Rh6DG492500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0013774
Physical Location & Seq
Reverse (-)
65202711 .. 65203592
882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG492500.1

Sequence Viewer

Length: 459 bp
ATGGTGGCGGCAGTGCGTTGGGTTGTATGGCCTCATGGTGTTCCTCAATCGGTTCCAAGATCGGCGATCTCCGTGGGCTGGTGGTGGCTCCGAGTTCTAGGAAGCGACAATCACTCGGGGAGGACGAAAGCCGCGGCGGGTGATGGAGATGGTGTACTCCAACGGTGGCCTGACTTAGTGTGGCGGCGTTGGGATGGTGGTCTGGGTGCCCTGAGTATTCCTTCAGGGGCTTGGGCTTGGTCCGACTTTCTGGTGTCATGGGCTGCTCTAGGGCCTGGTGGTTCGGGGTGGGCTGTGTTGGTTAGGCCCGCTCACTTTTGGGCCCGTATTTGGGATCCAGGTGGATTGGGGACTAAGGGAAGGGAGAGGTATGCCGAGAGCAGTAAGAAGGGTCCGGTCCAACAGGAGGTAACGGATCACTTGGGGAATGGAATGAAGAGCCCATTTGGAATTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

16.55

Weight (kDa)

9.97

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 206
AccBSI CCGCTC 1 cut(s) 311
AccII CGCG 1 cut(s) 134
AciI CCGC 6 cut(s) 8, 132, 134, 137, 184, 309
AclWI GGATC 3 cut(s) 329, 342, 423
AcuI CTGAAG 1 cut(s) 207
AfaI GTAC 1 cut(s) 156
AfiI CCNNNNNNNGG 4 cut(s) 78, 330, 331, 406
AgsI TTSAA 1 cut(s) 455
AjnI CCWGG 2 cut(s) 274, 337
AlwI GGATC 3 cut(s) 329, 342, 423
Ama87I CYCGRG 1 cut(s) 115
AoxI GGCC 5 cut(s) 29, 167, 272, 305, 321
ApaI GGGCCC 1 cut(s) 325
ApeKI GCWGC 1 cut(s) 263
AspS9I GGNCC 7 cut(s) 240, 272, 306, 321, 322, 392, 397
AsuHPI GGTGA 1 cut(s) 152
AvaI CYCGRG 1 cut(s) 115
AvaII GGWCC 3 cut(s) 240, 392, 397
BaeGI GKGCMC 2 cut(s) 211, 325
BamHI GGATCC 1 cut(s) 334
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 2 cut(s) 325, 443
BbvI GCAGC 1 cut(s) 250
BccI CCATC 3 cut(s) 137, 143, 188
BciT130I CCWGG 2 cut(s) 276, 339
BfaI CTAG 2 cut(s) 98, 269
BisI GCNGC 5 cut(s) 9, 132, 135, 185, 264
BlsI GCNGC 5 cut(s) 10, 133, 136, 186, 265
Bme1390I CCNGG 2 cut(s) 276, 339
Bme18I GGWCC 3 cut(s) 240, 392, 397
BmeT110I CYCGRG 1 cut(s) 115
BmgT120I GGNCC 7 cut(s) 240, 272, 306, 321, 322, 392, 397
BmiI GGNNCC 6 cut(s) 54, 89, 208, 323, 336, 393
BmrFI CCNGG 2 cut(s) 276, 339
BsaJI CCNNGG 2 cut(s) 72, 132
BsaWI WCCGGW 1 cut(s) 394
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bsc4I CCNNNNNNNGG 4 cut(s) 78, 330, 331, 406
BseBI CCWGG 2 cut(s) 276, 339
BseDI CCNNGG 2 cut(s) 72, 132
BseGI GGATG 1 cut(s) 199
BseLI CCNNNNNNNGG 4 cut(s) 78, 330, 331, 406
BseMII CTCAG 1 cut(s) 203
BseSI GKGCMC 2 cut(s) 211, 325
BseXI GCAGC 1 cut(s) 250
Bsh1236I CGCG 1 cut(s) 134
BshFI GGCC 5 cut(s) 31, 169, 274, 307, 323
BshNI GGYRCC 1 cut(s) 206
BsiHKCI CYCGRG 1 cut(s) 115
BsiSI CCGG 1 cut(s) 395
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 4 cut(s) 78, 330, 331, 406
BsmFI GGGAC 1 cut(s) 364
BsnI GGCC 5 cut(s) 31, 169, 274, 307, 323
BsoBI CYCGRG 1 cut(s) 115
Bsp120I GGGCCC 1 cut(s) 321
Bsp1286I GDGCHC 3 cut(s) 211, 325, 443
Bsp143I GATC 4 cut(s) 59, 66, 334, 415
BspACI CCGC 6 cut(s) 8, 132, 134, 137, 184, 309
BspANI GGCC 5 cut(s) 31, 169, 274, 307, 323
BspCNI CTCAG 1 cut(s) 204
BspFNI CGCG 1 cut(s) 134
BspLI GGNNCC 6 cut(s) 54, 89, 208, 323, 336, 393
BspPI GGATC 3 cut(s) 329, 342, 423
BspQI GCTCTTC 1 cut(s) 431
BspT107I GGYRCC 1 cut(s) 206
BsrBI CCGCTC 1 cut(s) 311
BssECI CCNNGG 2 cut(s) 72, 132
BssMI GATC 4 cut(s) 59, 66, 334, 415
Bst2UI CCWGG 2 cut(s) 276, 339
Bst4CI ACNGT 1 cut(s) 165
Bst6I CTCTTC 1 cut(s) 431
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 3 cut(s) 175, 212, 354
BstDSI CCRYGG 2 cut(s) 72, 132
BstF5I GGATG 1 cut(s) 199
BstFNI CGCG 1 cut(s) 134
BstKTI GATC 4 cut(s) 62, 69, 337, 418
BstMBI GATC 4 cut(s) 59, 66, 334, 415
BstNI CCWGG 2 cut(s) 276, 339
BstSCI CCNGG 2 cut(s) 274, 337
BstSLI GKGCMC 2 cut(s) 211, 325
BstUI CGCG 1 cut(s) 134
BstV1I GCAGC 1 cut(s) 250
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BsuRI GGCC 5 cut(s) 31, 169, 274, 307, 323
BtgI CCRYGG 2 cut(s) 72, 132
BtsCI GGATG 1 cut(s) 199
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 1 cut(s) 309
Cfr13I GGNCC 7 cut(s) 240, 272, 306, 321, 322, 392, 397
Cfr42I CCGCGG 1 cut(s) 135
Csp6I GTAC 1 cut(s) 155
CviAII CATG 2 cut(s) 35, 258
CviQI GTAC 1 cut(s) 155
DdeI CTNAG 3 cut(s) 175, 212, 354
DpnI GATC 4 cut(s) 61, 68, 336, 417
DpnII GATC 4 cut(s) 59, 66, 334, 415
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
Eco24I GRGCYC 2 cut(s) 325, 443
Eco47I GGWCC 3 cut(s) 240, 392, 397
Eco57I CTGAAG 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 115
EcoO109I RGGNCCY 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 274, 337
EcoT38I GRGCYC 2 cut(s) 325, 443
FaeI CATG 2 cut(s) 38, 261
FaiI YATR 4 cut(s) 28, 36, 259, 372
FaqI GGGAC 1 cut(s) 364
FatI CATG 2 cut(s) 34, 257
FauI CCCGC 2 cut(s) 130, 316
Fnu4HI GCNGC 5 cut(s) 9, 132, 135, 185, 264
FokI GGATG 1 cut(s) 206
FriOI GRGCYC 2 cut(s) 325, 443
Fsp4HI GCNGC 5 cut(s) 9, 132, 135, 185, 264
FspBI CTAG 2 cut(s) 98, 269
GluI GCNGC 5 cut(s) 9, 132, 135, 185, 264
HaeIII GGCC 5 cut(s) 31, 169, 274, 307, 323
HapII CCGG 1 cut(s) 395
Hin1II CATG 2 cut(s) 38, 261
HpaII CCGG 1 cut(s) 395
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 1 cut(s) 155
Hpy188I TCNGA 2 cut(s) 92, 244
Hpy8I GTNNAC 1 cut(s) 155
HpyAV CCTTC 3 cut(s) 231, 354, 382
HpyCH4III ACNGT 1 cut(s) 165
HpyF3I CTNAG 3 cut(s) 175, 212, 354
Hsp92II CATG 2 cut(s) 38, 261
KspI CCGCGG 1 cut(s) 135
Kzo9I GATC 4 cut(s) 59, 66, 334, 415
LguI GCTCTTC 1 cut(s) 431
LmnI GCTCC 1 cut(s) 93
Lsp1109I GCAGC 1 cut(s) 250
MaeI CTAG 2 cut(s) 98, 269
MaeIII GTNAC 1 cut(s) 409
MalI GATC 4 cut(s) 61, 68, 336, 417
MbiI CCGCTC 1 cut(s) 311
MboI GATC 4 cut(s) 59, 66, 334, 415
MboII GAAGA 1 cut(s) 448
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 3 cut(s) 211, 325, 443
MluCI AATT 1 cut(s) 450
MmeI TCCRAC 3 cut(s) 184, 267, 424
MnlI CCTC 5 cut(s) 42, 54, 114, 360, 400
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 2 cut(s) 276, 339
MvaI CCWGG 2 cut(s) 276, 339
MvnI CGCG 1 cut(s) 134
NdeII GATC 4 cut(s) 59, 66, 334, 415
NlaIII CATG 2 cut(s) 38, 261
NlaIV GGNNCC 6 cut(s) 54, 89, 208, 323, 336, 393
NmeAIII GCCGAG 1 cut(s) 400
PciSI GCTCTTC 1 cut(s) 431
PcsI WCGNNNNNNNCGW 1 cut(s) 122
PkrI GCNGC 5 cut(s) 10, 133, 136, 186, 265
Psp6I CCWGG 2 cut(s) 274, 337
PspGI CCWGG 2 cut(s) 274, 337
PspN4I GGNNCC 6 cut(s) 54, 89, 208, 323, 336, 393
PspOMI GGGCCC 1 cut(s) 321
PspPI GGNCC 7 cut(s) 240, 272, 306, 321, 322, 392, 397
PsuI RGATCY 1 cut(s) 334
RsaI GTAC 1 cut(s) 156
RsaNI GTAC 1 cut(s) 155
SacII CCGCGG 1 cut(s) 135
SapI GCTCTTC 1 cut(s) 431
SatI GCNGC 5 cut(s) 9, 132, 135, 185, 264
Sau3AI GATC 4 cut(s) 59, 66, 334, 415
Sau96I GGNCC 7 cut(s) 240, 272, 306, 321, 322, 392, 397
ScrFI CCNGG 2 cut(s) 276, 339
SduI GDGCHC 3 cut(s) 211, 325, 443
SetI ASST 3 cut(s) 343, 371, 411
Sfr303I CCGCGG 1 cut(s) 135
SgrBI CCGCGG 1 cut(s) 135
SinI GGWCC 3 cut(s) 240, 392, 397
Sse9I AATT 1 cut(s) 450
SsiI CCGC 6 cut(s) 8, 132, 134, 137, 184, 309
SspMI CTAG 2 cut(s) 98, 269
StyD4I CCNGG 2 cut(s) 274, 337
TaaI ACNGT 1 cut(s) 165
TasI AATT 1 cut(s) 450
TatI WGTACW 1 cut(s) 154
TauI GCSGC 4 cut(s) 11, 134, 137, 187
TscAI CASTG 1 cut(s) 18
TseI GCWGC 1 cut(s) 263
TspDTI ATGAA 1 cut(s) 449
TspGWI ACGGA 2 cut(s) 61, 428
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 3 cut(s) 240, 392, 397
XspI CTAG 2 cut(s) 98, 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.