Rorug07G0130400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0015770
Physical Location & Seq
Reverse (-)
10310934 .. 10313254
2321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0130400.1

Sequence Viewer

Length: 885 bp
ATGGATGCCCTGAAAGGAGGCAAGCCAGCCATGAAGAAATCCTCCTCCATCAACTCCGACGACTCCGCCTGCACCGCCGGCTCCCCCCTCCGCTACCTCTCCCCCATGCGATCCGACGCCGGCGACCCCTCCGAATCCCCCCCCTACGGCTCCCCGGAGCTCTCCCCCCAGAAGCCGCCCCCCGACAACTCCAAGGCCATCATGGCCGTCGACAAGTTCACGCAGTACTCCCCCTCCCCCTTCCGCCCTCAGAAACCGCCCGAGAAGGCGCCCTCGCCGCTCGTCGTCTACAACAGCAACCGCGCCGCCAGGGAGGACCCCCCGCCCTCGGTGCCCAAGGCGGCCGCTCCTAACGGCGTGCCCTGGACTGCCGGAGGCGGCGCCGGGGTTGGGAGATCGAGGCGGCCGCCGAGGAGAGACGATGTGTTGACGACGGCCGCGCTGGGGTTTCGGGTTAGCGAGTTGGTGCTATGCTTGATTTCGTTCTCGGTTATGGCCGCTGATAAAACTCAGGGCTGGAGCGGCGATTCCTTTGATCGCTACATGGAATACAGGTATTGTTTAGCTGTGACTGTTATTGGATTTGCATATGCTGCTTTTCAAGCATACAATCTATCCTACCATCTAGTCACCGGGAATTATGTGATCAGGCACCACCTTCGTCACCACTTTGATTTCTTCATGGATCAGGTACTGGCATATCTTCTCATTTCAGCATCATCATCGGCAGCCACCAGGGTCGACGACTGGCAATCAAATTGGGGAAAAGATGAGTTCACGGAGATGGCTACTGCTTCAGTCAGCATGGCCTTCCTCGCTTTCATTGCCTTTGCACTAAGCTCCCTGATCTCTGGTTACAACCTATGTACCCATGATTCTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

294

Amino Acids

31.93

Weight (kDa)

7.68

Isoelectric Point (pI)

56.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 142 - 276 1.1e-30 Casparian strip membrane protein domain
MARVEL PF01284 144 - 280 7e-07 Membrane-associating domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 268, 331, 380, 651
AccBSI CCGCTC 3 cut(s) 280, 347, 522
AccI GTMKAC 3 cut(s) 210, 288, 741
AccII CGCG 2 cut(s) 303, 440
AclWI GGATC 2 cut(s) 105, 693
AcoI YGGCCR 5 cut(s) 204, 342, 404, 435, 495
AcuI CTGAAG 1 cut(s) 780
AcyI GRCGYC 3 cut(s) 117, 269, 381
AfaI GTAC 3 cut(s) 227, 693, 868
AfiI CCNNNNNNNGG 4 cut(s) 146, 328, 390, 444
AgsI TTSAA 1 cut(s) 602
AjnI CCWGG 3 cut(s) 308, 362, 734
AluBI AGCT 3 cut(s) 160, 566, 840
AluI AGCT 3 cut(s) 160, 566, 840
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 411
AlwI GGATC 2 cut(s) 105, 693
AlwNI CAGNNNCTG 1 cut(s) 694
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 7 cut(s) 195, 204, 342, 404, 435, 495, 807
ApeKI GCWGC 2 cut(s) 593, 728
AspLEI GCGC 4 cut(s) 271, 305, 383, 442
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 3 cut(s) 155, 385, 634
AsuHPI GGTGA 2 cut(s) 622, 656
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 1 cut(s) 316
BaeGI GKGCMC 2 cut(s) 336, 363
BaeI ACNNNNGTAYC 2 cut(s) 850, 883
BanI GGYRCC 4 cut(s) 268, 331, 380, 651
BanII GRGCYC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 2 cut(s) 580, 740
BccI CCATC 4 cut(s) 56, 206, 630, 778
BceAI ACGGC 4 cut(s) 163, 191, 370, 450
BcgI CGANNNNNNTGC 4 cut(s) 641, 675, 705, 739
BciT130I CCWGG 3 cut(s) 310, 364, 736
BclI TGATCA 1 cut(s) 645
BcnI CCSGG 3 cut(s) 155, 385, 634
BcoDI GTCTC 1 cut(s) 411
BfaI CTAG 1 cut(s) 626
BfoI RGCGCY 2 cut(s) 272, 384
BglI GCCNNNNNGGC 1 cut(s) 203
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 6 cut(s) 155, 310, 364, 385, 634, 736
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 7 cut(s) 82, 151, 270, 318, 333, 382, 653
BmrFI CCNGG 6 cut(s) 155, 310, 364, 385, 634, 736
BmsI GCATC 1 cut(s) 725
BpmI CTGGAG 1 cut(s) 538
BpuMI CCSGG 3 cut(s) 155, 385, 634
BsaAI YACGTR 1 cut(s) 882
BsaHI GRCGYC 3 cut(s) 117, 269, 381
BsaJI CCNNGG 9 cut(s) 153, 192, 309, 327, 336, 362, 384, 410, 735
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 4 cut(s) 146, 328, 390, 444
Bse118I RCCGGY 2 cut(s) 77, 119
Bse1I ACTGG 2 cut(s) 699, 752
Bse3DI GCAATG 1 cut(s) 822
BseBI CCWGG 3 cut(s) 310, 364, 736
BseDI CCNNGG 9 cut(s) 153, 192, 309, 327, 336, 362, 384, 410, 735
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 4 cut(s) 146, 328, 390, 444
BseMI GCAATG 1 cut(s) 822
BseMII CTCAG 2 cut(s) 263, 524
BseNI ACTGG 2 cut(s) 699, 752
BseRI GAGGAG 2 cut(s) 34, 427
BseSI GKGCMC 2 cut(s) 336, 363
BseX3I CGGCCG 3 cut(s) 342, 404, 435
BseXI GCAGC 2 cut(s) 580, 740
BseYI CCCAGC 1 cut(s) 442
BsgI GTGCAG 1 cut(s) 55
Bsh1236I CGCG 2 cut(s) 303, 440
Bsh1285I CGRYCG 3 cut(s) 345, 407, 438
BshFI GGCC 7 cut(s) 197, 206, 344, 406, 437, 497, 809
BshNI GGYRCC 4 cut(s) 268, 331, 380, 651
BsiEI CGRYCG 3 cut(s) 345, 407, 438
BsiHKAI GWGCWC 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 6 cut(s) 78, 120, 155, 372, 384, 633
BslI CCNNNNNNNGG 4 cut(s) 146, 328, 390, 444
BsmAI GTCTC 1 cut(s) 411
BsmBI CGTCTC 1 cut(s) 411
BsnI GGCC 7 cut(s) 197, 206, 344, 406, 437, 497, 809
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 3 cut(s) 162, 336, 363
Bsp143I GATC 6 cut(s) 110, 395, 535, 645, 685, 846
BspANI GGCC 7 cut(s) 197, 206, 344, 406, 437, 497, 809
BspCNI CTCAG 2 cut(s) 262, 523
BspFNI CGCG 2 cut(s) 303, 440
BspLI GGNNCC 7 cut(s) 82, 151, 270, 318, 333, 382, 653
BspPI GGATC 2 cut(s) 105, 693
BspT107I GGYRCC 4 cut(s) 268, 331, 380, 651
BsrBI CCGCTC 3 cut(s) 280, 347, 522
BsrDI GCAATG 1 cut(s) 822
BsrFI RCCGGY 2 cut(s) 77, 119
BsrI ACTGG 2 cut(s) 699, 752
BssAI RCCGGY 2 cut(s) 77, 119
BssECI CCNNGG 9 cut(s) 153, 192, 309, 327, 336, 362, 384, 410, 735
BssMI GATC 6 cut(s) 110, 395, 535, 645, 685, 846
BssNI GRCGYC 3 cut(s) 117, 269, 381
BssT1I CCWWGG 2 cut(s) 192, 336
Bst2UI CCWGG 3 cut(s) 310, 364, 736
Bst4CI ACNGT 1 cut(s) 574
BstACI GRCGYC 3 cut(s) 117, 269, 381
BstAPI GCANNNNNTGC 1 cut(s) 593
BstBAI YACGTR 1 cut(s) 882
BstC8I GCNNGC 6 cut(s) 23, 27, 70, 79, 121, 359
BstDEI CTNAG 3 cut(s) 249, 510, 836
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 2 cut(s) 303, 440
BstH2I RGCGCY 2 cut(s) 272, 384
BstHHI GCGC 4 cut(s) 271, 305, 383, 442
BstKTI GATC 6 cut(s) 113, 398, 538, 648, 688, 849
BstMAI GTCTC 1 cut(s) 411
BstMBI GATC 6 cut(s) 110, 395, 535, 645, 685, 846
BstMCI CGRYCG 3 cut(s) 345, 407, 438
BstNI CCWGG 3 cut(s) 310, 364, 736
BstSCI CCNGG 6 cut(s) 153, 308, 362, 383, 632, 734
BstSLI GKGCMC 2 cut(s) 336, 363
BstUI CGCG 2 cut(s) 303, 440
BstV1I GCAGC 2 cut(s) 580, 740
BstZI CGGCCG 3 cut(s) 342, 404, 435
BsuRI GGCC 7 cut(s) 197, 206, 344, 406, 437, 497, 809
BtsCI GGATG 1 cut(s) 10
Cac8I GCNNGC 6 cut(s) 23, 27, 70, 79, 121, 359
CaiI CAGNNNCTG 1 cut(s) 694
CciNI GCGGCCGC 2 cut(s) 342, 404
CfoI GCGC 4 cut(s) 271, 305, 383, 442
Cfr10I RCCGGY 2 cut(s) 77, 119
Cfr13I GGNCC 1 cut(s) 316
CseI GACGC 1 cut(s) 125
Csp6I GTAC 3 cut(s) 226, 692, 867
CviAII CATG 7 cut(s) 31, 106, 202, 544, 682, 805, 872
CviQI GTAC 3 cut(s) 226, 692, 867
DdeI CTNAG 3 cut(s) 249, 510, 836
DinI GGCGCC 2 cut(s) 270, 382
DpnI GATC 6 cut(s) 112, 397, 537, 647, 687, 848
DpnII GATC 6 cut(s) 110, 395, 535, 645, 685, 846
EaeI YGGCCR 5 cut(s) 204, 342, 404, 435, 495
EagI CGGCCG 3 cut(s) 342, 404, 435
EciI GGCGGA 2 cut(s) 55, 233
Ecl136II GAGCTC 1 cut(s) 160
EclXI CGGCCG 3 cut(s) 342, 404, 435
Eco130I CCWWGG 2 cut(s) 192, 336
Eco24I GRGCYC 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 316
Eco52I CGGCCG 3 cut(s) 342, 404, 435
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 780
Eco88I CYCGRG 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 316
EcoRII CCWGG 3 cut(s) 308, 362, 734
EcoT14I CCWWGG 2 cut(s) 192, 336
EcoT38I GRGCYC 1 cut(s) 162
EgeI GGCGCC 2 cut(s) 270, 382
EheI GGCGCC 2 cut(s) 270, 382
ErhI CCWWGG 2 cut(s) 192, 336
Esp3I CGTCTC 1 cut(s) 411
FaeI CATG 7 cut(s) 34, 109, 205, 547, 685, 808, 875
FatI CATG 7 cut(s) 30, 105, 201, 543, 681, 804, 871
FauI CCCGC 1 cut(s) 330
FauNDI CATATG 1 cut(s) 589
FbaI TGATCA 1 cut(s) 645
FblI GTMKAC 3 cut(s) 210, 288, 741
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 162
FspBI CTAG 1 cut(s) 626
GlaI GCGC 4 cut(s) 270, 304, 382, 441
GsaI CCCAGC 1 cut(s) 446
GsuI CTGGAG 1 cut(s) 538
HaeII RGCGCY 2 cut(s) 272, 384
HaeIII GGCC 7 cut(s) 197, 206, 344, 406, 437, 497, 809
HapII CCGG 6 cut(s) 78, 120, 155, 372, 384, 633
HgaI GACGC 1 cut(s) 125
HhaI GCGC 4 cut(s) 271, 305, 383, 442
Hin1I GRCGYC 3 cut(s) 117, 269, 381
Hin1II CATG 7 cut(s) 34, 109, 205, 547, 685, 808, 875
Hin6I GCGC 4 cut(s) 269, 303, 381, 440
HinP1I GCGC 4 cut(s) 269, 303, 381, 440
HincII GTYRAC 3 cut(s) 211, 429, 742
HindII GTYRAC 3 cut(s) 211, 429, 742
HinfI GANTC 4 cut(s) 62, 134, 527, 875
HpaII CCGG 6 cut(s) 78, 120, 155, 372, 384, 633
HphI GGTGA 2 cut(s) 622, 656
Hpy166II GTNNAC 6 cut(s) 211, 219, 289, 429, 742, 777
Hpy188I TCNGA 4 cut(s) 58, 115, 133, 252
Hpy8I GTNNAC 6 cut(s) 211, 219, 289, 429, 742, 777
Hpy99I CGWCG 6 cut(s) 62, 119, 212, 287, 436, 746
HpyAV CCTTC 4 cut(s) 250, 259, 668, 820
HpyCH4III ACNGT 1 cut(s) 574
HpyCH4IV ACGT 1 cut(s) 881
HpyCH4V TGCA 3 cut(s) 72, 587, 833
HpyF3I CTNAG 3 cut(s) 249, 510, 836
HpySE526I ACGT 1 cut(s) 881
Hsp92I GRCGYC 3 cut(s) 117, 269, 381
Hsp92II CATG 7 cut(s) 34, 109, 205, 547, 685, 808, 875
HspAI GCGC 4 cut(s) 269, 303, 381, 440
KasI GGCGCC 2 cut(s) 268, 380
KroI GCCGGC 2 cut(s) 77, 119
KroNI GCCGGC 2 cut(s) 79, 121
Ksp22I TGATCA 1 cut(s) 645
Kzo9I GATC 6 cut(s) 110, 395, 535, 645, 685, 846
LmnI GCTCC 6 cut(s) 86, 155, 157, 352, 519, 845
Lsp1109I GCAGC 2 cut(s) 580, 740
LweI GCATC 1 cut(s) 725
MaeI CTAG 1 cut(s) 626
MaeII ACGT 1 cut(s) 881
MaeIII GTNAC 4 cut(s) 568, 628, 662, 854
MalI GATC 6 cut(s) 112, 397, 537, 647, 687, 848
MbiI CCGCTC 3 cut(s) 280, 347, 522
MboI GATC 6 cut(s) 110, 395, 535, 645, 685, 846
MboII GAAGA 3 cut(s) 46, 670, 695
MhlI GDGCHC 3 cut(s) 162, 336, 363
MluCI AATT 2 cut(s) 637, 757
Mly113I GGCGCC 2 cut(s) 269, 381
MlyI GAGTC 1 cut(s) 56
MmeI TCCRAC 2 cut(s) 81, 138
MreI CGCCGGCG 1 cut(s) 119
MroNI GCCGGC 2 cut(s) 77, 119
MslI CAYNNNNRTG 1 cut(s) 782
MspA1I CMGCKG 1 cut(s) 500
MspI CCGG 6 cut(s) 78, 120, 155, 372, 384, 633
MspR9I CCNGG 6 cut(s) 155, 310, 364, 385, 634, 736
MvaI CCWGG 3 cut(s) 310, 364, 736
MvnI CGCG 2 cut(s) 303, 440
NaeI GCCGGC 2 cut(s) 79, 121
NarI GGCGCC 2 cut(s) 269, 381
NciI CCSGG 3 cut(s) 155, 385, 634
NdeI CATATG 1 cut(s) 589
NdeII GATC 6 cut(s) 110, 395, 535, 645, 685, 846
NgoMIV GCCGGC 2 cut(s) 77, 119
NlaIII CATG 7 cut(s) 34, 109, 205, 547, 685, 808, 875
NlaIV GGNNCC 7 cut(s) 82, 151, 270, 318, 333, 382, 653
NmeAIII GCCGAG 1 cut(s) 435
NmuCI GTSAC 3 cut(s) 568, 628, 662
NotI GCGGCCGC 2 cut(s) 342, 404
PdiI GCCGGC 2 cut(s) 79, 121
PfeI GAWTC 3 cut(s) 134, 527, 875
PleI GAGTC 1 cut(s) 56
PluTI GGCGCC 2 cut(s) 272, 384
PpsI GAGTC 1 cut(s) 56
Ppu21I YACGTR 1 cut(s) 882
PpuMI RGGWCCY 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 162
Psp5II RGGWCCY 1 cut(s) 316
Psp6I CCWGG 3 cut(s) 308, 362, 734
PspFI CCCAGC 1 cut(s) 442
PspGI CCWGG 3 cut(s) 308, 362, 734
PspN4I GGNNCC 7 cut(s) 82, 151, 270, 318, 333, 382, 653
PspPI GGNCC 1 cut(s) 316
PspPPI RGGWCCY 1 cut(s) 316
PstNI CAGNNNCTG 1 cut(s) 694
RsaI GTAC 3 cut(s) 227, 693, 868
RsaNI GTAC 3 cut(s) 226, 692, 867
RseI CAYNNNNRTG 1 cut(s) 782
SacI GAGCTC 1 cut(s) 162
SalI GTCGAC 2 cut(s) 209, 740
Sau3AI GATC 6 cut(s) 110, 395, 535, 645, 685, 846
Sau96I GGNCC 1 cut(s) 316
ScaI AGTACT 1 cut(s) 227
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 6 cut(s) 155, 310, 364, 385, 634, 736
SduI GDGCHC 3 cut(s) 162, 336, 363
SetI ASST 9 cut(s) 99, 162, 557, 568, 660, 693, 842, 864, 884
SfaNI GCATC 1 cut(s) 725
SfiI GGCCNNNNNGGCC 1 cut(s) 203
SfoI GGCGCC 2 cut(s) 270, 382
SgrAI CRCCGGYG 1 cut(s) 119
SinI GGWCC 1 cut(s) 316
SmiMI CAYNNNNRTG 1 cut(s) 782
Sse9I AATT 2 cut(s) 637, 757
SspDI GGCGCC 2 cut(s) 268, 380
SspMI CTAG 1 cut(s) 626
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 6 cut(s) 153, 308, 362, 383, 632, 734
StyI CCWWGG 2 cut(s) 192, 336
TaaI ACNGT 1 cut(s) 574
TaiI ACGT 1 cut(s) 884
TaqI TCGA 3 cut(s) 210, 398, 741
TasI AATT 2 cut(s) 637, 757
TatI WGTACW 1 cut(s) 225
TfiI GAWTC 3 cut(s) 134, 527, 875
TseFI GTSAC 3 cut(s) 568, 628, 662
TseI GCWGC 2 cut(s) 593, 728
Tsp45I GTSAC 3 cut(s) 568, 628, 662
TspDTI ATGAA 3 cut(s) 47, 670, 811
TspGWI ACGGA 1 cut(s) 794
VpaK11BI GGWCC 1 cut(s) 316
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XmiI GTMKAC 3 cut(s) 210, 288, 741
XspI CTAG 1 cut(s) 626
ZrmI AGTACT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.