Rmu_sc0004577.1_g000021

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004577.1
OG0007184
Physical Location & Seq
Reverse (-)
78818 .. 82016
3199 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1200 bp
atgtctgggctcaccttgggcctcatgtccctgagcctcgtcgatcttgaagtcttggccaagtcagggaagcccagcagtcgaaagcatgctgcaaagatattgccagttgtgaaaaagcagcacctgttgctttgcaccctactaatttgcaatgctgctgcgatggaggcacttcccattttcctggatggtttagtgccagcttggggtgcaattctaatttcggtgacactaattcttctgtttggtgagattataccacaatctgtttgttcccgttatggtttggcgataggtgcagcagtgactccatttgtccgtgtccttctgtgggtctgctttcctcttgcatatccaattagcaagttattagactatcttctcggccatggacatgtagctctgtttcgcagagcggagttgaaaacgcttgtagatatgcatggcaatgaggccggaaaaggtggagaattgacacatgatgagacaacaattattgctggagcactcgaactcagtgagaaaactgcccgtgacgccatgactcctatagctgaaacttttgcaattgatatcaatgccaagcttgacaggaatttgatgaatctgataatggagaaagggcatagtagagtgccagtttactatgaagagcctacaaacataattggacttgtcctggtcaaaaacttgttgacagttcatccagaagaggaaacacctgtaaagaatgtcactatacgcaagatcccaagggttcgagaaacattgccgttgtatgacattctgaatgaatttcagaaaggtcacagtcacatggctattgttataagacggtgcaatactgaggagcagcccaatggcaatacagctgacagtccagtgaaagaagtgaaagtggacattgatggtgaaaagcctcctcaggagaagattttgaagagcaaaagatcactgaacaaatggaagagctttccgaacagtgggaatatttcacagaggagtgggtccagaagcaagaaatggtccaaggacatgtattcagacatccttgagatagatagtgaagcactcccaaagctcccagaagaggaagtggctgttggcattataacaatggaagacgttatcgaagagcttttacaggaggagatatttgatgaaactgatcatcagtatgaggactcatactcataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

399

Amino Acids

44.33

Weight (kDa)

5.65

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 1 - 161 2e-30 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 833, 1115
AccBSI CCGCTC 1 cut(s) 419
AciI CCGC 1 cut(s) 419
AclWI GGATC 1 cut(s) 745
AcoI YGGCCR 2 cut(s) 57, 388
AcsI RAATTY 2 cut(s) 598, 797
AcyI GRCGYC 1 cut(s) 540
AfiI CCNNNNNNNGG 5 cut(s) 66, 209, 334, 986, 1093
AflIII ACRYGT 2 cut(s) 397, 1038
AgsI TTSAA 3 cut(s) 50, 427, 943
AjnI CCWGG 2 cut(s) 186, 681
AjuI GAANNNNNNNTTGG 2 cut(s) 1089, 1121
AluBI AGCT 8 cut(s) 206, 404, 557, 589, 875, 975, 1084, 1141
AluI AGCT 8 cut(s) 206, 404, 557, 589, 875, 975, 1084, 1141
Alw21I GWGCWC 1 cut(s) 511
Alw26I GTCTC 1 cut(s) 482
AlwI GGATC 1 cut(s) 745
AlwNI CAGNNNCTG 1 cut(s) 127
AoxI GGCC 4 cut(s) 19, 57, 388, 456
ApeKI GCWGC 6 cut(s) 92, 121, 158, 161, 302, 856
ApoI RAATTY 2 cut(s) 598, 797
AspS9I GGNCC 3 cut(s) 19, 1011, 1029
AsuHPI GGTGA 4 cut(s) 4, 241, 263, 926
AvaII GGWCC 2 cut(s) 1011, 1029
AxyI CCTNAGG 1 cut(s) 927
BalI TGGCCA 1 cut(s) 59
BanII GRGCYC 1 cut(s) 12
BarI GAAGNNNNNNTAC 2 cut(s) 1128, 1160
BbsI GAAGAC 1 cut(s) 1131
Bbv12I GWGCWC 1 cut(s) 511
BbvI GCAGC 6 cut(s) 79, 133, 145, 148, 314, 868
BccI CCATC 3 cut(s) 160, 185, 905
BceAI ACGGC 1 cut(s) 760
BciT130I CCWGG 2 cut(s) 188, 683
BclI TGATCA 1 cut(s) 1171
BcoDI GTCTC 1 cut(s) 482
BfmI CTRYAG 1 cut(s) 552
BisI GCNGC 6 cut(s) 93, 122, 159, 162, 303, 857
BlsI GCNGC 6 cut(s) 94, 123, 160, 163, 304, 858
Bme1390I CCNGG 2 cut(s) 188, 683
Bme18I GGWCC 2 cut(s) 1011, 1029
BmgT120I GGNCC 3 cut(s) 19, 1011, 1029
BmiI GGNNCC 1 cut(s) 1012
BmrFI CCNGG 2 cut(s) 188, 683
BpiI GAAGAC 1 cut(s) 1131
BpmI CTGGAG 1 cut(s) 525
Bpu10I CCTNAGC 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 1076
BsaHI GRCGYC 1 cut(s) 540
BsaJI CCNNGG 4 cut(s) 15, 391, 755, 1032
Bsc4I CCNNNNNNNGG 5 cut(s) 66, 209, 334, 986, 1093
Bse1I ACTGG 3 cut(s) 107, 641, 884
Bse21I CCTNAGG 1 cut(s) 927
Bse3DI GCAATG 3 cut(s) 160, 457, 770
BseBI CCWGG 2 cut(s) 188, 683
BseDI CCNNGG 4 cut(s) 15, 391, 755, 1032
BseGI GGATG 3 cut(s) 196, 706, 1050
BseLI CCNNNNNNNGG 5 cut(s) 66, 209, 334, 986, 1093
BseMI GCAATG 3 cut(s) 160, 457, 770
BseMII CTCAG 4 cut(s) 23, 532, 840, 941
BseNI ACTGG 3 cut(s) 107, 641, 884
BseRI GAGGAG 4 cut(s) 866, 915, 1018, 1166
BseXI GCAGC 6 cut(s) 79, 133, 145, 148, 314, 868
BseYI CCCAGC 1 cut(s) 74
BsgI GTGCAG 1 cut(s) 321
BshFI GGCC 4 cut(s) 21, 59, 390, 458
BsiHKAI GWGCWC 1 cut(s) 511
BsiSI CCGG 1 cut(s) 459
BslFI GGGAC 1 cut(s) 13
BslI CCNNNNNNNGG 5 cut(s) 66, 209, 334, 986, 1093
BsmAI GTCTC 1 cut(s) 482
BsmFI GGGAC 1 cut(s) 13
BsnI GGCC 4 cut(s) 21, 59, 390, 458
Bsp1286I GDGCHC 2 cut(s) 12, 511
Bsp143I GATC 4 cut(s) 43, 750, 953, 1171
Bsp19I CCATGG 1 cut(s) 391
BspACI CCGC 1 cut(s) 419
BspANI GGCC 4 cut(s) 21, 59, 390, 458
BspCNI CTCAG 4 cut(s) 24, 531, 841, 940
BspLI GGNNCC 1 cut(s) 1012
BspPI GGATC 1 cut(s) 745
BspQI GCTCTTC 4 cut(s) 648, 938, 965, 1131
BsrBI CCGCTC 1 cut(s) 419
BsrDI GCAATG 3 cut(s) 160, 457, 770
BsrI ACTGG 3 cut(s) 107, 641, 884
BssECI CCNNGG 4 cut(s) 15, 391, 755, 1032
BssMI GATC 4 cut(s) 43, 750, 953, 1171
BssNI GRCGYC 1 cut(s) 540
BssT1I CCWWGG 4 cut(s) 15, 391, 755, 1032
Bst2UI CCWGG 2 cut(s) 188, 683
Bst4CI ACNGT 5 cut(s) 703, 815, 840, 881, 986
Bst6I CTCTTC 6 cut(s) 648, 708, 938, 965, 1086, 1131
BstACI GRCGYC 1 cut(s) 540
BstAPI GCANNNNNTGC 1 cut(s) 130
BstC8I GCNNGC 2 cut(s) 90, 204
BstDEI CTNAG 4 cut(s) 32, 518, 849, 927
BstDSI CCRYGG 1 cut(s) 391
BstF5I GGATG 3 cut(s) 196, 706, 1050
BstKTI GATC 4 cut(s) 46, 753, 956, 1174
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 4 cut(s) 43, 750, 953, 1171
BstMWI GCNNNNNNNGC 5 cut(s) 130, 170, 212, 299, 539
BstNI CCWGG 2 cut(s) 188, 683
BstNSI RCATGY 3 cut(s) 92, 401, 1042
BstSCI CCNGG 2 cut(s) 186, 681
BstSFI CTRYAG 1 cut(s) 552
BstV1I GCAGC 6 cut(s) 79, 133, 145, 148, 314, 868
BstV2I GAAGAC 1 cut(s) 1131
BstX2I RGATCY 1 cut(s) 750
BstXI CCANNNNNNTGG 1 cut(s) 187
BstYI RGATCY 1 cut(s) 750
Bsu36I CCTNAGG 1 cut(s) 927
BsuRI GGCC 4 cut(s) 21, 59, 390, 458
BtgI CCRYGG 1 cut(s) 391
BtgZI GCGATG 1 cut(s) 179
BtsCI GGATG 3 cut(s) 196, 706, 1050
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 5 cut(s) 312, 526, 891, 956, 991
Cac8I GCNNGC 2 cut(s) 90, 204
CaiI CAGNNNCTG 1 cut(s) 127
Cfr13I GGNCC 3 cut(s) 19, 1011, 1029
CseI GACGC 1 cut(s) 548
CviAII CATG 9 cut(s) 25, 89, 392, 398, 446, 482, 544, 820, 1039
DdeI CTNAG 4 cut(s) 32, 518, 849, 927
DpnI GATC 4 cut(s) 45, 752, 955, 1173
DpnII GATC 4 cut(s) 43, 750, 953, 1171
EaeI YGGCCR 2 cut(s) 57, 388
Eam1104I CTCTTC 6 cut(s) 648, 708, 938, 965, 1086, 1131
EarI CTCTTC 6 cut(s) 648, 708, 938, 965, 1086, 1131
Eco130I CCWWGG 4 cut(s) 15, 391, 755, 1032
Eco24I GRGCYC 1 cut(s) 12
Eco32I GATATC 1 cut(s) 577
Eco47I GGWCC 2 cut(s) 1011, 1029
Eco81I CCTNAGG 1 cut(s) 927
EcoRII CCWGG 2 cut(s) 186, 681
EcoRV GATATC 1 cut(s) 577
EcoT14I CCWWGG 4 cut(s) 15, 391, 755, 1032
EcoT22I ATGCAT 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 12
ErhI CCWWGG 4 cut(s) 15, 391, 755, 1032
FaeI CATG 9 cut(s) 28, 92, 395, 401, 449, 485, 547, 823, 1042
FaqI GGGAC 1 cut(s) 13
FatI CATG 9 cut(s) 24, 88, 391, 397, 445, 481, 543, 819, 1038
FbaI TGATCA 1 cut(s) 1171
Fnu4HI GCNGC 6 cut(s) 93, 122, 159, 162, 303, 857
FokI GGATG 3 cut(s) 203, 693, 1037
FriOI GRGCYC 1 cut(s) 12
Fsp4HI GCNGC 6 cut(s) 93, 122, 159, 162, 303, 857
GluI GCNGC 6 cut(s) 93, 122, 159, 162, 303, 857
GsaI CCCAGC 1 cut(s) 78
GsuI CTGGAG 1 cut(s) 525
HaeIII GGCC 4 cut(s) 21, 59, 390, 458
HapII CCGG 1 cut(s) 459
HgaI GACGC 1 cut(s) 548
Hin1I GRCGYC 1 cut(s) 540
Hin1II CATG 9 cut(s) 28, 92, 395, 401, 449, 485, 547, 823, 1042
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HindIII AAGCTT 1 cut(s) 587
HinfI GANTC 4 cut(s) 310, 547, 607, 1187
HpaII CCGG 1 cut(s) 459
HphI GGTGA 4 cut(s) 4, 241, 263, 926
Hpy166II GTNNAC 3 cut(s) 646, 699, 904
Hpy188I TCNGA 5 cut(s) 612, 792, 804, 981, 1048
Hpy188III TCNNGA 5 cut(s) 47, 710, 764, 929, 1014
Hpy8I GTNNAC 3 cut(s) 646, 699, 904
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 1 cut(s) 338
HpyCH4III ACNGT 5 cut(s) 703, 815, 840, 881, 986
HpyCH4IV ACGT 1 cut(s) 1128
HpyCH4V TGCA 9 cut(s) 95, 138, 153, 215, 302, 353, 445, 569, 843
HpyF10VI GCNNNNNNNGC 5 cut(s) 130, 170, 212, 299, 539
HpyF3I CTNAG 4 cut(s) 32, 518, 849, 927
HpySE526I ACGT 1 cut(s) 1128
Hsp92I GRCGYC 1 cut(s) 540
Hsp92II CATG 9 cut(s) 28, 92, 395, 401, 449, 485, 547, 823, 1042
Ksp22I TGATCA 1 cut(s) 1171
Kzo9I GATC 4 cut(s) 43, 750, 953, 1171
LguI GCTCTTC 4 cut(s) 648, 938, 965, 1131
LmnI GCTCC 3 cut(s) 506, 853, 1089
Lsp1109I GCAGC 6 cut(s) 79, 133, 145, 148, 314, 868
MaeII ACGT 1 cut(s) 1128
MaeIII GTNAC 6 cut(s) 229, 307, 536, 736, 809, 815
MalI GATC 4 cut(s) 45, 752, 955, 1173
MbiI CCGCTC 1 cut(s) 419
MboI GATC 4 cut(s) 43, 750, 953, 1171
MfeI CAATTG 1 cut(s) 570
MflI RGATCY 1 cut(s) 750
MhlI GDGCHC 2 cut(s) 12, 511
MlsI TGGCCA 1 cut(s) 59
MluNI TGGCCA 1 cut(s) 59
MlyI GAGTC 3 cut(s) 304, 541, 1181
Mox20I TGGCCA 1 cut(s) 59
Mph1103I ATGCAT 1 cut(s) 447
MscI TGGCCA 1 cut(s) 59
MslI CAYNNNNRTG 3 cut(s) 396, 450, 1179
Msp20I TGGCCA 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 875
MspI CCGG 1 cut(s) 459
MspR9I CCNGG 2 cut(s) 188, 683
MunI CAATTG 1 cut(s) 570
MvaI CCWGG 2 cut(s) 188, 683
MwoI GCNNNNNNNGC 5 cut(s) 130, 170, 212, 299, 539
NcoI CCATGG 1 cut(s) 391
NdeII GATC 4 cut(s) 43, 750, 953, 1171
NlaIII CATG 9 cut(s) 28, 92, 395, 401, 449, 485, 547, 823, 1042
NlaIV GGNNCC 1 cut(s) 1012
NmeAIII GCCGAG 1 cut(s) 366
NmuCI GTSAC 6 cut(s) 229, 307, 536, 736, 809, 815
NsiI ATGCAT 1 cut(s) 447
NspI RCATGY 3 cut(s) 92, 401, 1042
PaeI GCATGC 1 cut(s) 92
PciI ACATGT 2 cut(s) 397, 1038
PciSI GCTCTTC 4 cut(s) 648, 938, 965, 1131
PfeI GAWTC 1 cut(s) 607
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 6 cut(s) 94, 123, 160, 163, 304, 858
PleI GAGTC 3 cut(s) 304, 541, 1181
PpsI GAGTC 3 cut(s) 304, 541, 1181
PscI ACATGT 2 cut(s) 397, 1038
PsiI TTATAA 2 cut(s) 833, 1115
Psp6I CCWGG 2 cut(s) 186, 681
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 2 cut(s) 186, 681
PspN4I GGNNCC 1 cut(s) 1012
PspPI GGNCC 3 cut(s) 19, 1011, 1029
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 1 cut(s) 750
PvuII CAGCTG 1 cut(s) 875
RseI CAYNNNNRTG 3 cut(s) 396, 450, 1179
SapI GCTCTTC 4 cut(s) 648, 938, 965, 1131
SatI GCNGC 6 cut(s) 93, 122, 159, 162, 303, 857
Sau3AI GATC 4 cut(s) 43, 750, 953, 1171
Sau96I GGNCC 3 cut(s) 19, 1011, 1029
SchI GAGTC 3 cut(s) 304, 541, 1181
ScrFI CCNGG 2 cut(s) 188, 683
SduI GDGCHC 2 cut(s) 12, 511
SfcI CTRYAG 1 cut(s) 552
SinI GGWCC 2 cut(s) 1011, 1029
SmiMI CAYNNNNRTG 3 cut(s) 396, 450, 1179
SmlI CTYRAG 1 cut(s) 1055
SmoI CTYRAG 1 cut(s) 1055
SphI GCATGC 1 cut(s) 92
SsiI CCGC 1 cut(s) 419
SspI AATATT 1 cut(s) 994
StyD4I CCNGG 2 cut(s) 186, 681
StyI CCWWGG 4 cut(s) 15, 391, 755, 1032
TaaI ACNGT 5 cut(s) 703, 815, 840, 881, 986
TaiI ACGT 1 cut(s) 1131
TaqI TCGA 5 cut(s) 42, 82, 513, 763, 1134
TfiI GAWTC 1 cut(s) 607
TscAI CASTG 5 cut(s) 312, 526, 891, 963, 991
TseFI GTSAC 6 cut(s) 229, 307, 536, 736, 809, 815
TseI GCWGC 6 cut(s) 92, 121, 158, 161, 302, 856
Tsp45I GTSAC 6 cut(s) 229, 307, 536, 736, 809, 815
TspDTI ATGAA 5 cut(s) 620, 666, 695, 810, 1179
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 5 cut(s) 312, 526, 891, 963, 991
VpaK11BI GGWCC 2 cut(s) 1011, 1029
XapI RAATTY 2 cut(s) 598, 797
XceI RCATGY 3 cut(s) 92, 401, 1042
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.