Rmu_sc0003323.1_g000004

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003323.1
OG0007872
Physical Location & Seq
Reverse (-)
27874 .. 47827
19954 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 12525 bp
atgagaggttctgtgattttcgccattgttgttgaggcagtggagctcgctcttcagcttcaactcttcactcaaatcgagcaattgacagtgtgtgggagaagccaaaggtaccaagtaccagatgccaagtctttttcttggtctaattgtgctcaattgctatctgggttggttttgcttttttggtttatgctagtctctgctcttcgatcaattattgatattccggcgattgatcggttcgcaattcaaagccgttctgattcgggcaatgctttcagttccaacatgaagttgaagagaaggagggccgtggaagtgcctccgaaaattagatccttcatcaacagcgtcactgctgttccacttgagaatatagaggaacctttaaaaggttttgtctgggagtacgataagggagatttccatcattgggttgatctgttcaaccattttgattcattttttgagaagcatataaaatctaggaaggacttgcaggttgaggataattttctcgactctgatcctccttttccaagagaagctcttcttcaagttctccgcgtgataagaataattttggagaactgcacaaataaacacttctacagttcatatgagcagcatctctcatctttgcttgcttgcaccgatgcggatgtggttgaggcttgcctgcagactctggccgcatttttgaagaaaacagtaggaaagtattccatcagagatgcttcactaaattcaaagttgtttgctcttgcacaaggttggggaggaaaggaagagggtcttggattaattgcctgtgcagtacaggatggttgtgaccctattgcttatgagttaggatgcacccttcactttgagttttatgcgttgaaagatgcaagtgaattaccaaccacagaacagcagacccaaggtttacaaatcattcatttacctaacatcaacactcatccagagactgatcttgagcttttgagtaagctaattgccgaatacaaagtacccagtagtttgagatttgctttattgacaagattgcgttttgcaagggcttttggctctttagctactcgtcaacagtatgcctgcattcgtctgtatgccttcatagttcttgttcaagcaaatagtgatggcgatgaccttgtttctttcttcaacactgaaccggagtttgtcaatgaactagtgtcattattgagcttggaagatgtagttcctgagaaaattcgaattctatgtcttctttcattggttgctctttctcaagatcgggcccgacagcctaatgttttgactgctgtcacatctggtgggcatcgtggaatcctatccagcctcatgcagaaggccattgattccgtcatcagtgatacatcaaagtggtctgttgtttttgctgaggctctgttatctcttgttacagttttggtttcatcatcatccggttgttcagccatgcgtgaagctggatttatccccacccttctccctttgcttaaagatacaaatccacagcacctacatttggtcagcacatctgtgcatattttagaagcttttatggattacagtaatccagctgctgcattgttcagggacttgggcggtttagatgataccatctctcgcctgcagctggaagtgtctcatgtagaaaatgggccaaaacaacaagatgaagattctagtacctctggaagtgcacaagtagttgcaggaacttccactgagatagacagcatgcaacctctgtattcagaacctcttgtttcatatcatcgacgacttctgatgaaagctctactgcgtgctatatctctggggacttatgcacctggaaatactgcccgtgtttatggctcggaggagagcctattgccgcaatgcttatgcataatctttaaaagggcaaaagattttggtggtggggtgttttcacttgcggcttctgtcatgagtgatttaattcacaaagatcctacctgttttccagttttagatgaagcaggtcttccttctacctttctggatgctataatggatggcgttctttgctcaacagaggccattacatgcatcccacagtgtctggatgccctgtgtctaaataataatggtcttcaggctgtaaaagatcgcaatgcattgaggtgctttgtgaagatatttacttcaagaacctatctgcgtgctctaacaagtgatacacctgtctccctatctagtggattggatgaattaatgcgccatgcctcctcattacgtggtcctggtgtggatatgctaattgagattttaaatgccatctcaaaaattggacatgggggtgattcttctctcatgtcaaccgaacttttatcttcttctactcctgttcccatggaaactgatggagaagaaaggaatgtagtcatgtcagatgatagggagtcatccaagatggatagttcggagcagggaactgaaacatcttctgattctttagtgggaaatgctgaagtattgcttcctgattgtgtgagcaatgttgcacgtcttcttgaaactatacttcagaatggtgacacttgtcgtatatttgtggagaagaagggaatagaagctgtcctgcagttgtttaccttgcctttaatgcctctttctgtttcagttggtcagagtatatctattgcatttaaaaacttctcaccacaacattctgcttctttggctcgggcggtatgttcatttttgagagagcatctgaaatcaacaaatgaactcttagtttcagttggtgggactcaactttctttggtggaatctgcaaagcaaactaaggttttgaagcatctttccagtcttgaagctattctgtctctgtctaatgttctattgaaagggacggctactgtggtgtctgaactgggtgcagcagatgctgatgtattgaaagatcttgggagtacatacaaggaaatcctttggcaaatctctctgtgtaatgatgtcaagtctgatgagaagatcaatgctgaacaagaacaagacattgctgaggcagctccatctaatgcttcgggaagggagagtgatgatgatgctaatactcccatggtgagatacatgaacccagtttctattaggaatcagcccttttggggtggagagcgtgagttcctttcagttgttcgcagtggtgaaggtttgcaccgtcgtagtactcggcatgggattacacgcatgagaggaggaaggacaggccggcatttggaggctttacatattgattctgaatcttcttccacagtatcggaggcaactacatcccaggatttgaaaaagaaaagtcctgatgtgcttgttacagaaattcttaacaagctcgcttccactctacgttccttcttcacagctcttgtcaagggattcacatcaccaaataggcgtagagttgactctggatcattgagtttggcctcaaaaactcttggaactgccctagctaaagtttatcttgaggctcttggtttccctaggcattctacttcagctgggcttgatacttcattatcagtcaagtgtcgataccttggaaaagttgtggatgatatgatggcacttacatttgatagcagacgacgtacttgttatactgcaacaattaataatttctatgtccatggtacctttaaggagctgctcacaacgtttgaagctactagtcagttgttatggaccctaccatattgtatgccaacatcaggcattgatcatgaaaagacaggtgaaggcagtaaactgtcccatagttcgtggctgcttgagactctacaaagctattgccgtgtgctggagtattttgttaattcttctttactcttatctacaacctctgcatcccaggcacagttgcttgttcagccagttgcagttggtctatcaattgggcttttccctgtgccaagggaaccagaagtttttgttcgtatgcttcagtctcaagttcttgatgtaatacttcctatctggaaccaccccatgtttcctaattgtagtccaggattcgttgcttccattgtttcacttgttatgcatgtatattctggtgtgggagatgtgaagcagaatcgcagtggcattgctggaactgcaaaccaaagattcatgcctccaccacttgatgaagctactattactaccattatggagatgggtttttcaaggggcagagcagaagaagcgcttaggagggtggaaacaaatagcgttgaaatggccatggagtggctttgcagccatgctgaggatcctgtgcaggaggatgatgaactggcccgggcgcttgctctatcactgggaaattcatctgaaacatctaaagctgataatgttgacaagtctgtagatgtgctggcagaagagagttgtgtgaaagctcctccagttgatgatattcttgctgcatcagttaagttgtttcagagcagtgacacaatggccttcccattgacagatttgcttgtgacacttagcaatcggaacaaaggtgaagaccgtcctaaagttgcatcttatctaattcagcagctcaagctttgtccattggatttttctaaggataccagttctttgagtatgttatcacatgttatagcattgctgctttctgaggatggaagtacaagggaaattgctgcacagaatggtattgtgtcagctgcagtagatatcttaatgaattttaaggccaaagacgagtcaggcaatgaacttcttgttccaaaatgtattagtgctctactgcttattttggataacatgttgcaatcaaggcccagaatttctgagaacattgaagagactcagacaggatctctgtctgaactatctggagagcgtacttccttgtcaatccctgatgcagttacagagaaaaaacaggatacggatgccgaggaaaaagatagtggcactggatttgagaaaatattgggcaaatcaactggttatttgactatggaagagagtcaaaaggttctggccgttgcctgtgatttgataaagcagcatgtcccagctatgatcatgcaggctgttttacagctgtgtgctcgtttgacgaaaactcatgctcttgctttgcagtttcttgaacatggaggattagcggctctttttggccttcccagaagttgttttttccctggatatgacactgttgcatctgctattgttcgccatctccttgaagatccgcaaacactgcaaacagcaatggaattggaaattcgccaaacactgagtggcaacaggcatggtgcacgtacttctgcaaggacatttttgacatcaatggcacctgtaatctctagagatccggttgtttttatgaaagctgctgctgctgtttgtcagttggagacatcagcaggtaggacattcattgtcttaatgaaggaaaaagaaaaggaaaaggacaaacctaaagcatctggtggtgaggttggattatcttccaatgaatctatccgtattcctgaaaataaagtacatgatggatcaggtaaatgcttgaaaggtcacaagaagatccctgctaatcttactcaagtgattgatcagctgcttgaaattgttttgaaatactattttccaaagagccaggaagattgtcagaataacctgtcttctatggaggtagatgaacctgctaccaaggtaaagggtaaatcaaaggttgatgagacaaggaaagtggagtctgaatctgaaagatctgcagggctggcgaaggtaacatttgttctcaagttactgagtgatattcttctcatgtatgtacatgcagttggtgttatacttaaacgggatttggaattggctcaattacgggtagctaatcaactggaaggtcctggacatggtggtatacttcatcatgtgattcatcgattgcttccactcaccattgataaatctgctggacctgatgaatggagagacaagttatctgaaaaagcttcttggttcctggtagttctgtgtggtcgttctggtgaagggcgtagaagagtaattaatgagcttgtgaaagcattatcatcattttcaaacatcgatggtaattctttgaagagcatcttattgccagataaaaaggtttatgcttttgtggatttggtgtactctattttgtctaaaaattcatcatcaagcaacttacctggtacggggttttcacctgacatagcaaaaagcatgatagatgggggaatgattcagtgtctgactagtattctccaggtgattgatttggaccatcctgatgctcccaaaactgtgaatctcatactcaaggctttagaaagtctaaccagggctgctaatgctagtgagcaatattttaaatctgatgagacaaacaagaaaaagtcctcggtgttaaatagaggatctgatgatcaggtaactactccagctgatgatacattggggcataatcagaatacaagcagtgagcaggatgtcagagatgcagtaccaactgaacaacaggatcaaggaacttctcaaagtgaaggtaatccagatgcaactccgaaccagtcaggggaacatgatatgagaattgaagttgaaggaccattggcttctaaccaacccatggagcttgggatggattttatgcgtgaagagatggaagaaggcaatgtgttacacaacaatgaccaaattgaaatggcttttcgtgttgaaggtagggcagatgatgacatgggtgatgaagatgatgacatgggtgatgatggtgaggatgatgaggatgatgatgatggagaggatgaggatgaggatatagcagaagatggtggcggcatgatgtctttggctgatactgatgtggaagaccatgatgatactggcctgggagatgattataatgatgagatgattgatgaagatgatgatgatttccatgagaatcgtgtcatagaagtgagatggagggaagcccttgacgggcttgatcatttacacgtacttgggcaacctggagctgcaagtggtctgattgatgttgctgccgaaccttttgaaggggttaatgtggatgacctttttggtctacgaaggccgttaggttttgatcgacgtcgtcagacgagtaggtcttcctttgagcgatctgttacagaagcaaatggctttcaacatcctctccttataaggcctccccagtctggagatttggtatccatgtggtcagcaggtggaaattcatcccgggacttagaagctctatcatcagggagctttgatgttgctcatttctacatgtttgatgctcctgttcttccatatgatcatgttccaagcaatcttttcggtgaccgtttgggtggtgcagccccgccaccattgactgattattctgtaggtatggattcattgcagttatcgggacgaagaggcccaggtgatggccggtggactgatgatggccagcctcaagcaggtgctcatgctgctgctattgctcaggcagttgaggaacagtttatatcccaattgcgcagtcttgctcctgttgacacccctgttgaaccacattctcagaattcaggagtgcaggagaaacagccagatgtgcctccttcaactgatggtcaagtagaagtagatcgtggtaacaccaccagccaacaagttgaagatcaccaccaagacaggggtgttgaggtgacacatcaagatatctccaggcctgagggaattcctcaccaagaacaagtcaatcaagaatcctttgtcgaagatgcagggggctgtttgcaggaacctgaaccaatgtcaatccaggcaccttctttggacagtacacgaaatgataacatggacattgcggagggcaatggcgctgctgagcaagaagggtcaatgccggaatttgtcaactcatctgcaagcacaagggttgatttacaacaggaagagggttctgaagtgccttctaatgtcaatgatgctacagtagaagctatgggtcaggatggatcttcaggcaatcaagttggtgacatgccagcaaatttcggcttcaatgtgtctaattcagatgagccgtcaagccaaaacacattggttgctccagaagctaatcaggctgaacaagttactttgaacaatgaggctcctggtgctaatgcaattgaccctacattcttggaggctttacctgaagagttgagagcggaggttcttgcttcacagcaagctcagtcagttcaacctcctccttatgcgccaccttctgcggacgacattgatcctgagtttttagctgcccttcctccggatattcaagcagaagttttggctcaacaaagagcacaaagagttgcacaacaggctgaaggacaacctgttgacatggacaatgcttcaataattgctacttttcctgctgatttacgggaagaggtacttttaacttcttcagaagcagttttgtctgcattgccttctccactactggctgaagctcaaatgctaagagatcgagcaatgagtcattatcaggctcgcagccttttcgggagcagccacaggctgaataataggagaaatggattgggttttgaccggcatacagtaatggacaggggtgttggagtcacaattggccggagggcagtttcttctattacagatagcttaaaggtcaaggaaattgaaggcgagccacttctagatgcaaattcattgaaagccttaattaggcttctacgattagcacagcaagctcagtcagttcaacctcctccttatgcgccaccttctgcggacgacattgatcctgagtttttagccgcccttcctccggatattcaagcagaagttttggctcaacaaagagcacaaagagttgcacaacaggctgaaggacaacctgttgacatggacaatgcttcaataattgctacttttcctgctgatttacgggaagaggtacttttaacttcttcagaagcagttttgtctgcattgccttctccactactggctgaagctcaaatgctaagagatcgagcaatgagtcattatcaggctcgcagccttttcgggagcagccacaggctgaataataggagaaatggattgggttttgaccggcatacagtaatggacaggggtgttggagtcacaattggccggagggcagtttcttctattacagatagcttaaaggtcaaggaaattgaaggcgagccacttctagatgcaaattcattgaaagccttaattaggcttctacgattagcacagccccttggtaaaggactcttgcagaggctcttcttaatcctttgtacacacagtgttacaagagcaattttagtgcgtcttttgctcgatatgatcaagcctgaggctgaaggttcagtcagtggattagctactattaattcccaaaggctttatggttgccactcaaatgttgtatatggcagatctcagttgttagatggtcttcctcccttggtgttgcgtcggatccttgagatcttgacttatttagctacaaatcattctgcagtggcaaatatgttattctactttgatttctcaggtgttcctcagtctttaagtcccgtaaatatggataccaagaaggacaaaggcaaagagaaaatcggagaaggaggattttcatctaatcctgtcaacacccaggatggggatgttcccctgatattgtttctgaagctccttaatcgacctcatttcttgcgtagcactgctcatcttgagcaggtcatggacctgcttcaggttgttgttgataattcagcttcaaaattagaggtccattccccctctgaaagggtcgatgggaattcccaaaatctatctataagtgaaacttctggagatggtcagaatggtcgtcctgtggaaccagaatctcagcaagaggttaaacctgatggtgttggatcatcaacttcagatgcaaacaaaagtacagacacccatggtattttcttgaagctgccagaatccgatttgcataatctttgcagtcttcttggtcgtgaggggctttcagataaagtttatatgctttccagtgaggtgctgaagaagttggcctcagttgcagtgacccatcgaaagttctttatctcagagctttcagaattagctcatggtttgagtgcctccgctgtcagcgagcttgtcactctccggaatacacagatgttgggtttgagtgctggttctatggctgggtcggcaatcttacgagtattgcaatcactttgctcactcacttcacctggtacaaatgagaattcagttttggagaatgatgcagagcaggaggagcacgccaccatgtggaagctaaatattgcacttgagccattgtggcaagaattgagtgactgtataagtgccactgaaacacagttgggccagagctctttttgtccaaccatgtccactataaatattggggaccatgtacaaggttcctcttcttcatctcctcttcctcctggaacccagagactgcttccttttatggaggcattttttgtgttgtgtcaaaagctacaagcaaaccactctattacactgcaagatcaagctaatgtgactgcaagagaagtcaaagagtctgctggaaattcagatccttcaggtactaagttttatagctgtggggattctcagaggaagcttgatggtgctgtcacatttacaaggtttgcggagaagcatcgccgtcttttgaatgcatttatcagacagaatccagggctgttggagaaatcccttactatgatgttgaaggcaccacggctaattgattttgataacaagagagcatatttccggtcaagaattaggcaacagcatgagcaacatctctccggtccgctgcgaataagtgttcgtcgtgcttatgttttggaagattcttacaatcagttgagaatgcgtcctaatcaggacatgaagggacgattaaatgtccaattccaaggggaagagggtattgatgctggtggtctaacgcgagaatggtatcaattactatcaagggttatatttgacaaaggtgcattgcttttcactactgttggaaacaatgcaactttccagccaaaccctaattctgtataccagacggaacatctttcttactttaaatttgtgggccgcgtggttgcaaaggcagtatttgatgggcaacttttggatgtttatttcactcgatccttctacaagcacatacttggtgtaaaggtgacataccatgatatagaagccgtggatcctgattactacaagaatttgaaatggatgttagaggtgagtgggattctgcgtgcttatgttttggaagattcttacaatcagttgagaatgcgtcctaatcaggacatgaagggacgattaaatgtccaattccaaggggaagagggtattgatgctggtggtctaacgcgagaatggtatcaattactatcaagggttatatttgacaaaggtgcattgcttttcactactgtcggaaacaatgcaactttccagccgaaccctaattctgtataccagacggaacatctttcttactttaaatttgtgggccgcgtggttgcaaaggcagtatttgatgggcaacttttggatgtttatttcactcgatccttctacaagcacatacttggtgtaaaggtgacataccatgatatagaagctgtggatcctgattactacaagaatttgaaatggatgttagagaatgatgtgagtgatattcctgacctgacgtttagcatggatgcggatgaagaaaagcatattctttatgagaaaaaccaggtgactgattatgagcttaaacctggaggacgaaatatcagagttacggaagaaactaagcacgagtatgtagaccttgtagctgatcatattttgacaaatgctattcgtccacaaattaattctttcttggagggcttcaatgaattggttccacgtgagcttatctcgatttttaatgataaagagcttgagctacttatcagtggtcttccggagattgatttggatgacttgaaggcaaacaccgagtacactggctatacagcagcatctagtgttgtacaatggttttgggaggtggttaaatgtttcaacaaggaggacatggcaagactgcttcaatttgtaactggcacatcaaaggttccattggaaggtttcaaggctttgcaaggcatctctggtcctcagaggtttcagattcacaaggcatatggagctcctgacaggctgccctctgctcatacatgctttaatcaactagaccttcctgagtatacctctaaggatcagcttcatgaacgtcatctgtctcaagtaccatgtacatatgaaagagccggtactgatttagagctttcagaattacaatcaccaaggatacgaggatggttttggtga

Protein Analysis

4174

Amino Acids

458.99

Weight (kDa)

5.02

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF908 PF06012 182 - 258 4e-12 Domain of Unknown Function (DUF908)
DUF908 PF06012 303 - 458 2.4e-26 Domain of Unknown Function (DUF908)
DUF913 PF06025 519 - 887 8e-92 Domain of Unknown Function (DUF913)
UBA_7 PF22562 1395 - 1439 2.3e-13 UBA-like domain
UBA PF00627 1395 - 1431 1.6e-06 UBA/TS-N domain
UBM PF14377 2734 - 2761 2.3e-08 Ubiquitin binding region
UBM PF14377 2771 - 2800 1.8e-10 Ubiquitin binding region
UBM PF14377 2807 - 2838 2.3e-07 Ubiquitin binding region
UBM PF14377 2947 - 2976 1.8e-10 Ubiquitin binding region
UBM PF14377 2983 - 3014 2.3e-07 Ubiquitin binding region
HECT PF00632 3703 - 3797 4.9e-19 HECT-domain (ubiquitin-transferase)
HECT PF00632 3847 - 4143 7e-90 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 6942, 7229
AarI CACCTGC 2 cut(s) 7262, 7508
AasI GACNNNNNNGTC 1 cut(s) 7171
AatII GACGTC 1 cut(s) 7159
Acc16I TGCGCA 1 cut(s) 7577
Acc36I ACCTGC 8 cut(s) 493, 2018, 5423, 5719, 7262, 7508, 9808, 9837
Acc65I GGTACC 2 cut(s) 111, 3686
AccB1I GGYRCC 5 cut(s) 111, 3686, 5359, 7864, 10867
AccB7I CCANNNNNTGG 4 cut(s) 4287, 6667, 7484, 10407
AccBSI CCGCTC 1 cut(s) 8255
AccI GTMKAC 6 cut(s) 5932, 7129, 11196, 11628, 11973, 12401
AccII CGCG 5 cut(s) 570, 11092, 11238, 11524, 11670
AccIII TCCGGA 4 cut(s) 8356, 8884, 10254, 12115
AclI AACGTT 1 cut(s) 3710
AcoI YGGCCR 7 cut(s) 693, 4278, 5043, 7486, 7504, 8686, 9214
AcvI CACGTG 1 cut(s) 12059
AcyI GRCGYC 1 cut(s) 7156
AdeI CACNNNGTG 3 cut(s) 5306, 9383, 10407
AfeI AGCGCT 1 cut(s) 4245
AflIII ACRYGT 4 cut(s) 4648, 4821, 7039, 7338
AhlI ACTAGT 3 cut(s) 1204, 3722, 6293
AjiI CACGTC 1 cut(s) 2555
AjuI GAANNNNNNNTTGG 6 cut(s) 783, 815, 10352, 10384, 11173, 11205
AleI CACNNNNGTG 1 cut(s) 1559
Alw44I GTGCAC 2 cut(s) 1722, 5322
AlwNI CAGNNNCTG 7 cut(s) 691, 977, 1604, 2110, 2942, 6289, 10582
Ama87I CYCGRG 3 cut(s) 2733, 4338, 7287
Aor13HI TCCGGA 4 cut(s) 8356, 8884, 10254, 12115
Aor51HI AGCGCT 1 cut(s) 4245
ApaI GGGCCC 1 cut(s) 1297
ApaLI GTGCAC 2 cut(s) 1722, 5322
ArsI GACNNNNNNTTYG 2 cut(s) 1243, 1275
AseI ATTAAT 6 cut(s) 806, 2261, 3666, 6081, 9468, 12021
Asp700I GAANNNNTTC 4 cut(s) 552, 724, 2871, 12051
Asp718I GGTACC 2 cut(s) 111, 3686
AspA2I CCTAGG 1 cut(s) 3536
AspLEI GCGC 7 cut(s) 2268, 4246, 4345, 7578, 7922, 8308, 8836
AsuC2I CCSGG 4 cut(s) 4339, 4340, 7288, 7289
AsuII TTCGAA 1 cut(s) 1249
AvaI CYCGRG 3 cut(s) 2733, 4338, 7287
AvrII CCTAGG 1 cut(s) 3536
AxyI CCTNAGG 2 cut(s) 7770, 9432
BaeGI GKGCMC 3 cut(s) 1297, 1726, 5326
BaeI ACNNNNGTAYC 4 cut(s) 2217, 2250, 6888, 6921
BalI TGGCCA 2 cut(s) 4280, 7506
BamHI GGATCC 4 cut(s) 4309, 9558, 11350, 11782
BanI GGYRCC 5 cut(s) 111, 3686, 5359, 7864, 10867
BanII GRGCYC 4 cut(s) 48, 1297, 10493, 12346
BauI CACGAG 1 cut(s) 11963
BbrPI CACGTG 1 cut(s) 12059
BbvCI CCTCAGC 3 cut(s) 1419, 3057, 4305
BcgI CGANNNNNNTGC 6 cut(s) 7369, 7403, 8578, 8612, 9106, 9140
BciVI GTATCC 5 cut(s) 4615, 4939, 7267, 9661, 12498
BclI TGATCA 8 cut(s) 3772, 5085, 5620, 6463, 7030, 7366, 9423, 11986
BcnI CCSGG 4 cut(s) 4339, 4340, 7288, 7289
BcuI ACTAGT 3 cut(s) 1204, 3722, 6293
BfoI RGCGCY 3 cut(s) 4247, 4346, 7923
BfuAI ACCTGC 8 cut(s) 493, 2018, 5423, 5719, 7262, 7508, 9808, 9837
BfuI GTATCC 5 cut(s) 4615, 4939, 7267, 9661, 12498
BglI GCCNNNNNGGC 1 cut(s) 10438
BglII AGATCT 4 cut(s) 2956, 5777, 9515, 9567
BlnI CCTAGG 1 cut(s) 3536
BlpI GCTNAGC 1 cut(s) 7926
BmcAI AGTACT 1 cut(s) 3223
BmeT110I CYCGRG 3 cut(s) 2733, 4338, 7287
BmgBI CACGTC 1 cut(s) 2555
BmrI ACTGGG 5 cut(s) 1017, 2936, 3128, 4367, 7234
BmuI ACTGGG 5 cut(s) 1017, 2936, 3128, 4367, 7234
BoxI GACNNNNGTC 2 cut(s) 1319, 2589
BplI GAGNNNNNCTC 4 cut(s) 12053, 12085, 12389, 12421
Bpu10I CCTNAGC 5 cut(s) 1419, 3057, 4247, 4305, 7542
Bpu1102I GCTNAGC 1 cut(s) 7926
Bpu14I TTCGAA 1 cut(s) 1249
BpuMI CCSGG 4 cut(s) 4339, 4340, 7288, 7289
Bsa29I ATCGAT 2 cut(s) 5953, 6120
BsaAI YACGTR 4 cut(s) 2285, 5327, 7042, 12059
BsaBI GATNNNNATC 4 cut(s) 429, 1527, 3433, 9715
BsaHI GRCGYC 1 cut(s) 7156
BsaWI WCCGGW 9 cut(s) 1186, 1463, 5380, 8356, 8884, 10254, 10908, 10946, 12115
BsaXI ACNNNNNCTCC 4 cut(s) 7206, 7236, 7239, 7269
Bse118I RCCGGY 5 cut(s) 3264, 7488, 8646, 9174, 12464
Bse21I CCTNAGG 2 cut(s) 7770, 9432
Bse8I GATNNNNATC 4 cut(s) 429, 1527, 3433, 9715
BseAI TCCGGA 4 cut(s) 8356, 8884, 10254, 12115
BseCI ATCGAT 2 cut(s) 5953, 6120
BseJI GATNNNNATC 4 cut(s) 429, 1527, 3433, 9715
BseRI GAGGAG 8 cut(s) 1901, 2266, 3264, 4431, 8286, 8814, 10406, 10548
BseSI GKGCMC 3 cut(s) 1297, 1726, 5326
BseYI CCCAGC 3 cut(s) 3554, 5077, 10295
BsgI GTGCAG 7 cut(s) 580, 837, 2952, 4337, 4683, 7428, 7652
Bsh1236I CGCG 5 cut(s) 570, 11092, 11238, 11524, 11670
BshNI GGYRCC 5 cut(s) 111, 3686, 5359, 7864, 10867
BshVI ATCGAT 2 cut(s) 5953, 6120
BsiHKCI CYCGRG 3 cut(s) 2733, 4338, 7287
BsmI GAATGC 5 cut(s) 1107, 3541, 10813, 11016, 11448
BsoBI CYCGRG 3 cut(s) 2733, 4338, 7287
Bsp119I TTCGAA 1 cut(s) 1249
Bsp120I GGGCCC 1 cut(s) 1293
Bsp13I TCCGGA 4 cut(s) 8356, 8884, 10254, 12115
Bsp1407I TGTACA 5 cut(s) 5842, 9374, 10534, 12184, 12449
Bsp1720I GCTNAGC 1 cut(s) 7926
Bsp19I CCATGG 6 cut(s) 2400, 3114, 3682, 4281, 6666, 10039
BspDI ATCGAT 2 cut(s) 5953, 6120
BspEI TCCGGA 4 cut(s) 8356, 8884, 10254, 12115
BspFNI CGCG 5 cut(s) 570, 11092, 11238, 11524, 11670
BspHI TCATGA 3 cut(s) 1974, 3775, 12421
BspMAI CTGCAG 6 cut(s) 687, 1656, 2634, 4726, 5785, 9601
BspMI ACCTGC 8 cut(s) 493, 2018, 5423, 5719, 7262, 7508, 9808, 9837
BspQI GCTCTTC 5 cut(s) 57, 213, 558, 6131, 9365
BspT104I TTCGAA 1 cut(s) 1249
BspT107I GGYRCC 5 cut(s) 111, 3686, 5359, 7864, 10867
BsrBI CCGCTC 1 cut(s) 8255
BsrFI RCCGGY 5 cut(s) 3264, 7488, 8646, 9174, 12464
BsrGI TGTACA 5 cut(s) 5842, 9374, 10534, 12184, 12449
BssAI RCCGGY 5 cut(s) 3264, 7488, 8646, 9174, 12464
BssNAI GTATAC 4 cut(s) 5933, 11197, 11629, 12402
BssNI GRCGYC 1 cut(s) 7156
BssSI CACGAG 1 cut(s) 11963
Bst1107I GTATAC 4 cut(s) 5933, 11197, 11629, 12402
Bst2BI CACGAG 1 cut(s) 11963
BstACI GRCGYC 1 cut(s) 7156
BstAPI GCANNNNNTGC 2 cut(s) 2939, 5266
BstAUI TGTACA 5 cut(s) 5842, 9374, 10534, 12184, 12449
BstBAI YACGTR 4 cut(s) 2285, 5327, 7042, 12059
BstBI TTCGAA 1 cut(s) 1249
BstDSI CCRYGG 9 cut(s) 315, 2400, 3114, 3682, 4281, 6666, 10039, 10871, 11346
BstEII GGTNACC 1 cut(s) 7391
BstENI CCTNNNNNAGG 7 cut(s) 393, 7098, 7515, 8779, 9307, 9833, 9886
BstFNI CGCG 5 cut(s) 570, 11092, 11238, 11524, 11670
BstH2I RGCGCY 3 cut(s) 4247, 4346, 7923
BstHHI GCGC 7 cut(s) 2268, 4246, 4345, 7578, 7922, 8308, 8836
BstPAI GACNNNNGTC 2 cut(s) 1319, 2589
BstPI GGTNACC 1 cut(s) 7391
BstSLI GKGCMC 3 cut(s) 1297, 1726, 5326
BstUI CGCG 5 cut(s) 570, 11092, 11238, 11524, 11670
BstZ17I GTATAC 4 cut(s) 5933, 11197, 11629, 12402
Bsu15I ATCGAT 2 cut(s) 5953, 6120
Bsu36I CCTNAGG 2 cut(s) 7770, 9432
BsuI GTATCC 5 cut(s) 4615, 4939, 7267, 9661, 12498
BsuTUI ATCGAT 2 cut(s) 5953, 6120
BtgI CCRYGG 9 cut(s) 315, 2400, 3114, 3682, 4281, 6666, 10039, 10871, 11346
BtgZI GCGATG 2 cut(s) 1170, 10778
BtrI CACGTC 1 cut(s) 2555
BveI ACCTGC 8 cut(s) 493, 2018, 5423, 5719, 7262, 7508, 9808, 9837
CaiI CAGNNNCTG 7 cut(s) 691, 977, 1604, 2110, 2942, 6289, 10582
CciI TCATGA 3 cut(s) 1974, 3775, 12421
CfoI GCGC 7 cut(s) 2268, 4246, 4345, 7578, 7922, 8308, 8836
Cfr10I RCCGGY 5 cut(s) 3264, 7488, 8646, 9174, 12464
Cfr9I CCCGGG 2 cut(s) 4338, 7287
ClaI ATCGAT 2 cut(s) 5953, 6120
CpoI CGGWCCG 1 cut(s) 10949
CseI GACGC 5 cut(s) 343, 9395, 9542, 11003, 11435
CsiI ACCWGGT 3 cut(s) 6226, 10345, 11898
CspCI CAANNNNNGTGG 2 cut(s) 5739, 5774
CspI CGGWCCG 1 cut(s) 10949
DraI TTTAAA 7 cut(s) 393, 1924, 2319, 2698, 6409, 11224, 11656
DraIII CACNNNGTG 3 cut(s) 5306, 9383, 10407
DrdI GACNNNNNNGTC 1 cut(s) 7171
DseDI GACNNNNNNGTC 1 cut(s) 7171
EaeI YGGCCR 7 cut(s) 693, 4278, 5043, 7486, 7504, 8686, 9214
Ecl136II GAGCTC 3 cut(s) 46, 10491, 12344
Eco147I AGGCCT 2 cut(s) 7234, 7768
Eco24I GRGCYC 4 cut(s) 48, 1297, 10493, 12346
Eco32I GATATC 2 cut(s) 4732, 7759
Eco47III AGCGCT 1 cut(s) 4245
Eco53kI GAGCTC 3 cut(s) 46, 10491, 12344
Eco72I CACGTG 1 cut(s) 12059
Eco81I CCTNAGG 2 cut(s) 7770, 9432
Eco88I CYCGRG 3 cut(s) 2733, 4338, 7287
Eco91I GGTNACC 1 cut(s) 7391
EcoICRI GAGCTC 3 cut(s) 46, 10491, 12344
EcoNI CCTNNNNNAGG 7 cut(s) 393, 7098, 7515, 8779, 9307, 9833, 9886
EcoO109I RGGNCCY 1 cut(s) 5915
EcoO65I GGTNACC 1 cut(s) 7391
EcoRI GAATTC 5 cut(s) 1251, 7621, 7776, 9901, 10360
EcoRV GATATC 2 cut(s) 4732, 7759
EcoT22I ATGCAT 5 cut(s) 1916, 2099, 2167, 4098, 10813
EcoT38I GRGCYC 4 cut(s) 48, 1297, 10493, 12346
FauI CCCGC 1 cut(s) 7422
FauNDI CATATG 4 cut(s) 622, 7363, 12337, 12454
FbaI TGATCA 8 cut(s) 3772, 5085, 5620, 6463, 7030, 7366, 9423, 11986
FblI GTMKAC 6 cut(s) 5932, 7129, 11196, 11628, 11973, 12401
FriOI GRGCYC 4 cut(s) 48, 1297, 10493, 12346
FspI TGCGCA 1 cut(s) 7577
GlaI GCGC 7 cut(s) 2267, 4245, 4344, 7577, 7921, 8307, 8835
GsaI CCCAGC 3 cut(s) 3558, 5081, 10299
HaeII RGCGCY 3 cut(s) 4247, 4346, 7923
HgaI GACGC 5 cut(s) 343, 9395, 9542, 11003, 11435
HhaI GCGC 7 cut(s) 2268, 4246, 4345, 7578, 7922, 8308, 8836
Hin1I GRCGYC 1 cut(s) 7156
Hin6I GCGC 7 cut(s) 2266, 4244, 4343, 7576, 7920, 8306, 8834
HinP1I GCGC 7 cut(s) 2266, 4244, 4343, 7576, 7920, 8306, 8834
HincII GTYRAC 9 cut(s) 1094, 2367, 3457, 4396, 7594, 7957, 8431, 8959, 9730
HindII GTYRAC 9 cut(s) 1094, 2367, 3457, 4396, 7594, 7957, 8431, 8959, 9730
HindIII AAGCTT 4 cut(s) 1575, 4595, 6021, 10751
Hpy99I CGWCG 7 cut(s) 1806, 3219, 3645, 7158, 7161, 9558, 10974
Hsp92I GRCGYC 1 cut(s) 7156
HspAI GCGC 7 cut(s) 2266, 4244, 4343, 7576, 7920, 8306, 8834
Kpn2I TCCGGA 4 cut(s) 8356, 8884, 10254, 12115
KpnI GGTACC 2 cut(s) 115, 3690
KroI GCCGGC 1 cut(s) 3264
KroNI GCCGGC 1 cut(s) 3266
Ksp22I TGATCA 8 cut(s) 3772, 5085, 5620, 6463, 7030, 7366, 9423, 11986
LguI GCTCTTC 5 cut(s) 57, 213, 558, 6131, 9365
MabI ACCWGGT 3 cut(s) 6226, 10345, 11898
MbiI CCGCTC 1 cut(s) 8255
MfeI CAATTG 7 cut(s) 83, 158, 3945, 7571, 8211, 8682, 9210
MlsI TGGCCA 2 cut(s) 4280, 7506
MluNI TGGCCA 2 cut(s) 4280, 7506
Mox20I TGGCCA 2 cut(s) 4280, 7506
Mph1103I ATGCAT 5 cut(s) 1916, 2099, 2167, 4098, 10813
MroI TCCGGA 4 cut(s) 8356, 8884, 10254, 12115
MroNI GCCGGC 1 cut(s) 3264
MroXI GAANNNNTTC 4 cut(s) 552, 724, 2871, 12051
MscI TGGCCA 2 cut(s) 4280, 7506
MslI CAYNNNNRTG 9 cut(s) 1399, 1559, 2170, 2346, 6327, 6726, 6998, 9498, 11967
Msp20I TGGCCA 2 cut(s) 4280, 7506
MspA1I CMGCKG 9 cut(s) 1601, 1657, 3554, 4721, 5107, 5626, 6482, 10232, 10954
MunI CAATTG 7 cut(s) 83, 158, 3945, 7571, 8211, 8682, 9210
Mva1269I GAATGC 5 cut(s) 1107, 3541, 10813, 11016, 11448
MvnI CGCG 5 cut(s) 570, 11092, 11238, 11524, 11670
NaeI GCCGGC 1 cut(s) 3266
NciI CCSGG 4 cut(s) 4339, 4340, 7288, 7289
NcoI CCATGG 6 cut(s) 2400, 3114, 3682, 4281, 6666, 10039
NdeI CATATG 4 cut(s) 622, 7363, 12337, 12454
NgoMIV GCCGGC 1 cut(s) 3264
NmeAIII GCCGAG 2 cut(s) 3205, 4981
NsbI TGCGCA 1 cut(s) 7577
NsiI ATGCAT 5 cut(s) 1916, 2099, 2167, 4098, 10813
NspV TTCGAA 1 cut(s) 1249
OliI CACNNNNGTG 1 cut(s) 1559
PaeI GCATGC 1 cut(s) 1765
PagI TCATGA 3 cut(s) 1974, 3775, 12421
PaqCI CACCTGC 2 cut(s) 7262, 7508
PceI AGGCCT 2 cut(s) 7234, 7768
PciI ACATGT 3 cut(s) 4648, 4821, 7338
PciSI GCTCTTC 5 cut(s) 57, 213, 558, 6131, 9365
PctI GAATGC 5 cut(s) 1107, 3541, 10813, 11016, 11448
PdiI GCCGGC 1 cut(s) 3266
PdmI GAANNNNTTC 4 cut(s) 552, 724, 2871, 12051
PflFI GACNNNGTC 1 cut(s) 7158
PflMI CCANNNNNTGG 4 cut(s) 4287, 6667, 7484, 10407
PfoI TCCNGGA 3 cut(s) 4060, 5917, 10567
PmaCI CACGTG 1 cut(s) 12059
PmlI CACGTG 1 cut(s) 12059
Ppu21I YACGTR 4 cut(s) 2285, 5327, 7042, 12059
PpuMI RGGWCCY 1 cut(s) 5915
PscI ACATGT 3 cut(s) 4648, 4821, 7338
PshAI GACNNNNGTC 2 cut(s) 1319, 2589
PshBI ATTAAT 6 cut(s) 806, 2261, 3666, 6081, 9468, 12021
PsiI TTATAA 2 cut(s) 6942, 7229
Psp124BI GAGCTC 3 cut(s) 48, 10493, 12346
Psp1406I AACGTT 1 cut(s) 3710
Psp5II RGGWCCY 1 cut(s) 5915
PspCI CACGTG 1 cut(s) 12059
PspEI GGTNACC 1 cut(s) 7391
PspFI CCCAGC 3 cut(s) 3554, 5077, 10295
PspOMI GGGCCC 1 cut(s) 1293
PspPPI RGGWCCY 1 cut(s) 5915
PsrI GAACNNNNNNTAC 2 cut(s) 5790, 5822
PstI CTGCAG 6 cut(s) 687, 1656, 2634, 4726, 5785, 9601
PstNI CAGNNNCTG 7 cut(s) 691, 977, 1604, 2110, 2942, 6289, 10582
PsyI GACNNNGTC 1 cut(s) 7158
PvuII CAGCTG 7 cut(s) 1601, 1657, 3554, 4721, 5107, 5626, 6482
RseI CAYNNNNRTG 9 cut(s) 1399, 1559, 2170, 2346, 6327, 6726, 6998, 9498, 11967
Rsr2I CGGWCCG 1 cut(s) 10949
RsrII CGGWCCG 1 cut(s) 10949
SacI GAGCTC 3 cut(s) 48, 10493, 12346
SapI GCTCTTC 5 cut(s) 57, 213, 558, 6131, 9365
ScaI AGTACT 1 cut(s) 3223
SexAI ACCWGGT 3 cut(s) 6226, 10345, 11898
SfuI TTCGAA 1 cut(s) 1249
SmaI CCCGGG 2 cut(s) 4340, 7289
SmiMI CAYNNNNRTG 9 cut(s) 1399, 1559, 2170, 2346, 6327, 6726, 6998, 9498, 11967
SpeI ACTAGT 3 cut(s) 1204, 3722, 6293
SphI GCATGC 1 cut(s) 1765
SrfI GCCCGGGC 1 cut(s) 4340
SseBI AGGCCT 2 cut(s) 7234, 7768
SspI AATATT 4 cut(s) 4992, 6404, 10420, 10522
SstI GAGCTC 3 cut(s) 48, 10493, 12346
StuI AGGCCT 2 cut(s) 7234, 7768
TauI GCSGC 8 cut(s) 698, 1903, 1967, 5174, 6879, 8876, 11238, 11670
TspGWI ACGGA 6 cut(s) 1369, 4964, 5522, 11219, 11651, 11963
TspMI CCCGGG 2 cut(s) 4338, 7287
Tth111I GACNNNGTC 1 cut(s) 7158
Van91I CCANNNNNTGG 4 cut(s) 4287, 6667, 7484, 10407
VneI GTGCAC 2 cut(s) 1722, 5322
VspI ATTAAT 6 cut(s) 806, 2261, 3666, 6081, 9468, 12021
XagI CCTNNNNNAGG 7 cut(s) 393, 7098, 7515, 8779, 9307, 9833, 9886
XbaI TCTAGA 3 cut(s) 5372, 8752, 9280
XcmI CCANNNNNNNNNTGG 4 cut(s) 4210, 5303, 6920, 9482
XmaI CCCGGG 2 cut(s) 4338, 7287
XmaJI CCTAGG 1 cut(s) 3536
XmiI GTMKAC 6 cut(s) 5932, 7129, 11196, 11628, 11973, 12401
XmnI GAANNNNTTC 4 cut(s) 552, 724, 2871, 12051
ZraI GACGTC 1 cut(s) 7157
ZrmI AGTACT 1 cut(s) 3223
Zsp2I ATGCAT 5 cut(s) 1916, 2099, 2167, 4098, 10813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.