Rmu_sc0005625.1_g000009

2-aminoethanethiol dioxygenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005625.1
OG0004451
Physical Location & Seq
Reverse (-)
36000 .. 45263
9264 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1017 bp
atgatctatgagatctctggaaacttctttggggacgttcggttaggttttcagagaagtctggcagctggcggttggtgttcggattccggtgacaagagtcccgcgtttgttcctcttcttattttgctaccgatttctcacttcatcgcctacctcccgcttcgttttccacctaacccttcatggctctcaacccaggtgctcacagatgagattggcgagagggaacaaataagtgccatgcagcttcattggctctggtccattcagttaagttggtaccgtctttgcatcatgccatattatattcagagactctataatctgtgcaaatcatctctttcgcccaatggacctctttccgaagaagccctcgaaaaagttcgcactaaactagagaaaattaagcctgcagatgtaggtctagaacaagaggcacaagtggtacgcaactggtcaggtgctatgcttgagcgtaatgtggatcatcagtcactgccaccaatcaaatacttgcatttacatgaatgcgatagtttctctatgggaatcttttgcatgccgccttcctctatcatacctcttcataatcatccaggaatgactgtgttgagcaagcttatatatggtactttgcgtgttaattcatatgactggcttgatttacccgggtctgctgatccatcagaagcaagaccggcgaaacttgtcagagatgatgagatgaccgctccttgcccggcaacagttctctatcccacaagtggaggcaacatccatagtttcaaagctataactccctgtgccatatttgacattctagctccaccttattcgtctgaagatggtcgacactgcacttatttccggaagtcctcaagaaaagatctgcctggtgatgttgagtatgatggagtcacagctcctcaagtgacttggttagaagagtttcaacctcccgacaactttgtgattcggagagggctctacaggggtcctgttattcgaacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00430 ko01100 map00430 map01100
Pfam Domains
Protein Families
PCO_ADO

Protein Analysis

338

Amino Acids

38.13

Weight (kDa)

6.0

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 133 - 336 3e-75 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 282
AccBSI CCGCTC 1 cut(s) 734
AccI GTMKAC 1 cut(s) 853
AccII CGCG 1 cut(s) 107
AccIII TCCGGA 1 cut(s) 870
AciI CCGC 5 cut(s) 72, 105, 161, 566, 732
AclWI GGATC 2 cut(s) 495, 677
AcuI CTGAAG 1 cut(s) 864
AfaI GTAC 3 cut(s) 284, 450, 634
AfiI CCNNNNNNNGG 1 cut(s) 767
AgsI TTSAA 2 cut(s) 790, 956
AjnI CCWGG 3 cut(s) 198, 598, 895
AluBI AGCT 6 cut(s) 68, 250, 622, 794, 827, 926
AluI AGCT 6 cut(s) 68, 250, 622, 794, 827, 926
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 1 cut(s) 310
AlwI GGATC 2 cut(s) 495, 677
AlwNI CAGNNNCTG 1 cut(s) 499
Ama87I CYCGRG 1 cut(s) 671
Aor13HI TCCGGA 1 cut(s) 870
ApeKI GCWGC 2 cut(s) 65, 247
Asp700I GAANNNNTTC 2 cut(s) 384, 951
Asp718I GGTACC 1 cut(s) 282
AspS9I GGNCC 3 cut(s) 264, 356, 998
AsuC2I CCSGG 3 cut(s) 672, 673, 743
AsuHPI GGTGA 2 cut(s) 104, 911
AsuII TTCGAA 1 cut(s) 1009
AvaI CYCGRG 1 cut(s) 671
AvaII GGWCC 3 cut(s) 264, 356, 998
BanI GGYRCC 1 cut(s) 282
BanII GRGCYC 1 cut(s) 990
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 2 cut(s) 77, 259
BccI CCATC 3 cut(s) 694, 842, 908
BciT130I CCWGG 3 cut(s) 200, 600, 897
BcnI CCSGG 3 cut(s) 672, 673, 743
BcoDI GTCTC 1 cut(s) 310
BfaI CTAG 3 cut(s) 398, 428, 824
BfmI CTRYAG 2 cut(s) 414, 991
BglII AGATCT 2 cut(s) 12, 889
BisI GCNGC 3 cut(s) 66, 248, 566
BlsI GCNGC 3 cut(s) 67, 249, 567
Bme1390I CCNGG 6 cut(s) 200, 600, 672, 673, 743, 897
Bme18I GGWCC 3 cut(s) 264, 356, 998
BmeT110I CYCGRG 1 cut(s) 671
BmgT120I GGNCC 3 cut(s) 264, 356, 998
BmiI GGNNCC 2 cut(s) 284, 999
BmrFI CCNGG 6 cut(s) 200, 600, 672, 673, 743, 897
BmsI GCATC 1 cut(s) 303
BoxI GACNNNNGTC 1 cut(s) 99
Bpu14I TTCGAA 1 cut(s) 1009
BpuEI CTTGAG 3 cut(s) 494, 865, 915
BpuMI CCSGG 3 cut(s) 672, 673, 743
BsaJI CCNNGG 2 cut(s) 198, 671
BsaWI WCCGGW 2 cut(s) 89, 870
Bsc4I CCNNNNNNNGG 1 cut(s) 767
Bse118I RCCGGY 1 cut(s) 700
Bse1I ACTGG 2 cut(s) 461, 662
BseAI TCCGGA 1 cut(s) 870
BseBI CCWGG 3 cut(s) 200, 600, 897
BseDI CCNNGG 2 cut(s) 198, 671
BseGI GGATG 2 cut(s) 595, 777
BseLI CCNNNNNNNGG 1 cut(s) 767
BseNI ACTGG 2 cut(s) 461, 662
BseRI GAGGAG 1 cut(s) 918
BseXI GCAGC 2 cut(s) 77, 259
BsgI GTGCAG 1 cut(s) 844
Bsh1236I CGCG 1 cut(s) 107
BshNI GGYRCC 1 cut(s) 282
BsiHKAI GWGCWC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 671
BsiSI CCGG 5 cut(s) 90, 672, 701, 743, 871
BslFI GGGAC 2 cut(s) 47, 87
BslI CCNNNNNNNGG 1 cut(s) 767
BsmAI GTCTC 1 cut(s) 310
BsmFI GGGAC 2 cut(s) 47, 87
BsmI GAATGC 1 cut(s) 536
BsoBI CYCGRG 1 cut(s) 671
Bsp119I TTCGAA 1 cut(s) 1009
Bsp1286I GDGCHC 2 cut(s) 207, 990
Bsp13I TCCGGA 1 cut(s) 870
Bsp143I GATC 5 cut(s) 3, 12, 487, 682, 889
BspACI CCGC 5 cut(s) 72, 105, 161, 566, 732
BspEI TCCGGA 1 cut(s) 870
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 2 cut(s) 284, 999
BspMAI CTGCAG 1 cut(s) 418
BspPI GGATC 2 cut(s) 495, 677
BspT104I TTCGAA 1 cut(s) 1009
BspT107I GGYRCC 1 cut(s) 282
BsrBI CCGCTC 1 cut(s) 734
BsrFI RCCGGY 1 cut(s) 700
BsrI ACTGG 2 cut(s) 461, 662
BssAI RCCGGY 1 cut(s) 700
BssECI CCNNGG 2 cut(s) 198, 671
BssMI GATC 5 cut(s) 3, 12, 487, 682, 889
Bst2UI CCWGG 3 cut(s) 200, 600, 897
Bst4CI ACNGT 3 cut(s) 287, 610, 751
Bst6I CTCTTC 3 cut(s) 123, 591, 942
BstBI TTCGAA 1 cut(s) 1009
BstC8I GCNNGC 4 cut(s) 70, 414, 563, 620
BstF5I GGATG 2 cut(s) 595, 777
BstFNI CGCG 1 cut(s) 107
BstKTI GATC 5 cut(s) 6, 15, 490, 685, 892
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 5 cut(s) 3, 12, 487, 682, 889
BstMWI GCNNNNNNNGC 2 cut(s) 256, 701
BstNI CCWGG 3 cut(s) 200, 600, 897
BstNSI RCATGY 1 cut(s) 565
BstPAI GACNNNNGTC 1 cut(s) 99
BstSCI CCNGG 6 cut(s) 198, 598, 670, 671, 741, 895
BstSFI CTRYAG 2 cut(s) 414, 991
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 2 cut(s) 77, 259
BstX2I RGATCY 2 cut(s) 12, 889
BstYI RGATCY 2 cut(s) 12, 889
BtgZI GCGATG 1 cut(s) 133
BtsCI GGATG 2 cut(s) 595, 777
BtsI GCAGTG 2 cut(s) 497, 856
BtsIMutI CAGTG 2 cut(s) 497, 856
Cac8I GCNNGC 4 cut(s) 70, 414, 563, 620
CaiI CAGNNNCTG 1 cut(s) 499
Cfr10I RCCGGY 1 cut(s) 700
Cfr13I GGNCC 3 cut(s) 264, 356, 998
Cfr9I CCCGGG 1 cut(s) 671
Csp6I GTAC 3 cut(s) 283, 449, 633
CviAII CATG 5 cut(s) 186, 244, 298, 527, 562
CviQI GTAC 3 cut(s) 283, 449, 633
DpnI GATC 5 cut(s) 5, 14, 489, 684, 891
DpnII GATC 5 cut(s) 3, 12, 487, 682, 889
Eam1104I CTCTTC 3 cut(s) 123, 591, 942
EarI CTCTTC 3 cut(s) 123, 591, 942
Eco24I GRGCYC 1 cut(s) 990
Eco47I GGWCC 3 cut(s) 264, 356, 998
Eco57I CTGAAG 1 cut(s) 864
Eco88I CYCGRG 1 cut(s) 671
EcoO109I RGGNCCY 1 cut(s) 998
EcoRII CCWGG 3 cut(s) 198, 598, 895
EcoT38I GRGCYC 1 cut(s) 990
FaeI CATG 5 cut(s) 189, 247, 301, 530, 565
FaqI GGGAC 2 cut(s) 47, 87
FatI CATG 5 cut(s) 185, 243, 297, 526, 561
FauI CCCGC 2 cut(s) 112, 168
FauNDI CATATG 1 cut(s) 652
FblI GTMKAC 1 cut(s) 853
Fnu4HI GCNGC 3 cut(s) 66, 248, 566
FokI GGATG 2 cut(s) 582, 764
FriOI GRGCYC 1 cut(s) 990
Fsp4HI GCNGC 3 cut(s) 66, 248, 566
FspBI CTAG 3 cut(s) 398, 428, 824
GluI GCNGC 3 cut(s) 66, 248, 566
HapII CCGG 5 cut(s) 90, 672, 701, 743, 871
Hin1II CATG 5 cut(s) 189, 247, 301, 530, 565
HincII GTYRAC 1 cut(s) 854
HindII GTYRAC 1 cut(s) 854
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 6 cut(s) 86, 100, 318, 552, 918, 976
HpaII CCGG 5 cut(s) 90, 672, 701, 743, 871
HphI GGTGA 2 cut(s) 104, 911
Hpy166II GTNNAC 1 cut(s) 854
Hpy188I TCNGA 8 cut(s) 54, 85, 315, 367, 691, 716, 844, 981
Hpy188III TCNNGA 5 cut(s) 18, 428, 871, 882, 962
Hpy8I GTNNAC 1 cut(s) 854
HpyAV CCTTC 2 cut(s) 192, 579
HpyCH4III ACNGT 3 cut(s) 287, 610, 751
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 7 cut(s) 247, 294, 333, 416, 520, 561, 861
HpyF10VI GCNNNNNNNGC 2 cut(s) 256, 701
HpySE526I ACGT 1 cut(s) 36
Hsp92II CATG 5 cut(s) 189, 247, 301, 530, 565
Kpn2I TCCGGA 1 cut(s) 870
KpnI GGTACC 1 cut(s) 286
Kzo9I GATC 5 cut(s) 3, 12, 487, 682, 889
LmnI GCTCC 3 cut(s) 739, 832, 931
Lsp1109I GCAGC 2 cut(s) 77, 259
LweI GCATC 1 cut(s) 303
MaeI CTAG 3 cut(s) 398, 428, 824
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 4 cut(s) 92, 495, 919, 934
MalI GATC 5 cut(s) 5, 14, 489, 684, 891
MbiI CCGCTC 1 cut(s) 734
MboI GATC 5 cut(s) 3, 12, 487, 682, 889
MboII GAAGA 5 cut(s) 110, 380, 578, 857, 959
MflI RGATCY 2 cut(s) 12, 889
MhlI GDGCHC 2 cut(s) 207, 990
MluCI AATT 2 cut(s) 405, 646
MlyI GAGTC 3 cut(s) 109, 312, 927
MroI TCCGGA 1 cut(s) 870
MroXI GAANNNNTTC 2 cut(s) 384, 951
MseI TTAA 3 cut(s) 275, 408, 645
MslI CAYNNNNRTG 1 cut(s) 525
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 5 cut(s) 90, 672, 701, 743, 871
MspR9I CCNGG 6 cut(s) 200, 600, 672, 673, 743, 897
Mva1269I GAATGC 1 cut(s) 536
MvaI CCWGG 3 cut(s) 200, 600, 897
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 2 cut(s) 256, 701
NciI CCSGG 3 cut(s) 672, 673, 743
NdeI CATATG 1 cut(s) 652
NdeII GATC 5 cut(s) 3, 12, 487, 682, 889
NlaIII CATG 5 cut(s) 189, 247, 301, 530, 565
NlaIV GGNNCC 2 cut(s) 284, 999
NmuCI GTSAC 4 cut(s) 92, 495, 919, 934
NspI RCATGY 1 cut(s) 565
NspV TTCGAA 1 cut(s) 1009
PaeI GCATGC 1 cut(s) 565
PctI GAATGC 1 cut(s) 536
PdmI GAANNNNTTC 2 cut(s) 384, 951
PfeI GAWTC 3 cut(s) 86, 552, 976
PfoI TCCNGGA 1 cut(s) 598
PkrI GCNGC 3 cut(s) 67, 249, 567
PleI GAGTC 3 cut(s) 108, 312, 926
PpsI GAGTC 3 cut(s) 108, 312, 926
PpuMI RGGWCCY 1 cut(s) 998
PshAI GACNNNNGTC 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 998
Psp6I CCWGG 3 cut(s) 198, 598, 895
PspGI CCWGG 3 cut(s) 198, 598, 895
PspN4I GGNNCC 2 cut(s) 284, 999
PspPI GGNCC 3 cut(s) 264, 356, 998
PspPPI RGGWCCY 1 cut(s) 998
PstI CTGCAG 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 499
PsuI RGATCY 2 cut(s) 12, 889
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 3 cut(s) 284, 450, 634
RsaNI GTAC 3 cut(s) 283, 449, 633
RseI CAYNNNNRTG 1 cut(s) 525
SalI GTCGAC 1 cut(s) 852
SaqAI TTAA 3 cut(s) 275, 408, 645
SatI GCNGC 3 cut(s) 66, 248, 566
Sau3AI GATC 5 cut(s) 3, 12, 487, 682, 889
Sau96I GGNCC 3 cut(s) 264, 356, 998
SchI GAGTC 3 cut(s) 109, 312, 927
ScrFI CCNGG 6 cut(s) 200, 600, 672, 673, 743, 897
SduI GDGCHC 2 cut(s) 207, 990
SfaNI GCATC 1 cut(s) 303
SfcI CTRYAG 2 cut(s) 414, 991
SfuI TTCGAA 1 cut(s) 1009
SinI GGWCC 3 cut(s) 264, 356, 998
SmaI CCCGGG 1 cut(s) 673
SmiMI CAYNNNNRTG 1 cut(s) 525
SmlI CTYRAG 3 cut(s) 473, 880, 930
SmoI CTYRAG 3 cut(s) 473, 880, 930
SphI GCATGC 1 cut(s) 565
Sse9I AATT 2 cut(s) 405, 646
SsiI CCGC 5 cut(s) 72, 105, 161, 566, 732
SspMI CTAG 3 cut(s) 398, 428, 824
StyD4I CCNGG 6 cut(s) 198, 598, 670, 671, 741, 895
TaaI ACNGT 3 cut(s) 287, 610, 751
TaiI ACGT 1 cut(s) 39
TaqI TCGA 3 cut(s) 378, 853, 1009
TasI AATT 2 cut(s) 405, 646
TauI GCSGC 1 cut(s) 568
TfiI GAWTC 3 cut(s) 86, 552, 976
Tru1I TTAA 3 cut(s) 275, 408, 645
Tru9I TTAA 3 cut(s) 275, 408, 645
TscAI CASTG 2 cut(s) 504, 863
TseFI GTSAC 4 cut(s) 92, 495, 919, 934
TseI GCWGC 2 cut(s) 65, 247
Tsp45I GTSAC 4 cut(s) 92, 495, 919, 934
TspDTI ATGAA 6 cut(s) 136, 174, 242, 543, 578, 639
TspMI CCCGGG 1 cut(s) 671
TspRI CASTG 2 cut(s) 504, 863
VpaK11BI GGWCC 3 cut(s) 264, 356, 998
XbaI TCTAGA 1 cut(s) 427
XceI RCATGY 1 cut(s) 565
XmaI CCCGGG 1 cut(s) 671
XmiI GTMKAC 1 cut(s) 853
XmnI GAANNNNTTC 2 cut(s) 384, 951
XspI CTAG 3 cut(s) 398, 428, 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.