Rmu_sc0003887.1_g000008

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003887.1
OG0013774
Physical Location & Seq
Reverse (-)
31262 .. 31672
411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003887.1_g000008.1.cds

Sequence Viewer

Length: 411 bp
atgagagctctcgctctctgttccttgcggtggctaatcctagggttttgctgttcgttttctttggcctgcgtcaatggtgttcgcggtcactgcccgacgagttctctggactcatgtacatttccctttcaaccatccggttgctgcaccctcatctcattggtttattggtgggatcctccctctttgggtttttctcgtgcctctgcctcccttttcagaggtggtgacggacggcgaagccgtggtttggtggatccaaaccagacgtgggatccgggatctaccaagtggaggcgtctggtaagaggtacgatcggactttcttcagcgataacgattcggctcctggttgttgttgaaatcctgcgatggttggagaagctgcttttgatttctctacgttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

15.09

Weight (kDa)

9.76

Isoelectric Point (pI)

59.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 87
AciI CCGC 2 cut(s) 28, 87
AclWI GGATC 7 cut(s) 173, 186, 254, 267, 272, 285, 292
AcuI CTGAAG 1 cut(s) 315
AcyI GRCGYC 1 cut(s) 301
AfaI GTAC 2 cut(s) 121, 316
AfiI CCNNNNNNNGG 5 cut(s) 30, 191, 253, 274, 297
AgsI TTSAA 2 cut(s) 134, 365
AjiI CACGTC 1 cut(s) 273
AjnI CCWGG 1 cut(s) 351
AluBI AGCT 2 cut(s) 8, 388
AluI AGCT 2 cut(s) 8, 388
Alw21I GWGCWC 1 cut(s) 10
AlwI GGATC 7 cut(s) 173, 186, 254, 267, 272, 285, 292
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 2 cut(s) 147, 388
AspA2I CCTAGG 1 cut(s) 40
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 1 cut(s) 242
AvrII CCTAGG 1 cut(s) 40
BamHI GGATCC 3 cut(s) 178, 259, 277
BanII GRGCYC 1 cut(s) 10
BauI CACGAG 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 10
BbvI GCAGC 2 cut(s) 134, 375
BccI CCATC 2 cut(s) 145, 369
BceAI ACGGC 2 cut(s) 231, 254
BciT130I CCWGG 1 cut(s) 353
BcnI CCSGG 1 cut(s) 282
BfaI CTAG 1 cut(s) 41
BisI GCNGC 2 cut(s) 148, 389
BlnI CCTAGG 1 cut(s) 40
BlsI GCNGC 2 cut(s) 149, 390
Bme1390I CCNGG 2 cut(s) 282, 353
BmgBI CACGTC 1 cut(s) 273
BmiI GGNNCC 4 cut(s) 180, 261, 279, 350
BmrFI CCNGG 2 cut(s) 282, 353
BpuMI CCSGG 1 cut(s) 282
BsaHI GRCGYC 1 cut(s) 301
BsaJI CCNNGG 2 cut(s) 40, 247
BsaWI WCCGGW 1 cut(s) 140
Bsc4I CCNNNNNNNGG 5 cut(s) 30, 191, 253, 274, 297
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 2 cut(s) 40, 247
BseGI GGATG 1 cut(s) 137
BseLI CCNNNNNNNGG 5 cut(s) 30, 191, 253, 274, 297
BseXI GCAGC 2 cut(s) 134, 375
BsgI GTGCAG 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 321
BshFI GGCC 1 cut(s) 68
BsiEI CGRYCG 1 cut(s) 321
BsiHKAI GWGCWC 1 cut(s) 10
BsiSI CCGG 2 cut(s) 141, 281
BslI CCNNNNNNNGG 5 cut(s) 30, 191, 253, 274, 297
BsnI GGCC 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 10
Bsp1407I TGTACA 1 cut(s) 119
Bsp143I GATC 5 cut(s) 178, 259, 277, 284, 318
BspACI CCGC 2 cut(s) 28, 87
BspANI GGCC 1 cut(s) 68
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 4 cut(s) 180, 261, 279, 350
BspPI GGATC 7 cut(s) 173, 186, 254, 267, 272, 285, 292
BsrGI TGTACA 1 cut(s) 119
BssECI CCNNGG 2 cut(s) 40, 247
BssMI GATC 5 cut(s) 178, 259, 277, 284, 318
BssNI GRCGYC 1 cut(s) 301
BssSI CACGAG 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 40
Bst2BI CACGAG 1 cut(s) 201
Bst2UI CCWGG 1 cut(s) 353
BstACI GRCGYC 1 cut(s) 301
BstAUI TGTACA 1 cut(s) 119
BstC8I GCNNGC 1 cut(s) 70
BstDSI CCRYGG 1 cut(s) 247
BstF5I GGATG 1 cut(s) 137
BstFNI CGCG 1 cut(s) 87
BstKTI GATC 5 cut(s) 181, 262, 280, 287, 321
BstMBI GATC 5 cut(s) 178, 259, 277, 284, 318
BstMCI CGRYCG 1 cut(s) 321
BstMWI GCNNNNNNNGC 1 cut(s) 93
BstNI CCWGG 1 cut(s) 353
BstSCI CCNGG 2 cut(s) 280, 351
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 2 cut(s) 134, 375
BstX2I RGATCY 4 cut(s) 178, 259, 277, 284
BstYI RGATCY 4 cut(s) 178, 259, 277, 284
BsuRI GGCC 1 cut(s) 68
BtgI CCRYGG 1 cut(s) 247
BtgZI GCGATG 1 cut(s) 388
BtrI CACGTC 1 cut(s) 273
BtsCI GGATG 1 cut(s) 137
BtsI GCAGTG 1 cut(s) 91
BtsIMutI CAGTG 1 cut(s) 91
Cac8I GCNNGC 1 cut(s) 70
CseI GACGC 2 cut(s) 61, 290
Csp6I GTAC 2 cut(s) 120, 315
CviAII CATG 1 cut(s) 117
CviJI RGCY 6 cut(s) 8, 34, 68, 246, 349, 388
CviKI_1 RGCY 6 cut(s) 8, 34, 68, 246, 349, 388
CviQI GTAC 2 cut(s) 120, 315
DpnI GATC 5 cut(s) 180, 261, 279, 286, 320
DpnII GATC 5 cut(s) 178, 259, 277, 284, 318
Ecl136II GAGCTC 1 cut(s) 8
Eco130I CCWWGG 1 cut(s) 40
Eco24I GRGCYC 1 cut(s) 10
Eco53kI GAGCTC 1 cut(s) 8
Eco57I CTGAAG 1 cut(s) 315
EcoICRI GAGCTC 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 351
EcoT14I CCWWGG 1 cut(s) 40
EcoT38I GRGCYC 1 cut(s) 10
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 1 cut(s) 120
FaiI YATR 1 cut(s) 118
FatI CATG 1 cut(s) 116
Fnu4HI GCNGC 2 cut(s) 148, 389
FokI GGATG 1 cut(s) 124
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 2 cut(s) 148, 389
FspBI CTAG 1 cut(s) 41
GluI GCNGC 2 cut(s) 148, 389
HaeIII GGCC 1 cut(s) 68
HapII CCGG 2 cut(s) 141, 281
HgaI GACGC 2 cut(s) 61, 290
Hin1I GRCGYC 1 cut(s) 301
Hin1II CATG 1 cut(s) 120
HinfI GANTC 2 cut(s) 113, 343
HpaII CCGG 2 cut(s) 141, 281
HphI GGTGA 1 cut(s) 242
Hpy188I TCNGA 2 cut(s) 224, 323
Hpy188III TCNNGA 1 cut(s) 110
Hpy99I CGWCG 1 cut(s) 103
HpyCH4IV ACGT 2 cut(s) 272, 406
HpyCH4V TGCA 1 cut(s) 150
HpyF10VI GCNNNNNNNGC 1 cut(s) 93
HpySE526I ACGT 2 cut(s) 272, 406
Hsp92I GRCGYC 1 cut(s) 301
Hsp92II CATG 1 cut(s) 120
Kzo9I GATC 5 cut(s) 178, 259, 277, 284, 318
LmnI GCTCC 1 cut(s) 354
LpnPI CCDG 9 cut(s) 82, 95, 154, 281, 290, 294, 338, 365, 383
Lsp1109I GCAGC 2 cut(s) 134, 375
MaeI CTAG 1 cut(s) 41
MaeII ACGT 2 cut(s) 272, 406
MaeIII GTNAC 2 cut(s) 89, 230
MalI GATC 5 cut(s) 180, 261, 279, 286, 320
MboI GATC 5 cut(s) 178, 259, 277, 284, 318
MboII GAAGA 1 cut(s) 321
MflI RGATCY 4 cut(s) 178, 259, 277, 284
MhlI GDGCHC 1 cut(s) 10
MlyI GAGTC 1 cut(s) 107
MmeI TCCRAC 1 cut(s) 360
MnlI CCTC 8 cut(s) 164, 192, 196, 217, 218, 223, 291, 305
MspI CCGG 2 cut(s) 141, 281
MspR9I CCNGG 2 cut(s) 282, 353
MvaI CCWGG 1 cut(s) 353
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 1 cut(s) 93
NciI CCSGG 1 cut(s) 282
NdeII GATC 5 cut(s) 178, 259, 277, 284, 318
NlaIII CATG 1 cut(s) 120
NlaIV GGNNCC 4 cut(s) 180, 261, 279, 350
NmuCI GTSAC 2 cut(s) 89, 230
PcsI WCGNNNNNNNCGW 1 cut(s) 244
PfeI GAWTC 1 cut(s) 343
PfoI TCCNGGA 1 cut(s) 280
PkrI GCNGC 2 cut(s) 149, 390
Ple19I CGATCG 1 cut(s) 321
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
Psp124BI GAGCTC 1 cut(s) 10
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 4 cut(s) 180, 261, 279, 350
PsuI RGATCY 4 cut(s) 178, 259, 277, 284
PvuI CGATCG 1 cut(s) 321
RsaI GTAC 2 cut(s) 121, 316
RsaNI GTAC 2 cut(s) 120, 315
SacI GAGCTC 1 cut(s) 10
SatI GCNGC 2 cut(s) 148, 389
Sau3AI GATC 5 cut(s) 178, 259, 277, 284, 318
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 2 cut(s) 282, 353
SduI GDGCHC 1 cut(s) 10
SetI ASST 6 cut(s) 10, 229, 275, 316, 390, 409
SsiI CCGC 2 cut(s) 28, 87
SspMI CTAG 1 cut(s) 41
SstI GAGCTC 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 280, 351
StyI CCWWGG 1 cut(s) 40
TaiI ACGT 2 cut(s) 275, 409
TatI WGTACW 1 cut(s) 119
TfiI GAWTC 1 cut(s) 343
TscAI CASTG 1 cut(s) 98
TseFI GTSAC 2 cut(s) 89, 230
TseI GCWGC 2 cut(s) 147, 388
Tsp45I GTSAC 2 cut(s) 89, 230
TspGWI ACGGA 1 cut(s) 249
TspRI CASTG 1 cut(s) 98
XcmI CCANNNNNNNNNTGG 1 cut(s) 270
XmaJI CCTAGG 1 cut(s) 40
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.