Rmu_sc0004189.1_g000039

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004189.1
OG0014820
Physical Location & Seq
Reverse (-)
182534 .. 182884
351 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 351 bp
atgccatacttaaggcgtataaattcaaatacctcctcaaagatctatgctagcaggacactgcttttcttgagaagtgatggaactttccaaccattggtgattgaactgagcttgcctcatcctgatggagatcaactcagtcgcattagcagtgtgctcacaccagctgaaggaggtgtggaaagctccatatggcagctggccaaagcttatgttgccgtaaatgactctggagttcatcaacttatcagtcactggtattactcttttaaacaatccttggggaacaagttcatctttgaaaatttgacagaagtcttatccaactgcagaaaccagaagatatag

Protein Analysis

116

Amino Acids

13.1

Weight (kDa)

8.82

Isoelectric Point (pI)

54.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipoxygenase PF00305 1 - 89 1.2e-32 Lipoxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 97
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 2 cut(s) 22, 307
AcuI CTGAAG 1 cut(s) 192
AfiI CCNNNNNNNGG 2 cut(s) 97, 173
AflII CTTAAG 1 cut(s) 10
AgsI TTSAA 3 cut(s) 27, 107, 305
AluBI AGCT 5 cut(s) 114, 170, 189, 202, 212
AluI AGCT 5 cut(s) 114, 170, 189, 202, 212
Alw21I GWGCWC 1 cut(s) 162
AlwNI CAGNNNCTG 1 cut(s) 258
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 1 cut(s) 199
ApoI RAATTY 2 cut(s) 22, 307
Asp700I GAANNNNTTC 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 112
AsuNHI GCTAGC 1 cut(s) 50
BalI TGGCCA 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 211
BccI CCATC 2 cut(s) 74, 122
BceAI ACGGC 1 cut(s) 206
BfaI CTAG 1 cut(s) 51
BfmI CTRYAG 1 cut(s) 331
BfrI CTTAAG 1 cut(s) 10
BglII AGATCT 1 cut(s) 42
BisI GCNGC 1 cut(s) 200
BlsI GCNGC 1 cut(s) 201
BmtI GCTAGC 1 cut(s) 54
BoxI GACNNNNGTC 1 cut(s) 317
BplI GAGNNNNNCTC 4 cut(s) 103, 135, 123, 155
BpmI CTGGAG 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 91
BsaBI GATNNNNATC 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 282
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 173
Bse1I ACTGG 1 cut(s) 263
Bse8I GATNNNNATC 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 282
BseGI GGATG 1 cut(s) 121
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 97, 173
BseMII CTCAG 2 cut(s) 101, 154
BseNI ACTGG 1 cut(s) 263
BseRI GAGGAG 1 cut(s) 25
BseXI GCAGC 1 cut(s) 211
BshFI GGCC 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 162
BslI CCNNNNNNNGG 2 cut(s) 97, 173
BsnI GGCC 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 2 cut(s) 42, 133
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 2 cut(s) 102, 153
BspMAI CTGCAG 1 cut(s) 335
BspOI GCTAGC 1 cut(s) 54
BspTI CTTAAG 1 cut(s) 10
BsrI ACTGG 1 cut(s) 263
BssECI CCNNGG 1 cut(s) 282
BssMI GATC 2 cut(s) 42, 133
BssT1I CCWWGG 1 cut(s) 282
BstAFI CTTAAG 1 cut(s) 10
BstC8I GCNNGC 3 cut(s) 52, 116, 204
BstDEI CTNAG 2 cut(s) 110, 140
BstF5I GGATG 1 cut(s) 121
BstKTI GATC 2 cut(s) 45, 136
BstMBI GATC 2 cut(s) 42, 133
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstPAI GACNNNNGTC 1 cut(s) 317
BstSFI CTRYAG 1 cut(s) 331
BstV1I GCAGC 1 cut(s) 211
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
BsuRI GGCC 1 cut(s) 206
BtsCI GGATG 1 cut(s) 121
BtsI GCAGTG 2 cut(s) 59, 160
BtsIMutI CAGTG 3 cut(s) 59, 160, 256
Cac8I GCNNGC 3 cut(s) 52, 116, 204
CaiI CAGNNNCTG 1 cut(s) 258
CviJI RGCY 6 cut(s) 114, 170, 189, 202, 206, 212
CviKI_1 RGCY 6 cut(s) 114, 170, 189, 202, 206, 212
DdeI CTNAG 2 cut(s) 110, 140
DpnI GATC 2 cut(s) 44, 135
DpnII GATC 2 cut(s) 42, 133
DraI TTTAAA 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 282
Eco57I CTGAAG 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 282
FaiI YATR 7 cut(s) 7, 20, 48, 194, 196, 216, 349
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FauNDI CATATG 1 cut(s) 194
Fnu4HI GCNGC 1 cut(s) 200
FokI GGATG 1 cut(s) 108
Fsp4HI GCNGC 1 cut(s) 200
FspBI CTAG 1 cut(s) 51
GluI GCNGC 1 cut(s) 200
GsuI CTGGAG 1 cut(s) 255
HaeIII GGCC 1 cut(s) 206
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 1 cut(s) 230
HphI GGTGA 1 cut(s) 112
Hpy188III TCNNGA 3 cut(s) 70, 125, 234
HpyAV CCTTC 1 cut(s) 167
HpyCH4V TGCA 1 cut(s) 333
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 2 cut(s) 110, 140
Kzo9I GATC 2 cut(s) 42, 133
LmnI GCTCC 1 cut(s) 194
LpnPI CCDG 6 cut(s) 40, 138, 180, 188, 219, 244
Lsp1109I GCAGC 1 cut(s) 211
MaeI CTAG 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 254
MalI GATC 2 cut(s) 44, 135
MboI GATC 2 cut(s) 42, 133
MflI RGATCY 1 cut(s) 42
MhlI GDGCHC 1 cut(s) 162
MlsI TGGCCA 1 cut(s) 206
MluCI AATT 2 cut(s) 22, 307
MluNI TGGCCA 1 cut(s) 206
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 2 cut(s) 115, 351
MnlI CCTC 4 cut(s) 43, 46, 129, 170
Mox20I TGGCCA 1 cut(s) 206
MroXI GAANNNNTTC 1 cut(s) 293
MscI TGGCCA 1 cut(s) 206
MseI TTAA 2 cut(s) 11, 273
MslI CAYNNNNRTG 1 cut(s) 126
Msp20I TGGCCA 1 cut(s) 206
MspA1I CMGCKG 2 cut(s) 170, 202
MspCI CTTAAG 1 cut(s) 10
MwoI GCNNNNNNNGC 1 cut(s) 218
NdeI CATATG 1 cut(s) 194
NdeII GATC 2 cut(s) 42, 133
NheI GCTAGC 1 cut(s) 50
NmuCI GTSAC 1 cut(s) 254
PdmI GAANNNNTTC 1 cut(s) 293
PflMI CCANNNNNTGG 1 cut(s) 97
PkrI GCNGC 1 cut(s) 201
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
PshAI GACNNNNGTC 1 cut(s) 317
PstI CTGCAG 1 cut(s) 335
PstNI CAGNNNCTG 1 cut(s) 258
PsuI RGATCY 1 cut(s) 42
PvuII CAGCTG 2 cut(s) 170, 202
RseI CAYNNNNRTG 1 cut(s) 126
SaqAI TTAA 2 cut(s) 11, 273
SatI GCNGC 1 cut(s) 200
Sau3AI GATC 2 cut(s) 42, 133
SchI GAGTC 1 cut(s) 224
SduI GDGCHC 1 cut(s) 162
SetI ASST 7 cut(s) 35, 116, 172, 181, 191, 204, 214
SfcI CTRYAG 1 cut(s) 331
SmiMI CAYNNNNRTG 1 cut(s) 126
SmlI CTYRAG 2 cut(s) 10, 70
SmoI CTYRAG 2 cut(s) 10, 70
Sse9I AATT 2 cut(s) 22, 307
SspMI CTAG 1 cut(s) 51
StyI CCWWGG 1 cut(s) 282
TasI AATT 2 cut(s) 22, 307
Tru1I TTAA 2 cut(s) 11, 273
Tru9I TTAA 2 cut(s) 11, 273
TscAI CASTG 3 cut(s) 66, 160, 263
TseFI GTSAC 1 cut(s) 254
TseI GCWGC 1 cut(s) 199
Tsp45I GTSAC 1 cut(s) 254
TspDTI ATGAA 2 cut(s) 230, 286
TspRI CASTG 3 cut(s) 66, 160, 263
Van91I CCANNNNNTGG 1 cut(s) 97
Vha464I CTTAAG 1 cut(s) 10
XapI RAATTY 2 cut(s) 22, 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.