Rroxscaffold_1G00034790

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015770
Physical Location & Seq
Reverse (-)
50533009 .. 50536275
3267 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00034790.1

Sequence Viewer

Length: 534 bp
ATGGTTCGACCGGGACTCTCGGTCCCGAGCTTGCCATTCGTGTTCGGTTCGGTTCAAAAAAGGAGACCCGGCCATAACTCCTCGGTCCGAGCATCTCTCGGCGGCGCACCACCGGCGTTCAACTCCTCTCTCGGTGCCAACATGCCTATGGTGGTGGACCATACATGCACAACTAGTGGGAGAGATTCGAAACCCAAAAGCCCCGAAGCTTTTCGCAACTCGGTCACATTGTCGATGCTTGAGGAGCTTGGTAATTACGGACATGCTCTTACCGATGCCGAGGAAGCAATTAGGCTCGAAACACTAATGTCAAGGTGGGTCTATGAAGGAGTCATTGATGATCGTTATGGTGAATGTTTCATTGCTGAGAACAAATCTCTTCAAAAGATGCTGTTTAAGGGTTTTCCCGATGCTTGTGATTCATTGATGGTTCCGAGGAATGGTGCACTGGACATAAAGACAGAGCCTTTATGGGAGAGGAGAGGCAACAGTCAATATACTCAAGCAGACATAGATATGATGCGAAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005654 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0005874 GO:0005881 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0007098 GO:0007275 GO:0008092 GO:0008104 GO:0008150 GO:0008275 GO:0009987 GO:0010638 GO:0010968 GO:0012505 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0030863 GO:0030981 GO:0031023 GO:0031109 GO:0031110 GO:0031112 GO:0031113 GO:0031116 GO:0031122 GO:0031334 GO:0031503 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032153 GO:0032155 GO:0032271 GO:0032273 GO:0032501 GO:0032502 GO:0032886 GO:0032991 GO:0033036 GO:0033043 GO:0033566 GO:0034613 GO:0034622 GO:0034629 GO:0043015 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044450 GO:0044464 GO:0046785 GO:0048229 GO:0048285 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051225 GO:0051258 GO:0051298 GO:0051321 GO:0051415 GO:0051418 GO:0051493 GO:0051495 GO:0051641 GO:0055028 GO:0065003 GO:0065007 GO:0070013 GO:0070507 GO:0070727 GO:0070925 GO:0071840 GO:0071944 GO:0090063 GO:0090307 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902850 GO:1902903 GO:1902905 GO:1903047
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spc97_Spc98

Protein Analysis

177

Amino Acids

19.6

Weight (kDa)

5.96

Isoelectric Point (pI)

57.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 134
AciI CCGC 1 cut(s) 102
AcoI YGGCCR 1 cut(s) 70
AfiI CCNNNNNNNGG 1 cut(s) 440
AgsI TTSAA 3 cut(s) 56, 121, 383
AhdI GACNNNNNGTC 1 cut(s) 20
AhlI ACTAGT 1 cut(s) 173
AluBI AGCT 3 cut(s) 30, 209, 247
AluI AGCT 3 cut(s) 30, 209, 247
Alw21I GWGCWC 1 cut(s) 448
Alw26I GTCTC 1 cut(s) 58
Alw44I GTGCAC 1 cut(s) 444
Ama87I CYCGRG 1 cut(s) 25
AoxI GGCC 1 cut(s) 70
ApaLI GTGCAC 1 cut(s) 444
Asp700I GAANNNNTTC 1 cut(s) 210
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 3 cut(s) 22, 85, 157
AsuC2I CCSGG 2 cut(s) 12, 69
AsuHPI GGTGA 1 cut(s) 362
AsuII TTCGAA 1 cut(s) 188
AvaI CYCGRG 1 cut(s) 25
AvaII GGWCC 3 cut(s) 22, 85, 157
BaeGI GKGCMC 1 cut(s) 448
BanI GGYRCC 1 cut(s) 134
Bbv12I GWGCWC 1 cut(s) 448
BccI CCATC 1 cut(s) 421
BcnI CCSGG 2 cut(s) 12, 69
BcoDI GTCTC 1 cut(s) 58
BcuI ACTAGT 1 cut(s) 173
BfaI CTAG 1 cut(s) 174
BisI GCNGC 1 cut(s) 103
BlsI GCNGC 1 cut(s) 104
Bme1390I CCNGG 2 cut(s) 12, 69
Bme18I GGWCC 3 cut(s) 22, 85, 157
BmeRI GACNNNNNGTC 1 cut(s) 20
BmeT110I CYCGRG 1 cut(s) 25
BmgT120I GGNCC 3 cut(s) 22, 85, 157
BmiI GGNNCC 3 cut(s) 24, 136, 432
BmrFI CCNGG 2 cut(s) 12, 69
BmsI GCATC 6 cut(s) 101, 225, 265, 378, 400, 510
BplI GAGNNNNNCTC 2 cut(s) 81, 113
Bpu14I TTCGAA 1 cut(s) 188
BpuEI CTTGAG 2 cut(s) 260, 486
BpuMI CCSGG 2 cut(s) 12, 69
BsaI GGTCTC 1 cut(s) 58
BsaJI CCNNGG 3 cut(s) 81, 279, 434
Bsc4I CCNNNNNNNGG 1 cut(s) 440
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 1 cut(s) 453
Bse3DI GCAATG 1 cut(s) 360
BseDI CCNNGG 3 cut(s) 81, 279, 434
BseLI CCNNNNNNNGG 1 cut(s) 440
BseMI GCAATG 1 cut(s) 360
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 1 cut(s) 453
BseRI GAGGAG 4 cut(s) 70, 115, 257, 493
BseSI GKGCMC 1 cut(s) 448
Bsh1285I CGRYCG 1 cut(s) 11
BshFI GGCC 1 cut(s) 72
BshNI GGYRCC 1 cut(s) 134
BsiEI CGRYCG 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 448
BsiHKCI CYCGRG 1 cut(s) 25
BsiSI CCGG 3 cut(s) 11, 69, 113
BslFI GGGAC 2 cut(s) 8, 27
BslI CCNNNNNNNGG 1 cut(s) 440
BsmAI GTCTC 1 cut(s) 58
BsmFI GGGAC 2 cut(s) 8, 27
BsnI GGCC 1 cut(s) 72
Bso31I GGTCTC 1 cut(s) 58
BsoBI CYCGRG 1 cut(s) 25
Bsp119I TTCGAA 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 448
Bsp143I GATC 1 cut(s) 340
BspACI CCGC 1 cut(s) 102
BspANI GGCC 1 cut(s) 72
BspCNI CTCAG 1 cut(s) 358
BspLI GGNNCC 3 cut(s) 24, 136, 432
BspT104I TTCGAA 1 cut(s) 188
BspT107I GGYRCC 1 cut(s) 134
BspTNI GGTCTC 1 cut(s) 58
BsrDI GCAATG 1 cut(s) 360
BsrFI RCCGGY 1 cut(s) 112
BsrI ACTGG 1 cut(s) 453
BssAI RCCGGY 1 cut(s) 112
BssECI CCNNGG 3 cut(s) 81, 279, 434
BssMI GATC 1 cut(s) 340
Bst4CI ACNGT 1 cut(s) 491
Bst6I CTCTTC 1 cut(s) 384
BstBI TTCGAA 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 32
BstDEI CTNAG 1 cut(s) 366
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 1 cut(s) 343
BstMAI GTCTC 1 cut(s) 58
BstMBI GATC 1 cut(s) 340
BstMCI CGRYCG 1 cut(s) 11
BstMWI GCNNNNNNNGC 3 cut(s) 113, 244, 284
BstNSI RCATGY 3 cut(s) 145, 168, 266
BstSCI CCNGG 2 cut(s) 10, 67
BstSLI GKGCMC 1 cut(s) 448
BsuRI GGCC 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 446
Cac8I GCNNGC 1 cut(s) 32
CfoI GCGC 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 3 cut(s) 22, 85, 157
CpoI CGGWCCG 1 cut(s) 85
CspI CGGWCCG 1 cut(s) 85
CviAII CATG 3 cut(s) 142, 165, 263
CviJI RGCY 7 cut(s) 30, 72, 201, 209, 247, 295, 466
CviKI_1 RGCY 7 cut(s) 30, 72, 201, 209, 247, 295, 466
DdeI CTNAG 1 cut(s) 366
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
DriI GACNNNNNGTC 1 cut(s) 20
EaeI YGGCCR 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 384
Eam1105I GACNNNNNGTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 384
Eco31I GGTCTC 1 cut(s) 58
Eco47I GGWCC 3 cut(s) 22, 85, 157
Eco88I CYCGRG 1 cut(s) 25
FaeI CATG 3 cut(s) 145, 168, 266
FaqI GGGAC 2 cut(s) 8, 27
FatI CATG 3 cut(s) 141, 164, 262
Fnu4HI GCNGC 1 cut(s) 103
Fsp4HI GCNGC 1 cut(s) 103
FspBI CTAG 1 cut(s) 174
GlaI GCGC 1 cut(s) 106
GluI GCNGC 1 cut(s) 103
HaeIII GGCC 1 cut(s) 72
HapII CCGG 3 cut(s) 11, 69, 113
HhaI GCGC 1 cut(s) 107
Hin1II CATG 3 cut(s) 145, 168, 266
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HindIII AAGCTT 1 cut(s) 207
HinfI GANTC 4 cut(s) 15, 185, 330, 419
HpaII CCGG 3 cut(s) 11, 69, 113
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 2 cut(s) 157, 446
Hpy188I TCNGA 2 cut(s) 89, 435
Hpy188III TCNNGA 2 cut(s) 25, 407
Hpy8I GTNNAC 2 cut(s) 157, 446
HpyAV CCTTC 1 cut(s) 320
HpyCH4III ACNGT 1 cut(s) 491
HpyCH4V TGCA 2 cut(s) 168, 446
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 244, 284
HpyF3I CTNAG 1 cut(s) 366
Hsp92II CATG 3 cut(s) 145, 168, 266
HspAI GCGC 1 cut(s) 105
Kzo9I GATC 1 cut(s) 340
LmnI GCTCC 1 cut(s) 244
LpnPI CCDG 4 cut(s) 24, 82, 126, 434
LweI GCATC 6 cut(s) 101, 225, 265, 378, 400, 510
MaeI CTAG 1 cut(s) 174
MaeIII GTNAC 1 cut(s) 223
MalI GATC 1 cut(s) 342
MboI GATC 1 cut(s) 340
MboII GAAGA 1 cut(s) 371
MhlI GDGCHC 1 cut(s) 448
MluCI AATT 2 cut(s) 253, 288
MlyI GAGTC 2 cut(s) 9, 339
MnlI CCTC 7 cut(s) 91, 136, 235, 274, 429, 471, 476
MroXI GAANNNNTTC 1 cut(s) 210
MseI TTAA 1 cut(s) 396
MslI CAYNNNNRTG 2 cut(s) 146, 515
MspI CCGG 3 cut(s) 11, 69, 113
MspR9I CCNGG 2 cut(s) 12, 69
MwoI GCNNNNNNNGC 3 cut(s) 113, 244, 284
NciI CCSGG 2 cut(s) 12, 69
NdeII GATC 1 cut(s) 340
NlaIII CATG 3 cut(s) 145, 168, 266
NlaIV GGNNCC 3 cut(s) 24, 136, 432
NmeAIII GCCGAG 2 cut(s) 78, 304
NmuCI GTSAC 1 cut(s) 223
NspI RCATGY 3 cut(s) 145, 168, 266
NspV TTCGAA 1 cut(s) 188
PdmI GAANNNNTTC 1 cut(s) 210
PfeI GAWTC 2 cut(s) 185, 419
PkrI GCNGC 1 cut(s) 104
PleI GAGTC 2 cut(s) 9, 338
PpsI GAGTC 2 cut(s) 9, 338
PspN4I GGNNCC 3 cut(s) 24, 136, 432
PspPI GGNCC 3 cut(s) 22, 85, 157
RseI CAYNNNNRTG 2 cut(s) 146, 515
Rsr2I CGGWCCG 1 cut(s) 85
RsrII CGGWCCG 1 cut(s) 85
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 1 cut(s) 103
Sau3AI GATC 1 cut(s) 340
Sau96I GGNCC 3 cut(s) 22, 85, 157
SchI GAGTC 2 cut(s) 9, 339
ScrFI CCNGG 2 cut(s) 12, 69
SduI GDGCHC 1 cut(s) 448
SetI ASST 4 cut(s) 32, 211, 249, 317
SfaNI GCATC 6 cut(s) 101, 225, 265, 378, 400, 510
SfuI TTCGAA 1 cut(s) 188
SgrAI CRCCGGYG 1 cut(s) 112
SinI GGWCC 3 cut(s) 22, 85, 157
SmiMI CAYNNNNRTG 2 cut(s) 146, 515
SmlI CTYRAG 2 cut(s) 239, 501
SmoI CTYRAG 2 cut(s) 239, 501
SpeI ACTAGT 1 cut(s) 173
Sse9I AATT 2 cut(s) 253, 288
SsiI CCGC 1 cut(s) 102
SspMI CTAG 1 cut(s) 174
StyD4I CCNGG 2 cut(s) 10, 67
TaaI ACNGT 1 cut(s) 491
TaqI TCGA 4 cut(s) 7, 188, 233, 297
TaqII GACCGA 3 cut(s) 10, 73, 211
TasI AATT 2 cut(s) 253, 288
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 2 cut(s) 185, 419
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TscAI CASTG 1 cut(s) 453
TseFI GTSAC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 3 cut(s) 339, 349, 411
TspGWI ACGGA 1 cut(s) 273
TspRI CASTG 1 cut(s) 453
VneI GTGCAC 1 cut(s) 444
VpaK11BI GGWCC 3 cut(s) 22, 85, 157
XceI RCATGY 3 cut(s) 145, 168, 266
XcmI CCANNNNNNNNNTGG 1 cut(s) 145
XmnI GAANNNNTTC 1 cut(s) 210
XspI CTAG 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.