Rmu_sc0004795.1_g000020

phagosome-lysosome fusion involved in apoptotic cell clearance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004795.1
OG0003216
Physical Location & Seq
Reverse (-)
87126 .. 104186
17061 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 9000 bp
atggatttgtctgtgggtgataaaggacttgctacacttcagttgctcaaatggggcccttctcagccccaactaaacttgtcagaatttcgtgaagccttcatttcacctacaagacagttattattattgctttcgtatcagtgtgaggctttattgtttcctctaattacaggtagtaataatttgcagtgtaatattgatgaaagtcttcaaagtccagagacagagcctggtaggtcagattcaataagggacgatttgccatgcacatctggatcattaggagatgttgataatgatttgagttttggaggggacagtttaagatctaaaagttacccttttgttggtgatgttaactctctggcttggggcgtttgtgaggacacttataatcagcacaaggatgctttatttagagagcttttgtttgtgtgtggcaagcaaggtattatggtccatgcttttgttgaatctaccggaaatactgcaacaaccagcaatgcacgggaaggtgggtatgggcaagggaggtgggttgagtgggggccttccgcttctttaattggaaatatggaggtggaggaacctaccagcttgagttctgaagctactggaaatattgaatttaacaaatccaatggaaacagtgaaagtccccatatttgtaatgtggatgggaatgaggagttctcaaaaagtgttgcttctaaaagatggttgcaatcttttttgactaaagttgaaaatgttgaatataatggtaaaatattgaccaggttcccggaaaagtcattgttgcctagctctgcaagagttgtttcatttagtttatttaatagtaatttggcaattttggactgccttgcaaacaatgattctgcatcagataaggcgtgcggtcaggaaacagttcatgagttggacagtgataaatctctgaaattagacataacttcatctgatccacatttcaagtctgagactttgtccaatcttttaggtgttggaatgaatagtgtgtacaaatgctgcagagtgttctcaagcaattctcattacttcattggatttgttttcacacaggttgatccggtaattgtcaatactagtgatgaaagtggaaaaagcaagaagaataatgtactgcttattgctagattagaccacatgggaattcattgggtttcctcagtgaagcttgacgaaagtccaaatattggctcagtagcacaatggacagacttccacttttctgataaacttcttgtttgtcttaatgcttgtgggctaattgtattttatgcagcaatgtcaggtgagtatgttgcacatatagatattttacagactttaggactcaactctgagttgcatttgcagaagcaggaagcagtttccacaggttatgataagcatattactcaagttgatgacattcagaacaaatcagttttgcaacatgttgattattctggcaggagactgtttaaaaggttgatttctgcttcacatacctctctagtagctgctattgatgattatggtgtaatatatgtggtttctgctggtgaatttctaccggacaaatattatacaaatgcgaaactacttccacatattcagcatctggaacttggtatgttggctggttgggaggttggtggttctgatataagccaccagagggtatattctgacatatctggtttgtggaattctatcattccatccatgatgaaaggaagatccttcctggatggctctgggaaacaagtgcttcagagaaaccacgagttatacctcaaacaagaagggattggatacagatcagaagtaactgatcagaagcttgatgactctcacagaaagtcacaccttatgcgcaggatctttgttcccagttacagatttagtgagaaggatactatctgtttttctccattgggaattactcagcttatgaagaaccaaaatttgcacgatcagaggggttctcaaattgttcatctcaatctgcatgcagaatcagcagttcgtgatgacagctttttgaatactggttgtaaaaaggtttatctccacgggaaagaagaatcctttattgatgaggcagtgggttgcacattccagggatgcatttatttggttacagtaagtggactttcggtggtgcttccctctatagcagtttcatcaaattttcttcccgtagaagttattggatgtcggcagttgtgtgtcaactcaggcattggttatcaagtgaaagatataagggaagctaaagaatctaaacagccttggtcaccatggaatgttgaaattatggacagagttatcctatatgaaagcgctgaagaagccgatcgcttatgcttggagaatggttgggacctgaaaatatctcggatgcgtcggctgcagttggcactggattacttgaaatttgatgagatagagcgatcattagaaaaccttgtgggtgtcaatttggcagaagagggtgtactgagattgctatttgcggctgtttatctgatgcttcgtaaagttggcaatgataatgaggtttctgctgcatcaaggcttcttgcattagccactttttttgcgacgaaaatgattcgcaagtattggttgctagaacataggaaagatgcatatgaatctggtaggacccaaattctctctcttccaccagtttcaccggtgaaggaagaagatgaaatggcaaattcaaggaggcttcgtgagatggctcactttttggagataattcgaaatctgcaatctcgacttggttccaagtataagaggccaggccaagaatcggtggatagtggggaagcatcaagattggttgacactgatttattgcagaacgaatctcagctatcaattgtatctgtggacactatatctttggagacatcaaaacagcatgaagtttctttccctgtatcgacatcaggatttaactatactgataatcttgctttaacacctgtggattctaaagcccctttggacccagaggatttaagtgaagtatctgctcttgtgccacgagggggtctcttagagaagaaaattcttcccttagagaatccgaaagagatgattgctcgttggaagatagataatctagaccttagggctgtagtcagtgatgctttactctctggtcgtctgcccttagcagttcttcaactacatcttcaccgttcaagagattcattttctggaaaggagcctcatgatactttcactgaagtccgtgacgttggaagagctattgcttatgacctatttttgaagggtgaatctggacttgctgtggccacattgcaaaggctcggagaggatgtagaaaccagccttaagcagttattactcggcacagtgagacgatctcttcgagtgcaaatcactgaggagatgaataaatatggttatctgggatcttatgagtggaagatattggataggatatcacttattgagaggctataccccagcagtagtttttggaaaacacttcatggacggcagaaagaattcactcgaattccagcaagttccagtttgcccaagagatattatttgcgcctactggattcacctgtagtcaacaattttaccatcgagtgtgatgaaattgatggagttgtttttggttcatggacaaacgtaaatgaaaatccttctggtcctatggttgatgaagataatgcatacgctggttactgggctgctgctgctgtatggtttagtttctatgatcaaagaactgttgatcgaattgttttggatcagtcatctttcatgggtgtgcatgtattgtgggaatcacagcttgagtactatgcatgccacaatgactgggaggaagtctctagactcctggaccttattcctgaacatgttctagtagttggaagccttcaaatcaatttggatgggctacagcctgcttcaacttttgaatgcaatagaggttatgagtatggtgattacctctgctctgttgaagaattggatgctgtttgcatggatgtaccagagatcaaagttttcaggttttcgtgtaatgttatgtgctccatatggttaaagaggctgatggaagagaaacttgcgaggaaactaattttctcaaaggaatattgggaaggcacagcagatatactacctcttcttgcccgttctggcttcattactagcaagtatgagatcccttcagaggatgataatattgaggatatttcggtcctgaaatttccggatggtggcaccatacaggctttacataaactacttattcatcattgtgcacaatacaacttgccattccttctggacctttaccttgaccagcatgagttggtagcagatagtgattcagttcgttcacttcaggaagccgcaggagattgtcaatgggcacgatggttgctcctctctagggttaaagggtgtgagtatgaagcatcattttccaattctcgcgcgatactgtcacacaatctagttccggatagtaacctccgtgttccagagacggatgaaataattcgtactgttgatgacattgcagaaggagggggagaactggcagctctggcaaccttaatgtatgcctctgccccctttcaaagttgtctgagtagtggcagtgtaaagaggcatagtagtacatcagcccagtgcacactggagaatctcaggcccactcttcagcgttttcctacactgtggcacacatttgtatcagcatgtttcgggcaagatacaacttccaatcttgtggggcctaaagcaaagaatggtttttcagactatttaacatggcgtgatgacatattcttctcttctggacgtgatacttcacttctgcaaatgcttccatgctggtttccaaaggctgtgcggaggttaattcagctttatgctcagggtcctcttggatggcaatcaatagcaggtccgcctgtgggagaaggtctgctgcaaagagatatagaatttgtcctaaacactgatgaggatgtagaaatcagctcactctcctgggaagcaactatccaaaaacacattgaggaggaactctacaattctgcacttgagggaaatgcacttgggcttgaacaccatttacatcgtggacgtgcattagctgcttttaatcattttcttggttttagagttcaaaaattgaaatcagaaggaaaaggacaaatacaagcaaatgttcaagcagatgttcagacacttcttggacctataacagaaagtgaagagtctcttctttcatcagtcatgccacttgcaattatgcattttgaggattctgtgctggtgacttcatgcgcttttctactggagcttttcggattttctgccagcatgctccgcatagacattgctgccctgaaacggatgtcttgcttttacaaatctagtgacaatattgacaacttaaggaaactttcgacaaaagggtctgcatttcatgcagttggccatgaaagtgatataatggagtctctggctcgagctctagccgatgagtatctgcagcaggatagtgcaagcatgagtaagcaaaagggtactccaagtttagctgctcgtaaacaaccttcacgagctctcatgcttttcttagaattcctggagaaggctagccttcctccaatggcggatgggaaaacatgtgggtcttggctgttaagtggcgatggtgatggaattgagttgagatctcaacagaaagctgcaagccatcgctggaacttagttacaattttctgccagatgcaccaccttcccttgagcacaagatatctttctgtactggcaagagacaatgattgggttggctttttgtcagaagctcagattggtggatatccttttgacacggtggtccaagtggcgtcaaaggacttcagtgatccacgtcttaaaattcatatttcaacagttctgaaaggcatgcagtcaagaagaaaagctagctcttcaacaaatacagatactatggaaaaaaggagtggagcttcctttaccaatgaaagcatttgtgtcccagttgagctttttagaatattagcagaatgtgaaaagcagaaaaatcctggagaggccattttgatgaaagcaaaggagttatcctggtcaattttggccatgattgcatcatgcttttcagacgtgtctccaatatcttgcctaacagtctggctggaaataactgctgcaagggaaacttcatctatcaaggtgaatgatattgcttccagaattgcaaataatgttggagcagctgtggaagctactaatgctctgcaagcaggagggagtaaatctcttacatttcattacaataggcagaattcaaagaggaggcggcttctggaacccaactcaggagatccctcagctgctacagcatctgaaattttggattctcctgtgggtgcaaaaatatttgataaccagcgaactatttctgaaggggaaagaaatataaaacttggcggaaatatgaatttagctgattctgatgaagtgtctgtttcgctgtccaagatggtttcagtgctttgtgagcaacacctgttcctgcctttgttaagggcatttgaaatgttccttccttcatgttctcttgtacctttcatacgtgcacttcaggcattttcacaaatgcgcctttctgaagcatcagctcatttagattccttttgtgcccgaattaaagaagagtcgactcgcttacaggcaaatgtaggaagagatgtgcacattggtgcatcatggataagctctacggctattaaagctgcggatgctatgcttttgacatgtccatctccttatgaaaagagatgcttactaaaactccttgctgccactgattttggtgatggaggctccgcagctactcattaccagcggcttcattggaaaatcaatttagcggagccactattacgcaaggatgataatttgcacctagggaatgaaactctagatgatggtgcacttgcaacagcattggaaagtaatagacactgggagcaagcacgcaactgggcgagacagttggaggccagtgctggagtttggaaatccgctgttcatcatgtaactgaaactcaggctgaatctatggtagctgagtggaaggaatttctttgggatgttcccgaggagagaatcgctttatggggacattgccaaaccctcttcatcagatattccttccctgctttacaggctgggctatttttcctcaagcatgcagaagctctggaaaaagatcttccagctagggagcttcatgagctgttactgctttcactacaatggttgagcggaatgattactctgtccaatctagtttatccacttcatctgattcgggaaattgagactagagtatggctcttggcggtagaatcagaggcacaaggtaagagtgaaggagacttcaatctaagcagttccatccgtgatcctattcacaaaaatagctccagtattattgacagaacagcaagtatcataacaaaaatggacaatcacattggtacatttaagaacagaactgtagagaaacatgatgcaagggaaaataaccaggtgtatcacaagaaccaagtattagatgttagctttccaaccacaacagcagggagtacaaagactaagcgaagggcaaaaggctatgtgccattacgacggccggtactagactcaccagaaaaaagcggtgatcttgatgagggttctaattctctcaatgttagatatgaattgcagtctcaggatgaaaactcaaaaacggagatgtctttttcacggtgggaggaaagggttggacctgcagagctggaaagggctgttctttctttattagaatttggacaaatagctgctgcaaagcaactccagcataagctgtctccagtaaaagtaccatctgaaattttacttgtggacgccgcactgaagcttgctgctatgtcaactccaagtaaaaaagtatctatagcaatgcttgatgaagaagtacgttcagttattcagtcccaccatattctaacccagcaacatgaagttgatacagtgcaggttttggagagtctagcaaccatttttactgaaggtagcggacgtggactatgcaagaggataatagcagtcaacaaagcagcaagcatgttaggtcttccatttgcagaggcatttgttaagcagccaattgaactactacagctgctttcccttaaagcacaagaatcatttgaggaggcacatctccttgttagaactcattcaatgcccgcagccagtgttgctcagattctttccgaatcctttctaaagggcttattagctgctcatcgtgggggatatatggattctcaaaaagaagaaggacctgctcccttactctggaggttttcagacttcttaaagtgggcagagctttgtccttctgaacaagaaattgggcatgcactaatgcgcttggttattactggacaagaaataccacatgcctgcgaggttgagcttctaattttgtcacaccacttctacaaattatcatcttgccttgatggagttgatgtacttgtagcccttgcagctactagagttgaagcttatgtgtctgagggcgatttttcatgtttggctcgtctgataactggagtaggaaacttccacgcccttaatttcattctcggaattcttattgaaaatggccaactggatctccttcttcagaagtattcagctgctgcagatgcgaatgctggcactgctgaggccgtccgcggatttcggatggctgttctgacatcattgaagcattttaatcccaatgaccttgatgcttttgctatggttagtatctgcagacatgatttctcgaactatcaccaactatttcgcacagtttatgattattcaaattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spatacsin_C

Protein Analysis

2999

Amino Acids

334.29

Weight (kDa)

5.57

Isoelectric Point (pI)

49.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spatacsin_C PF14649 2843 - 2980 5.9e-31 Spatacsin C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 396
AasI GACNNNNNNGTC 2 cut(s) 5506, 5933
Acc16I TGCGCA 1 cut(s) 1889
Acc36I ACCTGC 4 cut(s) 4979, 7906, 8137, 8467
AccB1I GGYRCC 1 cut(s) 4298
AccB7I CCANNNNNTGG 1 cut(s) 1223
AccBSI CCGCTC 1 cut(s) 7389
AccI GTMKAC 1 cut(s) 6779
AccII CGCG 3 cut(s) 4515, 4517, 8859
AccIII TCCGGA 2 cut(s) 4288, 4540
AcoI YGGCCR 5 cut(s) 3345, 5527, 6195, 7757, 8785
AcyI GRCGYC 2 cut(s) 5945, 8016
AfeI AGCGCT 1 cut(s) 2359
AflII CTTAAG 2 cut(s) 3386, 5485
AflIII ACRYGT 5 cut(s) 1465, 3928, 5720, 6222, 6875
AgeI ACCGGT 1 cut(s) 2713
AhlI ACTAGT 1 cut(s) 1112
AjiI CACGTC 5 cut(s) 4886, 5174, 5969, 6223, 8192
AjuI GAANNNNNNNTTGG 4 cut(s) 5892, 5924, 8511, 8543
AleI CACNNNNGTG 1 cut(s) 6819
Alw21I GWGCWC 9 cut(s) 4109, 4342, 4718, 5566, 5659, 5846, 6700, 6816, 7057
Alw44I GTGCAC 5 cut(s) 4338, 4714, 6696, 6812, 7053
AlwNI CAGNNNCTG 4 cut(s) 233, 1633, 6464, 8822
Ama87I CYCGRG 2 cut(s) 5559, 7220
Aor13HI TCCGGA 2 cut(s) 4288, 4540
Aor51HI AGCGCT 1 cut(s) 2359
ApaI GGGCCC 1 cut(s) 59
ApaLI GTGCAC 5 cut(s) 4338, 4714, 6696, 6812, 7053
ArsI GACNNNNNNTTYG 2 cut(s) 3405, 3437
AsiGI ACCGGT 1 cut(s) 2713
Asp700I GAANNNNTTC 8 cut(s) 210, 915, 3563, 3930, 4578, 6517, 8350, 8394
AspA2I CCTAGG 1 cut(s) 7027
AspLEI GCGC 7 cut(s) 1890, 2360, 3615, 4517, 5378, 6723, 8547
AsuC2I CCSGG 1 cut(s) 788
AsuII TTCGAA 1 cut(s) 2785
AsuNHI GCTAGC 2 cut(s) 5690, 6023
AvaI CYCGRG 2 cut(s) 5559, 7220
AvrII CCTAGG 1 cut(s) 7027
AxyI CCTNAGG 1 cut(s) 3158
BaeGI GKGCMC 8 cut(s) 59, 4342, 4453, 4718, 6700, 6763, 6816, 7057
BaeI ACNNNNGTAYC 2 cut(s) 4758, 4791
BalI TGGCCA 4 cut(s) 3347, 5529, 6197, 8787
BanI GGYRCC 1 cut(s) 4298
BanII GRGCYC 3 cut(s) 59, 5566, 5659
BarI GAAGNNNNNNTAC 4 cut(s) 2496, 2528, 5632, 5664
BauI CACGAG 3 cut(s) 1796, 3072, 5652
BbsI GAAGAC 2 cut(s) 203, 8237
Bbv12I GWGCWC 9 cut(s) 4109, 4342, 4718, 5566, 5659, 5846, 6700, 6816, 7057
BbvCI CCTCAGC 2 cut(s) 6448, 8847
BceAI ACGGC 4 cut(s) 3569, 6858, 7772, 8837
BciVI GTATCC 2 cut(s) 1820, 1921
BclI TGATCA 2 cut(s) 1846, 3787
BcnI CCSGG 1 cut(s) 788
BcuI ACTAGT 1 cut(s) 1112
BfoI RGCGCY 1 cut(s) 2361
BfrI CTTAAG 2 cut(s) 3386, 5485
BfuAI ACCTGC 4 cut(s) 4979, 7906, 8137, 8467
BfuI GTATCC 2 cut(s) 1820, 1921
BglII AGATCT 3 cut(s) 329, 5768, 7333
BlnI CCTAGG 1 cut(s) 7027
BmcAI AGTACT 1 cut(s) 3869
BmeT110I CYCGRG 2 cut(s) 5559, 7220
BmgBI CACGTC 5 cut(s) 4886, 5174, 5969, 6223, 8192
BmrI ACTGGG 7 cut(s) 1899, 3763, 3898, 4705, 6092, 7096, 7114
BmtI GCTAGC 2 cut(s) 5694, 6027
BmuI ACTGGG 7 cut(s) 1899, 3763, 3898, 4705, 6092, 7096, 7114
BoxI GACNNNNGTC 1 cut(s) 1212
BpiI GAAGAC 2 cut(s) 203, 8237
Bpu10I CCTNAGC 4 cut(s) 3202, 4959, 6448, 8847
Bpu14I TTCGAA 1 cut(s) 2785
BpuEI CTTGAG 7 cut(s) 622, 1033, 1413, 3884, 5150, 5860, 7292
BpuMI CCSGG 1 cut(s) 788
BsaAI YACGTR 1 cut(s) 6695
BsaBI GATNNNNATC 3 cut(s) 1831, 4978, 5577
BsaHI GRCGYC 2 cut(s) 5945, 8016
BsaI GGTCTC 1 cut(s) 3086
BsaJI CCNNGG 8 cut(s) 2086, 2134, 2306, 2315, 5076, 7027, 7221, 8857
BsaWI WCCGGW 6 cut(s) 482, 1096, 1585, 2713, 4288, 4540
Bse118I RCCGGY 2 cut(s) 2713, 7759
Bse21I CCTNAGG 1 cut(s) 3158
Bse3DI GCAATG 8 cut(s) 511, 1320, 2569, 3350, 4596, 5427, 7246, 8076
Bse8I GATNNNNATC 3 cut(s) 1831, 4978, 5577
BseAI TCCGGA 2 cut(s) 4288, 4540
BseDI CCNNGG 8 cut(s) 2086, 2134, 2306, 2315, 5076, 7027, 7221, 8857
BseJI GATNNNNATC 3 cut(s) 1831, 4978, 5577
BseMI GCAATG 8 cut(s) 511, 1320, 2569, 3350, 4596, 5427, 7246, 8076
BseRI GAGGAG 7 cut(s) 704, 3455, 4454, 5120, 6427, 7238, 8339
BseSI GKGCMC 8 cut(s) 59, 4342, 4453, 4718, 6700, 6763, 6816, 7057
BseX3I CGGCCG 1 cut(s) 7757
BseYI CCCAGC 3 cut(s) 3521, 7292, 8121
BsgI GTGCAG 2 cut(s) 5109, 8165
Bsh1236I CGCG 3 cut(s) 4515, 4517, 8859
Bsh1285I CGRYCG 2 cut(s) 2374, 7760
BshNI GGYRCC 1 cut(s) 4298
BshTI ACCGGT 1 cut(s) 2713
BsiEI CGRYCG 2 cut(s) 2374, 7760
BsiHKAI GWGCWC 9 cut(s) 4109, 4342, 4718, 5566, 5659, 5846, 6700, 6816, 7057
BsiHKCI CYCGRG 2 cut(s) 5559, 7220
BsiSI CCGG 8 cut(s) 483, 788, 1097, 1586, 2714, 4289, 4541, 7760
BslFI GGGAC 7 cut(s) 269, 332, 645, 2411, 6080, 7257, 8089
BsmBI CGTCTC 2 cut(s) 3406, 4559
BsmFI GGGAC 7 cut(s) 269, 332, 645, 2411, 6080, 7257, 8089
BsmI GAATGC 2 cut(s) 3998, 8839
Bso31I GGTCTC 1 cut(s) 3086
BsoBI CYCGRG 2 cut(s) 5559, 7220
Bsp119I TTCGAA 1 cut(s) 2785
Bsp120I GGGCCC 1 cut(s) 55
Bsp13I TCCGGA 2 cut(s) 4288, 4540
Bsp1407I TGTACA 1 cut(s) 1026
Bsp19I CCATGG 1 cut(s) 2315
BspEI TCCGGA 2 cut(s) 4288, 4540
BspFNI CGCG 3 cut(s) 4515, 4517, 8859
BspHI TCATGA 3 cut(s) 919, 3262, 7354
BspMAI CTGCAG 6 cut(s) 1040, 2430, 5586, 7903, 8827, 8942
BspMI ACCTGC 4 cut(s) 4979, 7906, 8137, 8467
BspOI GCTAGC 2 cut(s) 5694, 6027
BspQI GCTCTTC 2 cut(s) 3289, 6034
BspT104I TTCGAA 1 cut(s) 2785
BspT107I GGYRCC 1 cut(s) 4298
BspTI CTTAAG 2 cut(s) 3386, 5485
BspTNI GGTCTC 1 cut(s) 3086
BsrBI CCGCTC 1 cut(s) 7389
BsrDI GCAATG 8 cut(s) 511, 1320, 2569, 3350, 4596, 5427, 7246, 8076
BsrFI RCCGGY 2 cut(s) 2713, 7759
BsrGI TGTACA 1 cut(s) 1026
BssAI RCCGGY 2 cut(s) 2713, 7759
BssECI CCNNGG 8 cut(s) 2086, 2134, 2306, 2315, 5076, 7027, 7221, 8857
BssNI GRCGYC 2 cut(s) 5945, 8016
BssSI CACGAG 3 cut(s) 1796, 3072, 5652
BssT1I CCWWGG 3 cut(s) 2306, 2315, 7027
Bst2BI CACGAG 3 cut(s) 1796, 3072, 5652
BstACI GRCGYC 2 cut(s) 5945, 8016
BstAFI CTTAAG 2 cut(s) 3386, 5485
BstAPI GCANNNNNTGC 2 cut(s) 5183, 5519
BstAUI TGTACA 1 cut(s) 1026
BstBAI YACGTR 1 cut(s) 6695
BstBI TTCGAA 1 cut(s) 2785
BstDSI CCRYGG 3 cut(s) 2086, 2315, 8857
BstEII GGTNACC 1 cut(s) 2310
BstENI CCTNNNNNAGG 1 cut(s) 5684
BstFNI CGCG 3 cut(s) 4515, 4517, 8859
BstH2I RGCGCY 1 cut(s) 2361
BstHHI GCGC 7 cut(s) 1890, 2360, 3615, 4517, 5378, 6723, 8547
BstMCI CGRYCG 2 cut(s) 2374, 7760
BstPAI GACNNNNGTC 1 cut(s) 1212
BstPI GGTNACC 1 cut(s) 2310
BstSLI GKGCMC 8 cut(s) 59, 4342, 4453, 4718, 6700, 6763, 6816, 7057
BstUI CGCG 3 cut(s) 4515, 4517, 8859
BstV2I GAAGAC 2 cut(s) 203, 8237
BstX2I RGATCY 9 cut(s) 329, 1751, 1893, 3467, 4239, 5768, 6442, 7333, 8794
BstXI CCANNNNNNTGG 1 cut(s) 4813
BstYI RGATCY 9 cut(s) 329, 1751, 1893, 3467, 4239, 5768, 6442, 7333, 8794
BstZI CGGCCG 1 cut(s) 7757
Bsu36I CCTNAGG 1 cut(s) 3158
BsuI GTATCC 2 cut(s) 1820, 1921
BtgI CCRYGG 3 cut(s) 2086, 2315, 8857
BtgZI GCGATG 2 cut(s) 5760, 5777
BtrI CACGTC 5 cut(s) 4886, 5174, 5969, 6223, 8192
BtsI GCAGTG 4 cut(s) 197, 2124, 4687, 8841
BveI ACCTGC 4 cut(s) 4979, 7906, 8137, 8467
CaiI CAGNNNCTG 4 cut(s) 233, 1633, 6464, 8822
CciI TCATGA 3 cut(s) 919, 3262, 7354
CfoI GCGC 7 cut(s) 1890, 2360, 3615, 4517, 5378, 6723, 8547
Cfr10I RCCGGY 2 cut(s) 2713, 7759
Cfr42I CCGCGG 1 cut(s) 8860
CseI GACGC 3 cut(s) 2408, 5934, 8024
CsiI ACCWGGT 2 cut(s) 779, 7653
CspAI ACCGGT 1 cut(s) 2713
CspCI CAANNNNNGTGG 6 cut(s) 518, 553, 2596, 2631, 6913, 6948
DraI TTTAAA 1 cut(s) 1495
DrdI GACNNNNNNGTC 2 cut(s) 5506, 5933
DseDI GACNNNNNNGTC 2 cut(s) 5506, 5933
EaeI YGGCCR 5 cut(s) 3345, 5527, 6195, 7757, 8785
EagI CGGCCG 1 cut(s) 7757
EciI GGCGGA 3 cut(s) 4983, 5723, 6564
Ecl136II GAGCTC 2 cut(s) 5564, 5657
EclXI CGGCCG 1 cut(s) 7757
Eco130I CCWWGG 3 cut(s) 2306, 2315, 7027
Eco24I GRGCYC 3 cut(s) 59, 5566, 5659
Eco31I GGTCTC 1 cut(s) 3086
Eco32I GATATC 3 cut(s) 3498, 5852, 5918
Eco47III AGCGCT 1 cut(s) 2359
Eco52I CGGCCG 1 cut(s) 7757
Eco53kI GAGCTC 2 cut(s) 5564, 5657
Eco81I CCTNAGG 1 cut(s) 3158
Eco88I CYCGRG 2 cut(s) 5559, 7220
Eco91I GGTNACC 1 cut(s) 2310
EcoICRI GAGCTC 2 cut(s) 5564, 5657
EcoNI CCTNNNNNAGG 1 cut(s) 5684
EcoO109I RGGNCCY 8 cut(s) 55, 56, 551, 2398, 2682, 4817, 4964, 8456
EcoO65I GGTNACC 1 cut(s) 2310
EcoRI GAATTC 7 cut(s) 1179, 1720, 3563, 3573, 5675, 6403, 8769
EcoRV GATATC 3 cut(s) 3498, 5852, 5918
EcoT14I CCWWGG 3 cut(s) 2306, 2315, 7027
EcoT22I ATGCAT 5 cut(s) 2144, 2668, 3742, 3877, 5346
EcoT38I GRGCYC 3 cut(s) 59, 5566, 5659
ErhI CCWWGG 3 cut(s) 2306, 2315, 7027
Esp3I CGTCTC 2 cut(s) 3406, 4559
FaqI GGGAC 7 cut(s) 269, 332, 645, 2411, 6080, 7257, 8089
FauI CCCGC 1 cut(s) 8368
FauNDI CATATG 2 cut(s) 2668, 4112
FbaI TGATCA 2 cut(s) 1846, 3787
FblI GTMKAC 1 cut(s) 6779
FriOI GRGCYC 3 cut(s) 59, 5566, 5659
FspI TGCGCA 1 cut(s) 1889
GlaI GCGC 7 cut(s) 1889, 2359, 3614, 4516, 5377, 6722, 8546
GsaI CCCAGC 3 cut(s) 3525, 7296, 8125
HaeII RGCGCY 1 cut(s) 2361
HapII CCGG 8 cut(s) 483, 788, 1097, 1586, 2714, 4289, 4541, 7760
HgaI GACGC 3 cut(s) 2408, 5934, 8024
HhaI GCGC 7 cut(s) 1890, 2360, 3615, 4517, 5378, 6723, 8547
Hin1I GRCGYC 2 cut(s) 5945, 8016
Hin6I GCGC 7 cut(s) 1888, 2358, 3613, 4515, 5376, 6721, 8545
HinP1I GCGC 7 cut(s) 1888, 2358, 3613, 4515, 5376, 6721, 8545
HincII GTYRAC 7 cut(s) 361, 2248, 2869, 3637, 6780, 8043, 8221
HindII GTYRAC 7 cut(s) 361, 2248, 2869, 3637, 6780, 8043, 8221
HindIII AAGCTT 4 cut(s) 1202, 1853, 8027, 8682
HpaI GTTAAC 1 cut(s) 361
HpaII CCGG 8 cut(s) 483, 788, 1097, 1586, 2714, 4289, 4541, 7760
Hpy99I CGWCG 3 cut(s) 2424, 2623, 7758
HpyCH4IV ACGT 9 cut(s) 3288, 3696, 4885, 5173, 5968, 6222, 6694, 8089, 8191
HpySE526I ACGT 9 cut(s) 3288, 3696, 4885, 5173, 5968, 6222, 6694, 8089, 8191
Hsp92I GRCGYC 2 cut(s) 5945, 8016
HspAI GCGC 7 cut(s) 1888, 2358, 3613, 4515, 5376, 6721, 8545
Kpn2I TCCGGA 2 cut(s) 4288, 4540
Ksp22I TGATCA 2 cut(s) 1846, 3787
KspAI GTTAAC 1 cut(s) 361
KspI CCGCGG 1 cut(s) 8860
LguI GCTCTTC 2 cut(s) 3289, 6034
MabI ACCWGGT 2 cut(s) 779, 7653
MaeII ACGT 9 cut(s) 3288, 3696, 4885, 5173, 5968, 6222, 6694, 8089, 8191
MbiI CCGCTC 1 cut(s) 7389
MfeI CAATTG 2 cut(s) 2904, 8277
MflI RGATCY 9 cut(s) 329, 1751, 1893, 3467, 4239, 5768, 6442, 7333, 8794
MlsI TGGCCA 4 cut(s) 3347, 5529, 6197, 8787
MluNI TGGCCA 4 cut(s) 3347, 5529, 6197, 8787
MlyI GAGTC 9 cut(s) 1356, 1856, 3900, 5315, 5558, 6775, 6785, 7763, 8167
MmeI TCCRAC 9 cut(s) 906, 991, 3116, 3271, 3922, 6307, 7098, 7718, 7873
Mox20I TGGCCA 4 cut(s) 3347, 5529, 6197, 8787
Mph1103I ATGCAT 5 cut(s) 2144, 2668, 3742, 3877, 5346
MroI TCCGGA 2 cut(s) 4288, 4540
MroXI GAANNNNTTC 8 cut(s) 210, 915, 3563, 3930, 4578, 6517, 8350, 8394
MscI TGGCCA 4 cut(s) 3347, 5529, 6197, 8787
MslI CAYNNNNRTG 7 cut(s) 408, 3836, 4335, 5535, 6161, 6819, 7378
Msp20I TGGCCA 4 cut(s) 3347, 5529, 6197, 8787
MspA1I CMGCKG 7 cut(s) 6335, 6452, 6967, 7148, 8293, 8819, 8859
MspCI CTTAAG 2 cut(s) 3386, 5485
MspI CCGG 8 cut(s) 483, 788, 1097, 1586, 2714, 4289, 4541, 7760
MunI CAATTG 2 cut(s) 2904, 8277
Mva1269I GAATGC 2 cut(s) 3998, 8839
MvnI CGCG 3 cut(s) 4515, 4517, 8859
NciI CCSGG 1 cut(s) 788
NcoI CCATGG 1 cut(s) 2315
NdeI CATATG 2 cut(s) 2668, 4112
NheI GCTAGC 2 cut(s) 5690, 6023
NmeAIII GCCGAG 1 cut(s) 3381
NmuCI GTSAC 7 cut(s) 1875, 2310, 3284, 4524, 5365, 5468, 8604
NsbI TGCGCA 1 cut(s) 1889
NsiI ATGCAT 5 cut(s) 2144, 2668, 3742, 3877, 5346
NspV TTCGAA 1 cut(s) 2785
OliI CACNNNNGTG 1 cut(s) 6819
PaeI GCATGC 6 cut(s) 2027, 3879, 5416, 6007, 7316, 8537
PaeR7I CTCGAG 1 cut(s) 5559
PagI TCATGA 3 cut(s) 919, 3262, 7354
PciI ACATGT 4 cut(s) 1465, 3928, 5720, 6875
PciSI GCTCTTC 2 cut(s) 3289, 6034
PcsI WCGNNNNNNNCGW 1 cut(s) 3421
PctI GAATGC 2 cut(s) 3998, 8839
PdmI GAANNNNTTC 8 cut(s) 210, 915, 3563, 3930, 4578, 6517, 8350, 8394
PflFI GACNNNGTC 1 cut(s) 991
PflMI CCANNNNNTGG 1 cut(s) 1223
PfoI TCCNGGA 5 cut(s) 786, 1758, 3909, 5679, 6145
PinAI ACCGGT 1 cut(s) 2713
Ple19I CGATCG 1 cut(s) 2374
PleI GAGTC 9 cut(s) 1356, 1856, 3900, 5314, 5557, 6775, 6784, 7763, 8166
PpsI GAGTC 9 cut(s) 1356, 1856, 3900, 5314, 5557, 6775, 6784, 7763, 8166
Ppu21I YACGTR 1 cut(s) 6695
PpuMI RGGWCCY 4 cut(s) 2398, 2682, 4964, 8456
PscI ACATGT 4 cut(s) 1465, 3928, 5720, 6875
PshAI GACNNNNGTC 1 cut(s) 1212
PsiI TTATAA 1 cut(s) 396
Psp124BI GAGCTC 2 cut(s) 5566, 5659
Psp5II RGGWCCY 4 cut(s) 2398, 2682, 4964, 8456
PspEI GGTNACC 1 cut(s) 2310
PspFI CCCAGC 3 cut(s) 3521, 7292, 8121
PspOMI GGGCCC 1 cut(s) 55
PspPPI RGGWCCY 4 cut(s) 2398, 2682, 4964, 8456
PspXI VCTCGAGB 1 cut(s) 5559
PsrI GAACNNNNNNTAC 2 cut(s) 5145, 5177
PstI CTGCAG 6 cut(s) 1040, 2430, 5586, 7903, 8827, 8942
PstNI CAGNNNCTG 4 cut(s) 233, 1633, 6464, 8822
PsuI RGATCY 9 cut(s) 329, 1751, 1893, 3467, 4239, 5768, 6442, 7333, 8794
PsyI GACNNNGTC 1 cut(s) 991
PvuI CGATCG 1 cut(s) 2374
PvuII CAGCTG 4 cut(s) 6335, 6452, 8293, 8819
RseI CAYNNNNRTG 7 cut(s) 408, 3836, 4335, 5535, 6161, 6819, 7378
SacI GAGCTC 2 cut(s) 5566, 5659
SacII CCGCGG 1 cut(s) 8860
SalI GTCGAC 1 cut(s) 6778
SapI GCTCTTC 2 cut(s) 3289, 6034
ScaI AGTACT 1 cut(s) 3869
SchI GAGTC 9 cut(s) 1356, 1856, 3900, 5315, 5558, 6775, 6785, 7763, 8167
SexAI ACCWGGT 2 cut(s) 779, 7653
Sfr274I CTCGAG 1 cut(s) 5559
Sfr303I CCGCGG 1 cut(s) 8860
SfuI TTCGAA 1 cut(s) 2785
SgrAI CRCCGGYG 1 cut(s) 2713
SgrBI CCGCGG 1 cut(s) 8860
SlaI CTCGAG 1 cut(s) 5559
SmiMI CAYNNNNRTG 7 cut(s) 408, 3836, 4335, 5535, 6161, 6819, 7378
SpeI ACTAGT 1 cut(s) 1112
SphI GCATGC 6 cut(s) 2027, 3879, 5416, 6007, 7316, 8537
SstI GAGCTC 2 cut(s) 5566, 5659
StyI CCWWGG 3 cut(s) 2306, 2315, 7027
TaiI ACGT 9 cut(s) 3291, 3699, 4888, 5176, 5971, 6225, 6697, 8092, 8194
TaqII GACCGA 1 cut(s) 4264
TatI WGTACW 8 cut(s) 1026, 1147, 2512, 3867, 4700, 5860, 7712, 8650
TauI GCSGC 5 cut(s) 2534, 4433, 6421, 6970, 8021
TseFI GTSAC 7 cut(s) 1875, 2310, 3284, 4524, 5365, 5468, 8604
Tsp45I GTSAC 7 cut(s) 1875, 2310, 3284, 4524, 5365, 5468, 8604
TspGWI ACGGA 6 cut(s) 3272, 4544, 4583, 5458, 7514, 7874
Tth111I GACNNNGTC 1 cut(s) 991
Van91I CCANNNNNTGG 1 cut(s) 1223
Vha464I CTTAAG 2 cut(s) 3386, 5485
VneI GTGCAC 5 cut(s) 4338, 4714, 6696, 6812, 7053
XagI CCTNNNNNAGG 1 cut(s) 5684
XbaI TCTAGA 3 cut(s) 3151, 3902, 7042
XcmI CCANNNNNNNNNTGG 1 cut(s) 5165
XhoI CTCGAG 1 cut(s) 5559
XmaJI CCTAGG 1 cut(s) 7027
XmiI GTMKAC 1 cut(s) 6779
XmnI GAANNNNTTC 8 cut(s) 210, 915, 3563, 3930, 4578, 6517, 8350, 8394
ZrmI AGTACT 1 cut(s) 3869
Zsp2I ATGCAT 5 cut(s) 2144, 2668, 3742, 3877, 5346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.