Rmu_sc0002784.1_g000051

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002784.1
OG0015770
Physical Location & Seq
Forward (+)
217227 .. 218940
1714 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 531 bp
atgtgtctgattgagactttggatgagatggttcagaacgggactctaagtcctgagcttgccattcaagttctggttcagttcgaaaaaggagacccggccataactcctccgtccagccatctctcggcggcgcaccactggcgttcgactcctcttctcagtgccaacatgcctatggtggtggaccatacatgcacacatagtggggagagattcaaaacccaaaagcctgaagcttttccggcgactctggctgctgggttcgtttctgaagtcgtggaacaccgattgatggctagtggagctcaattcattgaattgagtaaagtggtgagctcggataccgaactgaataagaggaattggatgggctctgctcgacgatcacagagcatagtagctgcaagttatgcttgttcaagatctctgtgggtctatgaaggagtcattgatgatcgttatggtgaatgtttcattgctgagaacaaatctcttcaaaaggtattgtctgctctaaatgtgaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0003674 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005672 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0016591 GO:0017025 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022607 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051090 GO:0051091 GO:0051123 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0060255 GO:0061695 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070013 GO:0070897 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090575 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05203 map03022 map05203
Pfam Domains
Protein Families
TFIIA_gamma_C TFIIA_gamma_N

Protein Analysis

176

Amino Acids

19.38

Weight (kDa)

6.06

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIA_gamma_N PF02268 1 - 30 8.9e-08 Transcription initiation factor IIA, gamma subunit, helical domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 131
AcoI YGGCCR 1 cut(s) 99
AcuI CTGAAG 2 cut(s) 255, 294
AdeI CACNNNGTG 1 cut(s) 206
AfiI CCNNNNNNNGG 2 cut(s) 127, 295
AgsI TTSAA 5 cut(s) 68, 220, 320, 423, 500
AhdI GACNNNNNGTC 1 cut(s) 48
AluBI AGCT 5 cut(s) 58, 239, 308, 339, 404
AluI AGCT 5 cut(s) 58, 239, 308, 339, 404
Alw21I GWGCWC 2 cut(s) 310, 341
Alw26I GTCTC 2 cut(s) 8, 87
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 2 cut(s) 257, 404
Asp700I GAANNNNTTC 1 cut(s) 240
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 1 cut(s) 187
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 2 cut(s) 346, 479
AsuII TTCGAA 1 cut(s) 84
AvaII GGWCC 1 cut(s) 187
BanII GRGCYC 3 cut(s) 310, 341, 377
Bbv12I GWGCWC 2 cut(s) 310, 341
BbvI GCAGC 2 cut(s) 244, 391
BccI CCATC 4 cut(s) 22, 129, 289, 364
BciVI GTATCC 1 cut(s) 337
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 2 cut(s) 8, 87
BfaI CTAG 1 cut(s) 300
BfuI GTATCC 1 cut(s) 337
BglII AGATCT 1 cut(s) 425
BisI GCNGC 3 cut(s) 132, 258, 405
BlsI GCNGC 3 cut(s) 133, 259, 406
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 187
BmeRI GACNNNNNGTC 1 cut(s) 48
BmgT120I GGNCC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 98
Bpu10I CCTNAGC 1 cut(s) 54
Bpu14I TTCGAA 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 98
BsaI GGTCTC 1 cut(s) 87
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 295
Bse1I ACTGG 1 cut(s) 146
Bse3DI GCAATG 1 cut(s) 477
BseGI GGATG 2 cut(s) 28, 375
BseLI CCNNNNNNNGG 2 cut(s) 127, 295
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 3 cut(s) 45, 175, 474
BseNI ACTGG 1 cut(s) 146
BseRI GAGGAG 2 cut(s) 99, 144
BseXI GCAGC 2 cut(s) 244, 391
BseYI CCCAGC 1 cut(s) 260
BshFI GGCC 1 cut(s) 101
BsiHKAI GWGCWC 2 cut(s) 310, 341
BsiSI CCGG 2 cut(s) 98, 245
BslFI GGGAC 1 cut(s) 55
BslI CCNNNNNNNGG 2 cut(s) 127, 295
BsmAI GTCTC 2 cut(s) 8, 87
BsmFI GGGAC 1 cut(s) 55
BsnI GGCC 1 cut(s) 101
Bso31I GGTCTC 1 cut(s) 87
Bsp119I TTCGAA 1 cut(s) 84
Bsp1286I GDGCHC 3 cut(s) 310, 341, 377
Bsp143I GATC 3 cut(s) 386, 425, 457
BspACI CCGC 1 cut(s) 131
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 3 cut(s) 46, 174, 475
BspT104I TTCGAA 1 cut(s) 84
BspTNI GGTCTC 1 cut(s) 87
BsrDI GCAATG 1 cut(s) 477
BsrI ACTGG 1 cut(s) 146
BssMI GATC 3 cut(s) 386, 425, 457
Bst6I CTCTTC 2 cut(s) 162, 501
BstAPI GCANNNNNTGC 1 cut(s) 413
BstBI TTCGAA 1 cut(s) 84
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 4 cut(s) 47, 54, 161, 483
BstF5I GGATG 2 cut(s) 28, 375
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 3 cut(s) 389, 428, 460
BstMAI GTCTC 2 cut(s) 8, 87
BstMBI GATC 3 cut(s) 386, 425, 457
BstMWI GCNNNNNNNGC 5 cut(s) 142, 245, 254, 305, 413
BstNSI RCATGY 2 cut(s) 175, 198
BstSCI CCNGG 1 cut(s) 96
BstV1I GCAGC 2 cut(s) 244, 391
BstX2I RGATCY 1 cut(s) 425
BstYI RGATCY 1 cut(s) 425
BsuI GTATCC 1 cut(s) 337
BsuRI GGCC 1 cut(s) 101
BtsCI GGATG 2 cut(s) 28, 375
BtsIMutI CAGTG 2 cut(s) 139, 169
Cac8I GCNNGC 1 cut(s) 60
CfoI GCGC 1 cut(s) 136
Cfr13I GGNCC 1 cut(s) 187
CviAII CATG 2 cut(s) 172, 195
DdeI CTNAG 4 cut(s) 47, 54, 161, 483
DpnI GATC 3 cut(s) 388, 427, 459
DpnII GATC 3 cut(s) 386, 425, 457
DraIII CACNNNGTG 1 cut(s) 206
DriI GACNNNNNGTC 1 cut(s) 48
EaeI YGGCCR 1 cut(s) 99
Eam1104I CTCTTC 2 cut(s) 162, 501
Eam1105I GACNNNNNGTC 1 cut(s) 48
EarI CTCTTC 2 cut(s) 162, 501
Ecl136II GAGCTC 2 cut(s) 308, 339
Eco24I GRGCYC 3 cut(s) 310, 341, 377
Eco31I GGTCTC 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 187
Eco53kI GAGCTC 2 cut(s) 308, 339
Eco57I CTGAAG 2 cut(s) 255, 294
EcoICRI GAGCTC 2 cut(s) 308, 339
EcoT38I GRGCYC 3 cut(s) 310, 341, 377
FaeI CATG 2 cut(s) 175, 198
FalI AAGNNNNNCTT 2 cut(s) 400, 432
FaqI GGGAC 1 cut(s) 55
FatI CATG 2 cut(s) 171, 194
Fnu4HI GCNGC 3 cut(s) 132, 258, 405
FokI GGATG 2 cut(s) 35, 382
FriOI GRGCYC 3 cut(s) 310, 341, 377
Fsp4HI GCNGC 3 cut(s) 132, 258, 405
FspBI CTAG 1 cut(s) 300
GlaI GCGC 1 cut(s) 135
GluI GCNGC 3 cut(s) 132, 258, 405
GsaI CCCAGC 1 cut(s) 264
HaeIII GGCC 1 cut(s) 101
HapII CCGG 2 cut(s) 98, 245
HhaI GCGC 1 cut(s) 136
Hin1II CATG 2 cut(s) 175, 198
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HindIII AAGCTT 1 cut(s) 237
HinfI GANTC 5 cut(s) 43, 151, 216, 250, 447
HpaII CCGG 2 cut(s) 98, 245
HphI GGTGA 2 cut(s) 346, 479
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 4 cut(s) 9, 36, 274, 343
Hpy188III TCNNGA 2 cut(s) 53, 423
Hpy8I GTNNAC 1 cut(s) 187
Hpy99I CGWCG 1 cut(s) 387
HpyAV CCTTC 1 cut(s) 437
HpyCH4V TGCA 2 cut(s) 198, 407
HpyF10VI GCNNNNNNNGC 5 cut(s) 142, 245, 254, 305, 413
HpyF3I CTNAG 4 cut(s) 47, 54, 161, 483
Hsp92II CATG 2 cut(s) 175, 198
HspAI GCGC 1 cut(s) 134
Kzo9I GATC 3 cut(s) 386, 425, 457
LmnI GCTCC 1 cut(s) 305
LpnPI CCDG 9 cut(s) 59, 66, 111, 127, 130, 239, 246, 246, 258
Lsp1109I GCAGC 2 cut(s) 244, 391
MaeI CTAG 1 cut(s) 300
MalI GATC 3 cut(s) 388, 427, 459
MboI GATC 3 cut(s) 386, 425, 457
MboII GAAGA 2 cut(s) 149, 488
MflI RGATCY 1 cut(s) 425
MhlI GDGCHC 3 cut(s) 310, 341, 377
MluCI AATT 3 cut(s) 311, 320, 364
MlyI GAGTC 4 cut(s) 37, 145, 244, 456
MnlI CCTC 3 cut(s) 120, 165, 354
MroXI GAANNNNTTC 1 cut(s) 240
MslI CAYNNNNRTG 1 cut(s) 176
MspI CCGG 2 cut(s) 98, 245
MspR9I CCNGG 1 cut(s) 98
MwoI GCNNNNNNNGC 5 cut(s) 142, 245, 254, 305, 413
NciI CCSGG 1 cut(s) 98
NdeII GATC 3 cut(s) 386, 425, 457
NlaIII CATG 2 cut(s) 175, 198
NmeAIII GCCGAG 1 cut(s) 107
NspI RCATGY 2 cut(s) 175, 198
NspV TTCGAA 1 cut(s) 84
PdmI GAANNNNTTC 1 cut(s) 240
PfeI GAWTC 1 cut(s) 216
PkrI GCNGC 3 cut(s) 133, 259, 406
PleI GAGTC 4 cut(s) 37, 145, 244, 455
PpsI GAGTC 4 cut(s) 37, 145, 244, 455
Psp124BI GAGCTC 2 cut(s) 310, 341
PspFI CCCAGC 1 cut(s) 260
PspPI GGNCC 1 cut(s) 187
PsuI RGATCY 1 cut(s) 425
RseI CAYNNNNRTG 1 cut(s) 176
SacI GAGCTC 2 cut(s) 310, 341
SatI GCNGC 3 cut(s) 132, 258, 405
Sau3AI GATC 3 cut(s) 386, 425, 457
Sau96I GGNCC 1 cut(s) 187
SchI GAGTC 4 cut(s) 37, 145, 244, 456
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 3 cut(s) 310, 341, 377
SetI ASST 6 cut(s) 60, 241, 310, 341, 406, 507
SfuI TTCGAA 1 cut(s) 84
SinI GGWCC 1 cut(s) 187
SmiMI CAYNNNNRTG 1 cut(s) 176
Sse9I AATT 3 cut(s) 311, 320, 364
SsiI CCGC 1 cut(s) 131
SspMI CTAG 1 cut(s) 300
SstI GAGCTC 2 cut(s) 310, 341
StyD4I CCNGG 1 cut(s) 96
TaqI TCGA 3 cut(s) 84, 149, 382
TasI AATT 3 cut(s) 311, 320, 364
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 1 cut(s) 216
TscAI CASTG 2 cut(s) 146, 169
TseI GCWGC 2 cut(s) 257, 404
TspDTI ATGAA 3 cut(s) 304, 456, 466
TspGWI ACGGA 1 cut(s) 102
TspRI CASTG 2 cut(s) 146, 169
VpaK11BI GGWCC 1 cut(s) 187
XceI RCATGY 2 cut(s) 175, 198
XcmI CCANNNNNNNNNTGG 2 cut(s) 70, 175
XmnI GAANNNNTTC 1 cut(s) 240
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.