(s) 254, 700, 887
BspANI GGCC 5 cut(s) 77, 176, 438, 871, 901
BspCNI CTCAG 2 cut(s) 265, 933
BspLI GGNNCC 3 cut(s) 118, 187, 231
BspMAI CTGCAG 1 cut(s) 309
BspOI GCTAGC 1 cut(s) 14
BspPI GGATC 2 cut(s) 211, 475
BspQI GCTCTTC 1 cut(s) 810
BspT107I GGYRCC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 637
BsrFI RCCGGY 3 cut(s) 326, 434, 838
BsrGI TGTACA 1 cut(s) 571
BsrI ACTGG 2 cut(s) 516, 872
BssAI RCCGGY 3 cut(s) 326, 434, 838
BssECI CCNNGG 8 cut(s) 15, 78, 157, 388, 616, 654, 717, 909
BssMI GATC 3 cut(s) 49, 216, 467
BssNI GRCGYC 1 cut(s) 230
BssT1I CCWWGG 6 cut(s) 15, 78, 388, 616, 654, 717
Bst2UI CCWGG 1 cut(s) 898
Bst4CI ACNGT 1 cut(s) 317
Bst6I CTCTTC 1 cut(s) 810
BstACI GRCGYC 1 cut(s) 230
BstAPI GCANNNNNTGC 1 cut(s) 832
BstAUI TGTACA 1 cut(s) 571
BstC8I GCNNGC 3 cut(s) 12, 75, 840
BstDEI CTNAG 4 cut(s) 6, 30, 273, 920
BstDSI CCRYGG 2 cut(s) 15, 909
BstF5I GGATG 4 cut(s) 25, 88, 151, 919
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 2 cut(s) 232, 299
BstKTI GATC 3 cut(s) 52, 219, 470
BstMAI GTCTC 3 cut(s) 403, 922, 950
BstMBI GATC 3 cut(s) 49, 216, 467
BstMWI GCNNNNNNNGC 7 cut(s) 74, 260, 310, 521, 596, 832, 868
BstNI CCWGG 1 cut(s) 898
BstSCI CCNGG 1 cut(s) 896
BstSFI CTRYAG 1 cut(s) 305
BstV1I GCAGC 5 cut(s) 250, 291, 391, 502, 822
BstV2I GAAGAC 1 cut(s) 746
BstX2I RGATCY 2 cut(s) 216, 467
BstXI CCANNNNNNTGG 1 cut(s) 127
BstYI RGATCY 2 cut(s) 216, 467
Bsu36I CCTNAGG 1 cut(s) 30
BsuRI GGCC 5 cut(s) 77, 176, 438, 871, 901
BtgI CCRYGG 2 cut(s) 15, 909
BtgZI GCGATG 2 cut(s) 83, 533
BtsCI GGATG 4 cut(s) 25, 88, 151, 919
BtsIMutI CAGTG 4 cut(s) 177, 270, 509, 770
Cac8I GCNNGC 3 cut(s) 12, 75, 840
CfoI GCGC 2 cut(s) 232, 299
Cfr10I RCCGGY 3 cut(s) 326, 434, 838
Cfr13I GGNCC 4 cut(s) 154, 174, 185, 437
Csp6I GTAC 3 cut(s) 541, 572, 749
CviAII CATG 3 cut(s) 16, 395, 464
CviQI GTAC 3 cut(s) 541, 572, 749
DdeI CTNAG 4 cut(s) 6, 30, 273, 920
DinI GGCGCC 1 cut(s) 231
DpnI GATC 3 cut(s) 51, 218, 469
DpnII GATC 3 cut(s) 49, 216, 467
EaeI YGGCCR 2 cut(s) 75, 869
Eam1104I CTCTTC 1 cut(s) 810
EarI CTCTTC 1 cut(s) 810
Eco130I CCWWGG 6 cut(s) 15, 78, 388, 616, 654, 717
Eco147I AGGCCT 1 cut(s) 901
Eco24I GRGCYC 1 cut(s) 503
Eco32I GATATC 1 cut(s) 970
Eco47I GGWCC 2 cut(s) 154, 185
Eco57I CTGAAG 4 cut(s) 201, 671, 765, 794
Eco81I CCTNAGG 1 cut(s) 30
EcoO109I RGGNCCY 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 896
EcoRV GATATC 1 cut(s) 970
EcoT14I CCWWGG 6 cut(s) 15, 78, 388, 616, 654, 717
EcoT38I GRGCYC 1 cut(s) 503
EgeI GGCGCC 1 cut(s) 231
EheI GGCGCC 1 cut(s) 231
ErhI CCWWGG 6 cut(s) 15, 78, 388, 616, 654, 717
FaeI CATG 3 cut(s) 19, 398, 467
FatI CATG 3 cut(s) 15, 394, 463
FauI CCCGC 3 cut(s) 247, 707, 894
FauNDI CATATG 1 cut(s) 706
Fnu4HI GCNGC 5 cut(s) 264, 305, 405, 516, 836
FokI GGATG 4 cut(s) 32, 95, 158, 926
FriOI GRGCYC 1 cut(s) 503
Fsp4HI GCNGC 5 cut(s) 264, 305, 405, 516, 836
FspBI CTAG 1 cut(s) 11
GlaI GCGC 2 cut(s) 231, 298
GluI GCNGC 5 cut(s) 264, 305, 405, 516, 836
GsaI CCCAGC 1 cut(s) 419
GsuI CTGGAG 1 cut(s) 822
HaeII RGCGCY 1 cut(s) 233
HaeIII GGCC 5 cut(s) 77, 176, 438, 871, 901
HapII CCGG 4 cut(s) 114, 327, 435, 839
HhaI GCGC 2 cut(s) 232, 299
Hin1I GRCGYC 1 cut(s) 230
Hin1II CATG 3 cut(s) 19, 398, 467
Hin6I GCGC 2 cut(s) 230, 297
HinP1I GCGC 2 cut(s) 230, 297
HincII GTYRAC 2 cut(s) 555, 637
HindII GTYRAC 2 cut(s) 555, 637
HinfI GANTC 3 cut(s) 335, 533, 603
HpaI GTTAAC 1 cut(s) 555
HpaII CCGG 4 cut(s) 114, 327, 435, 839
HphI GGTGA 3 cut(s) 349, 424, 592
Hpy166II GTNNAC 3 cut(s) 555, 572, 637
Hpy188I TCNGA 3 cut(s) 161, 677, 923
Hpy188III TCNNGA 1 cut(s) 143
Hpy8I GTNNAC 3 cut(s) 555, 572, 637
HpyAV CCTTC 5 cut(s) 176, 560, 695, 769, 934
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 1 cut(s) 891
HpyCH4V TGCA 6 cut(s) 307, 313, 407, 448, 599, 826
HpyF10VI GCNNNNNNNGC 7 cut(s) 74, 260, 310, 521, 596, 832, 868
HpyF3I CTNAG 4 cut(s) 6, 30, 273, 920
HpySE526I ACGT 1 cut(s) 891
Hsp92I GRCGYC 1 cut(s) 230
Hsp92II CATG 3 cut(s) 19, 398, 467
HspAI GCGC 2 cut(s) 230, 297
KasI GGCGCC 1 cut(s) 229
KroI GCCGGC 1 cut(s) 838
KroNI GCCGGC 1 cut(s) 840
KspAI GTTAAC 1 cut(s) 555
Kzo9I GATC 3 cut(s) 49, 216, 467
LguI GCTCTTC 1 cut(s) 810
LmnI GCTCC 1 cut(s) 161
Lsp1109I GCAGC 5 cut(s) 250, 291, 391, 502, 822
LweI GCATC 1 cut(s) 60
MaeI CTAG 1 cut(s) 11
MaeII ACGT 1 cut(s) 891
MaeIII GTNAC 7 cut(s) 280, 317, 346, 383, 580, 709, 763
MalI GATC 3 cut(s) 51, 218, 469
MboI GATC 3 cut(s) 49, 216, 467
MboII GAAGA 4 cut(s) 226, 598, 751, 827
MfeI CAATTG 1 cut(s) 575
MflI RGATCY 2 cut(s) 216, 467
MhlI GDGCHC 1 cut(s) 503
MlsI TGGCCA 2 cut(s) 77, 871
MluCI AATT 1 cut(s) 575
MluNI TGGCCA 2 cut(s) 77, 871
Mly113I GGCGCC 1 cut(s) 230
MlyI GAGTC 2 cut(s) 344, 542
Mox20I TGGCCA 2 cut(s) 77, 871
MroNI GCCGGC 1 cut(s) 838
MscI TGGCCA 2 cut(s) 77, 871
MseI TTAA 1 cut(s) 554
MslI CAYNNNNRTG 1 cut(s) 125
Msp20I TGGCCA 2 cut(s) 77, 871
MspA1I CMGCKG 1 cut(s) 304
MspI CCGG 4 cut(s) 114, 327, 435, 839
MspR9I CCNGG 1 cut(s) 898
MunI CAATTG 1 cut(s) 575
MvaI CCWGG 1 cut(s) 898
MwoI GCNNNNNNNGC 7 cut(s) 74, 260, 310, 521, 596, 832, 868
NaeI GCCGGC 1 cut(s) 840
NarI GGCGCC 1 cut(s) 230
NcoI CCATGG 1 cut(s) 15
NdeI CATATG 1 cut(s) 706
NdeII GATC 3 cut(s) 49, 216, 467
NgoMIV GCCGGC 1 cut(s) 838
NheI GCTAGC 1 cut(s) 10
NlaIII CATG 3 cut(s) 19, 398, 467
NlaIV GGNNCC 3 cut(s) 118, 187, 231
NmeAIII GCCGAG 1 cut(s) 380
NmuCI GTSAC 2 cut(s) 346, 580
OliI CACNNNNGTG 1 cut(s) 125
PceI AGGCCT 1 cut(s) 901
PciSI GCTCTTC 1 cut(s) 810
PdiI GCCGGC 1 cut(s) 840
PfeI GAWTC 1 cut(s) 603
PflMI CCANNNNNTGG 1 cut(s) 226
PkrI GCNGC 5 cut(s) 265, 306, 406, 517, 837
PleI GAGTC 2 cut(s) 343, 541
PluTI GGCGCC 1 cut(s) 233
PpsI GAGTC 2 cut(s) 343, 541
PpuMI RGGWCCY 1 cut(s) 185
PsiI TTATAA 1 cut(s) 650
Psp5II RGGWCCY 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 896
PspFI CCCAGC 1 cut(s) 415
PspGI CCWGG 1 cut(s) 896
PspN4I GGNNCC 3 cut(s) 118, 187, 231
PspPI GGNCC 4 cut(s) 154, 174, 185, 437
PspPPI RGGWCCY 1 cut(s) 185
PstI CTGCAG 1 cut(s) 309
PsuI RGATCY 2 cut(s) 216, 467
PvuII CAGCTG 1 cut(s) 304
RsaI GTAC 3 cut(s) 542, 573, 750
RsaNI GTAC 3 cut(s) 541, 572, 749
RseI CAYNNNNRTG 1 cut(s) 125
SapI GCTCTTC 1 cut(s) 810
SaqAI TTAA 1 cut(s) 554
SatI GCNGC 5 cut(s) 264, 305, 405, 516, 836
Sau3AI GATC 3 cut(s) 49, 216, 467
Sau96I GGNCC 4 cut(s) 154, 174, 185, 437
ScaI AGTACT 1 cut(s) 750
SchI GAGTC 2 cut(s) 344, 542
ScrFI CCNGG 1 cut(s) 898
SduI GDGCHC 1 cut(s) 503
SfaNI GCATC 1 cut(s) 60
SfcI CTRYAG 1 cut(s) 305
SfoI GGCGCC 1 cut(s) 231
SinI GGWCC 2 cut(s) 154, 185
SmiMI CAYNNNNRTG 1 cut(s) 125
SmlI CTYRAG 1 cut(s) 932
SmoI CTYRAG 1 cut(s) 932
Sse9I AATT 1 cut(s) 575
SseBI AGGCCT 1 cut(s) 901
SsiI CCGC 3 cut(s) 254, 700, 887
SspDI GGCGCC 1 cut(s) 229
SspI AATATT 2 cut(s) 238, 528
SspMI CTAG 1 cut(s) 11
StuI AGGCCT 1 cut(s) 901
StyD4I CCNGG 1 cut(s) 896
StyI CCWWGG 6 cut(s) 15, 78, 388, 616, 654, 717
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 1 cut(s) 894
TaqII GACCGA 1 cut(s) 940
TasI AATT 1 cut(s) 575
TatI WGTACW 3 cut(s) 540, 571, 748
TfiI GAWTC 1 cut(s) 603
Tru1I TTAA 1 cut(s) 554
Tru9I TTAA 1 cut(s) 554
TscAI CASTG 4 cut(s) 184, 277, 516, 777
TseFI GTSAC 2 cut(s) 346, 580
TseI GCWGC 5 cut(s) 263, 304, 404, 515, 835
Tsp45I GTSAC 2 cut(s) 346, 580
TspDTI ATGAA 1 cut(s) 579
TspGWI ACGGA 1 cut(s) 926
TspRI CASTG 4 cut(s) 184, 277, 516, 777
Van91I CCANNNNNTGG 1 cut(s) 226
VpaK11BI GGWCC 2 cut(s) 154, 185
XspI CTAG 1 cut(s) 11
ZrmI AGTACT 1 cut(s) 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.