Rmu_sc0005690.1_g000001

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005690.1
OG0017411
Physical Location & Seq
Forward (+)
380 .. 1867
1488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005690.1_g000001.1.cds

Sequence Viewer

Length: 558 bp
atgactcgccatagccataatcatagccatgaaatcgagaatacaagcaaagatgaggacaagattatagacaagaagccaagaatacttgctgcatcagaatctaaaaccaccataactacacgctctcgcccccatgacaaccagctcacctacaccggcggcaagaccaagatcctcattgtcggacacaccatcgaattcctcgccctctccgacagtgacgtgtcgttcaagtaccagctccccggggaagacctcgacgccttgatctccgtcaccaaccacgacgacctcgagcacatgatgcacgagtacgatcgcctctacagggcctccgccaggatgaggttgtttctcttcccggtcaatcataacgacagtttcgagtctgacaagtcgtccgatagggattgcttcattgaggctttgaattcgggtcctgagataaaaataagaaggtggagtggagcctctccggctgagatgctaaatctggtggccagtatggaggaccacgacgtcgagcccaacacaagctgccttttttttttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

185

Amino Acids

21.18

Weight (kDa)

5.5

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 521
AatII GACGTC 1 cut(s) 525
AciI CCGC 2 cut(s) 162, 339
AclWI GGATC 1 cut(s) 169
AcoI YGGCCR 1 cut(s) 501
AcsI RAATTY 2 cut(s) 200, 433
AcyI GRCGYC 2 cut(s) 264, 522
AfaI GTAC 2 cut(s) 239, 317
AfiI CCNNNNNNNGG 3 cut(s) 331, 342, 348
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 2 cut(s) 235, 433
AhdI GACNNNNNGTC 1 cut(s) 400
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 1 cut(s) 341
AluBI AGCT 3 cut(s) 148, 244, 540
AluI AGCT 3 cut(s) 148, 244, 540
Alw21I GWGCWC 1 cut(s) 303
AlwI GGATC 1 cut(s) 169
Ama87I CYCGRG 2 cut(s) 248, 296
AoxI GGCC 2 cut(s) 333, 501
ApeKI GCWGC 2 cut(s) 92, 540
ApoI RAATTY 2 cut(s) 200, 433
AspS9I GGNCC 3 cut(s) 333, 440, 514
AsuC2I CCSGG 3 cut(s) 249, 250, 365
AsuHPI GGTGA 2 cut(s) 142, 271
AvaI CYCGRG 2 cut(s) 248, 296
AvaII GGWCC 2 cut(s) 440, 514
BalI TGGCCA 1 cut(s) 503
BanII GRGCYC 1 cut(s) 531
BauI CACGAG 1 cut(s) 311
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 2 cut(s) 79, 527
BccI CCATC 1 cut(s) 203
BciT130I CCWGG 1 cut(s) 343
BcnI CCSGG 3 cut(s) 249, 250, 365
BfmI CTRYAG 1 cut(s) 328
BglI GCCNNNNNGGC 1 cut(s) 479
BisI GCNGC 3 cut(s) 93, 163, 541
BlsI GCNGC 3 cut(s) 94, 164, 542
Bme1390I CCNGG 4 cut(s) 249, 250, 343, 365
Bme18I GGWCC 2 cut(s) 440, 514
BmeRI GACNNNNNGTC 1 cut(s) 400
BmeT110I CYCGRG 2 cut(s) 248, 296
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 3 cut(s) 333, 440, 514
BmiI GGNNCC 2 cut(s) 441, 472
BmrFI CCNGG 4 cut(s) 249, 250, 343, 365
BmsI GCATC 3 cut(s) 104, 297, 477
BpiI GAAGAC 1 cut(s) 261
BpuMI CCSGG 3 cut(s) 249, 250, 365
BsaHI GRCGYC 2 cut(s) 264, 522
BsaJI CCNNGG 3 cut(s) 247, 248, 249
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 3 cut(s) 331, 342, 348
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 1 cut(s) 504
BseBI CCWGG 1 cut(s) 343
BseDI CCNNGG 3 cut(s) 247, 248, 249
BseGI GGATG 1 cut(s) 351
BseLI CCNNNNNNNGG 3 cut(s) 331, 342, 348
BseMII CTCAG 2 cut(s) 435, 474
BseNI ACTGG 1 cut(s) 504
BseXI GCAGC 2 cut(s) 79, 527
Bsh1285I CGRYCG 1 cut(s) 322
BshFI GGCC 2 cut(s) 335, 503
BsiEI CGRYCG 1 cut(s) 322
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 2 cut(s) 248, 296
BsiSI CCGG 4 cut(s) 159, 249, 365, 479
BslI CCNNNNNNNGG 3 cut(s) 331, 342, 348
BsnI GGCC 2 cut(s) 335, 503
BsoBI CYCGRG 2 cut(s) 248, 296
Bsp1286I GDGCHC 2 cut(s) 303, 531
Bsp143I GATC 3 cut(s) 174, 270, 319
BspACI CCGC 2 cut(s) 162, 339
BspANI GGCC 2 cut(s) 335, 503
BspCNI CTCAG 2 cut(s) 436, 475
BspLI GGNNCC 2 cut(s) 441, 472
BspPI GGATC 1 cut(s) 169
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 1 cut(s) 504
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 3 cut(s) 247, 248, 249
BssMI GATC 3 cut(s) 174, 270, 319
BssNI GRCGYC 2 cut(s) 264, 522
BssSI CACGAG 1 cut(s) 311
Bst2BI CACGAG 1 cut(s) 311
Bst2UI CCWGG 1 cut(s) 343
Bst4CI ACNGT 2 cut(s) 221, 383
Bst6I CTCTTC 1 cut(s) 365
BstACI GRCGYC 2 cut(s) 264, 522
BstAPI GCANNNNNTGC 1 cut(s) 307
BstDEI CTNAG 2 cut(s) 444, 483
BstENI CCTNNNNNAGG 1 cut(s) 340
BstF5I GGATG 1 cut(s) 351
BstKTI GATC 3 cut(s) 177, 273, 322
BstMBI GATC 3 cut(s) 174, 270, 319
BstMCI CGRYCG 1 cut(s) 322
BstMWI GCNNNNNNNGC 2 cut(s) 307, 479
BstNI CCWGG 1 cut(s) 343
BstSCI CCNGG 4 cut(s) 247, 248, 341, 363
BstSFI CTRYAG 1 cut(s) 328
BstV1I GCAGC 2 cut(s) 79, 527
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 174
BstYI RGATCY 1 cut(s) 174
BsuRI GGCC 2 cut(s) 335, 503
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 1 cut(s) 351
BtsIMutI CAGTG 1 cut(s) 226
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 3 cut(s) 333, 440, 514
Cfr9I CCCGGG 1 cut(s) 248
CseI GACGC 1 cut(s) 272
Csp6I GTAC 2 cut(s) 238, 316
CviAII CATG 3 cut(s) 29, 137, 304
CviQI GTAC 2 cut(s) 238, 316
DdeI CTNAG 2 cut(s) 444, 483
DpnI GATC 3 cut(s) 176, 272, 321
DpnII GATC 3 cut(s) 174, 270, 319
DrdI GACNNNNNNGTC 1 cut(s) 521
DriI GACNNNNNGTC 1 cut(s) 400
DseDI GACNNNNNNGTC 1 cut(s) 521
EaeI YGGCCR 1 cut(s) 501
Eam1104I CTCTTC 1 cut(s) 365
Eam1105I GACNNNNNGTC 1 cut(s) 400
EarI CTCTTC 1 cut(s) 365
EciI GGCGGA 1 cut(s) 328
Eco24I GRGCYC 1 cut(s) 531
Eco47I GGWCC 2 cut(s) 440, 514
Eco88I CYCGRG 2 cut(s) 248, 296
EcoNI CCTNNNNNAGG 1 cut(s) 340
EcoO109I RGGNCCY 2 cut(s) 333, 440
EcoRI GAATTC 2 cut(s) 200, 433
EcoRII CCWGG 1 cut(s) 341
EcoT38I GRGCYC 1 cut(s) 531
FaeI CATG 3 cut(s) 32, 140, 307
FatI CATG 3 cut(s) 28, 136, 303
Fnu4HI GCNGC 3 cut(s) 93, 163, 541
FokI GGATG 1 cut(s) 358
FriOI GRGCYC 1 cut(s) 531
Fsp4HI GCNGC 3 cut(s) 93, 163, 541
GluI GCNGC 3 cut(s) 93, 163, 541
HaeIII GGCC 2 cut(s) 335, 503
HapII CCGG 4 cut(s) 159, 249, 365, 479
HgaI GACGC 1 cut(s) 272
Hin1I GRCGYC 2 cut(s) 264, 522
Hin1II CATG 3 cut(s) 32, 140, 307
HinfI GANTC 3 cut(s) 4, 101, 389
HpaII CCGG 4 cut(s) 159, 249, 365, 479
HphI GGTGA 2 cut(s) 142, 271
Hpy188I TCNGA 5 cut(s) 100, 188, 217, 394, 406
Hpy188III TCNNGA 2 cut(s) 37, 443
Hpy99I CGWCG 4 cut(s) 266, 293, 524, 527
HpyAV CCTTC 1 cut(s) 453
HpyCH4III ACNGT 2 cut(s) 221, 383
HpyCH4IV ACGT 2 cut(s) 225, 522
HpyCH4V TGCA 2 cut(s) 95, 310
HpyF10VI GCNNNNNNNGC 2 cut(s) 307, 479
HpyF3I CTNAG 2 cut(s) 444, 483
HpySE526I ACGT 2 cut(s) 225, 522
Hsp92I GRCGYC 2 cut(s) 264, 522
Hsp92II CATG 3 cut(s) 32, 140, 307
Kzo9I GATC 3 cut(s) 174, 270, 319
LmnI GCTCC 2 cut(s) 249, 470
Lsp1109I GCAGC 2 cut(s) 79, 527
LweI GCATC 3 cut(s) 104, 297, 477
MaeII ACGT 2 cut(s) 225, 522
MaeIII GTNAC 2 cut(s) 221, 277
MalI GATC 3 cut(s) 176, 272, 321
MboI GATC 3 cut(s) 174, 270, 319
MboII GAAGA 2 cut(s) 266, 352
MflI RGATCY 1 cut(s) 174
MhlI GDGCHC 2 cut(s) 303, 531
MlsI TGGCCA 1 cut(s) 503
MluCI AATT 2 cut(s) 200, 433
MluNI TGGCCA 1 cut(s) 503
MlyI GAGTC 1 cut(s) 398
MmeI TCCRAC 2 cut(s) 166, 240
Mox20I TGGCCA 1 cut(s) 503
MscI TGGCCA 1 cut(s) 503
MslI CAYNNNNRTG 1 cut(s) 27
Msp20I TGGCCA 1 cut(s) 503
MspI CCGG 4 cut(s) 159, 249, 365, 479
MspR9I CCNGG 4 cut(s) 249, 250, 343, 365
MvaI CCWGG 1 cut(s) 343
MwoI GCNNNNNNNGC 2 cut(s) 307, 479
NciI CCSGG 3 cut(s) 249, 250, 365
NdeII GATC 3 cut(s) 174, 270, 319
NlaIII CATG 3 cut(s) 32, 140, 307
NlaIV GGNNCC 2 cut(s) 441, 472
NmuCI GTSAC 2 cut(s) 221, 277
PaeR7I CTCGAG 1 cut(s) 296
PcsI WCGNNNNNNNCGW 3 cut(s) 213, 294, 384
PfeI GAWTC 1 cut(s) 101
PkrI GCNGC 3 cut(s) 94, 164, 542
Ple19I CGATCG 1 cut(s) 322
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
PpuMI RGGWCCY 1 cut(s) 440
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 341
PspGI CCWGG 1 cut(s) 341
PspN4I GGNNCC 2 cut(s) 441, 472
PspPI GGNCC 3 cut(s) 333, 440, 514
PspPPI RGGWCCY 1 cut(s) 440
PspXI VCTCGAGB 1 cut(s) 296
PsuI RGATCY 1 cut(s) 174
PvuI CGATCG 1 cut(s) 322
RsaI GTAC 2 cut(s) 239, 317
RsaNI GTAC 2 cut(s) 238, 316
RseI CAYNNNNRTG 1 cut(s) 27
SatI GCNGC 3 cut(s) 93, 163, 541
Sau3AI GATC 3 cut(s) 174, 270, 319
Sau96I GGNCC 3 cut(s) 333, 440, 514
SchI GAGTC 1 cut(s) 398
ScrFI CCNGG 4 cut(s) 249, 250, 343, 365
SduI GDGCHC 2 cut(s) 303, 531
SfaNI GCATC 3 cut(s) 104, 297, 477
SfcI CTRYAG 1 cut(s) 328
Sfr274I CTCGAG 1 cut(s) 296
SgrAI CRCCGGYG 1 cut(s) 158
SinI GGWCC 2 cut(s) 440, 514
SlaI CTCGAG 1 cut(s) 296
SmaI CCCGGG 1 cut(s) 250
SmiMI CAYNNNNRTG 1 cut(s) 27
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 2 cut(s) 200, 433
SsiI CCGC 2 cut(s) 162, 339
StyD4I CCNGG 4 cut(s) 247, 248, 341, 363
TaaI ACNGT 2 cut(s) 221, 383
TaiI ACGT 2 cut(s) 228, 525
TaqI TCGA 6 cut(s) 36, 198, 261, 297, 387, 525
TasI AATT 2 cut(s) 200, 433
TauI GCSGC 1 cut(s) 165
TfiI GAWTC 1 cut(s) 101
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 2 cut(s) 221, 277
TseI GCWGC 2 cut(s) 92, 540
Tsp45I GTSAC 2 cut(s) 221, 277
TspDTI ATGAA 2 cut(s) 45, 409
TspGWI ACGGA 1 cut(s) 265
TspMI CCCGGG 1 cut(s) 248
TspRI CASTG 1 cut(s) 226
VpaK11BI GGWCC 2 cut(s) 440, 514
XagI CCTNNNNNAGG 1 cut(s) 340
XapI RAATTY 2 cut(s) 200, 433
XhoI CTCGAG 1 cut(s) 296
XmaI CCCGGG 1 cut(s) 248
ZraI GACGTC 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.