Rmu_sc0004805.1_g000050

SAC3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004805.1
OG0003610
Physical Location & Seq
Forward (+)
247748 .. 253275
5528 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1440 bp
atggagagaagaggccgtcgaaaccgttcttcttcttcttctcggggttcaagatcctggagcagaggtggaactagttccgccgagaaagtggaaaacgtcgccgtttccgatgttcccgccatagtgggtacttgcactttgatgtgcccagaagcagaaagggcacagcgggagcgccttcgcgatttagctacctttgagaggctgaatggaaatccagctcgctcatcgccatccattgcagttaaaaagtttttcagaactatgtccaccaaacaagttcaagcatcagatgttcggccgctccctgttttggaggatactttaaattacctcttagagttgtttgattctaaagactatccttttgaagtgattcacgacttcctctttgatcgaacaaggtccataaggcaagatcttagcatgcagaatattggtggcgagaaagcaattcacatgtatcagaaaaaggttaaatttcatatcatctctcttcataagcttagaggttgcagcagtcaaaatacttctccaatgcatcacctcaacatggagcagcttgcaaagaccttgacatctctatttaatctgtatggcgcaaatcgtcattccaattctactaatgaaaatgaggctgagttttgttctttttatgtgctacttcatcttggttctcatagccaacccctgggggagtcactttcttggtggtttagtcatgtgccttcttcgataatcaagtcaaaggaaatgcatttttcccgcaagattttacgatattttcggatgggtaactataaacgtttcttaagtactgtagcagctgaggcatcttatcttcaatactgcattattgagccttatgttgatgaggttcgagttttagctgtgtcatgcatcaatagtggtggctacaagcttcatccatatcctctgtcgcatttatccaaactcttgattatgaaggaatcagatttggaatcttttctcaaatcctgtggtcttcaaatttgcactgatgaagaaggacataatctgttgcccactaagcaaacgaccttttgtcaacccaaggatgggtctcaaaactacaggttttgggatggttggaatggcttacctgttggtccggccgccgaaggttcaggtggtcaggtagatgtggagggttggttgctctgctggtctaaccagaagccggatgctgcagatcagaggatgggacatccagttagggccgttgggtttggggtcgagtctaggttatacttcaggttgaatttgttgatccaattgaaagtcaattcagcagctgggtttgtgtctttgggcctggttgtcttcagcacacgatggaaggtgttttaccaccctcatttcttacttaacattgggcaggtgaggttgggggcttttacactatggcgttggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAC3_GANP

Protein Analysis

479

Amino Acids

54.48

Weight (kDa)

9.29

Isoelectric Point (pI)

51.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 49 - 339 4.5e-79 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1393
Acc36I ACCTGC 1 cut(s) 1393
AccB7I CCANNNNNTGG 1 cut(s) 694
AccBSI CCGCTC 1 cut(s) 307
AccII CGCG 1 cut(s) 186
AciI CCGC 6 cut(s) 81, 120, 172, 305, 769, 1140
AclI AACGTT 1 cut(s) 808
AclWI GGATC 2 cut(s) 48, 1288
AcoI YGGCCR 2 cut(s) 302, 1137
AcsI RAATTY 3 cut(s) 482, 1014, 1285
AcuI CTGAAG 2 cut(s) 1261, 1333
AfaI GTAC 2 cut(s) 133, 820
AfiI CCNNNNNNNGG 7 cut(s) 204, 316, 556, 694, 1082, 1083, 1204
AflII CTTAAG 1 cut(s) 814
AflIII ACRYGT 1 cut(s) 462
AgsI TTSAA 7 cut(s) 51, 287, 374, 848, 1013, 1285, 1303
AhdI GACNNNNNGTC 1 cut(s) 1068
AhlI ACTAGT 1 cut(s) 74
AjnI CCWGG 3 cut(s) 56, 693, 1338
AluBI AGCT 8 cut(s) 194, 224, 508, 565, 830, 893, 925, 1319
AluI AGCT 8 cut(s) 194, 224, 508, 565, 830, 893, 925, 1319
Alw26I GTCTC 1 cut(s) 1092
AlwI GGATC 2 cut(s) 48, 1288
AlwNI CAGNNNCTG 1 cut(s) 1319
Ama87I CYCGRG 1 cut(s) 42
AoxI GGCC 5 cut(s) 13, 302, 1137, 1242, 1336
ApeKI GCWGC 5 cut(s) 519, 562, 827, 1211, 1316
ApoI RAATTY 3 cut(s) 482, 1014, 1285
ArsI GACNNNNNNTTYG 4 cut(s) 353, 377, 385, 409
Asp700I GAANNNNTTC 4 cut(s) 25, 76, 378, 990
AspLEI GCGC 2 cut(s) 180, 605
AspS9I GGNCC 4 cut(s) 408, 1133, 1242, 1336
AsuHPI GGTGA 2 cut(s) 539, 1417
AvaI CYCGRG 1 cut(s) 42
AvaII GGWCC 2 cut(s) 408, 1133
BaeGI GKGCMC 2 cut(s) 152, 169
BarI GAAGNNNNNNTAC 2 cut(s) 1355, 1387
BbsI GAAGAC 2 cut(s) 1001, 1339
BbvCI CCTCAGC 1 cut(s) 831
BbvI GCAGC 5 cut(s) 531, 574, 839, 1198, 1328
BccI CCATC 6 cut(s) 244, 787, 1076, 1103, 1219, 1353
BceAI ACGGC 2 cut(s) 89, 1229
BciT130I CCWGG 3 cut(s) 58, 695, 1340
BciVI GTATCC 1 cut(s) 316
BcoDI GTCTC 1 cut(s) 1092
BcuI ACTAGT 1 cut(s) 74
BfaI CTAG 2 cut(s) 75, 1266
BfmI CTRYAG 3 cut(s) 822, 1096, 1212
BfoI RGCGCY 1 cut(s) 181
BfrI CTTAAG 1 cut(s) 814
BfuAI ACCTGC 1 cut(s) 1393
BfuI GTATCC 1 cut(s) 316
BglII AGATCT 1 cut(s) 421
BisI GCNGC 7 cut(s) 305, 520, 563, 828, 1140, 1212, 1317
BlsI GCNGC 7 cut(s) 306, 521, 564, 829, 1141, 1213, 1318
BmcAI AGTACT 1 cut(s) 820
Bme1390I CCNGG 3 cut(s) 58, 695, 1340
Bme18I GGWCC 2 cut(s) 408, 1133
BmeRI GACNNNNNGTC 1 cut(s) 1068
BmeT110I CYCGRG 1 cut(s) 42
BmgT120I GGNCC 4 cut(s) 408, 1133, 1242, 1336
BmrFI CCNGG 3 cut(s) 58, 695, 1340
BmsI GCATC 5 cut(s) 299, 553, 845, 912, 1198
BpiI GAAGAC 2 cut(s) 1001, 1339
BpmI CTGGAG 1 cut(s) 79
Bpu10I CCTNAGC 1 cut(s) 831
BsaI GGTCTC 1 cut(s) 1092
BsaJI CCNNGG 3 cut(s) 693, 694, 1077
BsaXI ACNNNNNCTCC 4 cut(s) 52, 82, 1163, 1193
Bsc4I CCNNNNNNNGG 7 cut(s) 204, 316, 556, 694, 1082, 1083, 1204
Bse1I ACTGG 1 cut(s) 1235
Bse3DI GCAATG 1 cut(s) 240
BseBI CCWGG 3 cut(s) 58, 695, 1340
BseDI CCNNGG 3 cut(s) 693, 694, 1077
BseGI GGATG 8 cut(s) 236, 798, 928, 1087, 1114, 1213, 1230, 1231
BseLI CCNNNNNNNGG 7 cut(s) 204, 316, 556, 694, 1082, 1083, 1204
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 2 cut(s) 633, 822
BseNI ACTGG 1 cut(s) 1235
BseSI GKGCMC 2 cut(s) 152, 169
BseX3I CGGCCG 2 cut(s) 302, 1137
BseXI GCAGC 5 cut(s) 531, 574, 839, 1198, 1328
BseYI CCCAGC 1 cut(s) 1319
Bsh1236I CGCG 1 cut(s) 186
Bsh1285I CGRYCG 2 cut(s) 305, 1140
BshFI GGCC 5 cut(s) 15, 304, 1139, 1244, 1338
BsiEI CGRYCG 2 cut(s) 305, 1140
BsiHKCI CYCGRG 1 cut(s) 42
BsiSI CCGG 2 cut(s) 1136, 1205
BslFI GGGAC 1 cut(s) 1242
BslI CCNNNNNNNGG 7 cut(s) 204, 316, 556, 694, 1082, 1083, 1204
BsmAI GTCTC 1 cut(s) 1092
BsmFI GGGAC 1 cut(s) 1242
BsnI GGCC 5 cut(s) 15, 304, 1139, 1244, 1338
Bso31I GGTCTC 1 cut(s) 1092
BsoBI CYCGRG 1 cut(s) 42
Bsp1286I GDGCHC 2 cut(s) 152, 169
Bsp143I GATC 5 cut(s) 53, 397, 421, 1216, 1293
Bsp68I TCGCGA 1 cut(s) 186
BspACI CCGC 6 cut(s) 81, 120, 172, 305, 769, 1140
BspANI GGCC 5 cut(s) 15, 304, 1139, 1244, 1338
BspCNI CTCAG 2 cut(s) 634, 823
BspFNI CGCG 1 cut(s) 186
BspMAI CTGCAG 1 cut(s) 1216
BspMI ACCTGC 1 cut(s) 1393
BspPI GGATC 2 cut(s) 48, 1288
BspTI CTTAAG 1 cut(s) 814
BspTNI GGTCTC 1 cut(s) 1092
BsrBI CCGCTC 1 cut(s) 307
BsrDI GCAATG 1 cut(s) 240
BsrI ACTGG 1 cut(s) 1235
BssECI CCNNGG 3 cut(s) 693, 694, 1077
BssMI GATC 5 cut(s) 53, 397, 421, 1216, 1293
BssT1I CCWWGG 1 cut(s) 1077
Bst2UI CCWGG 3 cut(s) 58, 695, 1340
Bst4CI ACNGT 2 cut(s) 26, 823
Bst6I CTCTTC 2 cut(s) 4, 504
BstAFI CTTAAG 1 cut(s) 814
BstC8I GCNNGC 3 cut(s) 226, 431, 567
BstDEI CTNAG 6 cut(s) 340, 425, 509, 642, 831, 1053
BstENI CCTNNNNNAGG 1 cut(s) 202
BstF5I GGATG 8 cut(s) 236, 798, 928, 1087, 1114, 1213, 1230, 1231
BstFNI CGCG 1 cut(s) 186
BstH2I RGCGCY 1 cut(s) 181
BstHHI GCGC 2 cut(s) 180, 605
BstKTI GATC 5 cut(s) 56, 400, 424, 1219, 1296
BstMAI GTCTC 1 cut(s) 1092
BstMBI GATC 5 cut(s) 53, 397, 421, 1216, 1293
BstMCI CGRYCG 2 cut(s) 305, 1140
BstMWI GCNNNNNNNGC 3 cut(s) 164, 833, 1054
BstNI CCWGG 3 cut(s) 58, 695, 1340
BstNSI RCATGY 2 cut(s) 433, 466
BstSCI CCNGG 3 cut(s) 56, 693, 1338
BstSFI CTRYAG 3 cut(s) 822, 1096, 1212
BstSLI GKGCMC 2 cut(s) 152, 169
BstUI CGCG 1 cut(s) 186
BstV1I GCAGC 5 cut(s) 531, 574, 839, 1198, 1328
BstV2I GAAGAC 2 cut(s) 1001, 1339
BstX2I RGATCY 2 cut(s) 53, 421
BstYI RGATCY 2 cut(s) 53, 421
BstZI CGGCCG 2 cut(s) 302, 1137
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 5 cut(s) 15, 304, 1139, 1244, 1338
BtgZI GCGATG 1 cut(s) 216
BtsCI GGATG 8 cut(s) 236, 798, 928, 1087, 1114, 1213, 1230, 1231
BtsIMutI CAGTG 1 cut(s) 1020
BtuMI TCGCGA 1 cut(s) 186
BveI ACCTGC 1 cut(s) 1393
Cac8I GCNNGC 3 cut(s) 226, 431, 567
CaiI CAGNNNCTG 1 cut(s) 1319
CfoI GCGC 2 cut(s) 180, 605
Cfr13I GGNCC 4 cut(s) 408, 1133, 1242, 1336
Csp6I GTAC 2 cut(s) 132, 819
CspCI CAANNNNNGTGG 4 cut(s) 895, 930, 985, 1020
CviAII CATG 5 cut(s) 430, 463, 556, 725, 900
CviQI GTAC 2 cut(s) 132, 819
DdeI CTNAG 6 cut(s) 340, 425, 509, 642, 831, 1053
DpnI GATC 5 cut(s) 55, 399, 423, 1218, 1295
DpnII GATC 5 cut(s) 53, 397, 421, 1216, 1293
DraI TTTAAA 1 cut(s) 330
DriI GACNNNNNGTC 1 cut(s) 1068
EaeI YGGCCR 2 cut(s) 302, 1137
EagI CGGCCG 2 cut(s) 302, 1137
Eam1104I CTCTTC 2 cut(s) 4, 504
Eam1105I GACNNNNNGTC 1 cut(s) 1068
EarI CTCTTC 2 cut(s) 4, 504
EciI GGCGGA 1 cut(s) 70
EclXI CGGCCG 2 cut(s) 302, 1137
Eco130I CCWWGG 1 cut(s) 1077
Eco31I GGTCTC 1 cut(s) 1092
Eco47I GGWCC 2 cut(s) 408, 1133
Eco52I CGGCCG 2 cut(s) 302, 1137
Eco57I CTGAAG 2 cut(s) 1261, 1333
Eco88I CYCGRG 1 cut(s) 42
EcoNI CCTNNNNNAGG 1 cut(s) 202
EcoRII CCWGG 3 cut(s) 56, 693, 1338
EcoT14I CCWWGG 1 cut(s) 1077
EcoT22I ATGCAT 3 cut(s) 546, 762, 905
ErhI CCWWGG 1 cut(s) 1077
FaeI CATG 5 cut(s) 433, 466, 559, 728, 903
FaqI GGGAC 1 cut(s) 1242
FatI CATG 5 cut(s) 429, 462, 555, 724, 899
FauI CCCGC 3 cut(s) 127, 165, 776
Fnu4HI GCNGC 7 cut(s) 305, 520, 563, 828, 1140, 1212, 1317
FokI GGATG 8 cut(s) 223, 805, 915, 1094, 1121, 1218, 1220, 1237
Fsp4HI GCNGC 7 cut(s) 305, 520, 563, 828, 1140, 1212, 1317
FspBI CTAG 2 cut(s) 75, 1266
GlaI GCGC 2 cut(s) 179, 604
GluI GCNGC 7 cut(s) 305, 520, 563, 828, 1140, 1212, 1317
GsaI CCCAGC 1 cut(s) 1323
GsuI CTGGAG 1 cut(s) 79
HaeII RGCGCY 1 cut(s) 181
HaeIII GGCC 5 cut(s) 15, 304, 1139, 1244, 1338
HapII CCGG 2 cut(s) 1136, 1205
HhaI GCGC 2 cut(s) 180, 605
Hin1II CATG 5 cut(s) 433, 466, 559, 728, 903
Hin6I GCGC 2 cut(s) 178, 603
HinP1I GCGC 2 cut(s) 178, 603
HincII GTYRAC 1 cut(s) 1073
HindII GTYRAC 1 cut(s) 1073
HindIII AAGCTT 2 cut(s) 506, 923
HinfI GANTC 6 cut(s) 353, 379, 701, 974, 986, 1262
HpaII CCGG 2 cut(s) 1136, 1205
HphI GGTGA 2 cut(s) 539, 1417
Hpy166II GTNNAC 2 cut(s) 273, 1073
Hpy188I TCNGA 7 cut(s) 112, 263, 295, 471, 792, 979, 1221
Hpy188III TCNNGA 4 cut(s) 51, 185, 383, 961
Hpy8I GTNNAC 2 cut(s) 273, 1073
Hpy99I CGWCG 2 cut(s) 21, 104
HpyAV CCTTC 6 cut(s) 191, 741, 964, 1025, 1139, 1357
HpyCH4III ACNGT 2 cut(s) 26, 823
HpyCH4IV ACGT 2 cut(s) 99, 808
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 833, 1054
HpyF3I CTNAG 6 cut(s) 340, 425, 509, 642, 831, 1053
HpySE526I ACGT 2 cut(s) 99, 808
Hsp92II CATG 5 cut(s) 433, 466, 559, 728, 903
HspAI GCGC 2 cut(s) 178, 603
Kzo9I GATC 5 cut(s) 53, 397, 421, 1216, 1293
LmnI GCTCC 4 cut(s) 60, 175, 312, 559
Lsp1109I GCAGC 5 cut(s) 531, 574, 839, 1198, 1328
LweI GCATC 5 cut(s) 299, 553, 845, 912, 1198
MaeI CTAG 2 cut(s) 75, 1266
MaeII ACGT 2 cut(s) 99, 808
MaeIII GTNAC 2 cut(s) 702, 797
MalI GATC 5 cut(s) 55, 399, 423, 1218, 1295
MbiI CCGCTC 1 cut(s) 307
MboI GATC 5 cut(s) 53, 397, 421, 1216, 1293
MfeI CAATTG 1 cut(s) 1298
MflI RGATCY 2 cut(s) 53, 421
MhlI GDGCHC 2 cut(s) 152, 169
MluCI AATT 8 cut(s) 331, 456, 482, 619, 1014, 1285, 1298, 1309
MlyI GAGTC 2 cut(s) 710, 1271
MmeI TCCRAC 1 cut(s) 1094
Mph1103I ATGCAT 3 cut(s) 546, 762, 905
MroXI GAANNNNTTC 4 cut(s) 25, 76, 378, 990
MseI TTAA 6 cut(s) 249, 329, 480, 591, 815, 1392
MslI CAYNNNNRTG 1 cut(s) 143
MspA1I CMGCKG 3 cut(s) 172, 830, 1319
MspCI CTTAAG 1 cut(s) 814
MspI CCGG 2 cut(s) 1136, 1205
MspR9I CCNGG 3 cut(s) 58, 695, 1340
MunI CAATTG 1 cut(s) 1298
MvaI CCWGG 3 cut(s) 58, 695, 1340
MvnI CGCG 1 cut(s) 186
MwoI GCNNNNNNNGC 3 cut(s) 164, 833, 1054
NdeII GATC 5 cut(s) 53, 397, 421, 1216, 1293
NlaIII CATG 5 cut(s) 433, 466, 559, 728, 903
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 1 cut(s) 702
NruI TCGCGA 1 cut(s) 186
NsiI ATGCAT 3 cut(s) 546, 762, 905
NspI RCATGY 2 cut(s) 433, 466
PaeI GCATGC 1 cut(s) 433
PaqCI CACCTGC 1 cut(s) 1393
PasI CCCWGGG 1 cut(s) 694
PciI ACATGT 1 cut(s) 462
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdmI GAANNNNTTC 4 cut(s) 25, 76, 378, 990
PfeI GAWTC 4 cut(s) 353, 379, 974, 986
PflMI CCANNNNNTGG 1 cut(s) 694
PfoI TCCNGGA 1 cut(s) 56
PkrI GCNGC 7 cut(s) 306, 521, 564, 829, 1141, 1213, 1318
PleI GAGTC 2 cut(s) 709, 1270
PpsI GAGTC 2 cut(s) 709, 1270
PscI ACATGT 1 cut(s) 462
Psp1406I AACGTT 1 cut(s) 808
Psp6I CCWGG 3 cut(s) 56, 693, 1338
PspFI CCCAGC 1 cut(s) 1319
PspGI CCWGG 3 cut(s) 56, 693, 1338
PspPI GGNCC 4 cut(s) 408, 1133, 1242, 1336
PstI CTGCAG 1 cut(s) 1216
PstNI CAGNNNCTG 1 cut(s) 1319
PsuI RGATCY 2 cut(s) 53, 421
PvuII CAGCTG 2 cut(s) 830, 1319
RruI TCGCGA 1 cut(s) 186
RsaI GTAC 2 cut(s) 133, 820
RsaNI GTAC 2 cut(s) 132, 819
RseI CAYNNNNRTG 1 cut(s) 143
SaqAI TTAA 6 cut(s) 249, 329, 480, 591, 815, 1392
SatI GCNGC 7 cut(s) 305, 520, 563, 828, 1140, 1212, 1317
Sau3AI GATC 5 cut(s) 53, 397, 421, 1216, 1293
Sau96I GGNCC 4 cut(s) 408, 1133, 1242, 1336
ScaI AGTACT 1 cut(s) 820
SchI GAGTC 2 cut(s) 710, 1271
ScrFI CCNGG 3 cut(s) 58, 695, 1340
SduI GDGCHC 2 cut(s) 152, 169
SfaNI GCATC 5 cut(s) 299, 553, 845, 912, 1198
SfcI CTRYAG 3 cut(s) 822, 1096, 1212
SinI GGWCC 2 cut(s) 408, 1133
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 814
SmoI CTYRAG 1 cut(s) 814
SpeI ACTAGT 1 cut(s) 74
SphI GCATGC 1 cut(s) 433
Sse9I AATT 8 cut(s) 331, 456, 482, 619, 1014, 1285, 1298, 1309
SsiI CCGC 6 cut(s) 81, 120, 172, 305, 769, 1140
SspI AATATT 1 cut(s) 439
SspMI CTAG 2 cut(s) 75, 1266
StyD4I CCNGG 3 cut(s) 56, 693, 1338
StyI CCWWGG 1 cut(s) 1077
TaaI ACNGT 2 cut(s) 26, 823
TaiI ACGT 2 cut(s) 102, 811
TaqI TCGA 5 cut(s) 19, 400, 737, 883, 1260
TasI AATT 8 cut(s) 331, 456, 482, 619, 1014, 1285, 1298, 1309
TatI WGTACW 1 cut(s) 818
TauI GCSGC 2 cut(s) 307, 1142
TfiI GAWTC 4 cut(s) 353, 379, 974, 986
Tru1I TTAA 6 cut(s) 249, 329, 480, 591, 815, 1392
Tru9I TTAA 6 cut(s) 249, 329, 480, 591, 815, 1392
TscAI CASTG 1 cut(s) 1027
TseFI GTSAC 1 cut(s) 702
TseI GCWGC 5 cut(s) 519, 562, 827, 1211, 1316
Tsp45I GTSAC 1 cut(s) 702
TspDTI ATGAA 7 cut(s) 476, 491, 645, 659, 917, 983, 1041
TspRI CASTG 1 cut(s) 1027
Van91I CCANNNNNTGG 1 cut(s) 694
Vha464I CTTAAG 1 cut(s) 814
VpaK11BI GGWCC 2 cut(s) 408, 1133
XagI CCTNNNNNAGG 1 cut(s) 202
XapI RAATTY 3 cut(s) 482, 1014, 1285
XceI RCATGY 2 cut(s) 433, 466
XmnI GAANNNNTTC 4 cut(s) 25, 76, 378, 990
XspI CTAG 2 cut(s) 75, 1266
ZrmI AGTACT 1 cut(s) 820
Zsp2I ATGCAT 3 cut(s) 546, 762, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.