Rmu_sc0003610.1_g000027

ATP-dependent helicase hrq1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003610.1
OG0006845
Physical Location & Seq
Reverse (-)
107192 .. 115049
7858 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3639 bp
atggcagcgagtcggattcaaatcgaggtccgtacattgaccggcgaaacgaccaccgtctcagcctcggtcaacgacgccgtttcagacctcaagctacttctcaaccagtccttccctcccgccaccaactctcccaatttccacctcttcttcaagggtgagaagttgaggttgcagagtcgaataggcacacacgggattaagccgggtgaggttctagtgctggtcccgtttgttaaaaaggtggctaataatcagagccagaaatgtgatcaatctgatccatcgttgaatacttctgctaatcgctccatgtccaagttttcgaactccgcatggtctgatatgatgcaggacttgtcatatttgcgtgatggttcgagtgatggaacccccagtgtgtctaatatagggagttttagtgttggagatggaagtggagattgctcttgtgaagtgaagcggaagagggggtttggttgtgatgagatgatattggatatgttgtgggctagtagaagcaagagtgttcttgatgaacagaattctagaaggtttgttgagcttttggagtcggtgaattgtttatcggatccatattctggggactgcatgttgtggagaagaaaagctagcttacaaggctttggcttggggctgcctaacagcaatggtaattcatgcttatgtccagagtggttgaagaaaatcatgaaggggtttgctttcttaaacattttttctgcacttgttcaacttcagcaggagaggacaacctcaactctattggagcaagcactgaaacagcttgcgacgtttggagttaaacttgatatgcaggacatggagaatctttcggttatttctcccaaggtagttctttttgtgaagaagaatgcagaggagacaagttctggtaatgcactagttattattgaatgctcggcagaacagaaccatggagacagaaataatcatgaatctgcttggaaacagatggatgtatcaaagattgtcaatgaattgaagttgcaggaaagttctttcaaaagtaatttgtgggaggctgttgagaggttaatgtttatcaaaagaaatgagatttcctccttggaagactttctcatttctgtgaaggattgcggtgctgctgcaagaggaaataaagttaatcaagcaacgaaaagccacacagcttcaactactgattcagacaaaaagcactctgcagcttcaacctctcgttcacacaaaaagcgagcactatgtcatagaacaggtccattgctaccagttgagatggttgaacatcttagaaagagaattggatcagcaacgaagatagtgcatgtcgaagacattggtgctcgaaatgccgttcatgtggaagttccacaggaactatcagaaaatctgagatctgtacttcattctattggaatcactcagttatatagccatcaggcagagtcgatacaagcctcactaactggcaagaatgttgttgtggctacaatgacatccagtgggaaatctctttgctacaatctaccggtcattgaagccctgtcccagagttcctcatcatgtgctctatacttatttccaacgaaggctttagctcaagatcaactaagagctttcttagctatgaccaaaggattcagtggtagcttaaatgttggtatatatgatggtgacactaatcagcaggaaaggacacgactgcgtcaaaatgctagactgctaatcacaaatccagatatgttgcatgtatcaatcctgccacaccaccgaaaatttggccggattttggcaaatcttaggtatgtagtgattgatgaggcccatgtttataagggagcatttggttgtcacactgctcttatacttaggcgacttcgccggctctgttcacatgtgtatggaagtgatccttcttttgtgttttctactgcgacttcagcaaatcctcgtgagcattgcatggaacttgcgaatttaccaactctagagttgattcagaatgatggaagccctgctgctagaaagcttttcatcctttggaattctgaggacagtaatcttgacagcgcaattgaggatatatcattcctgtttgctgaaatggtccagcatggactacggtgcattgcattttgcaaaacacgcaagctttgtgaacttgttttttgccatacgcgtgaaattcttcaggagacagcaccccatctggcagagtctatatgttcttatcgtgctggttacattgctgaggataggagaagaatagagagtgatttatttaacgggaaactttctggtattgctgcaacaaatgcgcttgaattgggtattgatgtcggacacattgatgtaactcttcatttgggttttcccggaagtattgctagcttgtggcaacaagctggcagagccggaaggagagagagaccttctcttgctgtatatgttgcattcgaagggccccttgaccaatattttatgaaacatccaaataaactttttggaagcccaattgagtgctgtcatgttgatgctaagaaccaacaggttcttgaacagcatttggcttgtgctgctcttgaacacccactgagcttgttttatgatgggaagtttttcggttctggcttaaacagcgccataatgtctcttaaaagtagaggatttctgagttatgatccatcatgtgattctgctgctaaaatctggaactatattgggtatcagaaaatgccttcacattcagttagtattcgatcaattgaaagagagagatacaaagttatggacgatcaagagaaagaaatcgaagagattgaggaaagcaaggctttctttcaggtatatgaaggtgctgtttatttgaaccaaggaaagacatacctggtcacgagcttagatttatctagcaagattgcttggtgccaaaaagctgatgtaaattattatacgaaaccccgtgatcgcacagatattcatgtagttggtggtaatgtggcttatccagctccagctaagctttccaaccagttctccagaacaactgcccgagcagatccttgcagagtaacaactacatggtttggattctatcgtgtacacaaaggaagcaacatgatatttgataaagttaaccttgcacttcctaaatatacgtatacgtcacaggcagtttgggttccggttccacgatcaataattaaagcagtggagagtaaacaacttgaataccgtgcagggctgcatgctgcatcacatgctgttctgcacgttgtgcctctacgaatcatttgtaacttatctgacttggctccagagtgtgcggaccctcatgacggcagctattttccggagaggattctattatatgatcagcatcctggagggactggtgtctgtgtacaggttcatccattttttatggagctcttggctactgctctggaactcctgacatcttgccactgcagagaagatgatggttgccctaattgcattcagagtttttgttgcaagtcatataatgaggttttacacaaggatgcagcaattatgatcattaagggtattctggatgcgaatatatcttctgactcttctggatcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DUF1998 Helicase_C

Protein Analysis

1212

Amino Acids

134.51

Weight (kDa)

7.8

Isoelectric Point (pI)

47.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 484 - 621 4.9e-07 Type III restriction enzyme, res subunit
DEAD PF00270 487 - 657 1.1e-30 DEAD/DEAH box helicase
Helicase_C PF00271 711 - 817 1.6e-12 Helicase conserved C-terminal domain
WHD_HRQ1 PF22982 853 - 931 5.8e-16 ATP-dependent helicase HRQ1, winged helix domain
MZB PF09369 1094 - 1176 7.9e-18 MrfA Zn-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1860
AccB1I GGYRCC 1 cut(s) 2952
AccB7I CCANNNNNTGG 1 cut(s) 605
AccI GTMKAC 1 cut(s) 3188
AccII CGCG 1 cut(s) 2206
AccIII TCCGGA 1 cut(s) 3379
AciI CCGC 5 cut(s) 123, 336, 466, 1146, 3353
AclWI GGATC 7 cut(s) 278, 590, 603, 1339, 1931, 2702, 3080
AcoI YGGCCR 1 cut(s) 1807
AcsI RAATTY 5 cut(s) 547, 1802, 2002, 2071, 2211
AcuI CTGAAG 3 cut(s) 746, 1950, 2201
AcyI GRCGYC 1 cut(s) 78
AdeI CACNNNGTG 1 cut(s) 3304
AfaI GTAC 4 cut(s) 34, 1428, 3129, 3432
AfiI CCNNNNNNNGG 3 cut(s) 605, 1816, 3365
AflIII ACRYGT 2 cut(s) 1921, 2204
AgeI ACCGGT 1 cut(s) 1555
AhlI ACTAGT 1 cut(s) 928
AjnI CCWGG 2 cut(s) 2913, 3409
Alw21I GWGCWC 4 cut(s) 1267, 1372, 1597, 3459
Alw26I GTCTC 6 cut(s) 64, 902, 960, 2216, 2449, 2682
AlwI GGATC 7 cut(s) 278, 590, 603, 1339, 1931, 2702, 3080
Ama87I CYCGRG 1 cut(s) 3078
Aor13HI TCCGGA 1 cut(s) 3379
AoxI GGCC 3 cut(s) 1807, 1848, 2489
ApaI GGGCCC 1 cut(s) 2493
ApoI RAATTY 5 cut(s) 547, 1802, 2002, 2071, 2211
AsiGI ACCGGT 1 cut(s) 1555
Asp700I GAANNNNTTC 3 cut(s) 1122, 2214, 2645
AspLEI GCGC 3 cut(s) 2099, 2347, 2669
AspS9I GGNCC 8 cut(s) 28, 229, 1283, 1849, 2134, 2489, 2490, 3355
AsuC2I CCSGG 2 cut(s) 210, 2403
AsuHPI GGTGA 4 cut(s) 173, 224, 592, 1712
AsuII TTCGAA 2 cut(s) 329, 2484
AsuNHI GCTAGC 2 cut(s) 635, 2414
AvaI CYCGRG 1 cut(s) 3078
AvaII GGWCC 5 cut(s) 28, 229, 1283, 2134, 3355
BaeGI GKGCMC 1 cut(s) 2493
BamHI GGATCC 1 cut(s) 595
BanI GGYRCC 1 cut(s) 2952
BanII GRGCYC 2 cut(s) 2493, 3459
BauI CACGAG 2 cut(s) 1977, 2920
BbsI GAAGAC 2 cut(s) 1125, 1365
Bbv12I GWGCWC 4 cut(s) 1267, 1372, 1597, 3459
BbvCI CCTCAGC 1 cut(s) 2277
BceAI ACGGC 3 cut(s) 65, 1364, 3382
BcgI CGANNNNNNTGC 2 cut(s) 1330, 1364
BciT130I CCWGG 2 cut(s) 2915, 3411
BclI TGATCA 3 cut(s) 274, 3400, 3585
BcnI CCSGG 2 cut(s) 210, 2403
BcoDI GTCTC 6 cut(s) 64, 902, 960, 2216, 2449, 2682
BcuI ACTAGT 1 cut(s) 928
BfmI CTRYAG 2 cut(s) 1230, 3496
BfoI RGCGCY 1 cut(s) 2670
BglII AGATCT 1 cut(s) 1421
BlpI GCTNAGC 1 cut(s) 3045
Bme1390I CCNGG 4 cut(s) 210, 2403, 2915, 3411
Bme18I GGWCC 5 cut(s) 28, 229, 1283, 2134, 3355
BmeT110I CYCGRG 1 cut(s) 3078
BmgT120I GGNCC 8 cut(s) 28, 229, 1283, 1849, 2134, 2489, 2490, 3355
BmrFI CCNGG 4 cut(s) 210, 2403, 2915, 3411
BmrI ACTGGG 1 cut(s) 393
BmsI GCATC 6 cut(s) 342, 2551, 3290, 3415, 3562, 3595
BmtI GCTAGC 2 cut(s) 639, 2418
BmuI ACTGGG 1 cut(s) 393
BoxI GACNNNNGTC 2 cut(s) 56, 3422
BpiI GAAGAC 2 cut(s) 1125, 1365
BplI GAGNNNNNCTC 4 cut(s) 1094, 1126, 2446, 2478
BpmI CTGGAG 4 cut(s) 3024, 3049, 3327, 3432
Bpu10I CCTNAGC 1 cut(s) 2277
Bpu1102I GCTNAGC 1 cut(s) 3045
Bpu14I TTCGAA 2 cut(s) 329, 2484
BpuEI CTTGAG 2 cut(s) 77, 1611
BpuMI CCSGG 2 cut(s) 210, 2403
BsaAI YACGTR 1 cut(s) 3186
BsaBI GATNNNNATC 2 cut(s) 20, 3405
BsaHI GRCGYC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 2449
BsaJI CCNNGG 5 cut(s) 66, 873, 961, 1113, 2899
BsaWI WCCGGW 3 cut(s) 1555, 3211, 3379
BsaXI ACNNNNNCTCC 6 cut(s) 118, 148, 409, 439, 3047, 3077
Bsc4I CCNNNNNNNGG 3 cut(s) 605, 1816, 3365
Bse118I RCCGGY 3 cut(s) 41, 1555, 1908
Bse1I ACTGG 7 cut(s) 109, 399, 1295, 1498, 1527, 3058, 3424
Bse3DI GCAATG 5 cut(s) 679, 1286, 1984, 2154, 2271
Bse8I GATNNNNATC 2 cut(s) 20, 3405
BseAI TCCGGA 1 cut(s) 3379
BseBI CCWGG 2 cut(s) 2915, 3411
BseDI CCNNGG 5 cut(s) 66, 873, 961, 1113, 2899
BseGI GGATG 8 cut(s) 1009, 1523, 2061, 2515, 3406, 3439, 3577, 3610
BseJI GATNNNNATC 2 cut(s) 20, 3405
BseLI CCNNNNNNNGG 3 cut(s) 605, 1816, 3365
BseMI GCAATG 5 cut(s) 679, 1286, 1984, 2154, 2271
BseMII CTCAG 7 cut(s) 75, 1409, 1463, 2067, 2268, 2612, 2690
BseNI ACTGG 7 cut(s) 109, 399, 1295, 1498, 1527, 3058, 3424
BseRI GAGGAG 1 cut(s) 920
BseSI GKGCMC 1 cut(s) 2493
BsgI GTGCAG 3 cut(s) 732, 3281, 3285
Bsh1236I CGCG 1 cut(s) 2206
BshFI GGCC 3 cut(s) 1809, 1850, 2491
BshNI GGYRCC 1 cut(s) 2952
BshTI ACCGGT 1 cut(s) 1555
BsiHKAI GWGCWC 4 cut(s) 1267, 1372, 1597, 3459
BsiHKCI CYCGRG 1 cut(s) 3078
BsiSI CCGG 9 cut(s) 42, 209, 1556, 1810, 1909, 2403, 2442, 3212, 3380
BslFI GGGAC 4 cut(s) 215, 623, 1558, 3430
BslI CCNNNNNNNGG 3 cut(s) 605, 1816, 3365
BsmAI GTCTC 6 cut(s) 64, 902, 960, 2216, 2449, 2682
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 4 cut(s) 215, 623, 1558, 3430
BsmI GAATGC 4 cut(s) 904, 947, 2480, 3525
BsnI GGCC 3 cut(s) 1809, 1850, 2491
Bso31I GGTCTC 1 cut(s) 2449
BsoBI CYCGRG 1 cut(s) 3078
Bsp119I TTCGAA 2 cut(s) 329, 2484
Bsp120I GGGCCC 1 cut(s) 2489
Bsp1286I GDGCHC 5 cut(s) 1267, 1372, 1597, 2493, 3459
Bsp13I TCCGGA 1 cut(s) 3379
Bsp1407I TGTACA 2 cut(s) 3127, 3430
Bsp1720I GCTNAGC 1 cut(s) 3045
Bsp19I CCATGG 1 cut(s) 961
BspACI CCGC 5 cut(s) 123, 336, 466, 1146, 3353
BspANI GGCC 3 cut(s) 1809, 1850, 2491
BspCNI CTCAG 7 cut(s) 74, 1410, 1462, 2068, 2269, 2613, 2691
BspEI TCCGGA 1 cut(s) 3379
BspFNI CGCG 1 cut(s) 2206
BspHI TCATGA 3 cut(s) 714, 979, 3361
BspMAI CTGCAG 2 cut(s) 1234, 3500
BspOI GCTAGC 2 cut(s) 639, 2418
BspPI GGATC 7 cut(s) 278, 590, 603, 1339, 1931, 2702, 3080
BspT104I TTCGAA 2 cut(s) 329, 2484
BspT107I GGYRCC 1 cut(s) 2952
BspTNI GGTCTC 1 cut(s) 2449
BsrDI GCAATG 5 cut(s) 679, 1286, 1984, 2154, 2271
BsrFI RCCGGY 3 cut(s) 41, 1555, 1908
BsrGI TGTACA 2 cut(s) 3127, 3430
BsrI ACTGG 7 cut(s) 109, 399, 1295, 1498, 1527, 3058, 3424
BssAI RCCGGY 3 cut(s) 41, 1555, 1908
BssECI CCNNGG 5 cut(s) 66, 873, 961, 1113, 2899
BssNAI GTATAC 1 cut(s) 3189
BssNI GRCGYC 1 cut(s) 78
BssSI CACGAG 2 cut(s) 1977, 2920
BssT1I CCWWGG 4 cut(s) 873, 961, 1113, 2899
Bst1107I GTATAC 1 cut(s) 3189
Bst2BI CACGAG 2 cut(s) 1977, 2920
Bst2UI CCWGG 2 cut(s) 2915, 3411
Bst4CI ACNGT 4 cut(s) 58, 2084, 2151, 3263
Bst6I CTCTTC 5 cut(s) 155, 464, 2391, 2835, 3631
BstACI GRCGYC 1 cut(s) 78
BstAPI GCANNNNNTGC 3 cut(s) 2342, 3287, 3304
BstAUI TGTACA 2 cut(s) 3127, 3430
BstBAI YACGTR 1 cut(s) 3186
BstBI TTCGAA 2 cut(s) 329, 2484
BstC8I GCNNGC 9 cut(s) 637, 798, 813, 1263, 1910, 2177, 2416, 2434, 3276
BstDSI CCRYGG 1 cut(s) 961
BstF5I GGATG 8 cut(s) 1009, 1523, 2061, 2515, 3406, 3439, 3577, 3610
BstFNI CGCG 1 cut(s) 2206
BstH2I RGCGCY 1 cut(s) 2670
BstHHI GCGC 3 cut(s) 2099, 2347, 2669
BstMAI GTCTC 6 cut(s) 64, 902, 960, 2216, 2449, 2682
BstNI CCWGG 2 cut(s) 2915, 3411
BstNSI RCATGY 6 cut(s) 619, 1355, 1778, 1925, 3278, 3290
BstPAI GACNNNNGTC 2 cut(s) 56, 3422
BstSCI CCNGG 4 cut(s) 208, 2401, 2913, 3409
BstSFI CTRYAG 2 cut(s) 1230, 3496
BstSLI GKGCMC 1 cut(s) 2493
BstSNI TACGTA 1 cut(s) 3186
BstUI CGCG 1 cut(s) 2206
BstV2I GAAGAC 2 cut(s) 1125, 1365
BstX2I RGATCY 4 cut(s) 595, 1421, 3085, 3632
BstYI RGATCY 4 cut(s) 595, 1421, 3085, 3632
BstZ17I GTATAC 1 cut(s) 3189
BsuRI GGCC 3 cut(s) 1809, 1850, 2491
BtgI CCRYGG 1 cut(s) 961
BtsCI GGATG 8 cut(s) 1009, 1523, 2061, 2515, 3406, 3439, 3577, 3610
BtsI GCAGTG 3 cut(s) 1881, 3243, 3493
BtsIMutI CAGTG 8 cut(s) 406, 800, 1534, 1675, 1881, 2618, 3243, 3493
Cac8I GCNNGC 9 cut(s) 637, 798, 813, 1263, 1910, 2177, 2416, 2434, 3276
CciI TCATGA 3 cut(s) 714, 979, 3361
CfoI GCGC 3 cut(s) 2099, 2347, 2669
Cfr10I RCCGGY 3 cut(s) 41, 1555, 1908
Cfr13I GGNCC 8 cut(s) 28, 229, 1283, 1849, 2134, 2489, 2490, 3355
CseI GACGC 2 cut(s) 86, 1721
CsiI ACCWGGT 1 cut(s) 2913
Csp6I GTAC 4 cut(s) 33, 1427, 3128, 3431
CspAI ACCGGT 1 cut(s) 1555
CspCI CAANNNNNGTGG 2 cut(s) 2607, 2642
CviQI GTAC 4 cut(s) 33, 1427, 3128, 3431
DraIII CACNNNGTG 1 cut(s) 3304
EaeI YGGCCR 1 cut(s) 1807
Eam1104I CTCTTC 5 cut(s) 155, 464, 2391, 2835, 3631
EarI CTCTTC 5 cut(s) 155, 464, 2391, 2835, 3631
Ecl136II GAGCTC 1 cut(s) 3457
Eco105I TACGTA 1 cut(s) 3186
Eco130I CCWWGG 4 cut(s) 873, 961, 1113, 2899
Eco24I GRGCYC 2 cut(s) 2493, 3459
Eco31I GGTCTC 1 cut(s) 2449
Eco47I GGWCC 5 cut(s) 28, 229, 1283, 2134, 3355
Eco53kI GAGCTC 1 cut(s) 3457
Eco57I CTGAAG 3 cut(s) 746, 1950, 2201
Eco88I CYCGRG 1 cut(s) 3078
EcoICRI GAGCTC 1 cut(s) 3457
EcoO109I RGGNCCY 2 cut(s) 2489, 2490
EcoRI GAATTC 2 cut(s) 547, 2071
EcoRII CCWGG 2 cut(s) 2913, 3409
EcoT14I CCWWGG 4 cut(s) 873, 961, 1113, 2899
EcoT38I GRGCYC 2 cut(s) 2493, 3459
ErhI CCWWGG 4 cut(s) 873, 961, 1113, 2899
Esp3I CGTCTC 1 cut(s) 64
FaqI GGGAC 4 cut(s) 215, 623, 1558, 3430
FauI CCCGC 1 cut(s) 130
FbaI TGATCA 3 cut(s) 274, 3400, 3585
FblI GTMKAC 1 cut(s) 3188
FokI GGATG 8 cut(s) 1016, 1510, 2048, 2502, 3393, 3426, 3584, 3617
FriOI GRGCYC 2 cut(s) 2493, 3459
GlaI GCGC 3 cut(s) 2098, 2346, 2668
GsuI CTGGAG 4 cut(s) 3024, 3049, 3327, 3432
HaeII RGCGCY 1 cut(s) 2670
HaeIII GGCC 3 cut(s) 1809, 1850, 2491
HapII CCGG 9 cut(s) 42, 209, 1556, 1810, 1909, 2403, 2442, 3212, 3380
HgaI GACGC 2 cut(s) 86, 1721
HhaI GCGC 3 cut(s) 2099, 2347, 2669
Hin1I GRCGYC 1 cut(s) 78
Hin6I GCGC 3 cut(s) 2097, 2345, 2667
HinP1I GCGC 3 cut(s) 2097, 2345, 2667
HincII GTYRAC 2 cut(s) 73, 3163
HindII GTYRAC 2 cut(s) 73, 3163
HindIII AAGCTT 3 cut(s) 2054, 2177, 3047
HpaI GTTAAC 1 cut(s) 3163
HpaII CCGG 9 cut(s) 42, 209, 1556, 1810, 1909, 2403, 2442, 3212, 3380
HphI GGTGA 4 cut(s) 173, 224, 592, 1712
Hpy99I CGWCG 2 cut(s) 80, 820
HpyCH4III ACNGT 4 cut(s) 58, 2084, 2151, 3263
HpyCH4IV ACGT 4 cut(s) 818, 3185, 3191, 3300
HpySE526I ACGT 4 cut(s) 818, 3185, 3191, 3300
Hsp92I GRCGYC 1 cut(s) 78
HspAI GCGC 3 cut(s) 2097, 2345, 2667
Kpn2I TCCGGA 1 cut(s) 3379
KroI GCCGGC 1 cut(s) 1908
KroNI GCCGGC 1 cut(s) 1910
Ksp22I TGATCA 3 cut(s) 274, 3400, 3585
KspAI GTTAAC 1 cut(s) 3163
LmnI GCTCC 6 cut(s) 317, 793, 1865, 3043, 3346, 3454
LweI GCATC 6 cut(s) 342, 2551, 3290, 3415, 3562, 3595
MabI ACCWGGT 1 cut(s) 2913
MaeII ACGT 4 cut(s) 818, 3185, 3191, 3300
MaeIII GTNAC 8 cut(s) 1700, 1877, 2267, 2380, 2917, 3097, 3192, 3323
MfeI CAATTG 3 cut(s) 2100, 2541, 2790
MflI RGATCY 4 cut(s) 595, 1421, 3085, 3632
MhlI GDGCHC 5 cut(s) 1267, 1372, 1597, 2493, 3459
MluI ACGCGT 1 cut(s) 2204
MlyI GAGTC 6 cut(s) 19, 190, 584, 1481, 2252, 3617
MmeI TCCRAC 4 cut(s) 409, 1634, 2347, 3078
MroI TCCGGA 1 cut(s) 3379
MroNI GCCGGC 1 cut(s) 1908
MroXI GAANNNNTTC 3 cut(s) 1122, 2214, 2645
MslI CAYNNNNRTG 7 cut(s) 688, 1133, 1926, 2205, 2376, 2559, 3570
MspI CCGG 9 cut(s) 42, 209, 1556, 1810, 1909, 2403, 2442, 3212, 3380
MspR9I CCNGG 4 cut(s) 210, 2403, 2915, 3411
MunI CAATTG 3 cut(s) 2100, 2541, 2790
Mva1269I GAATGC 4 cut(s) 904, 947, 2480, 3525
MvaI CCWGG 2 cut(s) 2915, 3411
MvnI CGCG 1 cut(s) 2206
NaeI GCCGGC 1 cut(s) 1910
NciI CCSGG 2 cut(s) 210, 2403
NcoI CCATGG 1 cut(s) 961
NgoMIV GCCGGC 1 cut(s) 1908
NheI GCTAGC 2 cut(s) 635, 2414
NmeAIII GCCGAG 1 cut(s) 926
NmuCI GTSAC 4 cut(s) 1700, 1877, 2917, 3192
NspI RCATGY 6 cut(s) 619, 1355, 1778, 1925, 3278, 3290
NspV TTCGAA 2 cut(s) 329, 2484
PaeI GCATGC 1 cut(s) 3278
PagI TCATGA 3 cut(s) 714, 979, 3361
PciI ACATGT 1 cut(s) 1921
PctI GAATGC 4 cut(s) 904, 947, 2480, 3525
PdiI GCCGGC 1 cut(s) 1910
PdmI GAANNNNTTC 3 cut(s) 1122, 2214, 2645
PflFI GACNNNGTC 1 cut(s) 1731
PflMI CCANNNNNTGG 1 cut(s) 605
PfoI TCCNGGA 2 cut(s) 2401, 3409
PinAI ACCGGT 1 cut(s) 1555
PleI GAGTC 6 cut(s) 18, 189, 583, 1480, 2251, 3617
PpsI GAGTC 6 cut(s) 18, 189, 583, 1480, 2251, 3617
Ppu21I YACGTR 1 cut(s) 3186
PscI ACATGT 1 cut(s) 1921
PshAI GACNNNNGTC 2 cut(s) 56, 3422
PsiI TTATAA 1 cut(s) 1860
Psp124BI GAGCTC 1 cut(s) 3459
Psp6I CCWGG 2 cut(s) 2913, 3409
PspGI CCWGG 2 cut(s) 2913, 3409
PspOMI GGGCCC 1 cut(s) 2489
PspPI GGNCC 8 cut(s) 28, 229, 1283, 1849, 2134, 2489, 2490, 3355
PstI CTGCAG 2 cut(s) 1234, 3500
PsuI RGATCY 4 cut(s) 595, 1421, 3085, 3632
PsyI GACNNNGTC 1 cut(s) 1731
RsaI GTAC 4 cut(s) 34, 1428, 3129, 3432
RsaNI GTAC 4 cut(s) 33, 1427, 3128, 3431
RseI CAYNNNNRTG 7 cut(s) 688, 1133, 1926, 2205, 2376, 2559, 3570
SacI GAGCTC 1 cut(s) 3459
Sau96I GGNCC 8 cut(s) 28, 229, 1283, 1849, 2134, 2489, 2490, 3355
SchI GAGTC 6 cut(s) 19, 190, 584, 1481, 2252, 3617
ScrFI CCNGG 4 cut(s) 210, 2403, 2915, 3411
SduI GDGCHC 5 cut(s) 1267, 1372, 1597, 2493, 3459
SexAI ACCWGGT 1 cut(s) 2913
SfaNI GCATC 6 cut(s) 342, 2551, 3290, 3415, 3562, 3595
SfcI CTRYAG 2 cut(s) 1230, 3496
SfuI TTCGAA 2 cut(s) 329, 2484
SinI GGWCC 5 cut(s) 28, 229, 1283, 2134, 3355
SmiMI CAYNNNNRTG 7 cut(s) 688, 1133, 1926, 2205, 2376, 2559, 3570
SmlI CTYRAG 2 cut(s) 92, 1626
SmoI CTYRAG 2 cut(s) 92, 1626
SnaBI TACGTA 1 cut(s) 3186
SpeI ACTAGT 1 cut(s) 928
SphI GCATGC 1 cut(s) 3278
SsiI CCGC 5 cut(s) 123, 336, 466, 1146, 3353
SspI AATATT 1 cut(s) 2504
SstI GAGCTC 1 cut(s) 3459
StyD4I CCNGG 4 cut(s) 208, 2401, 2913, 3409
StyI CCWWGG 4 cut(s) 873, 961, 1113, 2899
TaaI ACNGT 4 cut(s) 58, 2084, 2151, 3263
TaiI ACGT 4 cut(s) 821, 3188, 3194, 3303
TaqII GACCGA 1 cut(s) 58
TatI WGTACW 3 cut(s) 1426, 3127, 3430
TscAI CASTG 8 cut(s) 406, 807, 1534, 1675, 1888, 2625, 3243, 3500
TseFI GTSAC 4 cut(s) 1700, 1877, 2917, 3192
Tsp45I GTSAC 4 cut(s) 1700, 1877, 2917, 3192
TspGWI ACGGA 1 cut(s) 20
TspRI CASTG 8 cut(s) 406, 807, 1534, 1675, 1888, 2625, 3243, 3500
Tth111I GACNNNGTC 1 cut(s) 1731
Van91I CCANNNNNTGG 1 cut(s) 605
VpaK11BI GGWCC 5 cut(s) 28, 229, 1283, 2134, 3355
XapI RAATTY 5 cut(s) 547, 1802, 2002, 2071, 2211
XbaI TCTAGA 2 cut(s) 551, 2014
XceI RCATGY 6 cut(s) 619, 1355, 1778, 1925, 3278, 3290
XcmI CCANNNNNNNNNTGG 1 cut(s) 1802
XmiI GTMKAC 1 cut(s) 3188
XmnI GAANNNNTTC 3 cut(s) 1122, 2214, 2645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.