Rmu_sc0002832.1_g000014

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002832.1
OG0007825
Physical Location & Seq
Reverse (-)
80486 .. 85625
5140 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1251 bp
atgaagacgacgatgacgaagagcgtgaatgcggtggtagcagtcacgttggcgctgctaacgtgcgcgccttggtgcatttactcacagcagccgcaccagcttatggtgaacatgaccaccgtccgaaatgcccccgctctcggcgctttttgcttggatgggagtttgcctgcgtatcatattcacagaggcttcggcgccggagcaagaaactggcttttacagtttgagggtggagggtggtgtaatgatatagagtcttgcttggagagggcgaaaacaagaagaggttctacacgccttatgagcaagtgggaggtcttttctgggattctaagtaataatgcttctcttaatccagatttttacaattggaaccgtgtgaagattcgatattgtgatggagcttcatttgccggagacgcagtgtataagaatgggacaacgttgctctatttcagagggcaaaagatttgggaagccatcattcttgatcttctgcctaaaggtttaggaaaggcacgtaaggctttgttatcaggttgctctgctgggggtttagcagcatttttacattgtgacgacttcactaagtatgtaccaagtaatgccagtgtaaaatgcttgagtgatgctggattctttcttgatgaaagagacataggtttgaatcacacaatgaggtctttctataaagaccttgtctctctacagggtgtagaaaagaatctggataagaactgcaccggatcccttccttttcctgagttgtgtattttcccacagtatgcattgagattcatcacaccgccttttttcatcttgaactcggcttatgatgtttaccagttccatcatggattggtgccatcttcagctgatccacgcggacactggaaccgttgcaaactggatccggcagcatgcaatcctgctcagttagatgtattgcaaggtttcaggcgcgacatgcttgttgctttggcattgttctacaagtactcggcaagaggggggatgtacatcaactcatgctttgctcactgccaaagtgagtcccaggacacatggtttggggttgattctccaagaatacacaacaagaccattgcagaagctgtaggtgactggtattttagcagaaggataactaataaggaagttgactgtgcatacccttgtgacactacctgccataaccttataccagcaaacacaagctcaggtcttgtagaagatctcaattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

416

Amino Acids

46.33

Weight (kDa)

8.35

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 32 - 400 2.6e-161 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1202
AccB1I GGYRCC 2 cut(s) 200, 868
AccB7I CCANNNNNTGG 1 cut(s) 106
AccBSI CCGCTC 1 cut(s) 140
AccII CGCG 3 cut(s) 68, 891, 969
AciI CCGC 5 cut(s) 32, 95, 138, 812, 891
AclI AACGTT 1 cut(s) 449
AclWI GGATC 5 cut(s) 747, 760, 878, 911, 924
AcuI CTGAAG 1 cut(s) 861
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 3 cut(s) 603, 1004, 1025
AfiI CCNNNNNNNGG 2 cut(s) 106, 143
AgsI TTSAA 2 cut(s) 673, 829
AjnI CCWGG 1 cut(s) 1062
AluBI AGCT 5 cut(s) 103, 410, 881, 1121, 1224
AluI AGCT 5 cut(s) 103, 410, 881, 1121, 1224
Alw26I GTCTC 3 cut(s) 417, 654, 712
AlwI GGATC 5 cut(s) 747, 760, 878, 911, 924
AlwNI CAGNNNCTG 1 cut(s) 1121
ApeKI GCWGC 4 cut(s) 55, 91, 566, 923
ArsI GACNNNNNNTTYG 2 cut(s) 1058, 1090
Asp700I GAANNNNTTC 1 cut(s) 292
AspLEI GCGC 6 cut(s) 55, 68, 70, 149, 203, 969
AsuHPI GGTGA 2 cut(s) 121, 1139
BamHI GGATCC 2 cut(s) 752, 916
BanI GGYRCC 2 cut(s) 200, 868
BarI GAAGNNNNNNTAC 4 cut(s) 280, 312, 334, 366
BbsI GAAGAC 1 cut(s) 11
BbvI GCAGC 4 cut(s) 42, 103, 578, 935
BccI CCATC 5 cut(s) 155, 398, 494, 864, 880
BciT130I CCWGG 1 cut(s) 1064
BcoDI GTCTC 3 cut(s) 417, 654, 712
BfmI CTRYAG 2 cut(s) 713, 1122
BfoI RGCGCY 3 cut(s) 56, 150, 204
BfuAI ACCTGC 1 cut(s) 1202
BglII AGATCT 1 cut(s) 1240
BisI GCNGC 5 cut(s) 56, 92, 95, 567, 924
BlsI GCNGC 5 cut(s) 57, 93, 96, 568, 925
BmcAI AGTACT 1 cut(s) 1004
Bme1390I CCNGG 1 cut(s) 1064
BmiI GGNNCC 6 cut(s) 202, 380, 754, 870, 902, 918
BmrFI CCNGG 1 cut(s) 1064
BmsI GCATC 1 cut(s) 625
BoxI GACNNNNGTC 1 cut(s) 122
BpiI GAAGAC 1 cut(s) 11
Bpu10I CCTNAGC 1 cut(s) 1225
BpuEI CTTGAG 1 cut(s) 649
BsaAI YACGTR 1 cut(s) 527
BsaBI GATNNNNATC 1 cut(s) 1025
BsaHI GRCGYC 1 cut(s) 201
BsaJI CCNNGG 2 cut(s) 71, 1062
BsaWI WCCGGW 1 cut(s) 749
BsaXI ACNNNNNCTCC 2 cut(s) 414, 444
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 143
Bse1I ACTGG 6 cut(s) 221, 615, 850, 902, 918, 1136
Bse3DI GCAATG 1 cut(s) 1110
Bse8I GATNNNNATC 1 cut(s) 1025
BseBI CCWGG 1 cut(s) 1064
BseDI CCNNGG 2 cut(s) 71, 1062
BseGI GGATG 2 cut(s) 166, 1026
BseJI GATNNNNATC 1 cut(s) 1025
BseLI CCNNNNNNNGG 2 cut(s) 106, 143
BseMI GCAATG 1 cut(s) 1110
BseMII CTCAG 3 cut(s) 759, 953, 1239
BseNI ACTGG 6 cut(s) 221, 615, 850, 902, 918, 1136
BsePI GCGCGC 1 cut(s) 66
BseXI GCAGC 4 cut(s) 42, 103, 578, 935
BseYI CCCAGC 1 cut(s) 554
BsgI GTGCAG 1 cut(s) 730
Bsh1236I CGCG 3 cut(s) 68, 891, 969
BshNI GGYRCC 2 cut(s) 200, 868
BsiSI CCGG 4 cut(s) 204, 420, 750, 920
BslFI GGGAC 2 cut(s) 457, 1045
BslI CCNNNNNNNGG 2 cut(s) 106, 143
BsmAI GTCTC 3 cut(s) 417, 654, 712
BsmBI CGTCTC 1 cut(s) 417
BsmFI GGGAC 2 cut(s) 457, 1045
BsmI GAATGC 1 cut(s) 34
Bsp1407I TGTACA 1 cut(s) 1023
Bsp143I GATC 5 cut(s) 496, 752, 883, 916, 1240
BspACI CCGC 5 cut(s) 32, 95, 138, 812, 891
BspCNI CTCAG 3 cut(s) 760, 952, 1238
BspFNI CGCG 3 cut(s) 68, 891, 969
BspLI GGNNCC 6 cut(s) 202, 380, 754, 870, 902, 918
BspMI ACCTGC 1 cut(s) 1202
BspPI GGATC 5 cut(s) 747, 760, 878, 911, 924
BspQI GCTCTTC 1 cut(s) 14
BspT107I GGYRCC 2 cut(s) 200, 868
BsrBI CCGCTC 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 1110
BsrGI TGTACA 1 cut(s) 1023
BsrI ACTGG 6 cut(s) 221, 615, 850, 902, 918, 1136
BssECI CCNNGG 2 cut(s) 71, 1062
BssHII GCGCGC 1 cut(s) 66
BssMI GATC 5 cut(s) 496, 752, 883, 916, 1240
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 71
Bst2UI CCWGG 1 cut(s) 1064
Bst4CI ACNGT 6 cut(s) 124, 228, 383, 789, 905, 1172
Bst6I CTCTTC 2 cut(s) 14, 283
BstACI GRCGYC 1 cut(s) 201
BstAUI TGTACA 1 cut(s) 1023
BstBAI YACGTR 1 cut(s) 527
BstC8I GCNNGC 3 cut(s) 68, 174, 928
BstDEI CTNAG 5 cut(s) 338, 594, 768, 939, 1225
BstF5I GGATG 2 cut(s) 166, 1026
BstFNI CGCG 3 cut(s) 68, 891, 969
BstH2I RGCGCY 3 cut(s) 56, 150, 204
BstHHI GCGC 6 cut(s) 55, 68, 70, 149, 203, 969
BstKTI GATC 5 cut(s) 499, 755, 886, 919, 1243
BstMAI GTCTC 3 cut(s) 417, 654, 712
BstMBI GATC 5 cut(s) 496, 752, 883, 916, 1240
BstMWI GCNNNNNNNGC 9 cut(s) 38, 100, 146, 153, 309, 416, 425, 530, 973
BstNI CCWGG 1 cut(s) 1064
BstNSI RCATGY 2 cut(s) 930, 976
BstPAI GACNNNNGTC 1 cut(s) 122
BstSCI CCNGG 1 cut(s) 1062
BstSFI CTRYAG 2 cut(s) 713, 1122
BstUI CGCG 3 cut(s) 68, 891, 969
BstV1I GCAGC 4 cut(s) 42, 103, 578, 935
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 3 cut(s) 752, 916, 1240
BstYI RGATCY 3 cut(s) 752, 916, 1240
BtsCI GGATG 2 cut(s) 166, 1026
BtsI GCAGTG 2 cut(s) 435, 1045
BtsIMutI CAGTG 4 cut(s) 435, 622, 895, 1045
BveI ACCTGC 1 cut(s) 1202
Cac8I GCNNGC 3 cut(s) 68, 174, 928
CaiI CAGNNNCTG 1 cut(s) 1121
CfoI GCGC 6 cut(s) 55, 68, 70, 149, 203, 969
CseI GACGC 1 cut(s) 434
Csp6I GTAC 3 cut(s) 602, 1003, 1024
CviAII CATG 6 cut(s) 115, 860, 927, 973, 1035, 1071
CviQI GTAC 3 cut(s) 602, 1003, 1024
DdeI CTNAG 5 cut(s) 338, 594, 768, 939, 1225
DinI GGCGCC 1 cut(s) 202
DpnI GATC 5 cut(s) 498, 754, 885, 918, 1242
DpnII GATC 5 cut(s) 496, 752, 883, 916, 1240
Eam1104I CTCTTC 2 cut(s) 14, 283
EarI CTCTTC 2 cut(s) 14, 283
Eco130I CCWWGG 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 861
EcoRII CCWGG 1 cut(s) 1062
EcoT14I CCWWGG 1 cut(s) 71
EcoT22I ATGCAT 1 cut(s) 796
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
ErhI CCWWGG 1 cut(s) 71
Esp3I CGTCTC 1 cut(s) 417
FaeI CATG 6 cut(s) 118, 863, 930, 976, 1038, 1074
FaqI GGGAC 2 cut(s) 457, 1045
FatI CATG 6 cut(s) 114, 859, 926, 972, 1034, 1070
FauI CCCGC 1 cut(s) 145
Fnu4HI GCNGC 5 cut(s) 56, 92, 95, 567, 924
FokI GGATG 2 cut(s) 173, 1033
Fsp4HI GCNGC 5 cut(s) 56, 92, 95, 567, 924
GlaI GCGC 6 cut(s) 54, 67, 69, 148, 202, 968
GluI GCNGC 5 cut(s) 56, 92, 95, 567, 924
GsaI CCCAGC 1 cut(s) 558
HaeII RGCGCY 3 cut(s) 56, 150, 204
HapII CCGG 4 cut(s) 204, 420, 750, 920
HgaI GACGC 1 cut(s) 434
HhaI GCGC 6 cut(s) 55, 68, 70, 149, 203, 969
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 6 cut(s) 118, 863, 930, 976, 1038, 1074
Hin6I GCGC 6 cut(s) 53, 66, 68, 147, 201, 967
HinP1I GCGC 6 cut(s) 53, 66, 68, 147, 201, 967
HincII GTYRAC 1 cut(s) 1168
HindII GTYRAC 1 cut(s) 1168
HinfI GANTC 9 cut(s) 260, 334, 391, 642, 673, 730, 801, 1058, 1085
HpaII CCGG 4 cut(s) 204, 420, 750, 920
HphI GGTGA 2 cut(s) 121, 1139
Hpy166II GTNNAC 3 cut(s) 112, 847, 1168
Hpy188I TCNGA 2 cut(s) 128, 464
Hpy188III TCNNGA 6 cut(s) 362, 494, 650, 734, 767, 826
Hpy8I GTNNAC 3 cut(s) 112, 847, 1168
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 2 cut(s) 767, 1140
HpyCH4III ACNGT 6 cut(s) 124, 228, 383, 789, 905, 1172
HpyCH4IV ACGT 4 cut(s) 47, 62, 449, 526
HpyCH4V TGCA 8 cut(s) 78, 747, 794, 909, 930, 955, 1115, 1175
HpyF10VI GCNNNNNNNGC 9 cut(s) 38, 100, 146, 153, 309, 416, 425, 530, 973
HpyF3I CTNAG 5 cut(s) 338, 594, 768, 939, 1225
HpySE526I ACGT 4 cut(s) 47, 62, 449, 526
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 6 cut(s) 118, 863, 930, 976, 1038, 1074
HspAI GCGC 6 cut(s) 53, 66, 68, 147, 201, 967
KasI GGCGCC 1 cut(s) 200
Kzo9I GATC 5 cut(s) 496, 752, 883, 916, 1240
LguI GCTCTTC 1 cut(s) 14
LmnI GCTCC 2 cut(s) 206, 407
Lsp1109I GCAGC 4 cut(s) 42, 103, 578, 935
LweI GCATC 1 cut(s) 625
MaeII ACGT 4 cut(s) 47, 62, 449, 526
MaeIII GTNAC 4 cut(s) 43, 581, 1127, 1184
MalI GATC 5 cut(s) 498, 754, 885, 918, 1242
MbiI CCGCTC 1 cut(s) 140
MboI GATC 5 cut(s) 496, 752, 883, 916, 1240
MboII GAAGA 7 cut(s) 16, 31, 300, 400, 491, 867, 1250
MfeI CAATTG 1 cut(s) 373
MflI RGATCY 3 cut(s) 752, 916, 1240
MluCI AATT 2 cut(s) 373, 1246
Mly113I GGCGCC 1 cut(s) 201
MlyI GAGTC 2 cut(s) 269, 1067
MnlI CCTC 9 cut(s) 185, 226, 233, 267, 284, 313, 458, 678, 1007
Mph1103I ATGCAT 1 cut(s) 796
MroXI GAANNNNTTC 1 cut(s) 292
MseI TTAA 2 cut(s) 357, 1249
MspA1I CMGCKG 1 cut(s) 881
MspI CCGG 4 cut(s) 204, 420, 750, 920
MspR9I CCNGG 1 cut(s) 1064
MunI CAATTG 1 cut(s) 373
Mva1269I GAATGC 1 cut(s) 34
MvaI CCWGG 1 cut(s) 1064
MvnI CGCG 3 cut(s) 68, 891, 969
MwoI GCNNNNNNNGC 9 cut(s) 38, 100, 146, 153, 309, 416, 425, 530, 973
NarI GGCGCC 1 cut(s) 201
NdeII GATC 5 cut(s) 496, 752, 883, 916, 1240
NlaIII CATG 6 cut(s) 118, 863, 930, 976, 1038, 1074
NlaIV GGNNCC 6 cut(s) 202, 380, 754, 870, 902, 918
NmeAIII GCCGAG 3 cut(s) 123, 812, 986
NmuCI GTSAC 4 cut(s) 43, 581, 1127, 1184
NsiI ATGCAT 1 cut(s) 796
NspI RCATGY 2 cut(s) 930, 976
PaeI GCATGC 1 cut(s) 930
PauI GCGCGC 1 cut(s) 66
PciSI GCTCTTC 1 cut(s) 14
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 1 cut(s) 34
PdmI GAANNNNTTC 1 cut(s) 292
PfeI GAWTC 7 cut(s) 334, 391, 642, 673, 730, 801, 1085
PflFI GACNNNGTC 1 cut(s) 704
PflMI CCANNNNNTGG 1 cut(s) 106
PkrI GCNGC 5 cut(s) 57, 93, 96, 568, 925
PleI GAGTC 2 cut(s) 268, 1066
PluTI GGCGCC 1 cut(s) 204
PpsI GAGTC 2 cut(s) 268, 1066
Ppu21I YACGTR 1 cut(s) 527
PshAI GACNNNNGTC 1 cut(s) 122
Psp1406I AACGTT 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 1062
PspFI CCCAGC 1 cut(s) 554
PspGI CCWGG 1 cut(s) 1062
PspN4I GGNNCC 6 cut(s) 202, 380, 754, 870, 902, 918
PstNI CAGNNNCTG 1 cut(s) 1121
PsuI RGATCY 3 cut(s) 752, 916, 1240
PsyI GACNNNGTC 1 cut(s) 704
PteI GCGCGC 1 cut(s) 66
PvuII CAGCTG 1 cut(s) 881
RsaI GTAC 3 cut(s) 603, 1004, 1025
RsaNI GTAC 3 cut(s) 602, 1003, 1024
SapI GCTCTTC 1 cut(s) 14
SaqAI TTAA 2 cut(s) 357, 1249
SatI GCNGC 5 cut(s) 56, 92, 95, 567, 924
Sau3AI GATC 5 cut(s) 496, 752, 883, 916, 1240
ScaI AGTACT 1 cut(s) 1004
SchI GAGTC 2 cut(s) 269, 1067
ScrFI CCNGG 1 cut(s) 1064
SfaNI GCATC 1 cut(s) 625
SfcI CTRYAG 2 cut(s) 713, 1122
SfoI GGCGCC 1 cut(s) 202
SmlI CTYRAG 1 cut(s) 628
SmoI CTYRAG 1 cut(s) 628
SphI GCATGC 1 cut(s) 930
Sse9I AATT 2 cut(s) 373, 1246
SsiI CCGC 5 cut(s) 32, 95, 138, 812, 891
SspDI GGCGCC 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 1062
StyI CCWWGG 1 cut(s) 71
TaaI ACNGT 6 cut(s) 124, 228, 383, 789, 905, 1172
TaiI ACGT 4 cut(s) 50, 65, 452, 529
TaqI TCGA 1 cut(s) 394
TasI AATT 2 cut(s) 373, 1246
TatI WGTACW 2 cut(s) 1002, 1023
TauI GCSGC 1 cut(s) 97
TfiI GAWTC 7 cut(s) 334, 391, 642, 673, 730, 801, 1085
Tru1I TTAA 2 cut(s) 357, 1249
Tru9I TTAA 2 cut(s) 357, 1249
TscAI CASTG 4 cut(s) 435, 622, 902, 1052
TseFI GTSAC 4 cut(s) 43, 581, 1127, 1184
TseI GCWGC 4 cut(s) 55, 91, 566, 923
Tsp45I GTSAC 4 cut(s) 43, 581, 1127, 1184
TspDTI ATGAA 5 cut(s) 17, 402, 669, 793, 811
TspRI CASTG 4 cut(s) 435, 622, 902, 1052
Tth111I GACNNNGTC 1 cut(s) 704
Van91I CCANNNNNTGG 1 cut(s) 106
XceI RCATGY 2 cut(s) 930, 976
XcmI CCANNNNNNNNNTGG 2 cut(s) 857, 894
XmnI GAANNNNTTC 1 cut(s) 292
ZrmI AGTACT 1 cut(s) 1004
Zsp2I ATGCAT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.